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https://www.readbyqxmd.com/read/28818783/development-of-a-porous-3d-graphene-pdms-scaffold-for-improved-osseointegration
#1
Jianfeng Li, Xiao Liu, Jeremy M Crook, Gordon G Wallace
Osseointegration in orthopedic surgery plays an important role for bone implantation success. Traditional treatment of implant surface aimed at improved osseointegration has limited capability for its poor performance in supporting cell growth and proliferation. Polydimethylsiloxane (PDMS) is a widely used silicon-based organic polymer material with properties that are useful in cosmetics, domestic applications and mechanical engineering. In addition, the biocompatibility of PDMS, in part due to the high solubility of oxygen, makes it an ideal material for cell-based implants...
August 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28814956/rapid-rapamycin-only-induced-osteogenic-differentiation-of-blood-derived-stem-cells-and-their-adhesion-to-natural-and-artificial-scaffolds
#2
Carpentieri Arianna, Cozzoli Eliana, Acri Flavio, Ranalli Marco, Diedenhofen Giacomo, Scimeca Manuel, Bonanno Elena, Gambacurta Alessandra
Stem cells are a centerpiece of regenerative medicine research, and the recent development of adult stem cell-based therapy systems has vigorously expanded the scope and depth of this scientific field. The regeneration of damaged and/or degraded bone tissue in orthopedic, dental, or maxillofacial surgery is one of the main areas where stem cells and their regenerative potential could be used successfully, requiring tissue engineering solutions incorporating an ideal stem cell type paired with the correct mechanical support...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28811545/sealing-the-pores-of-peo-coating-with-mg-al-layered-double-hydroxide-enhanced-corrosion-resistance-cytocompatibility-and-drug-delivery-ability
#3
Feng Peng, Donghui Wang, Yaxin Tian, Huiliang Cao, Yuqin Qiao, Xuanyong Liu
In recent years, magnesium (Mg) alloys show a promising application in clinic as degradable biomaterials. Nevertheless, the poor corrosion resistance of Mg alloys is the main obstacle to their clinical application. Here we successfully seal the pores of plasma electrolytic oxidation (PEO) coating on AZ31 with Mg-Al layered double hydroxide (LDH) via hydrothermal treatment. PEO/LDH composite coating possess a two layer structure, an inner layer made up of PEO coating (~5 μm) and an outer layer of Mg-Al LDH (~2 μm)...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28766102/a-new-chapter-for-mesenchymal-stem-cells-decellularized-extracellular-matrices
#4
REVIEW
Yagiz Anasiz, Riza Koksal Ozgul, Duygu Uckan-Cetinkaya
From orthopedic to neurological disorders, stem cells are used as platforms to understand disease mechanisms and considered as novel and promising treatment options, especially when the valid therapeutic approaches are unavailable or ineffective. There are different stem cell types in the literature, however the spindle-shaped, colony forming and multilineage-differentiating cells, also known as mesenchymal stem cells (MSC) are very popular, as MSC can be isolated from different tissues with minimal ethical concerns and without tumor formations, which make them easily accessible and widely used in vitro and in vivo studies...
August 1, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28747099/in-vivo-models-for-the-evaluation-of-the-osteogenic-potency-of-bone-substitutes-seeded-with-mesenchymal-stem-cells-of-human-origin-a-concise-review-special-issue-animal-models-in-tissue-engineering
#5
Fabian Westhauser, Anne-Sophie Senger, Bruno Reible, Arash Moghaddam
Research concerning bone substitutes is one of the most challenging fields in orthopedic research and has a high clinical relevance, especially since the currently available bone substitutes are limited in their osteostimulative capabilities. In-vitro models for the evaluation of the properties of bone substitutes allow the use of human mesenchymal stem cells (hMSC) seeded onto scaffolds, but suffer from the lack of a physiological environment for those cells. Most in-vivo models include the use of non-human MSC and are therefore lacking in clinical relevance...
