Read by QxMD icon Read


Bizhan Malaekeh-Nikouei, Leila Gholami, Fariba Asghari, Saeedeh Askarian, Saeedeh Barzegar, Mehdi Rezaee, Reza Kazemi Oskuee
Non-viral vectors such as polymers and liposomes have been used as gene delivery systems to overcome intrinsic problems of viral vectors, but transfection efficiency of these vectors is lower than viral vectors. In the present study, we tried to design non-viral gene delivery vectors that mimic the viral vectors using the benefits of both cationic liposomes and cationic polymer vectors along with targeting glucocorticoid receptors to enhance cellular trafficking of vectors. Cationic liposomes containing DOTAP and cholesterol were prepared by thin-film hydration following extrusion method...
February 21, 2018: Colloids and Surfaces. B, Biointerfaces
E Ghazizadeh, R K Oskuee, M R Jaafari, S Hosseinkhani
The present study aim to design a liposomal electrochemical sensor using 1, 2-dioleoyl-3-trimethylammoniumpropane (DOTAP) and dioleoylphosphatidylethanolamine(DOPE), chimeric probes and p19, it has been considered as a caliper molecule as well. Also the competitor structural hybrid (RNA) was used to detect three types of miRs in one screen printed electrode modified by gold nanoparticle (SCPE/GNP). In this purpose, the sensor signal stabilized when the cationic DOTAP-DOPE with hybrids of the chimeric probes (Stem, M-linear) sandwiched in order to detect 221-124a miRs...
February 28, 2018: Scientific Reports
Nhiem Tran, Marion Hocquet, Blandine Eon, Parveen Sangwan, Julian Ratcliffe, Tracey M Hinton, Jacinta White, Berkay Ozcelik, Nicholas P Reynolds, Benjamin W Muir
The fight against infection in an era of emerging antibiotic resistant bacteria is one of the grandest scientific challenges facing society today. Nano-carriers show great promise in improving the antibacterial activity of antibiotics as they are able to enhance their solubility, provide sustained release and reduce toxic side effects via specifically targeting infection sites. Here, we investigate the antibacterial effect of two lipidic nano-carriers that contain the poorly soluble antibiotic rifampicin in their bilayers...
February 16, 2018: Journal of Colloid and Interface Science
Feng Wan, Tommy Nylander, Sylvia Natalie Klodzinska, Camilla Foged, Mingshi Yang, Stefania G Baldursdottir, Hanne Mørck Nielsen
Nanoparticle (NP) mediated drug delivery into viscous biomatrices, e.g., mucus and bacterial biofilms, is challenging. Lipid shell-enveloped polymeric NPs (Lipid@NPs), composed of a polymeric NP core coated with a lipid shell, represent a promising alternative to the current delivery systems. Here, we describe facile methods to prepare Lipid@NPs with high integrity of lipid shells and demonstrate the potential of Lipid@NPs in effective mucus penetration and interaction with cystic fibrosis-related bacterial biofilms...
February 23, 2018: ACS Applied Materials & Interfaces
Thomas Fuhs, Lasse Hyldgaard Klausen, Steffan Møller Sønderskov, Xiaojun Han, Mingdong Dong
The local surface charge density of the cell membrane influences regulation and localization of membrane proteins. The local surface charge density could, until recently, not be measured directly under physiological conditions, and it was largely a hypothetical yet very important parameter. Here we use unsaturated lipids of a distinct charge (DOTAP, DOPC, and DOPG) and a neutral fully saturated lipid (DPPC) to create model membranes with phase separating domains of a defined charge. We then apply quantitative surface charge microscopy (QSCM) to investigate the local surface charge density; this is a technique based on a scanning ion conductance microscope (SICM) capable of measuring surface charge density with nanoscale lateral resolution...
February 20, 2018: Nanoscale
Wenjing Qu, Na Li, Rui Yu, Wenbao Zuo, Tingting Fu, Wenling Fei, Yanhui Hou, Yanhua Liu, Jianhong Yang
The cationic dimethyldioctadecylammonium/trehalose 6,6,9-dibehenate (DDA/TDB) liposome is as a strong adjuvant system for vaccines, with remarkable immunostimulatory activity. The mucosal administration of vaccines is a potential strategy for inducing earlier and stronger mucosal immune responses to infectious diseases. In this study, we assessed whether the intranasal administration of cationic DDA/TDB liposomes combined with influenza antigen A (H3N2) can be used as a highly efficacious vaccine to induce mucosal and systemic antibody responses...
February 15, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Elisa Zagato, Lotte Vermeulen, Heleen Dewitte, Griet Van Imschoot, Roosmarijn E Vandenbroucke, Jo Demeester, Stefaan C De Schmedt, Kristiaan Neyts, Katrien Remaut, Kevin Braeckmans
Nucleic acid biopharmaceuticals are being investigated as potential therapeutics. They need to be incorporated into a bio-compatible carrier so as to overcome several biological barriers. Rational development of suitable nanocarriers requires high quality characterization techniques. While size, concentration and stability can be very well measured these days, even in complex biological fluids, a method to accurately quantify the number of nucleic acid therapeutics encapsulated in nanocarriers is still missing...
