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https://www.readbyqxmd.com/read/28634355/longitudinal-study-of-middle-east-respiratory-syndrome-coronavirus-infection-in-dromedary-camel-herds-in-saudi-arabia-2014-2015
#1
Maged Gomaa Hemida, Abdulmohsen Alnaeem, Daniel Kw Chu, Ranawaka Apm Perera, Samuel Ms Chan, Faisal Almathen, Emily Yau, Brian Cy Ng, Richard J Webby, Leo Lm Poon, Malik Peiris
Two herds of dromedary camels were longitudinally sampled with nasal and rectal swabs and serum, between September 2014 and May 2015, and the samples were tested for Middle East Respiratory Syndrome (MERS) coronavirus RNA and antibodies. Evidence of MERS-CoV infection was confirmed in one herd on the basis of detection of virus RNA in nasal swabs from three camels and significant increases in the antibody titers from three others. The three viruses were genetically identical, thus indicating introduction of a single virus into this herd...
June 21, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28628400/west-nile-virus-seroprevalence-in-animals-in-palestine-and-israel
#2
Kifaya Azmi, Sharon Tirosh-Levy, Mu'taz Manasrah, Rotem Mizrahi, Abed Nasereddin, Amer Al-Jawabreh, Suheir Ereqat, Ziad Abdeen, Yaniv Lustig, Boris Gelman, Gili Schvartz, Amir Steinman
West Nile virus (WNV) epidemiological situation in Israel and Palestine, due to their unique location, draws attention following to the global spread of West Nile fever (WNF). Although much information is available from Israel on clinical cases and prevalence of WNV, clinical cases are rarely reported in Palestine, and prevalence is not known. The objectives of this study were to determine WNV seroprevalence in various domestic animals in Palestine and to reevaluate current seroprevalence, force of infection, and risk factors for WNV exposure in horses in Israel...
June 19, 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/28620626/tobacco-smoke-constituents-trigger-cytoplasmic-calcium-release
#3
M Flori Sassano, Arunava Ghosh, Robert Tarran
Cytosolic Ca(2+) is a universal second messenger that is involved in many processes throughout the body, including the regulation of cell growth/cell division, apoptosis, and the secretion of both ions, and macromolecules. Tobacco smoke exerts multiple effects on airway epithelia and we have previously shown that Kentucky reference cigarette smoke exposure elevated the second messenger Ca(2+), leading to dysfunctional ion secretion. In this study, we tested whether little cigar and commercial cigarette smoke exposure exerts similar effects on intracellular Ca(2+) levels...
June 1, 2017: Applied in Vitro Toxicology
https://www.readbyqxmd.com/read/28617666/parasitic-diseases-of-camels-in-iran-1931-2017-a-literature-review
#4
Alireza Sazmand, Anja Joachim
Parasitic diseases of camels are major causes of impaired milk and meat production, decreases in performance or even death. Some camel parasites also represent a threat to human health. About 171,500 one-humped camels (Camelus dromedarius) and 100-300 two-humped camels (Camelus bactrianus) live in Iran. Knowledge of the biodiversity of their parasites is still limited. The present review covers all information about camel parasitic diseases in Iran published as dissertations and in both Iranian and international journals from 1931 to February 2017...
2017: Parasite: Journal de la Société Française de Parasitologie
https://www.readbyqxmd.com/read/28616502/dromedary-camels-in-northern-mali-have-high-seropositivity-to-mers-cov
#5
Darryl Falzarano, Badian Kamissoko, Emmie de Wit, Ousmane Maïga, Jacqueline Cronin, Kassim Samaké, Abdalah Traoré, Shauna Milne-Price, Vincent J Munster, Nafomon Sogoba, Mamadou Niang, David Safronetz, Heinz Feldmann
A high percentage (up to 90%) of dromedary camels in the Middle East as well as eastern and central Africa have antibodies to Middle East respiratory syndrome coronavirus (MERS-CoV). Here we report comparably high positivity of MERS-CoV antibodies in dromedary camels from northern Mali. This extends the range of MERS-CoV further west in Africa than reported to date and cautions that MERS-CoV should be considered in cases of severe respiratory disease in the region.
