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https://www.readbyqxmd.com/read/28448464/bayesian-device-free-localization-and-tracking-in-a-binary-rf-sensor-network
#1
Zhenghuan Wang, Heng Liu, Shengxin Xu, Xiangyuan Bu, Jianping An
Received-signal-strength-based (RSS-based) device-free localization (DFL) is a promising technique since it is able to localize the person without attaching any electronic device. This technology requires measuring the RSS of all links in the network constituted by several radio frequency (RF) sensors. It is an energy-intensive task, especially when the RF sensors work in traditional work mode, in which the sensors directly send raw RSS measurements of all links to a base station (BS). The traditional work mode is unfavorable for the power constrained RF sensors because the amount of data delivery increases dramatically as the number of sensors grows...
April 27, 2017: Sensors
https://www.readbyqxmd.com/read/28446216/the-effect-of-adipose-tissue-derived-stem-cells-in-a-middle-cerebral-artery-occlusion-stroke-model-depends-on-their-engraftment-rate
#2
Saskia Grudzenski, Sebastian Baier, Anne Ebert, Pim Pullens, Andreas Lemke, Karen Bieback, Rick M Dijkhuizen, Lothar R Schad, Angelika Alonso, Michael G Hennerici, Marc Fatar
BACKGROUND: In the field of experimental stem cell therapy, intra-arterial (IA) delivery yields the best results concerning, for example, migrated cell number at the targeted site. However, IA application also appears to be associated with increased mortality rates and infarction. Since many rodent studies systemically apply 1 × 10(6) cells, this could also be a consequence of engrafted cell number. The aim of this study was therefore to investigate the effect of different doses of adipose tissue-derived stem cells (ASCs) on engraftment rates and stroke outcome measured in vivo using 9...
April 26, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28441601/improving-understanding-of-mixed-land-use-watershed-suspended-sediment-regimes-mechanistic-progress-through-high-frequency-sampling
#3
Elliott Kellner, Jason A Hubbart
Given the importance of suspended sediment to biogeochemical functioning of aquatic ecosystems, and the increasing concern of mixed-land-use effects on pollutant loading, there is an urgent need for research that quantitatively characterizes spatiotemporal variation of suspended sediment dynamics in contemporary watersheds. A study was conducted in a representative watershed of the central United States utilizing a nested-scale experimental watershed design, including five gauging sites (n=5) partitioning the catchment into five sub-watersheds...
April 22, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28438022/improvements-in-nanoparticle-tracking-analysis-to-measure-particle-aggregation-and-mass-distribution-a-case-study-on-engineered-nanomaterial-stability-in-incineration-landfill-leachates
#4
Kamyar Mehrabi, Bernd Nowack, Yadira Arroyo Rojas Dasilva, Denise M Mitrano
Numerous nanometrology techniques have been developed in recent years to determine the size, concentration and a number of other characteristics of engineered nanomaterials (ENM) in environmental matrices. Among the many available techniques, Nanoparticle Tracking Analysis (NTA) can measure individual particles to create a size distribution and measure the particle number. Therefore, we explore the possibility to use these data to calculate the particle mass distribution. Additionally, we further developed the NTA methodology to explore its suitability for analysis of ENM in complex matrices by measuring ENM agglomeration and sedimentation in municipal solid waste incineration landfill leachates over time...
April 24, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28436961/the-evolving-landscape-of-drug-products-containing-nanomaterials-in-the-united-states
#5
Sheetal R D'Mello, Celia N Cruz, Mei-Ling Chen, Mamta Kapoor, Sau L Lee, Katherine M Tyner
The Center for Drug Evaluation and Research (CDER) within the US Food and Drug Administration (FDA) is tracking the use of nanotechnology in drug products by building and interrogating a technical profile of products containing nanomaterials submitted to CDER. In this Analysis, data from more than 350 products show an increase in the submissions of drug products containing nanomaterials over the last two decades. Of these, 65% are investigational new drugs, 17% are new drug applications and 18% are abbreviated new drug applications, with the largest class of products being liposomal formulations intended for cancer treatments...
