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https://www.readbyqxmd.com/read/28515335/in-situ-bone-tissue-engineering-via-ultrasound-mediated-gene-delivery-to-endogenous-progenitor-cells-in-mini-pigs
#1
Maxim Bez, Dmitriy Sheyn, Wafa Tawackoli, Pablo Avalos, Galina Shapiro, Joseph C Giaconi, Xiaoyu Da, Shiran Ben David, Jayne Gavrity, Hani A Awad, Hyun W Bae, Eric J Ley, Thomas J Kremen, Zulma Gazit, Katherine W Ferrara, Gadi Pelled, Dan Gazit
More than 2 million bone-grafting procedures are performed each year using autografts or allografts. However, both options carry disadvantages, and there remains a clear medical need for the development of new therapies for massive bone loss and fracture nonunions. We hypothesized that localized ultrasound-mediated, microbubble-enhanced therapeutic gene delivery to endogenous stem cells would induce efficient bone regeneration and fracture repair. To test this hypothesis, we surgically created a critical-sized bone fracture in the tibiae of Yucatán mini-pigs, a clinically relevant large animal model...
May 17, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28510331/isolation-of-ready-to-use-adipose-derived-stem-cell-asc-pellet-for-clinical-applications-and-a-comparative-overview-of-alternate-methods-for-asc-isolation
#2
Edoardo Raposio, Nicolò Bertozzi
Current literature does not offer a standardized method to isolate adipose-derived stem cells (ASCs) for clinical applications and hence clinical studies using ASCs often show inconsistent results. Most of these studies borrow laboratory or benchside-derived protocols, which are complex, time consuming, and involve the use of chemical, animal-derived reagents. In this unit we describe a relatively simple and faster isolation protocol that allows collection of a ready-to-use ASC pellet for clinical application...
May 16, 2017: Current Protocols in Stem Cell Biology
https://www.readbyqxmd.com/read/28502902/retinoic-acid-mediated-anti-inflammatory-responses-in-equine-immune-cells-stimulated-by-lps-and-allogeneic-mesenchymal-stem-cells
#3
Leila Abdelhamid, Hayam Hussein, Mostafa Ghanem, Nour Eissa
Retinoic acid (RA), an active metabolite of vitamin A, has shown potential therapeutic immunomodulatory properties. Allogeneic mesenchymal stem cells (MSCs)-based therapy is an effective approach to induce tissue healing and regeneration in many equine orthopedic conditions. However, MSCs-based therapies induced inflammatory responses in vivo. This study aimed to: 1. Determine the effect of RA cell culture treatment on inflammatory responses of lipopolysaccharides (LPS)- and allogeneic MSCs-stimulated peripheral blood mononuclear cells (PBMCs)...
May 5, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28502669/biomimetic-tendon-extracellular-matrix-composite-gradient-scaffold-enhances-ligament-to-bone-junction-reconstruction
#4
Huanhuan Liu, Long Yang, Erchen Zhang, Rui Zhang, Dandan Cai, Shouan Zhu, Jisheng Ran, Varitsara Bunpetch, Youzhi Cai, Boon Chin Heng, Yejun Hu, Xuesong Dai, Xiao Chen, Hongwei Ouyang
Management of ligament/tendon-to-bone-junction healing remains a formidable challenge in the field of orthopedic medicine to date, due to deficient vascularity and multi-tissue transitional structure of the junction. Numerous strategies have been employed to improve ligament-bone junction healing, including delivery of stem cells, bioactive factors, and synthetic materials, but these methods are often inadequate at recapitulating the complex structure-function relationships at native tissue interfaces. Here, we developed an easily-fabricated and effective biomimetic composite to promote the regeneration of ligament-bone junction by physically modifying the tendon extracellular matrix (ECM) into a Random-Aligned-Random composite using ultrasound treatment...
May 11, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28482141/notch-pathway-is-active-during-osteogenic-differentiation-of-human-bone-marrow-mesenchymal-stem-cells-induced-by-pulsed-electromagnetic-fields
#5
Leila Bagheri, Agnese Pellati, Paola Rizzo, Giorgio Aquila, Leo Massari, Monica De Mattei, Alessia Ongaro
Pulsed electromagnetic fields (PEMFs) have been used to treat bone diseases, particularly non-union healing. Although it is known that PEMFs promote the osteogenic differentiation of human mesenchymal stem cells (hMSCs), to date PEMF molecular mechanisms remain not clearly elucidated. The Notch signaling is a highly conserved pathway which regulates cell fate decisions and skeletal development. The aim of this study was to investigate if the known PEMF-induced osteogenic effects may involve the modulation of the Notch pathway...
