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https://www.readbyqxmd.com/read/27894783/comparison-of-speed-vacuum-method-and-heat-drying-method-to-measure-brain-water-content-of-small-brain-samples
#1
Anne Sebastiani, Tobias Hirnet, Antje Jahn-Eimermacher, Serge C Thal
BACKGROUND: A reliable measurement of brain water content (wet-to-dry ratio) is an important prerequisite for conducting research on mechanisms of brain edema formation. The conventionally used oven-drying method suffers from several limitations, especially in small samples. A technically demanding and time-consuming alternative is freeze-drying. NEW METHOD: Centrifugal vacuum concentrators (e.g. SpeedVac/speed-vacuum drying) are a combination of vacuum-drying and centrifugation, used to reduce the boiling temperature...
November 25, 2016: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/27888213/coach-directed-education-is-associated-with-injury-prevention-behaviour-in-players-an-ecological-cross-sectional-study
#2
James C Brown, Sugnet Gardner-Lubbe, Michael Ian Lambert, Willem van Mechelen, Evert Verhagen
BACKGROUND/AIM: Rugby union ('rugby') presents an above-average risk of injury to participants. BokSmart is a South African nationwide intervention that aims to reduce rugby-related injuries in players. This is achieved by educating coaches and referees to improve injury behaviour of players. Thus, the aim of this study was to assess if the receipt of injury-prevention education was associated with player behaviour. METHODS: Junior (n=2279) and senior (n=1642) players, who attended merit-based South African Rugby tournaments (2008-2012), completed an anonymous questionnaire...
November 25, 2016: British Journal of Sports Medicine
https://www.readbyqxmd.com/read/27878650/ultra-high-magnetic-resonance-imaging-mri-a-potential-examination-for-deep-brain-stimulation-devices-and-the-limitation-study-concerning-mri-related-heating-injury
#3
Ying-Chuan Chen, Jun-Ju Li, Guan-Yu Zhu, Lin Shi, An-Chao Yang, Yin Jiang, Xin Zhang, Jian-Guo Zhang
Nowadays, the patients with deep brain stimulation (DBS) devices are restricted to undertake 1.5T magnetic resonance imaging (MRI) according to the guideline. Nevertheless, we conducted an experiment to test pathological change near the leads in different field-strength MRI. Twenty-four male New Zealand rabbits were assigned to Group 1 (G1, n = 6, 7.0T, DBS), Group 2 (G2, n = 6, 3.0T, DBS), Group 3 (G3, n = 6, 1.5T, DBS), and Group 4 (G4, n = 6, 1.5T, paracentesis). DBS leads were implanted in G1, G2 and G3, targeting left nucleus ventralis posterior thalami...
November 23, 2016: Neurological Sciences
https://www.readbyqxmd.com/read/27864716/single-incision-laparoscopic-surgery-increases-the-risk-of-unintentional-thermal-injury-from-the-monopolar-bovie-instrument-in-comparison-with-traditional-laparoscopy
#4
Nicole T Townsend, Edward L Jones, Doug Overbey, Bruce Dunne, Jennifer McHenry, Thomas N Robinson
BACKGROUND: Single-incision laparoscopic surgery (SILS) places multiple instruments in close, parallel proximity, an orientation that may have implications in the production of stray current from the monopolar "Bovie" instrument. The purpose of this study was to compare the energy transferred during SILS compared to traditional four-port laparoscopic surgery (TRD). METHOD: In a laparoscopic simulator, instruments were inserted via SILS or TRD setup. The monopolar generator delivered energy to a laparoscopic L-hook instrument for 5-s activations on 30-Watts coag mode...
November 18, 2016: Surgical Endoscopy
https://www.readbyqxmd.com/read/27863213/tubular-cytoplasmic-expression-of-snai1-in-renal-transplant-biopsies-a-sign-of-diseased-epithelial-phenotype
#5
Marco Prunotto, Lyubov Chaykovska, Massimo Bongiovanni, Milo Frattini, Thomas Cagarelli, Franziska Weibel, Maurizio Bruschi, Antonio Garcia de Herreros, Solange Moll
The aim of the present study was to analyze in vivo the role of SNAI1 on renal fibrosis. Unilateral ureteral obstruction injury was induced in Snai1 knockout mice. Snai1 gene deletion was, however, only partial and could therefore not be correlated to reduced fibrosis. Expression of SNAI1 protein and epithelial-mesenchymal transformation markers was then assessed in human chronic allograft nephropathy biopsy specimens. Significant up-regulation of SNAI1 protein was detected within cytoplasm of proximal tubules localized, for some of them, near foci of fibrosis and tubular atrophy...