April 24, 2017: Nature Nanotechnology
https://www.readbyqxmd.com/read/28430109/acquisition-of-electrocardiogram-signals-during-magnetic-resonance-imaging
#6
Julien Oster, Gari Clifford
The recording of the electrocardiogram (ECG) during Magnetic Resonance Imaging (MRI) acquisition is of great interest and importance. Firstly, MRI acquisition is a relatively slow process, which therefore complicates the imaging of moving organs. Cardiac MRI requires the development of strategies for acquiring high quality images, which is mainly achieved by synchronising the image acquisition with a specific time during the cardiac cycle. The ECG is used to monitor the heart's activity, and the detection of the largest and steepest peak in the cardiac cycle (the QRS complex) triggers the acquisition of slices of the k-space...
April 21, 2017: Physiological Measurement
https://www.readbyqxmd.com/read/28428999/shear-induced-reversibility-of-2d-colloidal-suspensions-in-the-presence-of-minimal-thermal-noise
#7
Somayeh Farhadi, Paulo E Arratia
The effects of minimal thermal noise on particle rearrangements in cyclically sheared colloidal suspensions are experimentally investigated using particle tracking methods. Our experimental model system consists of polystyrene microspheres adsorbed at an oil-water interface, in which the particles exhibit small but non-negligible Brownian motion. Experiments are performed on bidisperse (1.0 and 1.2 μm in diameter) systems, which form area fractions of 0.20 and 0.32 at the interface. We first characterize the thermal (Brownian) noise using particle diffusivities at quiescent states, and show that under our experimental flow conditions both systems (0...
April 21, 2017: Soft Matter
https://www.readbyqxmd.com/read/28427436/a-3d-in-vitro-model-of-the-human-breast-duct-a-method-to-unravel-myoepithelial-luminal-interactions-in-the-progression-of-breast-cancer
#8
Edward P Carter, James A Gopsill, Jennifer J Gomm, J Louise Jones, Richard P Grose
BACKGROUND: 3D modelling fulfils a critical role in research, allowing for complex cell behaviour and interactions to be studied in physiomimetic conditions. With tissue banks becoming established for a number of cancers, researchers now have access to primary patient cells, providing the perfect building blocks to recreate and interrogate intricate cellular systems in the laboratory. The ducts of the human breast are composed of an inner layer of luminal cells supported by an outer layer of myoepithelial cells...
April 21, 2017: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/28422660/weak-classifier-for-density-estimation-in-eye-localization-and-tracking
#9
Gabriel M Araujo, Felipe M L Ribeiro, Waldir S Junior, Eduardo A B da Silva, Siome K Goldenstein
In this work, we propose a fast weak classifier that can detect and track eyes in video sequences. The approach relies on a least-squares detector based on the Inner Product Detector (IPD) that can estimate a probability density distribution for a feature's location - which fits naturally with a Bayesian estimation cycle, such as a Kalman or particle filter. As a leastsquares sliding window detector, it possesses tolerance to small variations in the desired pattern while maintaining good generalization capabilities and computational efficiency...
April 13, 2017: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/28419484/variations-in-the-functional-visual-field-for-detection-of-lung-nodules-on-chest-computed-tomography-impact-of-nodule-size-distance-and-local-lung-complexity
#10
Lukas Ebner, Martin Tall, Kingshuk Roy Choudhury, Donald L Ly, Justus E Roos, Sandy Napel, Geoffrey D Rubin
PURPOSE: To explore the characteristics that impact lung nodule detection by peripheral vision when searching for lung nodules on chest CT-scans. METHODS: This study was approved by the local IRB and HIPAA compliant. A simulated primary (1°) target mass (2 x 2 x 5 cm) was embedded into 5-cm thick sub-volumes (SV) extracted from three unenhanced lung MDCT-scans (64-row, 1.25 mm thickness, 0.7 mm increment). One of 30 solid, secondary nodules with either 3-4 mm and 5-8 mm diameters were embedded into 192 of 207 SVs...
