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https://www.readbyqxmd.com/read/28315306/molecular-characterization-of-camelpox-virus-isolates-from-bikaner-india-evidence-of-its-endemicity
#1
Shyam Singh Dahiya, Sachin Kumar, Sharat Chandra Mehta, Raghvendar Singh, Kashi Nath, Shirish D Narnaware, Fateh Chand Tuteja
Camelpox is an important viral disease of camels, which may produce mild skin lesions or severe systemic infections. Camelpox virus (CMLV) isolates retrieved from an incidence of camelpox in camels at Bikaner, India were characterized on the basis of genotype and pathotype. Histopathological examination of the CMLV scab revealed intracytoplasmic-eosinophilic inclusion bodies. The phylogenetic analysis of all eight CMLV isolates for C18L gene nucleotide sequence revealed its clustering with its strains M-96 from Kazakhstan and CMS from Iran...
March 14, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28301489/comparative-analysis-of-fecal-microbial-communities-in-cattle-and-bactrian-camels
#2
Liang Ming, Li Yi, Siriguleng, Surong Hasi, Jing He, Le Hai, Zhaoxia Wang, Fucheng Guo, Xiangyu Qiao, Jirimutu
Bactrian camels may have a unique gastrointestinal (GI) microbiome because of their distinctive digestive systems, unique eating habits and extreme living conditions. However, understanding of the microbial communities in the Bactrian camel GI tract is still limited. In this study, microbial communities were investigated by comparative analyses of 16S rRNA hypervariable region V4 sequences of fecal bacteria sampled from 94 animals in four population groups: Inner Mongolian cattle (IMG-Cattle), Inner Mongolian domestic Bactrian camels (IMG-DBC), Mongolian domestic Bactrian camels (MG-DBC), and Mongolian wild Bactrian camels (MG-WBC)...
2017: PloS One
https://www.readbyqxmd.com/read/28293445/microscopic-and-molecular-detection-of-camel-piroplasmosis-in-gadarif-state-sudan
#3
Abdalla Mohamed Ibrahim, Ahmed A H Kadle, Hamisi Said Nyingilili
The socioeconomic importance of camels (Camelus dromedarius) could not be neglected in the Sudan. The present study was planned to confirm the presence of piroplasmosis in camels from the Eastern region of the Sudan (Gedarif State) using microscopical (blood film) and molecular technique (PCR). A total of 55 camels of different sexes (34 females and 21 males) were sampled from four localities of the state between January 2011 and January 2012. The prevalence rates using parasitological and molecular examinations were 43...
2017: Veterinary Medicine International
https://www.readbyqxmd.com/read/28282952/a%C3%A2-frameshift%C3%A2-mutation%C3%A2-in%C3%A2-kit%C3%A2-is%C3%A2-associated%C3%A2-with%C3%A2-white%C3%A2-spotting%C3%A2-in%C3%A2-the%C3%A2-arabian%C3%A2-camel
#4
Heather Holl, Ramiro Isaza, Yasmin Mohamoud, Ayeda Ahmed, Faisal Almathen, Cherifi Youcef, Semir Gaouar, Douglas F Antczak, Samantha Brooks
While the typical Arabian camel is characterized by a single colored coat, there are rare populations with white spotting patterns. White spotting coat patterns are found in virtually all domesticated species, but are rare in wild species. Theories suggest that white spotting is linked to the domestication process, and is occasionally associated with health disorders. Though mutations have been found in a diverse array of species, fewer than 30 genes have been associated with spotting patterns, thus providing a key set of candidate genes for the Arabian camel...
March 9, 2017: Genes
https://www.readbyqxmd.com/read/28271570/single-domain-antibodies-for-the-knockdown-of-cytosolic-and-nuclear-proteins
#5
REVIEW
Thomas Böldicke
Single domain antibodies (sdAbs) from camels or sharks comprise only the variable heavy chain domain. Human sdAbs comprise the variable domain of the heavy chain (VH) or light chain (VL) and can be selected from human antibodies. SdAbs are stable, non aggregating molecules in vitro and in vivo compared to complete antibodies and scFv fragments. They are excellent novel inhibitors of cytosolic/nuclear proteins because they are correctly folded inside the cytosol in contrast to scFv fragments. SdAbs are unique because of their excellent specificity and possibility to target posttranslational modifications such as phosphorylation sites, conformers or interaction regions of proteins that cannot be targeted with genetic knockout techniques and are impossible to knockdown with RNAi...
