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In vivo patch clamp recording

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https://www.readbyqxmd.com/read/28664138/naringenin-inhibits-pacemaking-activity-in-interstitial-cells-of-cajal-from-murine-small-intestine
#1
Hyun Jung Kim, Byung Joo Kim
BACKGROUND: Naringenin (NRG) is a common dietary polyphenolic constituent of fruits. NRG has diverse pharmacological activities, and is used in traditional medicine to treat various diseases including gastrointestinal (GI) disorders. Interstitial cells of Cajal (ICCs) are pacemaker cells of the GI tract. In this study, the authors investigated the effects of NRG on ICCs and on GI motility in vitro and in vivo. METHODS: ICCs were dissociated from mouse small intestines by enzymatic digestion...
June 2017: Integrative medicine research
https://www.readbyqxmd.com/read/28660248/optogenetic-evidence-for-a-direct-circuit-linking-nociceptive-transmission-through-the-parabrachial-complex-with-pain-modulating-neurons-of-the-rostral-ventromedial-medulla-rvm
#2
QiLiang Chen, Zachary Roeder, Ming-Hua Li, YangMiao Zhang, Susan L Ingram, Mary M Heinricher
The parabrachial complex (PB) is a functionally and anatomically complex structure involved in a range of homeostatic and sensory functions, including nociceptive transmission. There is also evidence that PB can engage descending pain-modulating systems, the best characterized of which is the rostral ventromedial medulla (RVM). Two distinct classes of RVM neurons, "ON-cells" and "OFF-cells," exert net pronociceptive and anti-nociceptive effects, respectively. PB was recently shown to be a relay of nociceptive information to RVM ON- and OFF-cells...
May 2017: ENeuro
https://www.readbyqxmd.com/read/28655649/interregional-electro-mechanical-heterogeneity-in-the-rabbit-myocardium
#3
REVIEW
Franz F Dressler, Ilona Bodi, Marius Menza, Robin Moss, Heiko Bugger, Christoph Bode, Jan C Behrends, Gunnar Seemann, Katja E Odening
BACKGROUND: Increased electrical heterogeneity has been causatively linked to arrhythmic disorders, yet the knowledge about physiological heterogeneity remains incomplete. This study investigates regional electro-mechanical heterogeneities in rabbits, one of the key animal models for arrhythmic disorders. METHODS AND FINDINGS: 7 wild-type rabbits were examined by phase-contrast magnetic resonance imaging in vivo to assess cardiac wall movement velocities. Using a novel data-processing algorithm regional contraction-like profiles were calculated...
June 24, 2017: Progress in Biophysics and Molecular Biology
https://www.readbyqxmd.com/read/28655644/pregnenolone-does-not-interfere-with-the-effects-of-cannabinoids-on-synaptic-transmission-in-the-cerebellum-and-the-nucleus-accumbens
#4
Anna Krohmer, Martin Brehm, Volker Auwärter, Bela Szabo
The steroid hormone pregnenolone attenuates several in vivo behavioural and somatic effects of the phytocannabinoid Δ(9)-tetrahydrocannabinol, and it was suggested that pregnenolone can protect the brain from cannabis intoxication. The primary neuronal cannabinoid action behind most of the behavioural and somatic effects of cannabinoids is presynaptic inhibition of synaptic transmission. Therefore, the hypothesis of the present study was that pregnenolone attenuates the inhibition of synaptic transmission elicited by cannabinoids...
June 24, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/28640864/targeting-breast-cancer-cells-by-mrs1477-a-positive-allosteric-modulator-of-trpv1-channels
#5
Mustafa Nazıroğlu, Bilal Çiğ, Walter Blum, Csaba Vizler, Andrea Buhala, Annamária Marton, Róbert Katona, Katalin Jósvay, Beat Schwaller, Zoltán Oláh, László Pecze
There is convincing epidemiological and experimental evidence that capsaicin, a potent natural transient receptor potential cation channel vanilloid member 1 (TRPV1) agonist, has anticancer activity. However, capsaicin cannot be given systemically in large doses, because of its induction of acute pain and neurological inflammation. MRS1477, a dihydropyridine derivative acts as a positive allosteric modulator of TRPV1, if added together with capsaicin, but is ineffective, if given alone. Addition of MRS1477 evoked Ca2+ signals in MCF7 breast cancer cells, but not in primary breast epithelial cells...
