keyword
https://read.qxmd.com/read/36202617/modulation-of-hippocampal-network-oscillation-by-pick1-dependent-cell-surface-expression-of-mglu3-receptors
#21
JOURNAL ARTICLE
Pola Tuduri, Nathalie Bouquier, Benoit Girard, Enora Moutin, Maxime Thouaye, Julie Perroy, Federica Bertaso, Jeanne Ster
Metabotropic glutamate receptor type 3 (mGlu3) controls the sleep/wake architecture which plays a role in the glutamatergic pathophysiology of schizophrenia. Interestingly, mGlu3 receptors expression is decreased in the brain of schizophrenic patients. However, little is known about the molecular mechanisms regulating mGlu3 receptors at the cell membrane. Subcellular receptor localization is strongly dependent on protein-protein interactions. Here we show that mGlu3 interacts with PICK1 and that this scaffolding protein is important for mGlu3 surface expression and function in hippocampal primary cultures...
October 6, 2022: Journal of Neuroscience
https://read.qxmd.com/read/36047070/globozoospermia-a-case-report-and-systematic-review-of-literature
#22
JOURNAL ARTICLE
Andrea Crafa, Rosita Angela Condorelli, Sandro La Vignera, Aldo Eugenio Calogero, Rossella Cannarella
PURPOSE: Globozoospermia is a genetic syndrome characterized by the presence of round-headed spermatozoa and infertility due to the inability of these spermatozoa to fertilize the oocyte. In this article, we present the clinical case of a young globozoospermic patient with a new, not yet described mutation of the DPY19L2 gene. We also performed a systematic review of the literature on gene mutations, the outcome of assisted reproductive techniques, and the risk of transmission of abnormalities to the offspring in patients with globozoospermia and made recommendations to offer a more appropriate clinical management of these patients...
July 6, 2022: World Journal of Men's Health
https://read.qxmd.com/read/35954295/three-binding-conformations-of-bio124-in-the-pocket-of-the-pick1-pdz-domain
#23
JOURNAL ARTICLE
Amy O Stevens, Samuel Luo, Yi He
The PDZ family has drawn attention as possible drug targets because of the domains' wide ranges of function and highly conserved binding pockets. The PICK1 PDZ domain has been proposed as a possible drug target because the interactions between the PICK1 PDZ domain and the GluA2 subunit of the AMPA receptor have been shown to progress neurodegenerative diseases. BIO124 has been identified as a sub µM inhibitor of the PICK1-GluA2 interaction. Here, we use all-atom molecular dynamics simulations to reveal the atomic-level interaction pattern between the PICK1 PDZ domain and BIO124...
August 7, 2022: Cells
https://read.qxmd.com/read/35774118/a-role-of-the-tex101-interactome-in-the-common-aetiology-behind-male-subfertility-and-testicular-germ-cell-tumor
#24
REVIEW
Joshua Burton, Marcin W Wojewodzic, Trine B Rounge, Trine B Haugen
Patients who develop testicular germ cell tumours (TGCT) are at higher risk to be subfertile than the general population. The conditions are believed to originate during foetal life, however, the mechanisms behind a common aetiology of TGCT and male subfertility remains unknown. Testis-expressed 101 (TEX101) is a glycoprotein that is related to male fertility, and downregulation of the TEX101 gene was shown in pre-diagnostic TGCT patients. In this review, we summarize the current knowledge of TEX101 and its interactome related to fertility and TGCT development...
2022: Frontiers in Oncology
https://read.qxmd.com/read/35721319/ca-2-regulates-dimerization-of-the-bar-domain-protein-pick1-and-consequent-membrane-curvature
#25
JOURNAL ARTICLE
Georgiana F Stan, Deborah K Shoemark, Dominic Alibhai, Jonathan G Hanley
Bin-Amphiphysin-Rvs (BAR) domain proteins are critical regulators of membrane geometry. They induce and stabilize membrane curvature for processes, such as clathrin-coated pit formation and endosomal membrane tubulation. BAR domains form their characteristic crescent-shaped structure in the dimeric form, indicating that the formation of the dimer is critical to their function of inducing membrane curvature and suggesting that a dynamic monomer-dimer equilibrium regulated by cellular signaling would be a powerful mechanism for controlling BAR domain protein function...
