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https://www.readbyqxmd.com/read/29668133/helminth-eggs-as-parasitic-indicators-of-fecal-contamination-in-agricultural-irrigation-water-biosolids-soils-and-pastures
#1
María Claudia Campos, Milena Beltrán, Nancy Fuentes, Gerardo Moreno
INTRODUCTION: A very common practice in agriculture is the disposal of wastewater and biosolids from water treatment systems due to their high nutrient content, which substantially improves crop yields. However, the presence of pathogens of fecal origin creates a sanitary risk to farmers and consumers. OBJECTIVE: To determine the presence and concentration of helminth eggs in irrigation waters, biosolids, agricultural soils, and pastures. MATERIALS AND METHODS: Water, biosolids, soil, and pasture samples were collected and analyzed for helminth egg detection, total eggs and viable eggs counts...
March 15, 2018: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/29668114/-%C3%AE-2-adrenoceptors-inhibit-neutrophil-extracellular-traps-in-human-polymorphonuclear-leukocytes
#2
Franca Marino, Angela Scanzano, Laura Pulze, Monica Pinoli, Emanuela Rasini, Alessandra Luini, Raffaella Bombelli, Massimiliano Legnaro, Magda de Eguileor, Marco Cosentino
This study tests the hypothesis that in isolated human polymorphonuclear leukocytes (PMN) adrenergic ligands can affect neutrophil extracellular trap (NET) formation. We have previously shown that, in PMN, adrenaline (A), through the activation of adrenergic receptors (AR), reduces stimulus-dependent cell activation; we have, therefore, planned to investigate if AR are involved in NET production. PMN were obtained from venous blood of healthy subject. The ability of adrenergic ligands to affect reactive oxygen species (ROS) production, NET production, and cell migration was investigated in cells cultured under resting conditions or after activation with N-formyl-methionyl-leucyl-phenylalanine (fMLP), LPS, or IL-8...
April 18, 2018: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/29668078/adherence-and-immune-response-to-revaccination-following-hematopoietic-stem-cell-transplantation-at-a-pediatric-onco-hematology-reference-center
#3
Fernanda Gouveia-Alves, Roseane Gouveia, Valéria C Ginani, Adriana Seber, Danielle A Kuramoto, Gabriel F A Murad, Fernanda G Spina, Antonio S Petrilli, Victor G Zecchin, Celso Granato, Fabianne Carlesse, M Isabel de Moraes-Pinto
BACKGROUND: Revaccination after hematopoietic stem cell transplantation (HSCT) is necessary to compensate for the loss of immunological memory. The aims of this study were to evaluate the adherence to revaccination schedule and the humoral immune response to different vaccine antigens in HSCT pediatric and young adult patients. METHODS: Patients submitted to HSCT for over 3 years were recruited. After written informed consent, a questionnaire was filled in, the vaccination card was analyzed, a blood sample was collected and tested by ELISA for diphtheria, Haemophilus influenzae type b (Hib), hepatitis A, hepatitis B, tetanus, measles, rubella and varicella antibodies...
April 18, 2018: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/29668077/jc-and-bk-virus-dna-detection-in-archival-slides-of-urine-cytospin-from-renal-transplant-patients
#4
Patricia Assis, Carlos Eduardo Carvalho, Marcelo Soares Silva, Bruna Ribeiro, Maria da Gloria Carvalho
BACKGROUND: Although identifying cytological viral inclusions (decoy cells) in the urine is relatively easy, distinguishing between Polyomaviruses BKV and JCV is not possible. Few studies have been published regarding JCV detection in kidney transplant recipients. OBJECTIVE: To evaluate the incidence of BKV and JCV DNA in archival slides of urine cytospin material from renal transplant patients. METHODS: A total of 44 urine specimens were evaluated cytologically for the presence of viral inclusions (decoy cells) and by nested polymerase chain reaction to differentiate between JCV and BKV in DNA isolated from archival slides of urine cytospin material...
April 18, 2018: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/29668074/a-20-year-experience-with-nocardiosis-in-solid-organ-transplant-sot-recipients-in-the-southwestern-united-states-a-single-center-study
#5
Aneela Majeed, Norman Beatty, Ahmad Iftikhar, Adeela Mushtaq, Julia Fisher, Pryce Gaynor, Jeeyong C Kim, Jose Luis Marquez, Francisco E Mora, Anca Georgescu, Tirdad Zangeneh
BACKGROUND: Nocardiosis is a life-threatening opportunistic infection. Solid organ transplant (SOT) recipients are at higher risk (incidence 0.04% to 3.5%) of developing nocardiosis. Rate of nocardiosis in the Southwestern US may be high due to environmental factors. METHODS: We performed a retrospective review study on 54 SOT patients diagnosed with Nocardia between 1997 and 2016 at our center. To explore the association of various risk factors with both the development of disseminated disease and mortality, a series of Fisher's exact tests was used...
