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Calcium channel subunit

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https://www.readbyqxmd.com/read/29466724/bk-potassium-channels-suppress-cav%C3%AE-2%C3%AE-subunit-function-to-reduce-inflammatory-and-neuropathic-pain
#1
Fang-Xiong Zhang, Vinicius M Gadotti, Ivana A Souza, Lina Chen, Gerald W Zamponi
Cavα2δ subunits contribute to the cell-surface expression of Cav2 calcium channels. Upregulation of Cavα2δ-1 in dorsal root ganglion neurons occurs after nerve injury and results in an increased synaptic abundance of Cav2.2 channels in the spinal dorsal horn, thus enhancing the transmission of pain signals. Here, we report that large conductance calcium-activated potassium (BK) channels interact with the Cavα2δ subunit. Coexpression of BK channels with the Cav2 calcium channels reduces their cell-surface expression and whole-cell current density by competing the Cavα2δ subunit away from the Cav2 complex...
February 20, 2018: Cell Reports
https://www.readbyqxmd.com/read/29459734/the-ca-v-2%C3%AE-1-ef-hand-f-helix-tyrosine-a-highly-conserved-locus-for-gpcr-inhibition-of-ca-v-2-channels
#2
Tyler W Dunn, Xiaotang Fan, Ariel R Ase, Philippe Séguéla, Wayne S Sossin
The sensory neuron of Aplysia californica participates in several forms of presynaptic plasticity including homosynaptic depression, heterosynaptic depression, facilitation and the reversal of depression. The calcium channel triggering neurotransmitter release at most synapses is CaV 2, consisting of the pore forming α1 subunit (CaV 2α1), and auxiliary CaV β, and CaV α2δ subunits. To determine the role of the CaV 2 channel in presynaptic plasticity in Aplysia, we cloned Aplysia CaV 2α1, CaV β, and CaV α2δ and over-expressed the proteins in Aplysia sensory neurons (SN)...
February 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29458039/differential-contribution-of-calcium-channels-to-%C3%AE-1-adrenoceptor-mediated-contraction-is-responsible-for-diverse-responses-to-cooling-between-rat-tail-and-iliac-arteries
#3
Hirotake Ishida, Shin-Ya Saito, Eita Hishinuma, Tomoaki Kitayama, Tomohisa Ishikawa
Our previous studies have shown that α1 -adrenoceptors, in addition to α2 -adrenoceptors, are involved in enhanced contraction of cutaneous blood vessels during cooling. The present study aimed to elucidate the mechanism underlying it. In tail and iliac arteries isolated from rats, isometric contraction was measured using a myograph and the phosphorylation level of myosin phosphatase target subunit 1 (MYPT1) was quantified by western blotting. The phenylephrine-induced contraction was enhanced by cooling to 24°C in tail arteries, but was suppressed in iliac arteries...
February 16, 2018: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29448101/neuronal-calcium-channel-%C3%AE-1-subunit-interacts-with-ampa-receptor-increasing-its-cell-surface-localisation
#4
Bo Am Seo, Jung-Ha Lee, Ho Min Kim, Myoung-Goo Kang
Voltage-activated Ca 2+ channels (Ca v ) play critical roles in excitable cells including neurons. Unlike the well-defined roles of Ca v 2 for pre-synaptic neurotransmission, the post-synaptic function of Ca v 2 is unclear. Based on our previous study demonstrating the postsynaptic association of the Ca v 2 with the AMPA receptor (AMPA-R), in this study we sought to further analyse the Ca v 2-AMPA-R association. We used a step-by-step dissociation of partially purified native Ca v 2-AMPA-R complexes and co-immunoprecipitation of the Ca v 2-AMPA-R complexes expressed in HEK293T cells to demonstrate that the main subunit of Ca v , α 1 , formed a complex with the AMPA-R without the auxiliary subunits β, α 2 δ, γ 2/3 ...
February 12, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29444988/nmda-type-glutamate-receptor-activation-promotes-vascular-remodeling-and-pulmonary-arterial-hypertension
#5
Sébastien J Dumas, Gilles Bru-Mercier, Audrey Courboulin, Marceau Quatredeniers, Catherine Rücker-Martin, Fabrice Antigny, Morad K Nakhleh, Benoit Ranchoux, Elodie Gouadon, Maria-Candida Vinhas, Matthieu Vocelle, Nicolas Raymond, Peter Dorfmüller, Elie Fadel, Frédéric Perros, Marc Humbert, Sylvia Cohen-Kaminsky
Background -Excessive proliferation and apoptosis resistance in pulmonary vascular cells underlie vascular remodeling in pulmonary arterial hypertension (PAH). Specific treatments for PAH exist, mostly targeting endothelial dysfunction, but high pulmonary arterial pressure still causes heart failure and death. Pulmonary vascular remodeling may be driven by metabolic reprogramming of vascular cells to increase glutaminolysis and glutamate production. The N-methyl-D-aspartate receptor (NMDAR), a major neuronal glutamate receptor, is also expressed on vascular cells, but its role in PAH is unknown...
