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https://www.readbyqxmd.com/read/28408225/synthesis-of-isoflavene-thiosemicarbazone-hybrids-and-evaluation-of-their-anti-tumor-activity
#1
Eugene M H Yee, Miriam B Brandl, David StC Black, Orazio Vittorio, Naresh Kumar
Phenoxodiol is an isoflavene with potent anti-tumor activity. In this study, a series of novel mono- and di-substituted phenoxodiol-thiosemicarbazone hybrids were synthesized via the condensation reaction between phenoxodiol with thiosemicarbazides. The in vitro anti-proliferative activities of the hybrids were evaluated against the neuroblastoma SKN-BE(2)C, the triple negative breast cancer MDA-MB-231, and the glioblastoma U87 cancer cell lines. The mono-substituted hybrids exhibited potent anti-proliferative activity against all three cancer cell lines, while the di-substituted hybrids were less active...
April 3, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28405259/a549-and-mrc-5-cell-aggregation-in-a-microfluidic-lab-on-a-chip-system
#2
A Zuchowska, E Jastrzebska, K Zukowski, M Chudy, A Dybko, Z Brzozka
In this paper, we present a culture of A549 and MRC-5 spheroids in a microfluidic system. The aim of our work was to develop a good lung cancer model for the evaluation of drug cytotoxicity. Our research was focused on determining the progress of cell aggregation depending on such factors as the depth of culture microwells in the microdevices, a different flow rate of the introduced cell suspensions, and the addition of collagen to cell suspensions. We showed that these factors had a significant influence on spheroid formation...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28403652/a-contribution-to-pharmaceutical-biology-of-freshwater-sponges
#3
Aleksandra Talevska, Boris Pejin, Vesna Kojic, Tanja Beric, Slavisa Stankovic
In vitro anti-tumour and anti-radical activities of the acetone extract of the freshwater sponge Ochridaspongia rotunda were the subject of this study. The extract was found to be highly cytotoxic to human lung tumour cell line A-549 reaching IC50 value of 5.01 ± 0.21 μg/mL. Indeed, it displayed only 2-fold less anti-tumour activity than doxorubicin (IC50 value 2.42 ± 0.13 μg/mL) used as a positive control. The same extract was also found to be almost 37-fold more selective against A-549 vs. MRC-5 (normal) lung cells, in difference to weak selectivity of doxorubicin (less than 3-fold)...
April 13, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28393250/inhibition-of-varicella%C3%A2-zoster-virus-replication-by-an-ethanol-extract-of-lysimachia-mauritiana
#4
Sohee Bae, Yoon-Jae Song
Varicella‑zoster virus (VZV), a ubiquitous human α‑herpesvirus, is the causative agent of chickenpox and shingles. In the present study, we investigated the antiviral activity of a 70% ethanol extract of Lysimachia mauritiana (LME) against VZV. LME strongly interfered with the replication of both laboratory and clinical strains of VZV without affecting the viability of MRC‑5 cells. Moreover, LME treatment suppressed the expression of an essential viral transactivator, immediate‑early 62 protein (IE62), in addition to other lytic genes in the later phases of viral replication...
April 7, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28370894/antrodia-camphorata-attenuates-cigarette-smoke-induced-ros-production-dna-damage-apoptosis-and-inflammation-in-vascular-smooth-muscle-cells-and-atherosclerosis-in-apoe-deficient-mice
#5
Hsin-Ling Yang, Mallikarjuna Korivi, Cheng-Hsien Chen, Wei-Jung Peng, Chee-Shan Chen, Mei-Ling Li, Li-Sung Hsu, Jiunn-Wang Liao, You-Cheng Hseu
Cigarette smoke exposure activates several cellular mechanisms predisposing to atherosclerosis, including oxidative stress, dyslipidemia, and vascular inflammation. Antrodia camphorata, a renowned medicinal mushroom in Taiwan, has been investigated for its antioxidant, anti-inflammatory, and antiatherosclerotic properties in cigarette smoke extracts (CSE)-treated vascular smooth muscle cells (SMCs), and ApoE-deficient mice. Fermented culture broth of Antrodia camphorata (AC, 200-800 µg/mL) possesses effective antioxidant activity against CSE-induced ROS production...