April 18, 2018: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/29668071/human-papillomavirus-an-unlikely-etiologic-factor-in-sinonasal-inverted-papilloma
#6
Sepideh Mohajeri, Chi Lai, Bibianna Purgina, Dakheelallah Almutairi, Tabassom Baghai, Jim Dimitroulakos, Shaun Kilty
OBJECTIVES/HYPOTHESIS: Tenuous evidence has supported the hypothesis that sinonasal inverted papilloma (SNIP) arise from human papillomavirus (HPV) infection. To clarify the role of HPV in SNIP, all known HPV sub-types were evaluated by employing a robust polymerase chain reaction-based method in a wide variety of SNIPs from a single institution. STUDY DESIGN: Retrospective surgical specimen tumor sample analysis. METHODS: HPV positivity among SNIP samples and those with squamous cell carcinoma (SCC) were compared...
April 18, 2018: Laryngoscope
https://www.readbyqxmd.com/read/29668062/long-term-outcomes-and-molecular-analysis-of-a-large-cohort-of-patients-with-46-xy-disorder-of-sex-development-due-to-partial-gonadal-dysgenesis
#7
Nathalia Lisboa Gomes, Antônio Marcondes Lerário, Aline Machado Zamboni, Daniela Rodrigues de Moraes, Thatiana Evilen da Silva, Ivo Jorge Prado Arnhold, Rafael Loch Batista, José Antônio Diniz Faria Júnior, Elaine F Costa, Mirian Y Nishi, Marlene Inacio, Sorahia Domenice, Berenice B Mendonca
BACKGROUND: Follow-up data on patients with 46,XY partial gonadal dysgenesis (PGD) until adulthood are scarce, making information on prognosis difficult. OBJECTIVE: To analyse the long-term outcomes of patients with 46,XY PGD regarding testosterone production, germ cell tumour risk, genotype, and psychosexual adaptation. METHODS: A retrospective longitudinal study of 33 patients (20 assigned male and 13 patients assigned female at birth). Molecular diagnosis was performed by Sanger sequencing or by targeted massively parallel sequencing of 63 genes related to disorders of sex development (DSDs)...
April 18, 2018: Clinical Endocrinology
https://www.readbyqxmd.com/read/29668047/miniaturization-of-the-microsuspension-salmonella-microsome-assay-in-agar-microplates
#8
José Ricardo R M Zwarg, Daniel A Morales, Bianca S Maselli, Werner Brack, Gisela A Umbuzeiro
The Salmonella/microsome assay (Ames test) is the most widely used mutagenicity test for the evaluation of pure chemicals and environmental samples. There are several versions of protocols available in the literature, including those that reduce the amount of sample needed for testing with liquid and agar media. The microsuspension version of the Salmonella/microsome assay is more sensitive than the standard protocol. It is performed using 5-times concentrated bacteria and less sample and S9 mixture, but still uses conventional Petri dishes (90 × 15 mm)...
April 18, 2018: Environmental and Molecular Mutagenesis
https://www.readbyqxmd.com/read/29668045/when-does-the-influence-of-maturation-on-anthropometric-and-physical-fitness-characteristics-increase-and-subside
#9
Christopher Towlson, Stephen Cobley, Guy Parkin, Ric Lovell
The relationships between maturation and anthropometric and physical performance characteristics are dynamic and often asynchronous; confounding the capability to accurately evaluate performance during adolescence. This study aimed to (i) examine the influence of chronological age (CA) and somatic maturation (YPHV) upon anthropometric and physical performance parameters, and (ii) identify the transition/change time-points in these relationships using segmental regression. N=969 soccer players (8-18 years of age) completed anthropometric and physical test assessments, including a counter-movement jump (CMJ), agility T-test, 10 and 20m sprints, and multi-stage fitness test (MSFT)...