February 14, 2018: Circulation
https://www.readbyqxmd.com/read/29439248/transient-receptor-potential-vanilloid-4-activation-induced-increase-in-glycine-activated-current-in-mouse-hippocampal-pyramidal-neurons
#6
Mengwen Qi, Chunfeng Wu, Zhouqing Wang, Li Zhou, Chen Men, Yimei Du, Songming Huang, Lei Chen, Ling Chen
BACKGROUND/AIMS: Glycine plays an important role in regulating hippocampal inhibitory/ excitatory neurotransmission through activating glycine receptors (GlyRs) and acting as a co-agonist of N-methyl-d-aspartate-type glutamate receptors. Activation of transient receptor potential vanilloid 4 (TRPV4) is reported to inhibit hippocampal A-type γ-aminobutyric acid receptor, a ligand-gated chloride ion channel. GlyRs are also ligand-gated chloride ion channels and this paper aimed to explore whether activation of TRPV4 could modulate GlyRs...
February 7, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29437792/mechanistic-exploration-of-cancer-stem-cell-marker-voltage-dependent-calcium-channel-%C3%AE-2%C3%AE-1-subunit-mediated-chemotherapy-resistance-in-small-cell-lung-cancer
#7
Jiangyong Yu, Shuhang Wang, Wei Zhao, Jianchun Duan, Zhijie Wang, Hanxiao Chen, Yanhua Tian, Di Wang, Jun Zhao, Tongtong An, Hua Bai, Meina Wu, Jie Wang
PURPOSE: Chemo-resistance in small cell lung cancer (SCLC) is reportedly attributed to the existence of resistant cancer stem cells (CSCs). Studies involving CSC-specific markers and related mechanisms in SCLC remain limited. The current study explored the role of the voltage-dependent calcium channel α2δ1 subunit as a CSC marker in chemo-resistant SCLC, and explored the potential mechanisms of α2δ1-mediated chemo-resistance and strategies of overcoming the resistance. EXPERIMENTAL DESIGN: α2δ1 positive cells were identified and isolated from SCLC cell lines and patient derived xenografts (PDXs) models, and CSC-like properties were subsequently verified...
February 6, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29434054/influence-of-dutasteride-and-nifedipine-on-catsper-gene-expression-in-epididymis-of-mice
#8
Archana Srivastav, Bendangla Changkija, Kunal Sharan, Geet Kumar Nagar, Falgun Wanganuji Bansode
Calcium (Ca2+) signaling is critical for successful fertilization. In spermatozoa, capacitation, hyperactivation of motility and the acrosome reaction are all mediated by increases in intracellular Ca2 + through CatSper (sperm specific cation channel). The CatSper channel complex contains four pore forming α subunits (CatSper1-4 ) and five accessory subunits called β, δ, ε, γ and ζ . Genetic deletion of any of the four CatSper genes in mice results in loss of hyperactivated motility and male infertility...
February 6, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29427102/spinocerebellum-ataxia-type-6-molecular-mechanisms-and-calcium-channel-genetics
#9
Xiaofei Du, Christopher Manuel Gomez
Spinocerebellar ataxia (SCA) type 6 is an autosomal dominant disease affecting cerebellar degeneration. Clinically, it is characterized by pure cerebellar dysfunction, slowly progressive unsteadiness of gait and stance, slurred speech, and abnormal eye movements with late onset. Pathological findings of SCA6 include a diffuse loss of Purkinje cells, predominantly in the cerebellar vermis. Genetically, SCA6 is caused by expansion of a trinucleotide CAG repeat in the last exon of longest isoform CACNA1A gene on chromosome 19p13...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29425964/alterations-of-l-type-voltage-dependent-calcium-channel-alpha-1-subunit-in-the-hippocampal-ca3-region-during-and-after-pilocarpine-induced-epilepsy
#10
Jie Hua Xu, Hui Wang, Wen Zhang, Feng Ru Tang
Voltage-dependent calcium channels (VDCC) have been shown to regulate neuronal excitability and their antagonists have been used clinically for the control of seizures. While functional studies of VDCC in epileptogenesis in the CA1 area of hippocampus or the dentate gyrus have been done, few studies were carried out in the CA3 area. Given the bursting characteristics of the CA3 neurons, we speculated that VDCC in the CA3 area might play an important role in the epileptogenesis. In the present study in the mouse pilocarpine model of temporal lobe epilepsy, we investigated the alterations of alpha 1 subunits of L-type VDCC in the CA3 area of the hippocampus at different stages of epileptogenesis, i...