2022: Frontiers in Molecular Neuroscience
https://read.qxmd.com/read/35505883/characterization-of-novel-lncrna-muscle-expression-profiles-associated-with-meat-quality-in-beef-cattle
#26
JOURNAL ARTICLE
Maria Malane Magalhães Muniz, Larissa Fernanda Simielli Fonseca, Daiane Cristina Becker Scalez, Aroa Suarez Vega, Danielly Beraldo Dos Santos Silva, Jesus Aparecido Ferro, Artur Loyola Chardulo, Fernando Baldi, Angela Cánovas, Lucia Galvão de Albuquerque
The aim of this study was to identify novel lncRNA differentially expressed (DE) between divergent animals for beef tenderness and marbling traits in Nellore cattle. Longissimus thoracis muscle samples from the 20 most extreme bulls (of 80 bulls set) for tenderness, tender ( n  = 10) and tough ( n  = 10) groups, and marbling trait, high ( n  = 10) and low ( n  = 10) groups were used to perform transcriptomic analysis using RNA-Sequencing. For tenderness, 29 lncRNA were DE ( p -value ≤ 0.01) in tough beef animals in relation to tender beef animals...
April 2022: Evolutionary Applications
https://read.qxmd.com/read/35455935/the-scaffold-protein-pick1-as-a-target-in-chronic-pain
#27
REVIEW
Andreas Toft Sørensen, Joscha Rombach, Ulrik Gether, Kenneth Lindegaard Madsen
Well-tolerated and effective drugs for treating chronic pain conditions are urgently needed. Most chronic pain patients are not effectively relieved from their pain and suffer from debilitating drug side effects. This has not only drastic negative consequences for the patients' quality of life, but also constitute an enormous burden on society. It is therefore of great interest to explore new potent targets for effective pain treatment with fewer side effects and without addiction liability. A critical component of chronic pain conditions is central sensitization, which involves the reorganization and strengthening of synaptic transmission within nociceptive pathways...
April 7, 2022: Cells
https://read.qxmd.com/read/35381522/repression-of-autophagy-leads-to-acrosome-biogenesis-disruption-caused-by-a-sub-chronic-oral-administration-of-polystyrene-nanoparticles
#28
JOURNAL ARTICLE
Lixiao Zhou, Ziying Yu, Yinyin Xia, Shuqun Cheng, Jieying Gao, Wei Sun, Xuejun Jiang, Jun Zhang, Lejiao Mao, Xia Qin, Zhen Zou, Jingfu Qiu, Chengzhi Chen
As a new widespread contaminant, nanoplastics (NPs) pose a potential risk to human health. Nevertheless, the adverse effects of NPs on the male reproductive system are poorly understood. In this study, we aimed to determine the effects of polystyrene nanoplastics (PS-NPs) (50 nm) on sperm quality, with a focus on the acrosome defects. After 35 days of intragastric administration, sperm quality was decreased and testicular structures were impaired in mice exposed to PS-NPs in both the medium (1.0 mg/kg) and high dose (10 mg/kg) groups...