March 8, 2017: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/28266172/n-glycosylation-proteomic-characterization-and-cross-species-comparison-of-milk-whey-proteins-from-dairy-animals
#6
Yongxin Yang, Nan Zheng, Xiaowei Zhao, Yangdong Zhang, Rongwei Han, Shengguo Zhao, Jinhui Yang, Songli Li, Tongjun Guo, Changjiang Zang, Jiaqi Wang
Glycosylated proteins in milk have been implicated in multiple biological roles. However, the N-glycoprotein components and their complexity in milk whey from dairy animals are not well characterized. Here, a modified proteomics approach consisting of N-glycopeptide enrichment and identification was used to map the N-glycoproteome profile of milk whey from Holstein and Jersey cows, buffaloes, yaks, goats, camels, and horses. A total of 233 N-glycosylation sites, corresponding to 147 N-glycoproteins, were detected in the studied animals...
March 7, 2017: Proteomics
https://www.readbyqxmd.com/read/28246453/preparation-of-goat-and-rabbit-anti-camel-immunoglobulin-g-whole-molecule-labeled-with-horseradish-peroxidase
#7
Eman Hussein Abdel-Rahman, Jakeen Kamal El-Jakee, Mahmoud Essam Hatem, Nagwa Sayed Ata, Ehab Ali Fouad
AIM: As the labeled anti-camel immunoglobulins (Igs) with enzymes for enzyme-linked immunosorbent assay (ELISA) are unavailable in the Egyptian market, the present investigation was directed for developing local labeled anti-camel IgG with horseradish peroxidase (HRP) to save hard curacy. MATERIALS AND METHODS: For purification of camel IgG whole molecule, camel sera was preliminary precipitated with 50% saturated ammonium sulfate and dialyzed against 15 mM phosphate-buffered saline pH 7...
January 2017: Veterinary World
https://www.readbyqxmd.com/read/28242943/rheological-and-physical-properties-of-camel-and-cow-milk-gels-enriched-with-phosphate-and-calcium-during-acid-induced-gelation
#8
Mohammad Kamal, Mohammed Foukani, Romdhane Karoui
The rheological properties of acid-induced coagulation of camel and cow milk gels following the addition of calcium chloride (CaCl2) and hydrogen phosphate dehydrate (Na2HPO4*2H2O) were investigated using a dynamic low amplitude oscillatory rheology. For a considered condition, the final values of storage modulus (G') and loss modulus (G″) of camel milk gels were significantly lower than those of cow milk gels. The increase of the added CaCl2 levels improved significantly the gelation properties of camel and cow milk gels, since a reduction in the gelation time and an increase in the gel firmness were observed...
February 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28242041/a-novel-snapback-primer-probe-assay-for-the-detection-and-discrimination-of-sympatric-haemonchus-species-using-dna-melting-analysis
#9
Rudolf Pichler, Katja Silbermayr, Kathiravan Periasamy
Different sympatric species of Haemonchus parasites infecting ruminants and camels can be distinguished morphologically, but involves tedious microscopic examinations, measurements and several other limitations. Information on internal transcribed spacer-2 (ITS-2) sequence provides confirmatory differentiation of sympatric Haemonchus species. The present study introduces a novel, snapback primer probe based, real time PCR assay for the differentiation of three sympatric Haemonchus species, H. contortus (Hco), H...
