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https://www.readbyqxmd.com/read/27830183/human-cytomegalovirus-infection-enhances-nk-cell-activity-in-vitro
#1
Astrid Tschan-Plessl, Martin Stern, Laurent Schmied, Christelle Retière, Hans H Hirsch, Christian Garzoni, Christian van Delden, Katia Boggian, Nicolas J Mueller, Christoph Berger, Jean Villard, Oriol Manuel, Pascal Meylan, Grzegorz Terszowski
BACKGROUND: Occurring frequently after solid organ and hematopoietic stem cell transplantation, cytomegalovirus (CMV) replication remains a relevant cause of mortality and morbidity in affected patients. Despite these adverse effects, an increased alloreactivity of natural killer (NK) cells after CMV infection has been assumed, but the underlying physiopathological mechanisms have remained elusive. METHODS: We used serial analyses of NK cells before and after CMV infection in kidney transplant recipients as an in vivo model for CMV primary infection to explore the imprint of CMV infection using every patient as their own control: We analyzed NK cell phenotype and function in 47 CMV seronegative recipients of CMV seropositive kidney grafts, who developed CMV primary infection posttransplant...
July 2016: Transplantation Direct
https://www.readbyqxmd.com/read/27812019/measurement-of-in-plane-elasticity-of-live-cell-layers-using-a-pressure-sensor-embedded-microfluidic-device
#2
Chien-Han Lin, Chien-Kai Wang, Yu-An Chen, Chien-Chung Peng, Wei-Hao Liao, Yi-Chung Tung
In various physiological activities, cells experience stresses along their in-plane direction when facing substrate deformation. Capability of continuous monitoring elasticity of live cell layers during a period is highly desired to investigate cell property variation during various transformations under normal or disease states. This paper reports time-lapsed measurement of live cell layer in-plane elasticity using a pressure sensor embedded microfluidic device. The sensor converts pressure-induced deformation of a flexible membrane to electrical signals...
November 4, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27811159/in-vitro-cytotoxicity-and-genotoxicity-of-composite-mixtures-of-natural-rubber-and-leather-residues-used-for-textile-applications
#3
Dalita Gsm Cavalcante, Andressa S Gomes, Elton Ap Dos Reis, Caroline S Danna, Leandra E Kerche-Silva, Eidi Yoshihara, Aldo E Job
A novel composite material has been developed from natural rubber and leather waste, and a corresponding patent has been filed. This new material may be incorporated into textile and footwear products. However, as leather waste contains chromium, the biocompatibility of this new material and its safety for use in humans must be investigated. The aim of the present study was to investigate the presence of chromium in this new material, determine the amount of each form of chromium present (trivalent or hexavalent), and evaluate the potential cytotoxic and genotoxic effects of the novel composite in two cell lines...
November 3, 2016: Toxicology and Industrial Health
https://www.readbyqxmd.com/read/27806706/rice-callus-suspension-culture-inhibits-growth-of-cell-lines-of-multiple-cancer-types-and-induces-apoptosis-in-lung-cancer-cell-line
#4
Nafeesa Rahman, Surendar Reddy Dhadi, Aparna Deshpande, Wusirika Ramakrishna
BACKGROUND: Cancer is one of the leading cause of mortality. Even though efficient drugs are being produced to treat cancer, conventional medicines are costly and have adverse effects. As a result, alternative treatments are being tried due to their low cost and little or no adverse effects. Our previous study identified one such alternative in rice callus suspension culture (RCSC) which was more efficient than Taxol® and Etoposide, in reducing the viability of human colon and renal cancer cells in culture with minimal or no effect on a normal cell line...
November 2, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27805201/new-ru-ii-pincer-complexes-synthesis-characterization-and-biological-evaluation-for-photodynamic-therapy
#5
Leila Tabrizi, Hossein Chiniforoshan
Three new ruthenium(ii) complexes of NCN pincer and phenylcyanamide derivative ligands of the formula [Ru(L)(Ph2phen)(3,5-(NO2)2pcyd)], 1, [Ru(L)(Me2phen)(3,5-(NO2)2pcyd)], 2, and [Ru(L)(Cl2phen)(3,5-(NO2)2pcyd)], 3 (HL: 5-methoxy-1,3-bis(1-methyl-1H-benzo[d]imidazol-2-yl)benzene, 3,5-(NO2)2pcyd: 3,5-(NO2)2pcyd, Ph2phen: 4,7-diphenyl-1,10-phenanthroline, Me2phen: 4,7-dimethyl-1,10-phenanthroline, Cl2phen: 4,7-dichloro-1,10-phenanthroline) have been synthesized and studied as potential photosensitizers (PSs) in photodynamic therapy (PDT)...
