keyword
MENU ▼
Read by QxMD icon Read
search

intestinal mucosal barrier

keyword
https://www.readbyqxmd.com/read/28441885/lactobacillus-plantarum-l9-but-not-lactobacillus-acidophilus-la-reduces-tumour-necrosis-factor-induced-bacterial-translocation-in-caco-2-cells
#1
B Wang, J Chen, S Wang, X Zhao, G Lu, X Tang
Translocation of bacteria across the intestinal barrier is important in the pathogenesis of systemic sepsis and multiple organ dysfunction syndromes. Inflammatory cytokines increase paracellular permeability that allows increased luminal bacteria to translocate across mucosal epithelium and further deteriorate the gut barrier. In order to reduce this risk, the prophylactic use of probiotics has been recently addressed. In this paper, we investigate the protective role toward tumour necrosis factor (TNF)-α induced non-pathogenic Escherichia coli translocation across Caco-2 monolayers of Lactobacillus strains...
April 26, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/28440501/transfer-of-cd11c-lamina-propria-mononuclear-phagocytes-from-post-infectious-irritable-bowel-syndrome-causes-mucosal-barrier-dysfunction-and-visceral-hypersensitivity-in-recipient-mice
#2
Ya-Jun Ren, Lei Zhang, Tao Bai, Hong-Lu Yu, Ying Li, Wei Qian, Si Jin, Zhi-Fan Xiong, Huan Wang, Xiao-Hua Hou
The role of low-grade inflammation in the development of post‑infectious irritable bowel syndrome (PI‑IBS) has attracted increasing attention. Abnormal CD11c+ mononuclear phagocytes, such as dendritic cells (DCs), macrophages, and monocytes, are involved in the disruption of immune tolerance in organisms, which can lead to the development of chronic inflammatory diseases. The present study tested the hypothesis that CD11c+ lamina propria mononuclear phagocytes (CD11c+ LPMPs) contribute to increased mucosal permeability and visceral hypersensitivity in a PI‑IBS mouse model...
April 25, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28436589/short-chain-fatty-acids-augment-rat-duodenal-mucosal-barrier-function
#3
Wan Salman Wan Saudi, Markus Sjöblom
Short-chain fatty acids (SCFAs) are produced by bacterial fermentation in the large intestine, particularly from diets containing fibres and carbohydrates. The small intestinal epithelium is exposed to SCFAs derived mainly from oral bacteria or food supplementation. Although luminal nutrients are important in regulation of intestinal functions the role of SCFAs in regulation of small intestinal mucosal barrier function and motility has not been fully described. The aim of the present study was to elucidate the effects of acetate and propionate on duodenal mucosal barrier function and motility...
April 24, 2017: Experimental Physiology
https://www.readbyqxmd.com/read/28432022/a-crosstalk-between-muscarinic-and-crf2-receptors-regulates-cellular-adhesion-properties-of-human-colon-cancer-cells
#4
M Pelissier-Rota, N T Chartier, B Bonaz, M R Jacquier-Sarlin
Patients with inflammatory bowel disease often suffer from chronic and relapsing intestinal inflammation that favor the development of colitis associated cancer. An alteration of the epithelial intestinal barrier function observed in IBD is supposed to be a consequence of stress. It has been proposed that corticotrophin-releasing factor receptor (CRF2), one of the two receptors of CRF, the principal neuromediator of stress, acts on cholinergic nerves to induce stress-mediated epithelial barrier dysfunction...
April 18, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28428961/glucose-insulin-potassium-alleviates-intestinal-mucosal-barrier-injuries-involving-decreased-expression-of-uncoupling-protein-2-and-nlr-family-pyrin-domain-containing-3-inflammasome-in-polymicrobial-sepsis
#5
Jun-Liang Zhang, Yi-Ting Chen, Guang-Dao Chen, Tao Wang, Ju-Xin Zhang, Qi-Yi Zeng
Uncoupling protein 2 (UCP2) may be critical for intestinal barrier function which may play a key role in the development of sepsis, and insulin has been reported to have anti-inflammatory effects. Male Sprague-Dawley rats were randomly allocated into five groups: control group, cecal ligation and puncture (CLP) group, sham surgery group, CLP plus glucose-insulin-potassium (GIK) group, and CLP plus glucose and potassium (GK) group. Ileum tissues were collected at 24 h after surgery. Histological and cytokine analyses, intestinal permeability tests, and western blots of intestinal epithelial tight junction component proteins and UCP2 were performed...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28425012/a-study-of-the-damage-of-the-intestinal-mucosa-barrier-structure-and-function-of-ctenopharyngodon-idella-with-aeromonas-hydrophila
#6
Wei-Guang Kong, Si-Si Li, Xiao-Xuan Chen, Yu-Qing Huang, Ying Tang, Zhi-Xin Wu
The aim of this study is to explore the effect of Aeromonas hydrophila on the intestinal mucosal barrier structure and intestinal permeability in grass carp (Ctenopharyngodon idella). Histopathological examinations showed that A. hydrophila induced severe intestinal lesions, including inflammatory cell infiltration and intestinal villus fusion and swelling. Messenger RNA (mRNA) expression of the inflammatory cytokines TNF-α, IL-1β, IL-8, IL-10 and MyD88 was significantly increased after infection with A. hydrophila...