April 17, 2017: Medical Physics
https://www.readbyqxmd.com/read/28415542/bladder-smooth-muscle-cells-on-electrospun-poly-%C3%AE%C2%B5-caprolactone-poly-l-lactic-acid-scaffold-promote-bladder-regeneration-in-a-canine-model
#11
Nasser Shakhssalim, Masoud Soleimani, Mohammad M Dehghan, Javad Rasouli, Masoud Taghizadeh-Jahed, Peyman M Torbati, Mohammad Naji
Engineering of urinary bladder has been the focus of numerous studies in recent decade. Novel biomaterials, innovative fabrication methods and various modification processes of scaffolds are the critical issues to find supportive matrices. Supportive characteristics of electrospun PCL/PLLA nano-scaffold for bladder augmentation in canine model and the role of bladder cells in regeneration process were appraised. Electrospun PCL/PLLA was fabricated by co-electrospinning of PCL and PLLA. Bladder cells were isolated and transduced with lentiviral particles encoding eGFP and JRed proteins...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28415349/decorrelation-correction-for-nanoparticle-tracking-analysis-of-dilute-polydisperse-suspensions-in-bulk-flow
#12
John Hartman, Brian Kirby
Nanoparticle tracking analysis, a multiprobe single particle tracking technique, is a widely used method to quickly determine the concentration and size distribution of colloidal particle suspensions. Many popular tools remove non-Brownian components of particle motion by subtracting the ensemble-average displacement at each time step, which is termed dedrifting. Though critical for accurate size measurements, dedrifting is shown here to introduce significant biasing error and can fundamentally limit the dynamic range of particle size that can be measured for dilute heterogeneous suspensions such as biological extracellular vesicles...
March 2017: Physical Review. E
https://www.readbyqxmd.com/read/28415337/mean-squared-displacement-statistical-test-for-fractional-brownian-motion
#13
Grzegorz Sikora, Krzysztof Burnecki, Agnieszka Wyłomańska
Anomalous diffusion in crowded fluids, e.g., in cytoplasm of living cells, is a frequent phenomenon. A common tool by which the anomalous diffusion of a single particle can be classified is the time-averaged mean square displacement (TAMSD). A classical mechanism leading to the anomalous diffusion is the fractional Brownian motion (FBM). A validation of such process for single-particle tracking data is of great interest for experimentalists. In this paper we propose a rigorous statistical test for FBM based on TAMSD...
March 2017: Physical Review. E
https://www.readbyqxmd.com/read/28415277/sintering-of-polydisperse-viscous-droplets
#14
Fabian B Wadsworth, Jérémie Vasseur, Edward W Llewellin, Donald B Dingwell
Sintering-or coalescence-of compacts of viscous droplets is driven by the interfacial tension between the droplets and the interstitial gas phase. The process, which occurs in a range of industrial and natural settings, such as the manufacture of ceramics and the welding of volcanic ash, causes the compact to densify, to become stronger, and to become less permeable. We investigate the role of droplet polydispersivity in sintering dynamics by conducting experiments in which populations of glass spheres with different size distributions are heated to temperatures above the glass transition interval...
March 2017: Physical Review. E
https://www.readbyqxmd.com/read/28415019/recovery-of-infective-virus-particles-in-ion-exchange-and-hydrophobic-interaction-monolith-chromatography-is-influenced-by-particle-charge-and-total-to-infective-particle-ratio
#15
Dora Sviben, Dubravko Forcic, Jelena Ivancic-Jelecki, Beata Halassy, Marija Brgles
Viral particles are used in medical applications as vaccines or gene therapy vectors. In order to obtain product of high purity, potency and safety for medical use purification of virus particles is a prerequisite, and chromatography is gaining increased attention to meet this aim. Here, we report on the use of ion-exchange and hydrophobic interaction chromatography on monolithic columns for purification of mumps virus (MuV) and measles virus (MeV). Efficiency of the process was monitored by quantification of infective virus particles (by 50% cell culture infective dose assay) and total virus particles, and monitoring of their size (by Nanoparticle Tracking Analysis)...
