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Anh D Nguyen, Sergei I Golovatch
The genus Enghoffosoma is recorded from Vietnam for the first time, altogether with six species involved. Two species, E. lanceolatum Likhitrakarn et al., 2014 and E. anchoriforme Likhitrakarn et al., 2014, were previously known only from the western part of Laos, thus being new to the fauna of Vietnam. One more species, Sundanina fedorenkoi Golovatch, 2016, very recently described from Dak Lak Province, southern Vietnam, is formally transferred to Enghoffosoma, comb. nov., based both on a strict topotype and a sample from Lam Dong Province, southern Vietnam...
2016: Zootaxa
Donald A Beasley, William E Walton
The developmental time and survival to eclosion of Anopheles hermsi Barr & Guptavanij fed monotypic and mixed diets of ten food types were examined in laboratory studies. Larvae fed monotypic diets containing animal detritus (freeze-dried rotifers, freeze-dried Daphnia pulicaria, and TetraMin® fish food flakes) and the mixotrophic protistan Cryptomonas ovata developed faster and survived better than larvae that were fed other monotypic diets. Survival to adulthood of larvae fed several concentrations of the diatom Planothidium (=Achnanthes) lanceolatum was poor (<13%) and larval development time was approximately twice that of larvae fed TetraMin® fish food flakes, the standard laboratory diet...
June 2016: Journal of Vector Ecology: Journal of the Society for Vector Ecology
Vladimir Ječmenica, Vincent Droissart, Nausicaa Noret, Tariq Stévart
Recent field inventories and taxonomic research in Central Africa have resulted in the discovery of many new orchid species. Five specimens of an apparently new Angraecum species were collected in Gabon and Cameroon. They stand out for their hanging habit and short zig-zag stem. Morphology of leaves and habit is somewhat comparable to Angraecum cultriforme and Angraecum stolzii, two species from East Africa. Flowers of the novelty share the general morphology of Angraecum pyriforme from which the new species is distinguished by being smaller and with a different lip-spur ratio...
2016: PhytoKeys
Serge A T Fobofou, Katrin Franke, Andrea Porzel, Wolfgang Brandt, Ludger A Wessjohann
The chemical investigation of the chloroform extract of Hypericum lanceolatum guided by (1)H NMR, ESIMS, and TLC profiles led to the isolation of 11 new tricyclic acylphloroglucinol derivatives, named selancins A-I (1-9) and hyperselancins A and B (10 and 11), along with the known compound 3-O-geranylemodin (12), which is described for a Hypericum species for the first time. Compounds 8 and 9 are the first examples of natural products with a 6-acyl-2,2-dimethylchroman-4-one core fused with a dimethylpyran unit...
April 22, 2016: Journal of Natural Products
Juliany Torres Siqueira, Emanuele Batistela, Mayara Peron Pereira, Virginia Claudia da Silva, Paulo Teixeira de Sousa Junior, Claudia Marlise Balbinotti Andrade, Nair Honda Kawashita, Gisele Lopes Bertolini, Amanda Martins Baviera
Context Ethnopharmacological studies have demonstrated that plants of the Combretum genus presented antidiabetic activity, including Combretum lanceolatum Pohl ex Eichler (Combretaceae). Objective This study investigated the hepatic mechanisms of action of C. lanceolatum flowers ethanol extract (ClEtOH) related to its antihyperglycaemic effect in streptozotocin-diabetic rats. Materials and methods Male Wistar rats were divided into normal (N) and diabetic control (DC) rats treated with vehicle (water); diabetic rats treated with 500 mg/kg metformin (DMet) or 500 mg/kg ClEtOH (DT500)...
September 2016: Pharmaceutical Biology
Ana Sofia Róis, Flávio Sádio, Octávio S Paulo, Generosa Teixeira, Ana Paula Paes, Dalila Espírito-Santo, Timothy F Sharbel, Ana D Caperta
BACKGROUND AND AIMS: The genus Limonium (Plumbaginaceae) has long been recognized to have sexual and apomictic (asexual seed formation) modes of reproduction. This study aimed to elucidate phylogeographical patterns and modes of reproduction in diploid and tetraploid Limonium species, namely three putative sexual diploid species with morphological affinities (L. nydeggeri, L. ovalifolium, L. lanceolatum) and three related, probably apomict tetraploid species (L. binervosum, L. dodartii, L...
January 2016: Annals of Botany
Iryna Kozmikova, Zbynek Kozmik
Cephalochordates, commonly known as amphioxus or lancelets, are the most basal subphylum of chordates. Cephalochordates are thus key to understanding the origin of vertebrates and molecular mechanisms underlying vertebrate evolution. The evolution of developmental control mechanisms during invertebrate-to-vertebrate transition involved not only gene duplication events, but also specific changes in spatial and temporal expression of many genes. To get insight into the spatiotemporal regulation of gene expression during invertebrate-to-vertebrate transition, functional studies of amphioxus gene regulatory elements are highly warranted...
