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Homeodomain

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https://www.readbyqxmd.com/read/28107201/homeodomain-interacting-protein-kinase-2-hipk2-a-promising-target-for-anti-cancer-therapies
#1
Yuanyuan Feng, Lihong Zhou, Xiaoting Sun, Qi Li
The HIPK2 (serine/threonine homeodomain-interacting protein kinase 2) is a "caretaker" gene, its inactivation increases tumorigenicity while its activation inhibits tumor growth. This report reviews the anti-tumorigenic mechanisms of HIPK2, which include promotion of apoptosis, inhibition of angiogenesis in hypoxia, prevention of tumor invasion/metastasis and attenuation of multidrug resistance in cancer. Additionally, we summarize conditions or factors that may increase HIPK2 activity.
January 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28101231/the-biological-significance-of-methylome-differences-in-human-papilloma-virus-associated-head-and-neck-cancer
#2
Maria J Worsham, Kang Mei Chen, Indrani Datta, Josena K Stephen, Dhananjay Chitale, Alexandra Gothard, George Divine
In recent years, studies have suggested that promoter methylation in human papilloma virus (HPV) positive head and neck squamous cell carcinoma (HNSCC) has a mechanistic role and has the potential to improve patient survival. The present study aimed to replicate key molecular findings from previous analyses of the methylomes of HPV positive and HPV negative HNSCC in an independent cohort, to assess the reliability of differentially methylated markers in HPV-associated tumors. HPV was measured using real-time quantitative PCR and the biological significance of methylation differences was assessed by Ingenuity Pathway Analysis (IPA)...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28100297/transcriptional-analysis-of-mating-and-pre-infection-stages-of-the-anther-smut-microbotryum-lychnidis-dioicae
#3
Su San Toh, Zehua Chen, David J Schultz, Christina A Cuomo, Michael H Perlin
Microbotryum lychnidis-dioicae is an obligate biotrophic parasite of the wildflower species, Silene latifolia. This dikaryotic fungus, commonly known as an anther smut, requires that haploid, yeast-like sporidia of opposite mating types fuse and differentiate into dikaryotic hyphae that penetrate host tissue as part of the fungal lifecycle. Mating occurs under conditions of cool temperatures and limited nutrients. Further development requires host cues or chemical mimics, including a variety of lipids, e.g...
January 17, 2017: Microbiology
https://www.readbyqxmd.com/read/28094776/new-insights-into-cooperative-binding-of-homeodomain-transcription-factors-prep1-and-pbx1-to-dna
#4
Chiara Zucchelli, Elena Ferrari, Francesco Blasi, Giovanna Musco, Chiara Bruckmann
PREP1 and PBX1 are homeodomain (HD) transcription factors that play crucial roles in embryonic development. Here, we present the first biophysical characterization of a PREP1 HD, and the NMR spectroscopic study of its DNA binding pocket. The data show that residues flanking the HD participate in DNA binding. The kinetic parameters for DNA binding of individual PREP1 and PBX1 HDs, and of their combination, show that isolated PREP1 and PBX1 HDs bind to DNA in a cooperative manner. A novel PREP1 motif, flanking the HD at the C-terminus, is required for cooperativity...
January 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28094761/homeodomain-protein-otp-affects-developmental-neuropeptide-switching-in-oxytocin-neurons-associated-with-a-long-term-effect-on-social-behavior
#5
Einav Wircer, Janna Blechman, Nataliya Borodovsky, Michael Tsoory, Ana Rita Nunes, Rui F Oliveira, Gil Levkowitz
Proper response to stress and social stimuli depends on orchestrated development of hypothalamic neuronal circuits. Here we address the effects of the developmental transcription factor orthopedia (Otp) on hypothalamic development and function. We show that developmental mutations in the zebrafish paralogous gene otpa but not otpb affect both stress response and social preference. These behavioral phenotypes were associated with developmental alterations in oxytocinergic (OXT) neurons. Thus, otpa and otpb differentially regulate neuropeptide switching in a newly identified subset of OXT neurons that co-express the corticotropin-releasing hormone (CRH)...
