Ran Zhang, Lan Huang, Xiaohan Zhang, Yuanling Yu, Te Liang, Hongye Wang, Xiaomei Zhang, Di Hu, Bingwei Wang, Youchun Wang, Junyi Jiang, Xiaobo Yu
The ongoing evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to the emergence of different variants of concerns with immune evasion that have been prevalent over the past three years. Nanobodies, the functional variable regions of camelid heavy-chain-only antibodies, have garnered interest in developing neutralizing antibodies due to their smaller size, structural stability, ease of production, high affinity, and low immunogenicity, among other characteristics. In this work, we describe an integrated proteomics platform for the high-throughput screening of nanobodies against different SARS-CoV-2 spike variants...
April 11, 2024: Journal of Proteome Research