July 26, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28698718/the-holy-grail-of-orthopedic-surgery-mesenchymal-stem-cells-their-current-uses-and-potential-applications
#6
REVIEW
Roberto Berebichez-Fridman, Ricardo Gómez-García, Julio Granados-Montiel, Enrique Berebichez-Fastlicht, Anell Olivos-Meza, Julio Granados, Cristina Velasquillo, Clemente Ibarra
Only select tissues and organs are able to spontaneously regenerate after disease or trauma, and this regenerative capacity diminishes over time. Human stem cell research explores therapeutic regenerative approaches to treat various conditions. Mesenchymal stem cells (MSCs) are derived from adult stem cells; they are multipotent and exert anti-inflammatory and immunomodulatory effects. They can differentiate into multiple cell types of the mesenchyme, for example, endothelial cells, osteoblasts, chondrocytes, fibroblasts, tenocytes, vascular smooth muscle cells, and sarcomere muscular cells...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28672942/adenovirus-mediated-bone-morphogenetic-protein-2-promotes-osteogenic-differentiation-in-human-mesenchymal-stem-cells-in-vitro
#7
Hong Cao, Zhi-Bo Sun, Lei Zhang, Wei Qian, Chun-Yang Li, Xiao-Peng Guo, Ying Zhang
Delayed and failed bone union following fracture is a common clinical complication that requires treatment in orthopedics. Cell-based therapies and tissue-engineering approaches are potential therapeutic strategies for bone repair and fracture healing. However, the effect of adenovirus expressing bone morphogenetic protein-2 (Ad-BMP-2) on the osteogenic ability of human mesenchymal stem cells (hMSCs) has remained to be fully elucidated. Therefore, in the present study, hMSCs were transduced using Ad-BMP-2 to assess the effects of its application and to determine whether Ad-BMP-2 promotes the osteogenic differentiation of hMSCs...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28664842/difference-between-biomarkers-of-tibial-bone-marrow-and-adipose-tissue
#8
Ersin Kuyucu, Mehmet Erdil, Adnan Kara, Murat Bülbül
BACKGROUND: Stem cells, with their regeneration capacity, long-term viability, and differentiation characteristics, have indispensable biological properties. As described by Hauner and Grigoradis et al., mesenchymal stem cell originating from adipose or bone marrow can be differentiated into many tissues such as adipocyte, chondrocyte, myeloblast, and osteoblast. The aim of our study is to compare the use of adipose and tibial bone marrow derived stem cells for therapeutic purposes in orthopedic surgery, which has not been clearly evaluated in the literature to our knowledge and to also evaluate their use...
2017: SICOT-J
https://www.readbyqxmd.com/read/28639702/bone-marrow-aspirate-clot-a-technical-complication-or-a-smart-approach-for-musculoskeletal-tissue-regeneration
#9
REVIEW
Francesca Salamanna, Deyanira Contartese, Nicolò Nicoli Aldini, Giovanni Barbanti Brodano, Cristiana Griffoni, Alessandro Gasbarrini, Milena Fini
One of the methods employed to improve healing of damaged tissues is the use of cellular based therapies. A number of regenerative medicine based strategies, from in vitro expanded mesenchymal stem cells (MSCs) to "one-step" procedures using bone marrow (BM) in toto (BM aspirate; BMA) or BM concentrate (BMC), have been developed. Recently, orthopedic researchers focused their attention on the clinical therapeutic potential of BMC and BMA for musculoskeletal regeneration. BMA is reported as an excellent source of cells and growth factors...
June 22, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28635177/engineering-human-bone-grafts-with-new-macroporous-calcium-phosphate-cement-scaffolds
#10
Martina Sladkova, Michael Palmer, Caroline Öhman, Jiayi Cheng, Shoug Al-Ansari, Munerah Saad, Håkan Engqvist, Giuseppe Maria de Peppo
Bone engineering opens the possibility to grow large amounts of tissue products by combining patient-specific cells with compliant biomaterials. Decellularized tissue matrices represent suitable biomaterials but availability, long processing time, excessive cost, and concerns on pathogen transmission have led to the development of biomimetic synthetic alternatives. We recently fabricated calcium phosphate cement (CPC) scaffolds with variable macroporosity using a facile synthesis method with minimal manufacturing steps, and demonstrated long-term biocompatibility in vitro...
