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intermediate progenitor cell

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https://www.readbyqxmd.com/read/29764857/lineage-guided-notch-dependent-gliogenesis-by-drosophila-multi-potent-progenitors
#1
Qingzhong Ren, Takeshi Awasaki, Yu-Chun Wang, Yu-Fen Huang, Tzumin Lee
Macroglial cells in the central nervous system exhibit regional specialization and carry out region-specific functions. Diverse glial cells arise from specific progenitors in specific spatiotemporal patterns. This raises an interesting possibility that there exist glial precursors with distinct developmental fates, which govern region-specific gliogenesis. Here we mapped the glial progeny produced by the Drosophila type II neuroblasts, which, like vertebrate radial glia cells, yield both neurons and glia via intermediate neural progenitors (INPs)...
May 15, 2018: Development
https://www.readbyqxmd.com/read/29751817/tcf7l2-is-essential-for-neurogenesis-in-the-developing-mouse-neocortex
#2
Olga Chodelkova, Jan Masek, Vladimir Korinek, Zbynek Kozmik, Ondrej Machon
Generation of neurons in the embryonic neocortex is a balanced process of proliferation and differentiation of neuronal progenitor cells. Canonical Wnt signalling is crucial for expansion of radial glial cells in the ventricular zone and for differentiation of intermediate progenitors in the subventricular zone. We detected abundant expression of two transcrtiption factors mediating canonical Wnt signalling, Tcf7L1 and Tcf7L2, in the ventricular zone of the embryonic neocortex. Conditional knock-out analysis showed that Tcf7L2, but not Tcf7L1, is the principal Wnt mediator important for maintenance of progenitor cell identity in the ventricular zone...
May 11, 2018: Neural Development
https://www.readbyqxmd.com/read/29751772/expression-profiling-of-cell-intrinsic-regulators-in-the-process-of-differentiation-of-human-ipscs-into-retinal-lineages
#3
Jen-Hua Chuang, Aliaksandr A Yarmishyn, De-Kuang Hwang, Chih-Chien Hsu, Mong-Lien Wang, Yi-Ping Yang, Ke-Hung Chien, Shih-Hwa Chiou, Chi-Hsien Peng, Shih-Jen Chen
BACKGROUND: Differentiation of human induced pluripotent stem cells (hiPSCs) into retinal lineages offers great potential for medical application. Therefore, it is of crucial importance to know the key intrinsic regulators of differentiation and the specific biomarker signatures of cell lineages. METHODS: In this study, we used microarrays to analyze transcriptomes of terminally differentiated retinal ganglion cell (RGC) and retinal pigment epithelium (RPE) lineages, as well as intermediate retinal progenitor cells of optic vesicles (OVs) derived from hiPSCs...
May 11, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29750821/generation-of-hepatocyte-and-endocrine-pancreatic-like-cells-from-human-induced-endodermal-progenitor-cells
#4
Rangarajan Sambathkumar, Renate Akkerman, Sumitava Dastidar, Philip Roelandt, Manoj Kumar, Manmohan Bajaj, Ana Rita Mestre Rosa, Nicky Helsen, Veerle Vanslembrouck, Eric Kalo, Satish Khurana, Jos Laureys, Conny Gysemans, Marijke M Faas, Paul de Vos, Catherine M Verfaillie
Multipotent Adult Progenitor Cells (MAPCs) are one potential stem cell source to generate functional hepatocytes or β-cells. However, human MAPCs have less plasticity than pluripotent stem cells (PSCs), as their ability to generate endodermal cells is not robust. Here we studied the role of 14 transcription factors (TFs) in reprogramming MAPCs to induced endodermal progenitor cells (iENDO cells), defined as cells that can be long-term expanded and differentiated to both hepatocyte- and endocrine pancreatic-like cells...
2018: PloS One
https://www.readbyqxmd.com/read/29740158/single-cell-sequencing-reveals-the-origin-and-the-order-of-mutation-acquisition-in-t-cell-acute-lymphoblastic-leukemia
#5
Jolien De Bie, Sofie Demeyer, Llucia Alberti-Servera, Ellen Geerdens, Heidi Segers, Michaël Broux, Kim De Keersmaecker, Lucienne Michaux, Peter Vandenberghe, Thierry Voet, Nancy Boeckx, Anne Uyttebroeck, Jan Cools
Next-generation sequencing has provided a detailed overview of the various genomic lesions implicated in the pathogenesis of T-cell acute lymphoblastic leukemia (T-ALL). Typically, 10-20 protein-altering lesions are found in T-ALL cells at diagnosis. However, it is currently unclear in which order these mutations are acquired and in which progenitor cells this is initiated. To address these questions, we used targeted single-cell sequencing of total bone marrow cells and CD34+ CD38- multipotent progenitor cells for four T-ALL cases...