February 12, 2018: Molecular Pharmaceutics
Hong Li, Yang Liu, Lihua Chen, Qibing Liu, Shanshan Qi, Xinwei Cheng, Young B Lee, Chang-Ho Ahn, Deog Joong Kim, Robert J Lee
Folate receptor-targeted lipid-albumin nanoparticles (F-LAN) were synthesized for delivery of RX-0201, an antisense oligonucleotide (ASO) against the Akt1, which is currently in a Phase IIa clinical trial for metastatic renal cancer. These nanoparticles were composed of DOTAP/soyPC/TPGS/folate-PEG-DSPE (25:70:4:1 m/m), a cationic human serum albumin-pentaethylenehexamine (HSA-PEHA) conjugate, and RX-0201. The F-LAN-RX-0201 had a mean particle size of 108.6±5.8 nm, zeta potential of 10.5±3.2 mV, and ASO loading efficiency of 71...
January 29, 2018: Journal of Drug Targeting
Guoqing Xu, Changchun Hao, Lei Zhang, Runguang Sun
In this article, the interaction between bovine serum albumin (BSA) and the cationic 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) at the air-buffer interface was investigated at different subphase's pH values (pH = 3, 5 and 10). Surface pressure measurements (π - A) and penetration kinetics process (π - t) were carried out to reveal the interaction mechanism and the dynamical behavior. The data showed that π - A isotherms moved towards larger mean molecular area when the concentration of BSA ([BSA]) increased, the amount of BSA adsorbed onto DOTAP monolayer reached a threshold value at a [BSA] of 5 × 10-8 M, and BSA desorbed from the lipid monolayer as time goes by...
January 10, 2018: Scientific Reports
Iman Jafari, Vahid Heravi Shargh, Maryam Shahryari, Azam Abbasi, Mahmoud Reza Jaafari, Ali Khamesipour, Ali Badiee
Although there have been numerous attempts to develop a successful vaccine against leishmaniasis, based on the clinical trial in this field, no vaccine against Leishmania in routine way can be found for globally effective vaccination in human. Amongst, first generation vaccines consisting of parasite fractions or whole killed Leishmania showed more successful results in clinical trials. It seems that the main reason for the low efficacy of these vaccines is lack of a suitable adjuvant. In this study, a crude extract of detergent-solubilized L...
December 28, 2017: Immunobiology
Yoonhee Bae, Min Kyo Jung, Seulgi Lee, Su Jeong Song, Ji Young Mun, Eric S Green, Jin Han, Kyung Soo Ko, Joon Sig Choi
Mitochondria are targets with great potential for therapeutics for many human disorders. However, drug delivery systems for such therapeutics remain in need of more efficient mitochondrial-targeting carriers. In this study, we report that nanosomes composed of Dequalinium/DOTAP (1,2-dioleoyl-3-trimethylammonium-propane)/DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine), called DQA80s, can act in the dual role of mitochondrial-targeting carrier and anticancer agent for therapeutic interventions against mitochondrial diseases...
January 2, 2018: European Journal of Pharmaceutics and Biopharmaceutics
Shun-Fu Chang, Chih-Chang Yeh, Pin-Jyun Chen, Hsin-I Chang
Recent studies have demonstrated that fat accumulation in bone cells is detrimental to bone mass. Both adipocytes and osteoblasts are derived from common multipotent mesenchymal stem cells (MSCs) and hence the presence of fat may increase adipocyte proliferation, differentiation and fat accumulation while inhibiting osteoblast differentiation and bone formation. Lipids are common constituents in supramolecular vesicles (e.g., micelles or liposomes) that serve as drug delivery systems. Liposomal formulations such as Meriva® were proven to decrease joint pain and improve joint function in osteoarthritis (OA) patients...
January 2, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Peng Wang, Lingmin Zhang, Wenfu Zheng, Liman Cong, Zhaorong Guo, Yangzhouyun Xie, Le Wang, Rongbing Tang, Qiang Feng, Yoh Hamada, Kohsuke Gonda, Zhijian Hu, Xiaochun Wu, Xingyu Jiang
CRISPR/Cas9 system is a powerful toolbox for gene editing. However, the low delivery efficiency is still a big hurdle influencing its applications. Herein, we report a strategy to deliver Cas9-sgPlk-1 plasmids (CP) by a multifunctional vehicle for tumor therapy. We condensed CP on TAT peptide-modified Au nanoparticles (AuNPs/CP, ACP) via electrostatic interactions, and coated lipids (DOTAP, DOPE, Cholesterol, PEG2000-DSPE) on the ACP to form lipid-encapsulated, AuNPs-condensed CP (LACP). LACP can enter tumor cells and release the CP into the cytosol by laser-triggered thermo-effects of the AuNPs and the CP can enter nuclei by TAT guidance, enabling effective knock-outs of target gene (Plk-1) of tumor (melanoma) and inhibition of the tumor both in vitro and in vivo...