June 21, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28611002/chondrogenesis-of-human-bone-marrow-mesenchymal-stem-cells-in-3-dimensional-photocrosslinked-hydrogel-constructs-effect-of-cell-seeding-density-and-material-stiffness
#11
Aaron X Sun, Hang Lin, Madalyn R Fritch, He Shen, Pete G Alexander, Michael DeHart, Rocky S Tuan
Three-dimensional hydrogel constructs incorporated with live stem cells that support chondrogenic differentiation and maintenance offer a promising regenerative route towards addressing the limited self-repair capabilities of articular cartilage. In particular, hydrogel scaffolds that augment chondrogenesis and recapitulate the native physical properties of cartilage, such as compressive strength, can potentially be applied in point-of-care procedures. We report here the synthesis of two new materials, [poly-l-lactic acid/polyethylene glycol/poly-l-lactic acid] (PLLA-PEG 1000) and [poly-d,l-lactic acid/polyethylene glycol/poly-d,l-lactic acid] (PDLLA-PEG 1000), that are biodegradable, biocompatible (>80% viability post fabrication), and possess high, physiologically relevant mechanical strength (∼1500 to 1800kPa)...
June 10, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28585995/outcomes-of-primary-total-knee-arthroplasty-in-patients-with-hematopoietic-stem-cell-transplantation
#12
Brian P Chalmers, Cameron K Ledford, Kevin I Perry, Tad M Mabry, Arlen D Hanssen, Matthew P Abdel
Patients who have undergone hematopoietic stem cell transplantation to treat underlying bone marrow pathology represent a unique and potentially high-risk patient population for total knee arthroplasty (TKA). This study retrospectively reviewed 15 TKA procedures performed on 11 patients with a history of hematopoietic stem cell transplantation. The authors analyzed patient survivorship; clinical outcomes, including complications; and implant survivorship. Mean follow-up was 5 years (range, 2-10 years). Patient survivorship free from mortality was 91% (95% confidence interval, 76%-100%) and 55% (95% confidence interval, 25%-85%) at 2 and 5 years, respectively...
June 6, 2017: Orthopedics
https://www.readbyqxmd.com/read/28577780/role-of-bone-marrow-aspirate-in-orthopedic-trauma
#13
REVIEW
Patrick C Schottel, Stephen J Warner
Bone marrow aspirate grafting entails mesenchymal stem cell-containing bone marrow harvesting and injection into a fracture site to promote bone formation. Although the use of bone marrow aspirate in orthopedic trauma is not widespread, an increasing number of studies are reporting clinical success. Advantages of using bone marrow aspirate are that it is readily obtainable, has low harvest morbidity, and can be easily and quickly injected. However, no universally accepted role for its use exists. Future studies directly comparing bone marrow aspirate with conventional techniques are needed to define its role in the treatment of orthopedic trauma patients...
July 2017: Orthopedic Clinics of North America
https://www.readbyqxmd.com/read/28562183/extracellular-matrices-for-bone-regeneration-a-literature-review
#14
Alaa Mansour, Mohamed Amine Mezour, Zahi Badran, Faleh Tamimi
The gold standard material for bone regeneration is still autologous bone, a mesenchymal tissue that consists mainly of extracellular matrix (ECM) (90% v/v) and little cellular content (10% v/v). However, the fact that decellularized allogenic bone grafts often present a clinical performance comparable to autologous bone grafts demonstrates the crucial role of ECM in bone regeneration. For long, the mechanism by which bone allografts function was not clear, but recent research has unveiled many unique characteristics of ECM that seem to play a key role in tissue regeneration...
July 12, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28555013/silver-nanoparticles-in-orthopedic-applications-new-insights-on-their-effects-on-osteogenic-cells
#15
Sara Castiglioni, Alessandra Cazzaniga, Laura Locatelli, Jeanette A M Maier
Infections of orthopedic implants are associated with high morbidity. The emergence of antibiotic resistant strains and the tendency of microbes to form biofilms on orthopedic devices prompt the individuation of novel antimicrobial agents. Silver nanoparticles represent an interesting alternative, but their effects on bone cells need to be clarified. We focused on osteoblast-like cells and on bone marrow-mesenchymal stem cells and found that these cells are rather resistant to the cytotoxic effects of silver nanoparticles, with a half maximal inhibitory concentration around 25 µg/mL as detected by MTT assay...