June 2017: One Health
https://www.readbyqxmd.com/read/28616497/mers-coronavirus-from-discovery-to-intervention
#6
REVIEW
W Widagdo, Nisreen M A Okba, V Stalin Raj, Bart L Haagmans
Middle East respiratory syndrome coronavirus (MERS-CoV) still causes outbreaks despite public awareness and implementation of health care measures, such as rapid viral diagnosis and patient quarantine. Here we describe the current epidemiological picture of MERS-CoV, focusing on humans and animals affected by this virus and propose specific intervention strategies that would be appropriate to control MERS-CoV. One-third of MERS-CoV patients develop severe lower respiratory tract infection and succumb to a fatal outcome; these patients would require effective therapeutic antiviral therapy...
June 2017: One Health
https://www.readbyqxmd.com/read/28615873/molecular-characterization-of-internal-transcribed-spacer-1-its-1-region-of-different-trypanosoma-evansi-isolates-of-india
#7
Souti Prasad Sarkhel, Surender Kumar Gupta, Jyoti Kaushik, Jarnail Singh, Deepak Kumar Gaur, Sanjay Kumar, Rajender Kumar
Six Trypanosoma evansi isolates collected from ponies (PH1 and PK6), camel (CB2), donkeys (DJ3 and DH4) and cattle (CK5) from Haryana, Rajasthan, Uttar Pradesh and Gujarat states of India were used for molecular characterization of internal transcribed spacer 1 (ITS 1). The DNA was isolated from purified trypanosomes of these six isolates after propagation in mice model. ITS1-PCR of purified parasite DNA yielded an amplification product approximately 540 bp in size. Nucleotide sequence of ITS1 gene of CB2 isolate had 530 bp while CK5, DH4, DJ3, and PH1 isolates had 532 bp, whereas, PK6 isolates had 533 bp size...
June 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28613145/two-novel-dromedary-camel-bocaparvoviruses-from-dromedaries-in-the-middle-east-with-unique-genomic-features
#8
Patrick C Y Woo, Susanna K P Lau, Hoi-Wah Tsoi, Nissy Georgy Patteril, Hazel C Yeung, Sunitha Joseph, Emily Y M Wong, Rubeena Muhammed, Franklin W N Chow, Ulrich Wernery, Kwok-Yung Yuen
The recent emergence of Middle East respiratory syndrome (MERS) coronavirus and its discovery from dromedary camels has boosted interest in the search for novel viruses in dromedaries. While bocaparvoviruses are known to infect various animals, it was not known that they exist in dromedaries. In this study, we describe the discovery of two novel dromedary camel bocaparvoviruses (DBoVs), DBoV1 and DBoV2, from dromedary faecal samples in Dubai. Among 667 adult dromedaries and 72 dromedary calves, 13.9 % of adult dromedaries and 33...
June 14, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28611730/exploiting-nanobodies-in-the-detection-and-quantification-of-human-growth-hormone-via-phage-sandwich-enzyme-linked-immunosorbent-assay
#9
Hossam Murad, Jana Mir Assaad, Rasha Al-Shemali, Abdul Qader Abbady
BACKGROUND: Monitoring blood levels of human growth hormone (hGH) in most children with short stature deficiencies is crucial for taking a decision of treatment with extended course of daily and expensive doses of recombinant hGH (rhGH or Somatropin(®)). Besides, misusing of rhGH by sportsmen is banned by the World Anti-Doping Agency and thus sensitive GH-detecting methods are highly welcome in this field. Nanobodies are the tiniest antigen-binding entity derived from camel heavy chain antibodies...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28598584/camels-in-silico-prediction-of-calmodulin-binding-proteins-and-their-binding-sites
#10
Wajid Arshad Abbasi, Amina Asif, Saiqa Andleeb, Fayyaz Ul Amir Afsar Minhas
Due to Ca(2+) -dependent binding and the sequence diversity of Calmodulin (CaM) binding proteins, identifying CaM interactions and binding sites in the wet-lab is tedious and costly. Therefore, computational methods for this purpose are crucial to the design of such wet-lab experiments. We present an algorithm suite called CaMELS (CalModulin intEraction Learning System) for predicting proteins that interact with CaM as well as their binding sites using sequence information alone. CaMELS offers state of the art accuracy for both CaM interaction and binding site prediction and can aid biologists in studying CaM binding proteins...
