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biomarkers for i fertility

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https://www.readbyqxmd.com/read/27932553/normalization-matters-tracking-the-best-strategy-for-sperm-mirna-quantification
#1
Celia Corral-Vazquez, Joan Blanco, Albert Salas-Huetos, Francesca Vidal, Ester Anton
STUDY QUESTION: What is the most reliable normalization strategy for sperm microRNA (miRNA) quantitative Reverse Transcription Polymerase Chain Reactions (qRT-PCR) using singleplex assays? SUMMARY ANSWER: The use of the average expression of hsa-miR-100-5p and hsa-miR-30a-5p as sperm miRNA qRT-PCR data normalizer is suggested as an optimal strategy. WHAT IS KNOWN ALREADY: Mean-centering methods are the most reliable normalization strategies for miRNA high-throughput expression analyses...
December 8, 2016: Molecular Human Reproduction
https://www.readbyqxmd.com/read/27823786/non-invasive-metabolomics-for-improved-determination-of-embryonic-sex-markers-in-chemically-defined-culture-medium
#2
E Gómez, M Muñoz, C Simó, C Ibáñez, S Carrocera, D Martín-González, A Cifuentes
Metabolic differences between early male and female embryos can be reflected in culture medium (CM). We used a single bovine embryo culture step (24h) supporting improved birth rates under chemically defined conditions (CDC) to investigate biomarker detection of embryonic sex in contrast to classical BSA-containing medium. In vitro matured slaughterhouse oocytes were fertilized in vitro with a single bull. Embryos were initially cultured in synthetic oviduct fluid with BSA. On day-6, morulae were cultured individually in droplets with (BSA) or without protein (CDC)...
November 1, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27785402/embryo-quality-predictive-models-based-on-cumulus-cells-gene-expression
#3
R Devjak, T Burnik Papler, I Verdenik, K Fon Tacer, E Vrtačnik Bokal
Since the introduction of in vitro fertilization (IVF) in clinical practice of infertility treatment, the indicators for high quality embryos were investigated. Cumulus cells (CC) have a specific gene expression profile according to the developmental potential of the oocyte they are surrounding, and therefore, specific gene expression could be used as a biomarker. The aim of our study was to combine more than one biomarker to observe improvement in prediction value of embryo development. In this study, 58 CC samples from 17 IVF patients were analyzed...
July 1, 2016: Balkan Journal of Medical Genetics: BJMG
https://www.readbyqxmd.com/read/27743772/development-of-a-fluorescent-protein-antibody-f%C3%A3-rster-resonance-energy-transfer-probe-for-the-detection-and-imaging-of-osteocalcin
#4
Chan-I Chung, Ryoji Makino, Yuki Ohmuro-Matsuyama, Hiroshi Ueda
Fluorescence-based biosensor probes, especially those based on Förster resonance energy transfer (FRET) between fluorescent protein (FP) variants, are widely used to monitor various biological phenomena, often detecting ligand-induced association of the receptor domains. While antibodies are fertile sources of specific receptors for various biomolecules, their potential has not been fully exploited. In this study, we used a fluorescent probe comprising FP-fused antibody variable region fragments to detect a bone metabolism biomarker, osteocalcin (BGP), by using fluorescence spectrometry/microscopy...
October 12, 2016: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/27559149/differences-in-cumulus-cell-gene-expression-indicate-the-benefit-of-a-pre-maturation-step-to-improvein-vitro-bovine-embryo-production
#5
Cecilia Dieci, Valentina Lodde, Rémi Labreque, Isabelle Dufort, Irene Tessaro, Marc-André Sirard, Alberto M Luciano
STUDY QUESTION: Does the gene expression profile of cumulus cells (CC) accompanying oocytes with different degrees of chromatin compaction within the germinal vesicle (GV) reflect the oocyte's quality and response in culture during in-vitro embryo production (IVP). SUMMARY ANSWER: The transcriptomic profile of the CC is related to oocyte competence, setting the stage for the development of customized pre-maturation strategies to improve IVP. WHAT IS KNOWN ALREADY: Oocytes complete the acquisition of their competence during antral follicle development...
