Read by QxMD icon Read

delayed sequence induction

Yael Yoffe, Maya David, Rinat Kalaora, Lital Povodovski, Gilgi Friedlander, Ester Feldmesser, Elena Ainbinder, Ann Saada, Shani Bialik, Adi Kimchi
Multiple transcriptional and epigenetic changes drive differentiation of embryonic stem cells (ESCs). This study unveils an additional level of gene expression regulation involving noncanonical, cap-independent translation of a select group of mRNAs. This is driven by death-associated protein 5 (DAP5/eIF4G2/NAT1), a translation initiation factor mediating IRES-dependent translation. We found that the DAP5 knockdown from human ESCs (hESCs) resulted in persistence of pluripotent gene expression, delayed induction of differentiation-associated genes in different cell lineages, and defective embryoid body formation...
September 1, 2016: Genes & Development
I Alexandra Amaro, Lee A Henderson
Huntington's disease (HD) is a fatal progressive disease linked to expansion of glutamine repeats in the huntingtin protein and characterized by the progressive loss of cognitive and motor function. We show that expression of a mutant human huntingtin exon-1-GFP fusion construct results in nonspecific gene dysregulation that is significantly reduced by 50% due to coexpression of INT41, an intrabody specific for the proline-rich region of the huntingtin protein. Using stable PC12 cell lines expressing either inducible human mutant huntingtin (mHtt, Q73) or normal huntingtin (nHtt, Q23), we investigated the effect of rAAV6-INT41, an adeno-associated virus vector with the INT41 coding sequence, on the subcellular distribution of Htt...
2016: Journal of Neurodegenerative Diseases
Yunseon Choi, Sang Taek Oh, Min-Ah Won, Kyung Mi Choi, Min Ji Ko, Daekwan Seo, Tae-Won Jeon, In Hye Baik, Sang-Kyu Ye, Keon Uk Park, In-Chul Park, Byeong-Churl Jang, Jun-Young Seo, Yun-Han Lee
Ornithine decarboxylase 1 (ODC1), a metabolic enzyme critically involved in the polyamine biosynthesis, is commonly upregulated in hepatocellular carcinoma (HCC). Despite its altered expression in human HCC tissues, the molecular mechanism by which ODC1 alters the course of HCC progression and functions in HCC cell survival is unknown. Here we identified that silencing of ODC1 expression with small interfering (si) RNA causes inhibition of HCC cell growth through blockade of cell cycle progression and induction of apoptosis...
September 30, 2016: Biochemical and Biophysical Research Communications
Mingyue Lv, Amr Ahmed Mohamed, Liwei Zhang, Pengfei Zhang, Long Zhang
Insect defensins are effector components of the innate defense system. During infection, these peptides may play a role in the control of pathogens by providing protective antimicrobial barriers between epithelial cells and the hemocoel. The cDNAs encoding four defensins of the migratory locust, Locusta migratoria, designated LmDEF 1, 3-5, were identified for the first time by transcriptome-targeted analysis. Three of the members of this CSαβ defensin family, LmDEF 1, 3, and 5, were detected in locust tissues...
2016: PloS One
Wenqi Li, Xiaohua Liu, Yingmin Lu
BACKGROUND: Oriental hybrid lily 'Sorbonne', a very important cut flower for lily, is enjoyed great popularity in the world, but it must experience a period of low winter temperature to initiate or accelerate the flowering process. To gain a better understanding of the temperature signaling pathway and the molecular metabolic reactions involved in the vernalization response, a genome-wide transcriptional analysis using RNA-Seq was performed. RESULTS: 188,447,956 sequencing reads was assembled into 66,327 unigenes and showed similarity to known proteins in the Swiss-Prot protein database, and 2,893, 30,406 and 60,737 unigenes aligned to existing sequences in the KEGG, COG, and GO databases...
2016: BMC Genomics
Jinwoo Lee, Tiegang Tong, Haichuan Duan, Yee Hoon Foong, Ibrahim Musaitif, Takeshi Yamazaki, Colin Jefcoate
The cholesterol transfer function of steroidogenic acute regulatory protein (StAR) is uniquely integrated into adrenal cells, with mRNA translation and protein kinase A (PKA) phosphorylation occurring at the mitochondrial outer membrane (OMM). The StAR C-terminal cholesterol-binding domain (CBD) initiates mitochondrial intermembrane contacts to rapidly direct cholesterol to Cyp11a1 in the inner membrane (IMM). The conserved StAR N-terminal regulatory domain (NTD) includes a leader sequence targeting the CBD to OMM complexes that initiate cholesterol transfer...
2016: Frontiers in Endocrinology
Joanna Halliwell, Philippa Borrill, Anna Gordon, Radoslaw Kowalczyk, Marina L Pagano, Benedetta Saccomanno, Alison R Bentley, Cristobal Uauy, James Cockram
To date, a small number of major flowering time loci have been identified in the related Triticeae crops, bread wheat (Triticum aestivum), durum wheat (T. durum), and barley (Hordeum vulgare). Natural genetic variants at these loci result in major phenotypic changes which have adapted crops to the novel environments encountered during the spread of agriculture. The polyploid nature of bread and durum wheat means that major flowering time loci in which recessive alleles confer adaptive advantage in related diploid species have not been readily identified...
