keyword
MENU ▼
Read by QxMD icon Read
search

irreproducibility

keyword
https://www.readbyqxmd.com/read/28414712/the-need-for-a-worldwide-consensus-for-cell-line-authentication-experience-implementing-a-mandatory-requirement-at-the-international-journal-of-cancer
#1
Norbert E Fusenig, Amanda Capes-Davis, Franca Bianchini, Sherryl Sundell, Peter Lichter
Cell lines are used in life science research worldwide as biological surrogates. All cell lines are subject to major limitations when used as research tools, including (i) cross-contamination with other cells cultured in the same laboratory environment and (ii) evolution in vitro that renders a given cell line inappropriate as a surrogate for a specific biological hypothesis. There is ample evidence that cross-contamination or phenotypic drift of cells in culture can generate irreproducible or misleading data...
April 2017: PLoS Biology
https://www.readbyqxmd.com/read/28405182/a-standardized-protocol-for-stereotaxic-intrahippocampal-administration-of-kainic-acid-combined-with-electroencephalographic-seizure-monitoring-in-mice
#2
Pascal Bielefeld, Amanda Sierra, Juan M Encinas, Mirjana Maletic-Savatic, Anne Anderson, Carlos P Fitzsimons
Lack of scientific reproducibility is a growing concern and weak experimental practices may contribute to irreproducibility. Here, we describe an optimized and versatile protocol for stereotaxic intrahippocampal administration of Kainic Acid (KA) in mice with a C57Bl6 background. In this protocol, KA administration is combined with in vivo recording of neuronal activity with wired and wireless setups. Following our protocol, KA administration results in a robust dose-dependent induction of low-level epileptiform activity or Status Epilepticus (SE) and induces previously characterized hallmarks of seizure-associated pathology...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28373349/methodological-rigor-in-preclinical-cardiovascular-studies-targets-to-enhance-reproducibility-and-promote-research-translation
#3
F Daniel Ramirez, Pouya Motazedian, Richard G Jung, Pietro Di Santo, Zachary D MacDonald, Robert Moreland, Trevor Simard, Aisling A Clancy, Juan Russo, Vivian A Welch, George A Wells, Benjamin Hibbert
Rationale: Methodological sources of bias and suboptimal reporting contribute to irreproducibility in preclinical science and may negatively impact research translation. Randomization, blinding, sample size estimation, and considering sex as a biological variable are deemed crucial study design elements to maximize the quality and predictive value of preclinical experiments. Objective: To examine the prevalence and temporal patterns of recommended study design element implementation in preclinical cardiovascular research...
April 3, 2017: Circulation Research
https://www.readbyqxmd.com/read/28362402/evaporation-reducing-culture-condition-increases-the-reproducibility-of-multicellular-spheroid-formation-in-microtiter-plates
#4
Viswanath Das, Tomáš Fürst, Soňa Gurská, Petr Džubák, Marián Hajdúch
Tumor models that closely imitate in vivo conditions are becoming increasingly popular in drug discovery and development for the screening of potential anti-cancer drugs. Multicellular tumor spheroids (MCTSes) effectively mimic the physiological conditions of solid tumors, making them excellent in vitro models for lead optimization and target validation. Out of the various techniques available for MCTS culture, the liquid-overlay method on agarose is one of the most inexpensive methods for MCTS generation. However, the reliable transfer of MCTS cultures using liquid-overlay for high-throughput screening may be compromised by a number of limitations, including the coating of microtiter plates (MPs) with agarose and the irreproducibility of uniform MCTS formation across wells...
March 7, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28360252/state-of-reporting-of-primary-biomedical-research-a-scoping-review-protocol
#5
Guowei Li, Lawrence Mbuagbaw, Zainab Samaan, Yanling Jin, Ikunna Nwosu, Mitchell A H Levine, Jonathan D Adachi, Lehana Thabane
INTRODUCTION: Incomplete or inconsistent reporting remains a major concern in the biomedical literature. Incomplete or inconsistent reporting may yield the published findings unreliable, irreproducible or sometimes misleading. In this study based on evidence from systematic reviews and surveys that have evaluated the reporting issues in primary biomedical studies, we aim to conduct a scoping review with focuses on (1) the state-of-the-art extent of adherence to the emerging reporting guidelines in primary biomedical research, (2) the inconsistency between protocols or registrations and full reports and (3) the disagreement between abstracts and full-text articles...
March 29, 2017: BMJ Open
https://www.readbyqxmd.com/read/28350103/-retracted-nuclear-import-of-prototype-foamy-virus-transactivator-bel1-is-mediated-by-kpna1-kpna6-and-kpna7
#6
Jihui Duan, Zhiqin Tang, Hong Mu, Guojun Zhang
We would like to retract the article entitled "Nuclear import of prototype foamy virus transactivator Bel1 is mediated by KPNA1, KPNA6 and KPNA7" published in International Journal of Molecular Medicine 38: 339-406, 2016. The results presented in Fig. 6 have been demonstrated to be unreproducible, and we cannot provide an explanation for this. Furthermore, we have recently identified that the cell cultures used in our experiments were partly contaminated with Mycoplasma, which could have contributed to the irreproducibility of the results...
