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citrate synthase

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https://www.readbyqxmd.com/read/28927427/first-molecular-detection-and-characterization-of-zoonotic-bartonella-species-in-fleas-infesting-domestic-animals-in-tunisia
#1
Saba Zouari, Fatma Khrouf, Youmna M'ghirbi, Ali Bouattour
BACKGROUND: Bartonellosis is an emerging vector-borne disease caused by different intracellular bacteria of the genus Bartonella (Rhizobiales: Bartonellaceae) that is transmitted primarily by blood-sucking arthropods such as sandflies, ticks and fleas. In Tunisia, there are no data available identifying the vectors of Bartonella spp. In our research, we used molecular methods to detect and characterize Bartonella species circulating in fleas collected from domestic animals in several of the country's bioclimatic areas...
September 19, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28925945/measurement-of-respiratory-chain-enzyme-activity-in-human-renal-biopsy-specimens
#2
Arun Ghose, Christopher M Taylor, Alexander J Howie, Anapurna Chalasani, Iain Hargreaves, David V Milford
Background: Mitochondrial disorders can present as kidney disease in children and be difficult to diagnose. Measurement of mitochondrial function in kidney tissue may help diagnosis. This study was to assess the feasibility of obtaining renal samples and analysing them for respiratory chain enzyme activity. Methods: The subjects were children undergoing a routine diagnostic renal biopsy, in whom a clinical condition of renal inflammation, scarring and primary metabolic disorder was unlikely. A fresh sample of kidney was snap frozen and later assayed for the activities of respiratory chain enzyme complexes I, II/III, and IV using spectrophotometric enzyme assay, and expressed as a ratio of citrate synthase activity...
September 19, 2017: Journal of Clinical Medicine
https://www.readbyqxmd.com/read/28923414/a-proteomic-approach-to-the-mechanisms-underlying-activation-of-aluminium-resistance-in-roots-of-urochloa-decumbens
#3
Catalina Arroyave, Roser Tolrà, Livia Chaves, Marcelo Claro de Souza, Juan Barceló, Charlotte Poschenrieder
The mechanisms of extreme Al-resistance in Urochloa decumbens are not established. Full resistance expression requires a lag time of 72-96h and is preceded by a sensitive phase (24-48h) with Al-induced root growth inhibition. The aim here was to identify key processes of the activation phase of Al-resistance analysing both root exudates and comparative root proteome. Samples were taken after 0, 24 and 96h exposure to 0 or 200μM Al. Al-induced stimulation of citrate and oxalate efflux was limited to the sensitive phase...
September 10, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28919865/coping-with-salt-water-habitats-metabolic-and-oxidative-responses-to-salt-intake-in-the-rufous-collared-sparrow
#4
Pablo Sabat, Cristóbal Narváez, Isaac Peña-Villalobos, Carolina Contreras, Karin Maldonado, Juan C Sanchez-Hernandez, Seth D Newsome, Roberto Nespolo, Francisco Bozinovic
Many physiological adjustments occur in response to salt intake in several marine taxa, which manifest at different scales from changes in the concentration of individual molecules to physical traits of whole organisms. Little is known about the influence of salinity on the distribution, physiological performance, and ecology of passerines; specifically, the impact of drinking water salinity on the oxidative status of birds has been largely ignored. In this study, we evaluated whether experimental variations in the salt intake of a widely-distributed passerine (Zontotrichia capensis) could generate differences in basal (BMR) and maximum metabolic rates (Msum), as well as affect metabolic enzyme activity and oxidative status...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28917145/cell-wall-pectin-methyl-esterification-and-organic-acids-of-root-tips-involve-in-aluminum-tolerance-in-camellia-sinensis
#5
Dongqin Li, Zaifa Shu, Xiaoli Ye, Jiaojiao Zhu, Junting Pan, Weidong Wang, Pinpin Chang, Chuanlei Cui, Jiazhi Shen, Wanping Fang, Xujun Zhu, Yuhua Wang
Tea plant (Camellia sinensis (O.) Kuntze) can survive from high levels of aluminum (Al) in strongly acidic soils. However, the mechanism driving its tolerance to Al, the predominant factor limiting plant growth in acid condition, is still not fully understood. Here, two-year-old rooted cuttings of C. sinensis cultivar 'Longjingchangye' were used for Al resistance experiments. We found that the tea plants grew better in the presence of 0.4 mM Al than those grew under lower concentration of Al treatments (0 and 0...
