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https://www.readbyqxmd.com/read/29793207/infectivity-reduction-efficacy-of-uv-irradiation-and-peracetic-acid-uv-combined-treatment-on-ms2-bacteriophage-and-murine-norovirus-in-secondary-wastewater-effluent
#1
ShihChi Weng, Nathan Dunkin, Kellogg J Schwab, James McQuarrie, Kati Bell, Joseph G Jacangelo
Peracetic acid (PAA) is a strong oxidant/bactericide that has been applied in various industries (e.g., food processing, pharmaceuticals, medical device sterilization, etc.) as a disinfectant. There is increasing interest in using PAA for wastewater disinfection because it does not form halogenated byproducts, and no post-treatment quenching is required. Previous studies have demonstrated good efficiency in controlling bacteria in wastewater, but limited information is available for viruses, especially those hosted by mammals (e...
May 21, 2018: Journal of Environmental Management
https://www.readbyqxmd.com/read/29792942/optimisation-of-decontamination-method-and-influence-of-culture-media-on-the-recovery-of-mycobacterium-avium-subspecies-paratuberculosis-from-spiked-water-sediments
#2
G Aboagye, M T Rowe
The recovery of Mycobacterium avium subspecies paratuberculosis (Map) from the environment can be a laborious process - owing to Map being fastidious, its low number, and also high numbers of other microbial populations in such settings. Protocols i.e. filtration, decontamination and modified elution were devised to recover Map from spiked water sediments. Three culture media: Herrold's Egg Yolk Media (HEYM), Middlebrook 7H10 (M-7H10) and Bactec 12B were then employed to grow the organism following its elution...
May 21, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29791712/surgical-management-after-hysteroscopic-sterilization-minimally-invasive-approach-incorporating-intraoperative-fluoroscopy-for-symptomatic-patients-with-2-essure%C3%A2-devices
#3
E Scott Sills, Natalie S Rickers, Xiang Li
OBJECTIVE: To describe a non-hysterectomy surgical technique for symptomatic patients with >2 Essure® (Bayer Healthcare, Whippany, New Jersey) devices. DESIGN: Patients (n=4) presented with sharp pelvic pain, irregular vaginal bleeding, dyspareunia, weight gain, hair loss, fatigue, and/or diffuse skin rash, all of which were absent before undergoing hysteroscopic sterilization (HS). Hysterosalpingogram obtained before surgical excision of contraceptive tubal implants confirmed more than two Essure® devices in all patients...
June 1, 2018: Surgical Technology International
https://www.readbyqxmd.com/read/29791436/the-genomic-landscape-at-a-late-stage-of-stickleback-speciation-high-genomic-divergence-interspersed-by-small-localized-regions-of-introgression
#4
Mark Ravinet, Kohta Yoshida, Shuji Shigenobu, Atsushi Toyoda, Asao Fujiyama, Jun Kitano
Speciation is a continuous process and analysis of species pairs at different stages of divergence provides insight into how it unfolds. Previous genomic studies on young species pairs have revealed peaks of divergence and heterogeneous genomic differentiation. Yet less known is how localised peaks of differentiation progress to genome-wide divergence during the later stages of speciation in the presence of persistent gene flow. Spanning the speciation continuum, stickleback species pairs are ideal for investigating how genomic divergence builds up during speciation...
May 23, 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29791311/protective-effects-of-persian-honey-apis-mellifera-meda-skorikov-on-side-effects-of-chemotherapy-and-ischemia-reperfusion-induced-testicular-injury
#5
Mohammadreza Gholami, Abolfazl Abbaszadeh, Parastoo Baharvand, Afshin Hasanvand, Amin Hasanvand, Anneh Mohammad Gharravi
Introduction The aim of the present study was to survey the protective effect of pretreatment with Persian honey on amelioration of side effects of chemotherapy and ischemia/reperfusion induced testicular injury. Materials and methods Forty adult's male wistar rats were divided into four groups of ischemia-reperfusion (IR), honey + ischemia-reperfusion (HIR), Busulfan (B) and Busulfan intraperitoneally+ honey (BH). The seminiferous tubules were rated for their modified spermatogenesis index (SI) by Johnsons score...
