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intermediate progenitor cell

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https://www.readbyqxmd.com/read/28285879/lineage-specification-from-prostate-progenitor-cells-requires-gata3-dependent-mitotic-spindle-orientation
#1
Maxwell E R Shafer, Alana H T Nguyen, Mathieu Tremblay, Sophie Viala, Mélanie Béland, Nicholas R Bertos, Morag Park, Maxime Bouchard
During prostate development, basal and luminal cell lineages are generated through symmetric and asymmetric divisions of bipotent basal cells. However, the extent to which spindle orientation controls division symmetry or cell fate, and the upstream factors regulating this process, are still elusive. We report that GATA3 is expressed in both prostate basal progenitor and luminal cells and that loss of GATA3 leads to a mislocalization of PRKCZ, resulting in mitotic spindle randomization during progenitor cell division...
March 7, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28253906/molecular-imaging-of-nestin-in-neuroinflammatory-conditions-reveals-marked-signal-induction-in-activated-microglia
#2
Senthil Krishnasamy, Yuan-Cheng Weng, Sai Sampath Thammisetty, Daniel Phaneuf, Melanie Lalancette-Hebert, Jasna Kriz
BACKGROUND: Nestin is a known marker of neuronal progenitor cells in the adult brain. Following neuro- and gliogenesis, nestin is replaced by cell type-specific intermediate filaments, e.g., neurofilaments for panneuronal expression and glial fibrillary acidic protein as a specific marker of mature astrocytes. While previous work have been mostly focused on the neuronal fate of nestin-positive progenitors, in the present study, we sought to investigate in real time how nestin signals and cellular expression patterns are controlled in the context of neuroinflammatory challenge and ischemic brain injury...
March 3, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28249158/ultrastructural-characterization-of-zika-virus-replication-factories
#3
Mirko Cortese, Sarah Goellner, Eliana Gisela Acosta, Christopher John Neufeldt, Olga Oleksiuk, Marko Lampe, Uta Haselmann, Charlotta Funaya, Nicole Schieber, Paolo Ronchi, Martin Schorb, Priit Pruunsild, Yannick Schwab, Laurent Chatel-Chaix, Alessia Ruggieri, Ralf Bartenschlager
A global concern has emerged with the pandemic spread of Zika virus (ZIKV) infections that can cause severe neurological symptoms in adults and newborns. ZIKV is a positive-strand RNA virus replicating in virus-induced membranous replication factories (RFs). Here we used various imaging techniques to investigate the ultrastructural details of ZIKV RFs and their relationship with host cell organelles. Analyses of human hepatic cells and neural progenitor cells infected with ZIKV revealed endoplasmic reticulum (ER) membrane invaginations containing pore-like openings toward the cytosol, reminiscent to RFs in Dengue virus-infected cells...
February 28, 2017: Cell Reports
https://www.readbyqxmd.com/read/28245922/an-hdac1-rpd3-poised-circuit-balances-continual-self-renewal-and-rapid-restriction-of-developmental-potential-during-asymmetric-stem-cell-division
#4
Derek H Janssens, Danielle C Hamm, Lucas Anhezini, Qi Xiao, Karsten H Siller, Sarah E Siegrist, Melissa M Harrison, Cheng-Yu Lee
How the developmental potential of differentiating stem cell progeny becomes rapidly and stably restricted following asymmetric stem cell division is unclear. In the fly larval brain, earmuff (erm) uniquely functions to restrict the developmental potential of intermediate neural progenitors (INPs) generated by asymmetrically dividing neural stem cells (neuroblasts). Here we demonstrate that the histone deacetylase Hdac1/Rpd3 functions together with self-renewal transcriptional repressors to maintain the erm immature INP enhancer in an inactive but poised state in neuroblasts...
February 27, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28235774/development-of-platelets-during-steady-state-and-inflammation
#5
REVIEW
Gerhard Müller-Newen, Matthias B Stope, Thomas Kraus, Patrick Ziegler
Megakaryocytes (MK) are the sole source of platelets in the body. They develop from lineage-committed hematopoietic stem and progenitor cells (HSPCs) via intermediate cells, which differ in morphology, size, ploidy, and surface phenotype. Development and maturation of MKs is governed by different transcription factors, including GATA-1, E26 transformation-specific transcription factor (ETS) family members, nuclear factor erythroid 2 transcription factor (NF-E2), and STAT3. During such challenges as acute inflammation, platelets are consumed in high numbers and must be replenished to secure survival of the host...