November 2, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/27801583/synthesis-and-antineoplastic-evaluation-of-novel-unsymmetrical-1-3-4-oxadiazoles
#6
Valentina Nieddu, Giansalvo Pinna, Irene Marchesi, Luca Sanna, Battistina Asproni, Gérard Aimè Pinna, Luigi Marco Bagella, Gabriele Murineddu
A series of novel unsymmetrical 2,5-disubstituted 1,3,4-oxadiazoles was synthetized and evaluated for their cytotoxic activity against different cancer cell lines of various origins as in vitro tumor models. Four of 12 new compounds (2b, 2h, 2j and 2l) showed growth inhibitory activity using the XTT dye assay. The most active agent, 2-(2-methyl-1,4-dihydroindeno[1,2-b]pyrrol-3-yl)-5-phenyl-1,3,4-oxadiazole, 2j, was chosen for further studies. It showed high potency against a panel of cultured human cancer cells with IC50 ranging from 0...
November 1, 2016: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/27798371/oncostatin-m-preconditioned-mesenchymal-stem-cells-alleviate-bleomycin-induced-pulmonary-fibrosis-through-paracrine-effects-of-the-hepatocyte-growth-factor
#7
Ying-Wei Lan, Si-Min Theng, Tsung-Teng Huang, Kong-Bung Choo, Chuan-Mu Chen, Han-Pin Kuo, Kowit-Yu Chong
: : Mesenchymal stem cells (MSCs) are widely considered for treatment of pulmonary fibrosis based on the anti-inflammatory, antifibrotic, antiapoptotic, and regenerative properties of the cells. Recently, elevated levels of oncostatin M (OSM) have been reported in the bronchoalveolar lavage fluid of a pulmonary fibrosis animal model and in patients. In this work, we aimed to prolong engrafted MSC survival and to enhance the effectiveness of pulmonary fibrosis transplantation therapy by using OSM-preconditioned MSCs...
October 18, 2016: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/27790250/cytotoxic-activity-of-androctonus-australis-hector-venom-and-its-toxic-fractions-on-human-lung-cancer-cell-line
#8
Louisa Béchohra, Fatima Laraba-Djebari, Djelila Hammoudi-Triki
BACKGROUND: Several studies have showed that animal venoms are a source of bioactive compounds that may inhibit the growth of cancer cells, which makes them useful agents for therapeutic applications. Recently, it was established that venom toxins from scorpions induced cytotoxic, antiproliferative and apoptogenic effects on cancer cells. Therefore, the present study aims to investigate the cytotoxic activity of Androctonus australis hector (Aah) scorpion venom and its toxic fractions (FtoxG-50 and F3) on NCI-H358 human lung cancer cells...
2016: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/27786573/effect-and-mechanism-of-calpains-on-pediatric-lobar-pneumonia
#9
Genquan Yin, Qiang Zeng, Haijin Zhao, Peiqiong Wu, Shaoxi Cai, Li Deng, Wenhui Jiang
BACKGROUND: Lobar pneumonia, one of the community-acquired pneumonia (CAP), is a common pediatric low respiratory tract infection. Calpains are Ca(2+)-activated cysteine proteases whose activation mechanism is elusive. The present study was undertaken to detect the role and mechanism of calpains in pediatric lobar pneumonia. MATERIAL AND METHODS: The human acute lung infection model (ALIM) was constructed and infected by Streptococcus. Enzyme-linked immunosorbent assay (ELISA) was used to measure interleukin (IL)-6, IL-8 and tumor necrosis factor (TNF)-α...
October 27, 2016: Bioengineered
https://www.readbyqxmd.com/read/27764812/microrna-7-inhibits-cell-proliferation-migration-and-invasion-in-human-non-small-cell-lung-cancer-cells-by-targeting-fak-through-erk-mapk-signaling-pathway
#10
Qi Cao, Zheng-Dao Mao, Yu-Jia Shi, Yi Chen, Yun Sun, Qian Zhang, Lei Song, Li-Ping Peng
OBJECTIVE: To investigate the effects of microRNA-7 (miR-7) on the proliferation, migration and invasion of non-small cell lung cancer NSCLC) cells by targeting FAK through ERK/MAPK signaling pathway. METHODS: NSCLC tissues and adjacent normal tissues were obtained from 160 NSCLC patients after operation. NSCLC cell lines (A549, H1299 and H1355) and a normal human fetal lung fibroblast cell line (MRC-5) were obtained. NSCLC cells were assigned into miR-7 inhibitors, miR-7 mimics, blank, miR-7 mimics control, miR-7 inhibitors control, FAK siRNA and miR-7 inhibitors + FAK siRNA groups...