January 17, 2017: ELife
https://www.readbyqxmd.com/read/28092121/hox-functional-diversity-novel-insights-from-flexible-motif-folding-and-plastic-protein-interaction
#6
Miguel Ortiz-Lombardia, Nicolas Foos, Corinne Maurel-Zaffran, Andrew J Saurin, Yacine Graba
How the formidable diversity of forms emerges from developmental and evolutionary processes is one of the most fascinating questions in biology. The homeodomain-containing Hox proteins were recognized early on as major actors in diversifying animal body plans. The molecular mechanisms underlying how this transcription factor family controls a large array of context- and cell-specific biological functions is, however, still poorly understood. Clues to functional diversity have emerged from studies exploring how Hox protein activity is controlled through interactions with PBC class proteins, also evolutionary conserved HD-containing proteins...
January 16, 2017: BioEssays: News and Reviews in Molecular, Cellular and Developmental Biology
https://www.readbyqxmd.com/read/28060737/elevated-uhrf1-expression-contributes-to-poor-prognosis-by-promoting-cell-proliferation-and-metastasis-in-hepatocellular-carcinoma
#7
Xincheng Liu, Huohui Ou, Leyang Xiang, Xianghong Li, Yu Huang, Dinghua Yang
Ubiquitin-like with plant homeodomain and ring finger domains, 1 (UHRF1) is overexpressed in a variety of tumor tissues and is negatively correlated with prognosis of patients with cancers, yet so far, a comprehensive study of UHRF1 in hepatocellular carcinoma (HCC) has not been conducted. The present study was designed to explore the expression of UHRF1, associated clinical implications, and its possible functions in HCC. Reverse transcription-polymerase chain reaction and immunohistochemical staining were used to detect UHRF1 expression in HCC specimens including cancerous and noncancerous tissues...
January 2, 2017: Oncotarget
https://www.readbyqxmd.com/read/28055972/integrative-genomic-and-transcriptomic-analysis-for-pinpointing-recurrent-alterations-of-plant-homeodomain-genes-and-their-clinical-significance-in-breast-cancer
#8
Huimei Yu, Yuanyuan Jiang, Lanxin Liu, Wenqi Shan, Xiaofang Chu, Zhe Yang, Zeng-Quan Yang
A wide range of the epigenetic effectors that regulate chromatin modification, gene expression, genomic stability, and DNA repair contain structurally conserved domains called plant homeodomain (PHD) fingers. Alternations of several PHD finger-containing proteins (PHFs) due to genomic amplification, mutations, deletions, and translocations have been linked directly to various types of cancer. However, little is known about the genomic landscape and the clinical significance of PHFs in breast cancer. Hence, we performed a large-scale genomic and transcriptomic analysis of 98 PHF genes in breast cancer using TCGA and METABRIC datasets and correlated the recurrent alterations with clinicopathological features and survival of patients...
December 31, 2016: Oncotarget
https://www.readbyqxmd.com/read/28049756/a-role-for-prolyl-isomerase-pin1-in-the-phosphorylation-dependent-modulation-of-prrxl1-function
#9
Ricardo Soares-Dos-Reis, Ana Sofia Pessoa, Ana Filipa Dias, Miguel Falcão, Mariana Raimundo Matos, Rui Vitorino, Filipe Almeida Monteiro, Deolinda Lima, Carlos Reguenga
Prrxl1 encodes for a paired-like homeodomain transcription factor essential for the correct establishment of the dorsal root ganglion - spinal cord nociceptive circuitry during development. Prrxl1 -null mice display gross anatomical disruption of this circuitry, which translates to a markedly diminished sensitivity to noxious stimuli. Here, by the use of an Immunoprecipitation-Mass Spectrometry approach, we identify 5 highly conserved phosphorylation sites (T110, S119, S231, S233, S251) in PRRXL1 primary structure...
January 3, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28036027/the-phosphorylation-of-pdx-1-by-protein-kinase-ck2-is-crucial-for-its-stability
#10
Sabrina Klein, Rui Meng, Mathias Montenarh, Claudia Götz
The homeodomain protein PDX-1 is a critical regulator of pancreatic development and insulin production in pancreatic β-cells. We have recently shown that PDX-1 is a substrate of protein kinase CK2; a multifunctional protein kinase which is implicated in the regulation of various cellular aspects, such as differentiation, proliferation, and survival. The CK2 phosphorylation site of PDX-1 is located within the binding region of the E3 ubiquitin ligase adaptor protein PCIF1. To study the interaction between PDX-1 and PCIF1 we used immunofluorescence analysis, co-immunoprecipitation, GST-pull-down studies, and proximity ligation assay (PLA)...
