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https://www.readbyqxmd.com/read/29138118/8-cetylberberine-inhibits-growth-of-lung-cancer-in-vitro-and-in-vivo
#1
Yubo Xiao, Cheng Tian, Tao Huang, Bing Han, Meimei Wang, Hang Ma, Zhaoxing Li, Xiaoli Ye, Xuegang Li
AIMS: This study is aimed at detecting the anti-tumor efficacy of a new berberine (BBR) derivative 8-cetylberberine (HBBR), which has a significant improvement in hydrophobicity and pharmacological effects compared to BBR. MAIN METHODS: The human non-small lung cancer cell line A549 and normal human lung epithelial cells (MRC-5) were cultured to observe inhibition in vitro. Cell viability was analyzed via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay...
November 11, 2017: Life Sciences
https://www.readbyqxmd.com/read/29133038/new-metalo-therapeutics-of-nsaids-against-human-breast-cancer-cells
#2
Christina N Banti, Constantina Papatriantafyllopoulou, Anastasios J Tasiopoulos, Sotiris K Hadjikakou
The non steroidal anti-inflammatory drugs (NSAID's)-silver(I) metallodrugs of aspirin (aspH), salicylic acid (salH2), naproxen (napH) acid or p-hydrobenzoic acid (pHbzaH) and the mitochondriotropic triphenylarsine (tpAs) with the formulae [Ag(asp)(tpAs)3] (1), [Ag(salH)(tpAs)3] (2), [Ag(nap)(tpAs)3] (3) and {[Ag(pHbza)(tpAs)3]∙(dmf)} (4) and [Ag(tpAs)3(NO3)] (5) have been synthesized and characterized by m.p., FT-IR, UV-vis and (1)H NMR, spectroscopic techniques and X-ray crystallography. The in vitro cytotoxic activity of 1-5 against human breast adenocarcinoma cancer cells: MCF-7 (positive to estrogen receptors (ERs)) and MDA-MB-231 (negative to estrogen receptors (ERs)) was evaluated...
November 10, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29122364/photocatalytic-degradation-of-4-amino-6-chlorobenzene-1-3-disulfonamide-stable-hydrolysis-product-of-hydrochlorothiazide-detection-of-intermediates-and-their-toxicity
#3
Sanja J Armaković, Stevan Armaković, Dragana D Četojević-Simin, Filip Šibul, Biljana F Abramović
In this work we have investigated in details the process of degradation of the 4-amino-6-chlorobenzene-1,3-disulfonamide (ABSA), stable hydrolysis product of frequently used pharmaceutical hydrochlorothiazide (HCTZ), as one of the most ubiquitous contaminants in the sewage water. The study encompassed investigation of degradation by hydrolysis, photolysis, and photocatalysis employing commercially available TiO2 Degussa P25 catalyst. The process of direct photolysis and photocatalytic degradation were investigated under different type of lights...
November 6, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29108993/salvianolic-acid-b-inhibits-myofibroblast-transdifferentiation-in-experimental-pulmonary-fibrosis-via-the-up-regulation-of-nrf2
#4
Miao Liu, Hanying Xu, Ling Zhang, Cai Zhang, Liancheng Yang, Enlong Ma, Lianqing Liu, Yanchun Li
Salvianolic acid B (SalB) is one of the most bioactive components extracted from Salvia miltiorrhiza, and its antioxidant capacity corresponds with its protective effects against cell injury from oxidative stress. The aim of the present study was to evaluate the effect of SalB on experimental pulmonary fibrosis and its ability to ameliorate the oxidative/antioxidative imbalance during fibrosis pathogenesis. The anti-fibrotic activity of SalB was first confirmed in Transforming growth factor β1(TGF-β1)-stimulated MRC-5 cells...
November 4, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29108416/chlorflavonin-targets-acetohydroxyacid-synthase-catalytic-subunit-ilvb1-for-synergistic-killing-of-mycobacterium-tuberculosis
#5
Nidja Rehberg, Herve Sergi Akone, Thomas R Ioerger, German Erlenkamp, Georgios Daletos, Holger Gohlke, Peter Proksch, Rainer Kalscheuer
The flavonoid natural compound chlorflavonin was isolated from the endophytic fungus Mucor irregularis, which was obtained from the Cameroonian medicinal plant Moringa stenopetala. Chlorflavonin exhibited strong growth inhibitory activity in vitro against Mycobacterium tuberculosis (MIC90 1.56 μM) while exhibiting no cytotoxicity towards the human cell lines MRC-5 and THP-1 up to concentrations of 100 µM. Mapping of resistance-mediating mutations employing whole-genome sequencing, chemical supplementation assays, molecular docking studies as well as enzymatic characterization revealed that chlorflavonin specifically inhibits the acetohydroxyacid synthase catalytic subunit IlvB1, causing combined auxotrophies to branched chain amino acids and to pantothenic acid...