2016: Frontiers in Plant Science
X-X Xu, Y-Q Zhang, S Freed, J Yu, Y-F Gao, S Wang, L-N Ouyang, W-Y Ju, F-L Jin
Insect defensins, are cationic peptides that play an important role in immunity against microbial infection. In the present study, an anionic defensin from Plutella xylostella, (designated as PxDef) was first cloned and characterized. Amino acid sequence analysis showed that the mature peptide owned characteristic six-cysteine motifs with predicted isoelectric point of 5.57, indicating an anionic defensin. Quantitative real-time polymerase chain reaction analysis showed that PxDef was significantly induced in epidermis, fat body, midgut and hemocytes after injection of heat-inactivated Bacillus thuringiensis, while such an induction was delayed by the injection of live B...
July 22, 2016: Bulletin of Entomological Research
Markus Rienth, Laurent Torregrosa, Gautier Sarah, Morgane Ardisson, Jean-Marc Brillouet, Charles Romieu
BACKGROUND: Fruit composition at harvest is strongly dependent on the temperature during the grapevine developmental cycle. This raises serious concerns regarding the sustainability of viticulture and the socio-economic repercussions of global warming for many regions where the most heat-tolerant varieties are already cultivated. Despite recent progress, the direct and indirect effects of temperature on fruit development are far from being understood. Experimental limitations such as fluctuating environmental conditions, intra-cluster heterogeneity and the annual reproductive cycle introduce unquantifiable biases for gene expression and physiological studies with grapevine...
2016: BMC Plant Biology
Robert B Gerbing, Todd A Alonzo, Lillian Sung, Alan S Gamis, Soheil Meshinchi, Sharon E Plon, Alison A Bertuch, Maria M Gramatges
PURPOSE: Suboptimal outcomes for children with acute myeloid leukemia (AML) necessitate maximally intensive therapy. Consequently, serious adverse events, such as prolonged periods of profound myelosuppression, contribute to AML treatment-related mortality. Telomeres, the repetitive DNA-protein structures at chromosome ends, influence cellular replicative capacity in that critically short telomeres can induce cell senescence or apoptosis. Our objective was to evaluate the impact of telomere length on duration of post-therapy neutropenia in a pediatric AML cohort...
June 27, 2016: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
R Vidya, M Makesh, C S Purushothaman, A Chaudhari, P Gireesh-Babu, K V Rajendran
Leucine-rich repeat (LRR) proteins are present in all living organisms, and their participation in signal transduction and defense mechanisms has been elucidated in humans and mosquitoes. LRRs possibly involve in protein-protein interactions also and show differential expression pattern upon challenge with pathogens. In the present study, a new LRR gene was identified in mud crab, Scylla serrata. LRR gene mRNA levels in different developmental stages and various tissues of S. serrata were analysed. Further, the response of the gene against different ligands, Gram-negative bacterium, and white spot syndrome virus (WSSV) was investigated in vitro and in vivo...
September 15, 2016: Gene
Susan Tsai, Douglas B Evans
IMPORTANCE: It is estimated that pancreatic cancer (PC) will become the second leading cause of cancer-related death in the United States by 2030. OBSERVATIONS: Clinical and preclinical data support the understanding that PC metastases occur early in the pathogenesis of this disease, even before the primary tumor can be detected. This has important implications for the clinical management of patients with localized PC, as surgery alone is unlikely to be curative for most patients...
September 1, 2016: JAMA Surgery
Vinayak S Rohan, David J Taber, Omar Moussa, Nicole A Pilch, Signe Denmark, Holly B Meadows, John W McGillicuddy, Kenneth D Chavin, Prabhakar K Baliga, Charles F Bratton
OBJECIVES: Elevated panel reactive antibody levels have been traditionally associated with increased acute rejection rate and decreased long-term graft survival after kidney transplant. In this study, our objective was to determine patient and allograft outcomes in sensitized kidney transplant recipients with advanced HLA antibody detection and stringent protein sequence epitope analyses. MATERIALS AND METHODS: This was a subanalysis of a prospective, risk-stratified randomized controlled trial that compared interleukin 2 receptor antagonist to rabbit antithymocyte globulin induction in 200 kidney transplant recipients, examining outcomes based on panel reactive antibody levels of < 20% (low) versus ≥ 20% (high, sensitized)...
June 3, 2016: Experimental and Clinical Transplantation
Alberto M Gochez, Deepak Shantharaj, Neha Potnis, Xiaofeng Zhou, Gerald V Minsavage, Frank F White, Nian Wang, Jason C Hurlbert, Jeffrey B Jones
Xanthomonas fuscans subsp. aurantifolii group C strains exhibit host specificity on different citrus species. The strains possess a type III effector, AvrGf2, belonging to the XopAG effector gene family, which restricts host range on citrus. We dissected the modular nature and mode of action of AvrGf2 in grapefruit resistance. XopAG effectors possess characteristic features, such as a chloroplast localization signal, a cyclophilin binding domain characteristic amino acid sequence motif (GPLL), and a C-terminal domain containing CLNAxYD...
