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https://www.readbyqxmd.com/read/28528488/enhanced-production-of-para-hydroxybenzoic-acid-by-genetically-engineered-saccharomyces-cerevisiae
#1
Nils J H Averesch, Alex Prima, Jens O Krömer
Saccharomyces cerevisiae is a popular organism for metabolic engineering; however, studies aiming at over-production of bio-replacement precursors for the chemical industry often fail to overcome proof-of-concept stage. When intending to show real industrial attractiveness, the challenge is twofold: formation of the target compound must be increased, while minimizing the formation of side and by-products to maximize titer, rate and yield. To tackle these, the metabolism of the organism, as well as the parameters of the process, need to be optimized...
May 20, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28528481/consent-an-event-or-a-memory-in-lumbar-spinal-surgery-a-multi-centre-multi-specialty-prospective-study-of-documentation-and-patient-recall-of-consent-content
#2
William B Lo, Ciaran P McAuley, Martin J Gillies, Patrick J Grover, Erlick A C Pereira
STUDY DESIGN: Prospective, multi-centre, multi-specialty medical notes review and patient interview. PURPOSE: The consenting process is an important communication tool which also carries medico-legal implications. While written consent is a pre-requisite before spinal surgery in the UK, the standard and effectiveness of the process have not been assessed previously. This study assesses standard of written consent for elective lumbar decompressive surgery for degenerative disc disease across different regions and specialties in the UK; level of patient recall of the consent content; and identifies factors which affect patient recall...
May 20, 2017: European Spine Journal
https://www.readbyqxmd.com/read/28528384/effects-of-salts-contained-in-lignocellulose-derived-sugar-streams-on-microbial-lipid-production
#3
Zhu Chen, Caixia Wan
This study aimed at developing low-cost, robust non-sterile fermentation processes for microbial lipid production from lignocellulose-derived sugars. Three representative oleaginous yeasts, Lipomyces tetrasporus (NRRL Y-11562), Rhodotorula toruloides (NRRL Y-1091), and Yarrowia lipolytica (NRRL YB-437), were tested for lipid production via non-sterile fermentation. Under optimal non-sterile conditions, all the tested strains had good performance on salt tolerance and lipid production. L. tetrasporus (NRRL Y-11562) gave the highest lipid titer of 12...
May 20, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28528382/optimal-production-of-a-rhodococcus-erythropolis-atcc-4277-biocatalyst-for-biodesulfurization-and-biodenitrogenation-applications
#4
Diego Todescato, Danielle Maass, Diego Alex Mayer, J Vladimir Oliveira, Débora de Oliveira, Selene M A Guelli Ulson de Souza, Antônio Augusto Ulson de Souza
Rhodococcus sp. has a broad catabolic diversity and unique enzymatic capabilities, and it is able to adapt under extreme conditions. Thereby, the production of this remarkable bacterium has a great biotechnological and industrial importance. In this sense, we sought to improve the R. erythropolis ATCC 4277 growth through a central composite design, by varying the components of nutrient medium (glucose, malt extract, yeast extract, CaCO3), temperature, and agitation. It was found that the concentrations of glucose and malt extract are not statistically significant, being reduced of 4...
May 20, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28528381/metal-chelate-affinity-precipitation-with-thermo-responsive-polymer-for-purification-of-%C3%AE%C2%B5-poly-l-lysine
#5
Sipeng Li, Zhaoyang Ding, Jifu Liu, Xuejun Cao
ε-Poly-L-lysine (ε-PL) is a natural preservative for food processing industry. A thermo-responsive polymer, attached with Cu(2+) or Ni(2+), was prepared for metal-chelate affinity precipitation for purification of ε-PL. The low critical solution temperatures (LCSTs) of these polymers were close to the room temperature (31.0-35.0 °C). The optimal adsorption conditions were as follows: pH 4.0, 0 mol/L NaCl, ligand density 75.00 μmol/g, and 120 min. The ligand Cu(2+) showed a stronger affinity interaction with ε-PL and the highest adsorption amount reached 251...
