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https://www.readbyqxmd.com/read/29451944/total-hip-arthroplasty-in-patients-with-avascular-necrosis-after-hematopoietic-stem-cell-transplantation
#1
Anita Vijapura, Harlan B Levine, Michele Donato, Mark A Hartzband, Melissa Baker, Gregg R Klein
The immunosuppressive regimens required for hematopoietic stem cell transplantation predispose recipients to complications, including avascular necrosis. Cancer-related comorbidities, immunosuppression, and poor bone quality theoretically increase the risk for perioperative medical complications, infection, and implant-related complications in total joint arthroplasty. This study reviewed 20 primary total hip arthroplasties for avascular necrosis in 14 patients. Outcomes were assessed at routine clinical visits and Harris hip scores were calculated...
February 16, 2018: Orthopedics
https://www.readbyqxmd.com/read/29445277/effects-of-silica-gentamicin-nanohybrids-on-osteogenic-differentiation-of-human-osteoblast-like-saos-2-cells
#2
Wei He, Dina A Mosselhy, Yudong Zheng, Qingling Feng, Xiaoning Li, Xing Yang, Lina Yue, Simo-Pekka Hannula
Introduction: In recent years, there has been an increasing interest in silica (SiO 2 ) nanoparticles (NPs) as drug delivery systems. This interest is mainly attributed to the ease of their surface functionalization for drug loading. In orthopedic applications, gentamicin-loaded SiO 2 NPs (nanohybrids) are frequently utilized for their prolonged antibacterial effects. Therefore, the possible adverse effects of SiO 2 -gentamicin nanohybrids on osteogenesis of bone-related cells should be thoroughly investigated to ensure safe applications...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29439516/expression-of-pluripotency-genes-in-chondrocyte-like-cells-differentiated-from-human-induced-pluripotent-stem-cells
#3
Ewelina Stelcer, Katarzyna Kulcenty, Marcin Rucinski, Karol Jopek, Tomasz Trzeciak, Magdalena Richter, Joanna P Wroblewska, Wiktoria M Suchorska
Human induced pluripotent stem cells (hiPSCs) constitute an important breakthrough in regenerative medicine, particularly in orthopedics, where more effective treatments are urgently needed. Despite the promise of hiPSCs only limited data on in vitro chondrogenic differentiation of hiPSCs are available. Therefore, we compared the gene expression profile of pluripotent genes in hiPSC-derived chondrocytes (ChiPS) to that of an hiPSC cell line created by our group (GPCCi001-A). The results are shown on heatmaps and plots and confirmed by Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) analysis...
February 12, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29435765/inflammation-mesenchymal-stem-cells-and-bone-regeneration
#4
REVIEW
Hongrui Liu, Dongfang Li, Yi Zhang, Minqi Li
Achieving satisfactory reconstruction of bone remains an important goal in orthopedic and dental conditions such as bone trauma, osteoporosis, arthritis, osteonecrosis, and periodontitis. Appropriate temporal and spatial differentiation of mesenchymal stem cells (MSCs) is essential for postnatal bone regeneration. Additionally, an acute inflammatory response is crucial at the onset of bone repair, while an adaptive immune response has important implications during late bone remodeling. Various reports have indicated bidirectional interactions between MSCs and inflammatory cells or molecules...
February 12, 2018: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29427540/novel-standardized-massive-bone-defect-model-in-rats-employing-an-internal-eight-hole-stainless-steel-plate-for-bone-tissue-engineering
#5
Huijie Jianga, Pengzhen Chenga, Donglin Lia, Junqin Lia, Jimeng Wanga, Yi Gaoa, Shuaishuai Zhanga, Tianqing Caoa, Chunmei Wanga, Liu Yanga, Guoxian Peia
Massive bone defects are a challenge in orthopedic research. Defective regeneration leads to bone atrophy, non-union of bone and physical morbidity. Large animals are important models, however, production costs are high, nursing is complex and evaluation methods are limited. A suitable laboratory animal model is required to explore the underlying molecular mechanism and cellular process of bone tissue engineering. We designed a stainless steel plate with eight holes; the middle two holes were used as a guide to create a standardized critical size defect in the femur of anesthetized rats...