May 27, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28548484/controlled-release-of-naringin-in-metal-organic-framework-loaded-mineralized-collagen-coating-to-simultaneously-enhance-osseointegration-and-antibacterial-activity
#16
Mengfei Yu, Dongqi You, Junjun Zhuang, Suya Lin, Lingqing Dong, Shengtao Weng, Bin Zhang, Kui Cheng, Wenjian Weng, Huiming Wang
Two important goals in orthopedic implant research are to promote osseointegration and prevent infection. However, much previous effort has been focused on the design of coatings to either enhance osseointegration while ignoring antibacterial activity or vice versa, to prevent infection while ignoring bone integration. Here, we designed a multifunctional mineralized collagen coating on titanium with the aid of metal-organic framework (MOF) nanocrystals to control the release of naringin, a Chinese herbal medicine that could promote osseointegration and prevent bacterial infection...
June 5, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28548246/comparison-of-three-different-types-of-scaffolds-preseeded-with-human-bone-marrow-mononuclear-cells-on-the-bone-healing-in-a-femoral-critical-size-defect-model-of-the-athymic-rat
#17
Maren Janko, Julian Sahm, Alexander Schaible, Jan C Brune, Marlene Bellen, Katrin Schröder, Caroline Seebach, Ingo Marzi, Dirk Henrich
Large bone defects often pose major difficulties in orthopedic surgery. The application of long term cultured stem cells combined with a scaffold lead to a significant improvement of bone healing in recent experiments but is strongly restricted by EU-law. Bone marrow mononuclear cells (BMC), however, can be isolated and transplanted within few hours and have been proven effective in experimental models of bone healing. The effectivity of the BMC supported therapy might be influenced by the type of scaffold...
May 26, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28543623/regulatory-effect-of-microrna-34a-on-osteogenesis-and-angiogenesis-in-glucocorticoid-induced-osteonecrosis-of-the-femoral-head
#18
Xiaolong Zha, Bolin Sun, Rufan Zhang, Chen Li, Zuoqin Yan, Jifei Chen
BACKGROUND AND AIMS: Glucocorticoid-induced osteonecrosis of the femoral head (GIOFH) is a common and devastating orthopedic disease, and its underlying mechanism remains unclear. The aim of this study was to determine the role of microRNA-34a (mir-34a) in GIOFH. METHODS: C57 mouse mesenchymal stem cells (mMSCs) and human umbilical vein endothelial cells (HUVECs) were cultured with dexamethasone (Dex). A total of 48 adult rats were treated with glucocorticoids, and after the onset of GIOFH, each femoral head was removed...
May 20, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28515335/in-situ-bone-tissue-engineering-via-ultrasound-mediated-gene-delivery-to-endogenous-progenitor-cells-in-mini-pigs
#19
Maxim Bez, Dmitriy Sheyn, Wafa Tawackoli, Pablo Avalos, Galina Shapiro, Joseph C Giaconi, Xiaoyu Da, Shiran Ben David, Jayne Gavrity, Hani A Awad, Hyun W Bae, Eric J Ley, Thomas J Kremen, Zulma Gazit, Katherine W Ferrara, Gadi Pelled, Dan Gazit
More than 2 million bone-grafting procedures are performed each year using autografts or allografts. However, both options carry disadvantages, and there remains a clear medical need for the development of new therapies for massive bone loss and fracture nonunions. We hypothesized that localized ultrasound-mediated, microbubble-enhanced therapeutic gene delivery to endogenous stem cells would induce efficient bone regeneration and fracture repair. To test this hypothesis, we surgically created a critical-sized bone fracture in the tibiae of Yucatán mini-pigs, a clinically relevant large animal model...
May 17, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28510331/isolation-of-ready-to-use-adipose-derived-stem-cell-asc-pellet-for-clinical-applications-and-a-comparative-overview-of-alternate-methods-for-asc-isolation
#20
Edoardo Raposio, Nicolò Bertozzi
Current literature does not offer a standardized method to isolate adipose-derived stem cells (ASCs) for clinical applications and hence clinical studies using ASCs often show inconsistent results. Most of these studies borrow laboratory or benchside-derived protocols, which are complex, time consuming, and involve the use of chemical, animal-derived reagents. In this unit we describe a relatively simple and faster isolation protocol that allows collection of a ready-to-use ASC pellet for clinical application...
May 16, 2017: Current Protocols in Stem Cell Biology
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