December 28, 2016: Pharmaceuticals
https://www.readbyqxmd.com/read/28028241/abscisic-acid-signaling-is-controlled-by-a-branched1-hd-zip-i-cascade-in-arabidopsis-axillary-buds
#11
Eduardo González-Grandío, Alice Pajoro, José M Franco-Zorrilla, Carlos Tarancón, Richard G H Immink, Pilar Cubas
Shoot-branching patterns determine key aspects of plant life and are important targets for crop breeding. However, we are still largely ignorant of the genetic networks controlling locally the most important decision during branch development: whether the axillary bud, or branch primordium, grows out to give a lateral shoot or remains dormant. Here we show that, inside the buds, the TEOSINTE BRANCHED1, CYCLOIDEA, PCF (TCP) transcription factor BRANCHED1 (BRC1) binds to and positively regulates the transcription of three related Homeodomain leucine zipper protein (HD-ZIP)-encoding genes: HOMEOBOX PROTEIN 21 (HB21), HOMEOBOX PROTEIN 40 (HB40), and HOMEOBOX PROTEIN 53 (HB53)...
December 27, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28018056/onecut-transcription-factors-in-development-and-disease
#12
Peter A Kropp, Maureen Gannon
Developmental processes are remarkably well conserved among species, and among the most highly conserved developmental regulators are transcription factor families. The Onecut transcription factor family consists of three members known for their single "cut" DNA-binding domain and an aberrant homeodomain. The three members of the Onecut family are highly conserved from Drosophila to humans and have significant roles in regulating the development of diverse tissues derived from the ectoderm or endoderm, where they activate a number of gene families...
2016: Trends in Developmental Biology
https://www.readbyqxmd.com/read/28008082/whole-transcriptome-response-to-water-stress-in-a-california-endemic-oak-quercus-lobata
#13
Paul F Gugger, Juan Manuel Peñaloza-Ramírez, Jessica W Wright, Victoria L Sork
Reduced water availability during drought can create major stress for many plant species. Within a species, populations with a history of seasonal drought may have evolved the ability to tolerate drought more than those in areas of high precipitation and low seasonality. In this study, we assessed response to water stress in a California oak species, Quercus lobata Née, by measuring changes in gene expression profiles before and after a simulated drought stress treatment through water deprivation of seedlings in a greenhouse setting...
December 21, 2016: Tree Physiology
https://www.readbyqxmd.com/read/28005979/cloning-and-functional-analysis-of-pax6-from-the-hydrothermal-vent-tubeworm-ridgeia-piscesae
#14
Huifang Yuan, Wei Wang, Bin Hu, Changkun Pan, Mingliang Chen, Linlin Ke, Lirong Yang, Jianming Chen
The paired box 6 (Pax6) gene encodes a transcription factor essential for eye development in a wide range of animal lineages. Here we describe the cloning and characterization of Pax6 gene from the blind hydrothermal vent tubeworm Ridgeia piscesae (RpPax6). The deduced RpPax6 protein shares extensive sequence identity with Pax6 proteins from other species and contains both the paired domain and a complete homeodomain. Phylogenetic analysis indicates that it clusters with the corresponding sequence from the closely related species Platynereis dumerilii (P...
2016: PloS One
https://www.readbyqxmd.com/read/28004105/uhrf2-decreases-h3k9ac-expression-by-interacting-with-it-through-the-phd-and-sra-ydg-domain-in-hepg2-hepatocellular-carcinoma-cells
#15
Ting Zhang, Linglin Zhao, Shengyuan Zeng, Lu Bai, Junxia Chen, Zheng Zhang, Yalan Wang, Changzhu Duan
Ubiquitin-like with PHD and ring finger domains 2 (UHRF2) is a multi-domain E3 ubiquitin ligase which is involved in epigenetic regulation and plays an essential role in tumorigenesis. However, the role of UHRF2 in histone H3 acetylation has not yet been fully elucidated and few studies have reported its role in hepatocellular carcinoma (HCC). In this study, we examined the correlation between UHRF2 and acetylated H3 in HCC. Immunohistochemistry and western blot analysis demonstrated that the levels of histone H3 lysine 9 acetylation (H3K9ac) and histone H3 lysine 14 acetylation (H3K14ac) were higher in the HCC tissues and HepG2 HCC cells compared with the adjacent non-tumor tissues and L02 normal cells...
