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https://www.readbyqxmd.com/read/29786424/tuning-gene-activity-by-inducible-and-targeted-regulation-of-gene-expression-in-minimal-bacterial-cells
#1
Ana M Mariscal, Shigeyuki Kakizawa, Jonathan Y Hsu, Kazuki Tanaka, Luis González-González, Alicia Broto, Enrique Querol, Maria Lluch-Senar, Carlos Piñero-Lambea, Lijie Sun, Philip D Weyman, Kim S Wise, Chuck Merryman, Gavin Tse, Adam J Moore, Clyde A Hutchison, Hamilton O Smith, Masaru Tomita, J Craig Venter, John I Glass, Jaume Piñol, Yo Suzuki
Functional genomics studies in minimal mycoplasma cells enable unobstructed access to some of the most fundamental processes in biology. Conventional transposon bombardment and gene knockout approaches often fail to reveal functions of genes that are essential for viability, where lethality precludes phenotypic characterization. Conditional inactivation of genes is effective for characterizing functions central to cell growth and division, but tools are limited for this purpose in mycoplasmas. Here we demonstrate systems for inducible repression of gene expression based on clustered regularly interspaced short palindromic repeats-mediated interference (CRISPRi) in Mycoplasma pneumoniae and synthetic Mycoplasma mycoides, two organisms with reduced genomes actively used in systems biology studies...
May 22, 2018: ACS Synthetic Biology
https://www.readbyqxmd.com/read/29782909/charge-clusters-signatures-in-prokaryotic-proteomes-temperature-dependence-and-distribution
#2
Najla Kharrat, Rahil Boumellasa, Sabrine Belmabrouk, Riadh Benmarzoug, Ahmed Rebai
The identification and the screening of Charged Clusters (CCs) residues in proteins is a key analysis to assess any quantitative structure-function correlation in proteins. Here, we present a proteome wide scan for the occurrence of (CCs) in 99292 proteins using a new tool, Finding Clusters Charge in Protein Sequences Program (FCCP). The FCCP has been employed to search CCs in 35 prokaryotic proteomes (7 Psychrophiles, 10 Mesophiles, 9 thermophiles and for 9 hyperthermophiles). A new repository of 855 CC has been created...
May 18, 2018: Genomics
https://www.readbyqxmd.com/read/29782156/combining-high-throughput-sequencing-of-sedadna-and-traditional-palaeolimnological-techniques-to-infer-historical-trends-in-cyanobacterial-communities
#3
Timothy J Tse, Lorne E Doig, Song Tang, Xiaohui Zhang, Weimin Sun, Steve B Wiseman, Cindy Xin Feng, Hongling Liu, John P Giesy, Markus Hecker, Paul D Jones
Freshwaters worldwide are under increasing pressure from anthropogenic activities and changing climate. Unfortunately, many inland waters lack sufficient long-term monitoring to assess environmental trends. Analysis of sedimentary ancient DNA (sedaDNA) is emerging as a means to reconstruct the past occurrence of microbial communities of inland waters. The purpose of this study was to assess a combination of high-throughput sequencing (16S rRNA) of sedaDNA and traditional palaeolimnological analyses to explore multidecadal relationships among cyanobacterial community composition, the potential for cyanotoxin production and palaeoenvironmental proxies...
May 21, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29779159/an-etiological-approach-to-sexual-offender-assessment-case-formulation-incorporating-risk-assessment-cafira
#4
REVIEW
Leam A Craig, Martin Rettenberger
PURPOSE OF REVIEW: Case formulations (CF) have been the cornerstone of effective practice in clinical psychology since the 1950s and now form one of the core competencies in clinical and forensic assessment. The use of CFs within forensic settings is becoming more relevant when working with offenders who have experienced significant trauma, suffered from personality disorder, and have displayed sexually abusive behavior. Furthermore, most North American and European jurisdictions insist that expert witnesses adopt an idiosyncratic approach to risk assessment and consider the characteristics of the individual as part of a wider formulation of the problem behavior...
