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https://www.readbyqxmd.com/read/28624368/conservation-of-bmp2-4-expression-patterns-within-the-clade-branchiostoma-amphioxus-resolving-interspecific-discrepancies
#1
Luok Wen Yong, Stéphanie Bertrand, Jr-Kai Yu, Hector Escriva, Nicholas D Holland
In 2016, Kaji et al. concluded that the amphioxus mouth has the quality of a coelomoduct and is, therefore, not homologous to the oral opening of any other animal. They studied a Japanese population of Branchiostoma japonicum and based their conclusion, in part, on the larval expression of BMP2/4 in cells that reportedly joined the rim of the forming mouth. They did not detect transcription of that gene in any other tissues in the anterior region of the larva. Their results were almost the inverse of findings for B...
June 15, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28497228/18-des-hydroxy-cytochalasin-an-antiparasitic-compound-of-diaporthe-phaseolorum-92c-an-endophytic-fungus-isolated-from-combretum-lanceolatum-pohl-ex-eichler
#2
Elson Rudimar Brissow, Igor Pereira da Silva, Kátia Aparecida de Siqueira, Jaqueline Alves Senabio, Leticia Pereira Pimenta, Ana Helena Januário, Lizandra Guidi Magalhães, Ricardo Andrade Furtado, Denise Crispim Tavares, Policarpo Ademar Sales Junior, Jane Lima Santos, Marcos Antônio Soares
Chemical investigation of the ethyl acetate extract from the endophytic fungus Diaporthe phaseolorum-92C (92C) isolated from the roots of Combretum lanceolatum led to the isolation of 18-des-hydroxy Cytochalasin H (compound 1). The trypanocidal and schistosomicidal activity and cytotoxicity of the extract from 92C were evaluated. The schistosomicidal, leishmanicidal, antimicrobial, and antioxidant actions, as well as the antitumor activity against the breast cancer cells MDA-MB-231 and MCF-7, and the cytotoxicity towards normal human lung fibroblasts GM07492A of compound 1 was tested...
July 2017: Parasitology Research
https://www.readbyqxmd.com/read/28415000/changes-in-copper-toxicity-towards-diatom-communities-with-experimental-warming
#3
Soizic Morin, Anne Sophie Lambert, Elena Planes Rodriguez, Aymeric Dabrin, Marina Coquery, Stephane Pesce
Biological communities in aquatic environments most commonly face multiple stress, where natural and anthropogenic stressors often act jointly. Their interactions are most easily assessed using short cycle organisms such as periphytic diatoms. In this experiment, we analyzed the combined effects of copper exposure and warming on diatom successions over 6 weeks. Natural biofilm collected in winter was left to grow in mesocosms exposed or unexposed to realistic Cu concentrations at four different temperatures...
April 6, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28103795/lineage-specific-duplication-of-amphioxus-retinoic-acid-degrading-enzymes-cyp26-resulted-in-sub-functionalization-of-patterning-and-homeostatic-roles
#4
João E Carvalho, Maria Theodosiou, Jie Chen, Pascale Chevret, Susana Alvarez, Angel R De Lera, Vincent Laudet, Jenifer C Croce, Michael Schubert
BACKGROUND: During embryogenesis, tight regulation of retinoic acid (RA) availability is fundamental for normal development. In parallel to RA synthesis, a negative feedback loop controlled by RA catabolizing enzymes of the cytochrome P450 subfamily 26 (CYP26) is crucial. In vertebrates, the functions of the three CYP26 enzymes (CYP26A1, CYP26B1, and CYP26C1) have been well characterized. By contrast, outside vertebrates, little is known about CYP26 complements and their biological roles...
January 19, 2017: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/27921244/towards-green-oviposition-deterrents-effectiveness-of-syzygium-lanceolatum-myrtaceae-essential-oil-against-six-mosquito-vectors-and-impact-on-four-aquatic-biological-control-agents
#5
Giovanni Benelli, Mohan Rajeswary, Marimuthu Govindarajan
Essential oils (EOs) from plants may be alternative sources of molecules toxic against mosquito vectors of public health relevance. Most of researches in this field focused on EOs as larvicides or ovicides, while limited efforts focused on the exploitation of EOs as oviposition deterrents. In the present study, the larvicidal and oviposition deterrent activity of Syzygium lanceolatum leaf EO was evaluated against six mosquito species, Anopheles stephensi, An. subpictus, Aedes aegypti, Ae. albopictus, Culex quinquefasciatus, and Cx...
