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https://www.readbyqxmd.com/read/28433903/one-step-production-of-biodiesel-from-wet-and-unbroken-microalgae-biomass-using-deep-eutectic-solvent
#1
Ying Pan, Md Asraful Alam, Zhongming Wang, Dalong Huang, Keqin Hu, Hongxuan Chen, Zhenhong Yuan
One-step and Two-step methods were studied for lipid extraction from wet and unbroken (water content is 65-67%) Chlorella sp. and Chlorococcum sp. (GN38) using deep eutectic solvent (DES) treated microalgae cells. Further we optimized the extraction process and studied on its underlying mechanism. Among all DES, Choline chloride-Acetic acid (Ch-Aa) DES treatment showed optimal conditions at the mass ratio of DES: methanol-H2SO4 (2.00%) mixture: algae biomass was 60:40:3 with reaction time was 60min, and the optimum temperature was 110°C (Chlorococcum sp...
April 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28433902/evaluation-of-different-types-of-anaerobic-seed-sludge-for-the-high-rate-anaerobic-digestion-of-pig-slurry-in-uasb-reactors
#2
Carlos Rico, Jesús A Montes, José Luis Rico
Three different types of anaerobic sludge (granular, thickened digestate and anaerobic sewage) were evaluated as seed inoculum sources for the high rate anaerobic digestion of pig slurry in UASB reactors. Granular sludge performance was optimal, allowing a high efficiency process yielding a volumetric methane production rate of 4.1LCH4L(-1)d(-1) at 1.5days HRT (0.248LCH4g(-1)COD) at an organic loading rate of 16.4gCODL(-1)d(-1). The thickened digestate sludge experimented flotation problems, thus resulting inappropriate for the UASB process...
April 7, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28433874/systematically-characterize-the-absorbed-effective-substances-of-wutou-decoction-and-their-metabolic-pathways-in-rat-plasma-using-uhplc-q-tof-ms-combined-with-a-target-network-pharmacological-analysis
#3
Tengfei Xu, Shizhe Li, Yufei Sun, Zifeng Pi, Shu Liu, Fengrui Song, Zhiqiang Liu
Wu-tou Decoction (WTD) is a famous traditional Chinese medicine (TCM) formula which is applied to treat arthritis and pain of joints. In this study, a sensitive and rapid method was developed for the separation and identification of the absorbed constituents and metabolites of WTD in the rats plasma by ultra high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS). A total of 22 absorbed prototype constituents and 49 metabolites were identified or tentatively characterized in dosed plasma...
April 13, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28433678/montreal-accord-on-patient-reported-outcomes-use-series-paper-4-patient-reported-outcomes-pro-can-inform-clinical-decision-making-in-chronic-care
#4
Clifton O Bingham, Vanessa Noonan, Claudine Auger, Debbie Feldman, Sara Ahmed, Susan J Bartlett
BACKGROUND: Providing patient-centered healthcare requires that patient needs, preferences, and valued outcomes are more fully integrated into all decisions. Patient reported outcome measures (PROs) provide unique information from the patient perspective on overall health, symptoms, burden, and treatment response. OBJECTIVE: We sought to describe applications of PROs in clinical settings and considerations for implementation from the perspectives of PRO researchers, clinicians, administrators, policy makers, and patients attending a multidisciplinary meeting...
April 19, 2017: Journal of Clinical Epidemiology
https://www.readbyqxmd.com/read/28433639/optimization-of-atrazine-and-imidacloprid-removal-from-water-using-biochars-designing-single-or-multi-staged-batch-adsorption-systems
#5
Abhishek Mandal, Neera Singh
BACKGROUND: Contamination of surface and ground water by pesticides from agricultural runoff and industrial discharge is one of the main causes of aqueous contaminations world over. Biochar, agricultural waste derived highly aromatic substance produced after pyrolysis and carbonification of biomass have exhibited good adsorption capacity for pesticides and can be used to develop on-site bio-purification systems for organic contaminant removal from polluted waters. However, high amounts of adsorbent required in single stage-batch sorption plant increases the cost of water treatment; therefore, multistage plant systems were investigated...
