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https://www.readbyqxmd.com/read/28535126/activated-charcoal-significantly-reduces-the-amount-of-colchicine-released-from-gloriosa-superba-in-simulated-gastric-and-intestinal-media
#1
Shukry Zawahir, Indika Gawarammana, Paul I Dargan, Mahfoudh Abdulghni, Andrew H Dawson
BACKGROUND: Poisoning with Gloriosa superba, a plant containing colchicine, is common in Sri Lanka. OBJECTIVES: This study was to estimate release of colchicine from 5 g of different parts of Gloriosa superba in simulated gastric and intestinal media, and examine the binding efficacy of activated charcoal (AC) to colchicine within this model. METHODS: A USP dissolution apparatus-II was used to prepare samples for analysis of colchicine using HPLC...
May 23, 2017: Clinical Toxicology
https://www.readbyqxmd.com/read/28515508/evaluating-sterility-of-single-dose-vials-on-an-automated-compounding-device
#2
Hannah M Hutchinson, Brian E Sayre, Terrence Prettyman, Erica King
Background: Current guidelines for sterile compounding require that single dose vials of pharmaceuticals must be discarded after 6 hours when accessed in an ISO Class 5 environment. At this time, no studies have evaluated the sterility of single dose vials at any time after opening. Objective: The purpose of this study is to evaluate the sterility of single dose vials attached to an automated compounding device for up to 24 hours and accessed and maintained within a cleanroom environment. Methods: This is a prospective, observational study evaluating the sterility of 32 pooled samples of manufactured single dose injectable drugs attached to an automated compounding device for up to 24 hours and maintained within an ISO Class 5 environment in an ISO Class 7 buffer area...
April 2017: Hospital Pharmacy
https://www.readbyqxmd.com/read/28510140/nuclear-receptors-ecr-usp-e75-dhr3-and-err-regulate-transcription-of-ecdysone-cascade-genes
#3
M Yu Mazina, E V Kocheryzhkina, J V Nikolenko, A N Krasnov, S G Georgieva, N E Vorobyeva
We found that an increase in the expression level of E75, DHR3, and ERR increases the degree of activation of dhr3 and hr4 genes in Drosophila S2 cells. We also detected a repressing effect of these nuclear receptors on the basal transcription level of these genes. This is the first study to show the ability of nuclear receptors E75, DHR3, and ERR to function as activators or repressors depending on external conditions. We also confirmed the existence of the interaction of all studied nuclear receptors with the promoters of dhr3 and hr4 genes of the ecdysone cascade in vivo...
March 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28506870/development-of-solid-sedds-vii-effect-of-pore-size-of-silica-on-drug-release-from-adsorbed-self-emulsifying-lipid-based-formulations
#4
Suhas G Gumaste, Abu T M Serajuddin
PURPOSE: Lipid-based self-emulsifying drug delivery systems (SEDDS) are usually liquids, and they can be converted into solid dosage forms by adsorbing onto silicates. However, most commercially available silicates are mesoporous with small pore sizes of 1 to 50nm that lead to incomplete emulsification of SEDDS inside the pores and thus incomplete drug release. The objective of this study was to investigate the impact of silica pore size on the extent of drug release from SEDDS solidified by adsorbing onto macroporous silicas with different pore sizes...
May 12, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28504132/non-destructive-and-direct-determination-of-the-degree-of-substitution-of-carboxymethyl-cellulose-by-hr-mas-13-c-nmr-spectroscopy
#5
M Ferro, F Castiglione, W Panzeri, R Dispenza, L Santini, H J Karlsson, P P de Wit, A Mele
We report on the direct assessment of the degree of substitution (DS) of carboxymethyl cellulose (CMC) by High Resolution Magic Angle Spinning (HR-MAS) (13)C NMR spectroscopy. The method is applied to industrial CMCs with low and high viscosity and nominal DS, purified and technical samples, and from cellulose linters or wood. The preparation of a set of purified CMC working standards with accurate DS values for the method validation is also described. The DS values determined via HR-MAS (13)C NMR on the industrial samples are critically compared to the corresponding values achieved through the USP 37 〈281〉 method (ASH method) and the HPLC method, and the advantages and limitations of the HR-MAS NMR method highlighted...
