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https://www.readbyqxmd.com/read/28297588/oncostatin-m-preconditioned-mesenchymal-stem-cells-alleviate-bleomycin-induced-pulmonary-fibrosis-through-paracrine-effects-of-the-hepatocyte-growth-factor
#1
Ying-Wei Lan, Si-Min Theng, Tsung-Teng Huang, Kong-Bung Choo, Chuan-Mu Chen, Han-Pin Kuo, Kowit-Yu Chong
Mesenchymal stem cells (MSCs) are widely considered for treatment of pulmonary fibrosis based on the anti-inflammatory, antifibrotic, antiapoptotic, and regenerative properties of the cells. Recently, elevated levels of oncostatin M (OSM) have been reported in the bronchoalveolar lavage fluid of a pulmonary fibrosis animal model and in patients. In this work, we aimed to prolong engrafted MSC survival and to enhance the effectiveness of pulmonary fibrosis transplantation therapy by using OSM-preconditioned MSCs...
March 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28290674/copper-i-phosphine-polypyridyl-complexes-synthesis-characterization-dna-hsa-binding-study-and-antiproliferative-activity
#2
Wilmer Villarreal, Legna Colina-Vegas, Gonzalo Visbal, Oscar Corona, Rodrigo S Corrêa, Javier Ellena, Marcia Regina Cominetti, Alzir Azevedo Batista, Maribel Navarro
A series of copper(I)-phosphine polypyridyl complexes have been investigated as potential antitumor agents. The complexes [Cu(PPh3)2dpq]NO3 (2), [Cu(PPh3)2dppz]NO3 (3), [Cu(PPh3)2dppa]NO3 (4), and [Cu(PPh3)2dppme]NO3 (5) were synthesized by the reaction of [Cu(PPh3)2NO3] with the respective planar ligand under mild conditions. These copper complexes were fully characterized by elemental analysis, molar conductivity, FAB-MS, and NMR, UV-vis, and IR spectroscopies. Interactions between these copper(I)-phosphine polypyridyl complexes and DNA have been investigated using various spectroscopic techniques and analytical methods, such as UV-vis titrations, thermal denaturation, circular dichroism, viscosity measurements, gel electrophoresis, and competitive fluorescent intercalator displacement assays...
March 14, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28289860/the-pd-l1-pd-1-pathway-promotes-dysfunction-but-not-exhaustion-in-tumor-responding-t-cells-from-pleural-effusions-in-lung-cancer-patients
#3
Heriberto Prado-Garcia, Susana Romero-Garcia, Alejandra Puerto-Aquino, Uriel Rumbo-Nava
Malignant pleural effusions are frequent in patients with advanced stages of lung cancer and are commonly infiltrated by lymphocytes and tumor cells. CD8+ T cells from these effusions have reduced effector functions. The programmed death receptor 1(PD-1)/programmed death ligand 1 (PD-L1) pathway is involved in T-cell exhaustion, and it might be responsible for T-cell dysfunction in lung cancer patients. Here, we show that PD-L1 is expressed on tumor cell samples from malignant effusions, on lung cancer cell lines, and, interestingly, on MRC-5 lung fibroblasts...
March 13, 2017: Cancer Immunology, Immunotherapy: CII
https://www.readbyqxmd.com/read/28282127/revisiting-previously-investigated-plants-a-molecular-networking-based-study-of-geissospermum-laeve
#4
Alexander E Fox Ramos, Charlotte Alcover, Laurent Evanno, Alexandre Maciuk, Marc Litaudon, Christophe Duplais, Guillaume Bernadat, Jean-François Gallard, Jean-Christophe Jullian, Elisabeth Mouray, Philippe Grellier, Philippe M Loiseau, Sébastien Pomel, Erwan Poupon, Pierre Champy, Mehdi A Beniddir
Three new monoterpene indole alkaloids (1-3) have been isolated from the bark of Geissospermum laeve, together with the known alkaloids (-)-leuconolam (4), geissolosimine (5), and geissospermine (6). The structures of 1-3 were elucidated by analysis of their HRMS and NMR spectroscopic data. The absolute configuration of geissolaevine (1) was deduced from the comparison of experimental and theoretically calculated ECD spectra. The isolation workflow was guided by a molecular networking-based dereplication strategy using an in-house database of monoterpene indole alkaloids...
