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Eahy926 cell line

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https://www.readbyqxmd.com/read/27765352/dioscin-suppresses-hepatocellular-carcinoma-tumor-growth-by-inducing-apoptosis-and-regulation-of-tp53-bax-bcl2-and-cleaved-casp3
#1
Guangxian Zhang, Xiancheng Zeng, Ren Zhang, Juan Liu, Weici Zhang, Yujun Zhao, Xiaoyuan Zhang, Zhixue Wu, Yuhui Tan, Yingya Wu, Biaoyan Du
BACKGROUND: Hepatocellular carcinoma (HCC) is the most commonly diagnosed malignancy of the liver, occurs frequently in the setting of chronic liver injury. Although multiple therapeutic approaches are available, the prognosis of patients with HCC remains poor. Dioscin is a natural steroid saponin that presents in various plants. The anti-cancer and anti-fibrotic effects have been extensively reported. However, the effect of dioscin on HCC remains unclear. We aimed to investigate the anti-HCC properties of dioscin in vitro and in vivo...
November 15, 2016: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/27619675/does-native-trypanosoma-cruzi-calreticulin-mediate-growth-inhibition-of-a-mammary-tumor-during-infection
#2
Paula Abello-Cáceres, Javier Pizarro-Bauerle, Carlos Rosas, Ismael Maldonado, Lorena Aguilar-Guzmán, Carlos González, Galia Ramírez, Jorge Ferreira, Arturo Ferreira
BACKGROUND: For several decades now an antagonism between Trypanosoma cruzi infection and tumor development has been detected. The molecular basis of this phenomenon remained basically unknown until our proposal that T. cruzi Calreticulin (TcCRT), an endoplasmic reticulum-resident chaperone, translocated-externalized by the parasite, may mediate at least an important part of this effect. Thus, recombinant TcCRT (rTcCRT) has important in vivo antiangiogenic and antitumor activities. However, the relevant question whether the in vivo antitumor effect of T...
2016: BMC Cancer
https://www.readbyqxmd.com/read/27504186/cell-based-laboratory-evaluation-of-coagulation-activation-by-antineoplastic-drugs-for-the-treatment-of-lymphoid-tumors
#3
Misae Tsunaka, Reina Arai, Ayaka Ohashi, Takatoshi Koyama
OBJECTIVES: Combining vorinostat, L-asparaginase, and doxorubicin (Dox) led to improved response rates in the treatment of lymphoid tumors. However, deep-vein thrombosis has been noted as one of the most serious side effects with these drugs, and how these regimens cause deep-vein thrombosis is unclear. METHODS: We investigated the procoagulant effects of vorinostat, L-asparaginase, and doxorubicin in lymphoid tumors, focusing on tissue factor, phosphatidylserine, and antithrombin...
2016: SAGE Open Medicine
https://www.readbyqxmd.com/read/27313492/vitamin-k2-ameliorates-damage-of-blood-vessels-by-glucocorticoid-a-potential-mechanism-for-its-protective-effects-in-glucocorticoid-induced-osteonecrosis-of-the-femoral-head-in-a-rat-model
#4
Yuelei Zhang, Junhui Yin, Hao Ding, Changqing Zhang, You-Shui Gao
Glucocorticoid has been reported to decrease blood vessel number and harm the blood supply in the femoral head, which is recognized to be an important mechanism of glucocorticoid-induced osteonecrosis of the femoral head (ONFH). To prevent glucocorticoid-induced ONFH, medication that promotes both bone formation and angiogenesis would be ideal. Vitamin K2 has been revealed to play an important role in bone metabolism; however, few studies have focused on the effect of Vitamin K2 on new vascular formation. Thus, this study aimed to investigate whether Vitamin K2 promoted new blood vessel formation in the presence of glucocorticoids, both in vitro and in vivo...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/26319960/bioactivity-integrated-uplc-q-tof-ms-of-danhong-injection-to-identify-nf-%C3%AE%C2%BAb-inhibitors-and-anti-inflammatory-targets-based-on-endothelial-cell-culture-and-network-pharmacology
#5
Xiaoqing Jiang, Bin Lv, Pan Li, Xianghui Ma, Ting Wang, Qian Zhou, Xiaoying Wang, Xiumei Gao
ETHNOPHARMACOLOGICAL RELEVANCE: Danhong injection (DHI) is a traditional Chinese medicine injection that has been widely used in therapy for cardiovascular diseases. However, neither its mechanism nor its active constituents are clearly understood. AIM OF THE STUDY: Our research aimed at identifying the anti-inflammatory ingredients and mechanism of DHI by combining high-throughput screening (HTS) with network pharmacology analysis. MATERIALS AND METHODS: The human endothelial cell line EAhy926 was cultured in vitro...