June 9, 2017: Proteins
https://www.readbyqxmd.com/read/28579232/chadox1-and-mva-based-vaccine-candidates-against-mers-cov-elicit-neutralising-antibodies-and-cellular-immune-responses-in-mice
#11
Naif Khalaf Alharbi, Eriko Padron-Regalado, Craig P Thompson, Alexandra Kupke, Daniel Wells, Megan A Sloan, Keith Grehan, Nigel Temperton, Teresa Lambe, George Warimwe, Stephan Becker, Adrian V S Hill, Sarah C Gilbert
The Middle East respiratory syndrome coronavirus (MERS-CoV) has infected more than 1900 humans, since 2012. The syndrome ranges from asymptomatic and mild cases to severe pneumonia and death. The virus is believed to be circulating in dromedary camels without notable symptoms since the 1980s. Therefore, dromedary camels are considered the only animal source of infection. Neither antiviral drugs nor vaccines are approved for veterinary or medical use despite active research on this area. Here, we developed four vaccine candidates against MERS-CoV based on ChAdOx1 and MVA viral vectors, two candidates per vector...
June 1, 2017: Vaccine
https://www.readbyqxmd.com/read/28578031/biophysical-evaluation-of-milk-clotting-enzymes-processed-by-high-pressure
#12
Bruno Ricardo de Castro Leite Júnior, Alline Artigiani Lima Tribst, Nicholas J Grant, Rickey Y Yada, Marcelo Cristianini
High pressure processing (HPP) is able to promote changes in enzymes structure. This study evaluated the effect of HP on the structural changes in milk-clotting enzymes processed under activation conditions for recombinant camel chymosin (212MPa/5min/10°C), calf rennet (280MPa/20min/25°C), bovine rennet (222MPa/5min/23°C), and porcine pepsin (50MPa/5min/20°C) and under inactivation conditions for all enzymes (600MPa/10min/25°C) including the protease from Rhizomucor miehei. In general, it was found that the HPP at activation conditions was able to increase the intrinsic fluorescence of samples with high pepsin concentration (porcine pepsin and bovine rennet), increase significantly the surface hydrophobicity and induce changes in secondary structure of all enzymes...
July 2017: Food Research International
https://www.readbyqxmd.com/read/28573240/signaling-pathways-regulated-by-brassicaceae-extract-inhibit-the-formation-of-advanced-glycated-end-products-in-rat-brain
#13
Abdulrahman L Al-Malki, Elie K Barbour, Huwait Ea, Said S Moselhy, Anas Hassan Saeed ALZahrani, Taha A Kumosani
BACKGROUND: The goal of this study was identification signaling molecules mediated the formation of AGEs in brain of rats injected with CdCl2 and the role of camel whey proteins and Brassicaceae extract on formation of AGEs in brain. METHODS: Ninety male rats were randomly grouped into five groups; Normal control (GpI) and the other rats (groups II-V) were received a single dose of cadmium chloride i.p (5 μg/kg/b.w) for induction of neurodegeneration. Rats in groups III-V were treated daily with whey protein (1g/kg b...
2017: African Journal of Traditional, Complementary, and Alternative Medicines: AJTCAM
https://www.readbyqxmd.com/read/28572793/bacteriocin-producing-enterococcus-faecium-lcw-44-a-high-potential-probiotic-candidate-from-raw-camel-milk
#14
Allison Vimont, Benoît Fernandez, Riadh Hammami, Ahlem Ababsa, Hocine Daba, Ismaïl Fliss
Bacterial isolates from raw camel milk were screened for antibacterial activity using the agar diffusion assay. Ten isolates selected for their inhibition of Gram-positive bacteria were identified by 16S sequencing as Enterococcus faecium or durans. An isolate named E. faecium LCW 44 exhibited the broadest antibacterial spectrum with an inhibitory activity against several Gram-positive strains belonging to the genera Clostridium, Listeria, Staphylococcus, and Lactobacillus. E. faecium LCW 44 was shown to produce N-formylated enterocins L50A and L50B, as revealed by mass spectrometry and PCR analyses...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28571958/a-decade-of-a-quick-diagnosis-unit
#15
Joaquim Torné Cachot, José Manuel Baucells Azcona, Helena Camell Ilari
No abstract text is available yet for this article.