August 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28498552/ex-vivo-biomechanical-stability-of-5-cricoid-suture-constructs-for-equine-laryngoplasty
#6
Olivier Brandenberger, Fabrice Rossignol, Justin D Perkins, Antoine Lechartier, Céline Mespoulhès-Rivière, Amelie Vitte, Anthony Rossignol, Norm Ducharme, Karl Joseph Boening
OBJECTIVE: To determine the biomechanical properties of 5 suture constructs in the equine cricoid under cyclic loading and load to failure testing. STUDY DESIGN: Ex vivo study. SAMPLES: Seventy-five equine cadaver larynges. METHODS: Each larynx was implanted with 1 of 5 cricoid-suture constructs. The standard laryngoplasty, where a suture is passed once through the cricoid, including its caudal edge, was used in 2 constructs: 1 with 5 USP Ethibond (ES) and 1 with 2 mm Fibertape (FS)...
May 12, 2017: Veterinary Surgery: VS
https://www.readbyqxmd.com/read/28498477/identification-of-candidate-substrates-of-ubiquitin-specific-protease-13-using-2d-dige
#7
Jianmin Wang, Yingli Liu, Lijuan Tang, Sufen Qi, Yingjun Mi, Dianwu Liu, Qingbao Tian
The present study aimed to identify candidate substrates of ubiquitin-specific protease (USP)13 using two-dimensional fluorescence difference gel electrophoresis (2D-DIGE). USP13 is a well-characterized member of the USP family, which regulates diverse cellular functions by cleaving ubiquitin from ubiquitinated protein substrates. However, existing studies indicate that USP13 has no detectable hydrolytic activity in vitro. This finding implies that USP13 likely has different substrate specificity. In this study, a USP cleavage assay was performed using two different types of model substrates (glutathione S-transferase-Ub52 and ubiquitin-β-galactosidase) to detect the deubiquitinating enzyme (DUB) activity of USP13...
May 10, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28482514/optimization-of-two-biopolymer-based-oral-films-for-the-delivery-of-bioactive-molecules
#8
Pedro M Castro, Pedro Fonte, Ana Oliveira, Ana Raquel Madureira, Bruno Sarmento, Manuela E Pintado
An experimental design was established in order to optimize the mechanical properties of two oral film formulations intended for oral delivery of bioactive compounds. Carboxymethylcellulose (CMC) and gelatin type A (GelTA) were selected as polymeric matrix. Scanning electron microscopy revealed that caffeine crystals were homogeneously dispersed onto oral film matrix. Fourier-transform infrared analysis did not indicate formation of new chemical entities. USP modified dissolution assay revealed that GelTA was more effective in controlling caffeine release since maximum caffeine release (97...
July 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28479843/usp-800-adds-significant-safety-standards-facility-upgrades-needed-to-protect-employees-from-hazardous-drugs
#9
Bruce E Beans
The new USP standard for handling hazardous drugs (HDs) will require millions of dollars in capital outlays for facility and equipment upgrades and also requires in-depth assessments of each HD that facilities handle, significant workflow and work practice changes, and thorough staff training.
May 2017: P & T: a Peer-reviewed Journal for Formulary Management
https://www.readbyqxmd.com/read/28473807/fnrl-and-three-dnr-regulators-are-used-for-the-metabolic-adaptation-to-low-oxygen-tension-in-dinoroseobacter-shibae
#10
Matthias Ebert, Sebastian Laaß, Andrea Thürmer, Louisa Roselius, Denitsa Eckweiler, Rolf Daniel, Elisabeth Härtig, Dieter Jahn
The heterotrophic marine bacterium Dinoroseobacter shibae utilizes aerobic respiration and anaerobic denitrification supplemented with aerobic anoxygenic photosynthesis for energy generation. The aerobic to anaerobic transition is controlled by four Fnr/Crp family regulators in a unique cascade-type regulatory network. FnrL is utilizing an oxygen-sensitive Fe-S cluster for oxygen sensing. Active FnrL is inducing most operons encoding the denitrification machinery and the corresponding heme biosynthesis. Activation of gene expression of the high oxygen affinity cbb3-type and repression of the low affinity aa3-type cytochrome c oxidase is mediated by FnrL...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28468752/usp4-inhibits-smad4-monoubiquitination-and-promotes-activin-and-bmp-signaling
#11
Fangfang Zhou, Feng Xie, Ke Jin, Zhengkui Zhang, Marcello Clerici, Rui Gao, Maarten van Dinther, Titia K Sixma, Huizhe Huang, Long Zhang, Peter Ten Dijke
SMAD4 is a common intracellular effector for TGF-β family cytokines, but the mechanism by which its activity is dynamically regulated is unclear. We demonstrated that ubiquitin-specific protease (USP) 4 strongly induces activin/BMP signaling by removing the inhibitory monoubiquitination from SMAD4. This modification was triggered by the recruitment of the E3 ligase, SMURF2, to SMAD4 following ligand-induced regulatory (R)-SMAD-SMAD4 complex formation. Whereas the interaction of the negative regulator c-SKI inhibits SMAD4 monoubiquitination, the ligand stimulates the recruitment of SMURF2 to the c-SKI-SMAD2 complex and triggers c-SKI ubiquitination and degradation...