April 18, 2018: Scandinavian Journal of Medicine & Science in Sports
https://www.readbyqxmd.com/read/29668036/optimization-of-extraction-of-hypoglycemic-ingredients-from-grape-seeds-and-evaluation-of-%C3%AE-glucosidase-and-%C3%AE-amylase-inhibitory-effects-in-vitro
#10
Fansheng Kong, Yaru Qin, Zhipeng Su, Zhengxiang Ning, Shujuan Yu
Grape seeds full with oil and also rich in hypoglycemic ingredients. This study is to improve the comprehensive utilization of grape seeds and the quality of grape seeds oil. Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively, are 171.1 ± 1.1, 658.8 ± 0.2, 482.3 ± 0.2 (mg GSAE/g). Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1...
April 18, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29668034/response-of-formed-biofilm-of-enterobacter-cloacae-klebsiella-oxytoca-and-citrobacter-freundii-to-chlorite-based-disinfectants
#11
Linlin Cai, Huawei Wang, Lijiao Liang, Guangyu Wang, Xinglian Xu, Huhu Wang
Bacterial biofilms formed on equipment surfaces are potential sources of cross-contamination and can be responsible for the spread of bacteria involved in food spoilage, such as some Enterobacteriaceae family members. In this study, the effect of chlorite-based disinfectants, including sodium hypochlorite (SH), chlorine dioxide (CD), strongly acidic electrolyzed water (StAEW), and neutral electrolyzed water (NEW), on inactivation of mono-biofilms of Enterobacter cloacae, Klebsiella oxytoca, and Citrobacter freundii was evaluated separately...
April 18, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29668033/sexual-dimorphism-in-postnatal-gonadotropin-levels-in-infancy-reflects-diverse-maturation-of-the-ovarian-and-testicular-hormone-synthesis
#12
Tanja Kuiri-Hänninen, Leo Dunkel, Ulla Sankilampi
BACKGROUND: The postnatal gonadotropin surge is sexually dimorphic: FSH levels predominate in girls and LH levels in boys. However, in preterm (PT) girls, both gonadotropin levels are higher than in PT boys. OBJECTIVE: To evaluate how gonadal maturation contributes to the sex differences in FSH and LH. DESIGN: Monthly follow-up of 58 full-term (FT, 29 boys) and 67 PT (33 boys) infants from one week (D7) to six months of age (M1-M6). Analyses were also done according to postmenstrual (PM) age in PT infants...
April 18, 2018: Clinical Endocrinology
https://www.readbyqxmd.com/read/29668027/heart-transplant-in-children-what-a-primary-care-provider-needs-to-know
#13
Swati Sehgal, Emily Shea, Lauren Kelm, Deepak Kamat
Heart transplantation is offered to children with heart failure that is not amenable to medical or surgical therapy. Indications for heart transplant include unrepairable congenital heart disease, failed palliation of congenital heart disease, and cardiomyopathies. There has been tremendous progress in this field since the first heart transplant was performed in 1967. Each year, approximately 500 pediatric heart transplants take place worldwide. Pediatric heart transplant survivors are living longer with their initial transplant...
April 1, 2018: Pediatric Annals
https://www.readbyqxmd.com/read/29668026/nicu-graduates-the-role-of-the-allied-health-team-in-follow-up
#14
Jane L Orton, Joy E Olsen, Katherine Ong, Rochelle Lester, Alicia J Spittle
Infants who graduate from the neonatal intensive care unit, including those infants born preterm and/or with brain injury, are at increased risk of long-term neurodevelopmental impairments. The developmental allied health team, consisting of physical therapy, occupational therapy, and speech pathology, is crucial in early evaluation of gross motor, fine motor, feeding, and language development. Surveillance of neurodevelopment in the first year of life is essential to ensure early detection of specific developmental delays and impairments, and to ensure timely referral for early intervention...
April 1, 2018: Pediatric Annals
https://www.readbyqxmd.com/read/29668018/how-strong-was-the-bottleneck-associated-to-the-peopling-of-the-americas-new-insights-from-multilocus-sequence-data
#15
Nelson J R Fagundes, Alice Tagliani-Ribeiro, Rohina Rubicz, Larissa Tarskaia, Michael H Crawford, Francisco M Salzano, Sandro L Bonatto
In spite of many genetic studies that contributed for a deep knowledge about the peopling of the Americas, no consensus has emerged about important parameters such as the effective size of the Native Americans founder population. Previous estimates based on genomic datasets may have been biased by the use of admixed individuals from Latino populations, while other recent studies using samples from Native American individuals relied on approximated analytical approaches. In this study we use resequencing data for nine independent regions in a set of Native American and Siberian individuals and a full-likelihood approach based on isolation-with-migration scenarios accounting for recent flow between Asian and Native American populations...