November 15, 2016: American Journal of Pathology
https://www.readbyqxmd.com/read/27863118/nanofibrous-gelatin-scaffolds-integrated-with-ngf-loaded-alginate-microspheres-for-brain-tissue-engineering
#6
Melda Büyüköz, Esra Erdal, Sacide Alsoy Altinkaya
Neural regeneration research is designed in part to develop strategies for therapy after nerve damage due to injury or disease. In this study, a new gelatin based biomimetic scaffold was fabricated for brain tissue engineering applications. A technique combining thermally induced phase separation and porogen leaching was used to create interconnected macropores and nanofibrous structure. To promote tissue regeneration processes, the scaffolds were integrated with nerve growth factor (NGF) loaded alginate microspheres...
November 12, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27862616/topical-gabapentin-gel-alleviates-allodynia-and-hyperalgesia-in-the-chronic-sciatic-nerve-constriction-injury-neuropathic-pain-model
#7
M Shahid, F Subhan, N Ahmad, G Ali, S Akbar, K Fawad, R D E Sewell
BACKGROUND: Systemic gabapentin is a mainstay treatment for neuropathic pain though there are side-effects. Localized therapy may curtail such side-effects so a topical gabapentin dermal application was examined in the chronic constriction injury (CCI) model of neuropathic pain. METHODS: Partial denervation CCI was achieved by rat sciatic nerve ligation. Gabapentin gel (10% w/w) was applied three times daily on the ipsilateral or contralateral plantar surface of the hind-paw, whereas in a concurrent systemic study, gabapentin was intraperitoneally administered daily (75 mg/kg) for 30 days...
November 8, 2016: European Journal of Pain: EJP
https://www.readbyqxmd.com/read/27862254/hk2-proximal-tubule-epithelial-cells-synthesize-and-secrete-plasma-proteins-predominantly-through-the-apical-surface
#8
Ke-Wei Zhao, Elsa J Brochmann Murray, Samuel S Murray
Renal proximal tubule epithelial cells (PTECs) are known to reabsorb salts and small plasma proteins filtered through Bowman's capsule. Following acute kidney injury, PTECs assume some characteristics of hepatocytes in producing various plasma proteins. We now demonstrate that even at a resting state, a PTEC cell line, HK2 expresses mRNAs for and synthesizes and secretes plasma proteins in a complex with complement C3, an α2 -macroglobulin family chaperone, including albumin, transferrin, α1 -antitrypsin, α1 -antichymotrypsin, α2 -HS-glycoprotein, ceruloplasmin, haptoglobin, C1-inhibitor, secreted phosphoprotein-24, and insulin-like growth factor-1...
November 9, 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27857950/the-thermal-probe-test-a-novel-behavioral-assay-to-quantify-thermal-paw-withdrawal-thresholds-in-mice
#9
Jennifer R Deuis, Irina Vetter
Rodent models are frequently used to improve our understanding of the molecular mechanisms of pain and to develop novel analgesics. Robust behavioral assays that quantify nociceptive responses to different sensory modalities, such has heat, are therefore needed. Here, we describe a novel behavioral assay to quantify thermal paw withdrawal thresholds in mice, called the thermal probe test, and compared it with other methods commonly used to measure heat thresholds, namely the Hargreaves test and the dynamic and conventional hot plate tests...
April 2016: Temperature: Multidisciplinary Biomedical Journal
https://www.readbyqxmd.com/read/27856613/dorsal-horn-interneuron-derived-netrin-4-contributes-to-spinal-sensitization-in-chronic-pain-via-unc5b
#10
Yasufumi Hayano, Keiko Takasu, Yoshihisa Koyama, Moe Yamada, Koichi Ogawa, Kazuhisa Minami, Toshiyuki Asaki, Kazuhiro Kitada, Satoshi Kuwabara, Toshihide Yamashita
Because of the incomplete understanding of the molecular mechanisms that underlie chronic pain, the currently available treatments for this type of pain remain inefficient. In this study, we show that Netrin-4, a member of the axon guidance molecule family, was expressed in dorsal horn inner lamina II excitatory interneurons in the rat spinal cord. A similar expression pattern for Netrin-4 was also observed in human spinal cord. Behavioral analysis revealed that tactile and heat hyperalgesia after peripheral nerve injury or inflammation were abolished in Netrin-4-mutant rats...
