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https://www.readbyqxmd.com/read/27902473/rituximab-conjugated-doxorubicin-loaded-microbubbles-as-a-theranostic-modality-in-b-cell-lymphoma
#1
Shoubing Zhou, Xiu Zhang, Cailian Wang
This study evaluated rituximab-conjugated, doxorubicin-loaded microbubbles (RDMs) in combination with ultrasound as molecular imaging agents for early diagnosis of B cell lymphomas, and as a targeted drug delivery system. Rituximab, a monoclonal CD20 antibody, was attached to the surfaces of doxorubicin-loaded microbubbles. RDM binding to B cell lymphoma cells was assessed using immunofluorescence. The cytotoxic effects of RDMs in combination with ultrasound (RDMs+US) were evaluated in vitro in CD20+ and CD20- cell lines, and its antitumor activities were assessed in Raji (CD20+) and Jurkat (CD20-) lymphoma cell-grafted mice...
November 25, 2016: Oncotarget
https://www.readbyqxmd.com/read/27887763/composite-lymphoma-with-diffuse-large-b-cell-lymphoma-and-classical-hodgkin-lymphoma-components-a-case-report-and-review-of-the-literature
#2
Gaurav Goyal, Austin Huy Nguyen, Kayla Kendric, Gabriel C Caponetti
Composite lymphoma (CL) is an infrequently diagnosed entity in which two or more distinct types of lymphomas occur synchronously in the same organ or anatomical site. Most commonly, CLs are composed of two non-Hodgkin B-cell lymphomas. We present a case of a composite lymphoma with diffuse large B-cell lymphoma, not otherwise specified (DLBCL-NOS) and classical Hodgkin lymphoma (CHL) components involving the terminal ileum, colon and pericolic lymph nodes. Immunohistochemical evaluation for determination of cell of origin of the DLBCL-NOS component indicated a germinal center B-cell subtype...
November 5, 2016: Pathology, Research and Practice
https://www.readbyqxmd.com/read/27886550/v%C3%AE-1-%C3%AE-%C3%AE-t-early-cardiac-infiltrated-innate-population-dominantly-producing-il-4-protect-mice-against-cvb3-myocarditis-by-modulating-ifn%C3%AE-t-response
#3
Fangfang Wan, Kepeng Yan, Dan Xu, Qian Qian, Hui Liu, Min Li, Wei Xu
Viral myocarditis (VMC) is an inflammation of the myocardium closely associated with Coxsackievirus B3 (CVB3) infection. Vγ1(+)γδT cells, one of early cardiac infiltrated innate population, were reported to protect CVB3 myocarditis while the precise mechanism not fully addressed. To explore cytokine profiles and kinetics of Vγ1(+)γδT and mechanism of protection against VMC, flow cytometry was conducted on cardiac Vγ1 cells in C57BL/6 mice following CVB3 infection. The level of cardiac inflammation, transthoracic echocardiography and viral replication were evaluated after monoclonal antibody depletion of Vγ1γδT...
November 22, 2016: Molecular Immunology
https://www.readbyqxmd.com/read/27885684/interference-free-mass-spectrometric-detection-of-capillary-isoelectric-focused-proteins-including-charge-variants-of-a-model-monoclonal-antibody
#4
Jens Hühner, Kevin Jooß, Neusüß Christian
CIEF represents an elegant technique especially for the separation of structural similar analytes, whereas MS is a state of the art instrumentation for the identification and characterization of biomolecules. The combination of both techniques can be realized by hyphenating CIEF with CZE-ESI-MS applying a mechanical valve. During the CZE step, the remaining ESI-interfering components of the CIEF electrolyte are separated from the analytes prior to MS detection. In this work, a multiple heart-cut approach is presented expanding our previous single heart-cut concept resulting in a dramatical reduction of analysis time...
November 25, 2016: Electrophoresis
https://www.readbyqxmd.com/read/27875559/efficacy-and-safety-of-anti-interleukin-5-therapy-in-patients-with-asthma-a-systematic-review-and-meta-analysis
#5
Fa-Ping Wang, Ting Liu, Zhu Lan, Su-Yun Li, Hui Mao
BACKGROUND: Recent trials have assessed the efficacy and safety of novel monoclonal antibodies such as reslizumab and benralizumab. However, the overall efficacy and safety anti-interleukin (IL) 5 treatment in asthma have not been thoroughly assessed. METHODS: Randomized controlled trials (RCTs) of anti-IL-5 treatment on patients with asthma published up to October 2016 in PubMed, Embase, and Cochrane Central Register of Controlled Trials (CENTRAL) that reported pulmonary function, quality of life scores, asthmatic exacerbation rate, blood and sputum eosinophil counts, short-acting β-agonist (SABA) rescue use, and adverse events were included...
