keyword
MENU ▼
Read by QxMD icon Read
search

cancer cell culture

keyword
https://www.readbyqxmd.com/read/28944451/expression-of-mirna-26b-5p-and-its-target-trps1-is-associated-with-radiation-exposure-in-post-chernobyl-breast-cancer
#1
Christina M Wilke, Julia Hess, Sergiy V Klymenko, Vadim V Chumak, Liubov M Zakhartseva, Elena V Bakhanova, Annette Feuchtinger, Axel K Walch, Martin Selmansberger, Herbert Braselmann, Ludmila Schneider, Adriana Pitea, Julia Steinhilber, Falko Fend, Hans C Bösmüller, Horst Zitzelsberger, Kristian Unger
Ionising radiation is a well-recognised risk factor for the development of breast cancer, however, it is unknown whether radiation-specific molecular oncogenic mechanisms exist. We investigated post-Chernobyl breast cancers from radiation-exposed female clean-up workers and non-exposed controls for molecular changes. Radiation-associated alterations identified in the discovery cohort (n=38) were subsequently validated in a second cohort (n=39). Increased expression of hsa-miR-26b-5p was associated with radiation exposure in both of the cohorts...
September 25, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28944383/-open-top-microfluidic-device-for-in-vitro-three-dimensional-capillary-beds
#2
Soojung Oh, Hyunryul Ryu, Dongha Tahk, Jihoon Ko, Yoojin Chung, Hae Kwang Lee, Tae Ryong Lee, Noo Li Jeon
We introduce a novel microfluidic device to co-culture a blood vessel network and cell tissues in an in vivo-like niche. Our "open-top" microfluidic device is composed of microchannels with micropores in the ceiling, which provides direct fluid access from reservoir to microchannel. Fluid connections through micropores afford novel advantages, including: i) the long-term culture of large-scale microvessel network, ii) access of different fluids to inner and exterior sides of the microvessel, and iii) co-culturing of the microvessel network and small cell tissue...
September 25, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28943963/expression-of-the-tumor-suppressor-gene-p16-and-lymph-node-metastasis-in-patients-with-ovarian-cancer
#3
Hongyan Wang, Jingfang Zheng, Qiang Li, Min Zhou, Dongmei Ai, Hui Zhang
This study examined the relationship between the expression of p16, a tumor suppressor gene, and lymph node metastasis, as well as patient prognosis, in cases with ovarian cancer. SKOV-3, an ovarian cancer cell line, and IOSE80, a normal human ovarian cell line, were selected for testing. Western blot analysis was used to detect the p16 expression in ovarian cell culture samples. In the study, 20 cases with normal ovarian tissue and 64 cases with ovarian cancer tissue, including 38 cases with lymph node metastasis and 26 cases without lymph node metastasis, were also selected for testing...
October 2017: Oncology Letters
https://www.readbyqxmd.com/read/28943917/mechanisms-of-cxcr7-induction-in-malignant-melanoma-development
#4
Xiao-Jing Li, Pai Liu, Wei-Wei Tian, Zhi-Feng Li, Bao-Guo Liu, Jian-Fang Sun
Malignant melanoma (MM) is a highly malignant skin tumor. The mechanism of MM pathogenesis and its signaling pathways are not well characterized. C-X-C chemokine receptor type 7 (CXCR7) has been reported to regulate cancer cell invasion. The present study sought to investigate the effects of CXCR7 on MM development. First, CXCR7 expression levels were assessed in the skin tumor tissue of patients with MM. Then, CXCR7 small hairpin RNA was used in M14 melanoma cells in a Transwell culture model and in a transplanted mouse model to test the effects of CXCR7...
