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https://www.readbyqxmd.com/read/29104110/pseudomyogenic-hemangioendothelioma-of-skin-bone-and-soft-tissue-a-clinicopathological-immunohistochemical-and-fluorescence-in-situ-hybridization-study
#1
Dinesh Pradhan, Karen Schoedel, Richard L McGough, Sarangarajan Ranganathan, Uma N M Rao
Pseudomyogenic hemangioendothelioma (PHE) is an uncommon neoplasm with propensity for local recurrence. The tumor mimics epithelioid hemangioendothelioma and epithelioid sarcoma, representing a possible diagnostic pitfall. We investigated the clinicopathological, immunohistochemical and fluorescence in situ hybridization features of PHEs. Eight cases of PHE were retrieved from our pathology archives. The clinical and outcome information was available in six patients. In six cases the tumors were located in the lower limb while the upper limb was involved in two cases...
November 2, 2017: Human Pathology
https://www.readbyqxmd.com/read/29072561/enterococcus-wangshanyuanii-sp-nov-isolated-from-faeces-of-yaks-bos-grunniens
#2
Dong Jin, Jing Yang, Shan Lu, Xin-He Lai, YanWen Xiong, Jianguo Xu
The taxonomic position of four phenotypically closely related strains isolated from faecal samples of yaks (Bos grunniens) collected from the Qinghai-Tibetan plateau, China, was determined by a polyphasic approach. The strains were non-spore-forming, non-motile Gram-stain-positive, ovoid cocci, occurring predominantly in pairs and short chains or in irregular clusters. The 16S rRNA gene of strain MN05(T) was related phylogenetically to those of Enterococcushaemoperoxidus, Enterococcusrotai, Enterococcusquebecensis, Enterococcusplantarum, Enterococcuscrotali, Enterococcusmoraviensis, Enterococcussilesiacus, Enterococcuscaccae, Enterococcustermitis, Enterococcusureasiticus and Enterococcusureilyticus, all belonging to the Enterococcusfaecalis species group...
October 26, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29058648/lactobacillus-musae-sp-nov-a-novel-lactic-acid-bacterium-isolated-from-banana-fruits
#3
Yi-Sheng Chen, Li-Ting Wang, Yu-Jou Liao, Yi-Shan Lan, Chi-Huan Chang, Yu-Chung Chang, Hui-Chung Wu, Huei-Yin Lo, Misa Otoguro, Fujitoshi Yanagida
Two Gram-stain-positive, catalase-negative, rod-shaped, bacterial strains (313(T) and 311) were isolated from banana fruits in Taiwan. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the highest similarity to both strains corresponded to the type strain of Lactobacillus nantensis (99.19 %), followed by Lactobacillus crustorum (98.99 %), Lactobacillus heilongjiangensis (98.59 %) and Lactobacillus farciminis (98.52 %). Phylogenetic analysis based on the sequences of two housekeeping genes, pheS and rpoA, revealed that these two strains were well separated from the Lactobacillus reference strains...
October 23, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29034853/floricoccus-tropicus-gen-nov-sp-nov-and-floricoccus-penangensis-sp-nov-isolated-from-fresh-flowers-of-durian-tree-and-hibiscus
#4
Li-Oon Chuah, Kien-Pong Yap, Ahamed Kamal Shamila-Syuhada, Kwai Lin Thong, Rosma Ahmad, Min Tze Liong, Gulam Rusul
Three strains of Gram-staining-positive, coccus-shaped, lactic acid bacteria, designated as HibF3(T), HibF2 and HibF5 were isolated from fresh flowers of hibiscus, and a fourth, DF1(T), was isolated from fresh flowers of durian tree, in Penang, Malaysia. Taxonomic characterisation was performed by polyphasic analysis. Sequence similarities of the 16S rRNA gene and the housekeeping rpoA and pheS genes of these strains with their closely-related lactococcal and streptococcal relatives were 92-94, 78 and 81 %, respectively...