August 24, 2016: Molecular Human Reproduction
https://www.readbyqxmd.com/read/27485360/panel-of-autoimmune-markers-for-noninvasive-diagnosis-of-minimal-mild-endometriosis-a-multicenter-study
#6
Rahul Gajbhiye, Trupti Bendigeri, Arun Ghuge, Kashmira Bhusane, Shahina Begum, Neeta Warty, Raj Sawant, Kedar Padte, Anil Humane, Pramathes Dasmahapatra, Anahita Chauhan, Shagufta Khan
Endometriosis, characterized by the presence of endometrial-like tissue at extrauterine sites, is a common, chronic, estrogen-dependent, inflammatory condition associated with pelvic pain, subfertility, dysmenorrhea, and dyspareunia, affecting about 10% of reproductive-age women in any population. The diagnosis of endometriosis is usually delayed on an average by 8 to 11 years leading to significant consequences in terms of disease progression. The current study was aimed to validate enzyme-linked immunosorbent assay based on the epitopes of stomatin-like protein 2, tropomodulin 3 (TMOD3), and tropomyosin 3 (TPM3) for diagnosis of minimal-mild endometriosis (revised American Fertility Society Classification (rAFS) stage I-II) and to compare the performance with the reported markers: cancer antigen (CA) 125, CA19-9, α-enolase, Serine/threonine-protein kinase (PDIK1L), and syntaxin 5...
July 8, 2016: Reproductive Sciences
https://www.readbyqxmd.com/read/27272931/live-cell-quantification-and-comparison-of-mammalian-oocyte-cytosolic-lipid-content-between-species-during-development-and-in-relation-to-body-composition-using-nonlinear-vibrational-microscopy
#7
Joshua Jasensky, Andrew P Boughton, Alexander Khmaladze, Jun Ding, Chi Zhang, Jason E Swain, George W Smith, Zhan Chen, Gary D Smith
Cytosolic lipids participate in the growth, development, and overall health of mammalian oocytes including many roles in cellular homeostasis. Significant emphasis has been placed on the study of lipids as a dynamic organelle, which in turn requires the development of tools and techniques to quantitate and compare how lipid content relates to cellular structure, function, and normalcy. Objectives of this study were to determine if nonlinear vibrational microscopy (e.g., coherent anti-Stokes Raman scattering or CARS microscopy) could be used for live-cell imaging to quantify and compare lipid content in mammalian oocytes during development and in relation to body composition; and compare its efficacy to methods involving cellular fixation and staining protocols...
August 7, 2016: Analyst
https://www.readbyqxmd.com/read/27209151/apolipoprotein-b-is-regulated-by-gonadotropins-and-constitutes-a-predictive-biomarker-of-ivf-outcomes
#8
Elodie Scalici, Shaliha Bechoua, Karine Astruc, Laurence Duvillard, Thomas Gautier, Véronique Drouineaud, Clément Jimenez, Samir Hamamah
BACKGROUND: Follicular fluid (FF) is an important micro-environment influencing oocyte growth, its development competence, and embryo viability. The FF content analysis allows to identify new relevant biomarkers, which could be predictive of in vitro fertilization (IVF) outcomes. Inside ovarian follicle, the amount of FF components from granulosa cells (GC) secretion, could be regulated by gonadotropins, which play a major role in follicle development. METHODS: This prospective study included 61 female undergoing IVF or Intra-cytoplasmic sperm injection (ICSI) procedure...
2016: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/27108371/testicular-damage-and-farming-environments-an-integrative-ecotoxicological-link
#9
Carolina Parelho, Filipe Bernardo, Ricardo Camarinho, Armindo Santos Rodrigues, Patrícia Garcia
The exposure to agrochemicals during farming activities affects the function of the reproductive system, as revealed by the increasing worldwide evidence of male infertility amongst farmers. The main objective of this study was to untangle the link between agricultural practices and male reproductive impairment due to chronic exposure to xenobiotics (such as agrochemicals) in conventional and organic farming environments. For this purpose, male wild mice (Mus musculus) populations from sites representing two distinct farming practices (conventional and organic farming systems) were used as bioindicators for observable effects of testicular damage, namely on a set of histological and cellular parameters: (i) relative volumetric density of different spermatogenic cells and interstitial space; (ii) damage in the seminiferous tubules and (iii) apoptotic cells in the germinal epithelium...
