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Ana Galán-Cobo, Elena Arellano-Orden, Rocío Sánchez Silva, José Luis López-Campos, César Gutiérrez Rivera, Lourdes Gómez Izquierdo, Nela Suárez-Luna, María Molina-Molina, José A Rodríguez Portal, Miriam Echevarría
Activation of the epithelial-mesenchymal transition process (EMT) by which alveolar cells in human lung tissue undergo differentiation giving rise to a mesenchymal phenotype (fibroblast/miofibroblasts) has been well recognized as a key element in the origin of idiopathic pulmonary fibrosis (IPF). Here we analyzed expression of AQP1 in lung biopsies of patients diagnosed with IPF, and compared it to biopsies derived from patients with diverse lung pneumonies, such as hypersensitivity pneumonitis, sarcoidosis or normal lungs...
2018: Frontiers in Molecular Biosciences
JingHong Li, Nan Chen, YiPing Tian, Hui Xu
The exploration of volatile organic compounds (VOCs) produced by cell lines may be a powerful and non-invasive tool for the study of the health risk of human exposure to atmospheric particulate matter (PM). In this work, we developed a sensitive solid phase microextraction-gas chromatography-mass spectrometry method (SPME-GC-MS) to analyze VOCs in breathed gas of PM2.5-induced human embryonic fibroblast cell line (MRC-5). A novel graphene oxide/polyaniline/polydopamine (GO/PANI/PDA) coating was prepared on a stainless steel wire via electrochemical deposition and self-polymerization for the first time...
August 1, 2018: Talanta
Mariagrazia di Luca, Orazio Vittorio, Giuseppe Cirillo, Manuela Curcio, Magdalena Czuban, Florida Voli, Annafranca Farfalla, Silke Hampel, Fiore Pasquale Nicoletta, Francesca Iemma
A free radical polymerization method was adopted for the fabrication of hybrid hydrogel films based on acrylamide and polyethylene glycol dimethacrylate as plasticizing and crosslinking agents, respectively, to be employed as smart skin bandages. Electro-sensitivity, biocompatibility and proteolytic properties were conferred to the final polymer networks by introducing graphene oxide (0.5% w/w), gelatin or trypsin (10% w/w) in the polymerization feed. The physical chemical and mechanical characterization of hybrid materials was performed by means of determination of protein content, Raman spectroscopy, thermogravimetric analysis and measurement of tensile strength...
May 11, 2018: International Journal of Pharmaceutics
Nenad L Ignjatović, Katarina M Penov-Gaši, Jovana J Ajduković, Vesna V Kojić, Smilja B Marković, Dragan P Uskoković
An androstane (17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (derivative A)) cancer inhibitor was successfully captured in a carrier made of nano-sized hydroxyapatite (HAp) coated with chitosan-PLGA polymer blends (Ch-PLGA). In our previous studies, we demonstrated that it was convenient to use spherical HAp/Ch-PLGA carriers as vehicles to target the lungs following intravenous administration. In this study, we used emulsification and subsequent freeze-drying to load the spherical HAp/Ch-PLGA carriers with varying contents of the derivative A, in order to examine the selective toxicity towards cancerous/healthy lung cells...
August 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
Diego Alberti, Annamaria Deagostino, Antonio Toppino, Nicoletta Protti, Silva Bortolussi, Saverio Altieri, Silvio Aime, Simonetta Geninatti Crich
The aim of this study is to develop an innovative imaging guided approach based on Boron Neutron Capture Therapy, for the treatment of mesothelioma, assisted by the quantification of the in vivo boron distribution by MRI. The herein reported results demonstrate that overexpressed Low Density Lipoproteins receptors can be successfully exploited to deliver to mesothelioma cells a therapeutic dose of boron (26 μg/g), significantly higher than in the surrounding tissue (3.5 μg/g). Boron and Gd cells uptake was assessed by ICP-MS and MRI on two mesothelioma (ZL34, AE17) and two healthy (MRC-5 and NMuMg) cell lines...