February 7, 2018: Neurochemistry International
https://www.readbyqxmd.com/read/29423686/tgf-%C3%AE-1-regulation-of-p-jnk-and-l-type-calcium-channel-cav1-2-in-cortical-neurons
#11
Zhenning Liu, Jiangtao Sheng, Guoyi Peng, Jinhua Yang, Weiqiang Chen, Kangsheng Li
Central nervous system (CNS) diseases can cause a series of neuronal lesions, which may be improved by the anti-apoptotic neuroprotection of transforming growth factor-beta 1 (TGF-β1). In neurons, L-type Ca2+ channels (LTCC) are mainly composed of Cav1.2 subunits. Given the implication of TGF-β1 in numerous CNS diseases, we examined the neuroprotective effects of TGF-β1 on the Cav1.2 channel in the CNS. To simulate acute mechanical traumatic brain injury (TBI), we used a needle to create parallel scratches across plates, which were cultured for 9 h...
February 8, 2018: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/29422840/mechanisms-underlying-serotonergic-excitation-of-callosal-projection-neurons-in-the-mouse-medial-prefrontal-cortex
#12
Emily K Stephens, Arielle L Baker, Allan T Gulledge
Serotonin (5-HT) selectively excites subpopulations of pyramidal neurons in the neocortex via activation of 5-HT2A (2A) receptors coupled to Gq subtype G-protein alpha subunits. Gq-mediated excitatory responses have been attributed primarily to suppression of potassium conductances, including those mediated by KV7 potassium channels (i.e., the M-current), or activation of non-specific cation conductances that underlie calcium-dependent afterdepolarizations (ADPs). However, 2A-dependent excitation of cortical neurons has not been extensively studied, and no consensus exists regarding the underlying ionic effector(s) involved...
2018: Frontiers in Neural Circuits
https://www.readbyqxmd.com/read/29420074/extracellular-acidosis-suppresses-calcification-of-vascular-smooth-muscle-cells-by-inhibiting-calcium-influx-via-l-type-calcium-channels
#13
Shenglei Zhang, Jinsheng Xu, Yu Feng, Junxia Zhang, Liwen Cui, Huiran Zhang, Yaling Bai
Vascular calcification such as arteriosclerosis, which is characterized by a calcification of the tunica media, is a severe complication of chronic kidney disease (CKD), contributing to the high prevalence of cardiovascular morbidity and mortality in patients with CKD. An essential step during the development of arteriosclerosis is the transdifferentiation/calcification of vascular smooth muscle cells (VSMCs), resembling osteogenesis. Metabolic acidosis, a common clinical manifestation in CKD, is known to decrease vascular calcification...
February 8, 2018: Clinical and Experimental Hypertension: CHE
https://www.readbyqxmd.com/read/29401454/biophysical-attributes-that-affect-camkii-activation-deduced-with-a-novel-spatial-stochastic-simulation-approach
#14
Ximing Li, William R Holmes
Calcium/calmodulin-dependent protein kinase II (CaMKII) holoenzymes play a critical role in decoding Ca2+ signals in neurons. Understanding how this occurs has been the focus of numerous studies including many that use models. However, CaMKII is notoriously difficult to simulate in detail because of its multi-subunit nature, which causes a combinatorial explosion in the number of species that must be modeled. To study the Ca2+-calmodulin-CaMKII reaction network with detailed kinetics while including the effect of diffusion, we have customized an existing stochastic particle-based simulator, Smoldyn, to manage the problem of combinatorial explosion...
February 5, 2018: PLoS Computational Biology
https://www.readbyqxmd.com/read/29386119/ip3-receptor-dependent-cytoplasmic-ca2-signals-are-tightly-controlled-by-cav%C3%AE-3
#15
Anouar Belkacemi, Xin Hui, Barbara Wardas, Matthias W Laschke, Ulrich Wissenbach, Michael D Menger, Peter Lipp, Andreas Beck, Veit Flockerzi
Voltage-gated calcium channels (Cavs) are major Ca2+ entry pathways in excitable cells. Their β subunits facilitate membrane trafficking of the channel's ion-conducting α1 pore and modulate its gating properties. We report that one β subunit, β3, reduces Ca2+ release following stimulation of phospholipase C-coupled receptors and inositol 1,4,5-trisphosphate (IP3) formation. This effect requires the SH3-HOOK domain of Cavβ3, includes physical β3/IP3 receptor interaction, and prevails when agonist-induced IP3 formation is bypassed by photolysis of caged IP3...