April 5, 2017: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/28433344/application-of-a-rapid-hilic-uv-method-for-synthesis-optimization-and-stability-studies-of-immunogenic-neo-glycoconjugates
#6
F Rinaldi, S Tengattini, E Calleri, T Bavaro, L Piubelli, L Pollegioni, G Massolini, C Temporini
Proteins and glycoproteins with therapeutic activity are susceptible to environmental factors, which can cause their degradation and the loss of their activity. Thus, the maintenance of their stability during the production process is a critical factor. In this work, a simple and rapid hydrophilic interaction liquid chromatography HILIC-UV method was validated in terms of accuracy, precision, linearity, LOD, LOQ and specificity and applied to the investigation of the stability of intact proteins and their neo-glycoconjugates with antigenic activity against tuberculosis...
March 28, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28433265/high-pressure-microscopy-for-tracking-dynamic-properties-of-molecular-machines
#7
Masayoshi Nishiyama
High-pressure microscopy is one of the powerful techniques to visualize the effects of hydrostatic pressures on research targets. It could be used for monitoring the pressure-induced changes in the structure and function of molecular machines in vitro and in vivo. This review focuses on the dynamic properties of the assemblies and machines, analyzed by means of high-pressure microscopy measurement. We developed a high-pressure microscope that is optimized both for the best image formation and for the stability to hydrostatic pressure up to 150 MPa...
March 30, 2017: Biophysical Chemistry
https://www.readbyqxmd.com/read/28433238/pulmonary-rehabilitation-referral-and-participation-are-commonly-influenced-by-environment-knowledge-and-beliefs-about-consequences-a-systematic-review-using-the-theoretical-domains-framework
#8
Narelle S Cox, Cristino C Oliveira, Aroub Lahham, Anne E Holland
QUESTION: What are the barriers and enablers of referral, uptake, attendance and completion of pulmonary rehabilitation for people with chronic obstructive pulmonary disease (COPD)? DESIGN: Systematic review of qualitative or quantitative studies reporting data relating to referral, uptake, attendance and/or completion in pulmonary rehabilitation. PARTICIPANTS: People aged >18years with a diagnosis of COPD and/or their healthcare professionals...
April 2017: Journal of Physiotherapy
https://www.readbyqxmd.com/read/28433191/enzymatic-synthesis-of-isomaltotriose-palmitate-and-evaluation-of-its-emulsifying-property
#9
Kuan-Ju Liu
Enzymatic syntheses of oligosaccharide fatty acid esters are important owing to their wide range of industrial applications in the food, cosmetic, and pharmaceutical industries. Transesterification of isomaltotriose and palmitic acid vinyl ester, catalyzed by the metalloprotease thermolysin, was performed in organic solvents. The process parameters (reaction time and temperature) were optimized to achieve the highest yield of isomaltotriose palmitate (IP). The water content of the reaction system played a key role in the acylation of isomaltotriose...
June 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28433186/characterization-and-thermal-inactivation-kinetics-of-highly-thermostable-ramie-leaf-%C3%AE-amylase
#10
Liqin He, Sung-Hoon Park, Nguyen Dang Hai Dang, Hoa Xo Duong, Thi Phung Cac Duong, Phuong Lan Tran, Jong-Tae Park, Li Ni, Kwan-Hwa Park
We characterized ramie leaf β-amylase, and determined its thermostability and kinetic parameters. The enzyme was purified 53-fold using ammonium sulfate fractionation (40-60% saturation), anion exchange chromatography on DEAE-cellulose and gel permeation chromatography on Superdex-200. The purified enzyme was identified as β-amylase with molecular mass of 42kD. The enzyme displayed Km and kcat values for soluble potato starch of 1.1mg/mL and 7.8s(-1), respectively. The enzyme had a temperature optimum of 65°C, and its activity at 70°C was 92% of that at the optimal temperature after a 15-min incubation...