December 2015: Marine Genomics
Miwa Kubo, Yuri Nishikawa, Kenichi Harada, Masataka Oda, Jian-Mei Huang, Hisanori Domon, Yutaka Terao, Yoshiyasu Fukuyama
The methanol extract of the leaves of Illicium lanceolatum, indigenous to Fujian Province, People's Republic of China, was found to exhibit antimicrobial activity against the periodontal pathogen Porphyromonas gingivalis, and a bioassay-guided fractionation led to the isolation of two new compounds, 1 and 2, along with two known santalane-type sesquiterpenoids, 3 and 4. The structures of lanceolactone A (1) and lanceolactone B (2) were elucidated by analyzing their 2D NMR spectroscopic data. Compounds 1 and 2 were assigned as new tetranorsesquiterpenoids with a spiroacetal ring and tricyclic structure, respectively...
June 26, 2015: Journal of Natural Products
Chu Thi Thanh Binh, Christopher G Peterson, Tiezheng Tong, Kimberly A Gray, Jean-François Gaillard, John J Kelly
Production of titanium-dioxide nanomaterials (nano-TiO2) is increasing, leading to potential risks associated with unintended release of these materials into aquatic ecosystems. We investigated the acute effects of nano-TiO2 on metabolic activity and viability of algae and cyanobacteria using high-throughput screening. The responses of three diatoms (Surirella angusta, Cocconeis placentula, Achnanthidium lanceolatum), one green alga (Scenedesmus quadricauda), and three cyanobacteria (Microcystis aeruginosa, Gloeocapsa sp...
2015: PloS One
Chun-Zi Guo, Yang-Yang Wu, Jian Ni
Based on the field investigation and the data combination from literature, vegetation carbon storage, carbon density, and their spatial distribution were examined across six forest community types (Schima superba--Castanopsis fargesii community, S. superba--C. fargesii with C. sclerophylla community, S. superba--C. fargesii with Distylium myricoides community, Illicium lanceolatum--Choerospondias axillaris community, Liquidambar formosana--Pinus massoniana community and Hedyotis auricularia--Phylostachys pubescens community) in Tiantong National Forest Park, Zhejiang Province, by using the allometric biomass models for trees and shrubs...
November 2014: Ying Yong Sheng Tai Xue Bao, the Journal of Applied Ecology
Antonio Castro, Manuela Becerra, María Jesús Manso, Ramón Anadón
Amphioxus (Cephalochordata) belongs to the most basal extant chordates, and knowledge of their brain organization appears to be key to deciphering the early stages of evolution of vertebrate brains. Most comprehensive studies of the organization of the central nervous system of adult amphioxus have investigated the spinal cord. Some brain populations have been characterized via neurochemistry and electron microscopy, and the overall cytoarchitecture of the brain was studied by Ekhart et al. (2003; J. Comp. Neurol...
October 15, 2015: Journal of Comparative Neurology
Claire Lecroisey, Yann Le Pétillon, Hector Escriva, Eckhard Lammert, Vincent Laudet
Insulin is one of the most studied proteins since it is central to the regulation of carbohydrate and fat metabolism in vertebrates and its expression and release are disturbed in diabetes, the most frequent human metabolic disease worldwide. However, the evolution of the function of the insulin protein family is still unclear. In this study, we present a phylogenetic and developmental analysis of the Insulin Like Peptide (ILP) in the cephalochordate amphioxus. We identified an ILP in the European amphioxus Branchiostoma lanceolatum that displays structural characteristics of both vertebrate insulin and Insulin-like Growth Factors (IGFs)...
2015: PloS One
Estelle Hirsinger, João Emanuel Carvalho, Christine Chevalier, Georges Lutfalla, Jean-François Nicolas, Nadine Peyriéras, Michael Schubert
We report here a robust and efficient protocol for the expression of fluorescent proteins after mRNA injection into unfertilized oocytes of the cephalochordate amphioxus, Branchiostoma lanceolatum. We use constructs for membrane and nuclear targeted mCherry and eGFP that have been modified to accommodate amphioxus codon usage and Kozak consensus sequences. We describe the type of injection needles to be used, the immobilization protocol for the unfertilized oocytes, and the overall injection set-up. This technique generates fluorescently labeled embryos, in which the dynamics of cell behaviors during early development can be analyzed using the latest in vivo imaging strategies...
