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https://www.readbyqxmd.com/read/28231436/myocardial-homing-of-mesenchymal-stem-cells-following-intrapericardial-application-and-amplification-by-inflammation-an-experimental-pilot-study
#1
Nina Jung, Heinz Rupp, Rembert Koczulla, Claus Vogelmeier, Peter Alter
Recent studies demonstrated potential effects of stem cells on cardiac function in heart failure. However, influences of the technique of application remained undetermined. In the present study, the pericardial sac was used as depot for fluorescent-labeled mesenchymal stem cells in rats. To evaluate influences of inflammation on cell homing, a sterile pericarditis was induced by talc. It is shown that intrapericardial stem cells application is sufficient to provide myocardial penetration. The extent of homing was amplified by inflammation in a talc-induced pericarditis...
February 23, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28231407/co-occurrence-and-hybridization-of-anther-smut-pathogens-specialized-on-dianthus-hosts
#2
Elsa Petit, Casey Silver, Amandine Cornille, Pierre Gladieux, Lisa Rosenthal, Emily Bruns, Sarah Yee, Janis Antonovics, Tatiana Giraud, Michael E Hood
Host specialization has important consequences for the diversification and ecological interactions of obligate pathogens. The anther-smut disease of natural plant populations, caused by Microbotryum fungi, has been characterized by specialized host-pathogen interactions, which contribute in part to the isolation among these numerous fungal species. This study investigated the molecular variation of Microbotryum pathogens within the geographic and host-specific distributions on wild Dianthus species in southern European Alps...
February 23, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28231323/even-good-bots-fight-the-case-of-wikipedia
#3
Milena Tsvetkova, Ruth García-Gavilanes, Luciano Floridi, Taha Yasseri
In recent years, there has been a huge increase in the number of bots online, varying from Web crawlers for search engines, to chatbots for online customer service, spambots on social media, and content-editing bots in online collaboration communities. The online world has turned into an ecosystem of bots. However, our knowledge of how these automated agents are interacting with each other is rather poor. Bots are predictable automatons that do not have the capacity for emotions, meaning-making, creativity, and sociality and it is hence natural to expect interactions between bots to be relatively predictable and uneventful...
2017: PloS One
https://www.readbyqxmd.com/read/28231251/empirical-lessons-regarding-contraception-in-a-protracted-refugee-setting-a-descriptive-study-from-maela-camp-on-the-thai-myanmar-border-1996-2015
#4
Somjet Srikanok, Daniel M Parker, Amber L Parker, Tracey Lee, Aung Myat Min, Pranee Ontuwong, Saw Oo Tan, Supachai Sirinonthachai, Rose McGready
Conflict settings and refugee camps can be chaotic places, with large and rapid population movements, exacerbated public health problems, and ad hoc health services. Reproductive health care that includes family planning is of heightened importance in such settings, however, funding and resources tend to be constrained and geared towards acute health services such as trauma management and infectious disease containment. Here we report on the complexities and challenges of providing family planning in a post-emergency refugee setting, using the example of the largest refugee camp on the Thai-Myanmar border, in existence now for over 30 years...
2017: PloS One
https://www.readbyqxmd.com/read/28231180/evolution-of-the-maillard-reaction-in-glutamine-or-arginine-dextrinomaltose-model-systems
#5
Silvia Pastoriza, José Ángel Rufián-Henares, Belén García-Villanova, Eduardo Guerra-Hernández
Enteral formulas are foods designed for medical uses to feed patients who are unable to eat normally. They are prepared by mixing proteins, amino acids, carbohydrates and fats and submitted to sterilization. During thermal treatment, the Maillard reaction takes place through the reaction of animo acids with reducing sugars. Thus, although glutamine and arginine are usually added to improve the nutritional value of enteral formulas, their final concentration may vary. Thus, in the present paper the early, intermediate, and advanced states of the Maillard reaction were studied in model systems by measuring loss of free amino acids through the decrease of fluorescence intensity with o-phtaldialdehyde (OPA), 5-Hydroximethylfurfural (HMF), furfural, glucosylisomaltol, fluorescence, and absorbance at 420 nm...