February 10, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29415631/silk-biomaterials-mediated-mirna-functionalized-orthopedic-devices
#6
Eric James, Emily Van Doren, Chunmei Li, David L Kaplan
Silk-based bioresorbable medical devices, such as screws, plates and rods, have been under investigation due to their promising properties for orthopedic repairs. Options to functionalize these devices for enhanced control of bone regeneration would also exploit the compatible processing methods used to generate the devices. MicroRNAs are important regulators of bone maintenance and formation, and miRNA-based therapeutics have the potential to aid bone repair, utilizing a transient therapeutic approach with local bioactivity...
February 7, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29407153/bmp2-expressing-genetically-engineered-mesenchymal-stem-cells-on-composite-fibrous-scaffolds-for-enhanced-bone-regeneration-in-segmental-defects
#7
Shruthy Kuttappan, A Anitha, M G Minsha, Parvathy M Menon, T B Sivanarayanan, Lakshmi Sumitra Vijayachandran, Manitha B Nair
The treatment of critical sized bone defect remains a significant challenge in orthopedics. The objective of the study is to evaluate the effect of the combination of bone morphogenetic protein 2 (BMP2) expressing genetically engineered mesenchymal stem cells (MSCs) [MSCs engineered using a multimam vector, pAceMam1, an emerging gene delivery vector] and an osteoconductive scaffold [silica coated nanohydroxyapatite-gelatin reinforced with fibers] in enhancing bone regeneration in critical sized segmental defects...
April 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29405665/rapid-osteogenic-enhancement-of-stem-cells-in-human-bone-marrow-using-a-glycogen-synthease-kinase-3-beta-inhibitor-improves-osteogenic-efficacy-in-vitro-and-in-vivo
#8
Bret H Clough, Suzanne Zeitouni, Ulf Krause, Christopher D Chaput, Lauren M Cross, Akhilesh K Gaharwar, Carl A Gregory
Non-union defects of bone are a major problem in orthopedics, especially for patients with a low healing capacity. Fixation devices and osteoconductive materials are used to provide a stable environment for osteogenesis and an osteogenic component such as autologous human bone marrow (hBM) is then used, but robust bone formation is contingent on the healing capacity of the patients. A safe and rapid procedure for improvement of the osteoanabolic properties of hBM is, therefore, sought after in the field of orthopedics, especially if it can be performed within the temporal limitations of the surgical procedure, with minimal manipulation, and at point-of-care...
February 5, 2018: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29350343/syringic-acid-a-phenolic-acid-promotes-osteoblast-differentiation-by-stimulation-of-runx2-expression-and-targeting-of-smad7-by-mir-21-in-mouse-mesenchymal-stem-cells
#9
B Arumugam, K Balagangadharan, N Selvamurugan
Syringic acid (SA), a phenolic acid, has been used in Chinese and Indian medicine for treating diabetes but its role in osteogenesis has not yet been investigated. In the present study, at the molecular and cellular levels, we evaluated the effects of SA on osteoblast differentiation. At the cellular level, there was increased alkaline phosphatase (ALP) activity and calcium deposition by SA treatment in mouse mesenchymal stem cells (mMSCs). At the molecular level, SA treatment of these cells stimulated expression of Runx2, a bone transcription factor, and of osteoblast differentiation marker genes such as ALP, type I collagen, and osteocalcin...
January 19, 2018: Journal of Cell Communication and Signaling
https://www.readbyqxmd.com/read/29345324/autocrine-signals-increase-ovine-mesenchymal-stem-cells-migration-through-aquaporin-1-and-cxcr4-overexpression
#10
Alessandra Pelagalli, Anna Nardelli, Enrico Lucarelli, Antonella Zannetti, Arturo Brunetti
Sheep is a relevant large animal model that is frequently used to test innovative tissue engineering (TE) approaches especially for bone reconstruction. Mesenchymal stem cells (MSCs) are used in TE applications because they represent key component of adult tissue repair. Importantly, MSCs from different species show similar characteristics, which facilitated their application in translational studies using animal models. Nowadays, many researches are focusing on the use of ovine mesenchymal stem cells (oMSCs) in orthopedic preclinical settings for regenerative medicine purposes...