May 23, 2018: Journal of Complementary & Integrative Medicine
https://www.readbyqxmd.com/read/29791204/role-of-adenosine-signaling-in-coordinating-cardiomyocyte-function-and-coronary-vascular-growth-in-chronic-fetal-anemia
#6
Lowell Davis, James Musso, Divya Soman, Samantha Louey, Jonathan William Nelson, Sonnet S Jonker
Fetal anemia causes rapid and profound changes in cardiac structure and function, stimulating proliferation of the cardiac myocytes, expansion of the coronary vascular tree, and impairing early contraction and relaxation. While HIF-1α is sure to play a role, adenosine, a metabolic byproduct that increases coronary flow and growth, is implicated as a major stimulus for these adaptations. We hypothesized that genes involved in myocardial adenosine signaling would be up-regulated in chronically anemic fetuses, and that calcium handling genes would be down-regulated...
May 23, 2018: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/29790580/preclinical-evaluation-of-potential-infection-imaging-probe-68-ga-ga-dota-k-a9-in-sterile-and-infectious-inflammation
#7
Karin M Nielsen, Nis P Jørgensen, Majbritt H Kyneb, Per Borghammer, Rikke L Meyer, Trine R Thomsen, Dirk Bender, Svend B Jensen, Ole L Nielsen, Aage K O Alstrup
The development of bacteria-specific infection radiotracers is of considerable interest to improve diagnostic accuracy and enabling therapy monitoring. The aim of this study was to determine if the previously reported radiolabelled 1,4,7,10-tetraazacyclododecane-N,N',N″,N‴-tetraacetic acid (DOTA) conjugated peptide, [68 Ga]Ga-DOTA-K-A9 could detect a staphylococcal infection in vivo, and distinguish it from aseptic inflammation. An optimised [68 Ga]Ga-DOTA-K-A9 synthesis omitting the use of acetone was developed, yielding 93% ± 0...
May 23, 2018: Journal of Labelled Compounds & Radiopharmaceuticals
https://www.readbyqxmd.com/read/29790392/interleukin-1-receptor-il-1r1-activation-exacerbates-toxin-induced-acute-kidney-injury
#8
Jamie R Privratsky, Jiandong Zhang, Xiaohan Lu, Nathan Rudemiller, Qingqing Wei, Yen-Rei Yu, Michael Dee Gunn, Steven D Crowley
Acute kidney injury (AKI) is a leading cause of morbidity and mortality. Cisplatin is an effective chemotherapeutic agent whose administration is limited by nephrotoxicity. Therapies to prevent cisplatin-induced AKI are lacking. While tumor necrosis factor-α (TNF) plays a key role in the pathogenesis of cisplatin nephrotoxicity, the immune signaling pathways that trigger TNF generation in this context require elucidation. Sterile injury triggers the release and activation of both isoforms of interleukin(IL)-1, IL-1α and IL-1β, and stimulation of the interleukin-1 receptor (IL-1R1) by these ligands engages a pro-inflammatory signaling cascade that induces TNF induction...
May 23, 2018: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/29790257/functionalized-silk-fibers-from-transgenic-silkworms-for-wound-healing-applications-surface-presentation-of-bioactive-epidermal-growth-factor
#9
Michael Wöltje, Melanie Böbel, Michaela Bienert, Sabine Neuss, Dilibaier Aibibu, Chokri Cherif
Growth factors play a crucial role in wound healing in general and are promising tools for the treatment of chronic wounds as they can restore the physiological wound healing process. In growth factor-loaded wound dressings, human epidermal growth factor (EGF) is released in a burst and washed out quickly. The developed matrix consists of recombinant EGF produced in transgenic silkworms as a fusion protein with the fibroin light chain. The covalent linkage prevents EGF from draining into the surrounding tissue while presenting the growth factor on the surface...
May 23, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29789975/exploring-potential-applications-of-a-novel-extracellular-polymeric-substance-synthesizing-bacterium-bacillus-licheniformis-isolated-from-gut-contents-of-earthworm-metaphire-posthuma-in-environmental-remediation
#10
Jayanta Kumar Biswas, Anurupa Banerjee, Mahendra Kumar Rai, Jörg Rinklebe, Sabry M Shaheen, Santosh Kumar Sarkar, Madhab Chandra Dash, Anilava Kaviraj, Uwe Langer, Hocheol Song, Meththika Vithanage, Monojit Mondal, Nabeel Khan Niazi
The aim was to isolate, characterize, and explore potentials of gut bacteria from the earthworm (Metaphire posthuma) and imply these bacteria for remediation of Cu(II) and Zn(II). An extracellular polymeric substance (EPS) producing gut bacteria (Bacillus licheniformis strain KX657843) was isolated and identified based on 16S rRNA sequencing and phylogenetic analysis. The strain showed maximum tolerance of 8 and 6 mM for Cu(II) and Zn(II) respectively. It removed 34.5% of Cu(II) and 54.4% of Zn(II) at 25 mg L-1 after 72 and 96 h incubation respectively...