March 10, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28258769/cytotoxicity-and-genotoxicity-of-a-low-shrinkage-monomer-and-monoacylphosphine-oxide-photoinitiator-comparative-analyses-of-individual-toxicity-and-combination-effects-in-mixtures
#5
Dragica Manojlovic, Miroslav D Dramićanin, Vesna Miletic, Dragana Mitić-Ćulafić, Bojana Jovanović, Biljana Nikolić
OBJECTIVE: To compare cytotoxicity and genotoxicity of novel urethane-based monomer FIT-852 and monoacylphosphine oxide photoinitiator (Lucirin TPO) with conventional Bisphenol A-glycidyl-methacrylate (BisGMA) and triethylene glycol dimethacrylate (TEGDMA) monomers and camphorquinone (CQ)/amine photoinitiator system, respectively. Moreover, we quantified and analyzed the combinatorial effects of individual substances in resin-based mixtures concerning the nature of the combinatorial effects...
February 28, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28243638/interstrand-dna-covalent-binding-of-two-dinuclear-ru-ii-complexes-influence-of-the-extra-ring-of-the-bridging-ligand-on-the-dna-interaction-and-cytotoxic-activity
#6
Héctor J Lozano, Natalia Busto, Gustavo Espino, Arancha Carbayo, José M Leal, James A Platts, Begoña García
In this work, we report experimental and computational evidence for the intercalation into the DNA base-pairs of the free quinones quinizarin (Q) and naphthazarin (N) and the interstrand covalent binding of their p-cymene di-ruthenium(ii) complexes (Cl2Ru2X, with X = N, Q bridging ligands). The intercalation extent for the N complex was larger than that for Q, which is in good agreement with the higher relative contour length and melting temperature for the same CX/CDNA ratio and with the computational mean stacking distances between the ligand and the nearest base-pair (3...
March 14, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28223069/evaluation-of-antibacterial-potential-and-toxicity-of-plant-volatile-compounds-using-new-broth-microdilution-volatilization-method-and-modified-mtt-assay
#7
Marketa Houdkova, Johana Rondevaldova, Ivo Doskocil, Ladislav Kokoska
With aim to develop effective proof-of-concept approach which can be used in a development of new preparations for the inhalation therapy, we designed a new screening method for simple and rapid simultaneous determination of antibacterial potential of plant volatiles in the liquid and the vapour phase at different concentrations. In addition, EVA (ethylene vinyl acetate) capmat™ as vapour barrier cover was used as reliable modification of thiazolyl blue tetrazolium bromide (MTT) assay for cytotoxicity testing of volatiles on microtiter plates...
February 20, 2017: Fitoterapia
https://www.readbyqxmd.com/read/28203673/amphipathic-guanidine-embedded-glyoxamide-based-peptidomimetics-as-novel-antibacterial-agents-and-biofilm-disruptors
#8
Shashidhar Nizalapur, Onder Kimyon, Eugene Yee, Kitty Ho, Thomas Berry, Mike Manefield, Charles G Cranfield, Mark Willcox, David StC Black, Naresh Kumar
Antimicrobial resistance in bacteria is becoming increasingly prevalent, posing a critical challenge to global health. Bacterial biofilm formation is a common resistance mechanism that reduces the effectiveness of antibiotics. Thus, the development of compounds that can disrupt bacterial biofilms is a potential strategy to combat antimicrobial resistance. We report herein the synthesis of amphipathic guanidine-embedded glyoxamide-based peptidomimetics via ring-opening reactions of N-naphthoylisatins with amines and amino acids...
February 16, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28192961/raman-and-infrared-spectroscopy-distinguish-replicative-senescent-from-proliferating-primary-human-fibroblast-cells-by-detecting-spectral-differences-mainly-due-to-biomolecular-alterations
#9
Katharina Eberhardt, Claudia Beleites, Shiva Marthandan, Christian Matthäus, Stephan Diekmann, Juergen Popp
Cellular senescence is a terminal cell cycle arrested state, assumed to be involved in tumor suppression. We studied four human fibroblast cell strains (BJ, MRC 5, IMR 90 and WI 38) from proliferation into senescence. Cells were investigated by label-free vibrational Raman and infrared spectroscopy, following their transition into replicative senescence. During the transition into senescence, we observed rather similar biomolecular abundances in all four cell strains and between proliferating and senescent cells; however, in the four aging cell strains we found common molecular differences dominated by protein and lipid modifications...
January 27, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28173977/identification-of-a-candidate-standard-strain-of-severe-fever-with-thrombocytopenia-syndrome-virus-for-vaccine-quality-control-in-china-using-a-cross-neutralization-assay
#10
Zheng Jia, Xiaohong Wu, Ling Wang, Xiuling Li, Xinxian Dai, Mifang Liang, Shouchun Cao, Yan Kong, Jingjing Liu, Yuhua Li, Junzhi Wang
Severe fever with thrombocytopenia syndrome (SFTS) is caused by a phlebovirus of the Bunyaviridae family, which is designated as SFTS virus (SFTSV). To our knowledge, no efficient SFTSV vaccine exists. Here, we report the identification of a standard virus strain for the eight major SFTSV strains circulating in China for use in evaluating the SFTSV vaccine. Rabbits were immunized with the SFTSV strains and the cross-neutralization capacities of SFTSV anti-sera were determined in microculture cytopathic effect (CPE)-inhibition assays...