October 15, 2016: Oncotarget
https://www.readbyqxmd.com/read/27757268/in-vitro-antileishmanial-and-antimalarial-activity-of-selected-plants-of-nepal
#11
Bishnu Joshi, Sarah Hendrickx, Lila Bahadur Magar, Niranjan Parajuli, Pierre Dorny, Louis Maes
BACKGROUND: Nepal is very rich in biodiversity, and no extensive effort has yet been carried out to screen plants that are used by traditional healers against parasitic diseases. The aim of this study was to evaluate the in vitro antileishmanial and antimalarial activity of crude methanolic or ethanolic extracts of 29 plant species that are currently used by local people of Nepal for treating different ailments. METHODS: Crude extracts of leaves, twigs, aerial parts, and/or roots of the selected plants were evaluated for in vitro inhibitory activity against intracellular amastigotes of Leishmania infantum and against erythrocytic stages of Plasmodium falciparum...
September 2016: Journal of Intercultural Ethnopharmacology
https://www.readbyqxmd.com/read/27748782/assessment-of-the-nematocidal-activity-of-metallocenyl-analogues-of-monepantel
#12
Jeannine Hess, Malay Patra, Abdul Jabbar, Vanessa Pierroz, Sandro Konatschnig, Bernhard Spingler, Stefano Ferrari, Robin B Gasser, Gilles Gasser
In this study, we present the design, synthesis, characterization and biological evaluation of structurally new ferrocenyl and ruthenocenyl derivatives of the organic anthelmintic monepantel (Zolvix(®)). All seven metallocenyl derivatives prepared (4a/b, 5a/b, 6a/b and 7) were isolated as racemates and characterized by (1)H, (13)C and (19)F NMR spectroscopies, mass spectrometry, IR spectroscopy and elemental microanalysis. The molecular structures of four compounds (4a/b, 6a and 7) were further confirmed by X-ray crystallography...
October 17, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/27734223/basic-fibroblast-growth-factor-activates-%C3%AE-catenin-rhoa-signaling-in-pulmonary-fibroblasts-with-chronic-obstructive-pulmonary-disease-in-rats
#13
Zhengxing Ge, Bo Li, Xun Zhou, Yi Yang, Jun Zhang
Chronic obstructive pulmonary disease (COPD) is featured by aberrant extracellular matrix (ECM) deposition. Trigger of the β-catenin/RhoA pathway has been involved in aberrant ECM deposition in several diseases. We investigated WNT signaling activation in primary pulmonary fibroblasts of rats with and without COPD and the function of WNT signaling in pulmonary fibroblast. We evaluated the expression of WNT signaling and the role of β-catenin, using MRC-5 fibroblasts and primary lung fibroblasts of rats with and without COPD...
October 13, 2016: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/27725857/protective-effect-of-bone-marrow-derived-mesenchymal-stem-cells-in-lipopolysaccharide-induced-acute-lung-injury-mediated-by-claudin-4-in-a-rat-model
#14
Yueliang Zheng, Wenwei Cai, Shengang Zhou, Liming Xu, Chengxing Jiang
Our study aims to investigate the effects of bone marrow derived mesenchymal stem cells (BM-MSCs) in lipopolysaccharide (LPS)-induced acute lung injury (ALI) as well as the underlying mechanism. In our study, Wistar rats were randomly divided into four groups: control group; ALI group; ALI+MSCs group and ALI+MSCs claudin-4 siRNA group. MRC-5 and BEAS-2B cell lines were used for in vitro assay. Flow cytometry, western blot, hematoxylin and eosin (H&E) staining, CCK-8 assay, enzyme-linked immunosorbent assay (ELISA) were involved to measure the pathological changes in lung tissues...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27709812/a-novel-filtration-system-for-point-of-care-washing-of-cellular-therapy-products
#15
Rui Tostoes, John R Dodgson, Ben Weil, Spyridon Gerontas, Chris Mason, Farlan Veraitch
The cell therapy industry would greatly benefit from a simple point of care solution to remove dimethylsulphoxide (DMSO) from small-volume thawed cell suspensions before injection. A novel dead-end filtration device has been designed and validated, which takes advantage of the higher density of thawed cell suspensions to remove the DMSO and protein impurities from the cell suspension without fouling the filter membrane. The filter was designed to avoid fluid circuits and minimize the surface area that is contacted by the cell suspension, thus reducing cell losses by design...
