keyword
MENU ▼
Read by QxMD icon Read
search

citrate synthase

keyword
https://www.readbyqxmd.com/read/29351413/mitochondrial-content-is-preserved-throughout-disease-progression-in-the-mdx-mouse-model-of-duchenne-muscular-dystrophy-regardless-of-taurine-supplementation
#1
Robert G Barker, Victoria L Wyckelsma, Hongyang Xu, Robyn M Murphy
Mitochondrial dysfunction is a pathological feature of Duchenne muscular Dystrophy (DMD), a debilitating and fatal neuromuscular disorder characterised by progressive muscle wasting and weakness. Mitochondria are a source of cellular ATP and involved in Ca2+ regulation and apoptotic signalling. Ameliorating aberrant mitochondrial function has therapeutic potential for reducing DMD disease severity. The dystrophic mdx mouse exhibits peak muscle damage at 21-28d which stabilises after 8 weeks. The amino acid taurine is implicated in mitochondrial health and function, with endogenous concentrations low when measured during the cycle of peak muscle damage in mdx mice...
December 20, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/29350264/linking-muscle-metabolism-and-functional-variation-to-field-swimming-performance-in-bluegill-sunfish-lepomis-macrochirus
#2
David J Ellerby, Shauna Cyr, Angela X Han, Mika Lin, Lloyd A Trueblood
Skeletal muscle has diverse mechanical roles during locomotion. In swimming fish, power-producing muscles work in concert with the accessory muscles of the fins which augment and control power transfer to the water. Although fin muscles represent a significant proportion of the locomotor muscle mass, their physiological properties are poorly characterized. To examine the relationship between muscle metabolism and the differing mechanical demands placed on distinct muscle groups, we quantified the aerobic and glycolytic capacities of the myotomal, pectoral and caudal muscles of bluegill sunfish...
January 19, 2018: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
https://www.readbyqxmd.com/read/29334833/silver-nanoparticles-induce-neurotoxicity-in-a-human-embryonic-stem-cell-derived-neuron-and-astrocyte-network
#3
Neza Repar, Hao Li, Jose S Aguilar, Qingshun Quinn Li, Damjana Drobne, Yiling Hong
Silver nanoparticles (AgNPs) are among the most extensively used nanoparticles and are found in a variety of products. This ubiquity leads to inevitable exposure to these particles in everyday life. However, the effects of AgNPs on neuron and astrocyte networks are still largely unknown. In this study, we used neurons and astrocytes derived from human embryonic stem cells as a cellular model to study the neurotoxicity that is induced by citrate-coated AgNPs (AgSCs). Immunostaining with the astrocyte and neuron markers, glial fibrillary acidic protein and microtubule-associated protein-2 (MAP2), respectively, showed that exposure to AgSCs at the concentration of 0...
January 15, 2018: Nanotoxicology
https://www.readbyqxmd.com/read/29324867/comparative-transcriptome-analysis-reveals-key-genes-potentially-related-to-soluble-sugar-and-organic-acid-accumulation-in-watermelon
#4
Lei Gao, Shengjie Zhao, Xuqiang Lu, Nan He, Hongju Zhu, Junling Dou, Wenge Liu
Soluble sugars and organic acids are important components of fruit flavor and have a strong impact on the overall organoleptic quality of watermelon (Citrullus lanatus) fruit. Several studies have analyzed the expression levels of the genes related to soluble sugar accumulation and the dynamic changes in their content during watermelon fruit development and ripening. Nevertheless, to date, there have been no reports on the organic acid content in watermelon or the genes regulating their synthesis. In this study, the soluble sugars and organic acids in watermelon were measured and a comparative transcriptome analysis was performed to identify the key genes involved in the accumulation of these substances during fruit development and ripening...
2018: PloS One
https://www.readbyqxmd.com/read/29320819/molecular-and-biochemical-characterization-of-opisthorchis-viverrini-calreticulin
#5
Wanlapa Chaibangyang, Amornrat Geadkaew-Krenc, Suksiri Vichasri-Grams, Smarn Tesana, Rudi Grams
Calreticulin (CALR), a multifunctional protein thoroughly researched in mammals, comprises N-, P-, and C-domain and has roles in calcium homeostasis, chaperoning, clearance of apoptotic cells, cell adhesion, and also angiogenesis. In this study, the spatial and temporal expression patterns of the Opisthorchis viverrini CALR gene were analyzed, and calcium-binding and chaperoning properties of recombinant O. viverrini CALR (OvCALR) investigated. OvCALR mRNA was detected from the newly excysted juvenile to the mature parasite by RT-PCR while specific antibodies showed a wide distribution of the protein...