May 2022: Environment International
https://read.qxmd.com/read/35077398/coding-variants-identified-in-patients-with-diabetes-alter-pick1-bar-domain-function-in-insulin-granule-biogenesis
#29
JOURNAL ARTICLE
Rita C Andersen, Jan H Schmidt, Joscha Rombach, Matthew D Lycas, Nikolaj R Christensen, Viktor K Lund, Donnie S Stapleton, Signe S Pedersen, Mathias A Olsen, Mikkel Stoklund, Gith Noes-Holt, Tommas Te Nielsen, Mark P Keller, Anna M Jansen, Rasmus Herlo, Massimo Pietropaolo, Jens B Simonsen, Ole Kjærulff, Birgitte Holst, Alan D Attie, Ulrik Gether, Kenneth L Madsen
Bin/amphiphysin/Rvs (BAR) domains are positively charged crescent-shaped modules that mediate curvature of negatively charged lipid membranes during remodeling processes. The BAR domain proteins PICK1, ICA69, and the arfaptins have recently been demonstrated to coordinate the budding and formation of immature secretory granules (ISGs) at the trans-Golgi network. Here, we identify 4 coding variants in the PICK1 gene from a whole-exome screening of Danish patients with diabetes that each involve a change in positively charged residues in the PICK1 BAR domain...
March 1, 2022: Journal of Clinical Investigation
https://read.qxmd.com/read/34809533/ampa-glua2-subunit-competitive-inhibitors-for-pick1-pdz-domain-pharmacophore-based-virtual-screening-molecular-docking-molecular-dynamics-simulation-and-adme-studies
#30
JOURNAL ARTICLE
Shravan B Rathod, Pravin B Prajapati, Ranjan Pal, Mohmedyasin F Mansuri
PICK1 (Protein interacting with C kinase-1) plays a key role in the regulation of intracellular trafficking of AMPA GluA2 subunit that is linked with synaptic plasticity. PICK1 is a scaffolding protein and binds numerous proteins through its PDZ domain. Research showed that synaptic plasticity is altered upon disrupting the GluA2-PDZ interactions. Inhibiting PDZ and GluA2 binding lead to beneficial effects in the cure of neurological diseases thus, targeting PDZ domain is proposed as a novel therapeutic target in such diseases...
November 23, 2021: Journal of Biomolecular Structure & Dynamics
https://read.qxmd.com/read/34673136/electroacupuncture-prevents-lps-induced-neuroinflammation-via-upregulation-of-pick-tlr4-complexes-in-the-microglia-of-hippocampus
#31
JOURNAL ARTICLE
Yunchang Mo, Lu Wang, Miao Ren, Wenjing Xie, Xiaoxiao Ye, Bingbing Zhou, Anqi Zhang, Qinxue Dai, Junlu Wang
Sepsis-associated encephalopathy (SAE) is a common complication of sepsis caused by neuroinflammation. Electroacupuncture (EA) can be used to treat SAE, but the underlying mechanism is not clear. Lack of PICK1 further aggravates the inflammatory response in mice with sepsis. Therefore, we sought to investigate whether PICK1 is involved in the protective effects of electroacupuncture to SAE. In this study, mice were treated with EA after lipopolysaccharide (LPS) treatment. Behavioral tests; microglial activity of hippocampus; neuron survival and the inflammatory factors PICK1 and TLR4, as well as TLR4-related proteins, such as ERK, JNK, and P38, were assessed after EA treatment...
December 2021: Brain Research Bulletin
https://read.qxmd.com/read/34582111/fsc231-alleviates-paclitaxel-induced-neuralgia-by-inhibiting-the-interactions-between-pick1-and-glua2-and-activates-gsk-3%C3%AE-and-erk1-2
#32
JOURNAL ARTICLE
Xi Zhang, Jiagao Wang, Ran Ran, Yuchuan Peng, Yun Xiao
BACKGROUND: FSC231, a PSD-95/DLG/ZO-1 (PDZ) domain inhibitor of protein kinase Cα interacting protein 1 (PICK1), has analgesic effects, but the mechanism remains unclear. METHODS: The expression level of PICK1 in dorsal root ganglion (DRG) of rats was changed by vector plasmid, and the effect of PICK1 on paclitaxel (PTL)-induced neuralgia of rats was observed in collaboration with FSC231 treatment. The possible molecular mechanisms were explored by quantitative real-time polymerase chain reaction (qRT-PCR), Western Blot and co-immunoprecipitation (Co-IP) techniques...