October 6, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27707992/in-vitro-time-to-kill-assay-to-assess-the-cidal-activity-dynamics-of-current-reference-drugs-against-leishmania-donovani-and-leishmania-infantum
#16
L Maes, J Beyers, A Mondelaers, M Van den Kerkhof, E Eberhardt, G Caljon, S Hendrickx
OBJECTIVES: Despite a continued search for novel antileishmanial drugs, treatment options remain restricted to a few standard drugs, e.g. antimonials, miltefosine, amphotericin B and paromomycin. Although these drugs have now been used for several decades, their mechanism of action still remains partly hypothetical and their dynamics of cidal action and time-to-kill are still poorly documented. METHODS: An in vitro time-to-kill assay on intracellular amastigotes of the laboratory reference strains Leishmania donovani (MHOM/ET/67/L82) and Leishmania infantum [MHOM/MA(BE)/67/ITMAP263] evaluated the cidal action dynamics of the listed reference drugs at three different concentrations: at IC50, 2 × IC50 and the near cytotoxic dose level (CC90: determined on MRC-5 cells)...
October 5, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27694024/toxicity-assessment-of-carbon-black-waste-a-by-product-from-oil-refineries
#17
Xu Zhen, Wei Cheng Ng, Fendy, Yen Wah Tong, Yanjun Dai, Koon Gee Neoh, Chi-Hwa Wang
In Singapore, approximately 30t/day of carbon-based solid waste are produced from petrochemical processes. This carbon black waste has been shown to possess physical properties that are characteristic of a good adsorbent such as high external surface area. Therefore, there is a growing interest to reutilize and process this carbon black waste into secondary materials such as adsorbents. However, the carbon black waste obtained from petrochemical industries may contain heavy metals that are hazardous to human health and the environment, hence restricting its full potential for re-utilization...
September 20, 2016: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/27693770/in-vitro-antiprotozoal-activity-and-cytotoxicity-of-extracts-and-isolated-constituents-from-greenwayodendron-suaveolens
#18
D Musuyu Muganza, B Fruth, J Lami Nzunzu, E Tuenter, K Foubert, P Cos, L Maes, R Cimanga Kanyanga, V Exarchou, S Apers, L Pieters
ETHNOPHARMACOLOGICAL RELEVANCE: The Nkundo people (Nkundo area of Bolongo, Mai-Ndombe district, Bandundu Province, DR Congo) use various plant parts of the tree Greenwayodendron suaveolens (Engl. & Diels) Verdc. (syn. Polyalthia suaveolens Engl. & Diels) (Annonaceae) against malaria, but its antiprotozoal constituents are not known. MATERIALS AND METHODS: The crude 80% ethanol extract from the fruits, leaves, root bark and stem bark and 16 fractions were assessed in vitro for their antiprotozoal activity against Trypanosoma brucei brucei, T...
September 29, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27693418/tumour-necrosis-factor-%C3%AE-induces-heparan-sulfate-6-o-endosulfatase-1-sulf-1-expression-in-fibroblasts
#19
Anne-Sophie Sikora, Charles Hellec, Mathieu Carpentier, Pierre Martinez, Maxime Delos, Agnès Denys, Fabrice Allain
Heparan sulfate (HS) 6-O-endosulfatases (Sulfs) have emerged recently as critical regulators of many physiological and pathological processes. By removing 6-O-sulfates from specific HS sequences, they modulate the activities of a variety of growth factors and morphogens, including fibroblast growth factor (FGF)-1. However, little is known about the functions of Sulfs in inflammation. Tumour-necrosis factor (TNF)-α plays an important role in regulating the behaviour of fibroblasts. In this study, we examined the effect of this inflammatory cytokine on the expression of Sulfs in human MRC-5 fibroblasts...
September 28, 2016: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/27690085/enhanced-replication-of-hepatitis-e-virus-strain-47832c-in-an-a549-derived-subclonal-cell-line
#20
Mathias Schemmerer, Silke Apelt, Eva Trojnar, Rainer G Ulrich, Jürgen J Wenzel, Reimar Johne
Hepatitis E virus (HEV) is a human pathogen with increasing importance. The lack of efficient cell culture systems hampers systematic studies on its replication cycle, virus neutralization and inactivation. Here, several cell lines were inoculated with the HEV genotype 3c strain 47832c, previously isolated from a chronically infected transplant patient. At 14 days after inoculation the highest HEV genome copy numbers were found in A549 cells, followed by PLC/PRF/5 cells, whereas HepG2/C3A, Huh-7 Lunet BLR and MRC-5 cells only weakly supported virus replication...
2016: Viruses
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