December 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/29318542/determination-of-aconitase-activity-a-substrate-of-the%C3%A2-mitochondrial-lon-protease
#6
Pedro M Quirós
Mitochondrial aconitase is a reversible enzyme that catalyzes the conversion of citrate to isocitrate in the tricarboxylic acid cycle. Mitochondrial aconitase is very sensitive to oxidative inactivation and can aggregate and accumulate in the mitochondrial matrix causing mitochondrial dysfunction. Lon protease, one of the major quality control proteases in mitochondria, degrades oxidized aconitase maintaining mitochondrial homeostasis. This chapter describes a step-by-step protocol for a simple and reliable measurement of mitochondrial aconitase, as well as citrate synthase activity, using isolated mitochondria from cells...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29316926/rna-guided-single-double-gene-repressions-in-corynebacterium-glutamicum-using-an-efficient-crispr-interference-and-its-application-to-industrial-strain
#7
Jaehyun Park, Hyojung Shin, Sun-Mi Lee, Youngsoon Um, Han Min Woo
BACKGROUND: The construction of microbial cell factories requires cost-effective and rapid strain development through metabolic engineering. Recently, RNA-guided CRISPR technologies have been developed for metabolic engineering of industrially-relevant host. RESULTS: To demonstrate the application of the CRISPR interference (CRISPRi), we developed two-plasmid CRISPRi vectors and applied the CRISPRi in Corynebacterium glutamicum to repress single target genes and double target genes simultaneously...
January 9, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29316265/effects-of-chronic-hypoxia-on-diaphragm-function-in-deer-mice-native-to-high-altitude
#8
Neal J Dawson, Sulayman A Lyons, Danielle A Henry, Graham R Scott
AIM: We examined the effects of chronic hypoxia on diaphragm function in high- and low-altitude populations of Peromyscus mice. METHODS: Deer mice (P. maniculatus) native to high altitude and congeneric mice native to low altitude (P. leucopus) were born and raised in captivity to adulthood, and were acclimated to normoxia or hypobaric hypoxia (12 or 9 kPa, simulating hypoxia at 4300 m and 7000 m) for 6-8 weeks. We then measured indices of mitochondrial respiration capacity, force production, and fatigue resistance in the diaphragm...
January 9, 2018: Acta Physiologica
https://www.readbyqxmd.com/read/29303599/proteomic-analysis-of-peroxynitrite-induced-protein-nitration-in-isolated-beef-heart-mitochondria
#9
M Kohutiar, A Eckhardt, I Mikšík, P Šantorová, J Wilhelm
Mitochondria are exposed to reactive nitrogen species under physiological conditions and even more under several pathologic states. In order to reveal the mechanism of these processes we studied the effects of peroxynitrite on isolated beef heart mitochondria in vitro. Peroxynitrite has the potential to nitrate protein tyrosine moieties, break the peptide bond, and eventually release the membrane proteins into the solution. All these effects were found in our experiments. Mitochondrial proteins were resolved by 2D electrophoresis and the protein nitration was detected by immunochemical methods and by nano LC-MS/MS...
January 5, 2018: Physiological Research
https://www.readbyqxmd.com/read/29288934/microplastics-have-a-more-profound-impact-than-elevated-temperatures-on-the-predatory-performance-digestion-and-energy-metabolism-of-an-amazonian-cichlid
#10
Bin Wen, Nan Zhang, Shi-Rong Jin, Zai-Zhong Chen, Jian-Zhong Gao, Ying Liu, Han-Peng Liu, Zhe Xu
Knowledge on the impacts of microplastics (MPs) pollution on freshwater environments and biota remains limited. Meanwhile, freshwater ecosystems have been threatened by elevated temperatures caused by climate change. To date, no information exists on how MPs-especially under elevated temperature conditions-affect predatory performance, digestive processes and metabolic pathways in freshwater organisms. Here, we examined MPs, elevated temperature and their combined effects on juveniles (0+ group) of an Amazonian cichlid, the discus fish (Symphysodon aequifasciatus)...
December 26, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/29280079/photobiomodulation-increases-mitochondrial-citrate-synthase-activity-in-rats-submitted-to-aerobic-training
#11
Wouber Hérickson de Brito Vieira, Cleber Ferraresi, Maria Luiza Barcellos Schwantes, Sérgio Eduardo de Andrade Perez, Vilmar Baldissera, Mikhail Santos Cerqueira, Nivaldo Antonio Parizotto
This study investigated the effects of photobiomodulation by low-laser laser therapy (LLLT) on the activities of citrate synthase (CS) and lactate dehydrogenase (LDH) and the anaerobic threshold (AT) in rats submitted to treadmill exercise. Fifty-four rats were allocated into four groups: rest control (RCG), rest laser (RLG), exercise control (ECG), and exercise laser (ELG). The infrared LLLT was applied daily on the quadriceps, gluteus maximum, soleus, and tibialis anterior muscles. Muscle samples (soleus, tibialis anterior, and cardiac muscles) were removed 48 h after the last exercise session for spectrophotometric analysis of the CS and LDH...
