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https://www.readbyqxmd.com/read/29684910/forensic-applications-of-supercritical-fluid-chromatography-mass-spectrometry
#1
REVIEW
Volodymyr Pauk, Karel Lemr
Achievements of supercritical fluid chromatography with mass spectrometric detection made in the field of forensic science during the last decade are reviewed. The main topics include analysis of traditional drugs of abuse (e.g. cannabis, methamphetamine) as well as new psychoactive substances (synthetic cannabinoids, cathinones and phenethylamines), doping agents (anabolic steroids, stimulants, diuretics, analgesics etc.) and chemical warfare agents. Control of food authenticity, detection of adulteration and identification of toxic substances in food are also pointed out...
April 11, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29684833/antecedents-mediators-and-outcomes-of-authentic-leadership-in-healthcare-a-systematic-review
#2
REVIEW
Bayan Alilyyani, Carol A Wong, Greta Cummings
BACKGROUND: Leaders are essential in every organization to achieve patient safety and healthy work environments. Authentic leadership is a relational leadership style purported to promote healthy work environments that influence staff performance and organizational outcomes. Given recent growth in authentic leadership research in healthcare and the importance of new knowledge to inform leadership development, there is an obligation to determine what is known about the antecedents and outcomes of authentic leadership in healthcare settings and clarify mechanisms by which authentic leadership affects healthcare staff and patient outcomes...
April 7, 2018: International Journal of Nursing Studies
https://www.readbyqxmd.com/read/29683355/a-novel-pcr-based-technology-for-rapid-and-non-sequencing-authentication-of-bombyx-batryticatus-using-species-specific-primers
#3
Huan Yang, Ying Zhou, Pingtian Yu, Yaya Yang, Zhaoqun Jiao, James P Tam, Yuping Shen, Xiaobin Jia
A novel PCR technology was developed to detect short DNA fragments using species-specific primers for rapid and non-sequencing authentication of Bombyx batryticatus based on differences in the mitochondrial genome. Three specifically designed primer reactions were established to target species for the reliable identification of their commercial products. They were confirmed to have a high inter-species specificity and intra-species stability. The limit of detection was estimated as 1 ng of genomes for Beauveria bassiana and 100 pg for Bombyx mori and Metarhizium anisopliae...
April 23, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29682301/ethical-challenges-in-developing-an-educational-video-to-empower-potential-participants-during-consent-processes-in-hiv-cure-research-in-south-africa
#4
EDITORIAL
Ciara Staunton, Malcolm de Roubaix, Dianno Baatjies, Gill Black, Melany Hendricks, Theresa Rossouw, Keymanthri Moodley
Obtaining consent for HIV research is complex, particularly in low- and middle-income countries. Low levels of education, complexity of science and research processes, confusion about basic elements of research, and socio-economic conditions that make access to medical care difficult have collectively led to concerns about the adequacy of the consent process. Given the exponential growth of HIV prevention and treatment research in South Africa, HIV researchers are increasingly facing challenges obtaining authentic informed consent from potential participants...
April 1, 2018: Journal of Virus Eradication
https://www.readbyqxmd.com/read/29681888/octreotide-does-not-inhibit-proliferation-in-five-neuroendocrine-tumor-cell-lines
#5
Samantha Exner, Vikas Prasad, Bertram Wiedenmann, Carsten Grötzinger
Somatostatin analogs (SSA) are well-established antisecretory drugs in functionally active neuroendocrine tumors (NET). Two placebo-controlled trials have recently demonstrated significant improvement of progression-free survival under SSA treatment. Furthermore, somatostatin receptor (SSTR) overexpression in NET has also been utilized for diagnostic imaging and peptide receptor radionuclide therapy (PRRT). However, PRRT in NET is associated mostly with partial and minor remission, while other radionuclide therapies reach complete remissions in up to 75% of cases...