April 18, 2018: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
https://www.readbyqxmd.com/read/29713661/single-cell-computational-strategies-for-lineage-reconstruction-in-tissue-systems
#6
REVIEW
Charles A Herring, Bob Chen, Eliot T McKinley, Ken S Lau
Function at the organ level manifests itself from a heterogeneous collection of cell types. Cellular heterogeneity emerges from developmental processes by which multipotent progenitor cells make fate decisions and transition to specific cell types through intermediate cell states. Although genetic experimental strategies such as lineage tracing have provided insights into cell lineages, recent developments in single-cell technologies have greatly increased our ability to interrogate distinct cell types, as well as transitional cell states in tissue systems...
2018: Cellular and Molecular Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/29698519/the-loss-of-the-kinases-sada-and-sadb-results-in-early-neuronal-apoptosis-and-a-reduced-number-of-progenitors
#7
Pratibha Dhumale, Sindhu Menon, Joanna Chiang, Andreas W Püschel
The neurons that form the mammalian neocortex originate from progenitor cells in the ventricular (VZ) and subventricular zone (SVZ). Newborn neurons are multipolar but become bipolar during their migration from the germinal layers to the cortical plate (CP) by forming a leading process and an axon that extends in the intermediate zone (IZ). Once they settle in the CP, neurons assume a highly polarized morphology with a single axon and multiple dendrites. The AMPK-related kinases SadA and SadB are intrinsic factors that are essential for axon formation during neuronal development downstream of Lkb1...
2018: PloS One
https://www.readbyqxmd.com/read/29689192/hippo-signaling-plays-an-essential-role-in-cell-state-transitions-during-cardiac-fibroblast-development
#8
Yang Xiao, Matthew C Hill, Min Zhang, Thomas J Martin, Yuka Morikawa, Suya Wang, Alexander R Moise, Joshua D Wythe, James F Martin
During development, progenitors progress through transition states. The cardiac epicardium contains progenitors of essential non-cardiomyocytes. The Hippo pathway, a kinase cascade that inhibits the Yap transcriptional co-factor, controls organ size in developing hearts. Here, we investigated Hippo kinases Lats1 and Lats2 in epicardial diversification. Epicardial-specific deletion of Lats1/2 was embryonic lethal, and mutant embryos had defective coronary vasculature remodeling. Single-cell RNA sequencing revealed that Lats1/2 mutant cells failed to activate fibroblast differentiation but remained in an intermediate cell state with both epicardial and fibroblast characteristics...
April 23, 2018: Developmental Cell
https://www.readbyqxmd.com/read/29686651/sex-steroids-and-adult-neurogenesis-in-the-ventricular-subventricular-zone
#9
REVIEW
Giovanna Ponti, Alice Farinetti, Marilena Marraudino, GianCarlo Panzica, Stefano Gotti
The forebrain ventricular-subventricular zone (V-SVZ) continuously generates new neurons throughout life. Neural stem cells (type B1 cells) along the lateral ventricle become activated, self-renew, and give rise to proliferating precursors which progress along the neurogenic lineage from intermediate progenitors (type C cells) to neuroblasts (type A cells). Neuroblasts proliferate and migrate into the olfactory bulb and differentiate into different interneuronal types. Multiple factors regulate each step of this process...
2018: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29684005/in-vivo-clonal-analysis-reveals-spatiotemporal-regulation-of-thalamic-nucleogenesis
#10
Samuel Z H Wong, Earl Parker Scott, Wenhui Mu, Xize Guo, Ella Borgenheimer, Madeline Freeman, Guo-Li Ming, Qing-Feng Wu, Hongjun Song, Yasushi Nakagawa
The thalamus, a crucial regulator of cortical functions, is composed of many nuclei arranged in a spatially complex pattern. Thalamic neurogenesis occurs over a short period during mammalian embryonic development. These features have hampered the effort to understand how regionalization, cell divisions, and fate specification are coordinated and produce a wide array of nuclei that exhibit distinct patterns of gene expression and functions. Here, we performed in vivo clonal analysis to track the divisions of individual progenitor cells and spatial allocation of their progeny in the developing mouse thalamus...