May 20, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28528283/poly-vinyl-alcohol-hydroxyapatite-monolithic-in-needle-extraction-mine-device-preparation-and-examination-of-drug-affinity
#6
Monika Pietrzyńska, Michał Czerwiński, Adam Voelkel
Polymer-ceramic materials based on poly(vinyl alcohol) (PVA) and hydroxyapatite were applied as sorption material in Monolithic In-Needle Extraction (MINE) device. The presented device provides new possibilities for the examination of bisphosphonates affinity for bone and will be a helpful tool in evaluation of potential antiresorptive drugs suitability. A ceramic part of monoliths was prepared by incorporation of hydroxyapatite (HA) into the reaction mixture or by using a soaking method (mineralization of HA on the PVA)...
May 17, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28527996/enzymatic-hydrolysis-of-pretreated-alfa-fibers-stipa-tenacissima-using-%C3%AE-d-glucosidase-and-xylanase-of-talaromyces-thermophilus-from-solid-state-fermentation
#7
Hanen Mallek-Fakhfakh, Jawhar Fakhfakh, Kamel Walha, Hajer Hassairi, Ali Gargouri, Hafedh Belghith
This work aims at realizing an optimal hydrolysis of pretreated Alfa fibers (Stipa tenacissima) through the use of enzymes produced from Talaromyces thermophilus AX4, namely β-D-glucosidase and xylanase, by a solid state fermentation process of an agro-industrial waste (wheat bran supplemented with lactose). The carbon source was firstly selected and the optimal values of three other parameters were determined: substrate loading (10g), moisture content (85%) and production time (10 days); which led to an optimized enzymatic juice...
May 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28527863/over-production-of-fermentable-sugar-for-bioethanol-production-from-carbohydrate-rich-malaysian-food-waste-via-sequential-acid-enzymatic-hydrolysis-pretreatment
#8
Halimatun Saadiah Hafid, Abdul Rahman Nor 'Aini, Mohd Noriznan Mokhtar, Ahmad Tarmezee Talib, Azhari Samsu Baharuddin, Md Shah Umi Kalsom
In Malaysia, the amount of food waste produced is estimated at approximately 70% of total municipal solid waste generated and characterised by high amount of carbohydrate polymers such as starch, cellulose, and sugars. Considering the beneficial organic fraction contained, its utilization as an alternative substrate specifically for bioethanol production has receiving more attention. However, the sustainable production of bioethanol from food waste is linked to the efficient pretreatment needed for higher production of fermentable sugar prior to fermentation...
May 17, 2017: Waste Management
https://www.readbyqxmd.com/read/28527738/life-cycle-assessment-of-sustainable-raw-material-acquisition-for-functional-magnetite-bionanoparticle-production
#9
Jhuma Sadhukhan, Nimisha Joshi, Mobolaji Shemfe, Jonathan R Lloyd
Magnetite nanoparticles (MNPs) have several applications, including use in medical diagnostics, renewable energy production and waste remediation. However, the processes for MNP production from analytical-grade materials are resource intensive and can be environmentally damaging. This work for the first time examines the life cycle assessment (LCA) of four MNP production cases: (i) industrial MNP production system; (ii) a state-of-the-art MNP biosynthesis system; (iii) an optimal MNP biosynthesis system and (iv) an MNP biosynthesis system using raw materials sourced from wastewaters, in order to recommend a sustainable raw material acquisition pathway for MNP synthesis...
May 17, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28527623/automated-gold-particle-quantification-of-immunogold-labeled-micrographs
#10
Rune Enger
BACKGROUND: Immunogold cytochemistry is the method of choice for precise localization of antigens on a subcellular scale. The process of immunogold quantification in electron micrographs is laborious, especially for proteins with a dense distribution pattern. NEW METHODS: Here I present a MATLAB based toolbox that is optimized for a typical immunogold analysis workflow. It combines automatic detection of gold particles through a multi-threshold algorithm with manual segmentation of cell membranes and regions of interests...
May 17, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28527581/nuclear-receptors-in-skeletal-homeostasis
#11
Hao Zuo, Yihong Wan
Nuclear receptors are a family of transcription factors that can be activated by lipophilic ligands. They are fundamental regulators of development, reproduction, and energy metabolism. In bone, nuclear receptors enable bone cells, including osteoblasts, osteoclasts, and osteocytes, to sense their dynamic microenvironment and maintain normal bone development and remodeling. Our views of the molecular mechanisms in this process have advanced greatly in the past decade. Drugs targeting nuclear receptors are widely used in the clinic for treating patients with bone disorders such as osteoporosis by modulating bone formation and resorption rates...