May 3, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28461266/in-vitro-release-testing-method-development-for-ophthalmic-ointments
#12
Quanying Bao, Jie Shen, Rajan Jog, Carmen Zhang, Bryan Newman, Yan Wang, Stephanie Choi, Diane J Burgess
It is essential as well as challenging to develop a reliable in vitro release testing method for determining whether differences in release profiles exist between qualitatively and quantitatively equivalent ophthalmic ointment formulations. There is a lack of regulatory guidance on in vitro release testing methods for ophthalmic formulations. Three different in vitro release testing methods 1) USP apparatus 4 with semisolid adapters; 2) USP apparatus 2 with enhancer cells; and 3) Franz diffusion cells were investigated...
April 29, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28460615/formulation-characterization-and-in-vitro-evaluation-of-fast-dissolving-tablets-containing-gliclazide-hydrotropic-solid-dispersions
#13
Jyotsana R Madan, Virendra J Kamate, Rajendra Awasthi, Kamal Dua
BACKGROUND: Low aqueous solubility is a major problem faced with new drug molecules. The purpose of this research was to provide a fast dissolving oral dosage form of gliclazide (GLZ) using the concept of mixed hydrotropy. METHODS: Solubility of GLZ was determined individually in sodium salicylate, nicotinamide, lactose, sodium acetate, urea, trisodium citrate and sodium benzoate. Highest solubility was obtained in 40% sodium benzoate solution. In order to decrease the individual hydrotrope amount, mixed hydrotropic agents were used...
April 26, 2017: Recent Patents on Drug Delivery & Formulation
https://www.readbyqxmd.com/read/28455415/genomic-enabled-prediction-in-maize-using-kernel-models-with-genotype-%C3%A3-environment-interaction
#14
Massaine Bandeira E Souza, Jaime Cuevas, Evellyn Giselly de Oliveira Couto, Paulino Pérez-Rodríguez, Diego Jarquín, Roberto Fritsche-Neto, Juan Burgueño, Jose Crossa
Multi-environment trials are routinely conducted in plant breeding to select candidates for the next selection cycle. In this study, we compare the prediction accuracy of four developed genomic-enabled prediction models: (1) single-environment, main genotypic effect model (SM), (2) multi-environment, main genotypic effects model (MM), (3) multi-environment, single variance G×E deviation model (MDs), and (4) multi-environment, environment-specific variance G×E deviation model (MDe). Each of these four models were fitted using two kernel methods: a linear kernel Genomic Best Linear Unbiased Predictor, GBLUP (GB), and a non-linear kernel Gaussian kernel (GK)...
April 28, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28452697/a-content-analysis-of-unique-selling-propositions-of-tobacco-print-ads
#15
Megan Johnson Shen, Smita C Banerjee, Kathryn Greene, Amanda Carpenter, Jamie S Ostroff
OBJECTIVES: We describe the unique selling propositions (USPs) (propositions used to convince customers to use a particular brand/product by focusing on the unique benefit) of print tobacco ads. METHODS: A qualitative content analysis was conducted of print tobacco ads (N = 171) selected from August 2012 to August 2013 for cigarettes, moist snuff, e-cigarettes, cigars, and snus to determine the content and themes of USPs for tobacco ads. RESULTS: Cigarette ad USP themes focused on portraying the product as attractive; moist snuff ads focused on portraying product as masculine; cigar ads focused on selling a "high end product;" and new and emerging tobacco products (e-cigarette, snus) focused on directly comparing these products to cigarettes...