May 29, 2017: Medicina Clínica
https://www.readbyqxmd.com/read/28562286/nutritional-and-dietary-interventions-for-autism-spectrum-disorder-a-systematic-review
#16
REVIEW
Nila Sathe, Jeffrey C Andrews, Melissa L McPheeters, Zachary E Warren
CONTEXT: Children with autism spectrum disorder (ASD) frequently use special diets or receive nutritional supplements to treat ASD symptoms. OBJECTIVES: Our objective was to evaluate the effectiveness and safety of dietary interventions or nutritional supplements in ASD. DATA SOURCES: Databases, including Medline and PsycINFO. STUDY SELECTION: Two investigators independently screened studies against predetermined criteria...
May 26, 2017: Pediatrics
https://www.readbyqxmd.com/read/28560047/effects-of-dexamethasone-on-progesterone-and-estrogen-profiles-and-uterine-progesterone-receptor-localization-during-pregnancy-in-sahel-goat-in-semi-arid-region
#17
Dauda Yahi, Nicholas Adetayo Ojo, Gideon Dauda Mshelia
BACKGROUND: Despite the widespread use of dexamethasone in veterinary and human medicine, it is reported to cause some severe pregnancy related side effects like abortion in some animals. The mechanism of the response is not clear but seems to be related to interspecies and/or breed difference in response which may involve alterations in the concentrations of some reproductive hormones. METHODS: Twenty Sahel goats comprising 18 does and 2 bucks were used for this study...
2017: Journal of Animal Science and Technology
https://www.readbyqxmd.com/read/28559621/physico-chemical-microstructural-and-rheological-properties-of-camel-milk-yogurt-as-enhanced-by-microbial-transglutaminase
#18
Nagwa H I Abou-Soliman, Sally S Sakr, Sameh Awad
Camel milk produces watery texture when it is processed to yogurt. Despite the extensive studies on microbial transglutaminase (MTGase) in dairy research, the effect of this enzyme on the properties of yogurt made from camel milk has not been studied. This study aims to investigate the impact of MTGase with and without bovine skimmed milk powder (SMP), whey protein concentrate (WPC),or β-lactoglobulin (β-lg) on physico-chemical, rheological, microstructural, and sensory properties of camel-milk yogurt during 15 days of storage period...
May 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28545987/echography-of-clinically-relevant-disorders-in-the-genital-tract-of-female-dromedary-camels
#19
Ahmed Ali, Derar Derar, Ali Alsamri, Fahd Al Sobayil
The aim of this study was to characterize the clinically relevant genital tract disorders of dromedary camels. Reproductive tract examinations were performed via transrectal palpation, ultrasonography and vaginal exploration. The ultrasonic appearance of the reproductive pathology was described and compared with its morphology at laparotomy, after surgical removal, during postmortem examination or upon slaughter. Diagnosis was also confirmed by histopathology. The most frequently encountered follicular structures were larger than typical follicles (56/338, 16...
May 19, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28545049/first-cloned-bactrian-camel-camelus-bactrianus-calf-produced-by-interspecies-somatic-cell-nuclear-transfer-a-step-towards-preserving-the-critically-endangered-wild-bactrian-camels
#20
Nisar Ahmad Wani, Binoy S Vettical, Seung B Hong
Studies were conducted to explore the possibility of employing dromedary camel (Camelus dromedarius) oocytes as recipient cytoplasts for the development of interspecies somatic cell nuclear transfer (iSCNT) embryos using skin fibroblast cells of an adult Bactrian camel (Camelus bactrianus) and Llama (Llama glama) as donor nuclei. Also, the embryos reconstructed with Bactrian cells were transferred into the uterus of synchronized dromedary camel recipients to explore the possibility of using them as surrogate mothers...
2017: PloS One
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