October 2017: Oncology Letters
https://www.readbyqxmd.com/read/28943913/relationship-between-the-expression-of-cd133-hif-1%C3%AE-vegf-and-the-proliferation-and-apoptosis-in-hypoxic-human-prostate-cancer-cells
#5
Lingyun Liu, Zuowen Liang, Kaimin Guo, Hongliang Wang
This study measured the levels of expression of CD133, hypoxia-inducible factor (HIF)-1α and vascular endothelial growth factor (VEGF) in human prostate cancer cells grown under hypoxic and non-hypoxic conditions to compare the values to resulting amounts of proliferation and apoptosis in the cells. Human prostate cancer cell line LNCaP cells were routinely thawed, cultured and passaged. Actively growing cells were divided into batches. Cells in the control group were grown under 5% CO2 + 20% O2, and those in the hypoxia group were grown under 5% CO2 + 1% O2...
October 2017: Oncology Letters
https://www.readbyqxmd.com/read/28942682/near-infrared-light-mediated-photochemotherapy-for-efficiently-treating-deep-orthotopic-tumors-guided-by-ultrasound-imaging
#6
Zuhua Wang, Shaoyan Xuan, Wenqi Qiu, Jiang Zhu, Xiaomeng Guo, Wei Li, Hanbo Zhang, Xiuliang Zhu, Yong-Zhong Du, Jian You
Recently, Combined cancer photothermal-chemotherapy has become a highly promising strategy in cancer treatment for its enhanced therapeutic efficacy, controlled drug release and reduced systemic toxicity. Almost all the reported strategies based on photothermal-chemotherapy have only focused on the treatment of superficial or subcutaneous cancer, which are not considered as a more clinically relevant and better predictive models of drug efficacy than orthotopic tumor models. Here, we reported an EphB4 receptor-targeting polymeric nanoplatform containing hollow gold nanospheres (HAuNS) and the anticancer drug paclitaxel (PTX) for cancer photothermal-chemotherapy...
November 2017: Drug Delivery
https://www.readbyqxmd.com/read/28942439/functional-consequences-of-intracellular-proline-levels-manipulation-affecting-prodh-pox-dependent-pro-apoptotic-pathways-in-a-novel-in-vitro-cell-culture-model
#7
Ilona Zareba, Arkadiusz Surazynski, Marcin Chrusciel, Wojciech Miltyk, Milena Doroszko, Nafis Rahman, Jerzy Palka
BACKGROUND/AIMS: The effect of impaired intracellular proline availability for proline dehydrogenase/proline oxidase (PRODH/POX)-dependent apoptosis was studied. METHODS: We generated a constitutively knocked-down PRODH/POX MCF-7 breast cancer cell line (MCF-7shPRODH/POX) as a model to analyze the functional consequences of impaired intracellular proline levels. We have used inhibitor of proline utilization in collagen biosynthesis, 2-metoxyestradiol (MOE), inhibitor of prolidase that generate proline, rapamycin (Rap) and glycyl-proline (GlyPro), substrate for prolidase...
September 22, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28941302/shh-gene-silencing-suppresses-epithelial-mesenchymal-transition-proliferation-invasion-and-migration-of-cervical-cancer-cells-by-repressing-the-hedgehog-signaling-pathway
#8
Feng Zhang, Chen-Chen Ren, Ling Liu, Yan-Nan Chen, Li Yang, Xiao-An Zhang, Xiao-Ming Wang, Feng-Jing Yu
The study aimed to investigate the mechanism by which the sonic Hedgehog (SHH) gene silencing acts upon epithelial-mesenchymal transition (EMT), proliferation, invasion and migration of cervical cancer (CC) cells via the Hedgehog signaling pathway. RT-qPCR and western blotting were all employed to detect the SHH mRNA and protein expressions. HeLa and CasKi cells were cultured and subsequently divided into the blank, negative control (NC) and SHH-RNAi groups. A cell counting kit-8 (CCK-8) assay was utilized for cell proliferation...
