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https://www.readbyqxmd.com/read/28413519/amp-activated-protein-kinase-mediates-the-effects-of-lipoprotein-associated-phospholipase-a2-on-endothelial-dysfunction-in-atherosclerosis
#1
Li Yang, Hong-Liang Cong, Shu-Feng Wang, Ting Liu
The present study aimed to investigate the effects of lipoprotein-associated phospholipase A2 (Lp-PLA2) on endothelial dysfunction in an in vitro cell model of atherosclerosis, and to determine whether AMP-activated protein kinase (AMPK) mediates the effects of Lp-PLA2 on endothelial dysfunction. A total of 392 patients with coronary artery disease (CAD), including various sub-conditions, were recruited, and the plasma concentrations of Lp-PLA2 were evaluated. In addition, an in vitro model of atherosclerosis was established by exposing human umbilical vein endothelial cells (HUVECs) to oxidized low-density lipoprotein (oxLDL)...
April 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28377266/the-lipid-moiety-7-ketocholesteryl-9-carboxynonanoate-mediates-binding-interaction-of-oxldl-to-lox-1-and-upregulates-abca1-expression-through-ppar%C3%AE
#2
Jingda Li, Zhilong Xiu, Renjun Wang, Chengjie Yu, Yan Chi, Jianzhong Qin, Changzhen Fu, Eiji Matsuura, Qingping Liu
AIM: Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1), a specific membrane receptor for oxidized low-density lipoprotein (oxLDL), plays a crucial role in atherosclerosis progression. The aim of this study was to elucidate the role of 7-ketocholesteryl-9-carboxynonanoate (oxLig-1), a lipid component of oxLDL, in the binding of oxLDL to LOX-1 and to determine whether oxLig-1 binding to LOX-1 is involved in the upregulation of ABCA1 expression. MAIN METHODS: OxLig-1 binding to LOX-1 was analysed by AutoDock 4...
April 1, 2017: Life Sciences
https://www.readbyqxmd.com/read/28334721/microrna-9-inhibits-nlrp3-inflammasome-activation-in-human-atherosclerosis-inflammation-cell-models-through-the-jak1-stat-signaling-pathway
#3
Yue Wang, Zhihua Han, Yuqi Fan, Junfeng Zhang, Kan Chen, Lin Gao, Huasu Zeng, Jiatian Cao, Changqian Wang
BACKGROUND/AIMS: MicroRNA-9 (miR-9) is involved in inflammatory reaction in atherosclerosis; however, its function and regulatory mechanisms remain unclear. We aimed to uncover the exact roles of miR-9 and downstream signaling pathways using in vitro human atherosclerosis models. METHODS: We used oxidized low-density lipoprotein (oxLDL)-stimulated human THP-1 derived macrophages, oxLDL-stimulated human primary peripheral blood monocytes and lipopolysaccharides (LPS) or Alum-stimulated human THP-1 derived macrophages as in vitro atherosclerosis inflammation models...
March 27, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28304296/pcsk9-promotes-oxldl-induced-pc12-cell-apoptosis-through-the-bcl-2-bax-caspase-9-3-signaling-pathway
#4
Lu-Shan Liu, Xue-Qin Bai, Ya Gao, Qi Wu, Zhong Ren, Qing Li, Li-Hong Pan, Ni-Ya He, Juan Peng, Zhi-Han Tang
BACKGROUND: Hyperlipidemia is a risk factor for neurodegenerative diseases. Proprotein convertase subtilisin / Kexin type 9 (PCSK9) degrades hepatic low-density lipoprotein receptor (LDLR) to regulate lipid metabolism. It is unclear if PCSK9 plays a role in neurodegenerative diseases. OBJECTIVE: This study was designed to determine whether PCSK9 is crucial between hyperlipidemia and Alzheimer's disease. The interrelationship between PCSK9 and neuronal apoptosis was explored in PC12 cells in response to treatment with oxidized low-density lipoprotein (oxLDL)...
March 10, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28089094/postprandial-clearance-of-oxidized-low-density-lipoprotein-in-patients-with-stroke-due-to-atherosclerosis
#5
Sami Al Kasab, Christy Cassarly, Ngoc-Anh Le, Renee Martin, Julia Brinley, Marc I Chimowitz, Tanya N Turan
BACKGROUND: Oxidized low-density lipoprotein (OxLDL) is a contributor to atherosclerosis development. OxLDL formation increases in the postprandial state due to oxidative stress in subjects with coronary artery disease (CAD) and diabetes, but has not been studied in patients with atherosclerotic stroke. We aimed to determine differences in postprandial OxLDL in patients with atherosclerotic stroke compared to stroke from other causes. METHODS: Patients with ischemic stroke but no history of CAD (n = 42) were enrolled and categorized by stroke subtype as extracranial atherosclerosis (EC), n = 12; intracranial atherosclerosis (IC), n = 16; or other cause, n = 14...