December 7, 2016: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28230707/low-cost-3d-printing-orbital-implant-templates-in-secondary-orbital-reconstructions
#6
Alison B Callahan, Ashley A Campbell, Carisa Petris, Michael Kazim
PURPOSE: Despite its increasing use in craniofacial reconstructions, three-dimensional (3D) printing of customized orbital implants has not been widely adopted. Limitations include the cost of 3D printers able to print in a biocompatible material suitable for implantation in the orbit and the breadth of available implant materials. The authors report the technique of low-cost 3D printing of orbital implant templates used in complex, often secondary, orbital reconstructions. METHODS: A retrospective case series of 5 orbital reconstructions utilizing a technique of 3D printed orbital implant templates is presented...
February 22, 2017: Ophthalmic Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28230656/role-of-nonneutralizing-antibodies-in-vaccines-and-or-hiv-infected-individuals
#7
Luzia Mayr, Bin Su, Christiane Moog
PURPOSE OF REVIEW: Increased body of evidence gathered over time indicate that antibodies are capable of many inhibitory mechanisms, virus neutralization being just one of them. Nonneutralizing antibodyactivities interfering with HIV replication can also lead to a decrease in viral load and even in-vivo protection. RECENT FINDINGS: It was previously believed that neutralizing antibodies can achieve sterilizing protection mainly by using their neutralization capacities against the infecting virus directly at the portal of virus entry...
February 21, 2017: Current Opinion in HIV and AIDS
https://www.readbyqxmd.com/read/28230521/micromonospora-terminaliae-sp-nov-an-endophytic-actinobacterium-isolated-from-the-surface-sterilized-stem-of-the-medicinal-plant-terminalia-mucronata
#8
Onuma Kaewkla, Arinthip Thamchaipinet, Christopher Milton Mathew Franco
An endophytic actinobacterium, strain TMS7T, was isolated from the stem of a Thai medicinal plant collected from the grounds of the Phujong-Nayoa National park, Ubon Ratchathani province, Thailand. As a result of a polyphasic taxonomy study, this strain was identified as a member of the genus Micromonospora. This strain was a Gram-stain-positive, aerobic actinobacterium with well-developed substrate mycelium with hyphae forming a single microspore was non-motile. Stran TMS7T was identified according to its 16S rRNA gene sequence as a new member of the genus Micromonospora...
February 24, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28228319/optimizing-natural-fertility-a%C3%A2-committee-opinion
#9
(no author information available yet)
This Committee Opinion provides practitioners with suggestions for optimizing the likelihood of achieving pregnancy in couples/individuals attempting conception who have no evidence of infertility. This document replaces the document of the same name previously published in 2013, Fertil Steril 2013;100(3):631-7.
January 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28228282/complement-factor-h-inhibits-cd47-mediated-resolution-of-inflammation
#10
Bertrand Calippe, Sebastien Augustin, Fanny Beguier, Hugo Charles-Messance, Lucie Poupel, Jean-Baptiste Conart, Shulong J Hu, Sophie Lavalette, Alexandre Fauvet, Julie Rayes, Olivier Levy, William Raoul, Catherine Fitting, Thomas Denèfle, Matthew C Pickering, Claire Harris, Sylvie Jorieux, Patrick M Sullivan, José-Alain Sahel, Philippe Karoyan, Przemyslaw Sapieha, Xavier Guillonneau, Emmanuel L Gautier, Florian Sennlaub
Variants of the CFH gene, encoding complement factor H (CFH), show strong association with age-related macular degeneration (AMD), a major cause of blindness. Here, we used murine models of AMD to examine the contribution of CFH to disease etiology. Cfh deletion protected the mice from the pathogenic subretinal accumulation of mononuclear phagocytes (MP) that characterize AMD and showed accelerated resolution of inflammation. MP persistence arose secondary to binding of CFH to CD11b, which obstructed the homeostatic elimination of MPs from the subretinal space mediated by thrombospsondin-1 (TSP-1) activation of CD47...