January 18, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29321058/graphene-oxide-conjugated-with-polymers-a-study-of-culture-condition-to-determine-whether-a-bacterial-growth-stimulant-or-an-antimicrobial-agent
#11
Ping-Ching Wu, Hua-Han Chen, Shih-Yao Chen, Wen-Lung Wang, Kun-Lin Yang, Chia-Hung Huang, Hui-Fang Kao, Jui-Cheng Chang, Chih-Li Lilian Hsu, Jiu-Yao Wang, Ting-Mao Chou, Wen-Shuo Kuo
BACKGROUND: The results showed that the deciding factor is the culture medium in which the bacteria and the graphene oxide (GO) are incubated at the initial manipulation step. These findings allow better use of GO and GO-based materials more and be able to clearly apply them in the field of biomedical nanotechnology. RESULTS: To study the use of GO sheets applied in the field of biomedical nanotechnology, this study determines whether GO-based materials [GO, GO-polyoxyalkyleneamine (POAA), and GO-chitosan] stimulate or inhibit bacterial growth in detail...
January 10, 2018: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/29312843/update-on-mesenchymal-stem-cell-therapies-for-cartilage-disorders
#12
EDITORIAL
Nikolaos K Paschos, Mackenzie L Sennett
Cartilage disorders, including focal cartilage lesions, are among the most common clinical problems in orthopedic practice. Left untreated, large focal lesions may result in progression to osteoarthritis, with tremendous impact on the quality of life of affected individuals. Current management strategies have shown only a modest degree of success, while several upcoming interventions signify better outcomes in the future. Among these, stem cell therapies have been suggested as a promising new era for cartilage disorders...
December 18, 2017: World Journal of Orthopedics
https://www.readbyqxmd.com/read/29305620/-correction-to-therapeutic-use-of-stem-cells-in-orthopedics
#13
C Chiari, S Walzer, D Stelzeneder, M Schreiner, R Windhager
No abstract text is available yet for this article.
January 5, 2018: Der Orthopäde
https://www.readbyqxmd.com/read/29287231/degradation-of-poly-%C3%AE%C2%B5-caprolactone-and-bio-interactions-with-mouse-bone-marrow-mesenchymal-stem-cells
#14
Sukanya V S, Mohanan P V
Bio-inspired scaffolds in bone tissue engineering using multipotential mesenchymal stem cells grow at a rapid rate found its successful use in orthopedic injury treatment. Poly(ε-caprolactone)/PCL is widely used in medical devices, tissue engineering, and drug delivery systems. Most desirable property of biodegradable polymer to be employed in medical application is synchronization of degradation with functional tissue regeneration. Limited studies have incorporated the degradation kinetics and implication of degradation products of pure unmodified PCL...
December 21, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29274103/osteogenic-differentiation-of-human-adipose-derived-stem-cells-on-chemically-crosslinked-carbon-nanomaterial-coatings
#15
Sunny C Patel, Owais Alam, Balaji Sitharaman
Carbon nanomaterial coatings have been widely investigated for many biomedical applications including bone tissue engineering. Current methods to fabricate carbon nanomaterial coatings are limited by specific substrate requirements and the lack of strong bonds between the nanomaterials. Furthermore, few studies compare the effect of carbon nanoparticle architecture on stem cell differentiation and mineralization for osteogenic differentiation. Herein, we report a study comparing chemically crosslinked carbon nanotubes (of various diameters), graphene nanoplatelets, and graphene nanoribbons coatings for adipose derived stem cell differentiation towards an osteogenic lineage...
December 23, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29228886/continuous-stimulation-with-differentiation-factors-is-necessary-to-enhance-osteogenic-differentiation-of-human-mesenchymal-stem-cells-in-vitro
#16
Bruno Reible, Gerhard Schmidmaier, Matthäus Prokscha, Arash Moghaddam, Fabian Westhauser
Bone defect treatment belongs to the most challenging fields in orthopedic surgery and requires the well-coordinated application of mesenchymal stem cells (MSC) and differentiation factors. MSC isolated from reaming material (RMSC) and iliac crest (BMSC) in combination with bone morphogenetic protein-7 (BMP-7) and insulin-like growth factor-1 (IGF-1) have been used. The short half-life of both factors limit their applications: a burst release of the factor can probably not induce sustainable differentiation...
