keyword
https://read.qxmd.com/read/31915261/osteoclasts-derive-predominantly-from-bone-marrow-resident-cx-3-cr1-precursor-cells-in-homeostasis-whereas-circulating-cx-3-cr1-cells-contribute-to-osteoclast-development-during-fracture-repair
#21
JOURNAL ARTICLE
Sanja Novak, Emilie Roeder, Judith Kalinowski, Sandra Jastrzebski, Hector L Aguila, Sun-Kyeong Lee, Ivo Kalajzic, Joseph A Lorenzo
Osteoclasts (OC) originate from either bone marrow (BM)-resident or circulating myeloid OC progenitors (OCP) expressing the receptor CX3 CR1. Multiple lines of evidence argue that OCP in homeostasis and inflammation differ. We investigated the relative contributions of BM-resident and circulating OCP to osteoclastogenesis during homeostasis and fracture repair. Using CX3 CR1-EGFP/TRAP tdTomato mice, we found CX3 CR1 expression in mononuclear cells, but not in multinucleated TRAP+ OC. However, CX3 CR1 - expressing cells generated TRAP+ OC on bone within 5 d in CX3 CR1CreERT2/Ai14 tdTomato reporter mice...
January 8, 2020: Journal of Immunology
https://read.qxmd.com/read/31859429/engraftment-of-skeletal-progenitor-cells-by-bone-directed-transplantation-improves-osteogenesis-imperfecta-murine-bone-phenotype
#22
JOURNAL ARTICLE
Benjamin P Sinder, Sanja Novak, Natalie K Y Wee, Mariangela Basile, Peter Maye, Brya G Matthews, Ivo Kalajzic
Osteogenesis imperfecta (OI) is a genetic disorder most commonly caused by mutations associated with type I collagen, resulting in a defective collagen bone matrix. Current treatments for OI focus on pharmaceutical strategies to increase the amount of defective bone matrix, but do not address the underlying collagen defect. Introducing healthy donor stem cells that differentiate into osteoblasts producing normal collagen in OI patients has the potential to increase bone mass and correct the mutant collagen matrix...
April 2020: Stem Cells
https://read.qxmd.com/read/31808575/ctrp3-regulates-endochondral-ossification-and-bone-remodeling-during-fracture-healing
#23
JOURNAL ARTICLE
Daniel W Youngstrom, Robert L Zondervan, Nicole R Doucet, Parker K Acevedo, Hannah E Sexton, Emily A Gardner, JonCarlos S Anderson, Priyanka Kushwaha, Hannah C Little, Susana Rodriguez, Ryan C Riddle, Ivo Kalajzic, G William Wong, Kurt D Hankenson
C1q/TNF-related protein 3 (CTRP3) is a cytokine known to regulate a variety of metabolic processes. Though previously undescribed in the context of bone regeneration, high throughput gene expression experiments in mice identified CTRP3 as one of the most highly upregulated genes in fracture callus tissue. Hypothesizing a positive regulatory role for CTRP3 in bone regeneration, we phenotyped skeletal development and fracture healing in CTRP3 knockout (KO) and CTRP3 overexpressing transgenic (TG) mice relative to wild-type (WT) control animals...
December 6, 2019: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://read.qxmd.com/read/31665177/a-pdgfr%C3%AE-pi3k-signaling-axis-mediates-periosteal-cell-activation-during-fracture-healing
#24
JOURNAL ARTICLE
Laura Doherty, Jungeun Yu, Xi Wang, Kurt D Hankenson, Ivo Kalajzic, Archana Sanjay
Insufficient and delayed fracture healing remain significant public health problems with limited therapeutic options. Phosphoinositide 3-kinase (PI3K) signaling, a major pathway involved in regulation of fracture healing, promotes proliferation, migration, and differentiation of osteoprogenitors. We have recently reported that knock-in mice with a global increase in PI3K signaling (gCblYF) show enhanced femoral fracture healing characterized by an extraordinary periosteal response to injury. Interestingly, of all growth factor receptors involved in fracture healing, PI3K directly binds only to PDGFR...
2019: PloS One
https://read.qxmd.com/read/31650536/human-amniotic-fluid-stem-cells-attract-osteoprogenitor-cells-in-bone-healing
#25
JOURNAL ARTICLE
Mariangela Basile, Francesco Marchegiani, Sanja Novak, Ivo Kalajzic, Roberta Di Pietro
Current treatments of large bone defects are based on autologous or allogenic bone transplantation. Human amniotic fluid stem cells (hAFSCs) were evaluated for their potential in bone regenerative medicine. In this study, hAFSCs were transduced with lentiviral vector harboring red fluorescent protein to investigate their role in the regeneration of critical-size bone defects in calvarial mouse model. To distinguish donor versus recipient cells, a transgenic mouse model carrying GFP fluorescent reporter was used as recipient to follow the fate of hAFSCs transplanted in vivo into Healos® scaffold...
