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Eahy926 cell line

Tarek Saleh, Ebtehal Ahmed, Lina Yu, Kamal Hussein, Kyung-Mee Park, Yun-Suk Lee, Byung-Jae Kang, Ki-Young Choi, Sooyoung Choi, Kyung-Sun Kang, Heung-Myong Woo
No ideal cross-linking agent has been identified for decellularized livers (DLs) yet. In this study, we evaluated structural improvements and biocompatibility of porcine DLs after cross-linking with silver nanoparticles (AgNPs). Porcine liver slices were decellularized and then loaded with AgNPs (100 nm) after optimization of the highest non-toxic concentration (5 µg/mL) using Human hepatocellular carcinoma (HepG2) and EAhy926 human endothelial cell lines. The cross-linking effect of AgNPs was evaluated and compared to that of glutaraldehyde and ethyl carbodiimide hydrochloride and N-hydroxysuccinimide...
March 27, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Jaganathan Kowshik, Rajakishore Mishra, Josephraj Sophia, Satabdi Rautray, Kumaraswamy Anbarasu, G Deepak Reddy, Madhulika Dixit, Sundarasamy Mahalingam, Siddavaram Nagini
Reversion-inducing cysteine-rich protein with Kazal motifs (RECK), a potent inhibitor of matrix metalloproteinases (MMPs) is a common negative target of oncogenic signals and a potential therapeutic target for novel drug development. Here, we show that sequential RECKlessness stimulates angiogenesis and Notch signalling in the 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis model, a paradigm for oral oncogenesis and chemointervention. We also report the chemotherapeutic effect of nimbolide, a limonoid from the neem tree (Azadirachta indica) based on the upregulation of RECK as well as modulation of the expression of key molecules involved in invasion and angiogenesis...
May 17, 2017: Scientific Reports
Reatul Karim, Claudio Palazzo, Julie Laloy, Anne-Sophie Delvigne, Stéphanie Vanslambrouck, Christine Jerome, Elise Lepeltier, Francois Orange, Jean-Michel Dogne, Brigitte Evrard, Catherine Passirani, Géraldine Piel
The purpose of this study was to develop different injectable nanosized drug delivery systems (NDDSs) i.e. liposome, lipid nanocapsule (LNC) and polymeric nanocapsule (PNC) encapsulating apigenin (AG) and compare their characteristics to identify the nanovector(s) that can deliver the largest quantity of AG while being biocompatible. Two liposomes with different surface characteristics (cationic and anionic), a LNC and a PNC were prepared. A novel tocopherol modified poly(ethylene glycol)-b-polyphosphate block-copolymer was used for the first time for the PNC preparation...
November 5, 2017: International Journal of Pharmaceutics
Misae Tsunaka, Haruka Shinki, Takatoshi Koyama
Idarubicin (IDR), cytarabine (AraC), and tamibarotene (Am80) are effective for treatment of acute myeloid leukemia (AML). In acute leukemia, the incidence of venous thromboembolism or disseminated intravascular coagulation is associated with induction chemotherapy. Procoagulant effects of IDR, AraC, and Am80 were investigated in a vascular endothelial cell line EAhy926 and AML cell lines HL60 (AML M2), NB4 (AML M3, APL), and U937 (AML M5), focusing on tissue factor (TF), phosphatidylserine (PS), and thrombomodulin (TM)...
2017: PloS One
Xiaoyang Yi, Minxia Liu, Qun Luo, Hailong Zhuo, Hui Cao, Jiexi Wang, Ying Han
Dimethyl sulfoxide (DMSO) is widely used in biological studies as a cryoprotective agent for cells and tissues, and also for cryopreserved platelets (PLTs). However, few data on the toxic effects of DMSO following intravenous infusion of cryopreserved PLTs are available. The aim of this study was to explore dose-related effects of DMSO on red blood cells (RBCs), PLTs and vascular endothelial cells in vitro. The results showed that DMSO treatments had significant effects on RBCs, affecting osmotic fragility and increasing hemolysis...
April 2017: FEBS Open Bio
Ayaka Ohashi, Aya Murata, Yuichiro Cho, Shizuko Ichinose, Yuriko Sakamaki, Miwako Nishio, Osamu Hoshi, Silvia Fischer, Klaus T Preissner, Takatoshi Koyama
RNA may be released from vascular cells including endothelial cells in the event of injury and in vascular disease. Extracellular RNAs have been recognized as novel procoagulant and permeability-increasing factors. Extracellular RNA may function as inflammatory host alarm signals that serve to amplify the defense mechanism, but it may provide important links to thrombus formation. Extracellular RNA is degraded by RNase. We propose that RNase and its inhibitor RNase inhibitor (RI) act as modulators of homeostasis in the vasculature to control the functions of extracellular RNA...