November 6, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/29107142/antibacterial-activity-and-cytotoxicity-of-pterocarpus-erinaceus-poir-extracts-fractions-and-isolated-compounds
#6
Nassifatou Koko Tittikpina, Frédéric Nana, Stéphane Fontanay, Stéphanie Philippot, Komlan Batawila, Koffi Akpagana, Gilbert Kirsch, Patrick Chaimbault, Claus Jacob, Raphaël Emmanuel Duval
ETHNOPHARMACOLOGICAL RELEVANCE: Pterocarpus erinaceus has been chosen based on ethnobotanical surveys carried out in the Tchamba district of the Republic of Togo. AIM OF THE STUDY: Investigation of the antibacterial as well as cytotoxic activities of whole extracts, fractions and compounds isolated from the leaves, trunk bark and roots of Pterocarpus erinaceus. MATERIALS AND METHODS: Bio-guided fractionation of the raw extracts of plant parts and subsequent isolation of compounds from active fractions using normal phase open column chromatography...
November 6, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29081823/biological-and-phytochemical-investigations-on-caesalpinia-benthamiana-a-plant-traditionally-used-as-antimalarial-in-guinea
#7
Jean Loua, Mohamed Sahar Traore, Aissata Camara, Mamadou Aliou Balde, Louis Maes, Luc Pieters, Aliou Mamadou Balde
Caesalpinia benthamiana is widely used as antimalarial in Guinean traditional medicine. Leaf extracts of the plant were tested for their in vitro antiprotozoal activity against Trypanosoma brucei brucei and T. cruzi and the chloroquine-sensitive Ghana strain of Plasmodium falciparum along with their cytotoxicity on MRC-5 cells. The methanolic extract showed the strongest antiprotozoal activity against P. falciparum (IC50 4 μg/ml), a good activity against T. brucei (IC50 13 μg/ml), and a moderate activity against T...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/29066297/medicinal-mascarene-aloes-an-audit-of-their-phytotherapeutic-potential
#8
D Lobine, I Cummins, J Govinden-Soulange, M Ranghoo-Sanmukhiya, K Lindsey, P L Chazot, C A Ambler, S Grellscheid, G Sharples, N Lall, I A Lambrechts, C Lavergne, M-J R Howes
A phytochemical and biological investigation of the endemic Mascarene Aloes (Aloe spp.), including A. tormentorii (Marais) L.E.Newton & G.D.Rowley, A. purpurea Lam, A. macra Haw., A. lomatophylloides Balf.f and A. vera (synonym A. barbadensis Mill.), which are used in the traditional folk medicine of the Mascarene Islands, was initiated. Methanolic extracts of the Aloes under study were analysed using high resolution LC-UV-MS/MS and compounds belonging to the class of anthraquinones, anthrones, chromones and flavone C-glycosides were detected...
October 21, 2017: Fitoterapia
https://www.readbyqxmd.com/read/29046461/human-embryonic-stem-cell-derived-neurons-are-highly-permissive-for-varicella-zoster-virus-lytic-infection
#9
Tomohiko Sadaoka, Cindi L Schwartz, Labchan Rajbhandari, Arun Venkatesan, Jeffrey I Cohen
Varicella-zoster virus (VZV) is highly cell-associated when grown in culture and has a much higher (4,000 to 20,000-fold increased) particle-to-plaque forming unit (PFU) ratio in vitro than herpes simplex virus (HSV). In contrast, VZV is highly infectious in vivo by airborne transmission. Neurons are major targets for VZV in vivo, in which the virus can establish latency and reactivate to produce infectious virus. Using neurons derived from human embryonic stem cells (hESC) and cell-free wild-type (WT) VZV, we demonstrate that neurons are nearly 100 times more permissive for WT VZV infection than very early passage human embryonic lung cells or MRC-5 diploid human fibroblasts, the cells used for vaccine production or virus isolation...
October 18, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29029807/binding-and-neutralizing-anti-cytomegalovirus-activities-in-immune-globulin-products
#10
Xiao Wang, Yanqun Xu, Dorothy E Scott, Haruhiko Murata, Evi B Struble
Congenital infection as well as infection of immunocompromised individuals by cytomegalovirus (CMV) can be associated with significant morbidity, mortality, and long-term adverse health outcomes. Assessment of anti-viral activity using appropriate assays is essential for ensuring safe and efficacious use of therapeutic CMV immune globulin (IG) products. In this study, we used commercial ELISA kits to compare anti-CMV antibody binding activity and avidity for lots of CMV-specific and normal IG products available in the US market...
