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In vivo patch clamp recording

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https://www.readbyqxmd.com/read/29758584/inhibition-of-sympathetic-sprouting-in-ccd-rats-by-lacosamide
#1
Yuying Wang, Fuquan Huo
BACKGROUND: Early hyperexcitability activity of injured nerve/neuron is critical for developing sympathetic nerve sprouting within dorsal root ganglia (DRG). Since lacosamide (LCM), an anticonvulsant, inhibits Na+ channel. The present study tried to test the potential effect of LCM on inhibiting sympathetic sprouting in vivo. METHODS: LCM (50 mg/kg) was daily injected intraperitoneally into rats subjected to chronic compression DRG (CCD), an animal model of neuropathic pain that exhibits sympathetic nerve sprouting, for the 1st 7 days after injury...
May 14, 2018: European Journal of Pain: EJP
https://www.readbyqxmd.com/read/29740286/optogenetic-control-of-neural-circuits-in-the-mongolian-gerbil
#2
Stefan Keplinger, Barbara Beiderbeck, Stylianos Michalakis, Martin Biel, Benedikt Grothe, Lars Kunz
The Mongolian gerbil ( Meriones unguiculatus ) is widely used as a model organism for the human auditory system. Its hearing range is very similar to ours and it uses the same mechanisms for sound localization. The auditory circuits underlying these functions have been characterized. However, important mechanistic details are still under debate. To elucidate these issues, precise and reversible optogenetic manipulation of neuronal activity in this complex circuitry is required. However, genetic and genomic resources for the Mongolian gerbil are poorly developed...
2018: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/29650545/regulation-of-blood-pressure-by-targeting-ca-v-1-2-galectin-1-protein-interaction
#3
Zhenyu Hu, Guang Li, Jiong-Wei Wang, Suet Yen Chong, Dejie Yu, Xiaoyuan Wang, Jia Lin Soon, Mui Cheng Liang, Yuk Peng Wong, Na Huang, Henry M Colecraft, Ping Liao, Tuck Wah Soong
Background -L-type CaV 1.2 channels play crucial roles in regulation of blood pressure. Galectin-1 (Gal-1), has been reported to bind to the I-II loop of CaV 1.2 channels to reduce their current density. However, the mechanistic understanding for the down-regulation of CaV 1.2 channels by Gal-1, and whether Gal-1 plays a direct role in blood pressure regulation remain unclear. Methods - In vitro experiments involving co-IP, western blot, patch-clamp recordings, immunohistochemistry and pressure myography were used to evaluate the molecular mechanisms by which Gal-1 down-regulates CaV 1...
April 12, 2018: Circulation
https://www.readbyqxmd.com/read/29608176/preparations-and-protocols-for-whole-cell-patch-clamp-recording-of-xenopus-laevis-tectal-neurons
#4
Zhenyu Liu, Katelynne B Donnelly, Kara G Pratt
The Xenopus tadpole retinotectal circuit, comprised of the retinal ganglion cells (RGCs) in the eye which form synapses directly onto neurons in the optic tectum, is a popular model to study how neural circuits self-assemble. The ability to carry out whole cell patch clamp recordings from tectal neurons and to record RGC-evoked responses, either in vivo or using a whole brain preparation, has generated a large body of high-resolution data about the mechanisms underlying normal, and abnormal, circuit formation and function...
March 15, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29579315/co-expression-of-%C3%AE-and-%C3%A2-opioid-receptors-by-mouse-colonic-nociceptors
#5
Raquel Guerrero-Alba, Eduardo Emmanuel Valdez-Morales, Nestor Nivardo Jiménez-Vargas, Romke Bron, Daniel Poole, David Reed, Joel Castro, Melissa Campaniello, Patrick A Hughes, Stuart M Brierley, Nigel Bunnett, Alan E Lomax, Stephen Vanner
BACKGROUND AND PURPOSE: To better understand opioid signaling in visceral nociceptors, we examined the expression and selective activation of mu (MOR) and delta opioid receptors (DOR) by dorsal root ganglia (DRG) neurons innervating the mouse colon. EXPERIMENTAL APPROACH: DRG neurons projecting to the colon were identified by retrograde tracing. DOR-GFP reporter mice, in situ hybridization, single-cell RT-PCR, and MOR-specific antibodies were used to characterize expression of MOR and DOR...