April 23, 2018: PLoS Biology
https://www.readbyqxmd.com/read/29672621/imbalance-between-endothelial-damage-and-repair-capacity-in-chronic-obstructive-pulmonary-disease
#11
Jéssica García-Lucio, Victor I Peinado, Lluís de Jover, Roberto Del Pozo, Isabel Blanco, Cristina Bonjoch, Núria Coll-Bonfill, Tanja Paul, Olga Tura-Ceide, Joan Albert Barberà
BACKGROUND: Circulating endothelial microparticles (EMPs) and progenitor cells (PCs) are biological markers of endothelial function and endogenous repair capacity. The study was aimed to investigate whether COPD patients have an imbalance between EMPs to PCs compared to controls and to evaluate the effect of cigarette smoke on these circulating markers. METHODS: Circulating EMPs and PCs were determined by flow cytometry in 27 nonsmokers, 20 smokers and 61 COPD patients with moderate to severe airflow obstruction...
2018: PloS One
https://www.readbyqxmd.com/read/29658164/bipotent-megakaryocytic-erythroid-progenitors-concepts-and-controversies
#12
REVIEW
Juliana Xavier-Ferrucio, Diane S Krause
Hematopoietic stem and progenitor cells maintain blood formation throughout our lifetime by undergoing long and short-term self-renewal, respectively. As progenitor cells progress through the hematopoiesis process, their differentiation capabilities narrow, and at the point where potential limits the fate, and the precursors become committed to only one or two lineages. This review focuses on recent advances in the identification and characterization of bipotent megakaryocytic-erythroid progenitors (MEP), the cell that can further produce two completely different functional outputs: platelets and red blood cells...
April 15, 2018: Stem Cells
https://www.readbyqxmd.com/read/29614674/optical-depolarization-of-dcx-expressing-cells-promoted-cognitive-recovery-and-maturation-of-newborn-neurons-via-the-wnt-%C3%AE-catenin-pathway
#13
Ming-Liang Zhao, Shi-Jin Chen, Xiao-Hong Li, Li-Na Wang, Feng Chen, Shi-Jiang Zhong, Cheng Yang, Sheng-Kai Sun, Jian-Jun Li, Hua-Jiang Dong, Yue-Qing Dong, Yi Wang, Chong Chen
Electrical excitability by membrane depolarization is crucial for survival and maturation of newborn cells in the dentate gyrus of the hippocampus. However, traditional technology for membrane depolarization lacks temporal and spatial precision. Optogenetics can be used to activate channelrhodopsin-2 (ChR2), allowing cationic current to depolarize genetically targeted cells. In this study, we used ChR2-EGFP driven by doublecortin (DCX) to promote survival and maturation of newborn cells in the dentate gyrus after traumatic brain injury (TBI)...
March 28, 2018: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29574723/calcium-dependent-potassium-channels-control-proliferation-of-cardiac-progenitor-cells-and-bone-marrow-derived-mesenchymal-stem-cells
#14
Patrick Vigneault, Patrice Naud, Xiaoyan Qi, Jiening Xiao, Louis Villeneuve, Darryl R Davis, Stanley Nattel
Endogenous c-Kit+ cardiac progenitor cells (eCPCs) and bone marrow (BM)-derived mesenchymal stem cells (MSCs) are being developed for cardiac regenerative therapy, but a better understanding of their physiology is needed. Here, we addressed the unknown functional role of ion-channels in freshly-isolated eCPCs and expanded BM-MSCs using patch-clamp, microfluorometry and confocal microscopy. Isolated c-Kit+ eCPCs were purified from dog hearts by immunomagnetic selection. Ion-currents were barely detectable in freshly-isolated c-Kit+ eCPCs with intracellular calcium (Ca2+ i ) buffered...
March 25, 2018: Journal of Physiology
https://www.readbyqxmd.com/read/29570795/cortical-progenitor-biology-key-features-mediating-proliferation-versus-differentiation
#15
REVIEW
Ana Uzquiano, Ivan Gladwyn-Ng, Laurent Nguyen, Orly Reiner, Magdalena Götz, Fumio Matsuzaki, Fiona Francis
The cerebral cortex is a highly organized structure whose development depends on diverse progenitor cell types, namely apical radial glia, intermediate progenitors and basal radial glia cells, which are responsible for the production of the correct neuronal output. In recent years, these progenitor cell types have been deeply studied, particularly basal radial glial and their role in cortical expansion and gyrification. We review here a broad series of factors to regulate progenitor behavior and daughter cell fate...