April 19, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28417991/esophageal-and-small-intestinal-mucosal-integrity-in-eosinophilic-esophagitis-and-response-to-an-elemental-diet
#7
Marijn J Warners, Berber J Vlieg-Boerstra, Joanne Verheij, Patricia H P van Hamersveld, Bram D van Rhijn, Marleen T J Van Ampting, Lucien F Harthoorn, Wouter J de Jonge, Andreas J P M Smout, Albert J Bredenoord
OBJECTIVES: The esophageal mucosal integrity is impaired in eosinophilic esophagitis (EoE) and it has been suggested that the duodenal permeability is increased. The absence of food allergens may restore the integrity. The aims of this study were to assess duodenal permeability in EoE and to evaluate the effect of an elemental diet on the esophageal and duodenal integrity. METHODS: In this prospective study 17 adult EoE patients and 8 healthy controls (HC) were included...
April 18, 2017: American Journal of Gastroenterology
https://www.readbyqxmd.com/read/28413062/assessment-of-mucosal-integrity-by-quantifying-neutrophil-granulocyte-influx-in-murine-models-of-acute-intestinal-injury
#8
Julius Clemens Fischer, Alexander Wintges, Tobias Haas, Hendrik Poeck
Intact epithelial body surfaces represent physical barriers which protect the organism from invading pathogens and loss of nutrients. Barrier malfunction is closely linked to disorders such as inflammatory bowel disease and graft-versus-host disease. In fact, several pharmacological or radiobiological therapeutic strategies have side effects that affect epithelial surfaces. In this context, assays that accurately assess epithelial barrier integrity in patients and animal models are crucial to create a better understanding of the mechanisms leading to disease or limiting therapeutic approaches due to barrier disruption...
April 11, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28413046/heat-stress-decreases-expression-of-the-cytokines-avian-%C3%AE-defensins-4-and-6-and-toll-like-receptor-2-in-broiler-chickens-infected-with-salmonella-enteritidis
#9
W M Quinteiro-Filho, A S Calefi, D S G Cruz, T P A Aloia, A Zager, C S Astolfi-Ferreira, J A Piantino Ferreira, S Sharif, J Palermo-Neto
A high ambient temperature is a highly relevant stressor in poultry production. Heat stress (HS) has been reported to reduce animal welfare, performance indices and increase Salmonella susceptibility. Salmonella spp. are major zoonotic pathogen that cause over 1 billion of human infections worldwide annually. Therefore, the current study was designed to analyze the effect of heat stress on Salmonella infection in chickens through modulation of the immune responses. Salmonella Enteritidis was inoculated via gavage at one day of age (10(6)cfu/mL)...
April 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28406732/black-adzuki-bean-vigna-angularis-attenuates-high-fat-diet-induced-colon-inflammation-in-mice
#10
Jin-Seon Yook, Kyung-Ah Kim, Mina Kim, Youn-Soo Cha
Adzuki beans (Vigna angularis), one of the most important legume crops in East Asia, have been shown to possess potential antioxidant and anti-inflammatory effects in mice. Here, we investigated the effects of black adzuki bean (BAB) on colon inflammation triggered by high-fat diet (HD)-induced obesity in mice. We also isolated lipopolysaccharide (LPS)-stimulated peritoneal macrophages and assessed inflammation-related parameters. We found that BAB decreased the concentrations of LPS and various circulating proinflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 in mice with HD-induced obesity...
April 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/28405139/regulation-of-intestinal-permeability-the-role-of-proteases
#11
REVIEW
Hanne Van Spaendonk, Hannah Ceuleers, Leonie Witters, Eveline Patteet, Jurgen Joossens, Koen Augustyns, Anne-Marie Lambeir, Ingrid De Meester, Joris G De Man, Benedicte Y De Winter
The gastrointestinal barrier is - with approximately 400 m(2) - the human body's largest surface separating the external environment from the internal milieu. This barrier serves a dual function: permitting the absorption of nutrients, water and electrolytes on the one hand, while limiting host contact with noxious luminal antigens on the other hand. To maintain this selective barrier, junction protein complexes seal the intercellular space between adjacent epithelial cells and regulate the paracellular transport...