December 26, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/29258585/effects-of-endurance-training-on-skeletal-muscle-mitochondrial-function-in-huntington-disease-patients
#12
Sandro Manuel Mueller, Saskia Maria Gehrig, Jens A Petersen, Sebastian Frese, Violeta Mihaylova, Maria Ligon-Auer, Natalia Khmara, Jean-Marc Nuoffer, André Schaller, Carsten Lundby, Marco Toigo, Hans H Jung
BACKGROUND: Mitochondrial dysfunction may represent a pathogenic factor in Huntington disease (HD). Physical exercise leads to enhanced mitochondrial function in healthy participants. However, data on effects of physical exercise on HD skeletal muscle remains scarce. We aimed at investigating adaptations of the skeletal muscle mitochondria to endurance training in HD patients. METHODS: Thirteen HD patients and 11 healthy controls completed 26 weeks of endurance training...
December 19, 2017: Orphanet Journal of Rare Diseases
https://www.readbyqxmd.com/read/29250141/gynostemma-pentaphyllum-is-neuroprotective-in-a-rat-model-of-cardiopulmonary-resuscitation
#13
Gerburg Keilhoff, Torben Esser, Maximilian Titze, Uwe Ebmeyer, Lorenz Schild
Asphyxial cardiac arrest (ACA)-induced ischemia results in acute and delayed neuronal cell death. The early reperfusion phase is critical for the outcome. Intervention strategies directed to this period are promising to reduce ACA/resuscitation-dependent impairments. This study focused on the evaluation of the protective potential of an extract from Gynostemma pentaphyllum (GP), a plant used in traditional medicine with antioxidative, glucose lowering and neuroprotective activities, in an ACA rat model. We tested the following parameters: i) Basic systemic parameters such as pCO2 and blood glucose value within the first 30 min post-ACA; ii) mitochondrial response by determining activities of citrate synthase, respiratory chain complexes I + III and II + III, and the composition of cardiolipin 6 and 24 h post-ACA; iii) neuronal vitality of the CA1 hippocampal region by immunohistochemistry 24 h and 7 days post-ACA; and iv) cognitive function by a novel object recognition test 7 days post-ACA...
December 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29230719/resveratrol-and-or-exercise-training-counteract-aging-associated-decline-of-physical-endurance-in-aged-mice-targeting-mitochondrial-biogenesis-and-function
#14
Marwa Hassan Muhammad, Mona Maher Allam
Mitochondrial dysfunction and decreased mitochondrial content are hallmarks of aging that leads to decreased physical endurance. Our aim was to explore the anti-aging effect of resveratrol (RSVT) supplementation, a polyphenol, and/or exercise training, started at an older age, on improving physical activity, therefore, help in frailty avoidance and promotion of healthy aging in elderly. Eighteen-month-old aged mice received RSVT (15 mg/kg/day) and/or exercise trained for 4 weeks showed significant longer time to exhaustion with decreased blood lactate and free fatty acids levels associated with improved oxidative stress evidenced by decreased gastrocnemius muscle lipid peroxidation and increased antioxidant enzymes activities, catalase and superoxide dismutase, when compared to aged mice control group...