May 22, 2018: Biodegradation
https://www.readbyqxmd.com/read/29788142/molecular-characterization-of-the-2016-new-world-screwworm-diptera-calliphoridae-outbreak-in-the-florida-keys
#11
Julian R Dupuis, Felix D Guerrero, Steven R Skoda, Pamela L Phillips, John B Welch, Jack L Schlater, Ana Maria L Azeredo-Espin, Adalberto A Pérez de León, Scott M Geib
New World screwworm (NWS), Cochliomyia hominivorax (Coquerel 1858) (Diptera: Calliphoridae), is a myiasis-causing fly that can be a serious threat to the health of livestock, wildlife, and humans. Its progressive eradication from the southern United States, Mexico, and Central America from the 1950s to 2000s is an excellent example of successful pest management using sterile insect technique (SIT). In late 2016, autochthonous NWS were detected in the Florida Keys, representing this species' first invasion in the United States in >30 yr...
May 19, 2018: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29788108/porphyromonas-gingivalis-strain-dependent-inhibition-of-uterine-spiral-artery-remodeling-in-the-pregnant-rat
#12
P Phillips, M B Brown, A Progulske-Fox, X J Wu, L Reyes
Porphyromonas gingivalis (Pg), is an important periodontal pathogen that is also implicated in pregnancy complications involving defective deep placentation (DDP). We hypothesized that Pg invasion of the placental bed promotes DDP. Pregnant rats were intravenously inoculated with sterile vehicle, Pg strain W83, or A7436 at gestation day (GD) 14 (acute cohort). Non-pregnant rats received repeated oral inoculations for 3 months before breeding (chronic cohort). Tissues and/or sera were collected at GD18 for analysis...
May 18, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29787520/early-tlr4-blockade-attenuates-sterile-inflammation-mediated-stress-in-islets-during-isolation-and-promotes-successful-transplant-outcomes
#13
Charles A Chang, Kayla Murphy, Robert R Kane, Michael Lawrence, Bashoo Naziruddin
BACKGROUND: During the isolation process, pancreatic islets are exposed to an environment of sterile inflammation resulting in an upregulated inflammatory state prior to transplantation. Toll-like receptor 4 (TLR4) has been identified as a major mediator of sterile inflammation. Therefore, we sought to determine whether early TLR4 blockade would be effective in reducing the inflammatory burden in islets pretransplant. METHODS: Islets from C57BL/6 mice were treated with a TLR4 antagonist during the pancreatic ductal perfusion and digestion steps of the isolation process...
May 22, 2018: Transplantation
https://www.readbyqxmd.com/read/29787299/the-influences-of-thermal-processing-on-phytochemicals-and-possible-routes-to-the-discovery-of-new-phytochemical-conjugates
#14
Fai-Chu Wong, Tsun-Thai Chai, Jianbo Xiao
In our diets, many of the consumed foods are subjected to various forms of heating and thermal processing. Besides enhancing the taste, texture, and aroma of the foods, heating helps to sterilize and facilitate food storage. On the other hand, heating and thermal processing are frequently reported during the preparation of various traditional herbal medicines. In this review, we intend to highlight works by various research groups which reported on changes in phytochemicals and bioactivities, following thermal processing of selected plant-derived foods and herbal medicines...
May 22, 2018: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/29786908/opposing-patterns-of-intraspecific-and-interspecific-differentiation-in-sex-chromosomes-and-autosomes
#15
Peter A Moran, Sonia Pascoal, Timothee Cezard, Judith E Risse, Michael G Ritchie, Nathan W Bailey
Linking intraspecific and interspecific divergence is an important challenge in speciation research. X chromosomes are expected to evolve faster than autosomes and disproportionately contribute to reproductive barriers, and comparing genetic variation on X and autosomal markers within and between species can elucidate evolutionary processes that shape genome variation. We performed RADseq on a 16-population transect of two closely-related Australian cricket species, Teleogryllus commodus and T. oceanicus, covering allopatry and sympatry...