March 26, 2018: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29560580/presynaptic-regulation-of-tonic-inhibition-by-neuromodulatory-transmitters-in-the-basal-amygdala
#6
S Meis, T Endres, T Munsch, V Lessmann
Tonic inhibition mediated by ambient levels of GABA that activate extrasynaptic GABAA receptors emerges as an essential factor that tunes neuronal network excitability in vitro and shapes behavioral responses in vivo. To address the role of neuromodulatory transmitter systems on this type of inhibition, we employed patch clamp recordings in mouse amygdala slice preparations. Our results show that the current amplitude of tonic inhibition (Itonic ) in projection neurons of the basal amygdala (BA) is increased by preincubation with the neurosteroid THDOC, while the benzodiazepine diazepam is ineffective...
March 20, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29507411/nontoxic-double-deletion-mutant-rabies-viral-vectors-for-retrograde-targeting-of-projection-neurons
#7
Soumya Chatterjee, Heather A Sullivan, Bryan J MacLennan, Ran Xu, YuanYuan Hou, Thomas K Lavin, Nicholas E Lea, Jacob E Michalski, Kelsey R Babcock, Stephan Dietrich, Gillian A Matthews, Anna Beyeler, Gwendolyn G Calhoon, Gordon Glober, Jennifer D Whitesell, Shenqin Yao, Ali Cetin, Julie A Harris, Hongkui Zeng, Kay M Tye, R Clay Reid, Ian R Wickersham
Recombinant rabies viral vectors have proven useful for applications including retrograde targeting of projection neurons and monosynaptic tracing, but their cytotoxicity has limited their use to short-term experiments. Here we introduce a new class of double-deletion-mutant rabies viral vectors that left transduced cells alive and healthy indefinitely. Deletion of the viral polymerase gene abolished cytotoxicity and reduced transgene expression to trace levels but left vectors still able to retrogradely infect projection neurons and express recombinases, allowing downstream expression of other transgene products such as fluorophores and calcium indicators...
April 2018: Nature Neuroscience
https://www.readbyqxmd.com/read/29474819/direct-evidence-for-high-affinity-blockade-of-na-v-1-6-channel-subtype-by-huwentoxin-iv-spider-peptide-using-multiscale-functional-approaches
#8
Tânia C Gonçalves, Rachid Boukaiba, Jordi Molgó, Muriel Amar, Michel Partiseti, Denis Servent, Evelyne Benoit
The Chinese bird spider huwentoxin-IV (HwTx-IV) is well-known to be a highly potent blocker of NaV 1.7 subtype of voltage-gated sodium (NaV ) channels, a genetically validated analgesic target, and thus promising as a potential lead molecule for the development of novel pain therapeutics. In the present study, the interaction between HwTx-IV and NaV 1.6 channel subtype was investigated using multiscale (from in vivo to individual cell) functional approaches. HwTx-IV was approximatively 2 times more efficient than tetrodotoxin (TTX) to inhibit the compound muscle action potential recorded from the mouse skeletal neuromuscular system in vivo, and 30 times more effective to inhibit nerve-evoked than directly-elicited muscle contractile force of isolated mouse hemidiaphragms...
May 1, 2018: Neuropharmacology
https://www.readbyqxmd.com/read/29471715/the-effect-of-acute-dissociation-on-the-electrophysiological-properties-of-rat-dorsal-root-ganglion-neurons
#9
Yuanlong Song, Linlin Gao
The acutely dissociated neurons from the dorsal root ganglia (DRGs) are extensively used. The effects of acute dissociation on the properties of these neurons are, however, not clear. In this study, the action potentials (APs) were recorded from both acutely dissociated and in vivo identified DRG neurons with patch clamp and sharp electrode recording techniques, respectively. We found that acute dissociation slowed both the depolarizing and repolarizing rate of APs, and elongated the AP duration (APD). The lower recording temperature presented in the acutely dissociated neurons contributed to about 10% of these differences...
March 2018: Somatosensory & Motor Research
https://www.readbyqxmd.com/read/29463643/hyperactivity-of-anterior-cingulate-cortex-areas-24a-24b-drives-chronic-pain-induced-anxiodepressive-like-consequences
#10
Jim Sellmeijer, Victor Mathis, Sylvain Hugel, Xu-Hui Li, Qian Song, Qi-Yu Chen, Florent Barthas, Pierre-Eric Lutz, Meltem Karatas, Andreas Luthi, Pierre Veinante, Ad Aertsen, Michel Barrot, Min Zhuo, Ipek Yalcin
Pain associates both sensory and emotional aversive components, and often leads to anxiety and depression when it becomes chronic. Here, we characterized, in a mouse model, the long-term development of these sensory and aversive components as well as anxiodepressive-like consequences of neuropathic pain and determined their electrophysiological impact on the anterior cingulate cortex (ACC, cortical areas 24a/24b). We show that these symptoms of neuropathic pain evolve and recover in different time courses following nerve injury in male mice...