September 23, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28941016/ethyl-acetate-fraction-from-methanol-extraction-of-vitis-thunbergii-var-taiwaniana-induced-g0-g1-phase-arrest-via-inhibition-of-cyclins-d-and-e-and-induction-of-apoptosis-through-caspase-dependent-and-independent-pathways-in-human-prostate-carcinoma-du145-cells
#9
Chia-Hsin Lin, Hsiao-Sung Chan, Hsin-Sheng Tsay, Shinji Funayama, Chao-Lin Kuo, Jing-Gung Chung
Vitis thunbergii var. taiwaniana (VTT) is a wild grape native to Taiwan, belonging to the Vitaceae family and Vitis genus, and widely used as folk herbal medicine. It is traditionally used for the treatment of diarrhea, hypertension, neuroprotection, jaundice, and arthritis. We used the wild-collected VTT and sterilized them to establish the plant tissue culture, and then took the leaves for DNA sequencing to determine its original base. We use methanol to extract VTT in four different solvents: 1-butanol, n-hexane, ethyl acetate, and water...
September 22, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/28940860/nanoparticles-prepared-from-porcine-cells-support-the-healing-of-cutaneous-inflammation-in-mice-and-wound-re-epithelialization-in-human-skin
#10
Natalia Kunz, Eva Hauenschild, Sebastian Maass, Kai-Uwe Kalies, Matthias Klinger, Melanie Barra, Lars Hecht, Franz Helbig, Stefan Soellner, Charles C Caldwell, Ralf J Ludwig, Jürgen Westermann, Kathrin Kalies
Previous reports have demonstrated that cell-derived nanoparticles (CDNPs) composed of bovine or porcine protein complexes exerted therapeutic effects against viral infections and cancer in mice and humans. Based on these observations, we asked whether CDNPs would improve inflammatory skin disorders. To address this, we utilized two distinct mouse models of cutaneous inflammation: the autoimmune skin blistering disease epidermolysis bullosa acquisita (EBA) as an example of an autoantibody-induced cutaneous inflammation, and Leishmania major (L...
September 23, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28940499/substrate-stiffness-regulated-migration-and-angiogenesis-potential-of-a549-cells-and-huvecs
#11
Dan Zhao, Changyue Xue, Qianshun Li, Mengting Liu, Wenjuan Ma, Tengfei Zhou, Yunfeng Lin
Tumor tissue tends to stiffen during solid tumor progression. Substrate stiffness is known to alter cell behaviors, such as proliferation and migration, during which angiogenesis is requisite. Mono-culture and co-culture systems of lung cancer cell line A549 and human umbilical vein endothelial cells (HUVECs), on polydimethylsiloxane substrates (PDMS) with varying stiffness, were used for investigating the effects of substrate stiffness on the migration and angiogenesis of lung cancer. The expressions of matrix metalloproteinases (MMPs) and angiogenesis-related growth factors were up-regulated with the increase of substrate stiffness, whereas that of tissue inhibitor of matrix metalloproteinase (TIMPs) were down-regulated with increasing substrate stiffness...
September 21, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28940439/detection-of-abcb5-tumour-antigen-specific-cd8-t-cells-in-melanoma-patients-and-implications-for-immunotherapy
#12
Sylvia Borchers, Christoph Masslo, Christina Ann Müller, Anika Tahedl, Jennifer Volkind, Yvonne Nowak, Viktor Umansky, Jasmina Esterlechner, Markus Hermann Frank, Christoph Ganss, Mark Andreas Kluth, Jochen Utikal
ABCB5 has been identified as a tumour initiating cell marker and is expressed in various malignancies, including melanoma. Moreover, treatment with anti-ABCB5 monoclonal antibodies has been shown to inhibit tumour growth in xenotransplantation models. Therefore, ABCB5 represents a potential target for cancer immunotherapy. However, cellular immune responses against ABCB5 in humans have not been described so far. Here, we investigated whether ABCB5-reactive T cells are present in human melanoma patients and tested the applicability of ABCB5-derived peptides for experimental induction of human T cell responses...