2018: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29681758/ozonolysis-of-%C3%AE-%C3%AE-farnesene-mixture-analysis-of-gas-phase-and-particulate-reaction-products
#6
Mohammed Jaoui, Michael Lewandowski, John H Offenberg, Kenneth S Docherty, Tadeusz E Kleindienst
Atmospheric oxidation of sesquiterpenes has been of considerable interest recently because of their likely contribution to ambient organic aerosol, but farnesene oxidation has been reported in only a few studies and with limited data. In the present study, a detailed chemical analysis of the organic fraction of gas and particle phases originating from the ozonolysis of a mixture of α-farnesene and β-farnesene was carried out in a 14.5 m3 smog chamber. More than 80 organic compounds bearing OH functionality were detected for the first time in this system in the gas and particle phases...
November 2017: Atmospheric Environment
https://www.readbyqxmd.com/read/29680139/-trust-in-the-care-relationship
#7
Patrick Sureau
A relationship of trust is an expression often used by caregivers, to such an extent that it almost seems self-evident. It is nevertheless important to give some thought to this aspect in order to construct a reliable, authentic and ethical care relationship. Indeed, trust is not automatic. It requires reciprocity, a deliberate choice on the part of the caregiver and the patient.
April 2018: Soins; la Revue de Référence Infirmière
https://www.readbyqxmd.com/read/29676614/sourcebook-update-intestinal-smooth-muscle-contractility-and-autonomic-control
#8
Philip D Langton
This laboratory practical requires first-year students to anticipate the effects of drugs active at cholinergic and adrenergic receptors on gut motility in order to design experiments during an authentic inquiry exercise. Rather than specifying a strict sequence of drug additions that aim to provide ideal demonstrations of pharmacological and physiological antagonism, I have instead designed switches into the drugs provided and set students, working in small teams, the task of identifying the switched drugs, an inquiry activity...
June 1, 2018: Advances in Physiology Education
https://www.readbyqxmd.com/read/29676141/-design-and-implementation-of-xinshenghua-granule-traceability-system-based-on-data-authenticity
#9
Zi-Bo Shen, Yang Lu, Sheng-Liang Huang, Chong Liu, Cun-Yu Li, Yun-Feng Zheng, Guo-Ping Peng
The quality of traditional Chinese medicine affects the clinical efficacy and the market research at home or abroad, which is closely related to the standardization of production. At present, Chinese market still exist in drug quality and nonstandard production phenomenon. In addition to the existing GMP production standards, it is still necessary to ensure the authenticity and reliability of traditional Chinese medicine by means of retrospect. Based on the Xinshenghua granule, starting from the authenticity of the whole process of production, this study designs and constructs the traditional Chinese medicine traceability system which is equipped with the Internet platform by using two-dimensional code as a trace tool...
March 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29675903/rare-earth-elements-concentration-in-mushroom-cultivation-substrates-affects-the-production-process-and-fruit-bodies-content-of-pleurotus-ostreatus-and-cyclocybe-cylindracea
#10
Georgios Koutrotsios, Georgios P Danezis, Constantinos A Georgiou, Georgios I Zervakis
BACKGROUND: Concentrations of 16 rare earth elements (REEs) and two actinides were determined for the first time both in cultivated mushrooms and in their production substrates by inductively coupled plasma mass spectroscopy. Moreover, REEs effect on cultivation parameters and composition of the final product was assessed, together with their potential use for authentication purposes. RESULTS: REEs concentrations presented high variability among seven cultivation substrates, and correlated with respective measurements in Cyclocybe cylindracea mushrooms; no such correlation was established in Pleurotus ostreatus...
April 20, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29675598/design-of-a-biorthogonal-wavelet-transform-based-r-peak-detection-and-data-compression-scheme-for-implantable-cardiac-pacemaker-systems
#11
Ashish Kumar, Manjeet Kumar, Rama Komaragiri
Bradycardia can be modulated using the cardiac pacemaker, an implantable medical device which sets and balances the patient's cardiac health. The device has been widely used to detect and monitor the patient's heart rate. The data collected hence has the highest authenticity assurance and is convenient for further electric stimulation. In the pacemaker, ECG detector is one of the most important element. The device is available in its new digital form, which is more efficient and accurate in performance with the added advantage of economical power consumption platform...