July 2016: Chemosphere
https://www.readbyqxmd.com/read/26893166/viability-and-dna-fragmentation-of-rainbow-trout-embryos-oncorhynchus-mykiss-obtained-from-eggs-stored-at-4%C3%A2-%C3%A2-c
#10
A Ubilla, I Valdebenito, M E Árias, J Risopatrón
In vitro storage of salmonid eggs leads to aging of the cells causing a decline in quality and reducing their capacity to develop and produce embryos. The quality of salmonid embryos is assessed by morphologic analyses; however, data on the application of biomarkers to determine the cell viability and DNA integrity of embryos in these species are limited. The aim of this study was to evaluate the effect on embryo development, viability and DNA fragmentation in the embryonic cells of in vitro storage time at 4 °C of rainbow trout (Oncorhynchus mykiss) eggs...
May 2016: Theriogenology
https://www.readbyqxmd.com/read/26861074/global-transcriptional-expression-in-ovarian-follicles-from-tsaiya-ducks-anas-platyrhynchos-with-a-high-fertilization-rate
#11
Shyh-Jong Wu, Yu-Shin Cheng, Hsiao-Lung Liu, Hsing-He Wang, Hsiu-Lin Huang
Novel candidates for biomarkers of a high-fertilization rate were identified here through global transcriptional profiling of ovarian follicles. Some other differentially expressed candidate genes were first noted to influence animal reproduction in our previous cDNA microarray analysis and are now recognized as markers for marker-assisted selection. In the present study, we compared gene expression in ovarian follicles from animals with high- and low-fertilization rates using an oligonucleotide array. On the basis of a fold change of greater than 1...
May 2016: Theriogenology
https://www.readbyqxmd.com/read/26846869/anogenital-distance-as-a-marker-of-androgen-exposure-in-humans
#12
REVIEW
A Thankamony, V Pasterski, K K Ong, C L Acerini, I A Hughes
Abnormal foetal testis development has been proposed to underlie common disorders of the male reproductive system such as cryptorchidism, hypospadias, reduced semen quality and testicular germ cell tumour, which are regarded as components of a 'testicular dysgenesis syndrome'. The increasing trends and geographical variation in their incidence have been suggested to result from in utero exposure to environmental chemicals acting as endocrine disruptors. In rodents, the anogenital distance (AGD), measured from the anus to the base of genital tubercle, is a sensitive biomarker of androgen exposure during a critical embryonic window of testis development...
July 2016: Andrology
https://www.readbyqxmd.com/read/26838846/probing-suitable-therapeutic-nanoparticles-for-controlled-drug-delivery-and-diagnostic-reproductive-health-biomarker-development
#13
Rakhi Jha, Pradeep K Jha, Santosh Gupta, S P Bhuvaneshwaran, Maidul Hossain, Sujoy K Guha
Nanomaterial mediated drug delivery represents a highly promising technique while its selectivity for reproductive healthcare application still remains a challenge. Since the delicate structure and functional role of reproductive tissue and gametes require the use of biocompatible nanomedicine/devices that do not affect fertility or the development of resulting offspring, this paper reports an intercomparative study of human spermatozoa interaction with three different nanoparticles (NPs) namely; iron oxide (Fe3O4), multiwalled carbon nanotubes (MWCNT) and graphene platelet nanopowder (GPN) to probe their suitability for drug delivery carrier and biomarker development purposes...
April 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/26758460/variability-in-follicular-fluid-high-density-lipoprotein-particle-components-measured-in-ipsilateral-follicles
#14
Keewan Kim, Michael S Bloom, Victor Y Fujimoto, Erin M Bell, Recai M Yucel, Richard W Browne
PURPOSE: The purpose of this study was to examine the biological variability of follicular fluid (FF) high density lipoprotein (HDL) particle components measured in ipsilateral ovarian follicles. METHODS: We collected FF from two ipsilateral follicles among six women undergoing in vitro fertilization (IVF). We measured concentrations of 19 FF HDL particle components, including HDL cholesterol, free cholesterol, four cholesteryl esters, phospholipids, triglycerides, paraoxonase and arylesterase activities, apolipoproteins A-1 and A-2 (ApoA-1 and ApoA-2), and seven lipophilic micronutrients, by automated analysis and with high-performance liquid chromatography...
March 2016: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/26569048/genital-cancers-in-women-ovarian-cancer
#15
Angela L Kuznia, Michelle A Roett
More than 20,000 US women are diagnosed with ovarian cancer each year. The average lifetime risk is 1.3%, but risk increases with BRCA1 or BRCA2 gene mutations (40% and 18% risk, respectively, by age 70 years) or hereditary nonpolyposis colorectal cancer syndrome (12% lifetime risk). Other risk factors include smoking, possibly past clomiphene use, and more years of ovulation. Symptoms are nonspecific. Abdominal pain is most common; others include pelvic pain, bloating, and early satiety. When ovarian cancer is suspected, evaluation should begin with transvaginal ultrasonography with Doppler studies...