2016: PloS One
https://www.readbyqxmd.com/read/27852589/effects-of-the-p-selectin-antagonist-inclacumab-on-myocardial-damage-after-percutaneous-coronary-intervention-according-to-timing-of-infusion-insights-from-the-select-acs-trial
#6
Barbara E Stähli, Catherine Gebhard, Valérie Duchatelle, Daniel Cournoyer, Thibaut Petroni, Jean-François Tanguay, Stephen Robb, Jessica Mann, Marie-Claude Guertin, R Scott Wright, Philippe L L'Allier, Jean-Claude Tardif
BACKGROUND: The Effects of the P-Selectin Antagonist Inclacumab on Myocardial Damage After Percutaneous Coronary Intervention for Non-ST-Segment Elevation Myocardial Infarction (SELECT-ACS) trial suggested beneficial effects of inclacumab, a monoclonal antibody directed against P-selectin, on periprocedural myocardial damage. This study evaluated the effect of inclacumab on myocardial damage according to varying time intervals between study drug infusion and percutaneous coronary intervention (PCI)...
November 16, 2016: Journal of the American Heart Association
https://www.readbyqxmd.com/read/27824651/infliximab-selectively-modulates-the-circulating-blood-monocyte-repertoire-in-crohn-s-disease
#7
Stephanie M Slevin, Michael Conall Dennedy, Eanna P Connaughton, Andreia Ribeiro, Rhodri Ceredig, Matthew D Griffin, Laurence J Egan
BACKGROUND: Infliximab (IFX), an anti-tumour necrosis factor alpha (TNFα) monoclonal antibody, provides clinical benefits in treating Crohn's disease (CD) but its mechanisms of action are not fully elucidated. This study investigated blood monocyte repertoires and the acute effects of IFX infusion on monocyte subset phenotype and function in IFX-treated patients with CD. METHODS: Monocytes and monocyte subsets were enumerated and phenotypically characterized by multicolor flow cytometry in freshly isolated blood from healthy controls (n = 21) and patients with CD treated with (IFX, n = 24) and without (non-IFX, n = 20) IFX...
December 2016: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/27802880/surface-induced-three-peak-elution-behavior-of-a-monoclonal-antibody-during-cation-exchange-chromatography
#8
Jing Guo, Arch D Creasy, Gregory Barker, Giorgio Carta
A monoclonal antibody exhibits a two- or three-peak elution behavior when loaded on the CEX resin POROS XS and eluted with a salt gradient. Two peaks are observed without a hold step while a third more strongly retained peak becomes noticeable with a hold time as low as 10min. As the hold time is increased further, the first peak gradually disappears, the second peak initially increases and then decreases, and the third peak continuously increases. Dynamic light scattering shows that the third peak contains significant levels of aggregates formed in the column...
October 26, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27795368/safety-tolerability-and-pharmacokinetics-of-medi4893-an-investigational-extendedhalf-life-anti-staphylococcus-aureus-alpha-toxin-human-monoclonal-antibody-in-healthy-adults
#9
Xiang-Qing Yu, Gabriel J Robbie, Yuling Wu, Mark T Esser, Kathryn Jensen, Howard I Schwartz, Terramika Bellamy, Martha Hernandez-Illas, Hasan S Jafri
MEDI4893 is an investigational immunoglobulin G1K monoclonal antibody that specifically binds to and neutralizes alpha-toxin, a key Staphylococcus aureus virulence factor. A triple-amino acid substitution, M252Y/S254T/T256E, was engineered into the MEDI4893 Fc region to extend its serum half-life. A phase 1, double-blind, dose escalation study was designed to evaluate the safety, tolerability, pharmacokinetics, anti-alpha-toxin neutralizing activity, and anti-drug antibody (ADA) response of MEDI4893 following a single intravenous infusion in healthy adults 18-65 years of age...
October 17, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27794039/visualization-of-dna-g-quadruplexes-in-herpes-simplex-virus-1-infected-cells
#10
Sara Artusi, Rosalba Perrone, Sara Lago, Paolo Raffa, Enzo Di Iorio, Giorgio Palù, Sara N Richter
We have previously shown that clusters of guanine quadruplex (G4) structures can form in the human herpes simplex-1 (HSV-1) genome. Here we used immunofluorescence and immune-electron microscopy with a G4-specific monoclonal antibody to visualize G4 structures in HSV-1 infected cells. We found that G4 formation and localization within the cells was virus cycle dependent: viral G4s peaked at the time of viral DNA replication in the cell nucleus, moved to the nuclear membrane at the time of virus nuclear egress and were later found in HSV-1 immature virions released from the cell nucleus...
