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https://www.readbyqxmd.com/read/28346045/a-modular-and-adaptive-mass-spectrometry-based-platform-for-support-of-bioprocess-development-toward-optimal-host-cell-protein-clearance
#1
Donald E Walker, Feng Yang, Joseph Carver, Koman Joe, David A Michels, X Christopher Yu
A modular and adaptive mass spectrometry (MS)-based platform was developed to provide fast, robust and sensitive host cell protein (HCP) analytics to support process development. This platform relies on one-dimensional ultra-high performance liquid chromatography (1D UHPLC) combined with several different MS data acquisition strategies to meet the needs of purification process development. The workflow was designed to allow HCP composition and quantitation for up to 20 samples per day, a throughput considered essential for real time bioprocess development support...
March 27, 2017: MAbs
https://www.readbyqxmd.com/read/28328863/surgical-outcomes-of-laparoscopic-versus-open-liver-resection-for-hepatocellular-carcinoma-for-various-resection-extent
#2
Junhua Chen, Hongyu Li, Fei Liu, Bo Li, Yonggang Wei
Although the number of laparoscopic liver resections (LRRs) has increased, studies of surgical outcomes in comparison with the conventional open approach are limited. The purpose of this study was to analyze the surgical outcomes (safety and efficacy) of LLR versus open liver resection (OLR) for hepatocellular carcinoma (HCC).We collected data on all patients who received liver resection for HCC between April 2015 and September 2016 in our institution, and retrospectively investigated the demographic and perioperative data, and also surgical outcomes...
March 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28263771/meal-pattern-alterations-associated-with-intermittent-fasting-for-weight-loss-are-normalized-after-high-fat-diet-re-feeding
#3
Juliet D Gotthardt, Nicholas T Bello
Alternate day, intermittent fasting (IMF) can be an effective weight loss strategy. However, the effects of IMF on eating behaviors are not well characterized. We investigated the acute and residual effects of IMF for weight loss on meal patterns in adult obese male C57BL/6 mice. After 8weeks of ad libitum high-fat diet to induce diet-induced obesity (DIO), mice were either continued on ad libitum high-fat diet (HFD) or placed on one of 5 diet strategies for weight loss: IMF of high-fat diet (IMF-HFD), pair-fed to IMF-HFD group (PF-HFD), ad libitum low-fat diet (LFD), IMF of low-fat diet (IMF-LFD), or pair-fed to IMF-LFD group (PF-LFD)...
March 3, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/28259469/microbial-enhanced-lindane-removal-by-sugarcane-saccharum-officinarum-in-doped-soil-applications-in-phytoremediation-and-bioaugmentation
#4
Jaseetha Abdul Salam, Mohammed A A Hatha, Nilanjana Das
The aim of this study was to examine the effect of lindane-degrading yeast on the growth and lindane uptake by Saccharum sp., in doped garden soils. The rhizosphere of Saccharum plant was amended with yeast Candida VITJzN04 by root-inoculation. The bio-augment yeast was applied in two different forms viz., planktonic form and cells immobilized on sugarcane-bagasse, in the pot experiments. Garden soils (lindane∼100 mg/kg) exposed to various treatments were monitored for a period of 30 days, for residual lindane by gas-chromatography analysis...
May 15, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28258383/-new-worldwide-guidelines-for-treatment-of-inguinal-hernia-the-most-important-recommendations-from-herniasurge
#5
D Weyhe
SURGICAL TECHNIQUES: In the HerniaSurge guidelines the highest evidence is found with respect to recommendations for mesh-based surgery techniques. This includes Lichtenstein as well as the minimally invasive TEP/TAPP surgery. For discreet symptomatic or asymptomatic inguinal hernia, watchful waiting is an option if the state of health and social situation are taken into account; however, femoral hernia should be treated promptly with a mesh technique, with laparoendoscopic techniques being the more favored option...