February 21, 2017: Immunity
https://www.readbyqxmd.com/read/28227920/in-vitro-evaluation-of-the-long-term-stability-of-pedot-pss-coated-microelectrodes-for-chronic-recording-and-electrical-stimulation-of-neurons
#11
A Schander, T Tesmann, S Strokov, H Stemmann, A K Kreiter, W Lang, A Schander, T Tesmann, S Strokov, H Stemmann, A K Kreiter, W Lang, A K Kreiter, W Lang, A Schander, H Stemmann, S Strokov
For the chronic application of neural prostheses long-term stable microelectrodes for electrical stimulation are essential. In recent years many developments were done to investigate different appropriate materials for these electrodes. One of these materials is the electrical conductive polymer PEDOT, which has low impedance and high charge injection capacity. However, the long-term stability of this polymer is still unclear. Thus this paper reports on the in-vitro evaluation of the long-term stability of PEDOT coated gold microelectrodes...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226554/effects-of-a-non-thermal-plasma-needle-device-on-hpv-16-positive-cervical-cancer-cell-viability-in-vitro
#12
Hollie A Ryan, Johanna Neuber, Shutong Song, Stephen J Beebe, Chunqi Jiang, Hollie A Ryan, Johanna Neuber, Shutong Song, Stephen J Beebe, Chunqi Jiang, Shutong Song, Johanna Neuber, Chunqi Jiang, Stephen J Beebe, Hollie A Ryan
Non-thermal atmospheric pressure plasma has been demonstrated to inactivate a wide range of surface-dwelling pathogenic microorganisms including airborne viral particles, vegetative bacteria and bacterial spores. This shows the promise of plasma-based decontamination procedures for broad-spectrum sterilization and disinfection and promotes the in-depth and systematic study of how plasma treatment conditions relate to pathogen inactivation efficiency. A wide knowledge gap nonetheless exists regarding whether certain plasma parameters and exposure conditions can alter the resistance of virus-linked cancer cells to treatment...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226235/plant-mitochondrial-genomes-dynamics-and-mechanisms-of-mutation
#13
José M Gualberto, Kathleen J Newton
The large mitochondrial genomes of angiosperms are unusually dynamic because of recombination activities involving repeated sequences. These activities generate subgenomic forms and extensive genomic variation even within the same species. Such changes in genome structure are responsible for the rapid evolution of plant mitochondrial DNA and for the variants associated with cytoplasmic male sterility and abnormal growth phenotypes. Nuclear genes modulate these processes, and over the past decade, several of these genes have been identified...
February 9, 2017: Annual Review of Plant Biology
https://www.readbyqxmd.com/read/28225571/balancing-selection-maintains-sex-determining-alleles-in-multiple-locus-complementary-sex-determination
#14
Jerome J Weis, Paul J Ode, George E Heimpel
Hymenopteran species in which sex is determined through a haplo-diploid mechanism known as complementary sex determination (CSD) are vulnerable to a unique form of inbreeding depression. Diploids heterozygous at one or more CSD loci develop into females but diploids homozygous at all loci develop into diploid males, which are generally sterile or inviable. Species with multiple polymorphic CSD loci (ml-CSD) may have lower rates of diploid male production than species with a single CSD locus (sl-CSD), but it is not clear if polymorphism is consistently maintained at all loci...
February 22, 2017: Evolution; International Journal of Organic Evolution
https://www.readbyqxmd.com/read/28224696/can-contaminated-water-be-rendered-safe-for-nasal-saline-irrigations
#15
Allison G Ordemann, James K Stanford, Donna C Sullivan, J Mark Reed
OBJECTIVES/HYPOTHESIS: To compare sterile water to three methods of sterilization (carbon filtration, boiling, and ultraviolet [UV] light) for preparation of nasal saline irrigants free of bacterial and amebic contaminants. STUDY DESIGN: Bench-top translational research and cost comparison. METHODS: Sterile water was compared to common sterilization methods. Sterile water was contaminated with known concentrations of Staphylococcus aureus, Pseudomonas aeruginosa, Moraxella catarrhalis, Acinetobacter baumannii, Klebsiella pneumonia, Legionella pneumophila, and Naegleria fowleri...