May 19, 2018: Current Psychiatry Reports
https://www.readbyqxmd.com/read/29779150/disorienting-dilemmas-the-significance-of-resistance-and-disturbance-in-an-intercultural-program-within-kindergarten-teacher-education
#5
Åsta Birkeland, Siv Ødemotland
As the Norwegian society, and thereby the kindergartens, have become more multicultural, the need for cultivating teachers capable of operating in an ever diversified and global world is highlighted as an important educational strategy within teacher education. The purpose of the specific intercultural program in kindergarten teacher education discussed in this article refers to competences needed as a professional teacher in a multicultural kindergarten. Teachers often have various assumptions and beliefs taken for granted...
May 19, 2018: Integrative Psychological & Behavioral Science
https://www.readbyqxmd.com/read/29776710/pharmacokinetics-pharmacodynamics-computer-decision-support-technologies-and-antimicrobial-stewardship-the-compass-and-rudder
#6
REVIEW
Robert C Owens, Catharine C Bulik, David R Andes
The first guidelines for conducting antimicrobial stewardship in the hospitalized setting were published in 2007. These guidelines recommend that stewardship programs employ the science of pharmacokinetics-pharmacodynamics (PK-PD) as well as adopting computerized decision support technologies when possible. The United States Food and Drug Administration have adopted PK-PD as a cornerstone in the evaluation of antimicrobial agents during clinical development. The core principles of PK-PD center around describing the relationship between drug exposure indexed to the susceptibility of the infecting bacterial pathogen and patient response...
April 13, 2018: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29775949/design-and-synthesis-of-some-new-carboxamide-and-propanamide-derivatives-bearing-phenylpyridazine-as-a-core-ring-and-the-investigation-of-their-inhibitory-potential-on-in-vitro-acetylcholinesterase-and-butyrylcholinesterase
#7
Burcu Kilic, Hayrettin O Gulcan, Fatma Aksakal, Tugba Ercetin, Nihan Oruklu, E Umit Bagriacik, Deniz S Dogruer
A series of new carboxamide and propanamide derivatives bearing phenylpyridazine as a core ring were designed, synthesized and evaluated for their ability to inhibit both cholinesterase enzymes. In addition, a series of carboxamide and propanamide derivatives bearing biphenyl instead of phenylpyridazine were also synthesized to examine the inhibitory effect of pyridazine moiety on both cholinesterase enzymes. The inhibitory activity results revealed that compounds 5b, 5f, 5h, 5j, 5l pyridazine-3-carboxamide derivative, exhibited selective acetylcholinesterase (AChE) inhibition with IC50 values ranging from 0...
May 8, 2018: Bioorganic Chemistry
https://www.readbyqxmd.com/read/29775509/exploring-the-growth-trajectory-of-cultural-competence-in-taiwanese-paediatric-nurses
#8
Chiu-I Chen, Mei-Chih Huang
AIM: The purpose of this study was to explore the evolution of cultural competence in Taiwanese paediatric nurses. BACKGROUND: Because transnational marriage has become a social phenomenon in Taiwan, the proportion of newborns of new immigrant mothers accounts for 8-10% of total births every year. Since family-centered care is the core value of paediatric nursing, it is necessary to teach caregivers how to take care of hospitalized children and perform related nursing care as well as to determine what difficulties nurses will encounter when they care for patients from diverse cultures and to find solutions for these problems...
May 18, 2018: Journal of Clinical Nursing
https://www.readbyqxmd.com/read/29775171/competency-standards-for-newly-graduated-prosthetist-orthotists-in-sweden
#9
Nerrolyn Ramstrand, Simon Ramstrand
BACKGROUND: There are currently no national competency standards upon which to develop educational objectives for prosthetist/orthotists in Sweden. While standards have been developed in other countries, they cannot be applied without confirming their relevance in a Swedish context. OBJECTIVES: To describe and obtain consensus on core competencies required for newly graduated prosthetist/orthotists in Sweden. STUDY DESIGN: Modified Delphi process...
May 1, 2018: Prosthetics and Orthotics International
https://www.readbyqxmd.com/read/29775078/effects-of-a-parent-implemented-developmental-reciprocity-treatment-program-for-children-with-autism-spectrum-disorder
#10
Grace W Gengoux, Salena Schapp, Sarah Burton, Christina M Ardel, Robin A Libove, Gina Baldi, Kari L Berquist, Jennifer M Phillips, Antonio Y Hardan
Developmental approaches to autism treatment aim to establish strong interpersonal relationships through joint play. These approaches have emerging empirical support; however, there is a need for further research documenting the procedures and demonstrating their effectiveness. This pilot study evaluated changes in parent behavior and child autism symptoms following a 12-week Developmental Reciprocity Treatment parent-training program. A total of 22 children with autism spectrum disorder between 2 and 6 years (mean age = 44...