October 16, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28971067/the-role-of-the-regulator-fur-in-gene-regulation-and-virulence-of-riemerella-anatipestifer-assessed-using-an-unmarked-gene-deletion-system
#5
Yunqing Guo, Di Hu, Jie Guo, Xiaowen Li, Jinyue Guo, Xiliang Wang, Yuncai Xiao, Hui Jin, Mei Liu, Zili Li, Dingren Bi, Zutao Zhou
Riemerella anatipestifer, an avian pathogen, has resulted in enormous economic losses to the duck industry globally. Notwithstanding, little is known regarding the physiological, pathogenic and virulence mechanisms of Riemerella anatipestifer (RA) infection. However, the role of Ferric uptake regulator (Fur) in the virulence of R. anatipestifer has not, to date, been demonstrated. Using a genetic approach, unmarked gene deletion system, we evaluated the function of fur gene in the virulence of R. anatipestifer...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28919805/a-pilot-evaluation-of-a-computer-based-psychometric-test-battery-designed-to-detect-impairment-in-patients-with-cirrhosis
#6
Nicola A Cook, Jin Un Kim, Yasmin Pasha, Mary Me Crossey, Adrian J Schembri, Brian T Harel, Torben Kimhofer, Simon D Taylor-Robinson
BACKGROUND: Psychometric testing is used to identify patients with cirrhosis who have developed hepatic encephalopathy (HE). Most batteries consist of a series of paper-and-pencil tests, which are cumbersome for most clinicians. A modern, easy-to-use, computer-based battery would be a helpful clinical tool, given that in its minimal form, HE has an impact on both patients' quality of life and the ability to drive and operate machinery (with societal consequences). AIM: We compared the Cogstate™ computer battery testing with the Psychometric Hepatic Encephalopathy Score (PHES) tests, with a view to simplify the diagnosis...
2017: International Journal of General Medicine
https://www.readbyqxmd.com/read/28895518/lactobacillus-curtus-sp-nov-isolated-from-beer-in-finland
#7
Yuki Asakawa, Nobuchika Takesue, Shizuka Asano, Satoshi Shimotsu, Kazumaru Iijima, Koji Suzuki, Yasuo Motoyama, Masayuki Aizawa
A Gram-stain-positive, catalase-negative and short-rod-shaped organism, designated VTT E-94560, was isolated from beer in Finland and deposited in the VTT culture collection as a strain of Lactobacillus rossiae. However, the results of 16S rRNA gene sequence analysis showed that VTT E-94560 was only related to Lactobacillus rossiae JCM 16176(T) with 97.0 % sequence similarity, lower than the 98.7 % regarded as the boundary for the species differentiation. Additional phylogenetic studies on the pheS gene, rpoA gene and 16S-23S rRNA internally transcribed spacer region further reinforced the taxonomically independent status of VTT E-94560 and its related Lactobacillus species including L...
October 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28867002/lactobacillus-cerevisiae-sp-nov-isolated-from-a-spoiled-brewery-sample
#8
Jennifer Koob, Fritz Jacob, Mareike Wenning, Mathias Hutzler
A Gram-stain-positive, non-motile, rod-shaped bacterium, designated TUM BP 140423000-2250T (=DSM 100836T=LMG 29073T), was isolated from spoiled beer. This bacterium did not form spores, and was catalase-negative and facultatively anaerobic. Its taxonomic position was determined in a polyphasic study. The 16S rRNA gene sequence similarity data showed that the strain belonged to the Lactobacillus genus with the nearest neighbours being Lactobacillus koreensis DCY50T (sequence similarity 99.5 %), Lactobacillus yonginensis THK-V8T (99...
September 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820096/lactobacillus-caviae-sp-nov-an-obligately-heterofermentative-bacterium-isolated-from-the-oral-cavity-of-a-guinea-pig-cavia-aperea-f-porcellus
#9
Jiri Killer, Radko Pechar, Pavel Švec, Hana Salmonová, Roman Švejstil, Martina Geigerová, Vojtěch Rada, Eva Vlková, Chahrazed Mekadim
A Gram-stain-positive, facultatively anaerobic, and catalase- and oxidase-negative bacterial strain designated MOZM2T, having 98.4 % 16S rRNA gene sequence identity with Lactobacillus reuteri DSM 20016T, was isolated from a swab of the oral cavity of a home-bred guinea pig. Comparative analyses based on the hsp60, pheS and tuf genes confirmed L. reuteri as its closest relative species, with calculated sequence similarities of 92.8, 88.8 and 96.9 %, respectively. DNA-DNA hybridisation revealed a 42 % degree of genetic similarity between the novel strain and L...
August 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28738305/contamination-characteristics-and-source-apportionment-of-methylated-pahs-in-agricultural-soils-from-yangtze-river-delta-china
#10
Weixiao Chen, Xinyi Wu, Haiyun Zhang, Jianteng Sun, Wenxin Liu, Lizhong Zhu, Xiangdong Li, Daniel C W Tsang, Shu Tao, Xilong Wang
Alkylated PAHs (APAHs) have been shown to be more toxic and persistent than their non-alkylated parent compounds. However, little is known about the extent of soil contamination by these pollutants. To help understand agricultural soil pollution by these compounds at a regional scale, a total of 18 methylated PAHs (MPAHs, a major class of APAHs) in 243 soil samples were analyzed. These soil samples were collected from 11 sites in the Yangtze River Delta (YRD) region, a representative fast developing area in China...