November 17, 2016: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/27852415/-the-diagnosis-and-treatment-for-pyriform-sinus-fistula-in-children
#11
T Liu, Z Wen, Q F Liang, F Liu, Z Wang, J K Liang
Objective: To discuss the diagnosis and management for pyriform sinus fistula in children. Methods: Eight cases of pyriform sinus fistula admitted in Guangzhou Women and Children's Medical Center from May 2013 to August 2015 were retrospectively analyzed. The median age at onset was 54 months (range from 11 months to 12 years). All 8 cases presented recurrent neck infection (typical of redness, swelling, heat and pain) and 2 accompanied with acute suppurative thyroiditis. The diagnosis was confirmed by the preoperative barium esophagography combined with neck computed tomography scan (plain scan, enhancement and reconstruction)...
October 25, 2016: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/27849463/core-temperature-in-service-members-with-and-without-traumatic-amputations-during-a-prolonged-endurance-event
#12
Anne M Andrews, Christina Deehl, Reva L Rogers, Alison L Pruziner
INTRODUCTION: Service members with traumatic amputations may be at an increased risk of elevated core body temperature, since their ability to dissipate heat may decrease with the reduction in body surface area (BSA) after injury. Elevated core temperature can impair physical performance during combat operations potentially putting the service members and their teams at risk. The purpose of this study was to compare core temperature between individuals with and without amputations during a prolonged endurance event...
November 2016: Military Medicine
https://www.readbyqxmd.com/read/27849315/a-new-experimental-burn-model-with-an-infrared-heater
#13
Serbülent Güzey, Ahmet Demirhan Dal, İsmail Şahin, Mustafa Nişancı, İbrahim Yavan
BACKGROUND: This study was undertaken to develop new experimental burn injury model using conventional infrared heaters. METHODS: 21 Sprague-Dawley rats were divided into 3 groups. Portion of dorsal area was exposed to infrared radiation from distance of 50 cm to create burn injury. Length of exposure to heat for Group 1 was 5 minutes; Group 2 was exposed for 7½ minutes, and Group 3 was exposed for 10 minutes. Macroscopic and histopathological evaluations were utilized to demonstrate depth and characteristics of injury...
September 2016: Ulusal Travma Ve Acil Cerrahi Dergisi, Turkish Journal of Trauma & Emergency Surgery: TJTES
https://www.readbyqxmd.com/read/27846768/olive-oil-abrogates-acrylamide-induced-nephrotoxicity-by-modulating-biochemical-and-histological-changes-in-rats
#14
Imen Ghorbel, Awatef Elwej, Nesrine Fendri, Héla Mnif, Kamel Jamoussi, Tahia Boudawara, Naziha Grati Kamoun, Najiba Zeghal
Acrylamide (ACR) is one of the most important contaminants occurring in foods heated at high temperatures. The aim of this study is to investigate the protective efficacy of extra virgin olive oil (EVOO), a main component of the Mediterranean diet, against nephrotoxicity induced by ACR. Rats have received by gavage during 21 days either ACR (40 mg/kg body weight) or ACR-associated with EVOO (300 μl) or only EVOO (300 μl). Acrylamide induced nephrotoxicity as evidenced by an increase in malondialdehyde (MDA), hydrogen peroxide (H2O2), protein carbonyls (PCOs) and a decrease in glutathione, non-protein thiols (NPSHs), and vitamin C levels...
November 15, 2016: Renal Failure
https://www.readbyqxmd.com/read/27843858/clinical-study-of-burn-patients-requiring-admission-a-single-center-experience-at-north-eastern-indira-gandhi-regional-institute-of-health-and-medical-sciences
#15
Donkupar Khongwar, Ranendra Hajong, Jyoutishman Saikia, Noor Topno, Arup J Baruah, Ojing Komut
BACKGROUND: Although burns are a major problem in health care, a lot of the variation in risk factors exists from region to region which if uncovered correctly could help take effective prevention measures. AIMS AND OBJECTIVES: To assess the 3-year (January 2012 to January 2015) epidemiology of burn injuries admitted to our hospital (primary objective) and to find areas of improvement in burn care (secondary objective). MATERIALS AND METHODS: After obtaining ethical approval data were obtained from the medical record section regarding age, sex, residence, occupation, marital status, socioeconomic status, dates of admission and discharge, circumstances regarding the place, intent, cause, and source of heat...
