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https://www.readbyqxmd.com/read/28707700/deleting-the-arntl-clock-gene-in-the-granular-layer-of-the-mouse-cerebellum-impact-on-the-molecular-circadian-clockwork
#1
Tenna Bering, Mikkel Bloss Carstensen, Martin Fredensborg Rath
The suprachiasmatic nucleus houses the central circadian clock and is characterized by the timely regulated expression of clock genes. However, neurons of the cerebellar cortex also contain a circadian oscillator with circadian expression of clock genes being controlled by the suprachiasmatic nucleus. It has been suggested that the cerebellar circadian oscillator is involved in food anticipation, but direct molecular evidence of the role of the circadian oscillator of the cerebellar cortex is currently unavailable...
July 14, 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28704264/extravascular-renal-denervation-ameliorates-juvenile-hypertension-and-renal-damage-resulting-from-experimental-hyperleptinemia-in-rats
#2
Nynke R Oosterhuis, Ramon Fernandes, Nuria Maicas, Sung Eun Bae, Joaquim Pombo, Hendrik Gremmels, Lucilla Poston, Jaap A Joles, Anne-Maj Samuelsson
BACKGROUND: Material obesity in rodents is associated with neonatal hyperleptinemia and hypertension of sympathetic origin in adult offspring. Previously, we reported that experimentally induced hyperleptinemia in rat pups results in adulthood hypertension. Here, we addressed the hypothesis that experimental neonatal hyperleptinemia, through renal nerve activation, adversely affects adult renal function. METHOD: Sprague-Dawley male and female pups were treated with neonatal leptin (3 mg/kg, intraperitoneal) or neonatal saline, twice daily from postnatal day 9-14...
July 12, 2017: Journal of Hypertension
https://www.readbyqxmd.com/read/28680568/combined-oral-administration-of-l-arginine-and-tetrahydrobiopterin-in-a-rat-model-of-pulmonary-arterial-hypertension
#3
C Schreiber, M S Eilenberg, A Panzenboeck, M P Winter, H Bergmeister, R Herzog, J Mascherbauer, I M Lang, D Bonderman
Alterations in the nitric oxide (NO) pathway play a major role in pulmonary arterial hypertension (PAH). L-arginine (LA) and tetrahydrobiopterin (BH4) are main substrates in the production of NO, which mediates pulmonary vasodilation. Administration of either LA or BH4 decrease pulmonary artery pressure (PAP). A combined administration of both may have synergistic effects in the therapy of PAH. In a telemetrically monitored model of unilateral pneumonectomy and monocrotaline-induced PAH, male Sprague-Dawley rats received either LA (300 mg/kg; n = 15), BH4 (20 mg/kg; n = 15), the combination of LA and BH4 (300 mg/kg, 20 mg/kg; n = 15), or vehicle (control group; n = 10) from day 28 after monocrotaline induction...
March 2017: Pulmonary Circulation
https://www.readbyqxmd.com/read/28676724/a-mie-resonant-antenna-with-high-sensitivity-for-force-and-strain-measurement
#4
Lingling Wu, Xiaoqing Xi, Bo Li, Ji Zhou
We demonstrate the experimental and simulated performances of a Mie resonant antenna for force sensing with high sensitivity for compressive force and strain measurements inside soft materials. The proposed sensor is compatible with biological specimens and has small dimensions. It comprises a pair of dielectric cubes and an elastic layer of silicone rubber. The applied force is determined by measuring the redshift of the operating frequency when a mechanical load applied. Both simulated and experimental results demonstrate that the relationship between the frequency shift of the sensor and the applied compressive load could be fitted well using a quadratic equation with a maximum fitting error less than 17%, which enables highly sensitive telemetric force measurements to be performed inside structures by observing the operating frequency shift...
July 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28675567/a-modulatory-role-of-the-rax-homeobox-gene-in-mature-pineal-gland-function-investigating-the-photoneuroendocrine-circadian-system-of-a-rax-conditional-knockout-mouse
#5
Kristian Rohde, Tenna Bering, Takahisa Furukawa, Martin Fredensborg Rath
The retinal and anterior neural fold homeobox gene (Rax) controls development of the eye and the forebrain. Postnatal expression of Rax in the brain is restricted to the pineal gland, a forebrain structure devoted to melatonin synthesis. The role of Rax in pineal function is unknown. In order to investigate the role of Rax in pineal function while circumventing forebrain abnormalities of the global Rax knockout, we generated an eye and pineal-specific Rax conditional knockout mouse. Deletion of Rax in the pineal gland did not affect morphology of the gland, suggesting that Rax is not essential for pineal gland development...