December 5, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27917830/structural-analysis-of-the-bright-monomeric-yellow-green-fluorescent-protein-mneongreen-obtained-by-directed-evolution
#6
Damien Clavel, Guillaume Gotthard, David von Stetten, Daniele De Sanctis, Hélène Pasquier, Gerard G Lambert, Nathan C Shaner, Antoine Royant
Until recently, genes coding for homologues of the autofluorescent protein GFP had only been identified in marine organisms from the phyla Cnidaria and Arthropoda. New fluorescent-protein genes have now been found in the phylum Chordata, coding for particularly bright oligomeric fluorescent proteins such as the tetrameric yellow fluorescent protein lanYFP from Branchiostoma lanceolatum. A successful monomerization attempt led to the development of the bright yellow-green fluorescent protein mNeonGreen. The structures of lanYFP and mNeonGreen have been determined and compared in order to rationalize the directed evolution process leading from a bright, tetrameric to a still bright, monomeric fluorescent protein...
December 1, 2016: Acta Crystallographica. Section D, Structural Biology
https://www.readbyqxmd.com/read/27470795/the-millipede-genus-enghoffosoma-golovatch-1993-recorded-in-vietnam-br-for-the-first-time-with-descriptions-of-three-new-species-diplopoda-polydesmida-paradoxosomatidae
#7
Anh D Nguyen, Sergei I Golovatch
The genus Enghoffosoma is recorded from Vietnam for the first time, altogether with six species involved. Two species, E. lanceolatum Likhitrakarn et al., 2014 and E. anchoriforme Likhitrakarn et al., 2014, were previously known only from the western part of Laos, thus being new to the fauna of Vietnam. One more species, Sundanina fedorenkoi Golovatch, 2016, very recently described from Dak Lak Province, southern Vietnam, is formally transferred to Enghoffosoma, comb. nov., based both on a strict topotype and a sample from Lam Dong Province, southern Vietnam...
July 20, 2016: Zootaxa
https://www.readbyqxmd.com/read/27232128/suitability-of-monotypic-and-mixed-diets-for-anopheles-hermsi-larval-development
#8
Donald A Beasley, William E Walton
The developmental time and survival to eclosion of Anopheles hermsi Barr & Guptavanij fed monotypic and mixed diets of ten food types were examined in laboratory studies. Larvae fed monotypic diets containing animal detritus (freeze-dried rotifers, freeze-dried Daphnia pulicaria, and TetraMin® fish food flakes) and the mixotrophic protistan Cryptomonas ovata developed faster and survived better than larvae that were fed other monotypic diets. Survival to adulthood of larvae fed several concentrations of the diatom Planothidium (=Achnanthes) lanceolatum was poor (<13%) and larval development time was approximately twice that of larvae fed TetraMin® fish food flakes, the standard laboratory diet...
June 2016: Journal of Vector Ecology: Journal of the Society for Vector Ecology
https://www.readbyqxmd.com/read/27081350/taxonomy-of-atlantic-central-african-orchids-5-a-new-species-of-angraecum-sect-conchoglossum-orchidaceae-angraecinae-from-gabon-and-cameroon
#9
Vladimir Ječmenica, Vincent Droissart, Nausicaa Noret, Tariq Stévart
Recent field inventories and taxonomic research in Central Africa have resulted in the discovery of many new orchid species. Five specimens of an apparently new Angraecum species were collected in Gabon and Cameroon. They stand out for their hanging habit and short zig-zag stem. Morphology of leaves and habit is somewhat comparable to Angraecum cultriforme and Angraecum stolzii, two species from East Africa. Flowers of the novelty share the general morphology of Angraecum pyriforme from which the new species is distinguished by being smaller and with a different lip-spur ratio...
2016: PhytoKeys
https://www.readbyqxmd.com/read/26950610/tricyclic-acylphloroglucinols-from-hypericum-lanceolatum-and-regioselective-synthesis-of-selancins-a-and-b
#10
Serge A T Fobofou, Katrin Franke, Andrea Porzel, Wolfgang Brandt, Ludger A Wessjohann
The chemical investigation of the chloroform extract of Hypericum lanceolatum guided by (1)H NMR, ESIMS, and TLC profiles led to the isolation of 11 new tricyclic acylphloroglucinol derivatives, named selancins A-I (1-9) and hyperselancins A and B (10 and 11), along with the known compound 3-O-geranylemodin (12), which is described for a Hypericum species for the first time. Compounds 8 and 9 are the first examples of natural products with a 6-acyl-2,2-dimethylchroman-4-one core fused with a dimethylpyran unit...