November 2021: Brain and Behavior
https://read.qxmd.com/read/34500285/the-pick1-tlr4-complex-on-microglia-is-involved-in-the-regulation-of-lps-induced-sepsis-associated-encephalopathy
#33
JOURNAL ARTICLE
Lu Wang, Feihong Lin, Miao Ren, Xia Liu, Wenjing Xie, Anqi Zhang, Meizi Qian, Yunchang Mo, Junlu Wang, Ya Lv
The treatment options for sepsis-associated encephalopathy caused by systemic inflammation are still not sufficient. Protein kinase C interaction protein 1 (PICK1) has attracted much attention because of its important physiological functions in many tissues. However, its role in sepsis-associated encephalopathy remains elusive. Our study results revealed that the expression levels of PICK1 protein in mice with lipopolysaccharide-induced sepsis-associated encephalopathy were not significantly changed, but PICK1 deficiency led to excessive activation of microglia and Toll-like receptor (TLR)4 pathways, which aggravated the sepsis- associated encephalopathy...
November 2021: International Immunopharmacology
https://read.qxmd.com/read/34307672/pick1-deficiency-exacerbates-sepsis-associated-acute-kidney-injury
#34
JOURNAL ARTICLE
Qian Dou, Hang Tong, Yichun Yang, Han Zhang, Hua Gan
We performed in vitro and in vivo experiments to explore the role of protein kinase C-binding protein 1 (PICK1), an intracellular transporter involved in oxidative stress-related neuronal diseases, in sepsis-related acute kidney injury (AKI). Firstly, PCR, western blotting, and immunohistochemistry were used to observe the expression of PICK1 after lipopolysaccharide- (LPS-) induced AKI. Secondly, by inhibiting PICK1 in vivo and silencing PICK1 in vitro, we further explored the effect of PICK1 on AKI. Finally, the relationship between PICK1 and oxidative stress and the related mechanisms were explored...
2021: BioMed Research International
https://read.qxmd.com/read/34209343/molecular-analysis-of-dpy19l2-pick1-and-spata16-in-italian-unrelated-globozoospermic-men
#35
JOURNAL ARTICLE
Fabiana Faja, Francesco Pallotti, Francesco Cargnelutti, Giulia Senofonte, Tania Carlini, Andrea Lenzi, Francesco Lombardo, Donatella Paoli
This study aims to evaluate genetic contribution and sperm DNA fragmentation (SDF) in a cohort of 18 unrelated globozoospermic Italian men (Group G). Semen samples were assessed according to the WHO 2010 Laboratory Manual and compared with 31 fertile controls. We focused our genetic analysis on the exons of the main globozoospermia-associated genes, performing qualitative PCR to assess deletion of DPY19L2 and sequencing to detect mutations of SPATA16 and PICK1 . SDF was evaluated using the TUNEL assay. In Group G, 10 patients had a complete form of globozoospermia, whereas 8 patients had a partial form...
June 30, 2021: Life
https://read.qxmd.com/read/34144329/amyloid-%C3%AE-1-42-peptide-induces-rapid-nmda-receptor-dependent-alterations-at-glutamatergic-synapses-in-the-entorhinal-cortex
#36
JOURNAL ARTICLE
Olayemi Joseph Olajide, Clifton Andrew Chapman
The hippocampus and entorhinal cortex (EC) accumulate amyloid beta peptides (Aβ) that promote neuropathology in Alzheimer's disease, but the early effects of Aβ on excitatory synaptic transmission in the EC have not been well characterized. To assess the acute effects of Aβ1-42 on glutamatergic synapses, acute brain slices from wildtype rats were exposed to Aβ1-42 or control solution for 3 hours, and tissue was analyzed using protein immunoblotting and quantitative PCR. Presynaptically, Aβ1-42 induced marked reductions in synaptophysin, synapsin-2a mRNA, and mGluR3 mRNA, and increased both VGluT2 protein and Ca2+ -activated channel KCa2...
