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https://www.readbyqxmd.com/read/28719356/co-incubation-of-pmn-and-caco-2-cells-modulates-inflammatory-potential
#1
M B Schaefer, C A Schaefer, M Hecker, R E Morty, M Witzenrath, W Seeger, K Mayer
Polymorphonuclear granulocytes (PMN) are activated in inflammatory reactions. Intestinal epithelial cells are relevant for maintaining the intestinal barrier. We examined interactions of PMN and intestinal epithelial cell-like CaCo-2 cells to elucidate their regulation of inflammatory signalling and the impact of cyclooxygenase (COX), nitric oxide (NO) and platelet-activating factor (PAF). Human PMN and CaCo-2 cells, separately and in co-incubation, were stimulated with the calcium ionophore A23187 or with N-Formyl-methionyl-leucyl-phenylalanin (fMLP) that activates PMN only...
May 20, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28698201/entinostat-neutralizes-myeloid-derived-suppressor-cells-and-enhances-the-antitumor-effect-of-pd-1-inhibition-in-murine-models-of-lung-and-renal-cell-carcinoma
#2
Ashley Orillion, Ayumi Hashimoto, Nur P Damayanti, Li Shen, Remi Adelaiye-Ogala, Sreevani Arisa, Sreenivasulu Chintala, Peter Ordentlich, Chinghai Kao, Bennett Elzey, Dmitry Gabrilovich, Roberto Pili
Purpose: Recent advances in immunotherapy highlight the antitumor effects of immune- checkpoint inhibition despite a relatively limited subset of patients receiving clinical benefit. The selective class I histone deacetylase inhibitor (HDACi) entinostat has been reported to have immunomodulatory activity including targeting of immune suppressor cells in the tumor microenvironment. Thus, we decided to assess whether entinostat could enhance anti-PD-1 treatment and investigate those alterations in the immunosuppressive tumor microenvironment that contribute to the combined anti-tumor activity...
July 11, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28690058/flow-cytometric-assessment-of-myeloperoxidase-in-bovine-blood-neutrophils-and-monocytes
#3
Elke Depreester, Evelyne Meyer, Kristel Demeyere, Mieke Van Eetvelde, Miel Hostens, Geert Opsomer
Myeloperoxidase (MPO) is a lysosomal peroxidase enzyme mainly stored in the azurophilic granules of neutrophils playing an important role in innate immunity for first-line protection against microorganisms in many species including cattle. As such, determination of MPO has become of great interest for the diagnosis of infectious and inflammatory diseases in multiple species such as humans. In cattle, MPO determination is rarely done because methods to assess MPO in this species are limited: functional assays have been described earlier, but so far, the quantification of MPO at the single cell level has not been done yet...
July 6, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28687659/neutrophils-induce-astroglial-differentiation-and-migration-of-human-neural-stem-cells-via-c1q-and-c3a-synthesis
#4
Mitra J Hooshmand, Hal X Nguyen, Katja M Piltti, Francisca Benavente, Samuel Hong, Lisa Flanagan, Nobuko Uchida, Brian J Cummings, Aileen J Anderson
Inflammatory processes play a key role in pathophysiology of many neurologic diseases/trauma, but the effect of immune cells and factors on neurotransplantation strategies remains unclear. We hypothesized that cellular and humoral components of innate immunity alter fate and migration of human neural stem cells (hNSC). In these experiments, conditioned media collected from polymorphonuclear leukocytes (PMN) selectively increased hNSC astrogliogenesis and promoted cell migration in vitro. PMN were shown to generate C1q and C3a; exposure of hNSC to PMN-synthesized concentrations of these complement proteins promoted astrogliogenesis and cell migration...
July 7, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28682250/effects-of-bi-zn2-3nb1-3-o3-modification-on-the-relaxor-behavior-and-piezoelectricity-of-pb-mg1-3nb2-3-o3-pbtio3-ceramics
#5
Zenghui Liu, Hua Wu, Alisa Paterson, Wei Ren, Zuo-Guang Ye
Relaxor lead magnesium niobate (PMN)-based materials exhibit complex structures and unusual properties that have been puzzling researchers for decades. In this work, a new ternary solid solution of Pb(Mg₁/₃Nb₂/₃)O₃-PbTiO₃- Bi(Zn₂/₃Nb₁/₃)O₃ (PMN-PT-BZN) is prepared in the form of ceramics, and the effects of the incorporation of BZN into the PMN-PT binary system are investigated. The crystal structure favors a pseudocubic symmetry and the relaxor properties are enhanced as the concentration of BZN increases...