November 4, 2015: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/26015073/hydroxysafflor-yellow-a-attenuate-lipopolysaccharide-induced-endothelium-inflammatory-injury
#6
Ming Jin, Chun-Yan Sun, Bao-Xia Zang
OBJECTIVE: This study observed attenuating effect of hydroxysafflor yellow A (HSYA), an effective ingredient of aqueous extract of Carthamus tinctorius L, on lipopolysaccharide (LPS)-induced endothelium inflammatory injury. METHODS: Eahy926 human endothelium cell (EC) line was used; thiazolyl blue tetrazolium bromide (MTT) test was assayed to observe the viability of EC; Luciferase reporter gene assay was applied to measure nuclear factor-κB (NF-κB) p65 subunit nuclear binding activity in EC; Western blot technology was used to monitor mitogen activated protein kinase (MAPKs) and NF-κB activation...
January 2016: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/25777161/influenza-virus-aggravates-the-ox-ldl-induced-apoptosis-of-human-endothelial-cells-via-promoting-p53-signaling
#7
Jing Suo, Long Zhao, Jian Wang, Zhichuan Zhu, Huafeng Zhang, Rong Gao
Oxidized low-density lipoprotein (ox-LDL) is well recognized to play a key role in the development of atherosclerosis. And influenza virus infection has been also recognized to promote the atherosclerosis onset and progressing. However, little is known about the mechanism into it. In present study, we investigated the infection of A/Porto Rico/8/1934 (H1N1) (PR8) influenza virus in human endothelial Eahy926 cells, and determined the induction of apoptosis by the virus infection in the cell. Then we investigated the apoptosis induced by ox-LDL in Eahy926 cells, determined the influence of influenza virus infection on the ox-LDL-induced apoptosis in Eahy926 cells...
July 2015: Journal of Medical Virology
https://www.readbyqxmd.com/read/25589236/simultaneous-ultrasound-assisted-water-extraction-and-%C3%AE-cyclodextrin-encapsulation-of-polyphenols-from-mangifera-indica-stem-bark-in-counteracting-tnf%C3%AE-induced-endothelial-dysfunction
#8
Marzia Mura, Daniela Palmieri, Davide Garella, Antonella Di Stilo, Patrizia Perego, Giancarlo Cravotto, Domenico Palombo
This study proposes an alternative technique to prevent heat degradation induced by classic procedures of bioactive compound extraction, comparing classical maceration/decoction in hot water of polyphenols from Mango (Mangifera indica L.) (MI) with ultrasound-assisted extraction (UAE) in a water solution of β-cyclodextrin (β-CD) at room temperature and testing their biological activity on TNFα-induced endothelial dysfunction. Both extracts counteracted TNFα effects on EAhy926 cells, down-modulating interleukin-6, interleukin-8, cyclooxygenase-2 and intracellular adhesion molecule-1, while increasing endothelial nitric oxide synthase levels...
2015: Natural Product Research
https://www.readbyqxmd.com/read/25509110/-ability-of-the-influenza-viruses-and-their-envelope-proteins-to-stimulates-endothelial-cells-apoptosis-in-vitro
#9
A A Azarenok, A R Prochukhanova, V V Zenin, O G Liublinskaia, N M Kozlova, I N Zhilinskaia
The ability of the modern epidemic strains of influenza virus type A (subtypes H5N1, H3N2, H1N1pdm) and their surface proteins, hemagglutinin (HA) and neuraminidase to cause the activation of the cellular protein caspase-3 and stimulate the emergence of phosphatidylserine on the membrane of human endothelial cell line EAhy926 has been studied. It was questioned how the viruses and their surface proteins that were studied cause the activation of caspase-3 after 0.5 h of exposure that recorded immunogistotsitohimicheski...
2013: Tsitologiia
https://www.readbyqxmd.com/read/25181337/mir-223-inhibits-dengue-virus-replication-by-negatively-regulating-the-microtubule-destabilizing-protein-stmn1-in-eahy926%C3%A2-cells
#10
Na Wu, Na Gao, Dongying Fan, Jianchun Wei, Jing Zhang, Jing An
The pathogenesis of dengue virus (DENV) infection is not completely understood. Endothelial cells may act as a target of the virus and be involved in disease pathogenesis. Therefore, the identification of host cell components involved in DENV replication would provide useful information for better understanding DENV infection. In this study, a significantly decreased level of miR-223 was found in DENV2-infected EAhy926 cells, a human endothelial-like cell line, whereas miR-223 overexpression inhibited DENV2 replication...