March 3, 2017: Der Chirurg; Zeitschrift Für Alle Gebiete der Operativen Medizen
https://www.readbyqxmd.com/read/28257664/intake-of-total-saponins-and-polysaccharides-from-polygonatum-kingianum-affects-the-gut-microbiota-in-diabetic-rats
#6
Hongli Yan, Jianmei Lu, Yanfang Wang, Wen Gu, Xingxin Yang, Jie Yu
BACKGROUND: The gut microbiota has been reported to play a critical role in metabolic diseases, including in diabetes. Polygonatum kingianum has been used in the treatment of diabetes and related diseases in China for centuries. Total saponins (TSPK) and total polysaccharides (PSPK) were reported to be major types of active constituents of P. kingianum. This research aims at investigation of their therapeutical mechanisms on diabetes based on the regulation of gut microbiota. STUDY DESIGN: Type 2 diabetes (T2D) rats were induced by high-fat diet (HFD) and streptozotocin-injection from male Sprague-Dawley (SD) rats...
March 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28255839/impact-of-dehydration-and-fasting-on-intraocular-pressure-and-corneal-biomechanics-measured-by-the-ocular-response-analyzer
#7
Betul Seher Uysal, Necati Duru, Umut Ozen, Mucella Arikan Yorgun, Emine Akcay, Mehtap Caglayan, Nurullah Cagil
PURPOSE: To investigate the effects of dehydration and fasting on the intraocular pressure (IOP) and corneal biomechanics during Ramadan in healthy subjects. METHODS: A total of 36 healthy fasting male volunteers with a mean age of 32.7 ± 5.1 years (range 28-38 years) were enrolled in the study. A Reichert Ocular Response Analyzer (ORA) was used to measure the corneal resistance factor (CRF), corneal hysteresis (CH), Goldman-correlated IOP (IOPg), and corneal-compensated IOP (IOPcc), additionally IOP with Goldmann applanation tonometer (IOP-GAT) was taken...
March 2, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28229567/repeated-sprint-training-in-the-fasted-state-during-ramadan-morning-or-evening-training
#8
Asma Aloui, Tarak Driss, Hana Baklouti, Hamdi Jaafar, Omar Hammouda, Karim Chamari, Nizar Souissi
BACKGROUND: The present study assessed the optimal moment-of-day for repeated-sprint training in the fasted state during Ramadan. METHODS: Thirty amateur soccer players were randomly assigned to a morning training group (MTG, training at ~08:00 a.m., n = 10), an evening training group (ETG, training at ~06:00 p.m., n = 10), and a control group (n = 10). Training sessions, conducted on alternate days, consisted of 3 sets of 6 × 40-m shuttle sprints (2 × 20 m with 180° direction changes)...
February 22, 2017: Journal of Sports Medicine and Physical Fitness
https://www.readbyqxmd.com/read/28225054/nutritional-adequacy-of-diets-for-adolescents-with-overweight-and-obesity-considerations-for-dietetic-practice
#9
N B Lister, M L Gow, K Chisholm, A Grunseit, S P Garnett, L A Baur
BACKGROUND/OBJECTIVES: Adolescents have unique nutrient requirements due to rapid growth and development. High rates of obesity in adolescents require a variety of diet interventions to achieve weight loss under clinical supervision. The aim of this study is to examine the nutritional adequacy of energy-restricted diets for adolescents. SUBJECTS/METHODS: Three popular diets were modelled for 7 days and assessed by comparing the nutrient profile to the Australian Nutrient Reference Values...
February 22, 2017: European Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28212832/motor-dysfunction-and-alterations-in-glutathione-concentration-cholinesterase-activity-and-bdnf-expression-in-substantia-nigra-pars-compacta-in-rats-with-pedunculopontine-lesion
#10
Lisette Blanco-Lezcano, Javier Jimenez-Martin, Mei-Li Díaz-Hung, Esteban Alberti-Amador, Maylin Wong-Guerra, Ma Elena González-Fraguela, Bárbara Estupiñán-Díaz, Teresa Serrano-Sánchez, Liliana Francis-Turner, Susana Delgado-Ocaña, Yanier Núñez-Figueredo, Yamilé Vega-Hurtado, Isabel Fernández-Jiménez
Pedunculopontine nucleus (PPN) has been considered a critically important region in the regulation of some of the physiological functions that fail during the progression of Parkinson's disease (PD). In this paper, the effects of unilateral neurotoxic lesion of the PPN [through the injection of N-methyl-d-aspartate (NMDA) solution (concentration: 0.1M; volume: 0.5µL)] in motor execution and gait disorders and the changes in cellular and molecular indicators in rat nigral tissue were evaluated. The motor execution was assessed using the beam test (BT) and the gait disorders by footprint test...