January 6, 2017: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
https://www.readbyqxmd.com/read/27956218/an-intensive-lifestyle-intervention-reduces-circulating-oxidised-low-density-lipoprotein-and-increases-human-paraoxonase-activity-in-obese-subjects
#6
Angelo Russo, Irene Pirisinu, Carmine Vacca, Elisa Reginato, Emilia Sbroma Tomaro, Roberto Pippi, Cristina Aiello, Vincenzo Nicola Talesa, Pierpaolo De Feo, Rita Romani
OBJECTIVE: Obesity has a great impact on cardiovascular morbidity and mortality, the treatment of this pathological state is important given the significant health consequences. Lifestyle and behaviour changes play a significant role in weight management. The purpose of this study was to investigate the impact of an intensive multidisciplinary lifestyle intervention on well-known atherogenic factors in a group of overweight and obese subjects. METHODS: A total of 44 people with overweight/obesity underwent a lifestyle intervention based on nutritional education, psychological support and a 3-month exercise training program with a frequency of twice a week...
December 9, 2016: Obesity Research & Clinical Practice
https://www.readbyqxmd.com/read/27940038/increased-levels-of-legumain-in-plasma-and-plaques-from-patients-with-carotid-atherosclerosis
#7
Ngoc Nguyen Lunde, Sverre Holm, Tuva B Dahl, Inass Elyouncha, Bjørnar Sporsheim, Ida Gregersen, Azhar Abbas, Mona Skjelland, Terje Espevik, Rigmor Solberg, Harald Thidemann Johansen, Bente Halvorsen
BACKGROUND AND AIMS: The cysteine protease legumain has been shown to be up-regulated in unstable atherosclerotic plaques. This study aims to further elucidate legumain in atherosclerosis, by examining legumain in plasma and carotid plaques from patients with carotid stenosis. Furthermore, legumain secretion from monocyte-derived macrophages treated with atherogenic lipids during macrophage polarization was studied. METHODS: Plasma levels of legumain from patients with carotid stenosis (n = 254), healthy controls (n = 91), and secreted from monocyte-derived macrophages were assessed by enzyme-linked-immunosorbent assay...
February 2017: Atherosclerosis
https://www.readbyqxmd.com/read/27870764/anti-ro-and-concomitant-anti-la-autoantibodies-strongly-associated-with-anti-oxldl-or-anti-phospholipid-antibody-in-systemic-lupus-erythematosus
#8
Biji Theyilamannil Kurien, James Fesmire, Camille Jean Anderson, Robert Hal Scofield
BACKGROUND: Premature atherosclerosis is observed in systemic lupus erythematosus (SLE). Oxidative modification of LDL is associated with atherosclerotic plaque formation. OBJECTIVES: We hypothesized that anti-oxidized LDL (oxLDL) and anti-phospholipid (APL) in SLE sera would segregate with specific antibody subsets, and that anti-oxLDL antibodies will linger in circulation over an extended period. PATIENTS AND METHODS: Sixty-seven SLE and control subjects and two SLE subjects with sera collected longitudinally for 13 years were tested for anti-oxLDL and IgG/IgM/IgA APL antibodies...
December 2016: Journal of Clinical Rheumatology: Practical Reports on Rheumatic & Musculoskeletal Diseases
https://www.readbyqxmd.com/read/27825356/the-association-between-oxidized-low-density-lipoprotein-antibodies-and-hematological-diseases
#9
Hao Li, Da-Qing Li, Xiang-Xin Li, Lu-Qun Wang
BACKGROUND: The aim of the study is to compare the profiles of antibodies (IgM and IgG) against oxidized low-density lipoprotein (oxLDL) of hematological diseases. METHODS: The serum antibodies of oxLDL-IgM and oxLDL-IgG for 446 cases with hematological diseases and 90 patients with primary hypertension and 90 healthy controls were measured by enzyme-linked immunosorbent assay (ELISA) in a cross-section survey. The association of serum oxLDL-LgM and oxLDL-IgG with hematological diseases was analyzed by multiple linear regression model...
November 8, 2016: Lipids in Health and Disease
https://www.readbyqxmd.com/read/27822126/comparison-of-lipid-profile-parameters-and-oxidized-low-density-lipoprotein-between-normal-and-preeclamptic-pregnancies-in-a-tertiary-care-hospital-in-nepal
#10
Santosh Timalsina, Prajwal Gyawali, Aseem Bhattarai
INTRODUCTION: Preeclampsia is a pregnancy-specific complication that significantly contributes to maternal and perinatal morbidity and mortality worldwide, even more so in developing countries such as Nepal. The potential contribution of dyslipidemia and elevated levels of oxidized low-density lipoprotein (oxLDL) in the pathogenesis of preeclamptic pregnancies has been observed in several studies. The aim of this study was to compare the maternal lipid profile parameters and particularly oxLDL between preeclamptic and healthy pregnancies and also correlate oxLDL with other lipid profile parameters...
