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https://www.readbyqxmd.com/read/27936513/probing-the-structural-dynamics-of-the-crispr-cas9-rna-guided-dna-cleavage-system-by-coarse-grained-modeling
#1
Wenjun Zheng
In the adaptive immune systems of many bacteria and archaea, the Cas9 endonuclease forms a complex with specific guide/scaffold RNA to identify and cleave complementary target sequences in foreign DNA. This DNA targeting machinery has been exploited in numerous applications of genome editing and transcription control. However, the molecular mechanism of the Cas9 system is still obscure. Recently, high-resolution structures have been solved for Cas9 in different structural forms (e.g., unbound forms, RNA-bound binary complexes, and RNA-DNA-bound tertiary complexes, corresponding to an inactive state, a pre-target-bound state, and a cleavage-competent or product state), which offered key structural insights to the Cas9 mechanism...
December 9, 2016: Proteins
https://www.readbyqxmd.com/read/27936511/znf208-polymorphisms-associate-with-ischemic-stroke-in-southern-chinese-han-population
#2
Jianzhong Yu, Feng Zhou, Dong Luo, Nianzhen Wang, Chong Zhang, Tianbo Jin, Xiongfei Liang, Dan Yu
BACKGROUND: Ischemic stroke is one of the most common diseases with a high burden of neurological deficits, disability and death. Zinc finger protein 208 (ZNF208) was found to be involved in coronary heart disease, but little information was about its association with ischemic stroke. We performed this case-control study to elucidate the association between single-nucleotide polymorphisms (SNPs) within ZNF208 and risk of ischemic stroke in southern Chinese Han population. METHODS: A total of 799 subjects (400 cases and 399 health controls) were enrolled in this study...
December 9, 2016: Journal of Gene Medicine
https://www.readbyqxmd.com/read/27936493/new-additions-to-the-cluspro-server-motivated-by-capri
#3
Sandor Vajda, Christine Yueh, Dmitri Beglov, Tanggis Bohnuud, Scott E Mottarella, Bing Xia, David R Hall, Dima Kozakov
The heavily used protein-protein docking server ClusPro performs three computational steps as follows: (1) rigid body docking, (2) RMSD based clustering of the 1000 lowest energy structures, and (3) the removal of steric clashes by energy minimization. In response to challenges encountered in recent CAPRI targets, we added three new options to ClusPro. These are (1) accounting for Small Angle X-ray Scattering (SAXS) data in docking; (2) considering pairwise interaction data as restraints; and (3) enabling discrimination between biological and crystallographic dimers...
December 9, 2016: Proteins
https://www.readbyqxmd.com/read/27936489/follow-up-of-alcohol-consumption-after-liver-transplantation-interest-of-an-addiction-team
#4
Hélène Donnadieu-Rigole, Laetitia Olive, Bertrand Nalpas, Audrey Winter, José Ursic-Bedoya, Stéphanie Faure, Georges-Philippe Pageaux, Pascal Perney
BACKGROUND: Alcohol relapses after liver transplantation (LT) constitute a critical issue. Because there is no widely accepted definition of LT, its prevalence varies from 7 to 95% across studies. Only a severe relapse, the frequency of which is estimated to be 11 to 26%, decreases life expectancy after 5 years of LT and requires specific care. To improve the early identification of alcohol consumption among transplanted patients, liver transplant teams may be helped by input from an addiction team...
December 9, 2016: Alcoholism, Clinical and Experimental Research
https://www.readbyqxmd.com/read/27936484/gartland-type-ii-supracondylar-humerus-fractures-their-operative-treatment-and-lateral-pinning-are-increasing-a-population-based-epidemiologic-study-of-extension-type-supracondylar-humerus-fractures-in-children
#5
Juha-Jaakko Sinikumpu, Tytti Pokka, Minna Sirviö, Willy Serlo
Background Supracondylar humerus fractures are usual in children but their recent trends are unclear. Material and Methods A population-based study was performed to determine the epidemiology of childhood supracondylar humerus fractures during the decade, 2000 to 2009, in a geographic area of Oulu, Finland. Altogether, 565 extension-type supracondylar humerus fractures were included. The fractures and their treatment were analyzed according to the Gartland classification. Results Fracture incidence increased by 28%, from 50...