September 2021: Neurobiology of Aging
https://read.qxmd.com/read/34110073/sex-specific-role-for-prefrontal-cortical-protein-interacting-with-c-kinase-1-in-cue-induced-cocaine-seeking
#37
JOURNAL ARTICLE
Megan M Wickens, Julia M Kirkland, Melissa C Knouse, Anna G McGrath, Lisa A Briand
Disruption of prefrontal glutamate receptor interacting protein (GRIP), which anchors GluA2-containing AMPA receptors (AMPARs) into the synaptic membrane, potentiates cue-induced cocaine seeking in both males and females. Protein interacting with C kinase 1 (PICK1) plays an opposing role to that of GRIP, removing AMPARs from the synapse. Consistent with our hypothesis that disruption of PICK1 in the mPFC would lead to a decrease in addiction-like behaviour, we found that conditional deletion of PICK1 in the mPFC attenuates cue-induced cocaine seeking in male mice...
September 2021: Addiction Biology
https://read.qxmd.com/read/34012961/the-arp2-3-inhibitory-protein-arpin-is-required-for-intestinal-epithelial-barrier-integrity
#38
JOURNAL ARTICLE
Sandra Chánez-Paredes, Armando Montoya-García, Karla F Castro-Ochoa, Julio García-Cordero, Leticia Cedillo-Barrón, Mineko Shibayama, Porfirio Nava, Sven Flemming, Nicolas Schlegel, Alexis M Gautreau, Hilda Vargas-Robles, Ricardo Mondragón-Flores, Michael Schnoor
The intestinal epithelial barrier (IEB) depends on stable interepithelial protein complexes such as tight junctions (TJ), adherens junctions (AJ), and the actin cytoskeleton. During inflammation, the IEB is compromised due to TJ protein internalization and actin remodeling. An important actin regulator is the actin-related protein 2/3 (Arp2/3) complex, which induces actin branching. Activation of Arp2/3 by nucleation-promoting factors is required for the formation of epithelial monolayers, but little is known about the relevance of Arp2/3 inhibition and endogenous Arp2/3 inhibitory proteins for IEB regulation...
2021: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/33967557/depletion-of-mrpl35-inhibits-gastric-carcinoma-cell-proliferation-by-regulating-downstream-signaling-proteins
#39
JOURNAL ARTICLE
Ling Yuan, Jia-Xin Li, Yi Yang, Yan Chen, Ting-Ting Ma, Shuang Liang, Yang Bu, Lei Yu, Yi Nan
BACKGROUND: Gastric carcinoma (GC) is a digestive system disease with high morbidity and mortality. However, early clinical detection is difficult, and the therapeutic effect for advanced disease is not satisfactory. Thus, finding new tumor markers and therapeutic targets conducive to the treatment of GC is imperative. MRPL35 is a member of the large subunit family of mitochondrial ribosomal protein. MRPL35 shows the characteristic of oncogene in colorectal cancer and esophageal cancer, which promotes the exploration of the correlation between MRPL35 and GC...
April 28, 2021: World Journal of Gastroenterology: WJG
https://read.qxmd.com/read/33946313/liposomal-encapsulated-fsc231-a-pick1-inhibitor-prevents-the-ischemia-reperfusion-induced-degradation-of-glua2-containing-ampa-receptors
#40
JOURNAL ARTICLE
Lindsay M Achzet, Fanny Astruc-Diaz, Phillip H Beske, Nicholas R Natale, Travis T Denton, Darrell A Jackson
Strokes remain one of the leading causes of disability within the United States. Despite an enormous amount of research effort within the scientific community, very few therapeutics are available for stroke patients. Cytotoxic accumulation of intracellular calcium is a well-studied phenomenon that occurs following ischemic stroke. This intracellular calcium overload results from excessive release of the excitatory neurotransmitter glutamate, a process known as excitotoxicity. Calcium-permeable AMPA receptors (AMPARs), lacking the GluA2 subunit, contribute to calcium cytotoxicity and subsequent neuronal death...
April 30, 2021: Pharmaceutics
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