May 3, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
Azren Aida Asmawi, Norazlinaliza Salim, Cheng Loong Ngan, Haslina Ahmad, Emilia Abdulmalek, Mas Jaffri Masarudin, Mohd Basyaruddin Abdul Rahman
Docetaxel has demonstrated extraordinary anticancer effects on lung cancer. However, lack of optimal bioavailability due to poor solubility and high toxicity at its therapeutic dose has hampered the clinical use of this anticancer drug. Development of nanoemulsion formulation along with biocompatible excipients aimed for pulmonary delivery is a potential strategy to deliver this poorly aqueous soluble drug with improved bioavailability and biocompatibility. In this work, screening and selection of pharmaceutically acceptable excipients at their minimal optimal concentration have been conducted...
April 24, 2018: Drug Delivery and Translational Research
Daniela V Šojić Merkulov, Vesna N Despotović, Nemanja D Banić, Sanja J Armaković, Nina L Finčur, Marina J Lazarević, Dragana D Četojević-Simin, Dejan Z Orčić, Marija B Radoičić, Zoran V Šaponjić, Mirjana I Čomor, Biljana F Abramović
A comprehensive study of the removal of selected biologically active compounds (pharmaceuticals and pesticides) from different water types was conducted using bare TiO2 nanoparticles and TiO2 /polyaniline (TP-50, TP-100, and TP-150) nanocomposite powders. In order to investigate how molecular structure of the substrate influences the rate of its removal, we compared degradation efficiency of the initial substrates and degree of mineralization for the active components of pharmaceuticals (propranolol, and amitriptyline) and pesticides (sulcotrione, and clomazone) in double distilled (DDW) and environmental waters...
April 18, 2018: Environmental Pollution
Sara Vieira, Albina R Franco, Emanuel M Fernandes, Sara Amorim, Helena Ferreira, Ricardo A Pires, Rui L Reis, Albino Martins, Nuno M Neves
Fish sarcoplasmic proteins (FSP) constitute around 25-30% of the total fish muscle protein. As the FSP are water soluble, FSP were isolated from fresh cod (Gadus morhua) by centrifugation. By SDS-PAGE, it was possible to determine the composition of FSP extracts (FSP-E). The FSP-E undergo denaturation at 44.12 ± 2.34° C, as characterized by differential scanning calorimetry thermograms (DSC). The secondary structure of FSP-E is mainly composed by α-helix structure, as determined by circular dichroism...
April 5, 2018: Colloids and Surfaces. B, Biointerfaces
Xiaoping Luo, Haihui Zhang, Yuqing Duan, Guangying Chen
Natural antioxidants play a critical role in the promotion of good health for its prevention of oxidative stress. The main purpose of this study is to investigate the protective effects of radish leaves extract on the oxidative damage in human fetal lung fibroblast (MRC-5) cells. F2, a fraction of radish leaves extracts, which was fractionated by different polarity solvents and AB-8 macroporous resins column shows the best free radical scavenging ability, the highest total polyphenol contents (TPC), and the most potent protective effects on H2 O2 -induced oxidative damage in MRC-5 cells...
April 16, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Hussein H K Abbas, Kheloud M H Alhamoudi, Mark D Evans, George D D Jones, Steven S Foster
BACKGROUND: Targeted therapies are based on exploiting cancer-cell-specific genetic features or phenotypic traits to selectively kill cancer cells while leaving normal cells unaffected. Oxidative stress is a cancer hallmark phenotype. Given that free nucleotide pools are particularly vulnerable to oxidation, the nucleotide pool sanitising enzyme, MTH1, is potentially conditionally essential in cancer cells. However, findings from previous MTH1 studies have been contradictory, meaning the relevance of MTH1 in cancer is still to be determined...
