Read by QxMD icon Read


Kunihiko Watanabe
It is becoming well-known that bacterial cells produce membrane vesicles (MVs) from the cell surface in a budding manner, whereas the detailed mechanisms of MV biogenesis remain unclear. MVs are not authentic cells, since they are observed to be between 20 and 300 nm in size but have a structure close to the subcellular compartments. In a sense, the structure of MVs containing biogenic and cellular substances and their behavior look similar to those of viruses. Due to these scientific facts, several potent applications employing MVs as a promising tool have been proposed and reported...
October 19, 2016: Applied Microbiology and Biotechnology
Noelle Merchak, Virginie Silvestre, Denis Loquet, Toufic Rizk, Serge Akoka, Joseph Bejjani
Triacylglycerols, which are quasi-universal components of food matrices, consist of complex mixtures of molecules. Their site-specific (13)C content, their fatty acid profile, and their position on the glycerol moiety may significantly vary with the geographical, botanical, or animal origin of the sample. Such variables are valuable tracers for food authentication issues. The main objective of this work was to develop a new method based on a rapid and precise (13)C-NMR spectroscopy (using a polarization transfer technique) coupled with multivariate linear regression analyses in order to quantify the whole set of individual fatty acids within triacylglycerols...
October 19, 2016: Analytical and Bioanalytical Chemistry
Mehtab Parveen, Faheem Ahmad, Ali Mohammed Malla, Shaista Azaz, Mahboob Alam, Omer A Basudan, Manuela Ramos Silva, Pedro S Pereira Silva
Phytochemical investigations of the ethanolic extract of leaves of Clutia lanceolata (Family: Euphorbiaceae) resulted in the isolation of four compounds viz. 3,4-dihydroxy-2-methylbenzoic acid (1), 2,2'-dihydroxy-1,1'-binaphthyl (2), 1,3,8-trihydroxy-6-methylanthracene-9,10-dione (3) and 5-hydroxy-1,7-bis(4-hydroxy-3-methoxyphenyl)hepta-1,4,6-trien-3-one (4). Although all the isolated compounds were known but this was the first report from this plant source. Their structures were established on the basis of chemical and physical evidences viz...
October 18, 2016: Natural Products and Bioprospecting
Thashi Chang, Bethan Lang, Angela Vincent
BACKGROUND: Stiff person syndrome is a highly disabling, progressive autoimmune disorder of the central nervous system characterized by muscle rigidity and spasms. Stiff person syndrome is rare, but is believed to be under diagnosed with only 14 cases been reported among a 1.7 billion population in South Asia. We report the first authenticated case from Sri Lanka. CASE PRESENTATION: A 55-year-old Sri Lankan female presented with difficulty in walking and recurrent falls due to progressive muscular rigidity in her lower limbs and trunk with superimposed muscle spasms that occurred in response to unexpected noise, startle or emotional upset...
October 18, 2016: BMC Research Notes
Sundar Poovitha, Nithaniyal Stalin, Raju Balaji, Madasamy Parani
The genus Hibiscus L. includes several taxa of medicinal value and species used for the extraction of natural dyes. These applications require the use of authentic plant materials. DNA barcoding is a molecular method for species identification, which helps in reliable authentication by using one or more DNA barcode marker. In this study, we have collected 44 accessions, representing 16 species of Hibiscus, distributed in the southern peninsular India, to evaluate the discriminatory power of the two core barcodes rbcLa and matK together with the suggested additional regions trnH-psbA and ITS2...
September 14, 2016: Genome Génome / Conseil National de Recherches Canada
Racha Fadlallah, Fadi El-Jardali, Farah Annan, Hayat Azzam, Elie A Akl
BACKGROUND: A recent systematic review suggested that drug registrations and onsite quality inspections may be effective in reducing the prevalence of counterfeit and substandard drugs. However, simply replicating the most effective interventions is problematic, as it denotes implementing the intervention without further adaptation. OBJECTIVE: The aim was to systematically review the evidence beyond effectiveness for systems-level interventions to combat or prevent drug counterfeiting...