February 20, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28240229/proteomic-analysis-and-cross-species-comparison-of-casein-fractions-from-the-milk-of-dairy-animals
#10
Xiaxia Wang, Xiaowei Zhao, Dongwei Huang, Xiaocheng Pan, Yunxia Qi, Yongxin Yang, Huiling Zhao, Guanglong Cheng
Casein micelles contribute to the physicochemical properties of milk and may also influence its functionality. At present, however, there is an incomplete understanding of the casein micelle associated proteins and its diversity among the milk obtained from different species. Therefore, milk samples were collected from seven dairy animals groups, casein fractions were prepared by ultracentrifugation and their constituent proteins were identified by liquid chromatography tandem mass spectrometry. A total of 193 distinct proteins were identified among all the casein micelle preparations...
February 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28239627/self-administration-of-smokeless-tobacco-products-in-rats
#11
Mark G LeSage, Danielle Burroughs, Peter Muelken, Andrew C Harris
OBJECTIVE: Preclinical abuse liability assessment is an essential component of tobacco regulatory science. The goal of this project was to evaluate the relative abuse liability of smokeless tobacco products in rats using aqueous extracts of those products. These extracts provide exposure to an extensive range of nicotine and non-nicotine tobacco constituents as occurs in humans. METHODS: Rats were trained to self-administer either nicotine alone or extracts of Camel Snus or Kodiak smokeless tobacco at an equivalent nicotine unit dose...
October 2016: Tobacco Regulatory Science
https://www.readbyqxmd.com/read/28221127/serologic-evidence-for-mers-cov-infection-in-dromedary-camels-punjab-pakistan-2012-2015
#12
Muhammad Saqib, Andrea Sieberg, Muhammad Hammad Hussain, Muhammad Khalid Mansoor, Ali Zohaib, Erik Lattwein, Marcel Alexander Müller, Christian Drosten, Victor Max Corman
Dromedary camels from Africa and Arabia are an established source for zoonotic Middle East respiratory syndrome coronavirus (MERS-CoV) infection among humans. In Pakistan, we found specific neutralizing antibodies in samples from 39.5% of 565 dromedaries, documenting significant expansion of the enzootic range of MERS-CoV to Asia.
March 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28219371/erratum-to-camel-milk-peptide-improves-wound-healing-in-diabetic-rats-by-orchestrating-the-redox-status-and-immune-response
#13
Hossam Ebaid, Bahaa Abdel-Salam, Iftekhar Hassan, Jameel Al-Tamimi, Ali Metwalli, Ibrahim Alhazza
No abstract text is available yet for this article.
February 20, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28202080/mentholation-affects-the-cigarette-microbiota-by-selecting-for-bacteria-resistant-to-harsh-environmental-conditions-and-selecting-against-potential-bacterial-pathogens
#14
Jessica Chopyk, Suhana Chattopadhyay, Prachi Kulkarni, Emma Claye, Kelsey R Babik, Molly C Reid, Eoghan M Smyth, Lauren E Hittle, Joseph N Paulson, Raul Cruz-Cano, Mihai Pop, Stephanie S Buehler, Pamela I Clark, Amy R Sapkota, Emmanuel F Mongodin
BACKGROUND: There is a paucity of data regarding the microbial constituents of tobacco products and their impacts on public health. Moreover, there has been no comparative characterization performed on the bacterial microbiota associated with the addition of menthol, an additive that has been used by tobacco manufacturers for nearly a century. To address this knowledge gap, we conducted bacterial community profiling on tobacco from user- and custom-mentholated/non-mentholated cigarette pairs, as well as a commercially-mentholated product...
February 15, 2017: Microbiome
https://www.readbyqxmd.com/read/28188640/rennet-induced-coagulation-of-raw-and-heated-camel-and-cow-milk-gels-determined-by-instrumental-techniques-effects-of-added-calcium-and-phosphate
#15
Romdhane Karoui, Mohammad Kamal
BACKGROUND: The potentiality of the front-face fluorescence spectroscopy and rheological measurements combined with chemometric tools to characterize the structure evolution during coagulation of raw and preheated camel and cow milk at 50 and 70 °C with/without added calcium and phosphate was evaluated. Tryptophan and vitamin A fluorescence spectra were collected during the gelation of milk at 0, 10, 20, 30, 40, and 45 min after the addition of rennet-induced coagulation. In parallel, the storage modulus (G'), loss modulus (G") and tan delta (tan δ) were determined using low amplitude oscillation shear analysis...
