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Clostridium butyricum

Zeyu Lv, Yuting Wang, Ting Yang, Xue Zhan, Zhongyue Li, Huajian Hu, Tingyu Li, Jie Chen
To investigate whether gut microbiota is associated with vitamin A nutritional levels in children with persistent diarrhea, a total of 59 pediatric patients with persistent diarrhea aged 1-12 months were selected from the Department of Gastroenterology at the Children's Hospital of Chongqing Medical University, China. Subjects were hospitalized and divided into VA-deficient (n = 30) and VA-normal (n = 29) groups according to their venous serum retinol levels. Fecal samples from all 59 subjects were collected immediately after admission and analyzed by Illumina MiSeq for 16S rRNA genes to characterize the overall microbiota of the samples...
September 2016: Journal of Clinical Biochemistry and Nutrition
Shelby R Hayes, Ashley J Vargas
: Goldenberg JZ, Lytvyn L, Steurich J, Parkin P, Mahant S, Johnston BC. Probiotics for the prevention of pediatric antibiotic-associated diarrhea.Cochrane Database Syst Rev2015, Issue 12. Art. No.: CD004827. BACKGROUND: Antibiotics are frequently prescribed in children. They alter the microbial balance within the gastrointestinal tract, commonly resulting in antibiotic-associated diarrhea (AAD). Probiotics may prevent AAD via restoration of the gut microflora...
August 26, 2016: Explore: the Journal of Science and Healing
Yuan-Fan Deng, Yuan-Yuan Liu, Yi-Tao Zhang, Yan Wang, Juan-Boo Liang, Vincenzo Tufarelli, Vito Laudadio, Xin-Di Liao
BACKGROUND: The efficacy and role of inulin in the mitigation of enteric sulfur-containing odor gases hydrogen sulfide (H2 S) and methyl mercaptan (CH3 SH) in pigs were examined in this study. Twelve Duroc × Landrace × Yorkshire male finisher pigs (60.7 ± 1.9 kg), housed individually in open-circuit respiration chambers, were randomly assigned to two dietary groups, namely basal diet (control) and basal diet supplemented with 1% (w/w) inulin. At the end of the 45 day experiment, pigs were slaughtered and volatile fatty acid (VFA) concentration, sulfate radical (SO4(2)(-) ) concentration, population of sulfate-reducing bacteria (SRB) and expression of methionine gamma-lyase (MGL) gene were determined in contents from the caecum, colon (two segments) and rectum...
September 24, 2016: Journal of the Science of Food and Agriculture
Shi-Fa Zhang, Zong-Sheng Tang, Ling Tong, Xiang-Xiang Tao, Qi-Feng Suo, Xue-Mei Xu
BACKGROUND & AIMS: Probiotics is recognized to promote growth performance and immune function via balancing the intestinal microflora. Live clostridium butyricum and bifidobacterium combined powder (LCBBCP) has been widely to treat intestinal dysbacteriosis in newborns in China. This study was undertaken to investigate the effects of the combined probiotics on the expression of B and T lymphocyte attenuator (BTLA) on CD4(+) T cells and the differentiation of lymphocyte subsets in late preterm infants...
September 10, 2016: Microbial Pathogenesis
Quanxin Gao, Yingping Xiao, Chenjie Zhang, Minghua Min, Shiming Peng, Zhaohong Shi
Toll-like receptor 2 (TLR2) has been shown to play a crucial role in the host defense of pathogenic microbes in innate immunity. In this study, the full-length cDNA of TLR2 in silvery pomfret (Pampus argenteus) was cloned by homology cloning and the rapid amplification of cDNA ends (RACE) technique. The complete cDNA sequence of TLR2 was 2932 bp, containing an open reading frame (ORF) of 2469 bp encoding 822 amino acids. A multiple alignment analysis of the silvery pomfret TLR2 protein-coding sequence with other known TLR2 sequences from Oplegnathus fasciatus, Epinephelus coioides, Larimichthys crocea, Miichthys miiuy, Oreochromis niloticus, Paralichthys olivaceus, Trematomus bernacchii, Sparus aurata, and Chionodraco hamatus exhibited a high degree of homology of 78...
August 26, 2016: Fish & Shellfish Immunology
Min Cai, Lu Zeng, Lin-Jing Li, Li-Hua Mo, Rui-Di Xie, Bai-Sui Feng, Peng-Yuan Zheng, Zhi-Gang Liu, Zhan-Ju Liu, Ping-Chang Yang
BACKGROUND: Hypersensitivity reaction to certain allergens plays a role in the pathogenesis of inflammatory bowel disease (IBD). This study aims to observe the effect of specific immunotherapy in a group of IBD patients. METHODS: Patients with both ulcerative colitis (UC) and food allergy were recruited into this study. Food allergy was diagnosed by skin prick test and serum specific IgE. The patients were treated with specific immunotherapy (SIT) and Clostridium butyricum (CB) capsules...