October 2017: Growth Factors
https://www.readbyqxmd.com/read/29208555/hollow-mesoporous-zirconia-delivery-system-for-biomineralization-precursors
#17
Xue-Qing Huang, Hong-Ye Yang, Tao Luo, Cui Huang, Franklin R Tay, Li-Na Niu
Strategies based on the combination of nanocarrier delivery systems and scaffolds provide bone tissue engineering scaffolds with multifunctional capability. Zirconia, a biocompatible ceramic commonly used in orthopedic and dental implants, was used to synthesize hollow mesoporous nanocapsules for loading, storage and sustained release of a novel polyamine-stabilized liquid precursor phase of amorphous calcium phosphate (PAH-ACP) for collagen biomineralization and bone marrow stromal cells osteoinduction. Hollow mesoporous zirconia (hmZrO2) nanocapsules loaded with biomimetic precursors exhibited pH-sensitive release capability and good biocompatibility...
December 6, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29201060/osteogenic-differentiation-of-hdpscs-on-biogenic-bone-apatite-thin-films
#18
Michele Bianchi, Alessandra Pisciotta, Laura Bertoni, Matteo Berni, Alessandro Gambardella, Andrea Visani, Alessandro Russo, Anto de Pol, Gianluca Carnevale
A previous study reported the structural characterization of biogenic apatite (BAp) thin films realized by a pulsed electron deposition system by ablation of deproteinized bovine bone. Thin films annealed at 400°C exhibited composition and crystallinity degree very close to those of biogenic apatite; this affinity is crucial for obtaining faster osseointegration compared to conventional, thick hydroxyapatite (HA) coatings, for both orthopedics and dentistry. Here, we investigated the adhesion, proliferation, and osteogenic differentiation of human dental pulp stem cells (hDPCS) on as-deposited and heat-treated BAp and stoichiometric HA...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29156291/engineering-the-next-generation-tin-containing-%C3%AE-titanium-alloys-with-high-strength-and-low-modulus-for-orthopedic-applications
#19
Sumit Bahl, Suvam Das, Satyam Suwas, Kaushik Chatterjee
Metastable β Ti alloys are the new emerging class of biomaterial for load bearing orthopedic applications. However, these alloys in the single β phase microstructure have insufficient strength for use in load bearing applications. It is imperative to strengthen these alloys by carefully designed thermo-mechanical processing routes that typically involve aging treatment. In this investigation two newly designed Sn based β Ti alloys of composition Ti-32Nb-(2, 4) Sn are evaluated. The effects of Sn content on the mechanical properties and biological performance of these alloys processed through designed thermo-mechanical processing route are investigated...
November 11, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/29140074/facile-and-versatile-strategy-for-construction-of-anti-inflammatory-and-antibacterial-surfaces-with-polydopamine-mediated-liposomes-releasing-dexamethasone-and-minocycline-for-potential-implants-applications
#20
Xiao Xu, Lixin Wang, Zuyuan Luo, Yaofeng Ni, Haitao Sun, Xiang Gao, Yongliang Li, Siqi Zhang, Yan Li, Shicheng Wei
Reducing early nonbacterial inflammation induced by implanted materials and infection resulting from bacterial contamination around the implant-abutment interface could greatly accelerate implant osseointegration and reduce implant failure, which would be of clinical significance. In this study, we developed a facile and versatile strategy for the construction of anti-inflammatory and antibacterial surfaces on implants, such as orthopedic/dental implants. Briefly, the surfaces of polystyrene (PS) culture plates were first coated with polydopamine and then decorated with dexamethasone plus minocycline-loaded liposomes (Dex/Mino liposomes), which was confirmed by contact angle goniometry, X-ray photoelectron spectroscopy, and fluorescence microscopy...
November 15, 2017: ACS Applied Materials & Interfaces
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