2017: PloS One
Shu-Juan Li, Xiao-Juan Wang, Jing-Bo Hu, Xu-Qi Kang, Li Chen, Xiao-Ling Xu, Xiao-Ying Ying, Sai-Ping Jiang, Yong-Zhong Du
Acute lung injury (ALI) is a critical illness without effective therapeutic modalities currently. Recent studies indicated potential efficacy of statins for ALI, while high-dose statins was suggested to be significant for attenuating inflammation in vivo. Therefore, a lung-targeted drug delivery system (DDS) delivering simvastatin (SV) for ALI therapy was developed, attempting to improve the disease with a decreased dose and minimize potential adverse effects. SV-loaded nanostructured lipid carriers (SV/NLCs) with different size were prepared primarily...
November 2017: Drug Delivery
Yadan Zhang, Lijuan Song, Ruiyan Pan, Jianwei Gao, Bao-Xia Zang, Ming Jin
Hydroxysafflor yellow A (HSYA) is an effective ingredient of the Chinese herb Carthamus tinctorius L. The present study investigated the protective effect of HSYA on lipopolysaccharide (LPS)-induced acute respiratory distress syndrome in mice, and the underlying mechanisms involved. HSYA (14, 28, 56 mg/kg) was intraperitoneally injected to mice once daily from day 1 to 10 after LPS administration. HSYA attenuated the body weight loss, the augmented left index and the increase of pathologic changes in pulmonary inflammation caused by LPS...
2017: Biological & Pharmaceutical Bulletin
Guangxian Zhang, Xiancheng Zeng, Ren Zhang, Juan Liu, Weici Zhang, Yujun Zhao, Xiaoyuan Zhang, Zhixue Wu, Yuhui Tan, Yingya Wu, Biaoyan Du
BACKGROUND: Hepatocellular carcinoma (HCC) is the most commonly diagnosed malignancy of the liver, occurs frequently in the setting of chronic liver injury. Although multiple therapeutic approaches are available, the prognosis of patients with HCC remains poor. Dioscin is a natural steroid saponin that presents in various plants. The anti-cancer and anti-fibrotic effects have been extensively reported. However, the effect of dioscin on HCC remains unclear. We aimed to investigate the anti-HCC properties of dioscin in vitro and in vivo...
November 15, 2016: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Paula Abello-Cáceres, Javier Pizarro-Bauerle, Carlos Rosas, Ismael Maldonado, Lorena Aguilar-Guzmán, Carlos González, Galia Ramírez, Jorge Ferreira, Arturo Ferreira
BACKGROUND: For several decades now an antagonism between Trypanosoma cruzi infection and tumor development has been detected. The molecular basis of this phenomenon remained basically unknown until our proposal that T. cruzi Calreticulin (TcCRT), an endoplasmic reticulum-resident chaperone, translocated-externalized by the parasite, may mediate at least an important part of this effect. Thus, recombinant TcCRT (rTcCRT) has important in vivo antiangiogenic and antitumor activities. However, the relevant question whether the in vivo antitumor effect of T...
September 13, 2016: BMC Cancer
Misae Tsunaka, Reina Arai, Ayaka Ohashi, Takatoshi Koyama
OBJECTIVES: Combining vorinostat, L-asparaginase, and doxorubicin (Dox) led to improved response rates in the treatment of lymphoid tumors. However, deep-vein thrombosis has been noted as one of the most serious side effects with these drugs, and how these regimens cause deep-vein thrombosis is unclear. METHODS: We investigated the procoagulant effects of vorinostat, L-asparaginase, and doxorubicin in lymphoid tumors, focusing on tissue factor, phosphatidylserine, and antithrombin...
2016: SAGE Open Medicine
Yuelei Zhang, Junhui Yin, Hao Ding, Changqing Zhang, You-Shui Gao
Glucocorticoid has been reported to decrease blood vessel number and harm the blood supply in the femoral head, which is recognized to be an important mechanism of glucocorticoid-induced osteonecrosis of the femoral head (ONFH). To prevent glucocorticoid-induced ONFH, medication that promotes both bone formation and angiogenesis would be ideal. Vitamin K2 has been revealed to play an important role in bone metabolism; however, few studies have focused on the effect of Vitamin K2 on new vascular formation. Thus, this study aimed to investigate whether Vitamin K2 promoted new blood vessel formation in the presence of glucocorticoids, both in vitro and in vivo...
2016: International Journal of Biological Sciences
Xiaoqing Jiang, Bin Lv, Pan Li, Xianghui Ma, Ting Wang, Qian Zhou, Xiaoying Wang, Xiumei Gao
ETHNOPHARMACOLOGICAL RELEVANCE: Danhong injection (DHI) is a traditional Chinese medicine injection that has been widely used in therapy for cardiovascular diseases. However, neither its mechanism nor its active constituents are clearly understood. AIM OF THE STUDY: Our research aimed at identifying the anti-inflammatory ingredients and mechanism of DHI by combining high-throughput screening (HTS) with network pharmacology analysis. MATERIALS AND METHODS: The human endothelial cell line EAhy926 was cultured in vitro...