2016: Pharmaceutical Medicine
Yue Cai, Zhengyu Huang, Michelle Hoi Ching Cheung, Vincent Motto-Ros, Po-Chun Chu, Yuanwei Wang, Haoyi Zhong, Ronald Yuen, Kelvin Sze-Yin Leung, Judy T S Lum, Sut Kam Phoebe Ho, Nai Ho H Cheung
Chemical analysis of Chinese black ink on xuan paper is useful for the authentication of Asian artwork. The analysis has to be non-destructive and has to accommodate artworks of all sizes. We apply three analytical techniques, ArF laser-induced plume fluorescence, Fourier transform infra-red (FTIR) spectroscopy and portable X-ray fluorescence (pXRF) to analyze five commercial Chinese black inks on two kinds of xuan paper. The FTIR signal is found to be interfered by the substrate which is inevitable because the pigments diffuse extensively into the xuan fiber network...
October 18, 2016: Analytical Chemistry
Christian Brinch Mollerup, Petur Weihe Dalsgaard, Marie Mardal, Kristian Linnet
High-resolution mass spectrometry (HRMS) is widely used for the drug screening of biological samples in clinical and forensic laboratories. With the continuous addition of new psychoactive substances (NPS), keeping such methods updated is challenging. HRMS allows for combined targeted and non-targeted screening; first, peaks are identified by software algorithms, and identifications are based on reference standard data. Remaining unknown peaks are attempted identified with in silico and literature data. However, several thousand peaks remain where most are unidentifiable or uninteresting in drug screening...
October 17, 2016: Drug Testing and Analysis
Artur Carnicer, Oriol Arteaga, Josep M Suñé-Negre, Bahram Javidi
The counterfeiting of pharmaceutical products represents concerns for both industry and the safety of the general public. Falsification produces losses to companies and poses health risks for patients. In order to detect fake pharmaceutical tablets, we propose producing film-coated tablets with gold nanoparticle encoding. These coated tablets contain unique polarimetric signatures. We present experiments to show that ellipsometric optical techniques, in combination with machine learning algorithms, can be used to distinguish genuine and fake samples...
October 1, 2016: Optics Letters
Peter Kieseberg, Bernd Malle, Peter Frühwirt, Edgar Weippl, Andreas Holzinger
The "doctor in the loop" is a new paradigm in information-driven medicine, picturing the doctor as authority inside a loop supplying an expert system with information on actual patients, treatment results, and possible additional (side-)effects, including general information in order to enhance data-driven medical science, as well as giving back treatment advice to the doctor himself. While this approach can be very beneficial for new medical approaches like P4 medicine (personal, predictive, preventive, and participatory), it also relies heavily on the authenticity of the data and thus increases the need for secure and reliable databases...
March 19, 2016: Brain Informatics
Carl Brunius, Lin Shi, Rikard Landberg
INTRODUCTION: Liquid chromatography-mass spectrometry (LC-MS) is a commonly used technique in untargeted metabolomics owing to broad coverage of metabolites, high sensitivity and simple sample preparation. However, data generated from multiple batches are affected by measurement errors inherent to alterations in signal intensity, drift in mass accuracy and retention times between samples both within and between batches. These measurement errors reduce repeatability and reproducibility and may thus decrease the power to detect biological responses and obscure interpretation...
2016: Metabolomics: Official Journal of the Metabolomic Society
Jong-Hak Kim, Min-Kyeoung Kim, Hongtao Wang, Hee-Nyeong Lee, Chi-Gyu Jin, Woo-Saeng Kwon, Deok-Chun Yang
BACKGROUND: Korean ginseng (Panax ginseng) is one of the most important medicinal plants in the Orient. Among nine cultivars of P. ginseng, Chunpoong commands a much greater market value and has been planted widely in Korea. Chunpoong has superior quality "Chunsam" (1(st) grade ginseng) when made into red ginseng. METHODS: A rapid and reliable method for discriminating the Chunpoong cultivar was developed by exploiting a single nucleotide polymorphism (SNP) in the auxin repressed protein gene of nine Korean ginseng cultivars using specific primers...
October 2016: Journal of Ginseng Research
Carl Erik Olsen, Xiao-Chen Huang, Cecilie I C Hansen, Don Cipollini, Marian Ørgaard, Annemarie Matthes, Fernando Geu-Flores, Marcus A Koch, Niels Agerbirk
As a basis for future investigations of evolutionary trajectories and biosynthetic mechanisms underlying variations in glucosinolate structures, we screened members of the crucifer tribe Cardamineae by HPLC-MS/MS, isolated and identified glucosinolates by NMR, searched the literature for previous data for the tribe, and collected HPLC-MS/MS data for nearly all glucosinolates known from the tribe as well as some related structures (70 in total). This is a considerable proportion of the approximately 142 currently documented natural glucosinolates...