April 16, 2018: BMC Cancer
Zhen Liu, Jingyi Zhao, Xiao Liang, Xiaoxi Lv, Yong Li, Jing Qu, Yunbao Liu
Nine new compounds (1-6 and 16-18) and nine known compounds (7-15) were isolated from the Diaporthe pseudomangiferaea, an endophytic fungus obtained from the leaves of the toxic Chinese folk medicine Tylophora ouata. Their structures were elucidated by NMR spectroscopy and MS spectrometry analyses. The absolute configurations were established according to the specific rotation or electron circular dichroism method. Compounds 1, 4, 9, 11, 14 and 15 inhibited the TFG-β induced activation of human lung fibroblasts MRC-5 cells by 17...
April 13, 2018: Fitoterapia
Jelena Petronijević, Nenad Janković, Tatjana P Stanojković, Nenad Joksimović, Nađa Đ Grozdanić, Milan Vraneš, Aleksandar Tot, Zorica Bugarčić
In order to investigate new potential therapeutically active agents, we investigated the biological properties of two small libraries of quinoxalinones and 1,4-benzoxazin-2-ones. The results obtained showed that compounds 5, 9-11 have good cytotoxic activity against HeLa cells where the lowest IC50 value (10.46 ± 0.82 μM/mL) was measured for compound 10. Additionally, the most active compounds (5, 9-11) showed much better selectivity for MRC-5 cells (up to 17.4) compared to cisplatin. In vitro evaluation of the inhibition of the enzyme α-glucosidase showed that compounds 10 and 11 exert significant inhibition of the enzyme at 52...
April 14, 2018: Archiv der Pharmazie
Valeria R Martínez, María V Aguirre, Juan S Todaro, Oscar E Piro, Gustavo A Echeverría, Luciana G Naso, Evelina G Ferrer, Patricia A M Williams
A new losartan [2-butyl-5-chloro-3-[[4-[2-(2H-tetrazol-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol zinc(II) complex [Zn(Los)Cl], was synthesized and characterized. The crystal structure was determined by x-ray diffraction methods. When aqueous solutions of the ligand and the metal were mixed, the known and more soluble powder [Zn(Los)2 ].3H2 O (ZnLos) complex has been obtained. The interactions with phosphatases showed a concerted mechanism displayed by the Zn ions and ZnLos up to 500 μM concentration: a decrease of the acid phosphatase (AcP) associated with an increase in the alkaline phosphatase (ALP) activities...
April 12, 2018: Biological Trace Element Research
Minxiao Yi, Bo Liu, Yang Tang, Fang Li, Wan Qin, Xianglin Yuan
Radiation induced pulmonary fibrosis (RIPF) is one of the major side effects of radiotherapy for lung cancer. Previous studies have shown that endothelial cells and activated myofibroblasts play a key role in RIPF. However, the interaction between irradiated endothelial cells and activation of myofibroblasts has not been reported. The aim of the present study was to examine whether irradiated endothelial cells would affect the differentiation of fibroblasts into myofibroblasts in the process of RIPF. In the current study, we used a coculture system that allowed direct contact between human fetal lung fibroblasts (MRC-5) and irradiated human umbilical vein endothelial cells (HUVECs)...
2018: BioMed Research International
Sun Young Kyung, Dae Young Kim, Jin Young Yoon, Eun Suk Son, Yu Jin Kim, Jeong Woong Park, Sung Hwan Jeong
BACKGROUND: Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal disease with no effective treatment. The epithelial-mesenchymal transition (EMT) is a critical stage during the development of fibrosis. To assess the effect of sulforaphane (SFN) on the EMT and fibrosis using an in vitro transforming growth factor (TGF)-β1-induced model and an in vivo bleomycin (BLM)-induced model. METHODS: In vitro studies, cell viability, and cytotoxicity were measured using a Cell Counting Kit-8...