July 4, 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28659868/issues-in-continuous-24-h-core-body-temperature-monitoring-in-humans-using-an-ingestible-capsule-telemetric-sensor
#6
Cathriona R Monnard, Elie-Jacques Fares, Julie Calonne, Jennifer L Miles-Chan, Jean-Pierre Montani, Dominique Durrer, Yves Schutz, Abdul G Dulloo
BACKGROUND: There is increasing interest in the use of pill-sized ingestible capsule telemetric sensors for assessing core body temperature (Tc) as a potential indicator of variability in metabolic efficiency and thrifty metabolic traits. The aim of this study was to investigate the feasibility and accuracy of measuring Tc using the CorTemp(®) system. METHODS: Tc was measured over an average of 20 h in 27 human subjects, with measurements of energy expenditure made in the overnight fasted state at rest, during standardized low-intensity physical activity and after a 600 kcal mixed meal...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28658078/comparison-of-incremental-intermittent-and-time-trial-testing-in-age-group-swimmers
#7
Rodrigo Zacca, Rui Azevedo, Ricardo Peterson Silveira, João Paulo Vilas-Boas, David B Pyne, Flávio A de S Castro, Ricardo J Fernandes
The aim of this study was to compare physiological and biomechanical characteristics between an incremental intermittent test and a time trial protocol in age-group swimmers. 11 national level age-group swimmers (6 male and 5 female) performed a 7 x 200-m incremental intermittent protocol (until exhaustion; 30 s rest) and a 400-m test (T400) in front crawl on separate days. Cardiorespiratory variables were measured continuously using a telemetric portable gas analyzer. Swimming speed, stroke rate, stroke length and stroke index were assessed by video analysis...
June 22, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28650242/evaluation-of-a-telemetric-gastrointestinal-pill-for-continuous-monitoring-of-gastrointestinal-temperature-in-horses-at-rest-and-during-exercise
#8
Elisabeth-Lidwien J M M Verdegaal, Catherine Delesalle, Charles G B Caraguel, Louise E Folwell, Todd J McWhorter, Gordon S Howarth, Samantha H Franklin
OBJECTIVE To evaluate use of a telemetric gastrointestinal (GI) pill to continuously monitor GI temperature in horses at rest and during exercise and to compare time profiles of GI temperature and rectal temperature. ANIMALS 8 Standardbred horses. PROCEDURES Accuracy and precision of the GI pill and a rectal probe were determined in vitro by comparing temperature measurements with values obtained by a certified resistance temperature detector (RTD) in water baths at various temperatures (37°, 39°, and 41°C)...
July 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28646025/remodeling-of-repolarization-and-arrhythmia-susceptibility-in-a-myosin-binding-protein-c-knockout-mouse-model
#9
Amir Toib, Chen Zhang, Giulia Borghetti, Xiaoxiao Zhang, Markus Wallner, Yijun Yang, Constantine Troupes, Hajime Kubo, Thomas Sharp, Eric Feldsott, Remus M Berretta, Neil Zalavadia, Danielle Trappanese, Shavonn Harper, Polina Gross, Xiongwen Chen, Sadia Mohsin, Steven Houser
Hypertrophic cardiomyopathy (HCM) is one of the most common genetic cardiac diseases and amongst the leading causes of sudden cardiac death (SCD) in the young. The cellular mechanisms leading to SCD in HCM are not well known. Prolongation of the action potential duration (APD) is a common feature predisposing hypertrophied hearts to SCD. Previous studies have explored the roles of inward Na(+) and Ca(2+) in the development of HCM, but the role of repolarizing K(+) currents have not been defined. The objective of this study was to characterize the arrhythmogenic phenotype and cellular electrophysiological properties of mice with HCM, induced by Myosin Binding Protein C (MyBPC) Knockout (KO) and to test the hypothesis that remodeling of repolarizing K(+) currents cause APD prolongation in MyBPC KO myocytes...
June 23, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28645495/validity-and-reliability-of-the-mytemp-ingestible-temperature-capsule
#10
Coen C W G Bongers, Maria T E Hopman, Thijs M H Eijsvogels
OBJECTIVES: An accurate and non-invasive measurement of core body temperature (Tc) is of great importance to quantify exercise-induced increases in Tc in athletes or to assess changes in Tc in patient populations. The use of ingestible gastrointestinal telemetric temperature capsules is widely accepted as a surrogate marker for Tc, but widespread implementation is lacking due to the high costs of these disposable capsules. A new and cheaper temperature capsule system (i.e. myTemp) was recently introduced...