March 21, 2018: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29452828/glutamatergic-ventral-pallidal-neurons-modulate-activity-of-the-habenula-tegmental-circuitry-and-constrain-reward-seeking
#11
Jessica Tooley, Lauren Marconi, Jason Bondoc Alipio, Bridget Matikainen-Ankney, Polymnia Georgiou, Alexxai V Kravitz, Meaghan C Creed
BACKGROUND: The ability to appropriately integrate and respond to rewarding and aversive stimuli is essential for survival. The ventral pallidum (VP) plays a critical role in processing both rewarding and aversive stimuli. However, the VP is a heterogeneous structure, and how VP subpopulations integrate into larger reward networks to ultimately modulate these behaviors is not known. We identify a noncanonical population of glutamatergic VP neurons that play a unique role in responding to aversive stimuli and constraining inappropriate reward seeking...
January 12, 2018: Biological Psychiatry
https://www.readbyqxmd.com/read/29430734/gaba-and-serotonin-expressing-neurons-take-part-in-inhibitory-as-well-as-excitatory-input-pathways-to-the-circadian-clock-of-the-madeira-cockroach-rhyparobia-maderae
#12
Maria Giese, Julia Gestrich, Azar Massah, Jonas Peterle, HongYing Wei, Monika Stengl
In the Madeira cockroach, pigment-dispersing factor-immunoreactive (PDF-ir) neurons innervating the circadian clock, the accessory medulla (AME) in the brain's optic lobes, control circadian behaviour. Circadian activity rhythms are entrained to daily light-dark cycles only by compound eye photoreceptors terminating in the lamina and medulla. Still, it is unknown which neurons connect the photoreceptors to the clock to allow for light entrainment. Here, we characterized by multiple-label immunocytochemistry the serotonin (5-HT)-ir anterior fibre fan and GABA-ir pathways connecting the AME- and optic lobe neuropils...
February 11, 2018: European Journal of Neuroscience
https://www.readbyqxmd.com/read/29415216/distinct-neocortical-progenitor-lineages-fine-tune-neuronal-diversity-in-a-layer-specific-manner
#13
Teresa Guillamon-Vivancos, William A Tyler, Maria Medalla, Wayne Wei-En Chang, Mayumi Okamoto, Tarik F Haydar, Jennifer I Luebke
How the variety of neurons that organize into neocortical layers and functional areas arises is a central question in the study of cortical development. While both intrinsic and extrinsic cues are known to influence this process, whether distinct neuronal progenitor groups contribute to neuron diversity and allocation is poorly understood. Using in vivo genetic fate-mapping combined with whole-cell patch clamp recording, we show that the firing pattern and apical dendritic morphology of excitatory neurons in layer 4 of the barrel cortex are specified in part by their neural precursor lineage...
February 3, 2018: Cerebral Cortex
https://www.readbyqxmd.com/read/29412518/atrium-specific-ion-channels-in-the-zebrafish-a-role-of-i-kach-in-atrial-repolarization
#14
M A Skarsfeldt, S H Bomholtz, P R Lundegaard, A Lopez-Izquierdo, M Tristani-Firouzi, B H Bentzen
AIM: The zebrafish has emerged as a novel model for investigating cardiac physiology and pathology. The aim of this study was to investigate the atrium-specific ion channels responsible for shaping the atrial cardiac action potential in zebrafish. METHODS: Using quantitative polymerase chain reaction, we assessed the expression level of atrium-specific potassium channels. The functional role of these channels was studied by patch clamp experiments on isolated atrial and ventricular cardiomyocytes and by optical mapping of explanted adult zebrafish hearts...
February 7, 2018: Acta Physiologica
https://www.readbyqxmd.com/read/29408225/partial-trkb-receptor-activation-suppresses-cortical-epileptogenesis-through-actions-on-parvalbumin-interneurons
#15
Feng Gu, Isabel Parada, Tao Yang, Frank M Longo, David A Prince
Post-traumatic epilepsy is one of the most common and difficult to treat forms of acquired epilepsy worldwide. Currently, there is no effective way to prevent post-traumatic epileptogenesis. It is known that abnormalities of interneurons, particularly parvalbumin-containing interneurons, play a critical role in epileptogenesis following traumatic brain injury. Thus, enhancing the function of existing parvalbumin interneurons might provide a logical therapeutic approach to prevention of post-traumatic epilepsy...