December 9, 2016: European Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/27936481/patients-with-ultrasound-diagnosis-of-hepatic-steatosis-are-at-high-metabolic-risk
#6
J Kälsch, H Keskin, A Schütte, T Baars, H A Baba, L P Bechmann, A Canbay, J P Sowa
Background and aims: Hepatic steatosis is the basis of non-alcoholic fatty liver disease (NALFD). Mere fat accumulation within hepatocytes is considered the mild form of NAFLD, but can progress in some patients to advanced steatohepatitis (NASH), which may lead to fibrosis, cirrhosis or hepatocellular carcinoma. However, even hepatic steatosis alone may be a risk factor for cardiovascular disease (CVD). Patients and methods: In the present real life study 106 patients from the outpatient clinic of the Department for Gastroenterology and Hepatology with either NAFLD (n = 60) or other typical diagnoses (n = 46) were included...
December 2016: Zeitschrift Für Gastroenterologie
https://www.readbyqxmd.com/read/27936479/the-galad-scoring-algorithm-based-on-afp-afp-l3-and-dcp-significantly-improves-detection-of-bclc-early-stage-hepatocellular-carcinoma
#7
J Best, H Bilgi, D Heider, C Schotten, P Manka, S Bedreli, M Gorray, J Ertle, L A van Grunsven, A Dechêne
Background: Hepatocellular carcinoma (HCC) is one of the leading causes of death in cirrhotic patients worldwide. The detection rate for early stage HCC remains low despite screening programs. Thus, the majority of HCC cases are detected at advanced tumor stages with limited treatment options. To facilitate earlier diagnosis, this study aims to validate the added benefit of the combination of AFP, the novel biomarkers AFP-L3, DCP, and an associated novel diagnostic algorithm called GALAD. Material and methods: Between 2007 and 2008 and from 2010 to 2012, 285 patients newly diagnosed with HCC and 402 control patients suffering from chronic liver disease were enrolled...
December 2016: Zeitschrift Für Gastroenterologie
https://www.readbyqxmd.com/read/27936476/necrotizing-enterocolitis-and-growth-in-preterm-infants-fed-predominantly-maternal-milk-pasteurized-donor-milk-or-preterm-formula-a-retrospective-study
#8
Paula M Sisk, Tinisha M Lambeth, Mario A Rojas, Teisha Lightbourne, Maria Barahona, Evelyn Anthony, Sam T Auringer
Objective To evaluate the association between necrotizing enterocolitis (NEC), growth, and feeding. Methods This is a retrospective study of 551 infants (birth weight ≤ 1,500 g, ≤32 weeks' gestation). NEC, Bell's stage ≥ 2, was confirmed by independent review of sentinel radiographs. Feeding type was defined as ≥ 50% maternal milk (MM), pasteurized donor human milk (PDHM), or preterm formula (PF). Demographic and clinical characteristics including growth were compared between the three groups...
December 9, 2016: American Journal of Perinatology
https://www.readbyqxmd.com/read/27936474/-three-years-follow-up-study-after-refractive-small-incision-lenticule-extraction-smile-using-500%C3%A2-khz-femtosecond-laser-in-fast-mode
#9
A Messerschmidt-Roth, W Sekundo, A Lazaridis, S Schulze
Background: The aim of this clinical trial was to check the results of laser built-in settings 3 years after ReLEx SMILE for moderate and high myopia in unselected "real life" patients. Patients and Methods: 50 myopic eyes of 27 patients were called in for examination after 3 years. 25 of these eyes with a preoperative myopia under - 6D comprised the "moderate myopia subgroup". All surgeries were performed with the 500 kHz VisuMax® femtosecond laser (Carl Zeiss Meditec AG, Jena) in the "fast mode" setting...
December 9, 2016: Klinische Monatsblätter Für Augenheilkunde
https://www.readbyqxmd.com/read/27936470/ocular-delivery-of-pacap1-27-protects-the-retina-from-ischemic-damage-in-rodents
#10
Dora Werling, Dora Reglodi, William A Banks, Therese S Salameh, Krisztina Kovacs, Timea Kvarik, Alexandra Vaczy, Laszlo Kovacs, Flora Mayer, Bese Danyadi, Emese Lokos, Andrea Tamas, Gabor Toth, Zsolt Biro, Tamas Atlasz
Purpose: Pituitary adenylate cyclase activating polypeptide (PACAP) is neuroprotective in neuronal injuries. Bilateral common carotid artery occlusion (BCCAO) causes chronic hypoperfusion-induced degeneration in the rat retina, where we proved the retinoprotective effect of intravitreal PACAP. Although this route of administration is a common clinical practice in several diseases, easier routes are clinically important. Our aim was to investigate the potential retinoprotective effects of PACAP eye drops in BCCAO-induced ischemic retinopathy...