October 24, 2019: Journal of Cellular Physiology
https://read.qxmd.com/read/31173397/a-new-osteocytic-cell-line-raising-new-questions-and-opportunities
#26
EDITORIAL
Ivo Kalajzic
No abstract text is available yet for this article.
June 7, 2019: Journal of Bone and Mineral Research
https://read.qxmd.com/read/31131345/pdgf-modulates-bmp2-induced-osteogenesis-in-periosteal-progenitor-cells
#27
JOURNAL ARTICLE
Xi Wang, Brya G Matthews, Jungeun Yu, Sanja Novak, Danka Grcevic, Archana Sanjay, Ivo Kalajzic
BMPs are used in various clinical applications to promote bone formation. The limited success of the BMPs in clinical settings and supraphysiological doses required for their effects prompted us to evaluate the influence of other signaling molecules, specifically platelet-derived growth factor (PDGF) on BMP2-induced osteogenesis. Periosteal cells make a major contribution to fracture healing. We detected broad expression of PDGF receptor beta (PDGFRβ) within the intact periosteum and healing callus during fracture repair...
May 2019: JBMR Plus
https://read.qxmd.com/read/30658124/aligned-microchannel-polymer-nanotube-composites-for-peripheral-nerve-regeneration-small-molecule-drug-delivery
#28
JOURNAL ARTICLE
Ohan S Manoukian, Michael R Arul, Swetha Rudraiah, Ivo Kalajzic, Sangamesh G Kumbar
Peripheral nerve injury accounts for roughly 2.8% of all trauma patients with an annual cost of 7 billion USD in the U.S. alone. Current treatment options rely on surgical intervention with the use of an autograft, despite associated shortcomings. Engineered nerve guidance conduits, stem cell therapies, and transient electrical stimulation have reported to increase speeds of functional recovery. As an alternative to the conduction effects of electrical stimulation, we have designed and optimized a nerve guidance conduit with aligned microchannels for the sustained release of a small molecule drug that promotes nerve impulse conduction...
February 28, 2019: Journal of Controlled Release
https://read.qxmd.com/read/30522780/skeletal-phenotype-of-the-neuropeptide-y-knockout-mouse
#29
JOURNAL ARTICLE
Natalie K Y Wee, Benjamin P Sinder, Sanja Novak, Xi Wang, Chris Stoddard, Brya G Matthews, Ivo Kalajzic
Neuropeptide Y (NPY) is involved in multiple processes such as behavior, energy and bone metabolism. Previous studies have relied on global NPY depletion to examine its effects on bone. However, this approach is unable to distinguish the central or local source of NPY influencing bone. Our aim was to identify which cells within the skeleton express Npy and establish a model that will enable us to differentiate effects of NPY derived from different cell types. We have generated the NPY floxed (NPYflox ) mice using CRISPR technology...
November 30, 2018: Neuropeptides
https://read.qxmd.com/read/29929058/responses-to-spaceflight-of-mouse-mandibular-bone-and-teeth
#30
JOURNAL ARTICLE
Didem Dagdeviren, Zana Kalajzic, Douglas J Adams, Ivo Kalajzic, Alan Lurie, Maija I Mednieks, Arthur R Hand
OBJECTIVE: To determine if spaceflight and microgravity affect non-weight bearing bones and development and mineralization of teeth, reasoning that combining an organ and a cellular level approach can lead to greater insights about these effects. DESIGN: Mandibles and incisors of mice flown on the US STS-135 space shuttle mission and the Russian Bion-M1 satellite were studied using micro-computed tomography and immunohistochemistry. Ground controls were mice housed in standard vivarium cages and flight habitats...
June 7, 2018: Archives of Oral Biology
https://read.qxmd.com/read/29556234/the-long-pentraxin-3-plays-a-role-in-bone-turnover-and-repair
#31
JOURNAL ARTICLE
Danka Grčević, Marina Sironi, Sonia Valentino, Livija Deban, Hrvoje Cvija, Antonio Inforzato, Nataša Kovačić, Vedran Katavić, Tomislav Kelava, Ivo Kalajzić, Alberto Mantovani, Barbara Bottazzi
Pentraxin 3 (PTX3) is an inflammatory mediator acting as a fluid-phase pattern recognition molecule and playing an essential role in innate immunity and matrix remodeling. Inflammatory mediators also contribute to skeletal homeostasis, operating at multiple levels in physiological and pathological conditions. This study was designed to investigate the role of PTX3 in physiological skeletal remodeling and bone healing. Micro-computed tomography (μCT) and bone histomorphometry of distal femur showed that PTX3 gene-targeted female and male mice ( ptx3-/- ) had lower trabecular bone volume than their wild-type ( ptx3+/+ ) littermates (BV/TV by μCT: 3...