April 2016: Journal of Family Medicine and Primary Care
https://www.readbyqxmd.com/read/27836236/optimising-ballistic-facial-coverage-from-military-fragmenting-munitions-a-consensus-statement
#16
J Breeze, D C Tong, D Powers, N A Martin, A M Monaghan, D Evriviades, J Combes, G Lawton, C Taylor, A Kay, J Baden, B Reed, N MacKenzie, A J Gibbons, S Heppell, R F Rickard
VIRTUS is the first United Kingdom (UK) military personal armour system to provide components that are capable of protecting the whole face from low velocity ballistic projectiles. Protection is modular, using a helmet worn with ballistic eyewear, a visor, and a mandibular guard. When all four components are worn together the face is completely covered, but the heat, discomfort, and weight may not be optimal in all types of combat. We organized a Delphi consensus group analysis with 29 military consultant surgeons from the UK, United States, Canada, Australia, and New Zealand to identify a potential hierarchy of functional facial units in order of importance that require protection...
November 8, 2016: British Journal of Oral & Maxillofacial Surgery
https://www.readbyqxmd.com/read/27835852/active-antioxidants-in-ex-vivo-examination-of-burn-wound-healing-by-means-of-ir-and-raman-spectroscopies-preliminary-comparative-research
#17
Anna Pielesz, Dorota Biniaś, Ewa Sarna, Rafał Bobiński, Marek Kawecki, Justyna Glik, Agnieszka Klama-Baryła, Diana Kitala, Wojciech Łabuś, Jadwiga Paluch, Małgorzata Kraut
Being a complex traumatic event, burn injury also affects other organ systems apart from the skin. Wounds undergo various pathological changes which are accompanied by alterations in the molecular environment. Information about molecules may be obtained with the use of Raman spectroscopy and Fourier-transform infrared spectroscopy, and when combined, both methods are a powerful tool for providing material characterization. Alterations in the molecular environment may lead to identifying objective markers of acute wound healing...
November 1, 2016: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/27831921/the-effects-of-smoking-and-nicotine-ingestion-on-exercise-heat-tolerance
#18
Amit Druayn, Danit Atias, Itay Ketko, Yoav Cohen-Sivan, Yuval Heled
BACKGROUND: Smoking has a thermogenic effect and is associated with low physical performance. Nevertheless, a direct, quantitative effect of acute smoking on exercise heat tolerance has not been reported. METHODS: Sixteen healthy young male volunteers, eight cigarette smokers, and eight non-smokers participated in the study. All subjects performed a maximal oxygen consumption test (VO2max) and a standardized heat tolerance test (HTT) after at least 12 h without smoking under the following conditions: no nicotine exposure, 10 min after nicotine exposure (2 mg nicotine lozenge), and 10 min after smoking two cigarettes (0...
November 10, 2016: Journal of Basic and Clinical Physiology and Pharmacology
https://www.readbyqxmd.com/read/27830023/over-expression-of-heat-shock-protein-70-protects-mice-against-lung-ischemia-reperfusion-injury-through-sirt1-ampk-enos-pathway
#19
Shumei Liu, Junping Xu, Chunfang Fang, Chunjing Shi, Xin Zhang, Bo Yu, Yantong Yin
Lung ischemia/reperfusion injury (LIRI) usually occurs during in lung transplantation and extracorporeal circulation operation and may develop into pulmonary infections, acute rejection and bronchiolitis obliterans syndrome. Recent studies have discovered the protective effect of heat shock protein 70 (HSP70) on various types of injuries. In the present study, we firstly explore the role of over-expressed HSP70 on the protection against LIRI. Lung Wet/Dry (W/D) ratio, biomarkers in the bronchoalveolar lavage fluid (BALF), lung histological changes and apoptosis markers, oxidative products and proinflammatory cytokines in the lung tissues were analyzed...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27826463/thermal-injury-to-reconstructed-breasts-from-commonly-used-warming-devices-a-risk-for-reconstructive-failure
#20
Heather R Faulkner, Amy S Colwell, Eric C Liao, Jonathan M Winograd, William G Austen
BACKGROUND: Sensation is decreased or absent after breast reconstruction. This leaves reconstructed breasts vulnerable to injury from common household thermal sources such as heating pads and hot water bottles. We sought to categorize these injuries, provide a treatment plan, and prevent these injuries in the future. METHODS: A retrospective review of patients who had sustained burns to reconstructed breasts with household devices was performed at a single institution...
October 2016: Plastic and Reconstructive Surgery. Global Open
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