2015: Journal of Visualized Experiments: JoVE
Anna Guittonny-Philippe, Marie-Eléonore Petit, Véronique Masotti, Yogan Monnier, Laure Malleret, Bruno Coulomb, Isabelle Combroux, Teddy Baumberger, Julien Viglione, Isabelle Laffont-Schwob
Constructed wetlands (CWs) offer an alternative to traditional industrial wastewater treatment systems that has been proved to be efficient, cost-effective and environmentally friendly. Most of the time, CWs are planted with proliferative species such as Phragmites australis or with plants originating from nurseries, both representing a risk for the natural biodiversity conservation of aquatic ecosystems located downstream of the CWs. For the removal of metals and organic pollutant mixtures present in industrial effluents, it is necessary to select tolerant plant species that are able to produce a high aboveground biomass and to develop a healthy belowground system...
January 1, 2015: Journal of Environmental Management
S Moisset, S Kim Tiam, A Feurtet-Mazel, S Morin, F Delmas, N Mazzella, P Gonzalez
This study examined the effects of diuron on strains of three major freshwater diatom species, Eolimna minima, Nitzschia palea and Planothidium lanceolatum. These species are frequently recorded in the Morcille River, where diuron runs off during phytosanitary treatments of the vineyards around. Here, there were three diatom exposure groups for each species: 0, 1 and 10 μg/L diuron during a 14-day laboratory assessment. Diuron water concentration, cell number, photosynthetic activity and gene expression were assessed at 6 h and 2, 7 and 14 days after contamination...
March 2015: Environmental Science and Pollution Research International
Gheorghe Dărăbuş, Mihăită Afrenie, Ionela Hotea, Mirela Imre, Sorin Morariu
Animals from seven zoological gardens located in Romania, including 18 species of herbivores, 10 species of carnivores, and 13 species of omnivores, were screened for the presence of parasites. Overall, the prevalences of parasites identified in the sampled population were 54.2% (58/107) for herbivores, 54.5% (24/44) for carnivores, and 32.6% (17/52) for omnivores. In herbivores, Eimeria spp., Dicrocelium lanceolatum, and pulmonary and digestive strongyles were detected. In carnivores, the genera Eimeria and Cystoisospora and nematodes from Ancylostomatidae, Strongyloidae, Ascaridae, Capillariidae and Trichocephalidae were identified...
June 2014: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
Natdanai Likhitrakarn, Sergei I Golovatch, Somsak Panha
The Southeast Asian millipede genus Enghoffosoma ranges from southern China in the north, western Myanmar in the west, and central Thailand and southern Laos in the south and east. It currently contains seven species, including five new ones, described in this paper: E. zebra sp. n., E. lanceolatum sp. n., E. anchoriforme sp. n., E. funda sp. n. and E. bispinum sp. n. The genus is rediagnosed, a key to all known species is given, and their distributions are mapped.
2014: Zootaxa
Yong Wang, Minghua Mei, Yongnian Ni, Serge Kokot
A novel combined near- and mid-infrared (NIR and MIR) spectroscopic method has been researched and developed for the analysis of complex substances such as the Traditional Chinese Medicine (TCM), Illicium verum Hook. F. (IVHF), and its noxious adulterant, Iuicium lanceolatum A.C. Smith (ILACS). Three types of spectral matrix were submitted for classification with the use of the linear discriminant analysis (LDA) method. The data were pretreated with either the successive projections algorithm (SPA) or the discrete wavelet transform (DWT) method...
September 15, 2014: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Ming Zhao, Xianming Zhang, Yan Wang, Min Huang, Jin-Ao Duan, Tanja Gödecke, Karina M Szymulanska-Ramamurthy, Zhiqi Yin, Chun-Tao Che
Three new germacrane sesquiterpenes and a new m-menthane monoterpene were isolated together with four known compounds from the pericarp of Illicium lanceolatum, an adulterant to star anise (Illicium verum). All compounds were isolated from Illicium plants for the first time. The absolute stereochemistry of all germacranes and m-menthane was established by a combination of NMR and the modified Mosher's ester method. The biological activity was evaluated on SH-SY5Y neuroblastoma cell line. (1S,5R,7R)-1,5-Dihydroxygermacra-4(15),10(14),11(12)-triene (at 62...
2014: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Barnabas Bessem Orang-Ojong, Jose Edward Munyangaju, Ma Shang Wei, Miao Lin, Fan Guan Wei, Charles Foukunang, Yan Zhu
Cancer is a significant public health concern and treatment poses a problem and is frequently unsuccessful. As such, continuous efforts in the search for new agents and therapies to improve survival are required. A considerable number of plant extracts and isolated compounds possess significant anti-proliferative or pro-apoptotic effects. The majority of biologically active compounds belong to terpenoids, phenolics and alkaloids. Terpenoid plants such as Hypericum lanceolatum and a few alkaloid plants have been found to be potent anti-parasitic agents but have exhibited poor antimicrobial effects...
July 2013: Molecular and Clinical Oncology
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