September 7, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28914762/the-1-week-and-8-month-effects-of-a-ketogenic-diet-or-ketone-salt-supplementation-on-multi-organ-markers-of-oxidative-stress-and-mitochondrial-function-in-rats
#6
Wesley C Kephart, Petey W Mumford, Xuansong Mao, Matthew A Romero, Hayden W Hyatt, Yufeng Zhang, Christopher B Mobley, John C Quindry, Kaelin C Young, Darren T Beck, Jeffrey S Martin, Danielle J McCullough, Dominic P D'Agostino, Ryan P Lowery, Jacob M Wilson, Andreas N Kavazis, Michael D Roberts
We determined the short- and long-term effects of a ketogenic diet (KD) or ketone salt (KS) supplementation on multi-organ oxidative stress and mitochondrial markers. For short-term feedings, 4 month-old male rats were provided isocaloric amounts of KD (n = 10), standard chow (SC) (n = 10) or SC + KS (~1.2 g/day, n = 10). For long-term feedings, 4 month-old male rats were provided KD (n = 8), SC (n = 7) or SC + KS (n = 7) for 8 months and rotarod tested every 2 months. Blood, brain (whole cortex), liver and gastrocnemius muscle were harvested from all rats for biochemical analyses...
September 15, 2017: Nutrients
https://www.readbyqxmd.com/read/28887840/pre-clinical-evaluation-of-n-acetylcysteine-reveals-side-effects-in-the-mdx-mouse-model-of-duchenne-muscular-dystrophy
#7
Gavin J Pinniger, Jessica R Terrill, Evanna B Assan, Miranda D Grounds, Peter G Arthur
Duchenne Muscular Dystrophy (DMD) is a fatal X-linked muscle wasting disease characterised by severe muscle weakness, necrosis, inflammation and oxidative stress. The antioxidant N-acetylcysteine (NAC) has been proposed as a potential therapeutic intervention for DMD boys. We investigated the capacity of NAC to improve dystrophic muscle function in the mdx mouse model of DMD. Young (6 w old) mdx and non-dystrophic C57 mice receiving 2% NAC in drinking water for 6 w were compared with untreated mice. Grip strength and body weight were measured weekly, before the 12 w old mice were anaesthetized and extensor digitorum longus (EDL) muscles excised for functional analysis and tissues sampled for biochemical analyses...
September 9, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28887308/uncovering-human-mettl12-as-a-mitochondrial-methyltransferase-that-modulates-citrate-synthase-activity-through-metabolite-sensitive-lysine-methylation
#8
Jędrzej Małecki, Magnus E Jakobsson, Angela Y Y Ho, Anders Moen, Arild Rustan, Pål Ø Falnes
Lysine methylation is an important and much-studied posttranslational modification of nuclear and cytosolic proteins, but is present also in mitochondria. However, the responsible mitochondrial lysine-specific methyltransferases (KMTs) remain largely elusive. Here, we investigated METTL12, a mitochondrial human S-adenosylmethionine (AdoMet)-dependent methyltransferase, and found it to methylate a single protein in mitochondrial extracts, identified as citrate synthase (CS). Using several in vitro and in vivo approaches, we demonstrated that METTL12 methylates CS on Lys-395, which is localized in the CS active site...
September 8, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28871187/prolonged-fasting-elicits-increased-hepatic-triglyceride-accumulation-in-rats-born-to-dexamethasone-treated-mothers
#9
Lucas Carminatti Pantaleão, Gilson Murata, Caio Jordão Teixeira, Tanyara Baliani Payolla, Junia Carolina Santos-Silva, Daniella Esteves Duque-Guimaraes, Frhancielly S Sodré, Camilo Lellis-Santos, Juliana Camargo Vieira, Dailson Nogueira de Souza, Patrícia Rodrigues Gomes, Sandra Campos Rodrigues, Gabriel Forato Anhe, Silvana Bordin
We investigated the effect of dexamethasone during the last week of pregnancy on glucose and lipid metabolism in male offspring. Twelve-week old offspring were evaluated after fasting for 12-hours (physiological) and 60-hours (prolonged). Physiological fasting resulted in glucose intolerance, decreased glucose clearance after pyruvate load and increased PEPCK expression in rats born to dexamethasone-treated mothers (DEX). Prolonged fasting resulted in increased glucose tolerance and increased glucose clearance after pyruvate load in DEX...
September 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28864949/skeletal-muscle-mitochondrial-mass-is-linked-to-lipid-and-metabolic-profile-in-individuals-with-spinal-cord-injury
#10
Laura C O'Brien, Qun Chen, Jeannie Savas, Edward J Lesnefsky, Ashraf S Gorgey
PURPOSE: Changes in metabolism and body composition after spinal cord injury (SCI) predispose individuals to obesity, type II diabetes, and cardiovascular disease. A link between lean mass and skeletal muscle mitochondrial mass has been reported but it is unknown how skeletal muscle mitochondrial mass and activity impact metabolic health. This study examined the relationship between skeletal muscle mitochondrial mass, activity and metabolic profile in individuals with chronic SCI. METHODS: Twenty-two men with motor complete SCI participated in the study...