October 10, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/29029734/3d-lung-spheroid-cultures-for-evaluation-of-photodynamic-therapy-pdt-procedures-in-microfluidic-lab-on-a-chip-system
#11
Agnieszka Zuchowska, Elzbieta Jastrzebska, Michal Chudy, Artur Dybko, Zbigniew Brzozka
The purpose of this paper is to present a fully integrated microchip for the evaluation of PDT procedures efficiency on 3D lung spheroid cultures. Human lung carcinoma A549 and non-malignant MRC-5 spheroids were utilized as culture models. Spheroid viability was evaluated 24 h after PDT treatment, in which 5-aminolevulinic acid (ALA) had been used as a precursor of a photosensitizer (protoporphyrin IX - PpIX). Moreover, spheroid viability over a long-term (10-day) culture was also examined. We showed that the proposed PDT treatment was toxic only for cancer spheroids...
October 16, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29027187/midterm-results-of-trochanteric-flip-osteotomy-technique-for-management-of-fractures-around-the-hip
#12
Vivek Trikha, Saubhik Das, Arkesh Madegowda, Prabhat Agrawal
INTRODUCTION: In this study, we aimed to investigate safety and efficacy of the trochanteric flip osteotomy with surgical hip dislocation technique in selected displaced acetabular and femoral head fractures with clinico-radiological outcome and potential complications. MATERIALS AND METHODS: We retrospectively reviewed 32 patients from January 2009 to June 2014. Selected displaced acetabular fractures with comminution and/or cranial extension of posterior wall, marginal impaction, intraarticular fragment, femoral head fractures and hip fracture-dislocations were operated by this modified approach of trochanteric flip osteotomy and surgical hip dislocation...
October 9, 2017: Hip International: the Journal of Clinical and Experimental Research on Hip Pathology and Therapy
https://www.readbyqxmd.com/read/29024500/tumor-suppressive-roles-of-eugenol-in-human-lung-cancer-cells
#13
Li Fangjun, Yang Zhijia
BACKGROUND: Eugenol, a natural compound available in Syzigium aromaticum (cloves), is exploited for various medicinal applications. Eugenol induces apoptosis and functions as an anti-cancer drug in several types of tumors. We investigated the tumor suppressive role and potential mechanisms of eugenol in human lung cancer cells. METHODS: Human embryonic lung fibroblast MRC-5 and lung cancer adenocarcinoma A549 cells were incubated with or without various concentrations of eugenol for 24 hours...
October 12, 2017: Thoracic Cancer
https://www.readbyqxmd.com/read/28991639/haemanthamine-alters-sodium-butyrate-induced-histone-acetylation-p21-waf1-cip1-expression-chk1-and-chk2-activation-and-leads-to-increased-growth-inhibition-and-death-in-a2780-ovarian-cancer-cells
#14
Martina Seifrtová, Radim Havelek, Lucie Cahlíková, Daniela Hulcová, Naděžda Mazánková, Martina Řezáčová
BACKGROUND: Haemanthamine (HA) and sodium butyrate (NaB) are promising candidates for chemotherapy as a treatment for cancer. PURPOSE: We aimed to determine the anticancer potential of HA and NaB, alone and in combination, in A2780 ovarian cancer cells and concurrently investigated anticancer potential in contrast to non-cancer human MRC-5 fibroblasts. METHODS: Antiproliferative effects were determined by WST-1 assay and by Trypan blue exclusion staining...
November 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28988750/design-synthesis-and-biological-evaluation-of-2-phenylquinoline-4-carboxamide-derivatives-as-a-new-class-of-tubulin-polymerization-inhibitors
#15
Li Zhu, Kaixiu Luo, Ke Li, Yi Jin, Jun Lin
A novel series of 2-phenylquinoline-4-carboxamide derivatives was synthesized, characterized and evaluated for its antiproliferative activity against five cancer cell lines, Hela, SK-OV-3, HCT116, A549 and MDA-MB-468, and a normal human fetal lung fibroblastic cell line, MRC-5. Among them, compound 7b displayed potent cytotoxic activity in vitro against SK-OV-3 and HCT116 cell lines with IC50 values of 0.5 and 0.2μM, respectively. In general, the antiproliferative activity was correlated with the binding property of the colchicine binding site and inhibitory effect on tubulin polymerization...