January 17, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28343685/evaluation-of-sources-of-irreproducibility-of-retention-indices-under-programmed-temperature-gas-chromatography-conditions
#7
Liejun Wu, Ii Kyu Cho, Yanjie Li, Guangxiang Zhang, Qing X Li
Retention index under the programmed temperature gas chromatography (PTGC) conditions (I(T)) is widely accepted despite of its unsatisfactory reproducibility. Effects of heating rate (rT), stationary liquid film thickness (df), inlet pressure (pi), initial temperature (To) and polarity of stationary phase (sp) on I(T) reproducibility were studied in the present work. We found that excellent reproducibility and regularity of I(T) values are obtainable at conditions of relatively low To and relatively high rT and pi, as well as use of quality thick-film column under an operation in a single-retention behavior region (i...
April 28, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28342878/3d-printing-for-the-design-and-fabrication-of-polymer-based-gradient-scaffolds
#8
Laura G Bracaglia, Brandon T Smith, Emma Watson, Navein Arumugasaamy, Antonios G Mikos, John P Fisher
To accurately mimic the native tissue environment, tissue engineered scaffolds often need to have a highly controlled and varied display of three-dimensional (3D) architecture and geometrical cues. Additive manufacturing in tissue engineering has made possible the development of complex scaffolds that mimic the native tissue architectures. As such, architectural details that were previously unattainable or irreproducible can now be incorporated in an ordered and organized approach, further advancing the structural and chemical cues delivered to cells interacting with the scaffold...
March 22, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28328894/laboratory-environmental-factors-and-pain-behavior-the-relevance-of-unknown-unknowns-to-reproducibility-and-translation
#9
Jeffrey S Mogil
The poor record of basic-to-clinical translation in recent decades has led to speculation that preclinical research is "irreproducible", and this irreproducibility in turn has largely been attributed to deficiencies in reporting and statistical practices. There are, however, a number of other reasonable explanations of both poor translation and difficulties in one laboratory replicating the results of another. This article examines these explanations as they pertain to preclinical pain research. I submit that many instances of apparent irreproducibility are actually attributable to interactions between the phenomena and interventions under study and "latent" environmental factors affecting the rodent subjects...
March 22, 2017: Lab Animal
https://www.readbyqxmd.com/read/28295910/an-analysis-of-nitroxide-based-distance-measurements-in-cell-extract-and-in-cell-by-pulsed-esr-spectroscopy
#10
Sunil Kumar Saxena, Matthew J Lawless, Amit Shimshi, Timothy F Cunningham, Monica N Kinde, Pei Tang
Distance measurements in-cell by pulsed ESR present tremendous opportunities to study proteins in a more native environment that is irreproducible in vitro. However, the in-cell environment is harsh towards the typical nitroxide radical used in DEER experiments. We perform a systematic examination of the loss of the DEER signal including contributions from nitroxide decay and nitroxide side chain cleavage. In addition, we examine whether the lifetime of the nitroxide radical can be extended by use of an oxidizing agent...
March 13, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28279477/the-evolution-of-differentiated-thyroid-cancer
#11
Sylvia L Asa
Differentiated thyroid tumours of follicular cell derivation have traditionally been classified based on architecture, cytology, or both. The features that distinguish the various entities are controversial and diagnostic criteria are inconsistent and often irreproducible. The complexity of classification schemes has not been substantiated by molecular profiling. In this review, a simplistic approach to the diagnosis of well differentiated thyroid neoplasia is provided to challenge the dogma. The proposed classification matches the molecular profiles of these lesions and simplifies the criteria for diagnosis...
March 6, 2017: Pathology
https://www.readbyqxmd.com/read/28266620/two-dimensional-electrophysiological-characterization-of-human-pluripotent-stem-cell-derived-cardiomyocyte-system
#12
Huanqi Zhu, Kelsey S Scharnhorst, Adam Z Stieg, James K Gimzewski, Itsunari Minami, Norio Nakatsuji, Haruko Nakano, Atsushi Nakano
Stem cell-derived cardiomyocytes provide a promising tool for human developmental biology, regenerative therapies, disease modeling, and drug discovery. As human pluripotent stem cell-derived cardiomyocytes remain functionally fetal-type, close monitoring of electrophysiological maturation is critical for their further application to biology and translation. However, to date, electrophysiological analyses of stem cell-derived cardiomyocytes has largely been limited by biologically undefined factors including 3D nature of embryoid body, sera from animals, and the feeder cells isolated from mouse...
March 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28257086/high-throughput-screening-methodology-to-identify-alpha-synuclein-aggregation-inhibitors
#13
Jordi Pujols, Samuel Peña-Díaz, María Conde-Giménez, Francisca Pinheiro, Susanna Navarro, Javier Sancho, Salvador Ventura
An increasing number of neurodegenerative diseases are being found to be associated with the abnormal accumulation of aggregated proteins in the brain. In Parkinson's disease, this process involves the aggregation of alpha-synuclein (α-syn) into intraneuronal inclusions. Thus, compounds that inhibit α-syn aggregation represent a promising therapeutic strategy as disease-modifying agents for neurodegeneration. The formation of α-syn amyloid aggregates can be reproduced in vitro by incubation of the recombinant protein...