March 28, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28400412/western-diet-consumption-induces-alteration-of-barrier-function-mechanisms-in-the-ileum-that-correlates-with-metabolic-endotoxemia-in-rats
#12
Mathilde Guerville, Anaïs Leroy, Annaëlle Sinquin, Fabienne Laugerette, Marie-Caroline Michalski, Gaëlle Boudry
Obesity and its related disorders have been associated to the presence in the blood of gut bacteria-derived lipopolysaccharides (LPS). However, the factors underlying this low-grade elevation in plasma LPS, so-called metabolic endotoxemia, are not fully elucidated. We aimed to investigate the effects of Western diet (WD) feeding on intestinal and hepatic LPS handling mechanisms in a rat model of diet-induced obesity (DIO). Rats were fed either a standard chow diet (C) or a Western Diet (WD, 45% fat) for 6 weeks...
April 11, 2017: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28395430/-changes-of-adherens-junctions-between-the-epithelial-cells-of-intestinal-mucosal-barrier-in-irritable-bowel-syndrome
#13
C Y Chen, M Zhang, S S Wang, B Lü, X Wang
Objective: To investigate whether there are changes in adherens junctions(AJ), including E-cadherin (E-cad) and β-catenin (β-cat), in the colonic mucosa of irritable bowel syndrome (IBS). Methods: Colorectal dilatation combined with restraint stress was used to establish IBS SD rat model. There were 8 rats in the model group and 8 rats in normal control group. From each rat, the colon tissue 5 centimeters away from ileocecum (I) and the junction of the rectum and sigmoid colon (R) were taken, in which E-cad and β-cat mRNA, protein level and structural changes were detected using real-time quantitative-PCR, Western blot, and immunofluorescence...
April 11, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28384243/neuropeptide-s-reduces-duodenal-bicarbonate-secretion-and-ethanol-induced-increases-in-duodenal-motility-in-rats
#14
Wan Salman Wan Saudi, Markus Sjöblom
Alcohol disrupts the intestinal mucosal barrier by inducing metabolic and functional changes in epithelial cells. Recently, we showed that neuropeptide S (NPS) decreases duodenal motility and increases mucosal paracellular permeability, suggesting a role of NPS in the pathogenesis of disorders and dysfunctions in the small intestine. The aim of the present study was to investigate the effects of NPS on ethanol- and HCl-induced changes of duodenal mucosal barrier function and motility. Rats were anaesthetized with thiobarbiturate, and a 30-mm segment of the proximal duodenum with an intact blood supply was perfused in situ...
2017: PloS One
https://www.readbyqxmd.com/read/28374966/apoptosis-dysbiosis-and-expression-of-inflammatory-cytokines-are-sequential-events-in-the-development-of-5-fluorouracil-induced-intestinal-mucositis-in-mice
#15
Nahla Hamouda, Tatsushi Sano, Yosuke Oikawa, Toru Ozaki, Masaki Shimakawa, Kenjiro Matsumoto, Kikuko Amagase, Kazuhide Higuchi, Shinichi Kato
The chemotherapeutic agent 5-fluorouracil (5-FU) causes intestinal mucositis with severe diarrhoea but the pathogenesis is not fully understood. In the present study, we investigated the pathogenic effects of 5-FU in mice, focusing on apoptosis, enterobacteria and inflammatory cytokines. Repeated administration of 5-FU caused severe intestinal mucositis on day 6, accompanied by diarrhoea and body weight loss. TNF-α expression increased one day after exposure to the drug, and spiked a second time on day 4, at which point myeloperoxidase activity and IL-1β expression also increased...