February 4, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28161627/temperature-and-ph-sensors-based-on-graphenic-materials
#11
P Salvo, N Calisi, B Melai, B Cortigiani, M Mannini, A Caneschi, G Lorenzetti, C Paoletti, T Lomonaco, A Paolicchi, I Scataglini, V Dini, M Romanelli, R Fuoco, F Di Francesco
Point-of-care applications and patients' real-time monitoring outside a clinical setting would require disposable and durable sensors to provide better therapies and quality of life for patients. This paper describes the fabrication and performances of a temperature and a pH sensor on a biocompatible and wearable board for healthcare applications. The temperature sensor was based on a reduced graphene oxide (rGO) layer that changed its electrical resistivity with the temperature. When tested in a human serum sample between 25 and 43°C, the sensor had a sensitivity of 110±10Ω/°C and an error of 0...
May 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28145721/isolation-and-total-synthesis-of-hoshinolactam-an-antitrypanosomal-lactam-from-a-marine-cyanobacterium
#12
Hidetoshi Ogawa, Arihiro Iwasaki, Shimpei Sumimoto, Masato Iwatsuki, Aki Ishiyama, Rei Hokari, Kazuhiko Otoguro, Satoshi O Mura, Kiyotake Suenaga
In the search for new antiprotozoal substances, hoshinolactam, an antitrypanosomal lactam, was isolated from a marine cyanobacterium. The gross structure was elucidated by spectroscopic analyses, and the absolute configuration was determined by the first total synthesis. Hoshinolactam showed potent antitrypanosomal activity with an IC50 value of 3.9 nM without cytotoxicity against human fetal lung fibroblast MRC-5 cells (IC50 > 25 μM).
February 1, 2017: Organic Letters
https://www.readbyqxmd.com/read/28135634/design-synthesis-and-in%C3%A2-vitro-antitumour-activity-of-new-goniofufurone-and-7-epi-goniofufurone-mimics-with-halogen-or-azido-groups-at-the-c-7-position
#13
Jovana Francuz, Ivana Kovačević, Mirjana Popsavin, Goran Benedeković, Bojana Srećo Zelenović, Vesna Kojić, Dimitar Jakimov, Lidija Aleksić, Gordana Bogdanović, Tatjana Srdić-Rajić, Eva Lončar, Marko V Rodić, Vladimir Divjaković, Velimir Popsavin
A series of new antitumour lactones containing the [3.3.0] bicyclic furano-lactone core and the halogen or azido group at the C-7 position have been designed, synthesized, and evaluated for their in vitro antitumour activity against a panel of human tumour cell lines. Some of the analogues displayed powerful antiproliferative effects to certain human tumour cells, but all of them were devoid of any cytotoxicity towards the normal foetal lung fibroblasts (MRC-5). A SAR study reveals the structural features of these lactones that may affect their antiproliferative activity...
January 17, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28124843/improved-antioxidative-and-cytotoxic-activities-of-chamomile-matricaria-chamomilla-florets-fermented-by-lactobacillus-plantarum-kccm-11613p
#14
Eun-Hye Park, Won-Young Bae, Su-Jin Eom, Kee-Tae Kim, Hyun-Dong Paik
Antioxidative and cytotoxic effects of chamomile (Matricaria chamomilla) fermented by Lactobacillus plantarum were investigated to improve their biofunctional activities. Total polyphenol (TP) content was measured by the Folin-Denis method, and the antioxidant activities were assessed by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and β-carotene bleaching method. AGS, HeLa, LoVo, MCF-7, and MRC-5 (normal) cells were used to examine the cytotoxic effects by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28122968/phosphoprotein-profiles-of-candidate-markers-for-early-cellular-responses-to-low-dose-%C3%AE-radiation-in-normal-human-fibroblast-cells
#15
Ji-Hye Yim, Jung Mi Yun, Ji Young Kim, In Kyung Lee, Seon Young Nam, Cha Soon Kim
Ionizing radiation causes biological damage that leads to severe health effects. However, the effects and subsequent health implications caused by exposure to low-dose radiation are unclear. The objective of this study was to determine phosphoprotein profiles in normal human fibroblast cell lines in response to low-dose and high-dose γ-radiation. We examined the cellular response in MRC-5 cells 0.5 h after exposure to 0.05 or 2 Gy. Using 1318 antibodies by antibody array, we observed ≥1.3-fold increases in a number of identified phosphoproteins in cells subjected to low-dose (0...