2016: Allergy, Asthma, and Clinical Immunology
B J Tindall
In a recent publication that attempts to deal with the growing problem of taxa being added to the genus Clostridium that are outside of Clostridium (16S rRNA) group I a solution is proposed that seeks to limit the genus Clostridium Prazmowski 1880 (Approved Lists 1980) to a small number of species "related" to the type species, Clostridium butyricum Prazmowski 1880 (Approved Lists 1980) it has been proposed that this genus should also include members of the genus Sarcina Goodsir 1842 (Approved Lists 1980), Sarcina maxima Lindner 1888 (Approved Lists 1980) and Sarcina ventriculi Goodsir 1842 (Approved Lists 1980) the latter being the nomenclatural type of the genus Sarcina Goodsir 1842 (Approved Lists 1980)...
August 2, 2016: International Journal of Systematic and Evolutionary Microbiology
Ling-Zhi Xu, Li-Tao Yang, Shu-Qi Qiu, Gui Yang, Xiang-Qian Luo, Bei-Ping Miao, Xiao-Rui Geng, Zhi-Qiang Liu, Jun Liu, Zhong Wen, Shuai Wang, Huan-Ping Zhang, Jing Li, Zhi-Gang Liu, Hua-Bin Li, Ping-Chang Yang
The therapeutic efficacy of allergen specific immunotherapy (SIT) on allergic diseases is to be improved. Probiotics can regulate immune response. This study aims to promote the effect of SIT on allergic rhinitis (AR) by co-administration with Clostridium butyricum (Cb). In this study, patients with AR sensitized to mite allergens were enrolled to this study, and treated with SIT or/and Cb. The therapeutic efficacy was evaluated by the total nasal symptom scores (NSS), medication scores, serum specific IgE levels and T helper (Th)2 cytokine levels...
July 29, 2016: Oncotarget
Richard Garceau, Christine Bourque, Louise Thibault, Jean-Charles Côté, Jean Longtin, Marc-Christian Domingo
An 88-year-old man was admitted to the hospital with worsening malaise, fever, and weakness. Anaerobic blood culture bottles revealed the presence of an anaerobic, Gram-positive sporulated bacillus. Empirical antibiotherapy with intravenous piperacillin-tazobactam was initiated. The patient defervesced after four days and was switched to oral amoxicillin on his 6th day of antibiotic therapy and later discharged from the hospital. Four months later, he had recovered. The bacterium was initially identified as Clostridium butyricum using anaerobic manual identification panel...
2016: Canadian Journal of Infectious Diseases & Medical Microbiology
Guangyu Li, Xiang Zeng, Xiupian Liu, Xiaobo Zhang, Zongze Shao
An anaerobic, Gram-stain-positive, spore-forming bacterium, designated DY30321T was isolated from a hydrothermal sulfides mixture sample collected during the cruise DY30 of R/V Da Yang Yi Hao. Cells of strain DY30321T were rod-shaped with rounded ends, non-flagellum and were not motile. Strain DY30321T grew optimally at pH 8.0, 30 °C, with salinity (sea salts) of 30-40 g.L-1. The principal fatty acids of strain DY30321T were C14:0 and summed Feature 1 (comprising iso H-C15:1/3OH-C13:0, 13.9 %). The predominant polar lipids of strain DY30321T were diphosphatidylglycerol, phosphatidylcholine and phosphatidylethanolamine...
July 28, 2016: International Journal of Systematic and Evolutionary Microbiology
Michelangelo Storari, Sandra Kulli, Daniel Wüthrich, Rémy Bruggmann, Hélène Berthoud, Emmanuelle Arias-Roth
Clostridium tyrobutyricum is the main microorganism responsible for the late blowing defect in hard and semi-hard cheeses, causing considerable economic losses to the cheese industry. Deeper knowledge of the metabolic requirements of this microorganism can lead to the development of more effective control approaches. In this work, the amino acids and B vitamins essential for sustaining the growth of C. tyrobutyricum were investigated using a genomic approach. As the first step, the genomes of four C. tyrobutyricum strains were analyzed for the presence of genes putatively involved in the biosynthesis of amino acids and B vitamins...
October 2016: Food Microbiology
Paul A Lawson, Diane M Citron, Kerin L Tyrrell, Sydney M Finegold
The recent proposal by Lawson and Rainey (2015) to restrict the genus Clostridium to Clostridium butyricum and related species has ramifications for the members of the genera that fall outside this clade that should not be considered as Clostridium sensu stricto. One such organism of profound medical importance is Clostridioides difficile that is a major cause of hospital-acquired diarrhea and mortality in individuals. Based on 16S rRNA gene sequence analysis, the closest relative of Clostridium difficile is Clostridium mangenotii with a 94...
August 2016: Anaerobe
Joseph W LaMattina, Nicholas D Keul, Pierre Reitzer, Suraj Kapoor, Felipe Galzerani, Daniel J Koch, Iuri E Gouvea, William N Lanzilotta
Glycyl radical enzymes (GREs) represent a diverse superfamily of enzymes that utilize a radical mechanism to catalyze difficult, but often essential, chemical reactions. In this work we present the first biochemical and structural data for a GRE-type diol dehydratase from the organism Roseburia inulinivorans (RiDD). Despite high sequence (48% identity) and structural similarity to the GRE-type glycerol dehydratase from Clostridium butyricum, we demonstrate that the RiDD is in fact a diol dehydratase. In addition, the RiDD will utilize both (S)-1,2-propanediol and (R)-1,2-propanediol as a substrate, with an observed preference for the S enantiomer...