November 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28679374/development-of-a-counterselectable-seamless-mutagenesis-system-in-lactic-acid-bacteria
#11
Yongping Xin, Tingting Guo, Yingli Mu, Jian Kong
BACKGROUND: Lactic acid bacteria (LAB) are receiving more attention to act as cell factories for the production of high-value metabolites. However, the molecular tools for genetic modifying these strains are mainly vector-based double-crossover strategies, which are laborious and inefficient. To address this problem, several counterselectable markers have been developed, while few of them could be used in the wild-type host cells without pretreatment. RESULTS: The pheS gene encoding phenylalanyl-tRNA synthetase alpha subunit was identified in Lactococcus lactis NZ9000 genome...
July 5, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28667481/disrupted-topological-organization-of-brain-structural-network-associated-with-prior-overt-hepatic-encephalopathy-in-cirrhotic-patients
#12
Hua-Jun Chen, Hai-Bin Shi, Long-Feng Jiang, Lan Li, Rong Chen
OBJECTIVES: To investigate structural brain connectome alterations in cirrhotic patients with prior overt hepatic encephalopathy (OHE). METHODS: Seventeen cirrhotic patients with prior OHE (prior-OHE), 18 cirrhotic patients without prior OHE (non-prior-OHE) and 18 healthy controls (HC) underwent diffusion tensor imaging. Neurocognitive functioning was assessed with Psychometric Hepatic Encephalopathy Score (PHES). Using a probabilistic fibre tracking approach, we depicted the whole-brain structural network as a connectivity matrix of 90 regions (derived from the Automated Anatomic Labeling atlas)...
June 30, 2017: European Radiology
https://www.readbyqxmd.com/read/28632114/enterococcus-crotali-sp-nov-isolated-from-faecal-material-of-a-timber-rattlesnake
#13
Richard W McLaughlin, Patricia Lynn Shewmaker, Anne M Whitney, Ben W Humrighouse, Ana C Lauer, Vladimir N Loparev, Christopher A Gulvik, P A Cochran, Scot E Dowd
A facultatively anaerobic, Gram-stain-positive bacterium, designated ETRF1T, was found in faecal material of a timber rattlesnake (Crotalus horridus). Based on a comparative 16S rRNA gene sequence analysis, the isolate was assigned to the genus Enterococcus. The 16S rRNA gene sequence of strain ETRF1T showed >97 % similarity to that of the type strains of Enterococcus rotai, E. caccae, E. silesiacus, E haemoperoxidus, E. ureasiticus, E. moraviensis, E. plantarum, E. quebecensis, E. ureilyticus, E. termitis, E...
June 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28617525/characterization-of-high-exopolysaccharide-producing-lactobacillus-strains-isolated-from-mustard-pickles-for-potential-probiotic-applications
#14
Jing-Yao Huang, Cheng-Yen Kao, We-Sin Liu, Tony J Fang
The aim of this study was to characterize high exopolysaccharide (EPS)-producing lactic acid bacteria (LAB) isolated from mustard pickles in Taiwan for potential probiotic applications. Among 39 collected LAB strains, four most productive EPS-producing strains were selected for further analysis. Comparative analyses of 16S rDNA genes rpoA and pheS sequences demonstrated that these strains were members of Lactobacillus plantarum-group (LPG). NCD 2, NLD 4, SLC 13, and NLD 16 showed survival rates of 95.83% ± 0...
June 2017: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28580550/magnetic-signature-geochemistry-and-oral-bioaccessibility-of-technogenic-metals-in-contaminated-industrial-soils-from-sindos-industrial-area-northern-greece
#15
Anna Bourliva, Lambrini Papadopoulou, Elina Aidona, Katerina Giouri
The objective of this study was to assess the contamination level of potentially harmful elements (PHEs) in industrial soils and how this relates to environmental magnetism. Moreover, emphasis was given to the determination of the potential mobile fractions of typically "technogenic" metals. Therefore, magnetic and geochemical parameters were determined in topsoils (0-20 cm) collected around a chemical industry in Sindos Industrial Area, Thessaloniki, Greece. Soil samples were presented significantly enriched in "technogenic" metals such Cd, Pb, and Zn, while cases of severe soil contamination were observed in sampling sites north-west of the industrial unit...