January 30, 2018: Cell Reports
https://www.readbyqxmd.com/read/29385621/outgroup-emotion-processing-in-the-vacc-is-modulated-by-childhood-trauma-and-cacna1c-risk-variant
#16
Johannes T Krautheim, Benjamin Straube, Udo Dannlowski, Martin Pyka, Henriette Schneider-Hassloff, Rebecca Drexler, Axel Krug, Jens Sommer, Marcella Rietschel, Stephanie H Witt, Tilo Kircher
A high frequency of outgroup contact - as experienced by urban dwellers and migrants - possibly increases schizophrenia risk. This risk might be further amplified by genetic and environmental risk factors, such as the A-allele of rs1006737 within the Calcium Voltage-Gated Channel Subunit Alpha1 C (CACNA1C) gene and Childhood Interpersonal Trauma (CIT). Both have been related to ventral anterior cingulate cortex (vACC) functioning. We investigated vACC functioning, during ingroup and outgroup emotion perception in relation to rs1006737 and CIT...
January 29, 2018: Social Cognitive and Affective Neuroscience
https://www.readbyqxmd.com/read/29371110/intramembranal-disulfide-cross-linking-elucidates-the-super-quaternary-structure-of-mammalian-catspers
#17
Christopher Bystroff
CatSper is a voltage-dependent calcium channel located in the plasma membrane of the sperm flagellum and is responsible for triggering hyperactive motility. A homology model for the transmembrane region was built in which the arrangement of the subunits around the pseudo-four-fold symmetry axis was deduced by the pairing of conserved transmembranal cysteines across mammals. Directly emergent of the predicted quaternary structure is an architecture in which tetramers polymerize through additional, highly conserved cysteines, creating one or more double-rows channels extending the length of the principal piece of the mammalian sperm tail...
January 19, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29356177/proline-rich-transmembrane-protein-2-negative-paroxysmal-kinesigenic-dyskinesia-clinical-and-genetic-analyses-of-163-patients
#18
Wo-Tu Tian, Xiao-Jun Huang, Xiao Mao, Qing Liu, Xiao-Li Liu, Sheng Zeng, Xia-Nan Guo, Jun-Yi Shen, Yang-Qi Xu, Hui-Dong Tang, Xiao-Meng Yin, Mei Zhang, Wei-Guo Tang, Xiao-Rong Liu, Bei-Sha Tang, Sheng-Di Chen, Li Cao
BACKGROUND: Paroxysmal kinesigenic dyskinesia is the most common type of paroxysmal dyskinesia. Approximately half of the cases of paroxysmal kinesigenic dyskinesia worldwide are attributable to proline-rich transmembrane protein 2 mutations. OBJECTIVE: The objective of this study was to investigate potential causative genes and clinical characteristics in proline-rich transmembrane protein 2-negative patients with paroxysmal kinesigenic dyskinesia. METHODS: We analyzed clinical manifestations and performed exome sequencing in a cohort of 163 proline-rich transmembrane protein 2-negative probands, followed by filtering data with a paroxysmal movement disorders gene panel...
January 22, 2018: Movement Disorders: Official Journal of the Movement Disorder Society
https://www.readbyqxmd.com/read/29355786/upregulation-of-calcium-channel-alpha-2-delta-1-subunit-in-dorsal-horn-contributes-to-spinal-cord-injury-induced-tactile-allodynia
#19
Kazuki Kusuyama, Toshiya Tachibana, Hiroki Yamanaka, Masamichi Okubo, Shinichi Yoshiya, Koichi Noguchi
BACKGROUND CONTEX: Spinal cord injury (SCI) commonly results not only in motor paralysis but also in the emergence of neuropathic pain, both of which can impair the quality of life for SCI patients. In the clinical field, it is well known that pregabalin, which binds to the voltage-gated calcium channel α2δ-1subunit has therapeutic effects on neuropathic pain after SCI. A Previous study has demonstrated that SCI increased α2δ-1 in L4-6 dorsal spinal cord of SCI rats by Western blot analysis and that the increase of α2δ-1 was correlated with tactile allodynia of the hind paw...
January 16, 2018: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/29338087/thrombospondin-4-acts-on-pre-synaptic-calcium-channel-alpha-2-delta-1-subunit-to-induce-synaptogenesis-between-sensory-and-spinal-cord-neurons-through-a-t-type-calcium-channel-dependent-pathway-that-is-blocked-by-early-low-dose-gabapentin-treatments
#20
Yanhui Peter Yu, Nian Gong, Tae Dong Kweon, Benjamin Vo, Z David Luo
BACKGROUND AND PURPOSE: Nerve injury induces concurrent upregulation of calcium channel alpha-2-delta-1 subunit (Cav α2 δ1 ) and extracellular matrix protein thrombospondin-4 (TSP4) in dorsal root ganglia and dorsal spinal cord, leading to neuropathic pain state development. Interactions of these proteins promote aberrant excitatory synaptogenesis that contributes to neuropathic pain state development through unknown mechanisms. We investigated the contributory role of Cav α2 δ1 /TSP4 to synaptogenesis, and pathway involved in vitro, and whether gabapentin could block this process and pain development in vivo...
January 16, 2018: British Journal of Pharmacology
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