April 4, 2017: Basic & Clinical Pharmacology & Toxicology
https://www.readbyqxmd.com/read/28373746/cyanidin-3-o-glucoside-modulates-the-in-vitro-inflammatory-crosstalk-between-intestinal-epithelial-and-endothelial-cells
#16
Daniela Ferrari, Francesco Cimino, Deborah Fratantonio, Maria Sofia Molonia, Romina Bashllari, Rossana Busà, Antonella Saija, Antonio Speciale
Intestinal epithelium represents a protective physical barrier and actively contributes to the mucosal immune system. Polarized basolateral intestinal secretion of inflammatory mediators, followed by activation of NF-κB signaling and inflammatory pathways in endothelial cells, efficiently triggers extravasation of neutrophils from the vasculature, therefore contributing to the development and maintenance of intestinal inflammation. Proper regulation of NF-κB activation at the epithelial interface is crucial for the maintenance of physiological tissue homeostasis...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/28368012/illuminating-dynamic-neutrophil-trans-epithelial-migration-with-micro-optical-coherence-tomography
#17
Kengyeh K Chu, Mark E Kusek, Linbo Liu, Avira Som, Lael M Yonker, Huimin Leung, Dongyao Cui, Jinhyeob Ryu, Alexander D Eaton, Guillermo J Tearney, Bryan P Hurley
A model of neutrophil migration across epithelia is desirable to interrogate the underlying mechanisms of neutrophilic breach of mucosal barriers. A co-culture system consisting of a polarized mucosal epithelium and human neutrophils can provide a versatile model of trans-epithelial migration in vitro, but observations are typically limited to quantification of migrated neutrophils by myeloperoxidase correlation, a destructive assay that precludes direct longitudinal study. Our laboratory has recently developed a new isotropic 1-μm resolution optical imaging technique termed micro-optical coherence tomography (μOCT) that enables 4D (x,y,z,t) visualization of neutrophils in the co-culture environment...
April 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28366996/claudin-2-expression-is-upregulated-in-the-ileum-of-diarrhea-predominant-irritable-bowel-syndrome-patients
#18
Haruka Ishimoto, Tadayuki Oshima, Hiroo Sei, Takahisa Yamasaki, Takashi Kondo, Katsuyuki Tozawa, Toshihiko Tomita, Yoshio Ohda, Hirokazu Fukui, Jiro Watari, Hiroto Miwa
Intestinal epithelial barrier function is impaired in irritable bowel syndrome patients. Claudins are highly expressed in cells with tight junctions and are involved in the intestinal epithelial barrier function. The expression pattern of tight junction proteins in diarrhea-predominant irritable bowel syndrome have not been fully elucidated. We therefore recruited 17 diarrhea-predominant irritable bowel syndrome patients and 20 healthy controls. The expression of the tight junction-related proteins was examined in the ileal, cecal, and rectal mucosa of diarrhea-predominant irritable bowel syndrome patients using real-time PCR and immunofluorescence...
March 2017: Journal of Clinical Biochemistry and Nutrition
https://www.readbyqxmd.com/read/28359333/the-immune-regulating-effect-of-xiao-er-qixingcha-in-constipated-mice-induced-by-high-heat-and-high-protein-diet
#19
Chang Qu, Guang-Hua Yang, Rong-Bo Zheng, Xiu-Ting Yu, Shao-Zhong Peng, Jian-Hui Xie, Jian-Nan Chen, Xiu-Fen Wang, Zi-Ren Su, Xiao-Jun Zhang
BACKGROUND: Xiao'er Qixingcha (EXQ) has been extensively applied to relieve dyspepsia and constipation in children for hundreds of years in China. However, the therapeutic mechanism underlying its efficacy remained to be defined. The present study aimed to clarify the possible laxative and immune-regulating effects of EXQ on two models of experimental constipation in mice, which mimicked the pediatric constipation caused by high-heat and high-protein diet (HHPD). METHODS: The two models of constipated mice were induced by HHPD or HHPD + atropine respectively...
March 31, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28357579/epithelial-cell-types-and-their-proposed-roles-in-maintaining-the-mucosal-barrier-in-human-chagasic-megacolonic-mucosa
#20
Christian Koch, Alexandre B M da Silveira, Enio C de Oliveira, Karl Quint, Winfried Neuhuber, Axel Brehmer, Samir Jabari
Patients suffering from chagasic megacolon must have an intact mucosal barrier as they survive this chronic disease for decades. A key structure of the mucosal barrier are epithelial cells. Vasoactive-intestinal-peptide (VIP)-positive nerve fibres are involved in influencing, e.g., epithelial cell proliferation, mucus secretion (e.g., mucin 2 and trefoil factor 3 of goblet cells) and inflammation or autoimmunity, all putative and/or known factors altered in chagasic megacolon. We analyzed qualitatively and quantitatively goblet cells, their specific markers, such as mucin 2 (MUC2) and trefoil factor 3 (TFF3) and enterocytes, the relation of VIP-immunoreactive nerve fibres to the epithelia, the distribution of gelsolin, a protein involved in chronic inflammation processes in the epithelia, and the proliferation rate of epithelial cells by combined 4',6-diamidino-2-phenylindole (DAPI) and phosphohistone-H3 (PHH3) staining...
March 29, 2017: Histochemistry and Cell Biology
keyword
keyword
84660
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"