May 1, 2018: Autism: the International Journal of Research and Practice
https://www.readbyqxmd.com/read/29774603/programmed-nanoparticle-loaded-nanoparticles-for-deep-penetrating-3d-cancer-therapy
#11
Jinhwan Kim, Changshin Jo, Won-Gwang Lim, Sungjin Jung, Yeong Mi Lee, Jun Lim, Haeshin Lee, Jinwoo Lee, Won Jong Kim
Tumors are 3D, composed of cellular agglomerations and blood vessels. Therapies involving nanoparticles utilize specific accumulations due to the leaky vascular structures. However, systemically injected nanoparticles are mostly uptaken by cells located on the surfaces of cancer tissues, lacking deep penetration into the core cancer regions. Herein, an unprecedented strategy, described as injecting "nanoparticle-loaded nanoparticles" to address the long-lasting problem is reported for effective surface-to-core drug delivery in entire 3D tumors...
May 18, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29771202/analysing-healthcare-coordination-using-translational-mobilization
#12
Davina Allen
Purpose The purpose of this paper is to introduce translational mobilization theory (TMT) and explore its application for healthcare quality improvement purposes. Design/methodology/approach TMT is a generic sociological theory that explains how projects of collective action are progressed in complex organizational contexts. This paper introduces TMT, outlines its ontological assumptions and core components, and explores its potential value for quality improvement using rescue trajectories as an illustrative case...
May 21, 2018: Journal of Health Organization and Management
https://www.readbyqxmd.com/read/29770812/reduction-photo-dual-responsive-polymeric-prodrug-nanoparticles-for-programmed-sirna-and-doxorubicin-delivery
#13
Ming Wu, Jiong Li, Xinyi Lin, Zuwu Wei, Da Zhang, Bixing Zhao, Xiaolong Liu, Jingfeng Liu
Dual and multi-stimuli responsive polymeric nanoparticles that respond to two or more signals can further improve drug release performance compared with nanoparticles that respond to a single stimulus. However, usage of such nanoparticles to deliver siRNA and chemotherapeutic drugs in a sequential manner are currently very rare; meanwhile, this technology is vital to optimize the efficacy of chemotherapy towards cancer cells with multidrug resistance. By loading o-nitrobenzyl ester derivative caged DOX (DOC) into the inner poly(lactic-co-glycolic acid) (PLGA) core and adsorbing siRNA of P-gp protein onto the cationic polymeric shell derived from a disulfide-containing alkyl modified polyethylenimine (C16-S-S-PEI), here, a reduction/photo dual responsive device (RPDRD) is successfully designed for programmed P-gp siRNA and doxorubicin delivery...
May 17, 2018: Biomaterials Science
https://www.readbyqxmd.com/read/29770501/correlation-between-cycling-power-and-muscle-thickness-in-cyclists
#14
Hyung-Jin Lee, Kang-Woo Lee, Yong-Woo Lee, Hee-Jin Kim
INTRODUCTION: The aim of this study was to determine the correlation between muscle thickness (MT) and cycling power in varsity cyclists using ultrasonography (US) and to identify any differences in MT between short- and long-distance cyclists. MATERIALS AND METHODS: Twelve cyclists participated in this study. Real-time two-dimensional B-mode US was used to measure the MT in the anterior thigh, anterior lower leg, and trunk, especially in the abdominal and lumbar regions...
May 17, 2018: Clinical Anatomy
https://www.readbyqxmd.com/read/29770321/developing-workforce-capacity-in-public-health-informatics-core-competencies-and-curriculum-design
#15
Douglas R Wholey, Martin LaVenture, Sripriya Rajamani, Rob Kreiger, Craig Hedberg, Cynthia Kenyon
We describe a master's level public health informatics (PHI) curriculum to support workforce development. Public health decision-making requires intensive information management to organize responses to health threats and develop effective health education and promotion. PHI competencies prepare the public health workforce to design and implement these information systems. The objective for a Master's and Certificate in PHI is to prepare public health informaticians with the competencies to work collaboratively with colleagues in public health and other health professions to design and develop information systems that support population health improvement...