April 3, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/28366842/promising-anticancer-activity-of-cyclotrichium-niveum-l-extracts-through-induction-of-both-apoptosis-and-necrosis
#6
Aysun Özdemir, Metin Yildiz, Fatma Sezer Senol, Yaprak Dilber Şimay, Burçin Ibişoglu, Alper Gokbulut, Ilkay Erdogan Orhan, Mustafa Ark
In this study, cytotoxic effects of the dichloromethane, ethyl acetate, ethanol, and aqueous extracts of the aerial parts of Cyclotrichium niveum (Boiss.) Manden. & Scheng (Lamiaceae) were evaluated. We tested HeLa, MCF-7 cancer cells, and MRC-5 and MCF-10A normal cells. The ethyl acetate and dichloromethane extracts induced cytotoxicity whereas the ethanol and aqueous extracts had no cytotoxic activity against both cancer cells. IC50 values of the dichloromethane extract were 353.0 ± 84.30 μg/ml, 114...
March 30, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28359304/determination-of-the-cell-permissiveness-spectrum-mode-of-rna-replication-and-rna-protein-interaction-of-zika-virus
#7
Wangheng Hou, Najealicka Armstrong, Lilian Akello Obwolo, Michael Thomas, Xiaowu Pang, Kevin S Jones, Qiyi Tang
BACKGROUND: Two lineages of Zika virus (ZIKV) have been classified according to the phylogenetic analysis: African and Asian lineages. It is unclear whether differences exist between the two strains in host cell permissiveness, this information is important for understanding viral pathogenesis and designing anti-viral strategies. METHODS: In the present study, we comparatively studied the permissive spectrum of human cells for both the African (MR766) and Asian strains (PRVABC59) using an RNA in situ hybridization (RISH) to visualize RNA replication, an immunofluorescence technology, and a western blot assay to determine viral protein production, and a real-time RT-PCR to examine viral RNA multiplication level...
March 31, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28346792/effects-of-ifn-%C3%AE-on-the-cell-growth-and-the-expression-of-adam33-gene-in-human-embryonic-lung-mrc-5-fibroblasts-in-vitro
#8
Wenjing Li, Rongrong Liang, Huarong Huang, Baojing Wu, Yingqiang Zhong
OBJECTIVE: To investigate the effects of interferon-γ (IFN-γ) on the proliferation and viability of human embryonic lung Mrc-5 fibroblasts in vitro and the expression of the A Disintegrin and Metalloprotease 33 (ADAM33) gene and to explore the mechanism of airway remodeling. METHODS: Mrc-5 fibroblasts were sensitized with Dermatophagoides farinae 1 (Derf1) in vitro to mimic in vivo conditions observed in bronchial asthma. An inverted fluorescence microscope was used to observe changes in cell morphology before and after treatment...
March 27, 2017: Journal of Asthma: Official Journal of the Association for the Care of Asthma
https://www.readbyqxmd.com/read/28340770/a-novel-polyaniline-polypyrrole-graphene-oxide-fiber-for-the-determination-of-volatile-organic-compounds-in-headspace-gas-of-lung-cell-lines
#9
JingHong Li, Hui Xu
Exploration of volatile organic compounds (VOCs) generated by lung cell lines is a powerful and non-invasive tool for the detection of potential volatile biomarkers of lung cancer. In this study, a simple and sensitive solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) method was developed for the determination of VOCs in the headspace gas of lung cell lines. For the purpose of preconcentration, a novel polyaniline/polypyrrole/graphene oxide (PANI/PPy/GO) coating was prepared on the surface of stainless steel fiber via in-situ electrochemical deposition for the first time...
May 15, 2017: Talanta
https://www.readbyqxmd.com/read/28297588/oncostatin-m-preconditioned-mesenchymal-stem-cells-alleviate-bleomycin-induced-pulmonary-fibrosis-through-paracrine-effects-of-the-hepatocyte-growth-factor
#10
Ying-Wei Lan, Si-Min Theng, Tsung-Teng Huang, Kong-Bung Choo, Chuan-Mu Chen, Han-Pin Kuo, Kowit-Yu Chong
Mesenchymal stem cells (MSCs) are widely considered for treatment of pulmonary fibrosis based on the anti-inflammatory, antifibrotic, antiapoptotic, and regenerative properties of the cells. Recently, elevated levels of oncostatin M (OSM) have been reported in the bronchoalveolar lavage fluid of a pulmonary fibrosis animal model and in patients. In this work, we aimed to prolong engrafted MSC survival and to enhance the effectiveness of pulmonary fibrosis transplantation therapy by using OSM-preconditioned MSCs...