February 22, 2017: Laryngoscope
https://www.readbyqxmd.com/read/28223498/attenuated-pfspz-vaccine-induces-strain-transcending-t-cells-and-durable-protection-against-heterologous-controlled-human-malaria-infection
#16
Kirsten E Lyke, Andrew S Ishizuka, Andrea A Berry, Sumana Chakravarty, Adam DeZure, Mary E Enama, Eric R James, Peter F Billingsley, Anusha Gunasekera, Anita Manoj, Minglin Li, Adam J Ruben, Tao Li, Abraham G Eappen, Richard E Stafford, Natasha Kc, Tooba Murshedkar, Floreliz H Mendoza, Ingelise J Gordon, Kathryn L Zephir, LaSonji A Holman, Sarah H Plummer, Cynthia S Hendel, Laura Novik, Pamela J M Costner, Jamie G Saunders, Nina M Berkowitz, Barbara J Flynn, Martha C Nason, Lindsay S Garver, Matthew B Laurens, Christopher V Plowe, Thomas L Richie, Barney S Graham, Mario Roederer, B Kim Lee Sim, Julie E Ledgerwood, Stephen L Hoffman, Robert A Seder
A live-attenuated malaria vaccine, Plasmodium falciparum sporozoite vaccine (PfSPZ Vaccine), confers sterile protection against controlled human malaria infection (CHMI) with Plasmodium falciparum (Pf) parasites homologous to the vaccine strain up to 14 mo after final vaccination. No injectable malaria vaccine has demonstrated long-term protection against CHMI using Pf parasites heterologous to the vaccine strain. Here, we conducted an open-label trial with PfSPZ Vaccine at a dose of 9.0 × 10(5) PfSPZ administered i...
February 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28223181/the-effect-of-salpingectomy-on-ovarian-function
#17
REVIEW
Alexander Kotlyar, Julian Gingold, Shirley Shue, Tommaso Falcone
OBJECTIVE: To review the literature concerning the effects of salpingectomy on ovarian function as assessed using measures of ovarian reserve and outcomes from in-vitro fertilization and spontaneous pregnancy cycles. BACKGROUND: Tubal surgery is performed for a variety of indications in gynecology. Salpingectomy is the most aggressive form of tubal surgery and may be performed for potential risk reduction for epithelial ovarian cancer, sterilization, ectopic pregnancy and as a method to enhance fertility in the setting of a hydrosalpinx...
February 18, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/28223035/effects-of-quinestrol-on-the-vocal-behavior-of-mice-during-courtship-interactions
#18
Yi Chen, Qianqian Su, Quansheng Liu
Vocalizations are a crucial part of courtship and mating in a wide variety of species. Mating behavior, including courtship calls, is modulated by sex steroid hormones. Male mice produce courtship ultrasonic vocalizations to attract females during heterosexual encounters. However, rare is the knowledge on whether vocal behavior of mice changes under sterilant treatment which will affect gonadal hormone levels. In the present study, we treat male mice with quinestrol, which interferes with the release of the gonadotropin-releasing hormone (GnRH) and has a significant anti-fertility effect in rodents...
February 18, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/28222903/modified-mrna-vaccines-protect-against-zika-virus-infection
#19
Justin M Richner, Sunny Himansu, Kimberly A Dowd, Scott L Butler, Vanessa Salazar, Julie M Fox, Justin G Julander, William W Tang, Sujan Shresta, Theodore C Pierson, Giuseppe Ciaramella, Michael S Diamond
The emergence of ZIKV infection has prompted a global effort to develop safe and effective vaccines. We engineered a lipid nanoparticle (LNP) encapsulated modified mRNA vaccine encoding wild-type or variant ZIKV structural genes and tested immunogenicity and protection in mice. Two doses of modified mRNA LNPs encoding prM-E genes that produced virus-like particles resulted in high neutralizing antibody titers (∼1/100,000) that protected against ZIKV infection and conferred sterilizing immunity. To offset a theoretical concern of ZIKV vaccines inducing antibodies that cross-react with the related dengue virus (DENV), we designed modified prM-E RNA encoding mutations destroying the conserved fusion-loop epitope in the E protein...
February 16, 2017: Cell
https://www.readbyqxmd.com/read/28222701/the-effect-of-deep-muscle-relaxation-on-the-force-required-during-latissimus-dorsi-dissection-for-breast-reconstructive-surgery-results-of-a-prospective-double-blinded-observational-pilot-study
#20
T Ledowski, A Goodwin-Walters, P Quinn, M Calvert
BACKGROUND: The use of neuromuscular blocking agents has previously been suggested to facilitate the dissection of the latissimus dorsi muscle during breast reconstructive surgery. The aim of this study was to quantify the influence of deep muscle relaxation on the force required to lift the latissimus dorsi muscle during flap preparation. METHODS: After ethics approval and written informed consent 15 patients scheduled for elective breast reconstruction with a latissimus dorsi pedicled flap (muscle flap, not myocutaneous flap) under general anaesthesia were prospectively included...
February 21, 2017: BMC Anesthesiology
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