December 11, 2017: Journal of Physiological Sciences: JPS
https://www.readbyqxmd.com/read/29230177/effect-of-morinda-citrifolia-noni-enriched-diet-on-hepatic-heat-shock-protein-and-lipid-metabolism-related-genes-in-heat-stressed-broiler-chickens
#15
Joshua Flees, Hossein Rajaei-Sharifabadi, Elizabeth Greene, Lesleigh Beer, Billy M Hargis, Laura Ellestad, Tom Porter, Annie Donoghue, Walter G Bottje, Sami Dridi
Heat stress (HS) has been reported to alter fat deposition in broilers, however the underlying molecular mechanisms are not well-defined. The objectives of the current study were, therefore: (1) to determine the effects of acute (2 h) and chronic (3 weeks) HS on the expression of key molecular signatures involved in hepatic lipogenic and lipolytic programs, and (2) to assess if diet supplementation with dried Noni medicinal plant (0.2% of the diet) modulates these effects. Broilers (480 males, 1 d) were randomly assigned to 12 environmental chambers, subjected to two environmental conditions (heat stress, HS, 35°C vs...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29212609/effect-of-combined-exercise-versus-aerobic-only-training-on-skeletal-muscle-lipid-metabolism-in-a-rodent-model-of-type1-diabetes
#16
Michelle S Dotzert, Matthew W McDonald, Michael R Murray, J Zachary Nickels, Earl G Noble, C W James Melling
OBJECTIVES: Abnormal skeletal muscle lipid metabolism is associated with insulin resistance in people with type 1 diabetes. Although lipid metabolism is restored with aerobic exercise training, the risk for postexercise hypoglycemia is increased with this modality. Integrating resistance and aerobic exercise is associated with reduced hypoglycemic risk; however, the effects of this exercise modality on lipid metabolism and insulin resistance remain unknown. We compared the effects of combined (aerobic + resistance) versus aerobic exercise training on oxidative capacity and muscle lipid metabolism in a rat model of type 1 diabetes...
December 4, 2017: Canadian Journal of Diabetes
https://www.readbyqxmd.com/read/29212325/chronic-heat-stress-impairs-meat-quality-of-breast-muscle-in-broilers-through-affecting-redox-status-and-energy-substances-metabolism
#17
Zhuang Lu, Xiaofang He, Bingbing Ma, Lin Zhang, Jiaolong Li, Yun Jiang, Guanghong Zhou, Feng Gao
We investigated the molecular mechanisms of chronic heat stress impairing broilers breast meat quality. Broilers were assigned to three groups including normal control (NC) group, heat stress (HS) group and pair-fed (PF) group. After 7d of heat exposure (32°C), high temperature caused oxidative stress, and elevated the activity of citrate synthase (CS), mRNA expression of M-CPT1, and phosphorylation level of AMPKα, and reduced the mRNA expression of avUCP. After 14d of heat exposure, heat stress increased lightness and drip loss and decreased pH and shear force of breast meat...
December 7, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29211964/metabolic-costs-of-exposure-to-wastewater-effluent-lead-to-compensatory-adjustments-in-respiratory-physiology-in-bluegill-sunfish
#18
Sherry Du, Erin S McCallum, Maryam Vaseghi-Shanjani, Jasmine A Choi, Theresa R Warriner, Sigal Balshine, Graham R Scott
Municipal wastewater effluent is a major source of aquatic pollution and has potential to impact cellular energy metabolism. However, it is poorly understood whether wastewater exposure impacts whole-animal metabolism and whether this can be accommodated with adjustments in respiratory physiology. We caged bluegill sunfish (Lepomis macrochirus) for 21 days at two sites downstream (either 50 m or 830 m) from a wastewater treatment plant (WWTP). Survival was reduced in fish caged at both downstream sites compared to an uncontaminated reference site...
December 6, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29210997/tempol-supplementation-restores-diaphragm-force-and-metabolic-enzyme-activities-in-mdx-mice
#19
David P Burns, Izza Ali, Clement Rieux, James Healy, Greg Jasionek, Ken D O'Halloran
Duchenne muscular dystrophy (DMD) is characterized by striated muscle weakness, cardiomyopathy, and respiratory failure. Since oxidative stress is recognized as a secondary pathology in DMD, the efficacy of antioxidant intervention, using the superoxide scavenger tempol, was examined on functional and biochemical status of dystrophin-deficient diaphragm muscle. Diaphragm muscle function was assessed, ex vivo, in adult male wild-type and dystrophin-deficient mdx mice, with and without a 14-day antioxidant intervention...
December 6, 2017: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/29205418/transcriptional-regulation-of-acetyl-coa-and-lipid-synthesis-by-pii-protein-in-synechococcus-pcc-7942
#20
Ekta Verma, Sindhunath Chakraborty, Balkrishna Tiwari, Arun K Mishra
PII protein family is widespread in prokaryotes and plants. In this study, impacts of PII deficiency on the synthesis of acetyl CoA and acetyl CoA carboxylase enzyme (ACCase) was analyzed in the Synechococcus sp. PCC 7942 by evaluating the mRNA levels of pyruvate kinase (PK), pyruvate dehydrogenase (PDH), citrate synthase (CS), biotin synthase (BS), biotin carboxylase (BC), biotin carboxyl carrier protein (BCCP), carboxyl transferase (CT) α and β subunits. The PII deficient Synechococcus sp. PCC 7942 showed upregulation of all the above-mentioned genes, except CS...
December 5, 2017: Journal of Basic Microbiology
keyword
keyword
84975
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"