February 15, 2017: Neuroscience
https://www.readbyqxmd.com/read/28193647/piperaquine-population-pharmacokinetics-and-cardiac-safety-in-cambodia
#11
Pattaraporn Vanachayangkul, Chanthap Lon, Michele Spring, Sommethy Sok, Winita Ta-Aksorn, Chanikarn Kodchakorn, Sut-Thang Pann, Soklyda Chann, Mali Ittiverakul, Sabaithip Sriwichai, Nillawan Buathong, Worachet Kuntawunginn, Mary So, Theng Youdaline, Erin Milner, Mariusz Wojnarski, Charlotte Lanteri, Jessica Manning, Satharath Prom, Mark Haigney, Louis Cantilena, David Saunders
Despite rising resistance, dihydroartemisinin-piperaquine (DP) remains a first line therapy for uncomplicated malaria in many parts of Cambodia. While generally well-tolerated as a 3-day regimen, compressed 2-day regimens were associated with treatment-limiting cardiac repolarization effects in a recent clinical trial. To better estimate the risks of piperaquine concentration on QT interval prolongation, we pooled data from 3 randomized clinical trials between 2010 and 2014 in northern Cambodia. A population pharmacokinetic model was developed to compare exposure-response relationships between 2-day (2DP) and 3-day (3DP) regimens while accounting for differences in regimen and sample collection times between studies...
February 13, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28192975/third-harmonic-scattering-for-fast-and-sensitive-screening-of-the-second-hyperpolarizability-in-solution
#12
Nick Van Steerteghem, Koen Clays, Thierry Verbiest, Stijn Van Cleuvenbergen
Organic materials are promising candidates for integration in optical network components allowing fast communication. Ultimate speeds can be obtained by exploiting third-order nonlinear optical light-matter interactions that ultimately rely on the molecular second hyperpolarizability (γ). The exploration of molecular structure-property relations is crucial to optimize γ but requires state of the art measurement techniques which are both sensitive and efficient. Unfortunately, present-day methods for probing the performance of third-order nonlinear optical (NLO) materials fail to meet at least one of those requirements...
February 15, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28177396/growth-heterogeneity-in-broiler-breeder-pullets-is-settled-before-the-onset-of-feed-restriction-but-is-not-predicted-by-size-at-hatch
#13
C Lindholm, J Jönsson, A Calais, A Middelkoop, N Yngwe, E Berndtson, J J Lees, E Hult, J Altimiras
Uniform growth is a desirable trait in all large-scale animal production systems because it simplifies animal management and increases profitability. In parental broiler flocks, so-called broiler breeders, low growth uniformity is largely attributed to the feed competition that arises from quantitatively restricted feeding. As feed restriction is crucial to maintaining healthy and fertile breeders, several practices for reducing feed competition and the associated growth heterogeneity have been suggested and range from nutrient dilution by increasing fiber content in feed to intermittent fasting with increased portion size ("skip a day"), but no practice appears to be entirely effective...
January 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28146063/consuming-two-eggs-per-day-as-compared-to-an-oatmeal-breakfast-decreases-plasma-ghrelin-while-maintaining-the-ldl-hdl-ratio
#14
Amanda Missimer, Diana M DiMarco, Catherine J Andersen, Ana Gabriela Murillo, Marcela Vergara-Jimenez, Maria Luz Fernandez
Eggs contain high quality protein, vitamins, minerals and antioxidants, yet regular consumption is still met with uncertainty. Therefore, the purpose of this study was to compare the effects of consuming two eggs per day or a heart-healthy oatmeal breakfast on biomarkers of cardiovascular disease (CVD) risk and satiety measures in a young, healthy population. Fifty subjects participated in a randomized crossover clinical intervention; subjects were randomly allocated to consume either two eggs or one packet of oatmeal per day for breakfast for four weeks...
January 29, 2017: Nutrients
https://www.readbyqxmd.com/read/28138096/ptf1a-ela3l-cells-are-developmentally-maintained-progenitors-for-exocrine-regeneration-following-extreme-loss-of-acinar-cells-in-zebrafish-larvae
#15
Nicole Schmitner, Kenji Kohno, Dirk Meyer
The exocrine pancreas displays a significant capacity for regeneration and renewal. In humans and mammalian model systems, the partial loss of exocrine tissue, such as after acute pancreatitis or partial pancreatectomy induces rapid recovery via expansion of surviving acinar cells. In mouse it was further found that an almost complete removal of acinar cells initiates regeneration from a currently not well-defined progenitor pool. Here we used the zebrafish as an alternative model to study cellular mechanisms of exocrine regeneration following an almost complete removal of acinar cells...