November 1, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28987794/the-excitotoxity-of-nmda-receptor-nr2d-subtype-mediates-human-fetal-lung-fibroblasts-proliferation-and-collagen-production
#16
MingJie Wang, ZiQiang Luo, Yinyan Yue, YanRui Wang, Shu Wu, ChuanDing Cao, ZhengChang Liao, XiaoHe Yu, ShaoJie Yue
Studies have suggested that endogenous glutamate and N-methyl-d-aspartate (NMDA) receptor have an excitotoxity role during acute lung injury. Fibroblasts play a critical role in lung development and chronic lung disease after acute lung injury. This study aims to explore the immediate role of NMDAR activation in human lung fibroblasts. The expression of NMDAR 1 subtype (NR1) and four individual NMDAR 2 (NR2) subtypes (NR 2 A to D) was measured in human fetal lung fibroblasts (HFL-1 and MRC-5). Five NMDARs expression were all detectable in two cell lines...
October 5, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28970485/the-huc-mscs-cell-line-ccrc-1-represents-a-novel-safe-and-high-yielding-hdcs-for-the-production-of-human-viral-vaccines
#17
Ping Chen, Ke-Hua Zhang, Tao Na, Lin Wang, Wei-Dong Yin, Bao-Zhu Yuan, Jun-Zhi Wang
MRC-5 represents the most frequent human diploid cells (HDCs)-type cell substrate in the production of human viral vaccines. However, early-passage MRC-5 is diminishing and, due to both technical and ethical issues, it is extremely difficult to derive novel HDCs from fetal lung tissues, which are the common sources of HDCs. Our previous studies suggested that human umbilical cord may represent an alternative but convenient source of new HDCs. Here, we established a three-tiered cell banking system of a hUC-MSC line, designated previously as Cell Collection and Research Center-1 (CCRC-1)...
October 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28958531/phytochemical-composition-and-in-vitro-functional-properties-of-three-wild-rose-hips-and-their-traditional-preserves
#18
Jelena D Nađpal, Marija M Lesjak, Zorica O Mrkonjić, Tatjana M Majkić, Dragana D Četojević-Simin, Neda M Mimica-Dukić, Ivana N Beara
The aim of the present study was investigation of the phenolic profile, ascorbic acid content, antioxidant, anti-acetylcholinesterase, anti-inflammatory and cytotoxic activity of rose hips and the preserves (purée and jam) of three insufficiently examined Rosa species: Rosa dumalis Bechst., R. dumetorum Thuill. and R. sempervirens L. The liquid chromatography-tandem mass spectrometry analysis resulted in quantification of 14 of the 45 phenolic compounds examined, with ellagic acid as the most dominant. Notable antioxidant activity of all three species was confirmed through several assays...
February 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/28946027/synthesis-crystal-structure-and-antiproliferative-mechanisms-of-2-acetylpyridine-thiosemicarbazones-ga-iii-with-a-greater-selectivity-against-tumor-cells
#19
Jinxu Qi, Yunyun Zheng, Kun Qian, Liang Tian, Guo-Xin Zhang, Zhen Cheng, Yihong Wang
Thiosemicarbazone Ga(III) complexes (C3-C5) were synthesized and characterized by X-ray single crystal diffraction, and they were all 1:1 ligand/Ga(III) complexes. The antiproliferative activity of these Ga(III) complexes was tested against three cancer cell lines, demonstrating that Ga(III) complexes showed about 3-10 folds more anticancer activity than their ligands alone. Importantly, thiosemicarbazones and Ga(III) complexes have a low toxicity to human fetal lung fibroblast cells (MRC-5) and exhibit a high therapeutic index for tumor cells...
September 19, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28925484/mir-22-inhibits-lung-cancer-cell-emt-and-invasion-through-targeting-snail
#20
K Zhang, X-Y Li, Z-M Wang, Z-F Han, Y-H Zhao
OBJECTIVE: Snail is an important factor in regulating epithelial mesenchymal transition (EMT). Its elevation is related to the enhancement of lung cancer invasion. MicroRNA-22 (MiR-22) plays a role in regulating lung cancer cell invasion. Bioinformatics analysis showed the complementary binding site between miR-22 and Snail. This study aimed to investigate the role of miR-22 in regulating Snail and affecting lung cancer cell invasion and metastasis. MATERIALS AND METHODS: Dual luciferase assay confirmed the targeted relationship between miR-22 and Snail...
August 2017: European Review for Medical and Pharmacological Sciences
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