March 31, 2016: Molecular Plant Pathology
Iaroslava Kos, Miranda J Patterson, Sadri Znaidi, Despoina Kaloriti, Alessandra da Silva Dantas, Carmen M Herrero-de-Dios, Christophe d'Enfert, Alistair J P Brown, Janet Quinn
UNLABELLED: Following phagocytosis, microbes are exposed to an array of antimicrobial weapons that include reactive oxygen species (ROS) and cationic fluxes. This is significant as combinations of oxidative and cationic stresses are much more potent than the corresponding single stresses, triggering the synergistic killing of the fungal pathogenCandida albicansby "stress pathway interference." Previously we demonstrated that combinatorial oxidative plus cationic stress triggers a dramatic increase in intracellular ROS levels compared to oxidative stress alone...
2016: MBio
Estanislao Nistal-Villan, Joanna Poutou, Estefania Rodríguez-Garcia, Maria Buñuales, Beatriz Carte-Abad, Jesus Prieto, Gloria Gonzalez-Aseguinolaza, Ruben Hernandez-Alcoceba, Esther Larrea
Development of reporter systems for in vivo examination of IFN-β induction or signaling of type I interferon (IFN-I) pathways is of great interest in order to characterize biological responses to different inducers such as viral infections. Several reporter mice have been developed to monitor the induction of both pathways in response to different agonists. However, alternative strategies that do not require transgenic mice breeding have to date not been reported. In addition, detection of these pathways in vivo in animal species other than mice has not yet been addressed...
2016: PloS One
Cristina Pinedo, Javier Moraga, Javier Barua, Victoria E González-Rodríguez, Josefina Aleu, Rosa Durán-Patrón, Antonio J Macías-Sánchez, James R Hanson, Muriel Viaud, Rosario Hernández-Galán, Carlos Garrido, Isidro G Collado
The sequencing of the genomes of the B05.10 and T4 strains of the fungus Botrytis cinerea revealed an abundance of novel biosynthetic gene clusters, the majority of which were unexpected on the basis of the previous analyses of the fermentation of these and closely related species. By systematic alteration of easy accessible cultivation parameters, using chemical induction with copper sulfate, we have found a cryptic sesquiterpenoid family with new structures related to eremophil-9-ene, which had the basic structure of the sesquiterpene (+)-5-epiaristolochene ((+)-4-epieremophil-9-ene)...
May 20, 2016: ACS Chemical Biology
Sara Marie Atkinson, Janine Bleil, René Maier, Anja A Kühl, Mette Thorn, Kyle Serikawa, Brian Fox, Kim Kruse, Claus Haase, Søren Skov, Anneline Nansen, Uta Syrbe
BACKGROUND: The aims of the present study were to determine the relationship between bone destruction and bone formation in the delayed-type hypersensitivity arthritis (DTHA) model and to evaluate the effect of receptor activator of nuclear factor κB ligand (RANKL) blockade on severity of arthritis, bone destruction, and bone formation. METHODS: DTHA was induced in C57BL/6 mice. Inflammation, erosive joint damage, and new bone formation were semiquantitatively scored by histology...
2016: Arthritis Research & Therapy
Matthew B Veldman, Yesenia Rios-Galdamez, Xiao-Hong Lu, Xiaofeng Gu, Wei Qin, Song Li, X William Yang, Shuo Lin
BACKGROUND: Although the genetic cause for Huntington's disease (HD) has been known for over 20 years, the mechanisms that cause the neurotoxicity and behavioral symptoms of this disease are not well understood. One hypothesis is that N-terminal fragments of the HTT protein are the causative agents in HD and that peptide sequences adjacent to the poly-glutamine (Q) repeats modify its toxicity. Here we test the function of the N-terminal 17 amino acids (N17) in the context of the exon 1 fragment of HTT in a novel, inducible zebrafish model of HD...
2015: Molecular Neurodegeneration
Melanie Rall, Daniela Kraft, Meta Volcic, Aljona Cucu, Elena Nasonova, Gisela Taucher-Scholz, Halvard Bönig, Lisa Wiesmüller, Claudia Fournier
Ionizing radiation generates DNA double-strand breaks (DSB) which, unless faithfully repaired, can generate chromosomal rearrangements in hematopoietic stem and/or progenitor cells (HSPC), potentially priming the cells towards a leukemic phenotype. Using an enhanced green fluorescent protein (EGFP)-based reporter system, we recently identified differences in the removal of enzyme-mediated DSB in human HSPC versus mature peripheral blood lymphocytes (PBL), particularly regarding homologous DSB repair (HR). Assessment of chromosomal breaks via premature chromosome condensation or γH2AX foci indicated similar efficiency and kinetics of radiation-induced DSB formation and rejoining in PBL and HSPC...
2015: Frontiers in Oncology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"