February 24, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28224273/over-expression-of-mir-196b-5p-is-significantly-associated-with-the-progression-of-myelodysplastic-syndrome
#6
Jing Wen, Ying Huang, Hongying Li, Xupai Zhang, Peng Cheng, Donghong Deng, Zhigang Peng, Jun Luo, Weihua Zhao, Yongrong Lai, Zhenfang Liu
Myelodysplastic syndrome (MDS) is a clonal stem cell disorder characterized by ineffective hematopoiesis with a high risk of transformation to acute myeloid leukemia (AML). miRNAs function as tumor suppressors and oncogenes in various cancers and regulate the differentiation potential of hematopoietic stem and progenitor cells (HSPCs). It has been suggested that miRNAs may play an important role in progression of MDS. We analyzed bone marrow samples collected from MDS patients according to different risk stratification indicated by the International Prognostic Scoring System (IPSS)...
February 21, 2017: International Journal of Hematology
https://www.readbyqxmd.com/read/28196691/healing-of-a-large-long-bone-defect-through-serum-free-in%C3%A2-vitro-priming-of%C3%A2-human-periosteum-derived-cells
#7
Johanna Bolander, Wei Ji, Jeroen Leijten, Liliana Moreira Teixeira, Veerle Bloemen, Dennis Lambrechts, Malay Chaklader, Frank P Luyten
Clinical translation of cell-based strategies for regenerative medicine demands predictable in vivo performance where the use of sera during in vitro preparation inherently limits the efficacy and reproducibility. Here, we present a bioinspired approach by serum-free pre-conditioning of human periosteum-derived cells, followed by their assembly into microaggregates simultaneously primed with bone morphogenetic protein 2 (BMP-2). Pre-conditioning resulted in a more potent progenitor cell population, while aggregation induced osteochondrogenic differentiation, further enhanced by BMP-2 stimulation...
March 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28183821/exercise-induced-upregulation-of-endothelial-adhesion-molecules-in-human-skeletal-muscle-and-number-of-circulating-cells-with-remodeling-properties
#8
Anna Strömberg, Eric Rullman, Eva Jansson, Thomas Gustafsson
Multipotent cells have received great interest due to their potential capacity to repair and remodel peripheral tissues. We examined the effect of an acute exercise bout on the number of circulating cells with known remodeling properties, and the level of factors in plasma and skeletal muscle tissue with potential to recruit these cells. Twenty healthy male subjects performed a 60 min cycling exercise. Blood samples for flow cytometry were drawn from 10 subjects (Group 1) before and up to 2 h after exercise, and absolute cell counts of the classical (CD14(++)CD16(-)), intermediate (CD14(++)CD16(+)), and nonclassical (CD14(+)CD16(++)) monocyte (MO) subpopulations, and of CD45(dim)CD34(+)VEGFR2(+) endothelial progenitor cells (EPCs) were measured using bead-based determination...
February 9, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28162994/thyroid-progenitors-are-robustly-derived-from-embryonic-stem-cells-through-transient-developmental-stage-specific-overexpression-of-nkx2-1
#9
Keri Dame, Steven Cincotta, Alex H Lang, Reeti M Sanghrajka, Liye Zhang, Jinyoung Choi, Letty Kwok, Talitha Wilson, Maciej M Kańduła, Stefano Monti, Anthony N Hollenberg, Pankaj Mehta, Darrell N Kotton, Laertis Ikonomou
The clinical importance of anterior foregut endoderm (AFE) derivatives, such as thyrocytes, has led to intense research efforts for their derivation through directed differentiation of pluripotent stem cells (PSCs). Here, we identify transient overexpression of the transcription factor (TF) NKX2-1 as a powerful inductive signal for the robust derivation of thyrocyte-like cells from mouse PSC-derived AFE. This effect is highly developmental stage specific and dependent on FOXA2 expression levels and precise modulation of BMP and FGF signaling...
February 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28117403/minimizing-the-risk-of-allo-sensitization-to-optimize-the-benefit-of-allogeneic-cardiac-derived-stem-progenitor-cells
#10
Hocine R Hocine, Hicham E L Costa, Noemie Dam, Jerome Giustiniani, Itziar Palacios, Pascale Loiseau, Armand Benssusan, Luis R Borlado, Dominique Charron, Caroline Suberbielle, Nabila Jabrane-Ferrat, Reem Al-Daccak
Allogeneic human cardiac-derived stem/progenitor cells (hCPC) are currently under clinical investigation for cardiac repair. While cellular immune response against allogeneic hCPC could be part of their beneficial-paracrine effects, their humoral immune response remains largely unexplored. Donor-specific HLA antibodies (DSA-HLA-I/DSA-HLA-II), primary elements of antibody-mediated allograft injury, might present an unidentified risk to allogeneic hCPC therapy. Here we established that the binding strength of anti-HLA monoclonal antibodies delineates hCPC proneness to antibody-mediated injury...