April 8, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28410875/particle-sorting-by-paramecium-cilia-arrays
#16
Richard Mayne, James G H Whiting, Gabrielle Wheway, Chris Melhuish, Andrew Adamatzky
Motile cilia are cell-surface organelles whose purposes, in ciliated protists and certain ciliated metazoan epithelia, include generating fluid flow, sensing and substance uptake. Certain properties of cilia arrays, such as beating synchronisation and manipulation of external proximate particulate matter, are considered emergent, but remain incompletely characterised despite these phenomena having being the subject of extensive modelling. This study constitutes a laboratory experimental characterisation of one of the emergent properties of motile cilia: manipulation of adjacent particulates...
April 12, 2017: Bio Systems
https://www.readbyqxmd.com/read/28403228/refining-particle-positions-using-circular-symmetry
#17
Alvaro Rodriguez, Hanqing Zhang, Krister Wiklund, Tomas Brodin, Jonatan Klaminder, Patrik Andersson, Magnus Andersson
Particle and object tracking is gaining attention in industrial applications and is commonly applied in: colloidal, biophysical, ecological, and micro-fluidic research. Reliable tracking information is heavily dependent on the system under study and algorithms that correctly determine particle position between images. However, in a real environmental context with the presence of noise including particular or dissolved matter in water, and low and fluctuating light conditions, many algorithms fail to obtain reliable information...
2017: PloS One
https://www.readbyqxmd.com/read/28402886/three-dimensional-super-resolution-in-eukaryotic-cells-using-the-double-helix-point-spread-function
#18
Alexander R Carr, Aleks Ponjavic, Srinjan Basu, James McColl, Ana Mafalda Santos, Simon Davis, Ernest D Laue, David Klenerman, Steven F Lee
Single-molecule localization microscopy, typically based on total internal reflection illumination, has taken our understanding of protein organization and dynamics in cells beyond the diffraction limit. However, biological systems exist in a complicated three-dimensional environment, which has required the development of new techniques, including the double-helix point spread function (DHPSF), to accurately visualize biological processes. The application of the DHPSF approach has so far been limited to the study of relatively small prokaryotic cells...
April 11, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28402875/tracking-low-copy-transcription-factors-in-living-bacteria-the-case-of-the-lac-repressor
#19
Federico Garza de Leon, Laura Sellars, Mathew Stracy, Stephen J W Busby, Achillefs N Kapanidis
Transcription factors control the expression of genes by binding to specific sites in DNA and repressing or activating transcription in response to stimuli. The lac repressor (LacI) is a well characterized transcription factor that regulates the ability of bacterial cells to uptake and metabolize lactose. Here, we study the intracellular mobility and spatial distribution of LacI in live bacteria using photoactivated localization microscopy combined with single-particle tracking. Since we track single LacI molecules in live cells by stochastically photoactivating and observing fluorescent proteins individually, there are no limitations on the copy number of the protein under study; as a result, we were able to study the behavior of LacI in bacterial strains containing the natural copy numbers (∼40 monomers), as well as in strains with much higher copy numbers due to LacI overexpression...
April 11, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28398558/studying-the-structural-optical-chemical-and-electrochemical-etching-changes-of-cr-39-for-dosemetric-applications
#20
M F Zaki, Y H Elshaer, Doaa H Taha
The present work shows the induced modification of the structural, optical, chemical etching and electrochemical etching parameters of CR-39 irradiated with alpha-particles. CR-39 polymer track detectors were irradiated with different fluences (1.62 × 106, 2.72 × 106, 3.82 × 106 and 5.21 × 106 particles/cm2) of alpha-particles using 241Am source. The structural and optical properties were measured by FT-IR spectroscopy, X-ray diffraction and UV/Vis spectroscopy, respectively. The FT-IR spectra reveal that no major changes in the typical functional groups of irradiated polymer detectors...
April 7, 2017: Radiation Protection Dosimetry
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