November 4, 2015: Journal of Ethnopharmacology
Ming Jin, Chun-Yan Sun, Bao-Xia Zang
OBJECTIVE: This study observed attenuating effect of hydroxysafflor yellow A (HSYA), an effective ingredient of aqueous extract of Carthamus tinctorius L, on lipopolysaccharide (LPS)-induced endothelium inflammatory injury. METHODS: Eahy926 human endothelium cell (EC) line was used; thiazolyl blue tetrazolium bromide (MTT) test was assayed to observe the viability of EC; Luciferase reporter gene assay was applied to measure nuclear factor-κB (NF-κB) p65 subunit nuclear binding activity in EC; Western blot technology was used to monitor mitogen activated protein kinase (MAPKs) and NF-κB activation...
January 2016: Chinese Journal of Integrative Medicine
Jing Suo, Long Zhao, Jian Wang, Zhichuan Zhu, Huafeng Zhang, Rong Gao
Oxidized low-density lipoprotein (ox-LDL) is well recognized to play a key role in the development of atherosclerosis. And influenza virus infection has been also recognized to promote the atherosclerosis onset and progressing. However, little is known about the mechanism into it. In present study, we investigated the infection of A/Porto Rico/8/1934 (H1N1) (PR8) influenza virus in human endothelial Eahy926 cells, and determined the induction of apoptosis by the virus infection in the cell. Then we investigated the apoptosis induced by ox-LDL in Eahy926 cells, determined the influence of influenza virus infection on the ox-LDL-induced apoptosis in Eahy926 cells...
July 2015: Journal of Medical Virology
Marzia Mura, Daniela Palmieri, Davide Garella, Antonella Di Stilo, Patrizia Perego, Giancarlo Cravotto, Domenico Palombo
This study proposes an alternative technique to prevent heat degradation induced by classic procedures of bioactive compound extraction, comparing classical maceration/decoction in hot water of polyphenols from Mango (Mangifera indica L.) (MI) with ultrasound-assisted extraction (UAE) in a water solution of β-cyclodextrin (β-CD) at room temperature and testing their biological activity on TNFα-induced endothelial dysfunction. Both extracts counteracted TNFα effects on EAhy926 cells, down-modulating interleukin-6, interleukin-8, cyclooxygenase-2 and intracellular adhesion molecule-1, while increasing endothelial nitric oxide synthase levels...
2015: Natural Product Research
A A Azarenok, A R Prochukhanova, V V Zenin, O G Liublinskaia, N M Kozlova, I N Zhilinskaia
The ability of the modern epidemic strains of influenza virus type A (subtypes H5N1, H3N2, H1N1pdm) and their surface proteins, hemagglutinin (HA) and neuraminidase to cause the activation of the cellular protein caspase-3 and stimulate the emergence of phosphatidylserine on the membrane of human endothelial cell line EAhy926 has been studied. It was questioned how the viruses and their surface proteins that were studied cause the activation of caspase-3 after 0.5 h of exposure that recorded immunogistotsitohimicheski...
2013: Tsitologiia
Na Wu, Na Gao, Dongying Fan, Jianchun Wei, Jing Zhang, Jing An
The pathogenesis of dengue virus (DENV) infection is not completely understood. Endothelial cells may act as a target of the virus and be involved in disease pathogenesis. Therefore, the identification of host cell components involved in DENV replication would provide useful information for better understanding DENV infection. In this study, a significantly decreased level of miR-223 was found in DENV2-infected EAhy926 cells, a human endothelial-like cell line, whereas miR-223 overexpression inhibited DENV2 replication...
November 2014: Microbes and Infection
I I Galkin, O Yu Pletjushkina, R A Zinovkin, V V Zakharova, I S Birjukov, B V Chernyak, E N Popova
Increased serum level of tumor necrosis factor α (TNFα) causes endothelial dysfunction and leads to serious vascular pathologies. TNFα signaling is known to involve reactive oxygen species (ROS). Using mitochondria-targeted antioxidant SkQR1, we studied the role of mitochondrial ROS in TNFα-induced apoptosis of human endothelial cell line EAhy926. We found that 0.2 nM SkQR1 prevents TNFα-induced apoptosis. SkQR1 has no influence on TNFα-dependent proteolytic activation of caspase-8 and Bid, but it inhibits cytochrome c release from mitochondria and cleavage of caspase-3 and its substrate PARP...
February 2014: Biochemistry. Biokhimii︠a︡
Tomoaki Kurosaki, Shigeru Kawakami, Yuriko Higuchi, Ryo Suzuki, Kazuo Maruyama, Hitoshi Sasaki, Fumiyoshi Yamashita, Mitsuru Hashida
Anionic bubble lipopolyplexes have been developed as anionic ultrasound (US)-responsive gene delivery carriers with biocompatible compounds for efficient and safe transfection in mice. The particles of the anionic bubble lipopolyplexes were approximately 450-600nm with an anionic surface charge. In the absence of US exposure, the bubble lipopolyplexes showed extremely low gene expression in the human vascular endothelial cell line EAhy926. The anionic bubble lipopolyplexes, however, delivered pDNA into cells without endocytosis and showed markedly high gene expression following US exposure...
February 28, 2014: Journal of Controlled Release: Official Journal of the Controlled Release Society
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