June 8, 2017: Journal of Science and Medicine in Sport
https://www.readbyqxmd.com/read/28641720/fenofibrate-attenuates-hypertension-in-goldblatt-hypertensive-rats-role-of-20-hydroxyeicosatetraenoic-acid-in-the-nonclipped-kidney
#11
Alexandra Sporková, Věra Čertíková Chábová, Šárka Doleželová, Šárka Jíchová, Libor Kopkan, Zdeňka Vaňourková, Elzbieta Kompanowska-Jezierska, Janusz Sadowski, Hana Maxová, Luděk Červenka
BACKGROUND: There is vast evidence that the renin-angiotensin system is not the sole determinant of blood pressure (BP) elevation in human renovascular hypertension or the relevant experimental models. This study tested the hypothesis that kidney deficiency of 20-hydroxyeicosatetraenoic acid (20-HETE), a product of cytochrome P450 (CYP)-dependent ω-hydroxylase pathway of arachidonic acid metabolism, is important in the pathophysiology of the maintenance phase of 2-kidney, 1-clip (2K1C) Goldblatt hypertension...
June 2017: American Journal of the Medical Sciences
https://www.readbyqxmd.com/read/28617378/-the-influence-of-growing-seizure-readiness-on-the-autonomic-regulation-of-the-heart
#12
M L Mamalyga, L M Mamalyga
AIM: To study the functional state of the heart in a progressive increase of seizure readiness (SR) due to pentylenetetrazole kindling. MATERIAL AND METHODS: The study was carried out on male rats of the Wistar line. Simultaneous telemetric monitoring of electrocardiogram and electroencephalogram on-line was used. Individual SR of animals in 7- and 27 day pentylenetetrazole kindling was determined. RESULTS: The decrease in the index of LF/HF after 7 days of kindling reflects a compensatory reaction of the autonomic nervous system...
2017: Zhurnal Nevrologii i Psikhiatrii Imeni S.S. Korsakova
https://www.readbyqxmd.com/read/28611608/coping-style-modifies-general-and-affective-autonomic-reactions-of-domestic-pigs-in-different-behavioral-contexts
#13
Annika Krause, Birger Puppe, Jan Langbein
Based on individual adaptive strategies (coping), animals may react differently to environmental challenges in terms of behavior and physiology according to their emotional perception. Emotional valence as well as arousal may be derived by measuring vagal and sympathetic tone of the autonomic nervous system (ANS). We investigated the situation-dependent autonomic response of 16 domestic pigs with either a reactive or a proactive coping style, previously selected according to the backtest which is accepted in piglets to assess escape behavior...
2017: Frontiers in Behavioral Neuroscience
https://www.readbyqxmd.com/read/28599667/multipolar-pacing-by-cardiac-resynchronization-therapy-with-a-defibrillators-treatment-in-type-2-diabetes-mellitus-failing-heart-patients-impact-on-responders-rate-and-clinical-outcomes
#14
Celestino Sardu, Michelangela Barbieri, Matteo Santamaria, Valerio Giordano, Cosimo Sacra, Pasquale Paolisso, Alessandro Spirito, Raffaele Marfella, Giuseppe Paolisso, Maria Rosaria Rizzo
BACKGROUND: Type 2 diabetes mellitus (T2DM) is a multi factorial disease, affecting clinical outcomes in failing heart patients treated by cardiac resynchronization therapy with a defibrillator (CRT-d). METHODS: One hundred and ninety-five T2DM patients received a CRT-d treatment. Randomly the study population received a CRT-d via multipolar left ventricle (LV) lead pacing (n 99, multipolar group), vs a CRT-d via bipolar LV pacing (n 96, bipolar group). These patients were followed by clinical, and instrumental assessment, and telemetric device control at follow up...
June 9, 2017: Cardiovascular Diabetology
https://www.readbyqxmd.com/read/28599579/forced-air-pre-warming-prevents-peri-anaesthetic-hypothermia-and-shortens-recovery-in-adult-rats
#15
C J Schuster, D S J Pang
General anaesthesia disrupts thermoregulation in mammals, which can cause hypothermia. Decreases in core body temperature of 1℃ cause significant postoperative complications in humans, and peri-anaesthetic hypothermia in mice increases data variability, which can potentially increase animal use. In rats, the impact of different temperature management strategies on the incidence and severity of hypothermia, and the accuracy of different temperature measurement methods, is unknown. Eighteen adult male and female SD rats were block-randomized to one of three treatment groups: no-warming (NW), limited-warming (LW, heat pad during anaesthesia), and pre-warming (PW, warm air exposure before anaesthesia, followed by heat pad)...