2018: Frontiers in Public Health
https://www.readbyqxmd.com/read/29769034/demand-side-determinants-of-timely-vaccination-of-oral-polio-vaccine-in-social-mobilization-network-areas-of-core-group-polio-project-in-uttar-pradesh-india
#16
Manojkumar Choudhary, Roma Solomon, Jitendra Awale, Rina Dey
BACKGROUND: Children who receive all doses of scheduled vaccines reduce their susceptibility to vaccine-preventable diseases. In India, full immunization coverage has increased significantly. However, only a small proportion of children are immunized on time. Globally, studies on factors affecting coverage of childhood immunization have found a significant impact by demand and supply-side determinants. This paper explores the demand-side determinants of timely immunization of the third dose of oral polio vaccine (OPV3) among children aged 6-11 months in the catchment areas of CORE Group Polio Project India...
May 16, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29768364/comparative-effectiveness-of-fragility-fracture-integrated-rehabilitation-management-for-elderly-individuals-after-hip-fracture-surgery-a-study-protocol-for-a-multicenter-randomized-controlled-trial
#17
Sang Yoon Lee, Jaewon Beom, Bo Ryun Kim, Seung-Kyu Lim, Jae-Young Lim
INTRODUCTION: Although it is essential to provide comprehensive rehabilitation after hip fracture to restore the patient to preoperative physical functioning, feasibility issues remain. Here, we describe a protocol for a randomized controlled trial (RCT) to evaluate the effectiveness of fragility fracture integrated rehabilitation management (FIRM) for elderly individuals after hip fracture surgery. We also examine the feasibility of applying FIRM in a chronic-care hospital or community-based setting...
May 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29765399/a-seasonal-time-series-model-based-on-gene-expression-programming-for-predicting-financial-distress
#18
Ching-Hsue Cheng, Chia-Pang Chan, Jun-He Yang
The issue of financial distress prediction plays an important and challenging research topic in the financial field. Currently, there have been many methods for predicting firm bankruptcy and financial crisis, including the artificial intelligence and the traditional statistical methods, and the past studies have shown that the prediction result of the artificial intelligence method is better than the traditional statistical method. Financial statements are quarterly reports; hence, the financial crisis of companies is seasonal time-series data, and the attribute data affecting the financial distress of companies is nonlinear and nonstationary time-series data with fluctuations...
2018: Computational Intelligence and Neuroscience
https://www.readbyqxmd.com/read/29764647/a-preceptor-competency-framework-for-pharmacists-part-2-of-a-3-part-series
#19
REVIEW
Sheila Walter, Katrina Mulherin, Craig D Cox
BACKGROUND: Pharmacy faculties in Canada are transitioning to the doctor of pharmacy degree which will require approximately one-third of curricula contact time in experiential education. Preceptors will be responsible for delivering this experiential component and many have received little or no training in how to be an effective educator. Although training is mandated through accreditation standards, competencies to serve as a foundation for preceptor development have yet to be created...
March 2018: Currents in Pharmacy Teaching & Learning
https://www.readbyqxmd.com/read/29764540/-2018-national-consensus-on-cardiopulmonary-resuscitation-training-in-china
#20
Lixiang Wang, Qingyi Meng, Tao Yu
To promote the technical training and scientific popularization of cardiopulmonary resuscitation (CPR) in China, the Cardiopulmonary Resuscitation Specialized Committee of Chinese Research Hospital Association combined with the Science Popularization Branch of the Chinese Medical Association wrote "2018 National consensus on cardiopulmonary resuscitation training in China". The formation was based on the general outline about "2016 National consensus on cardiopulmonary resuscitation in China", and to implement the important strategies included the "three pre" policy, prevention, precognition, and pre-warning, before the cardiac arrest (CA); the "three modernization" methods, standardized, diversified and individualized, during the CA; and the "three life" strategies, the rebirth, the extra and the extended, after the CA; and also combined with the concrete National conditions and clinical practice of China area...
May 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
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