June 29, 2017: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
https://www.readbyqxmd.com/read/28680095/osteopontin-facilitates-west-nile-virus-neuroinvasion-via-neutrophil-trojan-horse-transport
#6
Amber M Paul, Dhiraj Acharya, Laurel Duty, E Ashley Thompson, Linda Le, Dobrivoje S Stokic, A Arturo Leis, Fengwei Bai
West Nile virus (WNV) can cause severe human neurological diseases including encephalitis and meningitis. The mechanisms by which WNV enters the central nervous system (CNS) and host-factors that are involved in WNV neuroinvasion are not completely understood. The proinflammatory chemokine osteopontin (OPN) is induced in multiple neuroinflammatory diseases and is responsible for leukocyte recruitment to sites of its expression. In this study, we found that WNV infection induced OPN expression in both human and mouse cells...
July 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28674178/ccl5-promotes-resolution-phase-macrophage-reprogramming-in-concert-with-the-atypical-chemokine-receptor-d6-and-apoptotic-polymorphonuclear-cells
#7
Miran Aswad, Simaan Assi, Sagie Schif-Zuck, Amiram Ariel
The engulfment of apoptotic polymorphonuclear cells (PMN) during the resolution of inflammation leads to macrophage reprogramming culminating in reduced proinflammatory and increased anti-inflammatory mediator secretion. The atypical chemokine receptor D6/ACKR2 is expressed on apoptotic PMN and plays an important role in regulating macrophage properties during and after engulfment. In this study, we found that the inflammatory chemokine CCL5 is mostly retained (75%) during the resolution of zymosan A peritonitis in mice...
July 3, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28669381/role-of-nets-in-the-difference-in-host-susceptibility-to-toxoplasma-gondii-between-sheep-and-cattle
#8
Kader Yildiz, Sami Gokpinar, Aycan Nuriye Gazyagci, Cahit Babur, Neslihan Sursal, Ahmet Kursat Azkur
The main aim of this study was to compare extracellular traps (NETs) formation by polymorphonuclear neutrophils (PMNs) of cattle and sheep when exposed to T. gondii tachyzoites in vitro. The effects of parasite concentrations and different incubation periods on NETs development in cattle and sheep PMNs were studied. The effect of NET structures on host cell invasion by tachyzoites was also studied. This is the first report of NETs development by sheep and cattle PMNs against T. gondii in vitro. T. gondii-induced extracellular DNA production from PMNs was dependent on tachyzoite concentrations and incubation time in both sheep and cattle...
July 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28663087/superiority-of-aromatase-inhibitor-and-cyclooxygenase-2-inhibitor-combined-delivery-hyaluronate-targeted-versus-pegylated-protamine-nanocapsules-for-breast-cancer-therapy
#9
Ahmed O Elzoghby, Shaimaa K Mostafa, Maged W Helmy, Maha A ElDemellawy, Salah A Sheweita
Despite several reports have revealed the beneficial effect of co-administration of COX-2 inhibitors with aromatase inhibitors in managing postmenopausal breast cancer; no nanocarriers for such combined delivery have been developed till now. Therefore, protamine nanocapsules (PMN-NCs) have been developed to co-deliver letrozole (LTZ) that inhibits aromatase-mediated estrogen biosynthesis and celecoxib (CXB) that synergistically inhibits aromatase expression. Inspired by the CD44-mediated tumor targeting ability of hyaluronate (HA), we developed HA-coated PMN-NCs (HA-NCs) via electrostatic layer-by-layer assembly...
June 27, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28657526/useful-of-tokyo-guidelines-in-the-diagnosis-of-acute-cholecystitis-anatomopathologie-correlationship
#10
Christian Janikow, Edgardo Corti, Nicolás Menso, Gastón Moretti, Martín Alé, Martín Sánchez, Paul Eduardo Lada
BACKGROUND: In the year 2007 a group of experts come together to discuss criteria for acute cholecystitis and to establish therapeutic guidelines and states of gravity in this disease. OBJECTIVES: we correlated the criteria of the Tokyo Guidelines 2007 with the anatomopathology study of the surgical specimen. SETTING: Service of Urgencies of the National Clinic Hospital in Córdoba, Argentine. METHODS: We studied 324 patients (120 male and 204 female) older than 15 years and without limits of age with the criteria of acute cholecystitis a cord to the Tokyo guidelines 2007...