March 23, 2018: Journal of Neurochemistry
https://www.readbyqxmd.com/read/29567672/origin-and-specification-of-type-ii-neuroblasts-in-the-drosophila-embryo
#16
José-Andrés Álvarez, Fernando J Díaz-Benjumea
In Drosophila , neural stem cells or neuroblasts (NBs) acquire different identities according to their site of origin in the embryonic neuroectoderm. Their identity determines the number of times they will divide and the types of daughter cells they will generate. All NBs divide asymmetrically, with type-I NBs undergoing self-renewal and generating another cell that will divide only once more. By contrast, a small set of NBs in the larval brain, type-II NBs, divide differently, undergoing self-renewal and generating an intermediate neural progenitor (INP) that continues to divide asymmetrically several more times, generating larger lineages...
March 22, 2018: Development
https://www.readbyqxmd.com/read/29555020/single-cell-transcriptomics-reveals-a-new-dynamical-function-of-transcription-factors-during-embryonic-hematopoiesis
#17
Isabelle Bergiers, Tallulah Andrews, Özge Vargel Bölükbaşı, Andreas Buness, Ewa Janosz, Natalia Lopez-Anguita, Kerstin Ganter, Kinga Kosim, Cemre Celen, Gülce Itır Perçin, Paul Collier, Bianka Baying, Vladimir Benes, Martin Hemberg, Christophe Lancrin
Recent advances in single-cell transcriptomics techniques have opened the door to the study of gene regulatory networks (GRNs) at the single-cell level. Here, we studied the GRNs controlling the emergence of hematopoietic stem and progenitor cells from mouse embryonic endothelium using a combination of single-cell transcriptome assays. We found that a heptad of transcription factors (Runx1, Gata2, Tal1, Fli1, Lyl1, Erg and Lmo2) is specifically co-expressed in an intermediate population expressing both endothelial and hematopoietic markers...
March 20, 2018: ELife
https://www.readbyqxmd.com/read/29545397/molecular-transitions-in-early-progenitors-during-human-cord-blood-hematopoiesis
#18
Shiwei Zheng, Efthymia Papalexi, Andrew Butler, William Stephenson, Rahul Satija
Hematopoietic stem cells (HSCs) give rise to diverse cell types in the blood system, yet our molecular understanding of the early trajectories that generate this enormous diversity in humans remains incomplete. Here, we leverage Drop-seq, a massively parallel single-cell RNA sequencing (scRNA-seq) approach, to individually profile 20,000 progenitor cells from human cord blood, without prior enrichment or depletion for individual lineages based on surface markers. Our data reveal a transcriptional compendium of progenitor states in human cord blood, representing four committed lineages downstream from HSC, alongside the transcriptional dynamics underlying fate commitment...
March 15, 2018: Molecular Systems Biology
https://www.readbyqxmd.com/read/29541793/nestin-expressing-progenitor-cells-function-identity-and-therapeutic-implications
#19
REVIEW
Aurora Bernal, Lorena Arranz
The neuroepithelial stem cell protein, or Nestin, is a cytoskeletal intermediate filament initially characterized in neural stem cells. However, current extensive evidence obtained in in vivo models and humans shows presence of Nestin+ cells with progenitor and/or regulatory functions in a number of additional tissues, remarkably bone marrow. This review presents the current knowledge on the role of Nestin in essential stem cell functions, including self-renewal/proliferation, differentiation and migration, in the context of the cytoskeleton...
March 14, 2018: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/29539641/a-single-cell-rna-seq-survey-of-the-developmental-landscape-of-the-human-prefrontal-cortex
#20
Suijuan Zhong, Shu Zhang, Xiaoying Fan, Qian Wu, Liying Yan, Ji Dong, Haofeng Zhang, Long Li, Le Sun, Na Pan, Xiaohui Xu, Fuchou Tang, Jun Zhang, Jie Qiao, Xiaoqun Wang
The mammalian prefrontal cortex comprises a set of highly specialized brain areas containing billions of cells and serves as the centre of the highest-order cognitive functions, such as memory, cognitive ability, decision-making and social behaviour. Although neural circuits are formed in the late stages of human embryonic development and even after birth, diverse classes of functional cells are generated and migrate to the appropriate locations earlier in development. Dysfunction of the prefrontal cortex contributes to cognitive deficits and the majority of neurodevelopmental disorders; there is therefore a need for detailed knowledge of the development of the prefrontal cortex...
March 14, 2018: Nature
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