October 27, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27754462/toxicity-and-growth-assessments-of-three-thermophilic-benthic-dinoflagellates-ostreopsis-cf-ovata-prorocentrum-lima-and-coolia-monotis-developing-in-the-southern-mediterranean-basin
#11
Hela Ben-Gharbia, Ons Kéfi-Daly Yahia, Zouher Amzil, Nicolas Chomérat, Eric Abadie, Estelle Masseret, Manoella Sibat, Habiba Zmerli Triki, Habiba Nouri, Mohamed Laabir
Harmful benthic dinoflagellates, usually developing in tropical areas, are expanding to temperate ecosystems facing water warming. Reports on harmful benthic species are particularly scarce in the Southern Mediterranean Sea. For the first time, three thermophilic benthic dinoflagellates (Ostreopsis cf. ovata, Prorocentrum lima and Coolia monotis) were isolated from Bizerte Bay (Tunisia, Mediterranean) and monoclonal cultures established. The ribotyping confirmed the morphological identification of the three species...
October 15, 2016: Toxins
https://www.readbyqxmd.com/read/27722854/disease-drug-interaction-of-sarilumab-and-simvastatin-in-patients-with-rheumatoid-arthritis
#12
Eun Bong Lee, Nikki Daskalakis, Christine Xu, Anne Paccaly, Barry Miller, Roy Fleischmann, Inga Bodrug, Alan Kivitz
INTRODUCTION: Elevated interleukin (IL)-6 occurs in patients with active rheumatoid arthritis (RA), which has been shown to lead to a decrease in cytochrome P450 (CYP) enzyme activity and alterations in drug concentrations metabolized by CYP. IL-6 signaling blockade by IL-6 receptor (IL-6R) antagonists may reverse this effect of IL-6 and restore CYP activity. This study evaluated the pharmacokinetic profile of simvastatin (a CYP3A4 substrate) before and 1 week after a single dose of sarilumab (a human monoclonal antibody [mAb] blocking the IL-6Rα) in patients with RA, to assess potential interaction...
October 8, 2016: Clinical Pharmacokinetics
https://www.readbyqxmd.com/read/27715399/proteolytic-fragments-of-fibronectin-function-as-matrikines-driving-the-chemotactic-affinity-of-prostate-cancer-cells-to-human-bone-marrow-mesenchymal-stromal-cells-via-the-%C3%AE-5%C3%AE-1-integrin
#13
Raghav Joshi, Edi Goihberg, Wenying Ren, Monika Pilichowska, Paul Mathew
: The haematopoietic niche is contributed to by bone marrow-resident mesenchymal stromal cells (BM-MSCs) and subverted by prostate cancer cells. To study mechanisms by which BM-MSCs and prostate cancer cells may interact, we assessed the migration, invasion, adhesion and proliferation of bone-derived prostate cancer cells (PC-3) in co-culture with pluripotent human BM-MSCs. We observed a strong adhesive, migratory and invasive phenotype of PC-3 cells with BM- MSC-co-culture and set out to isolate and characterize the bioactive principle...
August 11, 2016: Cell Adhesion & Migration
https://www.readbyqxmd.com/read/27700008/translational-pharmacokinetic-pharmacodynamic-analysis-of-myo-029-antibody-for-muscular-dystrophy
#14
P Singh, H Rong, T Gordi, J Bosley, I Bhattacharya
Suppression of the myostatin (GDF-8) pathway has emerged as an important therapeutic paradigm for muscle-wasting disorders. In this study, we conducted a translational pharmacokinetic/pharmacodynamic (PK/PD) analysis of MYO-029, an anti-myostatin monoclonal antibody, using PK data in mice, rats, monkeys, humans, mouse tissue distribution data with (125) I-labeled MYO-029, muscle weight increase in SCID mice, and muscle circumference changes in monkeys. This analysis revealed significant in vivo potency shift between mice and monkeys (72 nM vs...
October 4, 2016: Clinical and Translational Science
https://www.readbyqxmd.com/read/27660109/choosing-the-right-protein-a-affinity-chromatography-media-can-remove-aggregates-efficiently
#15
Tomokazu Yada, Koichi Nonaka, Masayuki Yabuta, Noriko Yoshimoto, Shuichi Yamamoto
Protein A chromatography (PAC) is commonly used as an efficient capture step in monoclonal antibody (mAb) separation processes. Usually dynamic binding capacity is used for choosing the right PAC. However, if aggregates can be efficiently removed during elution, it can make the following polishing steps easier. In this study a method for choosing the right PAC media in terms of mAb aggregate removal is proposed. Linear pH gradient elution experiments of two different mAbs on various PAC columns are carried out, where the elution behavior of aggregates as well as the monomer is measured...