September 1, 2017: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/28862604/-high-resolution-respirometry-in-diagnostic-of-mitochondrial-disorders-caused-by-mitochondrial-complex-i-deficiency
#11
T D Krylova, P G Tsygankova, Yu S Itkis, N L Sheremet, T A Nevinitsyna, S V Mikhaylova, E Yu Zakharova
Complex I (CI) deficiency is one of the most common defects in the OXPHOS system; it represents more than 30% cases of mitochondrial diseases. The group is characterized by clinical and genetic heterogeneity and comprise several nosological forms. The most prevalent phenotypes for CI are LHON and Leigh syndrome. In this study we have analyzed skin fibroblasts from 11 patients with mutations in mtDNA, which cause LHON or Leigh-like phenotypes: m.11778 G>A (n=3), m.3460 A>G (n=2), m.3635 G>A (n=1), m...
July 2017: Biomedit︠s︡inskai︠a︡ Khimii︠a︡
https://www.readbyqxmd.com/read/28857025/rickettsia-gravesii-sp-nov-a-novel-spotted-fever-group-rickettsia-in-western-australian-amblyomma-triguttatum-triguttatum-ticks
#12
Mohammad Y Abdad, Rita Abou Abdallah, Khalid El Karkouri, Mamadou Beye, John Stenos, Helen Owen, Nathan Unsworth, Ian Robertson, Stuart D Blacksell, Thi-Tien Nguyen, Claude Nappez, Didier Raoult, Stan Fenwick, Pierre-Edouard Fournier
A rickettsial organism harboured by Amblyomma triguttatum ticks on Barrow Island, Western Australia, was discovered after reports of possible rickettsiosis among local workers. Subsequent isolation of this rickettsia (strain BWI-1) in cell culture and analysis of its phylogenetic, genotypic and phenotypic relationships with type strains of Rickettsia species with standing in nomenclature suggested that it was sufficiently divergent to warrant its classification as a new species. Multiple gene comparison of strain BWI-1 revealed degrees of sequence similarity with Rickettsia raoultii, its closest relative, of 99...
September 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28855713/deciphering-clostridium-metabolism-and-its-responses-to-bioreactor-mass-transfer-during-syngas-fermentation
#13
Ni Wan, Ashik Sathish, Le You, Yinjie J Tang, Zhiyou Wen
This study used (13)C tracers and dynamic labeling to reveal metabolic features (nutrients requirements, pathway delineation and metabolite turnover rates) of Clostridium carboxidivorans P7, a model strain for industrial syngas fermentation, and its implication with bioreactor mass transfer. P7 shows poor activity for synthesizing amino acids (e.g., phenylalanine) and thus, needs rich medium for cell growth. The strain has multiple carbon fixation routes (Wood-Ljungdahl pathway, pyruvate:ferredoxin oxidoreductase reaction and anaplerotic pathways) and Re-citrate synthase (Ccar_06155) was a key enzyme in its tricarboxylic acid cycle (TCA) pathway...
August 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28855321/the-role-of-stochastic-thermal-environments-in-modulating-the-thermal-physiology-of-an-intertidal-limpet-lottia-digitalis
#14
Madeline J Drake, Nathan A Miller, Anne E Todgham
Much of our understanding of the thermal physiology of intertidal organisms comes from experiments with animals acclimated under constant conditions and exposed to a single heat stress. In nature, however, the thermal environment is more complex. Aerial exposure and the unpredictable nature of thermal stress during low tides may be critical factors in defining the thermal physiology of intertidal organisms. In the fingered limpet, Lottia digitalis, we investigated whether upper temperature tolerance and thermal sensitivity were influenced by the pattern of fluctuation with which thermal stress was applied...
September 1, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28852932/bioenergetic-variation-is-related-to-autism-symptomatology
#15
Leanna Delhey, Ekim Nur Kilinc, Li Yin, John Slattery, Marie Tippett, Rebecca Wynne, Shannon Rose, Stephen Kahler, Shirish Damle, Agustin Legido, Michael J Goldenthal, Richard E Frye
Autism spectrum disorder (ASD) has been associated with mitochondrial dysfunction but few studies have examined the relationship between mitochondrial function and ASD symptoms. We measured Complex I and IV and citrate synthase activities in 76 children with ASD who were not receiving vitamin supplementation or medication. We also measured language using the Preschool Language Scales or Clinical Evaluation of Language Fundamentals, adaptive behavior using the Vineland Adaptive Behavioral Scale, social function using the Social Responsiveness Scale and behavior using Aberrant Behavior Checklist, Childhood Behavior Checklist and the Ohio Autism Clinical Impression Scale...