January 30, 2017: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/28115622/robust-and-rapid-algorithms-facilitate-large-scale-whole-genome-sequencing-downstream-analysis-in-an-integrative-framework
#16
Miaoxin Li, Jiang Li, Mulin Jun Li, Zhicheng Pan, Jacob Shujui Hsu, Dajiang J Liu, Xiaowei Zhan, Junwen Wang, Youqiang Song, Pak Chung Sham
Whole genome sequencing (WGS) is a promising strategy to unravel variants or genes responsible for human diseases and traits. However, there is a lack of robust platforms for a comprehensive downstream analysis. In the present study, we first proposed three novel algorithms, sequence gap-filled gene feature annotation, bit-block encoded genotypes and sectional fast access to text lines to address three fundamental problems. The three algorithms then formed the infrastructure of a robust parallel computing framework, KGGSeq, for integrating downstream analysis functions for whole genome sequencing data...
January 23, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28115614/feedback-traps-for-virtual-potentials
#17
REVIEW
Momčilo Gavrilov, John Bechhoefer
Feedback traps are tools for trapping and manipulating single charged objects, such as molecules in solution. An alternative to optical tweezers and other single-molecule techniques, they use feedback to counteract the Brownian motion of a molecule of interest. The trap first acquires information about a molecule's position and then applies an electric feedback force to move the molecule. Since electric forces are stronger than optical forces at small scales, feedback traps are the best way to trap single molecules without 'touching' them (e...
March 6, 2017: Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
https://www.readbyqxmd.com/read/28109417/associations-of-cooking-with-dietary-intake-and-obesity-among-supplemental-nutrition-assistance-program-participants
#18
Lindsey Smith Taillie, Jennifer M Poti
INTRODUCTION: Participation in the Supplemental Nutrition Assistance Program (SNAP) may help ease economic and time constraints of cooking, helping low-income households prepare healthier meals. Therefore, frequent cooking may be more strongly associated with improved dietary outcomes among SNAP recipients than among income-eligible non-recipients. Alternately, increased frequency of home-cooked meals among SNAP participants may be beneficial simply by replacing fast food intake. This study quantified the association between home cooking and fast food with diet intake and weight status among SNAP recipients...
February 2017: American Journal of Preventive Medicine
https://www.readbyqxmd.com/read/28107043/the-impact-of-meal-timing-on-cardiometabolic-syndrome-indicators-in-shift-workers
#19
Hylton E Molzof, Michael D Wirth, James B Burch, Nitin Shivappa, James R Hebert, Russell L Johnson, Karen L Gamble
The aims of this study were to 1) compare the inflammatory potential of night- and day-shift nurses' diets with regard to time of day and work status and 2) explore how the timing of food intake during work and off-work is associated with cardiometabolic syndrome (CMS) risk factors between these two groups. Female nurses (N = 17; 8 day-shift and 9 night-shift) reported food intake over 9 days. On a middle day off of work, metabolic parameters were measured after an overnight fast. Energy/macronutrient intake and inflammatory potential of dietary intake (as assessed via the Dietary Inflammatory Index(TM)) were calculated for nurses' workdays, work nights, off-work days, and off-work nights...
2017: Chronobiology International
https://www.readbyqxmd.com/read/28094665/fdg-pet-reproducibility-in-tumor-bearing-mice-comparing-a-traditional-suv-approach-with-a-tumor-to-brain-tissue-ratio-approach
#20
Morten Busk, Ole L Munk, Steen Jakobsen, Jørgen Frøkiær, Jens Overgaard, Michael R Horsman
BACKGROUND: Current [F-18]-fluorodeoxyglucose positron emission tomography (FDG-PET) procedures in tumor-bearing mice typically includes fasting, anesthesia, and standardized uptake value (SUV)-based quantification. Such procedures may be inappropriate for prolonged multiscan experiments. We hypothesize that normalization of tumor FDG retention relative to a suitable reference tissue may improve accuracy as this method may be less susceptible to uncontrollable day-to-day changes in blood glucose levels, physical activity, or unnoticed imperfect tail vein injections...
January 17, 2017: Acta Oncologica
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