December 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27936458/predictors-of-functional-disability-with-focus-on-activities-of-daily-living-a-community-based-follow-up-study-in-older-adults-in-india
#11
Aarti Nagarkar, Yashoda Kashikar
BACKGROUND: Disability in activities of daily living is a growing concern among older populations all over the world. India has one of the rapidly ageing populations and predicted burden of functional disability is higher for Indian older adults as compared to other ageing Asian countries. METHODS: Total 1140 aged 60 years and over participated in a baseline study. 560 of them participated in the prospective cohort study conducted in the city of Pune, India. An interview and functional assessment using a questionnaire and Pune-FAAT tool was carried out in 2013-14...
December 2, 2016: Archives of Gerontology and Geriatrics
https://www.readbyqxmd.com/read/27936453/biological-mechanisms-of-depression-following-treatment-with-interferon-for-chronic-hepatitis-c-a-critical-systematic-review
#12
REVIEW
Myrela O Machado, Giovanni Oriolo, Beatrice Bortolato, Cristiano A Köhler, Michael Maes, Marco Solmi, Iria Grande, Rocío Martín-Santos, Eduard Vieta, André F Carvalho
BACKGROUND: A significant subset of patients infected by the hepatitis C virus (HCV) develops a major depressive episode (MDE) during Interferon-alpha (IFN-α) based immunotherapy. We performed a systematic review of studies which examined biological mechanisms contributing to the onset of a MDE during IFN-α-based immunotherapy for HCV. METHODS: Major electronic databases were searched from inception up until 15th February 2016 for peer-reviewed prospective studies that had enrolled HCV infected patients who received IFN-α treatment...
November 27, 2016: Journal of Affective Disorders
https://www.readbyqxmd.com/read/27936451/onset-polarity-in-bipolar-disorder-a-strong-association-between-first-depressive-episode-and-suicide-attempts
#13
Laura Cremaschi, Bernardo Dell'Osso, Matteo Vismara, Cristina Dobrea, Massimiliano Buoli, Terence A Ketter, A Carlo Altamura
BACKGROUND: The role of onset polarity (OP) in patients with bipolar disorder (BD) has been increasingly investigated over the last few years, for its clinical, prognostic, and therapeutic implications. The present study sought to assess whether OP was associated with specific correlates, in particular with a differential suicidal risk in BD patients. METHODS: A sample of 362 recovered BD patients was dichotomized by OP: depressed (DO) or elevated onset (EO: hypomanic/manic/mixed)...
November 30, 2016: Journal of Affective Disorders
https://www.readbyqxmd.com/read/27936449/methotrexate-treated-ectopic-pregnancy-beta-human-chorionic-gonadotropin-serum-changes-as-a-success-predictor-using-a-mathematical-model-validation
#14
Aleksandra Kovaleva, Natalia Irishina, Augusto Pereira, Tatiana Cuesta-Guardiola, Luis Ortiz-Quintana
BACKGROUND: Surgical rescue of methotrexate-treated ectopic pregnancy is necessary when tubal rupture or medical therapy failure is detected during post-therapeutic monitoring. It is known that an increased beta human chorionic gonadotropin (β-hCG) concentration is the most important factor associated with treatment failure. Therefore, we suggested that relative changes in serum β-hCG could predict a successful result of medical treatment, leading to facilitation of the decision to forgo the prospect of possible surgical rescue...
October 27, 2016: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/27936447/single-incision-laparoscopic-excision-of-a-chylous-mesenteric-cyst-a-case-report
#15
Masanori Yoshimitsu, Manabu Emi, Masashi Miguchi, Hiroshi Ota, Keishi Hakoda, Ichiro Omori, Toshihiko Kohashi, Naoki Hirabayashi, Hideki Ohdan
INTRODUCTION: Chylous mesenteric cysts are rare intra-abdominal lesions located in the mesentery of the gastrointestinal tract and may extend from the base of the mesentery into the retroperitoneum. The treatment is the complete removal of the cyst PRESENTATION OF CASE: A 49-year-old female presented with abdominal pain. Abdominal computed tomography showed a 5.0-cm-diameter intraabdominal, homogenous cystic lesion located on the mesentery of the small intestine. Single-incision laparoscopic surgery was performed for complete resection...