2017: Current Topics in Developmental Biology
https://www.readbyqxmd.com/read/28527515/optimization-of-critical-medium-components-for-enhancing-antibacterial-thiopeptide-nocathiacin-i-production-with-significantly-improved-quality
#12
Mao-Yu Yang, Jia-Wei Zhang, Xu-Ri Wu, Yi-Jun Chen
Nocathiacin I, a glycosylated thiopeptide antibiotic, displays excellent antibacterial activities against multidrug resistant bacterial pathogens. Previously, a novel nocathiacin I formulation for intravenous administration has been successfully developed and its aqueous solubility is greatly enhanced for clinical application. The purpose of the present study was to increase the fermentation titer of nocathiacin I and reduce or eliminate analogous impurities by screening the medium ingredients using response surface methodology...
April 2017: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/28527414/structural-characterization-and-discrimination-of-the-paris-polyphylla-var-yunnanensis-and-paris-vietnamensis-based-on-metabolite-profiling-analysis
#13
Li-Ping Kang, Yuan-Yuan Huang, Zhi-Lai Zhan, Da-Hui Liu, Hua-Sheng Peng, Tie-Gui Nan, Yuan Zhang, Qing-Xiu Hao, Jin-Fu Tang, Shou-Dong Zhu, Guang Yang, Lan-Ping Guo, Min Chen, Lu-Qi Huang
This study aimed to distinguish the rhizomes of Paris polyphylla var. yunnanensis (Franch) Hand Mazz (PPY) and Paris veitnamensis (Takht.) H. Li (PV) using metabolomics-based ultra high-performance liquid chromatography coupled with quadrupole time-of-fligh mass spectrometry (UHPLC/Q-TOF MS). First, the UHPLC/Q-TOF MS approach was optimized for metabolite profiling. Then, the MS data were processed using UNIFI™ combined with an in-house library to automatically characterize the metabolites. Based on the exact mass information, the fragmentation characteristics, and the retention time of compounds, and the fragmentation mechanism and retention behavior of steroidal glycosides in the references, the structures identified by UNIFI were further verified...
May 11, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28526652/retinoic-acid-induces-differentiation-of-buffalo-bubalus-bubalis-embryonic-stem-cells-into-germ-cells
#14
Syed Mohmad Shah, Suresh Kumar Singla, Prabhat Palta, Radhey Sham Manik, Manmohan Singh Chauhan
Development of precise and reproducible culture system for in vitro differentiation of embryonic stem (ES) cells into germ cells counts as a major leap forward for understanding not only the remarkable process of gametogenesis, otherwise obscured by limited availability of precursor primordial germ cells (PGCs), but in finally treating the catastrophic infertility. Taking into account the significant role of retinoic acid (RA) during in vivo gametogenesis, we designed the present study to investigate the effects of its stimulation on directing the differentiation of ES cells into germ cells...
May 16, 2017: Gene
https://www.readbyqxmd.com/read/28526295/large-scale-exploratory-genetic-analysis-of-cognitive-impairment-in-parkinson-s-disease
#15
Ignacio F Mata, Catherine O Johnson, James B Leverenz, Daniel Weintraub, John Q Trojanowski, Vivianna M Van Deerlin, Beate Ritz, Rebecca Rausch, Stewart A Factor, Cathy Wood-Siverio, Joseph F Quinn, Kathryn A Chung, Amie L Peterson-Hiller, Alberto J Espay, Fredy J Revilla, Johnna Devoto, Dora Yearout, Shu-Ching Hu, Brenna A Cholerton, Thomas J Montine, Karen L Edwards, Cyrus P Zabetian
Cognitive impairment is a common and disabling problem in Parkinson's disease (PD). Identification of genetic variants that influence the presence or severity of cognitive deficits in PD might provide a clearer understanding of the pathophysiology underlying this important nonmotor feature. We genotyped 1105 PD patients from the PD Cognitive Genetics Consortium for 249,336 variants using the NeuroX array. Participants underwent assessments of learning and memory (Hopkins Verbal Learning Test-Revised [HVLT-R]), working memory/executive function (Letter-Number Sequencing and Trail Making Test [TMT] A and B), language processing (semantic and phonemic verbal fluency), visuospatial abilities (Benton Judgment of Line Orientation [JoLO]), and global cognitive function (Montreal Cognitive Assessment)...