May 2018: Neurobiology of Disease
https://www.readbyqxmd.com/read/29405580/in-vitro-and-in-vivo-characterization-of-modulation-of-the-vacuolar-cation-channel-trpy1-from-saccharomyces-cerevisiae
#16
Shin Hamamoto, Yasuo Mori, Isamu Yabe, Nobuyuki Uozumi
Saccharomyces cerevisiae possesses a transient receptor potential (TRP) channel homolog TRPY1 in its vacuolar membrane, considered to be an ancestral TRP channel. So far, studies have focused on the channel properties of TRPY1, but its regulation and physiologic role remained to be elucidated. Here, we investigated TRPY1 channel function in vitro and in vivo. Patch-clamp recording of TRPY1 in yeast vacuolar membranes showed that Ca2+ on the lumen side inhibited TRPY1-mediated channel activity, whereas luminal Zn2+ increased the currents...
March 2018: FEBS Journal
https://www.readbyqxmd.com/read/29335317/reduced-n-type-ca-2-channels-in-atrioventricular-ganglion-neurons-are-involved-in-ventricular-arrhythmogenesis
#17
Dongze Zhang, Huiyin Tu, Liang Cao, Hong Zheng, Robert L Muelleman, Michael C Wadman, Yu-Long Li
BACKGROUND: Attenuated cardiac vagal activity is associated with ventricular arrhythmogenesis and related mortality in patients with chronic heart failure. Our recent study has shown that expression of N-type Ca2+ channel α-subunits (Cav 2.2-α) and N-type Ca2+ currents are reduced in intracardiac ganglion neurons from rats with chronic heart failure. Rat intracardiac ganglia are divided into the atrioventricular ganglion (AVG) and sinoatrial ganglion. Ventricular myocardium receives projection of neuronal terminals only from the AVG...
January 15, 2018: Journal of the American Heart Association
https://www.readbyqxmd.com/read/29325900/orexin-b-modulates-synaptic-transmission-of-rod-bipolar-cells-in-rat-retina
#18
Gong Zhang, Xiao-Hua Wu, Guo-Zhong Xu, Shi-Jun Weng, Xiong-Li Yang, Yong-Mei Zhong
Orexin-A, -B play a crucial role in arousal and feeding by activating two G-protein-coupled receptors: orexin receptor 1 (OX1 R) and orexin receptor 2 (OX2 R). Orexins, along with orexin receptors, are expressed in retinal neurons, and they have been shown to differentially modulate excitatory AMPA receptors of amacrine and ganglion cells in the inner retina. In this work we report that orexin-B modulates the activity of rod bipolar cells (RBCs) located in the outer retina of rat. Intravitreal injection of orexin-B increased the amplitude of the scotopic electroretinographic b-wave, a reflection of RBC activity, recorded in vivo...
May 1, 2018: Neuropharmacology
https://www.readbyqxmd.com/read/29324898/the-etomidate-analog-et-26-hcl-retains-superior-myocardial-performance-comparisons-with-etomidate-in-vivo-and-in-vitro
#19
COMPARATIVE STUDY
Xingxing Liu, Haibo Song, Jun Yang, Cheng Zhou, Yi Kang, Linghui Yang, Jin Liu, Wensheng Zhang
OBJECTIVE: (R)-2-methoxyethyl1-(1-phenylethyl)-1H-imidazole-5-carboxylate hydrochloride (ET-26 HCl) is a novel etomidate analogue. The purpose of this study was to characterize whether ET-26 HCl could retain the superior myocardial performance of etomidate in vivo and in vitro. METHODS: In vivo, the influence of ET-26 HCl and etomidate on the cardiac function of dogs was confirmed using echocardiography and electrocardiogram. In vitro, a Langendorff preparation was used to examine direct myocardial performance in isolated rat hearts, and a whole-cell patch-clamp technique was used to study effects on the human ether-a-go-go-related gene (hERG) channel...
2018: PloS One
https://www.readbyqxmd.com/read/29297466/multi-neuron-intracellular-recording-in-vivo-via-interacting-autopatching-robots
#20
Suhasa B Kodandaramaiah, Francisco J Flores, Gregory L Holst, Annabelle C Singer, Xue Han, Emery N Brown, Edward S Boyden, Craig R Forest
The activities of groups of neurons in a circuit or brain region are important for neuronal computations that contribute to behaviors and disease states. Traditional extracellular recordings have been powerful and scalable, but much less is known about the intracellular processes that lead to spiking activity. We present a robotic system, the multipatcher, capable of automatically obtaining blind whole-cell patch clamp recordings from multiple neurons simultaneously. The multipatcher significantly extends automated patch clamping, or 'autopatching', to guide four interacting electrodes in a coordinated fashion, avoiding mechanical coupling in the brain...
January 3, 2018: ELife
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