March 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28290674/copper-i-phosphine-polypyridyl-complexes-synthesis-characterization-dna-hsa-binding-study-and-antiproliferative-activity
#11
Wilmer Villarreal, Legna Colina-Vegas, Gonzalo Visbal, Oscar Corona, Rodrigo S Corrêa, Javier Ellena, Marcia Regina Cominetti, Alzir Azevedo Batista, Maribel Navarro
A series of copper(I)-phosphine polypyridyl complexes have been investigated as potential antitumor agents. The complexes [Cu(PPh3)2dpq]NO3 (2), [Cu(PPh3)2dppz]NO3 (3), [Cu(PPh3)2dppa]NO3 (4), and [Cu(PPh3)2dppme]NO3 (5) were synthesized by the reaction of [Cu(PPh3)2NO3] with the respective planar ligand under mild conditions. These copper complexes were fully characterized by elemental analysis, molar conductivity, FAB-MS, and NMR, UV-vis, and IR spectroscopies. Interactions between these copper(I)-phosphine polypyridyl complexes and DNA have been investigated using various spectroscopic techniques and analytical methods, such as UV-vis titrations, thermal denaturation, circular dichroism, viscosity measurements, gel electrophoresis, and competitive fluorescent intercalator displacement assays...
April 3, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28289860/the-pd-l1-pd-1-pathway-promotes-dysfunction-but-not-exhaustion-in-tumor-responding-t-cells-from-pleural-effusions-in-lung-cancer-patients
#12
Heriberto Prado-Garcia, Susana Romero-Garcia, Alejandra Puerto-Aquino, Uriel Rumbo-Nava
Malignant pleural effusions are frequent in patients with advanced stages of lung cancer and are commonly infiltrated by lymphocytes and tumor cells. CD8+ T cells from these effusions have reduced effector functions. The programmed death receptor 1(PD-1)/programmed death ligand 1 (PD-L1) pathway is involved in T-cell exhaustion, and it might be responsible for T-cell dysfunction in lung cancer patients. Here, we show that PD-L1 is expressed on tumor cell samples from malignant effusions, on lung cancer cell lines, and, interestingly, on MRC-5 lung fibroblasts...
March 13, 2017: Cancer Immunology, Immunotherapy: CII
https://www.readbyqxmd.com/read/28282127/revisiting-previously-investigated-plants-a-molecular-networking-based-study-of-geissospermum-laeve
#13
Alexander E Fox Ramos, Charlotte Alcover, Laurent Evanno, Alexandre Maciuk, Marc Litaudon, Christophe Duplais, Guillaume Bernadat, Jean-François Gallard, Jean-Christophe Jullian, Elisabeth Mouray, Philippe Grellier, Philippe M Loiseau, Sébastien Pomel, Erwan Poupon, Pierre Champy, Mehdi A Beniddir
Three new monoterpene indole alkaloids (1-3) have been isolated from the bark of Geissospermum laeve, together with the known alkaloids (-)-leuconolam (4), geissolosimine (5), and geissospermine (6). The structures of 1-3 were elucidated by analysis of their HRMS and NMR spectroscopic data. The absolute configuration of geissolaevine (1) was deduced from the comparison of experimental and theoretically calculated ECD spectra. The isolation workflow was guided by a molecular networking-based dereplication strategy using an in-house database of monoterpene indole alkaloids...
March 10, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28258769/cytotoxicity-and-genotoxicity-of-a-low-shrinkage-monomer-and-monoacylphosphine-oxide-photoinitiator-comparative-analyses-of-individual-toxicity-and-combination-effects-in-mixtures
#14
Dragica Manojlovic, Miroslav D Dramićanin, Vesna Miletic, Dragana Mitić-Ćulafić, Bojana Jovanović, Biljana Nikolić
OBJECTIVE: To compare cytotoxicity and genotoxicity of novel urethane-based monomer FIT-852 and monoacylphosphine oxide photoinitiator (Lucirin TPO) with conventional Bisphenol A-glycidyl-methacrylate (BisGMA) and triethylene glycol dimethacrylate (TEGDMA) monomers and camphorquinone (CQ)/amine photoinitiator system, respectively. Moreover, we quantified and analyzed the combinatorial effects of individual substances in resin-based mixtures concerning the nature of the combinatorial effects...
February 28, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28243638/interstrand-dna-covalent-binding-of-two-dinuclear-ru-ii-complexes-influence-of-the-extra-ring-of-the-bridging-ligand-on-the-dna-interaction-and-cytotoxic-activity
#15
Héctor J Lozano, Natalia Busto, Gustavo Espino, Arancha Carbayo, José M Leal, James A Platts, Begoña García
In this work, we report experimental and computational evidence for the intercalation into the DNA base-pairs of the free quinones quinizarin (Q) and naphthazarin (N) and the interstrand covalent binding of their p-cymene di-ruthenium(ii) complexes (Cl2Ru2X, with X = N, Q bridging ligands). The intercalation extent for the N complex was larger than that for Q, which is in good agreement with the higher relative contour length and melting temperature for the same CX/CDNA ratio and with the computational mean stacking distances between the ligand and the nearest base-pair (3...