April 22, 2016: Journal of Natural Products
https://www.readbyqxmd.com/read/26864726/combretum-lanceolatum-flowers-ethanol-extract-inhibits-hepatic-gluconeogenesis-an-in-vivo-mechanism-study
#11
COMPARATIVE STUDY
Juliany Torres Siqueira, Emanuele Batistela, Mayara Peron Pereira, Virginia Claudia da Silva, Paulo Teixeira de Sousa Junior, Claudia Marlise Balbinotti Andrade, Nair Honda Kawashita, Gisele Lopes Bertolini, Amanda Martins Baviera
Context Ethnopharmacological studies have demonstrated that plants of the Combretum genus presented antidiabetic activity, including Combretum lanceolatum Pohl ex Eichler (Combretaceae). Objective This study investigated the hepatic mechanisms of action of C. lanceolatum flowers ethanol extract (ClEtOH) related to its antihyperglycaemic effect in streptozotocin-diabetic rats. Materials and methods Male Wistar rats were divided into normal (N) and diabetic control (DC) rats treated with vehicle (water); diabetic rats treated with 500 mg/kg metformin (DMet) or 500 mg/kg ClEtOH (DT500)...
September 2016: Pharmaceutical Biology
https://www.readbyqxmd.com/read/26424783/phylogeography-and-modes-of-reproduction-in-diploid-and-tetraploid-halophytes-of-limonium-species-plumbaginaceae-evidence-for-a-pattern-of-geographical-parthenogenesis
#12
Ana Sofia Róis, Flávio Sádio, Octávio S Paulo, Generosa Teixeira, Ana Paula Paes, Dalila Espírito-Santo, Timothy F Sharbel, Ana D Caperta
BACKGROUND AND AIMS: The genus Limonium (Plumbaginaceae) has long been recognized to have sexual and apomictic (asexual seed formation) modes of reproduction. This study aimed to elucidate phylogeographical patterns and modes of reproduction in diploid and tetraploid Limonium species, namely three putative sexual diploid species with morphological affinities (L. nydeggeri, L. ovalifolium, L. lanceolatum) and three related, probably apomict tetraploid species (L. binervosum, L. dodartii, L...
January 2016: Annals of Botany
https://www.readbyqxmd.com/read/26094865/gene-regulation-in-amphioxus-an-insight-from-transgenic-studies-in-amphioxus-and-vertebrates
#13
REVIEW
Iryna Kozmikova, Zbynek Kozmik
Cephalochordates, commonly known as amphioxus or lancelets, are the most basal subphylum of chordates. Cephalochordates are thus key to understanding the origin of vertebrates and molecular mechanisms underlying vertebrate evolution. The evolution of developmental control mechanisms during invertebrate-to-vertebrate transition involved not only gene duplication events, but also specific changes in spatial and temporal expression of many genes. To get insight into the spatiotemporal regulation of gene expression during invertebrate-to-vertebrate transition, functional studies of amphioxus gene regulatory elements are highly warranted...
December 2015: Marine Genomics
https://www.readbyqxmd.com/read/25970656/tetranorsesquiterpenoids-and-santalane-type-sesquiterpenoids-from-illicium-lanceolatum-and-their-antimicrobial-activity-against-the-oral-pathogen-porphyromonas-gingivalis
#14
Miwa Kubo, Yuri Nishikawa, Kenichi Harada, Masataka Oda, Jian-Mei Huang, Hisanori Domon, Yutaka Terao, Yoshiyasu Fukuyama
The methanol extract of the leaves of Illicium lanceolatum, indigenous to Fujian Province, People's Republic of China, was found to exhibit antimicrobial activity against the periodontal pathogen Porphyromonas gingivalis, and a bioassay-guided fractionation led to the isolation of two new compounds, 1 and 2, along with two known santalane-type sesquiterpenoids, 3 and 4. The structures of lanceolactone A (1) and lanceolactone B (2) were elucidated by analyzing their 2D NMR spectroscopic data. Compounds 1 and 2 were assigned as new tetranorsesquiterpenoids with a spiroacetal ring and tricyclic structure, respectively...
June 26, 2015: Journal of Natural Products
https://www.readbyqxmd.com/read/25923116/comparing-acute-effects-of-a-nano-tio2-pigment-on-cosmopolitan-freshwater-phototrophic-microbes-using-high-throughput-screening
#15
Chu Thi Thanh Binh, Christopher G Peterson, Tiezheng Tong, Kimberly A Gray, Jean-François Gaillard, John J Kelly
Production of titanium-dioxide nanomaterials (nano-TiO2) is increasing, leading to potential risks associated with unintended release of these materials into aquatic ecosystems. We investigated the acute effects of nano-TiO2 on metabolic activity and viability of algae and cyanobacteria using high-throughput screening. The responses of three diatoms (Surirella angusta, Cocconeis placentula, Achnanthidium lanceolatum), one green alga (Scenedesmus quadricauda), and three cyanobacteria (Microcystis aeruginosa, Gloeocapsa sp...