April 19, 2018: Journal of Medical Systems
https://www.readbyqxmd.com/read/29675032/fspp-a-tool-for-genome-wide-prediction-of-smorf-encoded-peptides-and-their-functions
#12
Hui Li, Li Xiao, Lili Zhang, Jiarui Wu, Bin Wei, Ninghui Sun, Yi Zhao
smORFs are small open reading frames of less than 100 codons. Recent low throughput experiments showed a lot of smORF-encoded peptides (SEPs) played crucial rule in processes such as regulation of transcription or translation, transportation through membranes and the antimicrobial activity. In order to gather more functional SEPs, it is necessary to have access to genome-wide prediction tools to give profound directions for low throughput experiments. In this study, we put forward a functional smORF-encoded peptides predictor (FSPP) which tended to predict authentic SEPs and their functions in a high throughput method...
2018: Frontiers in Genetics
https://www.readbyqxmd.com/read/29674023/evaluation-of-portable-near-infrared-spectroscopy-for-organic-milk-authentication
#13
Ningjing Liu, Hector Aya Parra, Annemieke Pustjens, Kasper Hettinga, Philippe Mongondry, Saskia M van Ruth
Organic products are vulnerable to fraud due to their premium price. Analytical methodology helps to manage the risk of fraud and due to the miniaturization of equipment, tests may nowadays even be rapidly applied on-site. The current study aimed to evaluate portable near infrared spectroscopy (NIRS) in combination with chemometrics to distinguish organic milk from other types of milk, and compare its performance with benchtop NIRS and fatty acid profiling by gas chromatography. The sample set included 37 organic retail milks and 50 non-organic retail milks (of which 36 conventional and 14 green 'pasture' milks)...
July 1, 2018: Talanta
https://www.readbyqxmd.com/read/29670150/visualizing-multiple-inter-organelle-contact-sites-using-the-organelle-targeted-split-gfp-system
#14
Yuriko Kakimoto, Shinya Tashiro, Rieko Kojima, Yuki Morozumi, Toshiya Endo, Yasushi Tamura
Functional integrity of eukaryotic organelles relies on direct physical contacts between distinct organelles. However, the entity of organelle-tethering factors is not well understood due to lack of means to analyze inter-organelle interactions in living cells. Here we evaluate the split-GFP system for visualizing organelle contact sites in vivo and show its advantages and disadvantages. We observed punctate GFP signals from the split-GFP fragments targeted to any pairs of organelles among the ER, mitochondria, peroxisomes, vacuole and lipid droplets in yeast cells, which suggests that these organelles form contact sites with multiple organelles simultaneously although it is difficult to rule out the possibilities that these organelle contacts sites are artificially formed by the irreversible associations of the split-GFP probes...
April 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29669840/arenaviral-nucleoproteins-suppress-pact-induced-augmentation-of-rig-i-function-to-inhibit-type-i-interferon-production
#15
Junjie Shao, Qinfeng Huang, Xiaoying Liu, Da Di, Yuying Liang, Hinh Ly
RIG-I is a major cytoplasmic sensor of viral pathogen-associated molecular pattern (PAMP) RNA and induces the production of type I interferon (IFN) upon viral infection. A dsRNA-binding protein PACT plays an important role in potentiating RIG-I function. We have previously shown that arenaviral nucleoproteins (NPs) suppress type I IFN production via its exoribonuclease (RNase) activity to degrade PAMP RNA. We report here that NPs of arenaviruses block PACT-induced enhancement of RIG-I function to mediate type I IFN production and that the inhibition is dependent on the NP's RNase function, which is different than a known mechanism of other viral proteins to disrupt PACT and RIG-I interaction...