January 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28111078/role-of-mitochondrial-metabolism-in-the-control-of-early-lineage-progression-and-aging-phenotypes-in-adult-hippocampal-neurogenesis
#11
Ruth Beckervordersandforth, Birgit Ebert, Iris Schäffner, Jonathan Moss, Christian Fiebig, Jaehoon Shin, Darcie L Moore, Laboni Ghosh, Mariela F Trinchero, Carola Stockburger, Kristina Friedland, Kathrin Steib, Julia von Wittgenstein, Silke Keiner, Christoph Redecker, Sabine M Hölter, Wei Xiang, Wolfgang Wurst, Ravi Jagasia, Alejandro F Schinder, Guo-Li Ming, Nicolas Toni, Sebastian Jessberger, Hongjun Song, D Chichung Lie
Precise regulation of cellular metabolism is hypothesized to constitute a vital component of the developmental sequence underlying the life-long generation of hippocampal neurons from quiescent neural stem cells (NSCs). The identity of stage-specific metabolic programs and their impact on adult neurogenesis are largely unknown. We show that the adult hippocampal neurogenic lineage is critically dependent on the mitochondrial electron transport chain and oxidative phosphorylation machinery at the stage of the fast proliferating intermediate progenitor cell...
February 8, 2017: Neuron
https://www.readbyqxmd.com/read/28089668/ebio-does-not-induce-cardiomyogenesis-in-human-pluripotent-stem-cells-but-modulates-cardiac-subtype-enrichment-by-lineage-selective-survival
#12
Monica Jara-Avaca, Henning Kempf, Michael Rückert, Diana Robles-Diaz, Annika Franke, Jeanne de la Roche, Martin Fischer, Daniela Malan, Philipp Sasse, Wladimir Solodenko, Gerald Dräger, Andreas Kirschning, Ulrich Martin, Robert Zweigerdt
Subtype-specific human cardiomyocytes (CMs) are valuable for basic and applied research. Induction of cardiomyogenesis and enrichment of nodal-like CMs was described for mouse pluripotent stem cells (mPSCs) in response to 1-ethyl-2-benzimidazolinone (EBIO), a chemical modulator of small-/intermediate-conductance Ca(2+)-activated potassium channels (SKs 1-4). Investigating EBIO in human pluripotent stem cells (PSCs), we have applied three independent differentiation protocols of low to high cardiomyogenic efficiency...
February 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28064242/cyclin-dependent-kinase-5-activity-is-required-for-allogeneic-t-cell-responses-after-hematopoietic-cell-transplantation-in-mice
#13
David Askew, Tej K Pareek, Saada Eid, Sudipto Ganguly, Megan Tyler, Alex Y Huang, John J Letterio, Kenneth R Cooke
Molecular intermediates in T-cell activation pathways are crucial targets for the therapy and prevention of graft-versus-host disease (GVHD) following allogeneic hematopoietic cell transplantation (allo-HCT). We recently identified an essential role for cyclin-dependent kinase 5 (Cdk5) in T-cell activation and effector function, but the contribution of Cdk5 activity to the development of GVHD has not been explored. Using an established, preclinical, murine, GVHD model, we reveal that Cdk5 activity is increased in key target organs early after allo-HCT...
January 12, 2017: Blood
https://www.readbyqxmd.com/read/28057268/control-of-neuronal-development-by-t-box-genes-in-the-brain
#14
A B Mihalas, R F Hevner
T-box transcription factors play key roles in the regulation of developmental processes such as cell differentiation and migration. Mammals have 17 T-box genes, of which several regulate brain development. The Tbr1 subfamily of T-box genes is particularly important in development of the cerebral cortex, olfactory bulbs (OBs), and cerebellum. This subfamily is comprised of Tbr1, Tbr2 (also known as Eomes), and Tbx21. In developing cerebral cortex, Tbr2 and Tbr1 are expressed during successive stages of differentiation in the pyramidal neuron lineage, from Tbr2+ intermediate progenitors to Tbr1+ postmitotic glutamatergic neurons...
2017: Current Topics in Developmental Biology
https://www.readbyqxmd.com/read/28041877/cytosine-5-rna-methylation-regulates-neural-stem-cell-differentiation-and%C3%A2-motility
#15
Joana V Flores, Lucía Cordero-Espinoza, Feride Oeztuerk-Winder, Amanda Andersson-Rolf, Tommaso Selmi, Sandra Blanco, Jignesh Tailor, Sabine Dietmann, Michaela Frye
Loss-of-function mutations in the cytosine-5 RNA methylase NSUN2 cause neurodevelopmental disorders in humans, yet the underlying cellular processes leading to the symptoms that include microcephaly remain unclear. Here, we show that NSUN2 is expressed in early neuroepithelial progenitors of the developing human brain, and its expression is gradually reduced during differentiation of human neuroepithelial stem (NES) cells in vitro. In the developing Nsun2(-/-) mouse cerebral cortex, intermediate progenitors accumulate and upper-layer neurons decrease...