November 2014: Microbes and Infection
https://www.readbyqxmd.com/read/24794727/mitochondria-targeted-antioxidants-prevent-tnf%C3%AE-induced-endothelial-cell-damage
#11
I I Galkin, O Yu Pletjushkina, R A Zinovkin, V V Zakharova, I S Birjukov, B V Chernyak, E N Popova
Increased serum level of tumor necrosis factor α (TNFα) causes endothelial dysfunction and leads to serious vascular pathologies. TNFα signaling is known to involve reactive oxygen species (ROS). Using mitochondria-targeted antioxidant SkQR1, we studied the role of mitochondrial ROS in TNFα-induced apoptosis of human endothelial cell line EAhy926. We found that 0.2 nM SkQR1 prevents TNFα-induced apoptosis. SkQR1 has no influence on TNFα-dependent proteolytic activation of caspase-8 and Bid, but it inhibits cytochrome c release from mitochondria and cleavage of caspase-3 and its substrate PARP...
February 2014: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/24384299/development-of-anionic-bubble-lipopolyplexes-for-efficient-and-safe-gene-transfection-with-ultrasound-exposure-in-mice
#12
Tomoaki Kurosaki, Shigeru Kawakami, Yuriko Higuchi, Ryo Suzuki, Kazuo Maruyama, Hitoshi Sasaki, Fumiyoshi Yamashita, Mitsuru Hashida
Anionic bubble lipopolyplexes have been developed as anionic ultrasound (US)-responsive gene delivery carriers with biocompatible compounds for efficient and safe transfection in mice. The particles of the anionic bubble lipopolyplexes were approximately 450-600nm with an anionic surface charge. In the absence of US exposure, the bubble lipopolyplexes showed extremely low gene expression in the human vascular endothelial cell line EAhy926. The anionic bubble lipopolyplexes, however, delivered pDNA into cells without endocytosis and showed markedly high gene expression following US exposure...
February 28, 2014: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/24377092/suitability-of-cell-based-label-free-detection-for-cytotoxicity-screening-of-carbon-nanotubes
#13
Claudia Meindl, Markus Absenger, Eva Roblegg, Eleonore Fröhlich
Cytotoxicity testing of nanoparticles (NPs) by conventional screening assays is often complicated by interference. Carbon nanotubes (CNTs) are particularly difficult to assess. To test the suitability of cell-based label-free techniques for this application, a panel of CNTs with different diameters and surface functionalizations was assessed by impedance-based technique (xCELLigence RTCA) and automated microscopy (Cell-IQ) compared to formazan bioreduction (MTS assay). For validation of the label-free systems different concentrations of ethanol and of amine (AMI) polystyrene NPs were used...
2013: BioMed Research International
https://www.readbyqxmd.com/read/24376792/high-glucose-induced-increased-expression-of-endothelin-1-in-human-endothelial-cells-is-mediated-by-activated-ccaat-enhancer-binding-proteins
#14
Simona-Adriana Manea, Andra Todirita, Adrian Manea
High glucose-induced endothelial dysfunction is partially mediated by the down-stream pathophysiological effects triggered by increased expression of endothelin-1 (ET-1). The molecular control mechanisms of ET-1 synthesis are yet to be discovered. Members of the CCAAT/enhancer-binding proteins (C/EBP) family are important regulators of key metabolic processes, cellular differentiation and proinflammatory genes. In this study, we aimed at elucidating the role of C/EBP in mediating the high glucose effect on ET-1 expression in human endothelial cells (EC)...
2013: PloS One
https://www.readbyqxmd.com/read/24374813/serum-deprivation-elevates-the-levels-of-microvesicles-with-different-size-distributions-and-selectively-enriched-proteins-in-human-myeloma-cells-in-vitro
#15
Li Sun, Hong-xiang Wang, Xiao-jian Zhu, Pin-hui Wu, Wei-qun Chen, Ping Zou, Qiu-bai Li, Zhi-chao Chen
AIM: To investigate the effects of serum deprivation (SD) on microvesicles (MVs) secreted from human myeloma cells and the implications for disease progression. METHODS: RPMI 8226, U266, and KM3 human myeloma cells were incubated in medium containing 10% (non-SD) or 1% fetal bovine serum (SD) and MVs were isolated. The levels and size distribution of MVs were analyzed with flow cytometry. The protein profiles of MVs were studied using 2D SDS-PAGE, MALDI-TOF-MS, and Western blotting...