2017: Revista de la Facultad de Ciencias Médicas
https://www.readbyqxmd.com/read/28655995/microrna-142-3p-and-let-7g-negatively-regulates-augmented-il-6-production-in-neonatal-polymorphonuclear-leukocytes
#11
Hsin-Chun Huang, Hong-Ren Yu, Te-Yao Hsu, I-Lun Chen, Hui-Chen Huang, Jen-Chieh Chang, Kuender D Yang
Neonatal PMN are qualitatively impaired in functions, yet they frequently reveal augmented inflammatory reactions during sepsis. Here, we hypothesized that PMN from newborns produce more IL-6 than those from adults under LPS stimulation, in which transcriptional or posttranscriptional regulation is involved in the altered expression. We found that neonatal PMN produced significantly higher IL-6 mRNA and protein than adult PMN. The higher IL-6 expression was not related to transcriptional but posttranscriptional regulation as the IL-6 expression was affected by the addition of cycloheximide but not actinomycin...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28655817/antibody-against-microbial-neuraminidases-recognizes-human-sialidase-3-neu3-the-neuraminidase-sialidase-superfamily-revisited
#12
Chiguang Feng, Jihong Li, Greg Snyder, Wei Huang, Simeon E Goldblum, Wilbur H Chen, Lai-Xi Wang, Bruce A McClane, Alan S Cross
Neuraminidases (NAs) are critical virulence factors for several microbial pathogens. With a highly conserved catalytic domain, a microbial NA "superfamily" has been proposed. We previously reported that murine polymorphonuclear leukocyte (PMN) sialidase activity was important in leukocyte trafficking to inflamed sites and that antibodies to Clostridium perfringens NA recognized a cell surface molecule(s), presumed to be a sialidase of eukaryotic origin on interleukin-8-stimulated human and murine PMNs. These antibodies also inhibited cell sialidase activity both in vitro and, in the latter instance, in vivo We therefore hypothesized that mammalian sialidases share structural homology and epitopes with microbial NAs...
June 27, 2017: MBio
https://www.readbyqxmd.com/read/28654190/interobserver-variability-in-scoring-liver-biopsies-with-a-diagnosis-of-alcoholic-hepatitis
#13
Bela Horvath, Daniela Allende, Hao Xie, John Guirguis, Jennifer Jeung, James Lapinski, Deepa Patil, Arthur J McCullough, Srinivasan Dasarathy, Xiuli Liu
BACKGROUND: Alcoholic hepatitis (AH) is one of the most severe forms of alcoholic liver disease. Recently, a histologic scoring system for predicting prognosis in this patient cohort was proposed as Alcoholic Hepatitis Histologic Score (AHHS). We aimed to assess interobserver variability in recognizing histologic features of AH, and the effect of this variability on the proposed AHHS categories. DESIGN: Hematoxylin-eosin and trichrome stained slides from 32 patients diagnosed with AH with liver biopsies within 1 month of presentation (2000-2015), were reviewed by 5 pathologists including 3 liver pathologists and 2 gastrointestinal (GI) pathologists masked to the clinical findings or outcome...
June 27, 2017: Alcoholism, Clinical and Experimental Research
https://www.readbyqxmd.com/read/28650621/novel-cdta-based-bifunctional-chelators-for-stable-and-inert-mn-ii-complexation-synthesis-and-physicochemical-characterization
#14
Christian Vanasschen, Enikő Molnár, Gyula Tircsó, Ferenc K Kálmán, Éva Tóth, Marie Brandt, Heinz H Coenen, Bernd Neumaier
In the search for Mn(II) MR and PET/MR imaging agents with optimal balance between thermodynamic stability, kinetic inertness, and relaxivity, two novel bifunctional Mn(II) chelators (BFMnCs) based on CDTA (trans-1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid) were synthesized. A six-step synthesis, involving the buildup of a functionalized trans-1,2-diaminocyclohexane core, provided CuAAC-reactive 6a and 6b bearing an alkyne or azide substituent on the cyclohexane ring, respectively (CuAAC = Cu(I)-catalyzed azide-alkyne 1,3-dipolar cycloaddition)...
July 17, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28638921/-protective-effect-of-synthetic-salidroside-on-acute-lung-injury-in-rats
#15
Qian Huang, Yan-Chun Cai, Xiao-Li Wei, Jin-Long Wu, Ru-Huan Mei, Xiao-Lan Hu
To study the protective effect and mechanism of synthetic salidroside on acute lung injury (ALI) induced by lipopolysaccharide (LPS), male Sprague-Dawley (SD) rats were randomly divided into saline control group, 3 mg/kg LPS model group, different doses of salidroside groups (5, 20 and 80 mg/kg), and 5 mg/kg dexamethasone group. Intratracheal LPS instillation was used to establish the ALI model 0.5 h after intraperitoneal injection of salidroside or dexamethasone, and the rats were sacrificed 6 h later. Lung wet/dry weight ratio (W/D) was calculated...