September 23, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28940260/authentication-of-m14-melanoma-cell-line-proves-misidentification-of-mda-mb-435-breast-cancer-cell-line
#13
Christopher Korch, Erin M Hall, Wilhelm G Dirks, Margaret Ewing, Mark Faries, Marileila Varella-Garcia, Steven Robinson, Douglas Storts, Jacqueline A Turner, Ying Wang, Edward C Burnett, Lyn Healy, Douglas Kniss, Richard M Neve, Raymond W Nims, Yvonne A Reid, William A Robinson, Amanda Capes-Davis
A variety of analytical approaches have indicated that melanoma cell line UCLA-SO-M14 (M14) and breast carcinoma cell line MDA-MB-435 originate from a common donor. This indicates that at some point in the past, one of these cell lines became misidentified, meaning that it ceased to correspond to the reported donor and instead became falsely identified (through cross-contamination or other means) as a cell line from a different donor. Initial studies concluded that MDA-MB-435 was the misidentified cell line and M14 was the authentic cell line, although contradictory evidence has been published, resulting in further confusion...
September 22, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28940194/a-new-model-system-identifies-egfr-her2-and-her2-her3-heterodimers-as-potent-inducers-of-oesophageal-epithelial-cell-invasion
#14
Christiane Daniela Fichter, Camilla Maria Przypadlo, Achim Buck, Nicola Herbener, Bianca Riedel, Luisa Schäfer, Hiroshi Nakagawa, Axel Walch, Thomas Reinheckel, Martin Werner, Silke Lassmann
Oesophageal squamous cell carcinomas and oesophageal adenocarcinomas display distinct patterns of ErbB expression and dimers. The functional effects of specific ErbB homo- or heterodimers on oesophageal (cancer) cell behaviour, particularly invasion of early carcinogenesis remains unknown. Here, a new cellular model system for controlled activation of EGFR or HER2 and EGFR/HER2 or HER2/HER3 homo- and heterodimers was studied in non-neoplastic squamous oesophageal epithelial Het-1A cells. EGFR, HER2 and HER3 intracellular domains (ICDs) were fused to dimerization domains (DmrA / DmrA and DmrC), and transduced into Het-1A cells lacking ErbB expression...
September 20, 2017: Journal of Pathology
https://www.readbyqxmd.com/read/28939462/enhanced-cell-viability-in-hyaluronic-acid-coated-poly-lactic-co-glycolic-acid-porous-scaffolds-within-microfluidic-channels
#15
Fernanda Zamboni, Marie Keays, Sheri Hayes, Ahmad B Albadarin, Gavin M Walker, Patrick A Kiely, Maurice N Collins
The concept of the present work is to produce porous optimised scaffolds of poly(lactic-co-glycolic acid) (PLGA) coated with hyaluronic acid (HA), to provide a suitable microenvironment for cellular proliferation. Freeze dried scaffolds were produced from PLGA with varying lactic acid and glycolic acid ratios along the polymer backbone, as follows: 50:50 ester terminated, 50:50 carboxylate end-group and 85:15 ester terminated. Subsequently, these scaffolds were immersed in crosslinked HA in order for the coating to enhance biological performance...
September 19, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28939108/differential-response-to-doxorubicin-in-breast-cancer-subtypes-simulated-by-a-microfluidic-tumor-model
#16
Altug Ozcelikkale, Kyeonggon Shin, Victoria Noe-Kim, Bennett D Elzey, Zizheng Dong, Jian-Ting Zhang, Kwangmeyung Kim, Ick Chan Kwon, Kinam Park, Bumsoo Han
Successful drug delivery and overcoming drug resistance are the primary clinical challenges for management and treatment of cancer. The ability to rapidly screen drugs and delivery systems within physiologically relevant environments is critically important; yet is currently limited due to lack of appropriate tumor models. To address this problem, we developed the Tumor-microenvironment-on-chip (T-MOC), a new microfluidic tumor model simulating the interstitial flow, plasma clearance, and transport of the drug within the tumor...