April 2, 2018: BMC Pharmacology & Toxicology
Karakaya Gulsah, Ercan Ayse, Oncu Selin, Aytemir Mutlu Dilsiz
In the present study, Mannich bases of kojic acid with the structure of 2-substituted-3-hydroxy-6-hyroxymethyl/chloromethyl/methyl/morpholinomethyl/piperidinylmethyl/pyrrolidinylmethyl-4H-pyran-4-one (compounds 1-23) were synthesized by the reaction of kojic acid/chlorokojic acid/allomaltol and substituted benzylpiperazine derivatives in presence of formaline. To obtain the cyclic amine (morpholine, piperidine and pyrrolidine) derivatives of the 6th-position of chlorokojic acid derivatives, nucleophilic substitutions were carried out...
April 2, 2018: Anti-cancer Agents in Medicinal Chemistry
Tiane C Finimundy, Gustavo Scola, Fernando J Scariot, Aldo J P Dillon, Sidnei Moura, Sérgio Echeverrigaray, João Pegas Henriques, Mariana Roesch-Ely
Cumulative evidence from research studies has shown that the shiitake culinary-medicinal mushroom, Lentinus edodes, is an excellent source of natural antitumor agents and is capable of inhibiting cancer cell growth. However, the cell signaling pathway that leads tumor cells to apoptosis is not well understood because many chemical compounds may be acting. This study investigated the chemopreventive effects of an L. edodes aqueous extract on human HEp-2 epithelial larynx carcinoma cells and normal human MRC-5 lung fibroblasts by identifying proliferative and apoptotic pathways...
2018: International Journal of Medicinal Mushrooms
Andrea R Nikolić, Ivana Z Kuzminac, Suzana S Jovanović-Šanta, Dimitar S Jakimov, Lidija D Aleksić, Marija N Sakač
Steroidal 16,17-seco-16,17a-dinitriles possessing 4-ene-3,6-dione (3), 6-methylene-4-en-3-one (5), (6E)-hydroxyimino-4-en-3β-ol (9) or (6E)-hydroxyimino-4-en-3-one (10) moiety were synthesized starting from 3β-acetoxy-16,17-secoandrost-4-ene-16,17a-dinitrile (1). Antiproliferative activity of the newly synthesized compounds, as well as previously synthesized 3-oxo-16,17-secoandrosta-1,4-diene-16,17a-dinitrile (VII), was tested in vitro. Compound 9 displayed submicromolar antiproliferative activity against human cervical carcinoma (HeLa) cells (IC50 0...
March 28, 2018: Steroids
Lizette Anayo, Anette Magnussen, Alexis Perry, Mark Wood, Alison Curnow
OBJECTIVES: Non-melanoma skin cancers are the most frequently occurring type of cancer worldwide. They can be effectively treated using topical dermatological photodynamic therapy (PDT) employing protoporphyrin IX (PpIX) as the active photosensitising agent as long as the disease remains superficial. Novel iron chelating agents are being investigated to enhance the effectiveness and extend the applications of this treatment modality, as limiting free iron increases the accumulation of PpIX available for light activation and thus cell kill...
March 31, 2018: Lasers in Surgery and Medicine
Claudia Martínez-García, Yolanda Medina-Flores, José Manuel de la Rosa-Vázquez, Eva Elda Soriano-Pérez, Juan Ramón Villalobos-Hernández, Eva Ramón-Gallegos
BACKGROUND: In this work, a drug product composed of an IgM antibody derived from a hybridoma subclone 4C1F6D5G7B8 was prepared and further labeled with PpIX to be used in cell lines A-549 and MRC-5. The aim of this work was to evaluate the potential theranostic activity of the obtained product together with photodynamic therapy (PDT). METHODS: The IgM antibody labeled with PpIX was used in different concentrations to perform theranostics with PDT in cell lines A-549 and MRC-5 in order to identify the specificity of IgM antibody in lung cancer cells by means of a LED-irradiation system set at 630 nm...
March 27, 2018: Photodiagnosis and Photodynamic Therapy
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