March 1, 2017: American Journal of Health Behavior
https://www.readbyqxmd.com/read/28450206/development-of-a-discriminative-biphasic-in-vitro-dissolution-test-and-correlation-with-in-vivo-pharmacokinetic-studies-for-differently-formulated-racecadotril-granules
#16
Jia Deng, Sven Staufenbiel, Shilei Hao, Bochu Wang, Andriy Dashevskiy, Roland Bodmeier
The purpose of this study was to discriminate the release behavior from three differently formulated racecadotril (BCS II) granules and to establish an in vitro-in vivo correlation. Three granule formulations of the lipophilic drug were prepared with equivalent composition but prepared with different manufacturing processes (dry granulation, wet granulation with or without binder). In vitro release of the three granules was investigated using a biphasic dissolution system (phosphate buffer pH6.8 and octanol) and compared to the conventional single phase USP II dissolution test performed under sink and non-sink conditions...
April 25, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28444869/assessment-of-the-usage-of-biodegradable-polymeric-matrix-in-vaginal-devices-to-sustain-progesterone-release-in-cows
#17
José Rodrigo Valim Pimentel, Milton Maturana Filho, Lucio Cardozo-Filho, José Augusto M Agnelli, Jefter Nascimento, Ed Hoffmann Madureira
The usage of timed artificial insemination (TAI) at a low cost leading to better reproductive rates has been the aim of several research groups in the field. Usually during TAI protocols, sustained progesterone (P4 ) release devices are employed. Most devices are constituted of a nylon skeleton covered with a silicon layer with P4 . A device based on biopolymers was developed in order to reduce costs and decrease its environmental impact. In this study, we compared the kinetics of sustained progesterone release among devices manufactured with a polymeric blend made of polyhydroxybutyrate-valerate (PHBV) and poly-ε-caprolactone (PCL) (DISP) which were compared with DIB® (Internal Bovine Device) used as the control...
April 25, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28439742/exploration-of-the-binding-affinities-between-ecdysone-agonists-and-ecr-usp-by-docking-and-mm-pb-gbsa-approaches
#18
Xueping Hu, Jin Xie, Song Hu, Li Zhang, Yanhong Dong
Ecdysone receptor (EcR) is a significant target in the identification of new environmentally friendly pesticides. There are two types of ecdysone agonists: steroidal ecdysone agonists and dibenzoylhydrazines (DBHs). In this study, various modeling methods (homology modeling, molecular docking, MD simulation, binding free energy calculation, and per-residue binding free energy decomposition) were utilized to study the different binding mechanisms of two types of ecdysone agonists. Our theoretical results indicated that the relative binding potencies of DBHs can be ranked sufficiently accurately using the MOE docking method...
May 2017: Journal of Molecular Modeling
https://www.readbyqxmd.com/read/28431780/tolterodine-tartrate
#19
Gennady Ananchenko, Jasmina Novakovic
Tolterodine tartrate belongs to the family of muscarinic receptor antagonists and is indicated for the treatment of overactive urinary bladder syndrome. This chapter provides an overview of physical, analytical, and ADME profiles; highlights methods of chemical synthesis; and discusses stability of tolterodine as a free base and/or its l-tartrate salt in solution and in the solid state. The information presented in this chapter is based on the peer-reviewed literature, compendial reports (USP, EP), and authors' data...
2017: Profiles of Drug Substances, Excipients, and related Methodology
https://www.readbyqxmd.com/read/28422566/content-uniformity-testing-for-stratified-samples-via-parametric-tolerance-interval-testing
#20
Steven Novick, Buffy Hudson-Curtis
Historically in the biopharmaceutical setting, USP<905> has been used to establish that a batch of drug product has acceptable content uniformity. More recently, alternative approaches such as the two one-sided parametric tolerance interval test (PTI-TOST) have been proposed to establish content uniformity. Traditionally, the PTI-TOST is implemented as a sequential, two-tiered test, under the generally accepted assumption that the data are independently and identically distributed. Since the material is sequenced through the manufacturing process over a period of time, there are conceptually arguable locations within each batch, for instance: beginning, middle, and end...
April 19, 2017: Journal of Biopharmaceutical Statistics
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