2018: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29668016/genetic-and-ecological-niche-modeling-of-calydorea-crocoides-iridaceae-an-endemic-species-of-subtropical-highland-grasslands
#16
Juliana Lustosa Matos de Alencar, Eliane Kaltchuk-Santos, Juliana Fachinetto, Luana Olinda Tacuatiá, Eliana Regina Forni-Martins, Eudes Maria Stiehl-Alves, Tatiana Teixeira de Souza-Chies
Evolutionary analyses have been widely used for evaluation of genetic diversity of natural populations and correlate these data to the fitness of the species, especially in the case of threatened species. Calydorea crocoides occurs in a restricted area at altitudes from 800 to 1500 m in southern Brazil and is considered endangered. A study assessing genetic diversity, cytogenetic features and ecological niche was performed aiming to characterize C. crocoides by multidisciplinary approaches. Molecular data highlighted that most of the total variation (76%; p < 0...
2018: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29667942/smoking-is-a-risk-factor-for-relapse-of-antimyeloperoxidase-antibodies-associated-vasculitis
#17
Makoto Yamaguchi, Masahiko Ando, Takayuki Katsuno, Naotake Tsuboi, Shoichi Maruyama
BACKGROUND/OBJECTIVE: Several studies have identified predictors of relapse in antineutrophil cytoplasmic antibody-associated small-vessel vasculitis. However, the role of smoking as a risk factor of relapse has not been elucidated. Therefore, this study aimed to evaluate whether a history of smoking is a dose-dependent independent risk factor for antineutrophil cytoplasmic antibody-associated small-vessel vasculitis relapse. METHODS: This multicenter retrospective cohort study included 122 patients with granulomatosis with polyangiitis and microscopic polyangiitis (MPA) from 9 nephrology centers in Japan...
April 5, 2018: Journal of Clinical Rheumatology: Practical Reports on Rheumatic & Musculoskeletal Diseases
https://www.readbyqxmd.com/read/29667937/structural-properties-of-templated-ge-quantum-dot-arrays-impact-of-growth-and-pre-pattern-parameters
#18
Jenny Tempeler, Serhiy Danylyuk, Sascha Brose, Peter Loosen, Larissa Juschkin
In this study we analyze the impact of process and growth parameters on the structural properties of germanium (Ge) quantum dot arrays. The arrays were deposited by molecular beam epitaxy on pre-patterned silicon (Si) substrates. Periodic arrays of pits with diameters between 120 nm to 20 nm and pitches ranging from 200 nm down to 40 nm were etched into the substrate prior to growth. The structural perfection of the 2-dimensional quantum dot (QD) arrays was evaluated based on SEM images. The impact of two processing steps on the directed self-assembly of Ge QD arrays is investigated...
April 18, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29667932/fused-data-transrectal-eit-for-prostate-cancer-imaging
#19
Ethan K Murphy, Xiaotian Wu, Ryan J Halter
OBJECTIVE: Prostate cancer is a significant problem affecting 1 in 7 men. Unfortunately, the diagnostic gold-standard of ultrasound-guided biopsy misses 10-30% of all cancers. The objective of this study was to develop an Electrical Impedance Tomography (EIT) approach that has the potential to image the entire prostate using multiple impedance measurements recorded between electrodes integrated onto an end-fired transrectal ultrasound (TRUS) device and a biopsy probe (BP). APPROACH: Simulations and sensitivity analyses were used to investigate the best combination of electrodes, and measured tank experiments were used to evaluate a fused-data transrectal EIT (fd-TREIT) and BP approach...
April 18, 2018: Physiological Measurement
https://www.readbyqxmd.com/read/29667926/proximal-tibia-stress-fracture-with-osteoarthritis-of-knee-radiological-and-functional-analysis-of-one-stage-tka-with-long-stem
#20
Dhanasekaran Soundarrajan, Natesan Rajkumar, Palanisamy Dhanasekararaja, Shanmuganathan Rajasekaran
INTRODUCTION: Proximal tibia stress fractures with knee osteoarthritis pose a challenging situation. We evaluated the radiological and functional outcome of one-stage total knee arthroplasty (TKA) and long stem for patients with varied grades of knee arthritis and proximal tibia stress fractures.  Methods: We analysed 20 patients from April 2012 to March 2017 with proximal tibia stress fractures associated with knee osteoarthritis of varied grades. Out of 20 patients, five were acute fresh fractures...
2018: SICOT-J
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