July 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28573602/cognitive-reserve-is-a-resilience-factor-for-cognitive-dysfunction-in-hepatic-encephalopathy
#16
Piero Amodio, Sara Montagnese, Giuseppe Spinelli, Sami Schiff, Daniela Mapelli
Cognitive Reserve (CR) modulates symptoms of brain disease. The aim of this study was: to evaluate the effect of CR on cognition in cirrhosis and on the mismatch between cognitive and neurophysiologic assessment of hepatic encephalopathy (HE). Eighty-two outpatient patients with cirrhosis without overt HE were studied [73% males; age: 62 (54-68) (median, interq. range) yrs.; education: 8 (6-13) yrs.]. The Psychometric Hepatic Encephalopathy Score (PHES) was used as cognitive measure of HE. The spectral analysis of the electroencephalogram (EEG) was used as neurophysiologic measure of HE...
August 2017: Metabolic Brain Disease
https://www.readbyqxmd.com/read/28557520/periodic-health-examinations-and-missed-opportunities-among-patients-likely-needing-mental-health-care
#17
Ming Tai-Seale, Laura A Hatfield, Caroline J Wilson, Cheryl D Stults, Thomas G McGuire, Lisa C Diamond, Richard M Frankel, Lisa MacLean, Ashley Stone, Jennifer Elston Lafata
OBJECTIVES: Periodic health examinations (PHEs) are the most common reason adults see primary care providers. It is unknown if PHEs serve as a "safe portal" for patients with mental health needs to initiate care. We examined how physician communication styles impact mental health service delivery in PHEs. STUDY DESIGN: Retrospective observational study using audio-recordings of 255 PHEs with patients likely to need mental health care. METHODS: Mixed-methods examined the timing of a mental health discussion (MHD), its quality, and the relationship between MHD quality and physician practice styles...
October 1, 2016: American Journal of Managed Care
https://www.readbyqxmd.com/read/28499087/identification-of-intrahelical-bifurcated-h-bonds-as-a-new-type-of-gate-in-k-channels
#18
Oliver Rauh, Martin Urban, Leonhard M Henkes, Tobias Winterstein, Timo Greiner, James L Van Etten, Anna Moroni, Stefan M Kast, Gerhard Thiel, Indra Schroeder
Gating of ion channels is based on structural transitions between open and closed states. To uncover the chemical basis of individual gates, we performed a comparative experimental and computational analysis between two K(+) channels, KcvS and KcvNTS. These small viral encoded K(+) channel proteins, with a monomer size of only 82 amino acids, resemble the pore module of all complex K(+) channels in terms of structure and function. Even though both proteins share about 90% amino acid sequence identity, they exhibit different open probabilities with ca...
June 7, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28406121/normalization-of-the-psychometric-hepatic-encephalopathy-score-for-diagnosis-of-minimal-hepatic-encephalopathy-in-turkey
#19
Bdo Coskun, M Ozen, S Gursoy, O Ozbakir, O K Poyrazoglu, M Baskol, G C Sezgin, M Yucesoy
AIM: To construct normal values for the tests of the psychometric hepatic encephalopathy score (PHES) and evaluate the prevalence of minimal hepatic encephalopathy (MHE) among Turkish patients with liver cirrhosis. MATERIALS AND METHODS: One hundred and eighty-five healthy subjects and sixty patients with liver cirrhosis without overt hepatic encephalopathy were included in the study. All subjects underwent psychometric tests, which include number connection test-A and B (NCT-A/B), serial dotting test (DST), line drawing test (LDT), and digit symbol test (DST) in the same day...
April 2017: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/28316320/critical-flicker-frequency-test-for-diagnosing-minimal-hepatic-encephalopathy-in-patients-with-cirrhosis
#20
Banu Demet Özel Coşkun, Mustafa Özen
BACKGROUND/AIMS: The critical flicker frequency (CFF) and psychometric hepatic encephalopathy score (PHES) are commonly proposed tests for detecting minimal hepatic encephalopathy (MHE); however, no studies have examined their value for detecting MHE in Turkey. MATERIALS AND METHODS: A total of 70 patients with cirrhosis without overt HE, 205 controls for PHES, and 100 controls for the CFF test were included. All the patients underwent the PHES and CFF tests during the same session...
May 2017: Turkish Journal of Gastroenterology: the Official Journal of Turkish Society of Gastroenterology
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