September 19, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28938744/an-improved-ivm-method-for-cumulus-oocyte-complexes-from-small-follicles-in-polycystic-ovary-syndrome-patients-enhances-oocyte-competence-and-embryo-yield
#17
F Sánchez, F Lolicato, S Romero, M De Vos, H Van Ranst, G Verheyen, E Anckaert, J E J Smitz
STUDY QUESTION: Are meiotic and developmental competence of human oocytes from small (2-8 mm) antral follicles improved by applying an optimized IVM method involving a prematuration step in presence of C-Type Natriuretic Peptide (CNP) followed by a maturation step in presence of FSH and Amphiregulin (AREG)? SUMMARY ANSWER: A strategy involving prematuration culture (PMC) in the presence of CNP followed by IVM using FSH + AREG increases oocyte maturation potential leading to a higher availability of Day 3 embryos and good-quality blastocysts for single embryo transfer...
October 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28938696/the-molecular-mechanisms-of-chemoresistance-in-cancers
#18
REVIEW
Hua-Chuan Zheng
Overcoming intrinsic and acquired drug resistance is a major challenge in treating cancer patients because chemoresistance causes recurrence, cancer dissemination and death. This review summarizes numerous molecular aspects of multi-resistance, including transporter pumps, oncogenes (EGFR, PI3K/Akt, Erk and NF-κB), tumor suppressor gene (p53), mitochondrial alteration, DNA repair, autophagy, epithelial-mesenchymal transition (EMT), cancer stemness, and exosome. The chemoresistance-related proteins are localized to extracellular ligand, membrane receptor, cytosolic signal messenger, and nuclear transcription factors for various events, including proliferation, apoptosis, EMT, autophagy and exosome...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938555/exosomes-of-invasive-urothelial-carcinoma-cells-are-characterized-by-a-specific-mirna-expression-signature
#19
Sophie Baumgart, Sebastian Hölters, Carsten-Henning Ohlmann, Rainer Bohle, Michael Stöckle, Marie Stampe Ostenfeld, Lars Dyrskjøt, Kerstin Junker, Joana Heinzelmann
Muscle-invasive bladder cancer (MIBC) represents a highly aggressive tumor type compared to non-muscle-invasive tumors. MIBC is characterized by specific molecular alterations, which may also modulate extracellular tumorigenic effects. Tumor-associated exosomes, especially exosomal miRNAs, are important regulators in the interaction between tumor cells and tumor microenvironment by affecting tumor-promoting processes in target cells. It is important to analyze whether their exosomal patterns also reflect the specific molecular characteristics of MIBC...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938000/podoplanin-increases-the-migration-of-human-fibroblasts-and-affects-the-endothelial-cell-network-formation-a-possible-role-for-cancer-associated-fibroblasts-in-breast-cancer-progression
#20
Jaroslaw Suchanski, Anna Tejchman, Maciej Zacharski, Aleksandra Piotrowska, Jedrzej Grzegrzolka, Grzegorz Chodaczek, Katarzyna Nowinska, Janusz Rys, Piotr Dziegiel, Claudine Kieda, Maciej Ugorski
In our previous studies we showed that in breast cancer podoplanin-positive cancer-associated fibroblasts correlated positively with tumor size, grade of malignancy, lymph node metastasis, lymphovascular invasion and poor patients' outcome. Therefore, the present study was undertaken to assess if podoplanin expressed by fibroblasts can affect malignancy-associated properties of breast cancer cells. Human fibroblastic cell lines (MSU1.1 and Hs 578Bst) overexpressing podoplanin and control fibroblasts were co-cultured with breast cancer MDA-MB-231 and MCF7 cells and the impact of podoplanin expressed by fibroblasts on migration and invasiveness of breast cancer cells were studied in vitro...
2017: PloS One
keyword
keyword
84439
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"