November 2015: FP Essentials
https://www.readbyqxmd.com/read/26232150/predictive-biomarkers-of-preterm-delivery-in-women-with-ongoing-ivf-pregnancies
#16
Tomi T Kanninen, Giovanni Sisti, Ilana Ramer, Dan Goldschlag, Steven S Witkin, Steven D Spandorfer
In vitro fertilization (IVF) pregnancies potentially have a higher rate of preterm delivery (PTD) than do spontaneously conceived gestations, and differences persist following adjustment for multiple gestation, maternal age, and parity. The reasons for this increased susceptibility to PTD remain incompletely elucidated. To identify potential biomarkers predictive of PTD in IVF subjects, we performed a retrospective analysis of multiple markers in sera obtained during early gestation that have been suggested to be associated with peri-implantation events...
November 2015: Journal of Reproductive Immunology
https://www.readbyqxmd.com/read/26094490/preconception-folic-acid-use-influences-the-follicle-fluid-proteome
#17
John M Twigt, Karel Bezstarosti, Jeroen Demmers, Jan Lindemans, Joop S E Laven, Régine P Steegers-Theunissen
BACKGROUND: The investigation of the human follicle fluid proteome has gained much interest in the search of new markers as predictors for in vitro fertilization and intracytoplasmic sperm injection (IVF/ICSI) treatment outcome. Follicular fluid folate, as substrate of one carbon (1-C) metabolism, affects follicular metabolism and oocyte and embryo quality. From this background, we aim to identify a folate-related follicle fluid proteome that associates with IVF/ICSI treatment outcome...
August 2015: European Journal of Clinical Investigation
https://www.readbyqxmd.com/read/25959140/proteomic-characterization-of-the-qualitative-and-quantitative-differences-in-cervical-mucus-composition-during-the-menstrual-cycle
#18
G Grande, D Milardi, F Vincenzoni, G Pompa, A Biscione, A L Astorri, E Fruscella, A De Luca, I Messana, M Castagnola, R Marana
The chemical composition of the cervical mucus (CM), its physical characteristics and the volume of secretion change cyclically throughout the menstrual cycle. The aim of this study was to identify the constitutive protein composition of CM of fertile women and the changes in the CM proteome throughout the menstrual cycle. Five fertile women who had a term delivery within 1 year before the study were enrolled. Proteomic analysis was performed using an Ultimate 3000 Nano/Micro-HPLC apparatus equipped with an FLM-3000-Flow manager module and coupled with an LTQ Orbitrap XL hybrid mass spectrometer; bioinformatic software was used for functional and quantitative analysis...
June 2015: Molecular BioSystems
https://www.readbyqxmd.com/read/25904636/variation-in-maternal-urinary-cortisol-profiles-across-the-peri-conceptional-period-a-longitudinal-description-and-evaluation-of-potential-functions
#19
P A Nepomnaschy, K G Salvante, L Zeng, C Pyles, H Ma, J C Blais, L Wen, C K Barha
STUDY QUESTION: How do women's first morning urinary cortisol levels, a marker of stress axis activity, vary during the peri-conceptional period (the 12 weeks around conception)? SUMMARY ANSWER: First morning urinary cortisol follows an overall increasing trajectory across the peri-conceptional period, interrupted by 2 week-long decreases during the week preceding conception and the fifth week following conception. WHAT IS KNOWN ALREADY: Later gestational stages (i...
June 2015: Human Reproduction
https://www.readbyqxmd.com/read/25744083/oocyte-maturation-gamete-somatic-cells-interactions-meiotic-resumption-cytoskeletal-dynamics-and-cytoplasmic-reorganization
#20
REVIEW
Giovanni Coticchio, Mariabeatrice Dal Canto, Mario Mignini Renzini, Maria Cristina Guglielmo, Fausta Brambillasca, Diana Turchi, Paola Vittoria Novara, Rubens Fadini
BACKGROUND: In a growth phase occurring during most of folliculogenesis, the oocyte produces and accumulates molecules and organelles that are fundamental for the development of the preimplantation embryo. At ovulation, growth is followed by a phase of maturation that, although confined within a short temporal window, encompasses modifications of the oocyte chromosome complement and rearrangements of cytoplasmic components that are crucial for the achievement of developmental competence...
July 2015: Human Reproduction Update
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