March 2, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28254630/hydrodynamic-gene-delivery-in-human-skin-using-a-hollow-microneedle-device
#14
M Dul, M Stefanidou, P Porta, J Serve, C O'Mahony, B Malissen, S Henri, Y Levin, E Kochba, F S Wong, C Dayan, S A Coulman, J C Birchall
Microneedle devices have been proposed as a minimally invasive delivery system for the intradermal administration of nucleic acids, both plasmid DNA (pDNA) and siRNA, to treat localised disease or provide vaccination. Different microneedle types and application methods have been investigated in the laboratory, but limited and irreproducible levels of gene expression have proven to be significant challenges to pre-clinical to clinical progression. This study is the first to explore the potential of a hollow microneedle device for the delivery and subsequent expression of pDNA in human skin...
February 27, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28207468/significant-differences-in-the-bone-of-an-isogenic-inbred-versus-nonisogenic-outbred-murine-mandible-a-study-in-rigor-and-reproducibility
#15
Edward G Carey, Sagar S Deshpande, Kevin M Urlaub, Alexander R Zheutlin, Noah S Nelson, Alexis Donneys, Stephen Y Kang, Kathleen K Gallagher, Peter A Felice, Catherine N Tchanque-Fossuo, Steven R Buchman
Inattention to differences between animal strains is a potential cause of irreproducibility of basic science investigations. Accordingly, the authors' laboratory sought to ensure that cross-comparisons of results generated from studies of mandibular physiology utilizing the Sprague Dawley and Lewis rat strains are valid. The authors specifically investigated baseline histomorphometrics, bone mineral density, and biomechanical strength of the unaltered endogenous mandibles of the inbred, isogenic Lewis rat, and the outbred, nonisogenic Sprague Dawley rat to determine if they are indeed equal...
February 15, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28151827/ethics-of-research-for-patients-in-pain
#16
David B Waisel
PURPOSE OF REVIEW: This review describes advances in rising and continuing ethical issues in research in patients in pain. Although some of the issues focus directly on pain research, such as research in neonatal pain management, others focus on widespread ethical issues that are relevant to pain research, such as scientific misconduct, deception, placebo use and genomics. RECENT FINDINGS: Scientific misconduct is more widespread than realized and requires greater awareness of the markers of misconduct like irreproducibility...
April 2017: Current Opinion in Anaesthesiology
https://www.readbyqxmd.com/read/28144217/subtle-scientific-fallacies-undermine-the-validity-of-neuroendocrinological-research-do-not-draw-premature-conclusions-on-the-role-of-female-sex-hormones
#17
Michael P Hengartner
Major scientific flaws such as reporting and publication biases are well documented, even though acknowledgment of their importance appears to be lacking in various psychological and medical fields. Subtle and less obvious biases including selective reviews of the literature and empirically unsupported conclusions and recommendations have received even less attention. Using the literature on the association between transition to menopause, hormones and the onset of depression as a guiding example, I outline how such scientific fallacies undermine the validity of neuroendocrinological research...
2017: Frontiers in Behavioral Neuroscience
https://www.readbyqxmd.com/read/28123930/irreproducibility-of-published-bioscience-research-diagnosis-pathogenesis-and-therapy
#18
Jeffrey S Flier
No abstract text is available yet for this article.
January 2017: Molecular Metabolism
https://www.readbyqxmd.com/read/28121685/predatory-publishing-an-emerging-threat-to-the-medical-literature
#19
COMMENT
H Benjamin Harvey, Debra F Weinstein
The quality of medical literature is increasingly threatened by irresponsible publishing, leading to rising retraction rates, irreproducible results, and a flood of inconsequential publications that distract readers from more meaningful scholarship. "Predatory publishers" offer rapid publication with loose peer review, exploiting a system in which faculty seek longer bibliographies to achieve academic promotion. In this Commentary, the authors highlight some of the evidence that this problem exists and suggest actions to address it...
February 2017: Academic Medicine: Journal of the Association of American Medical Colleges
https://www.readbyqxmd.com/read/28117563/patent-law-s-reproducibility-paradox
#20
Jacob S Sherkow
Clinical research faces a reproducibility crisis. Many recent clinical and preclinical studies appear to be irreproducible--their results cannot be verified by outside researchers. This is problematic for not only scientific reasons but also legal ones: patents grounded in irreproducible research appear to fail their constitutional bargain of property rights in exchange for working disclosures of inventions. The culprit is likely patent law’s doctrine of enablement. Although the doctrine requires patents to enable others to make and use their claimed inventions, current difficulties in applying the doctrine hamper or even actively dissuade reproducible data in patents...
January 2017: Duke Law Journal
keyword
keyword
84458
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"