June 25, 2017: Sheng Li Xue Bao: [Acta Physiologica Sinica]
https://www.readbyqxmd.com/read/28627430/changes-in-eosinophil-count-during-bacterial-infection-revisiting-an-old-marker-to-assess-the-efficacy-of-antimicrobial-therapy
#16
B Davido, S Makhloufi, M Matt, R Calin, O Senard, C Perronne, A Dinh, J Salomon
INTRODUCTION: Eosinopenia as a criterion of sepsis has been the subject of debate for decades. Different authors have proposed different cut-off values. METHODS: A prospective study was conducted from February to August 2016. Hospitalized adults suffering from a bacterial infection with eosinopenia, defined as an eosinophil count <100/mm(3), were included. Patients were divided into two groups according to the first day of effective antimicrobial therapy. They were observed for 5days in order to evaluate whether recovery from eosinopenia was predictive of an appropriate antibiotic regimen...
June 13, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28615221/coronin-1a-a-novel-player-in-integrin-biology-controls-neutrophil-trafficking-in-innate-immunity
#17
Robert Pick, Daniela Begandt, Thomas J Stocker, Melanie Salvermoser, Sarah Thome, Ralph T Böttcher, Eloi Montanez, Ute Harrison, Ignasi Forné, Alexander G Khandoga, Raffaele Coletti, Ludwig T Weckbach, Doris Brechtefeld, Rainer Haas, Axel Imhof, Steffen Massberg, Markus Sperandio, Barbara Walzog
Trafficking of polymorphonuclear neutrophils (PMNs) during inflammation critically depends on the β2 integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). Here, we identify Coronin 1A (Coro1A) as a novel regulator of β2 integrins that interacts with the cytoplasmic tail of CD18 and is crucial for induction of PMN adhesion and post-adhesion events, including adhesion strengthening, spreading, and migration under flow conditions. Transition of PMN rolling to firm adhesion critically depends on Coro1A by regulating the accumulation of high affinity LFA-1 in focal zones of adherent cells...
June 14, 2017: Blood
https://www.readbyqxmd.com/read/28614271/serum-anti-pla2r-antibody-and-glomerular-pla2r-deposition-in-chinese-patients-with-membranous-nephropathy-a-cross-sectional-study
#18
COMPARATIVE STUDY
Lu Pang, Ai-Min Zhang, Hai-Xia Li, Jia-Lin Du, Li-Li Jiao, Nan Duan, Yi Liu, Dan Yu
M-type phospholipase A2 receptor (PLA2R) is the major target antigen in primary membranous nephropathy (PMN). Previous studies have evaluated the diagnostic value of serum anti-PLA2R antibody. However, the correlation of serum anti-PLA2R antibody and glomerular PLA2R deposition, and their association with clinical characteristics need to be further evaluated.A total of 136 patients were involved as inception group because serum anti-PLA2R antibody and glomerular PLA2R antigen were simultaneously measured. We examined serum anti-PLA2R antibody by ELISA and glomerular PLA2R deposition by immunofluorescence assay...
June 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28611772/bovine-polymorphonuclear-neutrophils-cast-neutrophil-extracellular-traps-against-the-abortive-parasite-neospora-caninum
#19
Rodolfo Villagra-Blanco, Liliana M R Silva, Tamara Muñoz-Caro, Zhengtao Yang, Jianhua Li, Ulrich Gärtner, Anja Taubert, Xichen Zhang, Carlos Hermosilla
Neospora caninum represents a relevant apicomplexan parasite causing severe reproductive disorders in cattle worldwide. Neutrophil extracellular trap (NET) generation was recently described as an efficient defense mechanism of polymorphonuclear neutrophils (PMN) acting against different parasites. In vitro interactions of bovine PMN with N. caninum were analyzed at different ratios and time spans. Extracellular DNA staining was used to illustrate the typical molecules of NETs [i.e., histones (H3), neutrophil elastase (NE), myeloperoxidase (MPO), pentraxin] via antibody-based immunofluorescence analyses...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28608441/transfusion-related-acute-lung-injury-critical-neutrophil-activation-by-anti-hla-a2-antibodies-for-endothelial-permeability
#20
Kathy Khoy, Minh Vu Chuong Nguyen, Dominique Masson, Béatrice Bardy, Christian Drouet, Marie-Hélène Paclet
BACKGROUND: Transfusion-related acute lung injury (TRALI) is a major complication of hemotherapy that may occur after the transfusion of any blood type component. Several clinical reports have suggested the presence of anti-HLA antibodies in the blood product. This study sought to examine the role of anti-HLA-A2 antibodies in polymorphonuclear neutrophil (PMN) activation and thus in endothelial permeability. STUDY DESIGN AND METHODS: PMN activation was assessed by both nicotinamide adenine dinucleotide phosphate oxidase (NADPH oxidase) activity and reactive oxygen species (ROS) production...
June 13, 2017: Transfusion
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