2017: PloS One
https://www.readbyqxmd.com/read/28592699/lower-affinity-of-isradipine-for-l-type-ca-2-channels-during-substantia-nigra-dopamine-neuron-like-activity-implications-for-neuroprotection-in-parkinson-s-disease
#6
Nadine J Ortner, Gabriella Bock, Antonios Dougalis, Maria Kharitonova, Johanna Duda, Simon Hess, Petronel Tuluc, Thomas Pomberger, Nadia Stefanova, Florian Pitterl, Thomas Ciossek, Herbert Oberacher, Henning J Draheim, Peter Kloppenburg, Birgit Liss, Jörg Striessnig
Ca(2+)-influx through L-type Ca(2+)-channels (LTCCs) is associated with activity-related stressful oscillations of Ca(2+) levels within dopaminergic (DA) neurons in the substantia nigra (SN), which may contribute to their selective degeneration in Parkinson's disease (PD). LTCC blockers were neuroprotective in mouse neurotoxin models of PD, and isradipine is currently undergoing testing in a phase III clinical trial in early PD. We report no evidence for neuroprotection by in vivo pretreatment with therapeutically relevant isradipine plasma levels, or Cav1...
July 12, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28592685/robotic-navigation-to-sub-cortical-neural-tissue-for-intracellular-electrophysiology-in-vivo
#7
William Andrew Stoy, Ilya Kolb, Gregory Holst, Yi J Liew, Aurelie Pala, Bo Yang, Edward S Boyden, Garrett B Stanley, Craig R Forest
In vivo studies of neurophysiology using the whole-cell patch clamp technique enable exquisite access to both intracellular dynamics and cytosol of cells in the living brain but are underrepresented in deep subcortical nuclei due to fouling of the sensitive electrode tip. We have developed an autonomous method to navigate electrodes around obstacles such as blood vessels, after identifying them as a source of contamination during regional pipette localization (RPL) in vivo. In mice, robotic navigation prevented fouling of the electrode tip, increasing RPL success probability 3 mm below the pial surface to 82% (n=72/88) over traditional, linear localization (25%, n=24/95) and resulted in high quality thalamic whole-cell recordings with average access resistance (32...
June 7, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28591742/andrographolide-inhibits-arrhythmias-and-is-cardioprotective-in-rabbits
#8
Mengliu Zeng, Wanzhen Jiang, Youjia Tian, Jie Hao, Zhenzhen Cao, Zhipei Liu, Chen Fu, Peihua Zhang, Jihua Ma
Andrographolide has a protective effect on the cardiovascular system. To study its cardic-electrophysiological effects, action potentials and voltage-gated Na+ (INa), Ca2+ (ICaL), and K+ (IK1, IKr, Ito and IKur) currents were recorded using whole-cell patch clamp and current clamp techniques. Additionally, the effects of andrographolide on aconitine-induced arrhythmias were assessed on electrocardiograms in vivo. We found that andrographolide shortened action potential duration and reduced maximum upstroke velocity in rabbit left ventricular and left atrial myocytes...
May 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28576617/synthesis-of-resveratrol-derivatives-as-new-analgesic-drugs-through-desensitization-of-the-trpa1-receptor
#9
Syuhei Nakao, Miyuki Mabuchi, Shenglan Wang, Yoko Kogure, Tadashi Shimizu, Koichi Noguchi, Akito Tanaka, Yi Dai
A series of 31 resveratrol derivatives was designed, synthesized and evaluated for activation and inhibition of the TRPA1 channel. Most acted as activators and desensitizers of TRPA1 channels like resveratrol or allyl isothiocyanate (AITC). Compound 4z (HUHS029) exhibited higher inhibitory activity than resveratrol with an IC50 value of 16.1μM. The activity of 4z on TRPA1 was confirmed in TRPA1-expressing HEK293 cells, as well as in rat dorsal root ganglia neurons by a whole cell patch clamp recording. Furthermore, pretreatment with 4z exhibited an analgesic effect on AITC-evoked TRPA1-related pain behavior in vivo...
May 22, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28566460/mesoscale-duration-activated-states-gate-spiking-in-response-to-fast-rises-in-membrane-voltage-in-the-awake-brain
#10
Annabelle C Singer, Giovanni Talei Franzesi, Suhasa B Kodandaramaiah, Francisco J Flores, Jeremy D Cohen, Albert K Lee, Christoph Börgers, Craig R Forest, Nancy J Kopell, Edward S Boyden
Seconds-scale network states, affecting many neurons within a network, modulate neural activity by complementing fast integration of neuron-specific inputs that arrive in the milliseconds before spiking. Non-rhythmic subthreshold dynamics at intermediate timescales, however, are less well-characterized. We found, using automated whole cell patch clamping in vivo, that spikes recorded in CA1 and barrel cortex in awake mice are often preceded not only by monotonic voltage rises lasting milliseconds, but also by more gradual (lasting 10s-100s of ms) depolarizations...