May 8, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28482125/electrical-stimulation-of-adipose-derived-mesenchymal-stem-cells-and-endothelial-cells-co-cultured-in-a-conductive-scaffold-for-potential-orthopedic-applications
#6
Jieyu Zhang, Koon Gee Neoh, En-Tang Kang
Electrical stimulation (ES) has emerged as a useful tool to regulate cell behavior, but the effect of ES on mesenchymal stem cell (MSC)/vasculogenic cell co-culture has not been investigated. Herein, human adipose-derived MSCs (AD-MSCs) and umbilical vein endothelial cells (HUVECs) were co-cultured in an electrically conductive polypyrrole/chitosan scaffold. Compared to AD-MSC monoculture, calcium deposition in the co-culture without and with ES (200 μA for 4 h per day) was 139% and 346% higher, respectively, after seven days...
May 8, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28474880/enhanced-bioactivity-and-bacteriostasis-of-surface-fluorinated-polyetheretherketone
#7
Meiling Chen, Liping Ouyang, Tao Lu, Heying Wang, Fanhao Meng, Yan Yang, Congqin Ning, Jingzhi Ma, Xuanyong Liu
Although polyetheretherketone (PEEK) has been considered as a potential orthopedic and dental application material due to its similar elastic modulus as bones, inferior osseointegration and bacteriostasis of PEEK hampers its clinical application. In this work, fluorinated PEEK was constructed via plasma immersion ion implantation (PIII) followed by hydrofluoric acid treatment to ameliorate the osseointegration and antibacterial properties of PEEK. The surface microstructure, composition, and hydrophilicity of all samples were investigated...
May 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28464787/directed-differentiation-and-paracrine-mechanisms-of-mesenchymal-stem-cells-potential-implications-for-tendon-repair-and-regeneration
#8
Bingyu Zhang, Qing Luo, Alexander Halim, Yang Ju, Yasuyuki Morita, Guanbin Song
Tendon is composed of connective tissue, is able to retract with high tensile force, and plays a significant role in musculoskeletal motion. However, inappropriate physical training or accidents often result in tendon injuries. So far, the functional healing of injured tendon is still a great challenge in orthopedics. Mesenchymal stem cells (MSCs) are multilineage cells with the ability to self-renew and differentiate into a variety of cell types, including tenocytes. The plasticity of MSCs gives rise to the chance of improved healing of injured tendons and even tissue-engineered tendons...
May 1, 2017: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28456657/topography-of-calcium-phosphate-ceramics-regulates-primary-cilia-length-and-tgf-receptor-recruitment-associated-with-osteogenesis
#9
Jingwei Zhang, Melis Dalbay, Xiaoman Luo, Erik Vrij, Davide Barbieri, Lorenzo Moroni, Joost D de Bruijn, Clemens A van Blitterswijk, Paul Chapple, Martin M Knight, Huipin Yuan
The surface topography of synthetic biomaterials is known to play a role in material-driven osteogenesis. Recent studies show that TGFβ signaling also initiates osteogenic differentiation. TGFβ signaling requires the recruitment of TGFβ receptors (TGFβR) to the primary cilia. In this study, we hypothesize that the surface topography of calcium phosphate ceramics regulates stem cell morphology, primary cilia structure and TGFβR recruitment to the cilium associated with osteogenic differentiation. We developed a 2D system using two types of tricalcium phosphate (TCP) ceramic discs with identical chemistry...
April 26, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28449254/bioresorbable-fe-mn-and-fe-mn-ha-materials-for-orthopedic-implantation-enhancing-degradation-through-porosity-control
#10
Michael Heiden, Eric Nauman, Lia Stanciu
Resorbable, porous iron-manganese-hydroxyapatite biocomposites with suitable degradation rates for orthopedic applications are prepared using salt-leaching for the first time. These transient biomaterials have the potential to replace inert, permanent implants that can suffer from long-term complications, or have to be surgically removed, leaving an unfavorable void. Fe30Mn-10HA materials are newly developed to address inadequate resorption rates of degradable materials proposed for orthopedic environments in the past...