March 14, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28223069/evaluation-of-antibacterial-potential-and-toxicity-of-plant-volatile-compounds-using-new-broth-microdilution-volatilization-method-and-modified-mtt-assay
#16
Marketa Houdkova, Johana Rondevaldova, Ivo Doskocil, Ladislav Kokoska
With aim to develop effective proof-of-concept approach which can be used in a development of new preparations for the inhalation therapy, we designed a new screening method for simple and rapid simultaneous determination of antibacterial potential of plant volatiles in the liquid and the vapour phase at different concentrations. In addition, EVA (ethylene vinyl acetate) capmat™ as vapour barrier cover was used as reliable modification of thiazolyl blue tetrazolium bromide (MTT) assay for cytotoxicity testing of volatiles on microtiter plates...
April 2017: Fitoterapia
https://www.readbyqxmd.com/read/28203673/amphipathic-guanidine-embedded-glyoxamide-based-peptidomimetics-as-novel-antibacterial-agents-and-biofilm-disruptors
#17
Shashidhar Nizalapur, Onder Kimyon, Eugene Yee, Kitty Ho, Thomas Berry, Mike Manefield, Charles G Cranfield, Mark Willcox, David StC Black, Naresh Kumar
Antimicrobial resistance in bacteria is becoming increasingly prevalent, posing a critical challenge to global health. Bacterial biofilm formation is a common resistance mechanism that reduces the effectiveness of antibiotics. Thus, the development of compounds that can disrupt bacterial biofilms is a potential strategy to combat antimicrobial resistance. We report herein the synthesis of amphipathic guanidine-embedded glyoxamide-based peptidomimetics via ring-opening reactions of N-naphthoylisatins with amines and amino acids...
February 16, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28192961/raman-and-infrared-spectroscopy-distinguish-replicative-senescent-from-proliferating-primary-human-fibroblast-cells-by-detecting-spectral-differences-mainly-due-to-biomolecular-alterations
#18
Katharina Eberhardt, Claudia Beleites, Shiva Marthandan, Christian Matthäus, Stephan Diekmann, Juergen Popp
Cellular senescence is a terminal cell cycle arrested state, assumed to be involved in tumor suppression. We studied four human fibroblast cell strains (BJ, MRC 5, IMR 90 and WI 38) from proliferation into senescence. Cells were investigated by label-free vibrational Raman and infrared spectroscopy, following their transition into replicative senescence. During the transition into senescence, we observed rather similar biomolecular abundances in all four cell strains and between proliferating and senescent cells; however, in the four aging cell strains we found common molecular differences dominated by protein and lipid modifications...
January 27, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28173977/identification-of-a-candidate-standard-strain-of-severe-fever-with-thrombocytopenia-syndrome-virus-for-vaccine-quality-control-in-china-using-a-cross-neutralization-assay
#19
Zheng Jia, Xiaohong Wu, Ling Wang, Xiuling Li, Xinxian Dai, Mifang Liang, Shouchun Cao, Yan Kong, Jingjing Liu, Yuhua Li, Junzhi Wang
Severe fever with thrombocytopenia syndrome (SFTS) is caused by a phlebovirus of the Bunyaviridae family, which is designated as SFTS virus (SFTSV). To our knowledge, no efficient SFTSV vaccine exists. Here, we report the identification of a standard virus strain for the eight major SFTSV strains circulating in China for use in evaluating the SFTSV vaccine. Rabbits were immunized with the SFTSV strains and the cross-neutralization capacities of SFTSV anti-sera were determined in microculture cytopathic effect (CPE)-inhibition assays...
March 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28161627/temperature-and-ph-sensors-based-on-graphenic-materials
#20
P Salvo, N Calisi, B Melai, B Cortigiani, M Mannini, A Caneschi, G Lorenzetti, C Paoletti, T Lomonaco, A Paolicchi, I Scataglini, V Dini, M Romanelli, R Fuoco, F Di Francesco
Point-of-care applications and patients' real-time monitoring outside a clinical setting would require disposable and durable sensors to provide better therapies and quality of life for patients. This paper describes the fabrication and performances of a temperature and a pH sensor on a biocompatible and wearable board for healthcare applications. The temperature sensor was based on a reduced graphene oxide (rGO) layer that changed its electrical resistivity with the temperature. When tested in a human serum sample between 25 and 43°C, the sensor had a sensitivity of 110±10Ω/°C and an error of 0...
May 15, 2017: Biosensors & Bioelectronics
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