October 13, 2016: Phytochemistry
Xiaojuan Zhang, Dabing Ren, Zhibiao Yi, Lunzhao Yi, Zhongquan Xin
GC-MS fingerprints of Radix Polygalae (RP) were measured for deliberately collected samples. A total of 88 volatile components were identified and quantified by subwindow factor analysis, heuristic evolving latent projection, and retention index. Next, an efficient discrimination model based on partial least-squares (PLS) discriminant analysis (DA) was developed to distinguish the superior RP samples from the inferior ones, and the reliability and predictive ability of the model was evaluated by cross-validation and permutation tests...
October 13, 2016: Journal of AOAC International
M P Turuk, A P Dhande
The exponential augmentation of information and communication technologies has appreciably shifted panorama of Health Information System (HIS), drawing keen interests of the researchers to address the challenges in protecting the medical data. Digital Watermarking therefore gained wide popularity as an entrusted measure to ensure the security. Literature reports wide assortment of imaging approaches for digital watermarking; however its perspectives in the HIS have received meager attention, especially in the reversible watermarking context...
December 2016: Journal of Medical Systems
Piotr Kowalski, Ilona Olędzka, Alina Plenis, Tomasz Bączek
An indirect UV detection method based on capillary electrophoresis was developed and validated to determinate 12. metal cations, including alkali, alkaline earth, transition metal, and ammonium. In this paper a new electrolyte system (pH 4.22) contained 20 mM benzimidazole (as co-ion), 75 mM acetic acid (as a counter-ion) as well as 0.6 mM 18-crown-6 ether was applied. The metal ions were completely separated within 8 min under hydrodynamic mode injection with a running voltage of 20 kV at 25 ± 0.1°C. Additional use of the dynamic double coating method enabled to get an excellent repeatability of migration times and quantitative parameters for all analytes...
October 14, 2016: Electrophoresis
Youngseok Chung, Seokjin Choi, Youngsook Lee, Namje Park, Dongho Won
More security concerns and complicated requirements arise in wireless sensor networks than in wired networks, due to the vulnerability caused by their openness. To address this vulnerability, anonymous authentication is an essential security mechanism for preserving privacy and providing security. Over recent years, various anonymous authentication schemes have been proposed. Most of them reveal both strengths and weaknesses in terms of security and efficiency. Recently, Farash et al. proposed a lightweight anonymous authentication scheme in ubiquitous networks, which remedies the security faults of previous schemes...
October 7, 2016: Sensors
Andrea E Steuer, Elena Williner, Sandra Staeheli, Thomas Kraemer
Increasing numbers of new psychoactive substances (NPS) among them fentanyl derivatives has been reported by the European monitoring centre for drugs and drug addiction (EMCDDA). Butyrfentanyl is a new fentanyl derivative whose potency ratio was found to be seven compared to morphine and 0.13 compared to fentanyl. Several case reports on butyrfentanyl intoxications have been described. Little is known about its pharmacokinetic properties including its metabolism. However, knowledge of metabolism is essential for analytical detection in clinical and forensic toxicology...
October 13, 2016: Drug Testing and Analysis
Xiong Li, Jianwei Niu, Marimuthu Karuppiah, Saru Kumari, Fan Wu
Benefited from the development of network and communication technologies, E-health care systems and telemedicine have got the fast development. By using the E-health care systems, patient can enjoy the remote medical service provided by the medical server. Medical data are important privacy information for patient, so it is an important issue to ensure the secure of transmitted medical data through public network. Authentication scheme can thwart unauthorized users from accessing services via insecure network environments, so user authentication with privacy protection is an important mechanism for the security of E-health care systems...
December 2016: Journal of Medical Systems
Alana M Thackray, Olivier Andreoletti, Raymond Bujdoso
In pursuit of a tractable bioassay to assess blood prion infectivity we have generated PrP transgenic Drosophila , which show a neurotoxic phenotype in adulthood after exposure to exogenous prions at the larval stage. Here we determined the sensitivity of ovine PrP transgenic Drosophila to ovine prion infectivity by exposure of these flies to a dilution series of scrapie-infected sheep brain homogenate. Ovine PrP transgenic Drosophila showed a significant neurotoxic response to dilutions of 10-2 through to 10-10 of the original scrapie-infected sheep brain homogenate...
October 12, 2016: Biochemical Journal
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"