April 20, 2017: Neurobiology of Aging
https://www.readbyqxmd.com/read/28526212/3d-deeply-supervised-network-for-automated-segmentation-of-volumetric-medical-images
#16
Qi Dou, Lequan Yu, Hao Chen, Yueming Jin, Xin Yang, Jing Qin, Pheng-Ann Heng
While deep convolutional neural networks (CNNs) have achieved remarkable success in 2D medical image segmentation, it is still a difficult task for CNNs to segment important organs or structures from 3D medical images owing to several mutually affected challenges, including the complicated anatomical environments in volumetric images, optimization difficulties of 3D networks and inadequacy of training samples. In this paper, we present a novel and efficient 3D fully convolutional network equipped with a 3D deep supervision mechanism to comprehensively address these challenges; we call it 3D DSN...
May 8, 2017: Medical Image Analysis
https://www.readbyqxmd.com/read/28525957/determining-dominant-driving-forces-affecting-controlled-protein-release-from-polymeric-nanoparticles
#17
Josh Smith, Kayla G Sprenger, Rick Liao, Andrea Joseph, Elizabeth Nance, Jim Pfaendtner
Enzymes play a critical role in many applications in biology and medicine as potential therapeutics. One specific area of interest is enzyme encapsulation in polymer nanostructures, which have applications in drug delivery and catalysis. A detailed understanding of the mechanisms governing protein/polymer interactions is crucial for optimizing the performance of these complex systems for different applications. Using a combined computational and experimental approach, this study aims to quantify the relative importance of molecular and mesoscale driving forces to protein release from polymeric nanoparticles...
May 19, 2017: Biointerphases
https://www.readbyqxmd.com/read/28525882/interfacially-crosslinked-composite-porous-membranes-for-ultrafast-removal-of-anionic-dyes-from-water-through-permeating-adsorption
#18
Cuijing Liu, Liang Cheng, Yifan Zhao, Liping Zhu
The dye wastewater is one of the most difficult industrial wastewaters to treat. It keeps a big challenge to realize fast removal of dyes from water by membrane filtration due to the trade-off between separation selectivity and permeation flux for ultrafiltration or nanofiltration (NF) process. Here we report novel composite porous membranes which can remove anionic dyes from water by ultrafast permeating adsorption. A crosslinked polyethyleneimine (PEI) polymer with strong adsorption ability was incorporated onto a nylon microfiltration membrane by the interfacial amidation reaction between PEI and trimesoyl chloride...
April 12, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28525809/one-step-preparation-of-nanostructured-martite-catalyst-and-graphite-electrode-by-glow-discharge-plasma-for-heterogeneous-electro-fenton-like-process
#19
Alireza Khataee, Saeed Sajjadi, Aliyeh Hasanzadeh, Behrouz Vahid, Sang Woo Joo
Natural Martite ore particles and graphite were modified by alternating current (AC) glow discharge plasma to form nanostructured catalyst and cathode electrode for using in the heterogeneous-electro Fenton-like (Het-EF-like) process. The performance of the plasma-treated martite (PTM) and graphite electrode (PTGE) was studied for the treatment of paraquat herbicide in a batch system. 85.78% degradation efficiency for 20 mg L(-1) paraquat was achieved in the modified process under desired operational conditions (i...
May 16, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28525494/optimal-entry-point-and-trajectory-for-anterior-c1-lateral-mass-screw
#20
Yong Hu, Wei-Xin Dong, William Ryan Spiker, Zhen-Shan Yuan, Xiao-Yang Sun, Jiao Zhang, Hui Xie, Todd J Albert
STUDY DESIGN: A radiographic analysis of the anatomy of the C1 lateral mass using computed tomography (CT) scans and Mimics software. OBJECTIVE: To define the anatomy of the C1 lateral mass and make recommendations for optimal entry point and trajectory for anterior C1 lateral mass screws. SUMMARY OF BACKGROUND DATA: Although various posterior insertion angles and entry points for screw insertion have been proposed for posterior C1 lateral mass screws, no large series have been performed to assess the ideal entry point and optimal trajectory for anterior C1 lateral mass screw placement...
June 2017: Clinical Spine Surgery
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