June 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28433077/development-and-clinical-utility-of-a-blood-based-test-service-for-the-rapid-identification-of-actionable-mutations-in-non-small-cell-lung-carcinoma
#11
Hestia Mellert, Trudi Foreman, Leisa Jackson, Dianna Maar, Scott Thurston, Kristina Koch, Amanda Weaver, Samantha Cooper, Nicholas Dupuis, Ubaradka G Sathyanarayana, Jakkie Greer, Westen Hahn, Dawne Shelton, Paula Stonemetz, Gary A Pestano
Nearly 80% of cancer patients do not have genetic mutation results available at initial oncology consultation; up to 25% of patients begin treatment before receiving their results. These factors hinder the ability to pursue optimal treatment strategies. This study validates a blood-based genome-testing service that provides accurate results within 72 hours. We focused on targetable variants in advanced non-small cell lung carcinoma-epidermal growth factor receptor gene (EGFR) variant L858R, exon 19 deletion (ΔE746-A750), and T790M; GTPase Kirsten ras gene (KRAS) variants G12C/D/V; and echinoderm microtubule associated protein like and 4 anaplastic lymphoma receptor tyrosine kinase fusion (EML4-ALK) transcripts 1/2/3...
May 2017: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/28433062/electrospun-nafion-polyacrylonitrile-nanofibers-as-an-ion-exchange-ultrathin-layer-chromatographic-stationary-phase
#12
Yanhui Wang, Susan V Olesik
An ion-exchange method to separate charged biomolecules on ultrathin layer chromatographic (UTLC) plates using electrospun Nafion-Polyacrylonitrile (PAN) nanofibers as the stationary phase is described. Sulfonate groups on Nafion provide the ion-exchange sites. The addition of PAN (a higher molecular weight polymer than Nafion) was used to facilitate the nanofiber formation process using electrospinning. Electrospinning parameters and separation conditions were optimized using fractional factorial design and response surface methodology...
June 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28433015/probing-the-effects-of-surface-hydrophobicity-and-tether-orientation-on-antibody-antigen-binding
#13
Derek B Bush, Thomas A Knotts
Antibody microarrays have the potential to revolutionize molecular detection for many applications, but their current use is limited by poor reliability, and efforts to change this have not yielded fruitful results. One difficulty which limits the rational engineering of next-generation devices is that little is known, at the molecular level, about the antibody-antigen binding process near solid surfaces. Atomic-level structural information is scant because typical experimental techniques (X-ray crystallography and NMR) cannot be used to image proteins bound to surfaces...
April 21, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28432951/microbial-communities-in-the-functional-areas-of-a-biofilm-reactor-with-anaerobic-aerobic-process-for-oily-wastewater-treatment
#14
Jianhua Li, Shanshan Sun, Ping Yan, Li Fang, Yang Yu, Yangdong Xiang, Di Wang, Yejing Gong, Yanjun Gong, Zhongzhi Zhang
Microbial communities in the functional areas of biofilm reactors with large height-diameter ratio using the anaerobic-aerobic (A/O) reflux process was investigated to treat heavy oil refinery wastewater without pretreatment. In the process, chemical oxygen demand (COD) and total nitrogen (TN) removal reached 93.2% and 82.8%, and the anaerobic biofilm reactor was responsible for 95% and 99%, respectively. Areas for hydrolysis acidification and acetic acid production, methane production, and COD recovery were obvious in the anaerobic reactor...
April 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28432922/restricted-access-magnetic-core-mesoporous-shell-microspheres-with-c8-modified-interior-pore-walls-for-the-identification-of-20-s-protopanaxadiol-metabolites-in-rat-plasma-using-uplc-q-tof-ms-ms
#15
Jin Ling, Yingjia Yu, Jianan Feng, Changjiang Xu, Jiebing Jiang, Liping Wang, Jiakun Long, Yan Li, Gengli Duan
In the present study, a novel sample preparation method based on magnetic core-mesoporous shell microspheres with C8-modified interior pore walls (C8-Fe3O4@mSiO2) was established for the identification of 20(S)-protopanaxadiol (PPD) metabolites in rat plasma by UPLC-Q-TOF-MS/MS analysis. C8-Fe3O4@mSiO2 allowed selective extraction of PPD metabolites from rat plasma by excluding macromolecules in the plasma owing to size exclusion effect. Five extraction conditions including the amount of C8-Fe3O4@mSiO2 microspheres used, extraction time, elution solvents, elution volume, and elution time were investigated and optimized...