January 10, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28028137/diversification-of-cell-lineages-in-ureter-development
#16
Tobias Bohnenpoll, Sarah Feraric, Marvin Nattkemper, Anna-Carina Weiss, Carsten Rudat, Max Meuser, Mark-Oliver Trowe, Andreas Kispert
The mammalian ureter consists of a mesenchymal wall composed of smooth muscle cells and surrounding fibrocytes of the tunica adventitia and the lamina propria and an inner epithelial lining composed of layers of basal, intermediate, and superficial cells. How these cell types arise from multipotent progenitors is poorly understood. Here, we performed marker analysis, cell proliferation assays, and genetic lineage tracing to define the lineage relations and restrictions of the mesenchymal and epithelial cell types in the developing and mature mouse ureter...
December 27, 2016: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/28006658/direct-induction-of-neural-progenitor-cells-transiently-passes-through-a-partially-reprogrammed-state
#17
Rui Gao, Wenchao Xiu, Linfeng Zhang, Ruge Zang, Lei Yang, Chenfei Wang, Min Wang, Mingzhu Wang, Li Yi, Yuanyuan Tang, Yawei Gao, Hong Wang, Jiajie Xi, Wenqiang Liu, Yixuan Wang, Xuejun Wen, Yongchun Yu, Yong Zhang, Liang Chen, Jiayu Chen, Shaorong Gao
The generation of functional neural progenitor cells (NPCs) holds great promise for both research and clinical applications in neurodegenerative diseases. Traditionally, NPCs are derived from embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), or NPCs can be directly converted from somatic cells by sets of transcription factors or by a combination of chemical cocktails and/or hypoxia. However, the ethical issues of ESCs, the risk of tumorigenesis from iPSCs and transgenic integration from exogenous genes as well as complicated manipulation and time-consuming of chemical induced NPCs (ciNPCs) limit the applications of these strategies...
March 2017: Biomaterials
https://www.readbyqxmd.com/read/27974623/genetic-regulation-and-function-of-epidermal-growth-factor-receptor-signalling-in-patterning-of-the-embryonic-drosophila-brain
#18
David Jussen, Janina von Hilchen, Rolf Urbach
The specification of distinct neural cell types in central nervous system development crucially depends on positional cues conferred to neural stem cells in the neuroectoderm. Here, we investigate the regulation and function of the epidermal growth factor receptor (EGFR) signalling pathway in early development of the Drosophila brain. We find that localized EGFR signalling in the brain neuroectoderm relies on a neuromere-specific deployment of activating (Spitz, Vein) and inhibiting (Argos) ligands. Activated EGFR controls the spatially restricted expression of all dorsoventral (DV) patterning genes in a gene- and neuromere-specific manner...
December 2016: Open Biology
https://www.readbyqxmd.com/read/27965439/transcriptional-regulation-of-intermediate-progenitor-cell-generation-during-hippocampal-development
#19
Lachlan Harris, Oressia Zalucki, Ilan Gobius, Hannah McDonald, Jason Osinki, Tracey J Harvey, Alexandra Essebier, Diana Vidovic, Ivan Gladwyn-Ng, Thomas H Burne, Julian I Heng, Linda J Richards, Richard M Gronostajski, Michael Piper
During forebrain development, radial glia generate neurons through the production of intermediate progenitor cells (IPCs). The production of IPCs is a central tenet underlying the generation of the appropriate number of cortical neurons, but the transcriptional logic underpinning this process remains poorly defined. Here, we examined IPC production using mice lacking the transcription factor nuclear factor I/X (Nfix). We show that Nfix deficiency delays IPC production and prolongs the neurogenic window, resulting in an increased number of neurons in the postnatal forebrain...
December 15, 2016: Development
https://www.readbyqxmd.com/read/27955690/chd7-promotes-proliferation-of-neural-stem-cells-mediated-by-mif
#20
Shigeki Ohta, Tomonori Yaguchi, Hironobu Okuno, Hervé Chneiweiss, Yutaka Kawakami, Hideyuki Okano
Macrophage migration inhibitory factor (MIF) plays an important role in supporting the proliferation and/or survival of murine neural stem/progenitor cells (NSPCs); however, the downstream effectors of this factor remain unknown. Here, we show that MIF increases the expression of Pax6 and Chd7 in NSPCs in vitro. During neural development, the chromatin remodeling factor Chd7 (chromatin helicase-DNA-binding protein 7) is expressed in the ventricular zone of the telencephalon of mouse brain at embryonic day 14...
December 13, 2016: Molecular Brain
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