August 29, 2017: Metabolic Brain Disease
https://www.readbyqxmd.com/read/28844961/mitochondrial-dysfunction-in-rat-splenocytes-following-hemorrhagic-shock
#16
Marie Warren, Kumar Subramani, Richard Schwartz, Raghavan Raju
The regulation of mitochondrial function is critical in cellular homeostasis following hemorrhagic shock. Hemorrhagic shock results in fluid loss and reduced availability of oxygen and nutrients to tissues. The spleen is a secondary lymphoid organ playing a key role in 'filtering the blood' and in the innate and adaptive immune responses. To understand the molecular basis of hemorrhagic shock, we investigated the changes in splenocyte mitochondrial respiration, and concomitant immune and metabolic alterations...
August 26, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28836276/the-expression-localization-and-function-of-%C3%AE-7-nicotinic-acetylcholine-receptor-in-rat-corpus
#17
Hedyeh Faghir-Ghanesefat, Nastaran Rahimi, Fatemeh Yarmohammadi, Tahmineh Mokhtari, Ali Reza Abdollahi, Shahram Ejtemaei Mehr, Ahmad R Dehpour
OBJECTIVES: Alpha7 nicotinic acetylcholine receptor (α7-nAChR), an emerging pharmacological target for a variety of medical conditions, is expressed in the most mammalian tissues with different effects. So, this study was designed to investigate the expression, localization and effect of α7-nAChR in rat corpus cavernosum (CC). METHODS & KEY FINDINGS: Reverse transcription polymerase chain reaction (RT-PCR) revealed that α7-nAChR was expressed in rat CC and double immunofluorescence studies demonstrated the presence of α7-nAChR in corporal neurons...
August 24, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28835707/fusion-or-fission-the-destiny-of-mitochondria-in-traumatic-brain-injury-of-different-severities
#18
Valentina Di Pietro, Giacomo Lazzarino, Angela Maria Amorini, Stefano Signoretti, Lisa J Hill, Edoardo Porto, Barbara Tavazzi, Giuseppe Lazzarino, Antonio Belli
Mitochondrial dynamics are regulated by a complex system of proteins representing the mitochondrial quality control (MQC). MQC balances antagonistic forces of fusion and fission determining mitochondrial and cell fates. In several neurological disorders, dysfunctional mitochondria show significant changes in gene and protein expression of the MQC and contribute to the pathophysiological mechanisms of cell damage. In this study, we evaluated the main gene and protein expression involved in the MQC in rats receiving traumatic brain injury (TBI) of different severities...
August 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28833381/proteomic-assessment-of-the-role-of-n-acyl-homoserine-lactone-in-shewanella-putrefaciens-spoilage
#19
Caili Zhang, Suqin Zhu, Abdul-Nabi Jatt, Yurong Pan, Mingyong Zeng
Shewanella spp. are the common spoilage organisms found in aquatic food products stored at low temperature and their spoilage mechanism has been reported to be mediated by quorum sensing (QS). However, the specifically expressed proteins responding to N-acyl homoserine-lactone (AHLs) were seldom reported. The present study aims to evaluate the effects of different AHL signal molecules on Shewanella putrefaciens Z4 isolated from refrigerated turbot (Scophthalmus maximus) at the proteome level. The results revealed that exogenous AHLs were utilized as QS signal molecules by S...
August 21, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28812165/methane-enhances-aluminum-resistance-in-alfalfa-seedlings-by-reducing-aluminum-accumulation-and-reestablishing-redox-homeostasis
#20
Weiti Cui, Hong Cao, Ping Yao, Jincheng Pan, Quan Gu, Sheng Xu, Ren Wang, Zhaozeng Ouyang, Qingya Wang, Wenbiao Shen
Methane (CH4) is emerging as a candidate of signal molecule recently. However, whether or how CH4 enhances plant adaptation to aluminum (Al)-contaminated environment is still unknown. In this report, the physiological roles and possible molecular mechanisms of CH4 in the modulation of Al toxicity in alfalfa seedlings were characterized. Our results showed that, CH4 pretreatment could alleviate Al-induced seedling growth inhibition and redox imbalance. The defensive effects of CH4 against Al toxicity including the remission of Al-induced root elongation inhibition, nutrient disorder, and relative electrolyte leakage...
August 15, 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
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