2015: PloS One
https://www.readbyqxmd.com/read/25898604/-estimation-for-vegetation-carbon-storage-in-tiantong-national-forest-park
#16
Chun-Zi Guo, Yang-Yang Wu, Jian Ni
Based on the field investigation and the data combination from literature, vegetation carbon storage, carbon density, and their spatial distribution were examined across six forest community types (Schima superba--Castanopsis fargesii community, S. superba--C. fargesii with C. sclerophylla community, S. superba--C. fargesii with Distylium myricoides community, Illicium lanceolatum--Choerospondias axillaris community, Liquidambar formosana--Pinus massoniana community and Hedyotis auricularia--Phylostachys pubescens community) in Tiantong National Forest Park, Zhejiang Province, by using the allometric biomass models for trees and shrubs...
November 2014: Ying Yong Sheng Tai Xue Bao, the Journal of Applied Ecology
https://www.readbyqxmd.com/read/25846052/neuronal-organization-of-the-brain-in-the-adult-amphioxus-branchiostoma-lanceolatum-a-study-with-acetylated-tubulin-immunohistochemistry
#17
Antonio Castro, Manuela Becerra, María Jesús Manso, Ramón Anadón
Amphioxus (Cephalochordata) belongs to the most basal extant chordates, and knowledge of their brain organization appears to be key to deciphering the early stages of evolution of vertebrate brains. Most comprehensive studies of the organization of the central nervous system of adult amphioxus have investigated the spinal cord. Some brain populations have been characterized via neurochemistry and electron microscopy, and the overall cytoarchitecture of the brain was studied by Ekhart et al. (2003; J. Comp. Neurol...
October 15, 2015: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/25774519/identification-evolution-and-expression-of-an-insulin-like-peptide-in-the-cephalochordate-branchiostoma-lanceolatum
#18
Claire Lecroisey, Yann Le Pétillon, Hector Escriva, Eckhard Lammert, Vincent Laudet
Insulin is one of the most studied proteins since it is central to the regulation of carbohydrate and fat metabolism in vertebrates and its expression and release are disturbed in diabetes, the most frequent human metabolic disease worldwide. However, the evolution of the function of the insulin protein family is still unclear. In this study, we present a phylogenetic and developmental analysis of the Insulin Like Peptide (ILP) in the cephalochordate amphioxus. We identified an ILP in the European amphioxus Branchiostoma lanceolatum that displays structural characteristics of both vertebrate insulin and Insulin-like Growth Factors (IGFs)...
2015: PloS One
https://www.readbyqxmd.com/read/25650764/expression-of-fluorescent-proteins-in-branchiostoma-lanceolatum-by-mrna-injection-into-unfertilized-oocytes
#19
Estelle Hirsinger, João Emanuel Carvalho, Christine Chevalier, Georges Lutfalla, Jean-François Nicolas, Nadine Peyriéras, Michael Schubert
We report here a robust and efficient protocol for the expression of fluorescent proteins after mRNA injection into unfertilized oocytes of the cephalochordate amphioxus, Branchiostoma lanceolatum. We use constructs for membrane and nuclear targeted mCherry and eGFP that have been modified to accommodate amphioxus codon usage and Kozak consensus sequences. We describe the type of injection needles to be used, the immobilization protocol for the unfertilized oocytes, and the overall injection set-up. This technique generates fluorescently labeled embryos, in which the dynamics of cell behaviors during early development can be analyzed using the latest in vivo imaging strategies...
January 12, 2015: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/25262393/selection-of-wild-macrophytes-for-use-in-constructed-wetlands-for-phytoremediation-of-contaminant-mixtures
#20
Anna Guittonny-Philippe, Marie-Eléonore Petit, Véronique Masotti, Yogan Monnier, Laure Malleret, Bruno Coulomb, Isabelle Combroux, Teddy Baumberger, Julien Viglione, Isabelle Laffont-Schwob
Constructed wetlands (CWs) offer an alternative to traditional industrial wastewater treatment systems that has been proved to be efficient, cost-effective and environmentally friendly. Most of the time, CWs are planted with proliferative species such as Phragmites australis or with plants originating from nurseries, both representing a risk for the natural biodiversity conservation of aquatic ecosystems located downstream of the CWs. For the removal of metals and organic pollutant mixtures present in industrial effluents, it is necessary to select tolerant plant species that are able to produce a high aboveground biomass and to develop a healthy belowground system...
January 1, 2015: Journal of Environmental Management
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