April 27, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28434979/synergistic-effects-of-bmp-2-bmp-6-or-bmp-7-with-human-plasma-fibronectin-onto-hydroxyapatite-coatings-a-comparative-study
#11
Isabelle Brigaud, Rémy Agniel, Johanne Leroy Dudal, Sabrina Kellouche, Arnaud Ponche, Tahar Bouceba, Natalia Mihailescu, Mihai Sopronyi, Eric Viguier, Carmen Ristoscu, Felix Sima, Ion N Mihailescu, Ana Claudia O Carreira, Mari Cleide Sogayar, Olivier Gallet, Karine Anselme
Design of new osteoinductive biomaterials to reproduce an optimized physiological environment capable of recruiting stem cells and instructing their fate towards the osteoblastic lineage has become a priority in orthopedic surgery. This work aims at evaluating the bioactivity of BMP combined with human plasma fibronectin (FN/BMP) delivered in solution or coated onto titanium-hydroxyapatite (TiHA) surfaces. Herein, we focus on the comparison of in vitro osteogenic efficacy in mouse C2C12 pre-osteoblasts of three BMP members, namely: BMP-2, BMP-6 and BMP-7...
April 18, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28431400/local-delivery-of-hmgb1-in-gelatin-sponge-scaffolds-combined-with-mesenchymal-stem-cell-sheets-to-accelerate-fracture-healing
#12
Deting Xue, Wei Zhang, Erman Chen, Xiang Gao, Ling Liu, Chenyi Ye, Yanbin Tan, Zhijun Pan, Hang Li
Fracture nonunion and delayed union continue to pose challenges for orthopedic surgeons. In the present study, we combined HMGB1 gelatin sponges with MSC sheets to promote bone healing after surgical treatment of rat tibial fractures. The HMGB1 gelatin sponge scaffolds supported the expansion of mesenchymal stem cells (MSCs) and promoted the osteogenic differentiation of MSCs and MSC sheets. Lentiviral vectors were then used to overexpress HMGB1 in MSCs. The results indicated that HMGB1 promotes the osteogenic differentiation of MSCs through the STAT3 pathway...
April 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/28339434/platelet-rich-plasma-in-musculoskeletal-pathology-a-necessary-rescue-or-a-lost-cause
#13
Annu Navani, Gang Li, Joshua Chrystal
BACKGROUND: Platelet rich plasma (PRP) has been used for decades to facilitate surgical tissue repair; therefore, the current trend of percutaneously injecting PRP to theoretically enhance tissue regeneration and repair is a logical progression. Applications include treatment of osteoarthritis, tendinopathy, chondropathy, acute and chronic soft tissue injuries, muscle or ligament tear, as well as enhancement of healing after bone or tissue reconstruction. However, there is limited evidence to support the use of PRP in the abovementioned conditions...
March 2017: Pain Physician
https://www.readbyqxmd.com/read/28316107/applied-induced-pluripotent-stem-cells-in-combination-with-biomaterials-in-bone-tissue-engineering
#14
Abdolreza Ardeshirylajimi
Due to increasing of the orthopedic lesions and fractures in the world and limitation of current treatment methods, researchers, and surgeons paid attention to the new treatment ways especially to tissue engineering and regenerative medicine. Innovation in stem cells and biomaterials accelerate during the last decade as two main important parts of the tissue engineering. Recently, induced pluripotent stem cells (iPSCs) introduced as cells with highly proliferation and differentiation potentials that hold great promising features for used in tissue engineering and regenerative medicine...
March 18, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28303319/effect-of-sdf-1-cxcr4-signaling-antagonist-amd3100-on-bone-mineralization-in-distraction-osteogenesis
#15
Jia Xu, Yuanfeng Chen, Yang Liu, Jinfang Zhang, Qinglin Kang, Kiwai Ho, Yimin Chai, Gang Li
Distraction osteogenesis (DO) is a widely applied technique in orthopedics surgery, which involves rapid stem cell migration, homing, and differentiation. Interactions between the chemokine receptor Cxcr4 and its ligand, stromal derived factor-1 (SDF-1), regulate hematopoietic stem cell trafficking to the ischemic area and induce their subsequent differentiation. Here, we examined SDF-1 expression and further investigated the role of SDF-1/Cxcr4 signaling antagonist AMD3100 during bone regeneration in rat DO model...