December 28, 2017: Angewandte Chemie
Antje Ziller, Sara S Nogueira, Eva Huehn, Sergio S Funari, Gerald Brezesinski, Hermann Hartmann, Ugur Sahin, Heinrich Haas, Peter Langguth
Insertion of high molecular weight messenger RNA (mRNA) into lyotropic lipid phases as model systems for controlled release formulations for the mRNA was investigated. Low fractions of 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) were used as an anchor to load the mRNA into a lamellar lipid matrix. Dispersions of zwitterionic lipid in the aqueous phase in the presence of increasing fractions of mRNA and cationic lipid were prepared, and the molecular organization was investigated as a function of mRNA and cationic lipid fraction...
December 12, 2017: Molecular Pharmaceutics
Juanjuan Yang, Changchun Hao, Runguang Sun
In this paper, we examined the effect of Laminaria japonica polysaccharides (LJP) on cationic 1,2-Dioleoyl-3-Trimethylammonium-Propane (DOTAP) and anionic 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-1-glycerol] (DPPG) monolayers at the air-water interface by the pressure-area isotherms (π-A), adsorption curves (π-t) and morphology measurements with atomic force microscopy (AFM) technique. The π-A curves revealed that the isotherms shifted to larger mean molecular area with progressive addition of LJP into subphase for both DOTAP and DPPG monolayers...
January 1, 2018: Colloids and Surfaces. B, Biointerfaces
Baoliang Ma, Xiaofei Wang, Yujie Liu, Zhihong Li, Yujie Liu, Fan Zhang
Protein aggregation is a prevalent phenomenon. It is important to study protein aggregation under different solution conditions. In this study, using 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence spectra, we investigated the critical micelle concentration (CMC) of 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). We also studied the effects of DOTAP on amyloid aggregation of β-lactoglobulin using intrinsic fluorescence spectra, circular dichroism spectra, thioflavin T fluorescence, a Congo red binding assay and transmission electron microscopy...
November 8, 2017: Chemistry and Physics of Lipids
Roselena Silvestri Schuh, Talita Giacomet de Carvalho, Roberto Giugliani, Ursula Matte, Guilherme Baldo, Helder Ferreira Teixeira
Mucopolysaccharidosis type I (MPS I) is an inherited disease caused by the deficiency of alpha-L-iduronidase (IDUA). This study shows the use of nanoemulsions co-complexed with the plasmid of CRISPR/Cas9 system and a donor oligonucleotide aiming at MPS I gene editing in vitro. Nanoemulsions composed of MCT, DOPE, DOTAP, DSPE-PEG, and water were prepared by high-pressure homogenization. The DNA was complexed by adsorption or encapsulation of preformed DNA/DOTAP complexes with nanoemulsions at +4/ -1 charge ratio...
November 6, 2017: European Journal of Pharmaceutics and Biopharmaceutics
Soo-Yeon Kim, Sang-Jin Lee, Jin-Ki Kim, Han-Gon Choi, Soo-Jeong Lim
Cationic lipid-based nanoparticles enhance viral gene transfer by forming electrostatic complexes with adenoviral vectors. We recently demonstrated the superior complexation capabilities of 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) emulsion compared with a liposomal counterpart but the cytotoxicity of DOTAP emulsions remained a challenge. The present study is aimed at formulating an emulsion capable of acting as a highly effective viral gene transfer vehicle with reduced cytotoxicity and to physicochemically characterize the structures of virus-emulsion complexes in comparison with virus-liposome complexes when the only difference between emulsions and liposomes was the presence or absence of inner oil core...
2017: International Journal of Nanomedicine
Paola Astolfi, Elisabetta Giorgini, Valentina Gambini, Barbara Rossi, Lisa Vaccari, Francesco Vita, Oriano Francescangeli, Cristina Marchini, Michela Pisani
Lyotropic cubic liquid-crystalline systems have received increasing attention due to their unique microstructural and physicochemical properties as efficient nanocarriers for drug delivery. We report the preparation and characterization of bulk phases and cubosome dispersions of phytantriol loaded with the anticancer drug 5-fluorouracil, in neutral and anionic forms. In both cases, a Pn3m cubic phase was observed. The phytantriol phase behavior can be influenced by the addition of ionic agents, and, to this purpose, a positively charged lipid, such as N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride salt (DOTAP), was included in the studied formulations...
October 23, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
C Facundo Temprana, M Jimena Prieto, Daniela E Igartúa, A Lis Femia, M Silvia Amor, Silvia Del Valle Alonso
Different viral and non-viral vectors have been designed to allow the delivery of nucleic acids in gene therapy. In general, non-viral vectors have been associated with increased safety for in vivo use; however, issues regarding their efficacy, toxicity and stability continue to drive further research. Thus, the aim of this study was to evaluate the potential use of the polymerizable diacetylenic lipid 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DC8,9PC) as a strategy to formulate stable cationic lipopolymers in the delivery and protection of plasmid DNA...
2017: PloS One
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"