May 31, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28544556/electrophysiological-analysis-of-synaptic-transmission-in-drosophila
#11
REVIEW
Maria Bykhovskaia, Alexander Vasin
Synaptic transmission is dynamic, plastic, and highly regulated. Drosophila is an advantageous model system for genetic and molecular studies of presynaptic and postsynaptic mechanisms and plasticity. Electrical recordings of synaptic responses represent a wide-spread approach to study neuronal signaling and synaptic transmission. We discuss experimental techniques that allow monitoring synaptic transmission in Drosophila neuromuscular and central systems. Recordings of synaptic potentials or currents at the larval neuromuscular junction (NMJ) are most common and provide numerous technical advantages due to robustness of the preparation, large and identifiable muscles, and synaptic boutons which can be readily visualized...
May 24, 2017: Wiley Interdisciplinary Reviews. Developmental Biology
https://www.readbyqxmd.com/read/28542001/acetaminophen-metabolite-n-acylphenolamine-induces-analgesia-via-transient-receptor-potential-vanilloid-1-receptors-expressed-on-the-primary-afferent-terminals-of-c-fibers-in-the-spinal-dorsal-horn
#12
Nobuko Ohashi, Daisuke Uta, Mika Sasaki, Masayuki Ohashi, Yoshinori Kamiya, Tatsuro Kohno
BACKGROUND: The widely used analgesic acetaminophen is metabolized to N-acylphenolamine, which induces analgesia by acting directly on transient receptor potential vanilloid 1 or cannabinoid 1 receptors in the brain. Although these receptors are also abundant in the spinal cord, no previous studies have reported analgesic effects of acetaminophen or N-acylphenolamine mediated by the spinal cord dorsal horn. We hypothesized that clinical doses of acetaminophen induce analgesia via these spinal mechanisms...
August 2017: Anesthesiology
https://www.readbyqxmd.com/read/28496108/alpha7-nicotinic-acetylcholine-receptor-agonist-promotes-retinal-ganglion-cell-function-via-modulating-gabaergic-presynaptic-activity-in-a-chronic-glaucomatous-model
#13
Xujiao Zhou, Yun Cheng, Rong Zhang, Gang Li, Boqi Yang, Shenghai Zhang, Jihong Wu
Alpha-7 nicotinic acetylcholine receptor (α7-nAChR) agonists can prevent glutamate-induced excitotoxicity in cultured retinal ganglion cells (RGCs). However, the neuroprotective effects and the mechanism of action of PNU-282987, an α7-nAChR agonist, in a chronic in vivo rat glaucoma model are poorly understood. We found that elevated intraocular pressure (IOP) downregulated retinal α7-nAChR expression. Electroretinography revealed that the amplitude of the photopic negative response (PhNR) decreased in parallel with the loss of RGCs caused by elevated IOP...
May 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28456794/synergistic-inhibition-of-delayed-rectifier-k-and-voltage-gated-na-currents-by-artemisinin-in-pituitary-tumor-gh3-cells
#14
Edmund Cheung So, Sheng-Nan Wu, Ping-Ching Wu, Hui-Zhen Chen, Chia-Jung Yang
BACKGROUND: Artemisinin (ART) is an anti-malarial agent reported to influence endocrine function. METHODS: Effects of ART on ionic currents and action potentials (APs) in pituitary tumor (GH3) cells were evaluated by patch clamp techniques. RESULTS: ART inhibited the amplitude of delayed-rectifier K+ current (IK(DR)) in response to membrane depolarization and accelerated the process of current inactivation. It exerted an inhibitory effect on IK(DR) with an IC50 value of 11...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28435092/-terpinen-4-ol-changes-intracellular-ca-2-handling-and-induces-pacing-disturbance-in-rat-hearts
#15
Antonio Nei Santana Gondim, Aline Lara, Artur Santos-Miranda, Danilo Roman-Campos, Sandra Lauton-Santos, José Evaldo Rodrigues Menezes-Filho, Carla Maria Lins de Vasconcelos, Eduardo Antonio Conde-Garcia, Silvia Guatimosim, Jader S Cruz
(-)-Terpinen-4-ol is a naturally occurring plant monoterpene and has been shown to have a plethora of biological activities. The objective of this study was to investigate the effects of (-)-terpinen-4-ol on the rat heart, a key player in the control and maintenance of arterial blood pressure. The effects of (-)-terpinen-4-ol on the rat heart were investigated using isolated left atrium isometric force measurements, in vivo electrocardiogram (ECG) recordings, patch clamp technique, and confocal microscopy. It was observed that (-)-terpinen-4-ol reduced contraction force in an isolated left atrium at millimolar concentrations...