2018: Frontiers in Immunology
https://read.qxmd.com/read/29178402/micro-nanostructures-of-cellulose-collagen-for-critical-sized-bone-defect-healing
#32
JOURNAL ARTICLE
Aja Aravamudhan, Daisy M Ramos, Jonathan Nip, Ivo Kalajzic, Sangamesh G Kumbar
Bone tissue engineering strategies utilize biodegradable polymeric matrices alone or in combination with cells and factors to provide mechanical support to bone, while promoting cell proliferation, differentiation, and tissue ingrowth. The performance of mechanically competent, micro-nanostructured polymeric matrices, in combination with bone marrow stromal cells (BMSCs), is evaluated in a critical sized bone defect. Cellulose acetate (CA) is used to fabricate a porous microstructured matrix. Type I collagen is then allowed to self-assemble on these microstructures to create a natural polymer-based, micro-nanostructured matrix (CAc)...
February 2018: Macromolecular Bioscience
https://read.qxmd.com/read/28600151/splenomegaly-myeloid-lineage-expansion-and-increased-osteoclastogenesis-in-osteogenesis-imperfecta-murine
#33
JOURNAL ARTICLE
Brya G Matthews, Emilie Roeder, Xi Wang, Hector Leonardo Aguila, Sun-Kyeong Lee, Danka Grcevic, Ivo Kalajzic
Osteogenesis imperfecta (OI) is a disease caused by defects in type I collagen production that results in brittle bones. While the pathology is mainly caused by defects in the osteoblast lineage, there is also elevated bone resorption by osteoclasts resulting in high bone turnover in severe forms of the disease. Osteoclasts originate from hematopoietic myeloid cells, however changes in hematopoiesis have not been previously documented in OI. In this study, we evaluated hematopoietic lineage distribution and osteoclast progenitor cell frequency in bone marrow, spleen and peripheral blood of osteogenesis imperfecta murine (OIM) mice, a model of severe OI...
October 2017: Bone
https://read.qxmd.com/read/28297566/instructive-role-of-the-microenvironment-in-preventing-renal-fibrosis
#34
JOURNAL ARTICLE
Kei Matsumoto, Sandhya Xavier, Jun Chen, Yujiro Kida, Mark Lipphardt, Reina Ikeda, Annie Gevertz, Mario Caviris, Antonis K Hatzopoulos, Ivo Kalajzic, James Dutton, Brian B Ratliff, Hong Zhao, Zbygniew Darzynkiewicz, Stefan Rose-John, Michael S Goligorsky
Accumulation of myofibroblasts is a hallmark of renal fibrosis. A significant proportion of myofibroblasts has been reported to originate via endothelial-mesenchymal transition. We initially hypothesized that exposing myofibroblasts to the extract of endothelial progenitor cells (EPCs) could reverse this transition. Indeed, in vitro treatment of transforming growth factor-β1 (TGF-β1)-activated fibroblasts with EPC extract prevented expression of α-smooth muscle actin (α-SMA); however, it did not enhance expression of endothelial markers...
March 2017: Stem Cells Translational Medicine
https://read.qxmd.com/read/27721375/exploiting-endogenous-fibrocartilage-stem-cells-to-regenerate-cartilage-and-repair-joint-injury
#35
JOURNAL ARTICLE
Mildred C Embree, Mo Chen, Serhiy Pylawka, Danielle Kong, George M Iwaoka, Ivo Kalajzic, Hai Yao, Chancheng Shi, Dongming Sun, Tzong-Jen Sheu, David A Koslovsky, Alia Koch, Jeremy J Mao
Tissue regeneration using stem cell-based transplantation faces many hurdles. Alternatively, therapeutically exploiting endogenous stem cells to regenerate injured or diseased tissue may circumvent these challenges. Here we show resident fibrocartilage stem cells (FCSCs) can be used to regenerate and repair cartilage. We identify FCSCs residing within the superficial zone niche in the temporomandibular joint (TMJ) condyle. A single FCSC spontaneously generates a cartilage anlage, remodels into bone and organizes a haematopoietic microenvironment...