February 11, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28185821/public-response-to-mers-cov-in-the-middle-east-iphone-survey-in-six-countries
#16
Amani S Alqahtani, Harunor Rashid, Mada H Basyouni, Tariq M Alhawassi, Nasser F BinDhim
Gulf Cooperation Council (GCC) countries bear the heaviest brunt of MERS-CoV. This study aims to compare public awareness and practice around MERS-CoV across GCC countries. A cross-sectional survey was conducted using the Gulf Indicators (GI) smartphone app among people in the six GCC countries, namely Saudi Arabia, Kuwait, the United Arab Emirates, Qatar, Bahrain, and Oman. A total of 1812 participants recruited. All were aware of MERS-CoV, yet the perception and practice around MERS-CoV varied widely between countries...
February 6, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28179025/the-role-of-nucularia-perrinii-batt-chenopodiaceae-in-the-camel-based-sahrawi-social-ecological-system
#17
Gabriele Volpato, Antonello Di Nardo
BACKGROUND: Pastoral social-ecological systems (SESs) are adaptive and complex systems rooted in the extensive exploitation of forage plants for livestock-based livelihoods and culture. There are species and relations that are foundational to the existence of these SESs. Nucularia perrinii Batt. (Chenopodiaceae) is an endemic halophyte plant of central and western Sahara seldom cited in the scientific literature. The objective of this study was to investigate the role of this plant in the SES of the Sahrawi camel nomads of Western Sahara...
February 8, 2017: Journal of Ethnobiology and Ethnomedicine
https://www.readbyqxmd.com/read/28177177/purification-and-quantification-of-heavy-chain-antibodies-from-the-milk-of-bactrian-camels
#18
Hongqiang Yao, Min Zhang, Yi Li, Jirimutu Yao, He Meng, Siriguleng Yu
Camel milk has a unique composition with naturally occurring heavy-chain antibodies (HCAbs), which exert rehabilitating potencies in infection and immunity. To characterize HCAb in camel milk, immunoglobulin G (IgG) was isolated from the milk of Camelus bactrianus by a combination of affinity chromatography and sodium dodecyl sulfate polyacrylamide gel electrophoresis to purify and size-fractionate protein A and protein G, which were further identified by Western blotting, and were quantified by bicinchoninic acid (BCA) and ELISA...
February 8, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28176495/serosurvey-of-coxiella-burnetii-q-fever-in-dromedary-camels-camelus-dromedarius-in-laikipia-county-kenya
#19
A S Browne, E M Fèvre, M Kinnaird, D M Muloi, C A Wang, P S Larsen, T O'Brien, S L Deem
Dromedary camels (Camelus dromedarius) are an important protein source for people in semi-arid and arid regions of Africa. In Kenya, camel populations have grown dramatically in the past few decades resulting in the potential for increased disease transmission between humans and camels. An estimated four million Kenyans drink unpasteurized camel milk, which poses a disease risk. We evaluated the seroprevalence of a significant zoonotic pathogen, Coxiella burnetii (Q fever), among 334 camels from nine herds in Laikipia County, Kenya...
February 8, 2017: Zoonoses and Public Health
https://www.readbyqxmd.com/read/28176384/the-effect-of-some-cryoprotectants-on-dromedary-camel-frozen-thawed-semen
#20
D A El-Badry, R H Mohamed, H A El-Metwally, T R Abo Al-Naga
The cryopreserved camel semen is often associated with poor quality and fertility. This study aimed to improve the dromedary frozen semen quality by comparing the efficiency of four cryoprotectant agents (CPAs) on sperm freezability. Semen samples were collected from seven male Maghrabi camels, diluted with Shotor diluent supplemented with glycerol (Sh-G), dimethyl formamide (DMF, Sh-DF), dimethyl sulfoxide (DMSO, Sh-DS) or ethylene glycol (EG, Sh-EG), all at 6% final concentration, and the samples were subjected to cryopreservation...
February 7, 2017: Reproduction in Domestic Animals, Zuchthygiene
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