November 22, 2016: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/27936446/design-synthesis-and-evaluation-of-4-aminoquinoline-purine-hybrids-as-potential-antiplasmodial-agents
#16
P Linga Reddy, Shabana I Khan, Prija Ponnan, Mohit Tripathi, Diwan S Rawat
A novel series of 4-aminoquinoline-purine hybrids were synthesized and assessed for their antiplasmodial activity against CQ-sensitive and CQ-resistant strains of P. falciparum. It was envisaged that linking of the 4-aminoquinoline pharmacophore (targeting heme-detoxification pathway of malarial parasite) with the purine functionality (targeting plasmodial HG(X)PRT enzyme) will produce a hybrid antiplasmodial agent with increased potency. The synthesized hybrids displayed good antiplasmodial activities against both the sensitive and resistant strains of P...
December 1, 2016: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/27936444/amino-derivatives-of-platanic-acid-act-as-selective-and-potent-inhibitors-of-butyrylcholinesterase
#17
Lucie Heller, Michael Kahnt, Anne Loesche, Patricia Grabandt, Stefan Schwarz, Wolfgang Brandt, René Csuk
A set of thirtyfive 30-norlupan derivatives (2-36) was prepared from the natural triterpenoid platanic acid (PA), and the hydroxyl group at C-3, the carboxyl group at C-17 and the carbonyl group at C-20 were modified. These derivatives were tested for their inhibitory activity for the enzymes acetylcholinesterase (AChE, from electric eel) and butyrylcholinesterase (BChE, from equine serum) using Ellman's assay. Extra enzyme kinetic studies were performed. The most active compound was (3β, 20R)-3-acetyloxy-20-amino-30-norlupan-28-oate (32) showing a Ki value of 0...
November 30, 2016: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/27936440/stride-variability-measures-derived-from-wrist-and-hip-worn-accelerometers
#18
Jacek K Urbanek, Jaroslaw Harezlak, Nancy W Glynn, Tamara Harris, Ciprian Crainiceanu, Vadim Zipunnikov
Many epidemiological and clinical studies use accelerometry to objectively measure physical activity using the activity counts, vector magnitude, or number of steps. These measures use just a fraction of the information in the raw accelerometry data as they are typically summarized at the minute level. To address this problem, we define and estimate two measures of temporal stride-to-stride gait variability based on raw accelerometry data: Amplitude Deviation (AD) and Phase Deviation (PD). We explore the sensitivity of our approach to on-body placement of the accelerometer by comparing hip, left and right wrist placements...
November 30, 2016: Gait & Posture
https://www.readbyqxmd.com/read/27936436/spectroscopic-single-crystal-x-ray-hirshfeld-in-vitro-and-in-silico-biological-evaluation-of-a-new-series-of-potent-thiazole-nucleus-integrated-with-pyrazoline-scaffolds
#19
Vinutha V Salian, Badiadka Narayana, Balladka K Sarojini, Madan S Kumar, Govinahalli S Nagananda, Kullaiah Byrappa, Avinash K Kudva
In the present study, the spectroscopic characterization of a new series of substituted thiazole linked pyrazoline scaffolds 4a-l was performed. The formation of 4a-l from the intermediate 3-(4-chlorophenyl)-5-[4-(propan-2-yl)phenyl]-4,5-dihydro-1H-pyrazole-1-carbothioamide 2 and substituted 2-bromo-1-phenylethanone 3a-l was evidenced through the changes in FTIR, (1)H NMR, (13)C NMR, LCMS data. The X-ray diffraction studies revealed that compound 2 and 4g crystallized in monoclinic crystal system with P21/n space group...
November 28, 2016: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/27936434/mineralocorticoid-receptor-haplotype-estradiol-progesterone-and-emotional-information-processing
#20
Danielle A Hamstra, E Ronald de Kloet, Ina Quataert, Myrthe Jansen, Willem Van der Does
BACKGROUND: Carriers of MR-haplotype 1 and 3 (GA/CG; rs5522 and rs2070951) are more sensitive to the influence of oral contraceptives (OC) and menstrual cycle phase on emotional information processing than MR-haplotype 2 (CA) carriers. We investigated whether this effect is associated with estradiol (E2) and/or progesterone (P4) levels. METHOD: Healthy MR-genotyped premenopausal women were tested twice in a counterbalanced design. Naturally cycling (NC) women were tested in the early-follicular and mid-luteal phase and OC-users during OC-intake and in the pill-free week...
November 30, 2016: Psychoneuroendocrinology
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