October 10, 2016: Nature Communications
https://read.qxmd.com/read/27708132/instructive-role-of-the-microenvironment-in-preventing-renal-fibrosis
#36
JOURNAL ARTICLE
Kei Matsumoto, Sandhya Xavier, Jun Chen, Yujiro Kida, Mark Lipphardt, Reina Ikeda, Annie Gevertz, Mario Caviris, Antonis K Hatzopoulos, Ivo Kalajzic, James Dutton, Brian B Ratliff, Hong Zhao, Zbygniew Darzynkiewicz, Stefan Rose-John, Michael S Goligorsky
: Accumulation of myofibroblasts is a hallmark of renal fibrosis. A significant proportion of myofibroblasts has been reported to originate via endothelial-mesenchymal transition. We initially hypothesized that exposing myofibroblasts to the extract of endothelial progenitor cells (EPCs) could reverse this transition. Indeed, in vitro treatment of transforming growth factor-β1 (TGF-β1)-activated fibroblasts with EPC extract prevented expression of α-smooth muscle actin (α-SMA); however, it did not enhance expression of endothelial markers...
October 5, 2016: Stem Cells Translational Medicine
https://read.qxmd.com/read/27616604/visual-reporters-for-study-of-the-osteoblast-lineage
#37
REVIEW
Emilie Roeder, Brya G Matthews, Ivo Kalajzic
Advancing our understanding of osteoblast biology and differentiation is critical to elucidate the pathological mechanisms responsible for skeletal diseases such as osteoporosis. Histology and histomorphometry, the classical methods to study osteoblast biology, identify osteoblasts based on their location and morphology and ability to mineralize matrix, but do not clearly define their stage of differentiation. Introduction of visual transgenes into the cells of osteoblast lineage has revolutionized the field and resulted in a paradigm shift that allowed for specific identification and isolation of subpopulations within the osteoblast lineage...
November 2016: Bone
https://read.qxmd.com/read/27612450/increased-chemotaxis-and-activity-of-circulatory-myeloid-progenitor-cells-may-contribute-to-enhanced-osteoclastogenesis-and-bone-loss-in-the-c57bl-6-mouse-model-of-collagen-induced-arthritis
#38
JOURNAL ARTICLE
M Ikić Matijašević, D Flegar, N Kovačić, V Katavić, T Kelava, A Šućur, S Ivčević, H Cvija, E Lazić Mosler, I Kalajzić, A Marušić, D Grčević
Our study aimed to determine the functional activity of different osteoclast progenitor (OCP) subpopulations and signals important for their migration to bone lesions, causing local and systemic bone resorption during the course of collagen-induced arthritis in C57BL/6 mice. Arthritis was induced with chicken type II collagen (CII), and assessed by clinical scoring and detection of anti-CII antibodies. We observed decreased trabecular bone volume of axial and appendicular skeleton by histomorphometry and micro-computed tomography as well as decreased bone formation and increased bone resorption rate in arthritic mice in vivo...
December 2016: Clinical and Experimental Immunology
https://read.qxmd.com/read/27507737/quiescent-bone-lining-cells-are-a-major-source-of-osteoblasts-during-adulthood
#39
JOURNAL ARTICLE
Igor Matic, Brya G Matthews, Xi Wang, Nathaniel A Dyment, Daniel L Worthley, David W Rowe, Danka Grcevic, Ivo Kalajzic
The in vivo origin of bone-producing osteoblasts is not fully defined. Skeletal stem cells, a population of mesenchymal stem cells resident in the bone marrow compartment, are thought to act as osteoprogenitors during growth and adulthood. Quiescent bone lining cells (BLCs) have been suggested as a population capable of activation into mature osteoblasts. These cells were defined by location and their morphology and studies addressing their significance have been hampered by their inaccessibility, and lack of markers that would allow for their identification and tracing...
December 2016: Stem Cells
https://read.qxmd.com/read/27184388/murine-supraspinatus-tendon-injury-model-to-identify-the-cellular-origins-of-rotator-cuff-healing
#40
JOURNAL ARTICLE
Ryu Yoshida, Farhang Alaee, Felix Dyrna, Mark S Kronenberg, Peter Maye, Ivo Kalajzic, David W Rowe, Augustus D Mazzocca, Nathaniel A Dyment
Purpose of this study: To elucidate the origin of cell populations that contribute to rotator cuff healing, we developed a mouse surgical model where a full-thickness, central detachment is created in the supraspinatus. MATERIALS AND METHODS: Three different inducible Cre transgenic mice with Ai9-tdTomato reporter expression (PRG4-9, αSMA-9, and AGC-9) were used to label different cell populations in the shoulder. The defect was created surgically in the supraspinatus. The mice were injected with tamoxifen at surgery to label the cells and sacrificed at 1, 2, and 5 weeks postoperatively...
November 2016: Connective Tissue Research
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