January 24, 2017: Journal of Radiation Research
https://www.readbyqxmd.com/read/28122543/lung-cells-support-osteosarcoma-cell-migration-and-survival
#16
Shibing Yu, Mitchell Stephen Fourman, Adel Mahjoub, Jonathan Brendan Mandell, Jared Anthony Crasto, Nicholas Giuseppe Greco, Kurt Richard Weiss
BACKGROUND: Osteosarcoma (OS) is the most common primary bone tumor, with a propensity to metastasize to the lungs. Five-year survival for metastatic OS is below 30%, and has not improved for several decades despite the introduction of multi-agent chemotherapy. Understanding OS cell migration to the lungs requires an evaluation of the lung microenvironment. Here we utilized an in vitro lung cell and OS cell co-culture model to explore the interactions between OS and lung cells, hypothesizing that lung cells would promote OS cell migration and survival...
January 25, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28117761/phloretin-exerts-anti-tuberculosis-activity-and-suppresses-lung-inflammation
#17
Dasom Jeon, Min-Cheol Jeong, Hum Nath Jnawali, Chulhee Kwak, Sungwon Ryoo, In Duk Jung, Yangmee Kim
An increase in the prevalence of the drug-resistant Mycobacteria tuberculosis necessitates developing new types of anti-tuberculosis drugs. Here, we found that phloretin, a naturally-occurring flavonoid, has anti-mycobacterial effects on H37Rv, multi-drug-, and extensively drug-resistant clinical isolates, with minimum inhibitory concentrations of 182 and 364 μM, respectively. Since Mycobacteria cause lung inflammation that contributes to tuberculosis pathogenesis, anti-inflammatory effects of phloretin in interferon-γ-stimulated MRC-5 human lung fibroblasts and lipopolysaccharide (LPS)-stimulated dendritic cells were investigated...
January 22, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28069192/acoustic-cavitation-induced-generation-of-stabilizer-free-extremely-stable-reduced-graphene-oxide-nanodispersion-for-efficient-delivery-of-paclitaxel-in-cancer-cells
#18
Renu Geetha Bai, Kasturi Muthoosamy, Fiona Natalia Shipton, Sivakumar Manickam
Graphene is one of the highly explored nanomaterials due to its unique and extraordinary properties. In this study, by utilizing a hydrothermal reduction method, graphene oxide (GO) was successfully converted to reduced graphene oxide (RGO) without using any toxic reducing agents. Following this, with the use of ultrasonic cavitation, profoundly stable few layer thick RGO nanodispersion was generated without employing any stabilizers or surfactants. During ultrasonication, shockwaves from the collapse of bubbles cause a higher dispersing energy to the graphene nanosheets which surpass the forces of Van der Waal's and π-π stacking and thus pave the way to form a stable aqueous nanodispersion of graphene...
May 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28069023/in-vitro-biological-responses-to-nanofibrillated-cellulose-by-human-dermal-lung-and-immune-cells-surface-chemistry-aspect
#19
Viviana R Lopes, Carla Sanchez-Martinez, Maria Strømme, Natalia Ferraz
BACKGROUND: Nanocellulose, and particularly nanofibrillated cellulose (NFC), has been proposed for a diversity of applications in industry and in the biomedical field. Its unique physicochemical and structural features distinguish nanocellulose from traditional materials and enable its use as an advance nanomaterial. However, its nanoscale features may induce unknown biological responses. Limited studies with NFC are available and the biological impacts of its use have not been thoroughly explored...
January 10, 2017: Particle and Fibre Toxicology
https://www.readbyqxmd.com/read/28056952/litchi-seed-extract-inhibits-epidermal-growth-factor-receptor-signaling-and-growth-of-two-non-small-cell-lung-carcinoma-cells
#20
Yuan-Chiang Chung, Chin-Hui Chen, Yu-Ting Tsai, Chih-Cheng Lin, Jyh-Ching Chou, Ting-Yu Kao, Chiu-Chen Huang, Chi-Hsuan Cheng, Chih-Ping Hsu
BACKGROUND: Litchi seeds possess rich amounts of phenolics and have been shown to inhibit proliferation of several types of cancer cells. However, the suppression of EGFR signaling in non-small cell lung cancer (NSCLC) by litchi seed extract (LCSE) has not been fully understood. METHODS: In this study, the effects of LCSE on EGFR signaling, cell proliferation, the cell cycle and apoptosis in A549 adenocarcinoma cells and NCI- H661 large-cell carcinoma cells were examined...
January 5, 2017: BMC Complementary and Alternative Medicine
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