July 22, 2016: Journal of Biological Chemistry
Dan Jiang, Zhen Fang, Siew-Xian Chin, Xiao-Fei Tian, Tong-Chao Su
Biohydrogen production has received widespread attention from researchers in industry and academic fields. Response surface methodology (RSM) was applied to evaluate the effects of several key variables in anaerobic fermentation of glucose with Clostridium butyrium, and achieved the highest production rate and yield of hydrogen. Highest H2 yield of 2.02 mol H2/mol-glucose was achieved from 24 h bottle fermentation of glucose at 35 °C, while the composition of medium was (g/L): 15.66 glucose, 6.04 yeast extract, 4 tryptone, 3 K2HPO4, 3 KH2PO4, 0...
2016: Scientific Reports
Waraporn Apiwatanapiwat, Pilanee Vaithanomsat, Chakrit Tachaapaikoon, Khanok Ratanakhanokchai, Akihiko Kosugi
To improve its 1,3-propanediol (1,3-PD) production, Clostridium butyricum was cultivated on glycerol medium supplemented with cassava pulp (CP). At small concentrations, the CP improved the 1,3-PD productivity of C. butyricum from (0.25±0.01)g/L/h (glycerol alone) to (0.43±0.02)g/L/h (glycerol+2g/L CP) after 24h fermentation.
July 20, 2016: Journal of Biotechnology
Bin Lan, Fan Yang, Dong Lu, Zhenlv Lin
The aberrant T cell activation plays an important role in the pathogenesis of intestinal inflammation, such as ulcerative colitis (UC). C. butyricum (Cb) is a probiotic and has been employed in the treatment of immune diseases. This study tests a hypothesis that specific immunotherapy (SIT) plus oral Cb (an over-the-counter probiotic) alleviates the UC symptoms. In this study, we conducted a randomized, double-blind, clinical study at our hospital. A total of 80 patients with relapsing-remitting ulcerative colitis and high levels of specific IgE antibody was randomly divided into 4 groups, and were treated with SIT or/and Cb, or placebo, respectively for 1 year...
2016: Scientific Reports
Haixiao Shang, Jia Sun, Yong Q Chen
Obesity is associated with a cluster of metabolic disorders and systemic low-grade inflammation involving multiple organs. Recent findings have suggested that intestine is a key organ altered in response to high fat diet (HFD) feeding. Probiotics mainly lactobacillus strains have earlier been implicated in alleviating metabolic disorders. Here we aimed to examine the effects of a naturally occurring butyrate-producing probiotic clostridium butyricum CGMCC0313.1 (CB0313.1) in limiting the development of HFD-induced obesity...
2016: PloS One
Chunjie Zhu, Baishan Fang, Shizhen Wang
The effects of culture conditions on the kinetic behavior of 1,3-propanediol (PD) fermentation were investigated with a kinetic model. First, with initial glycerol concentration (S0) increasing, μmax and PD inhibition increased. Glycerol assimilation was harder and a little glycerol was consumed on cell maintenance at high S0. Second, with yeast extract concentration increasing, PD inhibition decreased. However, μmax decreased and glycerol assimilation became harder. It seems that the stimulus effect of yeast extract resulted from decreased PD inhibition...
July 2016: Bioresource Technology
Jing Sun, Fangyan Wang, Zongxin Ling, Xichong Yu, Wenqian Chen, Haixiao Li, Jiangtao Jin, Mengqi Pang, Huiqing Zhang, Junjie Yu, Jiaming Liu
Diabetes is known to exacerbate cerebral ischemia/reperfusion (I/R) injury. Here, we investigated the effects of Clostridium butyricum on cerebral I/R injury in the diabetic mice subjected to 30min of bilateral common carotid arteries occlusion (BCCAO). The cognitive impairment, the blood glucose level, neuronal injury, apoptosis, and expressions of Akt, phospho-Akt (p-Akt), and caspase-3 level were assessed. Meanwhile, the changes of gut microbiota in composition and diversity in the colonic feces were evaluated...
March 29, 2016: Brain Research
Jian-Zhong Liu, Wu Xu, Andrei Chistoserdov, Rakesh K Bajpai
To date, two types of glycerol dehydratases have been reported: coenzyme B12-dependent and coenzyme B12-independent glycerol dehydratases. The three-dimensional structure of the former is a dimer of αβγ heterotrimer, while that of the latter is a homodimer. Their mechanisms of reaction are typically enzymatic radical catalysis. Functional radical in both the glycerol dehydratases is the adenosyl radical. However, the adenosyl radical in the former originates from coenzyme B12 by homolytic cleavage, and that in the latter from S-adenosyl-methionine...
July 2016: Applied Biochemistry and Biotechnology
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