April 18, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29669839/a-gxxxa-motif-in-the-transmembrane-domain-of-the-ebola-virus-glycoprotein-is-required-for-tetherin-antagonism
#16
Mariana González-Hernández, Markus Hoffmann, Constantin Brinkmann, Julia Nehls, Michael Winkler, Michael Schindler, Stefan Pöhlmann
The interferon-induced antiviral host cell protein tetherin can inhibit the release of several enveloped viruses from infected cells. The Ebola virus (EBOV) glycoprotein (GP) antagonizes tetherin but the domains and amino acids in GP that are required for tetherin antagonism have not been fully defined. A GXXXA motif within the transmembrane domain (TMD) of EBOV-GP was previously shown to be important for GP-mediated cellular detachment. Here, we investigated whether this motif also contributes to tetherin antagonism...
April 18, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29668756/on-the-security-of-consumer-wearable-devices-in-the-internet-of-things
#17
Hasan Tahir, Ruhma Tahir, Klaus McDonald-Maier
Miniaturization of computer hardware and the demand for network capable devices has resulted in the emergence of a new class of technology called wearable computing. Wearable devices have many purposes like lifestyle support, health monitoring, fitness monitoring, entertainment, industrial uses, and gaming. Wearable devices are hurriedly being marketed in an attempt to capture an emerging market. Owing to this, some devices do not adequately address the need for security. To enable virtualization and connectivity wearable devices sense and transmit data, therefore it is essential that the device, its data and the user are protected...
2018: PloS One
https://www.readbyqxmd.com/read/29667573/molecular-epidemiology-of-extraintestinal-pathogenic-escherichia-coli
#18
James R Johnson, Thomas A Russo
Extraintestinal pathogenic Escherichia coli (ExPEC) are important pathogens in humans and certain animals. Molecular epidemiological analyses of ExPEC are based on structured observations of E. coli strains as they occur in the wild. By assessing real-world phenomena as they occur in authentic contexts and hosts, they provide an important complement to experimental assessment. Fundamental to the success of molecular epidemiological studies are the careful selection of subjects and the use of appropriate typing methods and statistical analysis...
April 2018: EcoSal Plus
https://www.readbyqxmd.com/read/29667505/a-comprehensive-screening-shows-that-ergothioneine-is-the-most-abundant-antioxidant-in-the-wild-macrofungus-phylloporia-ribis-ryvarden
#19
Hengqiang Zhao, Minmin Zhang, Qian Liu, Xiaoli Wang, Ruixuan Zhao, Yanling Geng, Tityee Wong, Shengbo Li, Xiao Wang
The polar and non-polar extracts from the authenticated wild mushroom Phylloporia ribis were separated by hydrophilic interaction liquid chromatography (HILIC) and by reverse phase (RP)-HPLC, respectively. A split valve separated the eluents into two fractions for free-radical scavenging analysis and for structural identification. Forty-six compounds showed scavenging activity of the stable-free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). The structures of 8 antioxidants (inosine, caffeic acid, ergothioneine, p-hydroxybenzoic acid, adenosine, 3,4-dihydroxybenzaldehyde, apigenin, and naringenin) are characterized by Mass Spectrometer...
April 3, 2018: Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews
https://www.readbyqxmd.com/read/29666764/evaluation-of-a-therapy-for-idiopathic-chronic-enterocolitis-in-rhesus-macaques-macaca-mulatta-and-linked-microbial-community-correlates
#20
Joshua M Taylor, Erik L Clarke, Kate Baker, Abigail Lauder, Dorothy Kim, Aubrey Bailey, Gary D Wu, Ronald G Collman, Lara Doyle-Meyers, Kasi Russell-Lodrigue, James Blanchard, Frederic D Bushman, Rudolf Bohm
Idiopathic chronic enterocolitis (ICE) is one of the most commonly encountered and difficult to manage diseases of captive rhesus macaques ( Macaca mulatta ). The etiology is not well understood, but perturbations in gut microbial communities have been implicated. Here we evaluated the effects of a 14-day course of vancomycin, neomycin, and fluconazole on animals affected with ICE, comparing treated, untreated, and healthy animals. We performed microbiome analysis on duodenal and colonic mucosal samples and feces in order to probe bacterial and/or fungal taxa potentially associated with ICE...
2018: PeerJ
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