January 1, 2017: Laboratory Animals
https://www.readbyqxmd.com/read/28586937/telemetric-intracranial-pressure-recording-via-a-shunt-system-integrated-sensor-a-safety-and-feasibility-study
#16
Philipp Ertl, Elvis J Hermann, Hans E Heissler, Joachim K Krauss
No abstract text is available yet for this article.
June 6, 2017: Journal of Neurological Surgery. Part A, Central European Neurosurgery
https://www.readbyqxmd.com/read/28567504/a%C3%A2-clinical-example-of-extreme-dose-exposure-for-an-implanted-cardioverter-defibrillator-beyond-the-degro-guidelines
#17
Yoana Hristova, Janett Köhn, Stefanie Preuß, Claus Rödel, Panagiotis Balermpas
INTRODUCTION: Considering that the number of malignant diseases in patients over 65 years of age is increasing, it often occurs that patients who carry a cardiac implanted electronic device must undergo radiotherapy. Ionizing radiation can disturb the function of the implantable cardioverter-defibrillator (ICD). As a result of this, an update of the DEGRO/DKG guidelines for radiotherapy of this patient group has been published. METHODS: We report the case of a patient with an ICD and T‑lymphoblastic lymphoma with cardiac involvement, who received i...
May 31, 2017: Strahlentherapie und Onkologie: Organ der Deutschen Röntgengesellschaft ... [et Al]
https://www.readbyqxmd.com/read/28557670/validity-and-reliability-of-the-moxy-oxygen-monitor-during-incremental-cycling-exercise
#18
E M Crum, W J O'Connor, L Van Loo, M Valckx, S R Stannard
INTRODUCTION: The Moxy is a novel, cutaneously placed muscle oxygen monitor which claims to measure local oxygen saturation (SmO2) and total haemoglobin (THb) using near-infrared spectroscopy. If shown to be reliable, its data storage and telemetric capability will be useful for assessing localised O2 usage during field-based exercise. This study investigated the reliability of the Moxy during cycling and assessed the correlations between its measurements, whole-body O2 consumption (VO2) and heart rate (HR)...
May 30, 2017: European Journal of Sport Science
https://www.readbyqxmd.com/read/28548283/novel-selective-pde-type-1-inhibitors-cause-vasodilatation-and-lower-blood-pressure-in-rats
#19
Morten Laursen, Lilliana Beck, Jan Kehler, Claus Tornby Christoffersen, Christoffer Bundgaard, Susie Mogensen, Tomas Joachim Mow, Estéfano Pinilla, Jakob Schöllhammer Knudsen, Elise Røge Hedegaard, Morten Grunnet, Ulf Simonsen
BACKGROUND AND PURPOSE: The PDE enzymes (PDE1-11) hydrolyse and thus inactivate cyclic nucleotides and are important in the regulation of the cardiovascular system. Here,we have investigated the effects on the cardiovascular system, of two novel selective PDE1 inhibitors, Lu AF41228 and Lu AF58027. EXPERIMENTAL APPROACH: We used rat mesenteric small arteries (internal diameters of 200-300 μm), RT-PCR and measured isometric wall tension. Effects of Lu AF41228 and Lu AF58027 on heart rate and BP were assessed in both anaesthetized and conscious male rats...
August 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28542864/circadian-clustering-of-spontaneous-epileptic-seizures-emerges-after-pilocarpine-induced-status-epilepticus
#20
Julika Pitsch, Albert J Becker, Susanne Schoch, Johannes Alexander Müller, Marco de Curtis, Vadym Gnatkovsky
OBJECTIVE: Seizures in mesial temporal lobe epilepsy (MTLE) associated with hippocampal sclerosis are thought to develop with various latency intervals after an initial transient brain insult. To study seizure dynamics after an initial transient precipitating insult in a systematic fashion, we utilized continuous video-electroencephalography (EEG) monitoring after the induction of status epilepticus (SE) in a mouse MTLE model. METHODS: Continuous 24/7 video/telemetric hippocampal EEG recordings in the systemic pilocarpine MTLE mouse model...
May 24, 2017: Epilepsia
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