March 16, 2017: Calcified Tissue International
https://www.readbyqxmd.com/read/28287922/co-seeding-human-endothelial-cells-with-human-induced-pluripotent-stem-cell-derived-mesenchymal-stem-cells-on-calcium-phosphate-scaffold-enhances-osteogenesis-and-vascularization-in-rats
#16
Xian Liu, Wenchuan Chen, Chi Zhang, Wahwah Thein-Han, Kevin Hu, Mark A Reynolds, Chongyun Bao, Ping Wang, Liang Zhao, Hockin H K Xu
A major challenge in repairing large bone defects with tissue-engineered constructs is the poor vascularization in the defect. The lack of vascular networks leads to insufficient oxygen and nutrients supply, which compromises the survival of seeded cells. To achieve favorable regenerative effects, prevascularization of tissue-engineered constructs by co-culturing of endothelial cells and bone cells is a promising strategy. The aim of this study was to investigate the effects of human-induced pluripotent stem cell-derived mesenchymal stem cells (hiPSC-MSCs) co-cultured with human umbilical vein endothelial cells (HUVECs) for prevascularization of calcium phosphate cement (CPC) scaffold on bone regeneration in vivo for the first time...
March 10, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28287912/osteogenic-differentiation-of-mesenchymal-stromal-cells-a-comparative-analysis-between-human-subcutaneous-adipose-tissue-and-dental-pulp
#17
Iolanda D'Alimonte, Filiberto Mastrangelo, Patricia Giuliani, Laura Pierdomenico, Marco Marchisio, Mariachiara Zuccarini, Patrizia Di Iorio, Raimondo Quaresima, Francesco Caciagli, Renata Ciccarelli
White adipose tissue is a source of mesenchymal stromal/stem cells (MSCs) that are actively studied for their possible therapeutic use in bone tissue repair/remodeling. To better appreciate the osteogenic potential of these cells, we compared some properties of MSCs from human subcutaneous adipose tissue [subcutaneous-adipose stromal cells (S-ASCs)] and dental pulp stem cell (DPSCs) of third-impacted molars, the latter representing a well-established MSC source. Both undifferentiated cell types showed similar fibroblast-like morphology and mesenchymal marker expression...
March 13, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28274194/the-combined-effects-of-nanotopography-and-sr-ion-for-enhanced-osteogenic-activity-of-bone-marrow-mesenchymal-stem-cells-bmscs
#18
Dandan Hu, Kai Li, Youtao Xie, Houhua Pan, Jun Zhao, Liping Huang, Xuebin Zheng
Both surface topography and chemistry have a significant influence on the biological performance of orthopedic implant coatings. In our study, a surface modification strategy embodying bioactive trace element incorporation and nanotopography construction was employed to enhance the osteogenic activity of calcium silicate (Ca-Si) coatings. We developed strontium-loaded nanolayer on plasma sprayed Ca-Si (CS) coating via hydrothermal treatment which was denoted as Sr-NT-CS. The original CS coating and the CS coating modified with similar nanotopography (NT-CS) were studied in parallel...
March 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/28259928/tissue-engineered-vascularized-periosteal-flap-enriched-with-msc-epcs-for-the-treatment-of-large-bone-defects-in-rats
#19
Christoph Nau, Dirk Henrich, Caroline Seebach, Katrin Schröder, John H Barker, Ingo Marzi, Johannes Frank
Vascularized periosteal flaps are used for complex cases if the reconstruction of large bone defects is necessary in modern trauma and orthopedic surgery. In this study, we combined this surgical procedure with β‑TCP scaffold and mesenchymal stem cells (MSCs) + endothelial progenitor cells (EPCs) as a tissue engineering approach to obtain optimum conditions for bone healing in rats. A critical size femoral defect was created in 80 rats allocated into 4 groups. Defects were treated according to the following protocol: i) vascularized periosteal flap alone; ⅱ) vascularized periosteal flap + β‑TCP scaffold; ⅲ) vascularized periosteal flap + β‑TCP scaffold + ligated vascular pedicle; and ⅳ) vascularized periosteal flap + β‑TCP scaffold + MSCs/EPCs...
April 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28247086/tsg-6-secreted-by-mesenchymal-stem-cells-suppresses-immune-reactions-influenced-by-bmp-2-through-p38-and-mek-mitogen-activated-protein-kinase-pathway
#20
Soyoun Um, Hui Young Kim, Joo-Hee Lee, In-Seok Song, Byoung Moo Seo
Bone morphogenetic protein 2 (BMP-2) has a critical function in bone and cartilage development and in repairing damaged organs and tissue. However, clinical use of BMP-2 at doses of 0.5-1 mg/ml for orthopedics has been associated with severe postoperative swelling requiring emergency surgical intervention. We determined whether a high concentration of BMP-2 induces inflammatory responses in macrophages and the suppression of osteogenesis in hMSCs. We obtained human periodontal ligament stem cells and bone marrow stem cells from the maxilla, i...
June 2017: Cell and Tissue Research
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