Rong Guo, Xiaotao Dong, Feng Chen, Tianrong Ji, Qiannan He, Jie Zhang, Yingliang Sheng, Yanjiang Liu, Shengxiong Yang, Weifang Liang, Yawei Song, Ke Fang, Lingling Zhang, Gongcheng Hu, Hongjie Yao
The transition of mouse embryonic stem cells (ESCs) between serum/LIF and 2i(MEK and GSK3 kinase inhibitor)/LIF culture conditions serves as a valuable model for exploring the mechanisms underlying ground and confused pluripotent states. Regulatory networks comprising core and ancillary pluripotency factors drive the gene expression programs defining stable naïve pluripotency. In our study, we systematically screened factors essential for ESC pluripotency, identifying TEAD2 as an ancillary factor maintaining ground-state pluripotency in 2i/LIF ESCs and facilitating the transition from serum/LIF to 2i/LIF ESCs...
April 11, 2024: EMBO Journal