May 22, 2018: Molecular Ecology
https://www.readbyqxmd.com/read/29785948/-genetics-of-fertility-restoration-in-the-isocytoplasm-allonuclear-c-group-of-cytoplasmic-male-sterility-in-maize
#16
Zhuo Fan Zhao, Ling Huang, Yong Ming Liu, Peng Zhang, Gui Wei, Mo Ju Cao
Maize is one of the first crops to produce hybrids using cytoplasmic male sterile lines. The C-type cytoplasmic male sterile (CMS-C) line is vital for hybrid seed production, and the fertility-restoration reaction along with its stability has a direct bearing on its applications. However, fertility restoration mechanism of CMS-C is complex and is still not clear so far. To further explore the factors affecting the fertility restoration of maize CMS, a series of test crosses were carried out by pollinating the isocytoplasm allonuclear CMS-C lines C48-2, Chuangzaosi and C478 with the test lines 18 bai, zi 330, 5022 and the restorer line A619, respectively...
May 20, 2018: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/29785324/-staphylococcus-aureus-is-the-most-common-bacterial-agent-of-the-skin-flora-of-patients-with-seborrheic-dermatitis
#17
Funda Tamer, Mehmet Eren Yuksel, Evren Sarifakioglu, Yavuz Karabag
Background: Seborrheic dermatitis is an inflammatory skin disease that affects 1-3% of the general population. The Malassezia species has been implicated as the main causative agent; however, the bacterial flora of the skin may also play role in the etiopathogenesis. Therefore, we investigated the most common bacterial agent of the skin flora of patients with seborrheic dermatitis. Materials and Methods: Fifty-one patients with seborrheic dermatitis and 50 healthy individuals are included in this study...
April 2018: Dermatology Practical & Conceptual
https://www.readbyqxmd.com/read/29785273/gene-regulation-associated-with-sexual-development-and-female-fertility-in-different-isolates-of-trichoderma-reesei
#18
Christoph Dattenböck, Doris Tisch, Andre Schuster, Alberto Alonso Monroy, Wolfgang Hinterdobler, Monika Schmoll
Background: Trichoderma reesei is one of the most frequently used filamentous fungi in industry for production of homologous and heterologous proteins. The ability to use sexual crossing in this fungus was discovered several years ago and opens up new perspectives for industrial strain improvement and investigation of gene regulation. Results: Here we investigated the female sterile strain QM6a in comparison to the fertile isolate CBS999.97 and backcrossed derivatives of QM6a, which have regained fertility (FF1 and FF2 strains) in both mating types under conditions of sexual development...
2018: Fungal Biology and Biotechnology
https://www.readbyqxmd.com/read/29785162/evaluation-of-chromatic-changes-of-a-nanocomposite-resin-using-the-new-whitness-index
#19
Diana Carla Mada, Cristina Gasparik, Alexandra Iulia Irimie, Marius Dan Mada, Diana Dudea, Radu Septimiu Campian
Background and aims: To evaluate the staining effects of two brands of coffee and the bleaching efficiency of two in-office bleaching methods, upon different opacities of a commercial nanocomposite. Methods: Twenty four specimens of each opacity, A3 Dentin, A3 Body and A3 Enamel, were fabricated from Filtek Supreme (3MEspe). The specimens were further divided into two groups (n=12) and were immersed in two coffee solutions (Bio Organic Coffee Bellarom, 100% Arabica, and Iulius Meinl Coffee), for 24 hours...
2018: Clujul Medical (1957)
https://www.readbyqxmd.com/read/29785086/the-utilization-of-an-ocular-wound-chamber-on-corneal-epithelial-wounds
#20
Jennifer S McDaniel, Andrew W Holt, Elaine D Por, Elof Eriksson, Anthony J Johnson, Gina L Griffith
Purpose: Currently available ocular moisture chambers are not adequate to manage the treatment of periocular burns, corneal injuries, and infection. The purpose of these studies was to demonstrate that a flexible, semi-transparent ocular wound chamber device adapted from technology currently used on dermal wounds is safe for use on corneal epithelial injuries. Materials and methods: A depilatory cream (Nair™, 30 seconds) was utilized to remove the excess hair surrounding the left eyes of anesthetized Institute Armand Frappier (IAF) hairless, female guinea pigs (Crl:HA-Hrhr)...
2018: Clinical Ophthalmology
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