January 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28002617/ago4-is-specifically-required-for-heterochromatic-sirna-accumulation-at-pol-v-dependent-loci-in-arabidopsis-thaliana
#16
Feng Wang, Michael J Axtell
In plants, 24 nucleotide long heterochromatic siRNAs (het-siRNAs) transcriptionally regulate gene expression by RNA-directed DNA methylation (RdDM). The biogenesis of most het-siRNAs depends on the plant-specific RNA polymerase IV (Pol IV), and ARGONAUTE4 (AGO4) is a major het-siRNA effector protein. Through genome-wide analysis of sRNA-seq data sets, we found that AGO4 is required for the accumulation of a small subset of het-siRNAs. The accumulation of AGO4-dependent het-siRNAs also requires several factors known to participate in the effector portion of the RdDM pathway, including RNA POLYMERASE V (POL V), DOMAINS REARRANGED METHYLTRANSFERASE 2 (DRM2) and SAWADEE HOMEODOMAIN HOMOLOG 1 (SHH1)...
December 21, 2016: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28000708/protein-inhibitor-of-activated-stat-y-piasy-regulates-insulin-secretion-by-interacting-with-lim-homeodomain-transcription-factor-isl1
#17
Chengzhi Yan, Chulin Yu, Di Zhang, Yan Cui, Jinlian Zhou, Sheng Cui
It is known that the LIM homeodomain transcription factor Isl1 is highly expressed in all pancreatic endocrine cells and functions in regulating pancreatic development and insulin secretion. The Isl1 mutation has been found to be associated with type 2 diabetes, but the mechanism responsible for Isl1 regulation of insulin synthesis and secretion still needs to be elucidated. In the present study, the protein inhibitor of activated STAT Y (PIASy) was identified as a novel Isl1-interacting protein with a yeast two-hybrid system, and its interaction with Isl1 was further confirmed by a co-immunoprecipitation experiment...
December 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27986639/identification-of-the-binding-domains-and-key-amino-acids-for-the-interaction-of-the-transcription-factors-bmpoum2-and-bmabd-a-in-bombyx-mori
#18
Yong He, Huimin Deng, Qihao Hu, Zidan Zhu, Lin Liu, Sichun Zheng, Qisheng Song, Qili Feng
The transcription factor BmPOUM2 interacted with another transcription factor BmAbd-A to regulate the expression of the wing cuticle protein gene BmWCP4 in Bombyx mori. In this study, the binding domains and amino acids for the interaction between BmPOUM2 and BmAbd-A were reported. Two isoforms of BmPOUM2 were identified. The short isoform (BmPOUM2-S) lacks a 114-amino acid sequence containing a POU-homeodomain and a nuclear localization signal peptide (NLS), as compared to the full-length isoform (BmPOUM2)...
December 14, 2016: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27956701/the-chromatin-associated-phf12-protein-maintains-nucleolar-integrity-and-prevents-premature-cellular-senescence
#19
Richard Graveline, Katarzyna Marcinkiewicz, Seyun Choi, Marilène Paquet, Wolfgang Wurst, Thomas Floss, Gregory David
PF1, also known as PHF12 (plant homeodomain (PHD) zinc finger protein 12) is a member of the PHD zinc finger family of proteins. PF1 associates with a chromatin interacting protein complex comprised of MRG15, Sin3B, and HDAC1, that functions as a transcriptional modulator. The biological function of Pf1 remains largely elusive. We undertook the generation of Pf1 knockout mice to elucidate its physiological role. We demonstrate that Pf1 is required for mid-to-late gestation viability. Pf1 inactivation impairs the proliferative potential of the mouse embryonic fibroblasts (MEFs), and is associated with a significant decrease in BrdU incorporation, an increase in SA-β-gal activity, a marker of cellular senescence and elevated levels of phosphorylated H2AX (γ-H2AX), a marker associated with DNA double-strand breaks...
December 12, 2016: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27951410/non-syndromic-severe-hypodontia-caused-by-a-novel-frameshift-insertion-mutation-in-the-homeobox-of-the-msx1-gene
#20
Mushriq F Abid, M A Simpson, Christos Petridis, M T Cobourne, P T Sharpe
OBJECTIVE: Inherited congenital anomalies in tooth number, particularly hypodontia are relatively common. Although substantial progress has been made that permits a better understanding of the causes of tooth agenesis, overall knowledge of the phenotype:genotype correlations in this anomaly are still lacking. The aim in this study was to identify the causal gene mutation(s) in a family of two sisters with severe hypodontia (oligodontia) including 2nd premolars and 1st and 3rd molars, using whole exome sequencing (WES)...
December 6, 2016: Archives of Oral Biology
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