September 23, 2016: Biotechnology Journal
https://www.readbyqxmd.com/read/27645784/antibody-prophylaxis-against-dengue-virus-2-infection-in-non-human-primates
#16
Monika Simmons, Robert Putnak, Peifang Sun, Timothy Burgess, Wayne A Marasco
Passive immunization with anti-dengue virus (DENV) immune serum globulin (ISG) or monoclonal antibodies (Mabs) may serve to supplement or replace vaccination for short-term dengue immune prophylaxis. In the present study, we sought to establish proof-of-concept by evaluating several DENV-neutralizing antibodies for their ability to protect rhesus macaques against viremia following live virus challenge, including human anti-dengue ISG, and a human Mab (Mab11/wt) and its genetically engineered variant (Mab11/mutFc) that is unable to bind to cells with Fc gamma receptors (FcγR) and potentiate antibody-dependent enhancement (ADE)...
November 2, 2016: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/27642033/a-combination-hepatoma-targeted-therapy-based-on-nanotechnology-phre-egr1-hsv-tk-131-i-antiafpmcab-gcv-mfh
#17
Mei Lin, Junxing Huang, Xingmao Jiang, Jia Zhang, Hong Yu, Jun Ye, Dongsheng Zhang
Combination targeted therapy is a promising cancer therapeutic strategy. Here, using PEI-Mn0.5Zn0.5Fe2O4 nanoparticles (PEI-MZF-NPs) as magnetic media for MFH (magnetic fluid hyperthermia) and gene transfer vector for gene-therapy, a combined therapy, pHRE-Egr1-HSV-TK/(131)I-antiAFPMcAb-GCV/MFH, for hepatoma is developed. AntiAFPMcAb (Monoclonal antibody AFP) is exploited for targeting. The plasmids pHRE-Egr1-HSV-TK are achieved by incorporation of pEgr1-HSV-TK and pHRE-Egr1-EGFP. Restriction enzyme digestion and PCR confirm the recombinant plasmids pHRE-Egr1-HSV-TK are successfully constructed...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27632937/use-of-oral-fluids-for-detection-of-virus-and-antibodies-in-pigs-infected-with-swine-vesicular-disease-virus
#18
C Senthilkumaran, H Bittner, A Ambagala, O Lung, S Babiuk, M Yang, J Zimmerman, L G Giménez-Lirola, C Nfon
The use of swine oral fluid (OF) for the detection of nucleic acids and antibodies is gaining significant popularity. Assays have been developed for this purpose for endemic and foreign animal diseases of swine. Here, we report the use of OF for the detection of virus and antibodies in pigs experimentally infected with swine vesicular disease virus (SVDV), a virus that causes a disease clinically indistinguishable from the economically devastating foot-and-mouth disease. Viral genome was detected in OF by real-time reverse transcription polymerase chain reaction (RRT-PCR) from 1 day post-infection (DPI) to 21 DPI...
September 15, 2016: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/27632364/multi-dose-romidepsin-reactivates-replication-competent-siv-in-post-antiretroviral-rhesus-macaque-controllers
#19
Benjamin B Policicchio, Cuiling Xu, Egidio Brocca-Cofano, Kevin D Raehtz, Tianyu He, Dongzhu Ma, Hui Li, Ranjit Sivanandham, George S Haret-Richter, Tammy Dunsmore, Anita Trichel, John W Mellors, Beatrice H Hahn, George M Shaw, Ruy M Ribeiro, Ivona Pandrea, Cristian Apetrei
Viruses that persist despite seemingly effective antiretroviral treatment (ART) and can reinitiate infection if treatment is stopped preclude definitive treatment of HIV-1 infected individuals, requiring lifelong ART. Among strategies proposed for targeting these viral reservoirs, the premise of the "shock and kill" strategy is to induce expression of latent proviruses [for example with histone deacetylase inhibitors (HDACis)] resulting in elimination of the affected cells through viral cytolysis or immune clearance mechanisms...
September 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27570560/cd146-targeted-immunopet-and-nirf-imaging-of-hepatocellular-carcinoma-with-a-dual-labeled-monoclonal-antibody
#20
Reinier Hernandez, Haiyan Sun, Christopher G England, Hector F Valdovinos, Emily B Ehlerding, Todd E Barnhart, Yunan Yang, Weibo Cai
Overexpression of CD146 has been correlated with aggressiveness, recurrence rate, and poor overall survival in hepatocellular carcinoma (HCC) patients. In this study, we set out to develop a CD146-targeting probe for high-contrast noninvasive in vivo positron emission tomography (PET) and near-infrared fluorescence (NIRF) imaging of HCCs. YY146, an anti-CD146 monoclonal antibody, was employed as a targeting molecule to which we conjugated the zwitterionic near-infrared fluorescence (NIRF) dye ZW800-1 and the chelator deferoxamine (Df)...
2016: Theranostics
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