April 3, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28432850/a-detailed-experimental-study-of-a-dna-computer-with-two-endonucleases
#16
Sebastian Sakowski, Tadeusz Krasiński, Joanna Sarnik, Janusz Blasiak, Jacek Waldmajer, Tomasz Poplawski
Great advances in biotechnology have allowed the construction of a computer from DNA. One of the proposed solutions is a biomolecular finite automaton, a simple two-state DNA computer without memory, which was presented by Ehud Shapiro's group at the Weizmann Institute of Science. The main problem with this computer, in which biomolecules carry out logical operations, is its complexity - increasing the number of states of biomolecular automata. In this study, we constructed (in laboratory conditions) a six-state DNA computer that uses two endonucleases (e...
April 22, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28432748/future-challenges-and-therapeutic-opportunities-in-type-2-diabetes-changing-the-paradigm-of-current-therapy
#17
REVIEW
D R Owens, L Monnier, A H Barnett
Most algorithms for type 2 diabetes mellitus (T2DM) do not recommend treatment escalation until glycated haemoglobin (HbA1c) fails to reach the recommended target of 7% (53 mmol/mol) within approximately 3 months on any treatment regimen ('treat to failure'). Clinical inertia and/or poor adherence to therapy contribute to patients not reaching glycaemic targets when managed according to this paradigm. Clinical inertia exists across the entire spectrum of anti-diabetes therapies, although it is most pronounced when initiating and optimizing insulin therapy...
April 21, 2017: Diabetes, Obesity & Metabolism
https://www.readbyqxmd.com/read/28432657/in-vitro-assay-for-measuring-real-time-topotecan-release-from-liposomes-release-kinetics-and-cellular-internalization
#18
Roger Gilabert-Oriol, Lina Chernov, Malathi Anantha, Wieslawa H Dragowska, Marcel B Bally
Topotecan is a drug that is under investigation for the treatment of neuroblastoma and has been encapsulated into liposomes to improve its therapeutic efficacy. However, liposomal formulations still need to be optimized for drug retention and new techniques to measure drug release are required to better understand this process. Here, a novel in vitro method based on fluorescence de-quenching and an automated microscopy imaging platform were developed for monitoring, in real time, the release of topotecan from a liposomal formulation...
April 21, 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/28432615/fabrication-physicochemical-characterization-and-performance-evaluation-of-biodegradable-polymeric-microneedle-patch-system-for-enhanced-transcutaneous-flux-of-high-molecular-weight-therapeutics
#19
Viral Shah, Bijaya Krushna Choudhury
A revolutionary paradigm shift is being observed currently, towards the use of therapeutic biologics for disease management. The present research was focused on designing an efficient dosage form for transdermal delivery of α-choriogonadotropin (high molecular weight biologic), through biodegradable polymeric microneedles. Polyvinylpyrrolidone-based biodegradable microneedle arrays loaded with high molecular weight polypeptide, α-choriogonadotropin, were fabricated for its systemic delivery via transdermal route...
April 21, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28432486/cholesterol-as-a-modifying-agent-of-the-neurovascular-unit-structure-and-function-under-physiological-and-pathological-conditions
#20
REVIEW
Ewelina Czuba, Aleksandra Steliga, Grażyna Lietzau, Przemysław Kowiański
The brain, demanding constant level of cholesterol, precisely controls its synthesis and homeostasis. The brain cholesterol pool is almost completely separated from the rest of the body by the functional blood-brain barrier (BBB). Only a part of cholesterol pool can be exchanged with the blood circulation in the form of the oxysterol metabolites such, as 27-hydroxycholesterol (27-OHC) and 24S-hydroxycholesterol (24S-OHC). Not only neurons but also blood vessels and neuroglia, constituting neurovascular unit (NVU), are crucial for the brain cholesterol metabolism and undergo precise regulation by numerous modulators, metabolites and signal molecules...
April 21, 2017: Metabolic Brain Disease
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