March 2014: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/24252910/hsa-let-7g-mirna-targets-caspase-3-and-inhibits-the-apoptosis-induced-by-ox-ldl-in-endothelial-cells
#16
Yefei Zhang, Naiyun Chen, Jihao Zhang, Yaling Tong
It has been well confirmed ox-LDL plays key roles in the development of atherosclerosis via binding to LOX-1 and inducing apoptosis in vascular endothelial cells. Recent studies have shown ox-LDL can suppress microRNA has-let-7g, which in turn inhibits the ox-LDL induced apoptosis. However, details need to be uncovered. To determine the anti-atherosclerosis effect of microRNA has-let-7g, and to evaluate the possibility of CASP3 as an anti-atherosclerotic drug target by has-let-7g, the present study determined the role of hsa-let-7g miRNA in ox-LDL induced apoptosis in the vascular endothelial cells...
2013: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/24002468/toward-computer-simulation-of-high-let-in-vitro-survival-curves
#17
A-C Heuskin, C Michiels, S Lucas
We developed a Monte Carlo based computer program called MCSC (Monte Carlo Survival Curve) able to predict the survival fraction of cells irradiated in vitro with a broad beam of high linear energy transfer particles. Three types of cell responses are studied: the usual high dose response, the bystander effect and the low-dose hypersensitivity (HRS). The program models the broad beam irradiation and double strand break distribution following Poisson statistics. The progression of cells through the cell cycle is taken into account while the repair takes place...
September 21, 2013: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/23696885/activation-of-coagulation-by-lenalidomide-based-regimens-for-the-treatment-of-multiple-myeloma
#18
Yu Isozumi, Reina Arai, Kazumi Fujimoto, Takatoshi Koyama
We investigated the procoagulant effects of lenalidomide (Len)-based regimens in vitro focusing on tissue factor (TF) and phosphatidylserine (PS). We examined the effects of a pharmacological concentration of Len with or without the corticosteroid dexamethasone (Dex) and the proteasome inhibitor bortezomib (Bor) using the human vascular endothelial cell line EAhy926 and the monocytic cell lines THP-1 and U937. Cell-surface procoagulant activity (PCA) was induced by Dex-containing regimens in all lines. Expression of TF antigen on the cell surface and of TF mRNA was markedly increased by Dex-containing regimens...
2013: PloS One
https://www.readbyqxmd.com/read/23337791/effect-of-mild-hypothermia-on-breast-cancer-cells-adhesion-and-migration
#19
X M Zhang, Y G Lv, G B Chen, Y Zou, C W Lin, L Yang, P Guo, M P Lin
To explore the effect of mild hypothermia (35ºC) on breast cancer cells adhesion to vascular endothelial cells, a parallel plant flow chamber was used to observe the adhesion of human breast cancer cells MDA-MB-231 to endothelial cells Eahy926 under physiological flow at 35ºC and 37ºC, as well as the role of intercellular adhesion molecule 1 (ICAM-1) in this process. Further, the effect of mild hypothermia (35ºC) on migration of MDA-MB-231 was also studied. Our results show that mild hypothermia can inhibit the adhesion of tumor cells to endothelial cells and ICAM-1 plays an important role in this process...
December 2012: Bioscience Trends
https://www.readbyqxmd.com/read/22789069/action-of-polystyrene-nanoparticles-of-different-sizes-on-lysosomal-function-and-integrity
#20
COMPARATIVE STUDY
Eleonore Fröhlich, Claudia Meindl, Eva Roblegg, Birgit Ebner, Markus Absenger, Thomas R Pieber
BACKGROUND: Data from environmental exposure to nanoparticles (NPs) suggest that chronic exposure may increase the incidence of lung, cardiovascular and neurodegenerative diseases. Impairment of cell function by intracellular accumulation of NPs is also suspected. Many types of NPs have been detected in the endosomal-lysosomal system and, upon repeated exposure, alterations of the endosomal-lysosomal system may occur. To identify such effects we compared the effect of carboxyl polystyrene particles (CPS) of different sizes (20-500 nm) on lysosomes of the endothelial cell line EAhy926 after short (24h) and long (72h-96h) exposure times...
July 12, 2012: Particle and Fibre Toxicology
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