July 15, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28416816/scalable-electrophysiology-in-intact-small-animals-with-nanoscale-suspended-electrode-arrays
#16
Daniel L Gonzales, Krishna N Badhiwala, Daniel G Vercosa, Benjamin W Avants, Zheng Liu, Weiwei Zhong, Jacob T Robinson
Electrical measurements from large populations of animals would help reveal fundamental properties of the nervous system and neurological diseases. Small invertebrates are ideal for these large-scale studies; however, patch-clamp electrophysiology in microscopic animals typically requires invasive dissections and is low-throughput. To overcome these limitations, we present nano-SPEARs: suspended electrodes integrated into a scalable microfluidic device. Using this technology, we have made the first extracellular recordings of body-wall muscle electrophysiology inside an intact roundworm, Caenorhabditis elegans...
July 2017: Nature Nanotechnology
https://www.readbyqxmd.com/read/28415676/nitrobenzylthioinosine-mimics-adenosine-to-attenuate-the-epileptiform-discharge-of-hippocampal-neurons-from-epileptic-rats
#17
Hao Huang, Jing Wang, Jun Zhang, Zhong Luo, Dongxu Li, Xiaowei Qiu, Yan Peng, Zhongxiang Xu, Ping Xu, Zucai Xu
Nitrobenzylthioinosine (NBTI), a specific inhibitor of type 1 equilibrative nucleoside transporter, could regulate the extracellular adenosine concentration and have protective roles in seizures. However, the protection mechanism of NBTI in seizures remains poorly understood. Here, the expression pattern and subcellular distribution of adenosine A1 receptor were detected by Western blot analysis and double-labeling immunofluorescence staining in Lithium Chloride-Pilocarpine induced epileptic rat model. At 24 h after pilocarpine induced rat seizures, hippocampal slices were prepared and the evoked excitatory postsynaptic currents (eEPSCs) amplitude of pyramidal neurons in hippocampus CA1 region was recorded using whole-cell patch clamp...
May 30, 2017: Oncotarget
https://www.readbyqxmd.com/read/28394013/intermittent-theta-burst-stimulation-rescues-dopamine-dependent-corticostriatal-synaptic-plasticity-and-motor-behavior-in-experimental-parkinsonism-possible-role-of-glial-activity
#18
Fabrizio Cacace, Desirèe Mineo, Maria Teresa Viscomi, Emanuele Claudio Latagliata, Maria Mancini, Valeria Sasso, Anna Vannelli, Tiziana Pascucci, Valentina Pendolino, Elena Marcello, Silvia Pelucchi, Stefano Puglisi-Allegra, Marco Molinari, Barbara Picconi, Paolo Calabresi, Veronica Ghiglieri
BACKGROUND: Recent studies support the therapeutic utility of repetitive transcranial magnetic stimulation in Parkinson's disease (PD), whose progression is correlated with loss of corticostriatal long-term potentiation and long-term depression. Glial cell activation is also a feature of PD that is gaining increasing attention in the field because astrocytes play a role in chronic neuroinflammatory responses but are also able to manage dopamine (DA) levels. METHODS: Intermittent theta-burst stimulation protocol was applied to study the effect of therapeutic neuromodulation on striatal DA levels measured by means of in vivo microdialysis in 6-hydroxydopamine-hemilesioned rats...
July 2017: Movement Disorders: Official Journal of the Movement Disorder Society
https://www.readbyqxmd.com/read/28373496/in-vivo-patch-clamp-recording-in-awake-head-fixed-rodents
#19
Doyun Lee, Albert K Lee
Whole-cell recording has been used to measure and manipulate a neuron's spiking and subthreshold membrane potential, allowing assessment of the cell's inputs and outputs as well as its intrinsic membrane properties. This technique has also been combined with pharmacology and optogenetics as well as morphological reconstruction to address critical questions concerning neuronal integration, plasticity, and connectivity. This protocol describes a technique for obtaining whole-cell recordings in awake head-fixed animals, allowing such questions to be investigated within the context of an intact network and natural behavioral states...
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28348135/bk-channels-mediate-synaptic-plasticity-underlying-habituation-in-rats
#20
Tariq Zaman, Cleusa De Oliveira, Mahabba Smoka, Chakravarthi Narla, Michael O Poulter, Susanne Schmid
Habituation is a basic form of implicit learning and represents a sensory filter that is disrupted in autism, schizophrenia, and several other mental disorders. Despite extensive research in the past decades on habituation of startle and other escape responses, the underlying neural mechanisms are still not fully understood. There is evidence from previous studies indicating that BK channels might pay a critical role in habituation. We here used a wide array of approaches to test this hypothesis. We show that BK channel activation and subsequent phosphorylation of these channels are essential for synaptic depression presumably underlying startle habituation in rats, using patch-clamp recordings and voltage-sensitive dye imaging in slices...
March 27, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
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