2016: International Journal of Women's Health
https://www.readbyqxmd.com/read/27807992/radiation-induced-surge-of-macrophage-foam-cell-formation-oxidative-damage-and-cytokine-release-is-attenuated-by-a-nanoformulation-of-curcumin
#11
Behrooz Soltani, Narges Bodaghabadi, Nasser Ghaemi, Majid Sadeghizadeh
PURPOSE: We examined the potential of a dendrosomal nanoformulation of curcumin (DNC) for intervention of ionizing radiation (IR)-induced damage (particularly leading to atherosclerosis), employing an irradiated THP-1 macrophage model. MATERIALS AND METHODS: Differentiated THP-1 macrophages were irradiated and treated with curcumin or DNC nanoformulation (and oxidized low density lipoprotein, ox-LDL, to promote foam cells). Chemical, biochemical, and genetics tools including viability and apoptosis, multiple ELISA, real-time PCR, Western blotting, enzyme activity, and fluorimetry assays were employed to illustrate IR damage as well as the DNC intervention potential...
November 3, 2016: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/27801365/oxidative-stress-parameters-and-inflammatory-and-immune-mediators-as-markers-of-the-severity-of-sepsis
#12
Isil Bavunoglu, Habibe Genc, Dildar Konukoglu, Hayriye Cicekci, Volkan Sozer, Remise Gelisgen, Hafize Uzun
INTRODUCTION: Sepsis is a complex inflammatory syndrome with diverse etiology and wide spectrum of severity. The aim of this study was to investigate whether inflammatory mediators, in comparison with oxidative parameters, are associated with severity of sepsis. METHODOLOGY: Plasma neopterin, adenosine deaminase (ADA), vascular cell adhesion molecule (VCAM), intracellular adhesion molecule (ICAM), interleukin (IL)-1, IL-6, and tumor necrosis factor alpha (TNF-α), as inflammatory mediators, and serum nitric oxide (NOx), nitrotyrosine (NT), oxidized LDL (oxLDL) levels, serum paraoxonase 1 (PON1) activity, and erythrocyte glutathione (GSH) levels as oxidative stress parameters of 12 patients with mild sepsis, 25 patients with severe sepsis, and 20 healthy control subjects were evaluated...
October 31, 2016: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/27693409/serum-paraoxonase-activity-in-relation-to-lipid-profile-in-age-related-macular-degeneration-patients
#13
Kannadasan AnandBabu, S R Bharathidevi, Sarangapani Sripriya, Parveen Sen, Vadivelu Jaya Prakash, Appukuttan Bindu, Natarajan Viswanathan, Narayanasamy Angayarkanni
Age-related Macular Degeneration (AMD) is a multifactorial disease causing visual impairment in old age. Oxidative stress is one of the main contributors for the disease progression. Paraoxonase (PON), a HDL-resident antioxidant enzyme which removes oxidized low density lipoprotein (oxLDL), which is not studied much in AMD. This study assesses the PON activities in relation to the lipid status and genetic variants in AMD patients. In this prospective case-control study, a total of 48 AMD patients and 30 unrelated healthy controls were recruited...
September 28, 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/27682002/oxidized-ldl-insulin-resistance-and-central-blood-pressure-after-gestational-diabetes-mellitus
#14
Tiina Vilmi-Kerälä, Outi Palomäki, Päivi Kankkunen, Leena Juurinen, Jukka Uotila, Ari Palomäki
INTRODUCTION: Gestational diabetes mellitus (GDM) is an indicator of future cardiovascular disease. We investigated whether sensitive biomarkers of increased cardiovascular risk differ between women with and without a history of GDM few years after pregnancy, and whether obesity affects the results. MATERIAL AND METHODS: We studied two cohorts - 120 women with a history of GDM and 120 controls, on average 3.7 years after delivery. Circulating concentrations of oxidized low-density lipoprotein (oxLDL) were determined by ELISA...
December 2016: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/27667446/oxidized-ldl-statin-use-morbidity-and-mortality-in-patients-receiving-maintenance-hemodialysis
#15
Sandra Wagner, Mugurel Apetrii, Ziad A Massy, Marcus E Kleber, Graciela E Delgado, Hubert Scharnagel, Winfried März, Marie Metzger, Patrick Rossignol, Alan Jardine, Hallvard Holdaas, Bengt Fellström, Roland Schmieder, Bénédicte Stengel, Faiez Zannad
Statin treatment reduces the risk of cardiovascular mortality in the general population, but it has little or no benefit in hemodialyzed (HD) patients. This may reflect different underlying pathophysiology of cardiovascular disease (CVD) in patients treated with HD, maybe involving the oxidative stress. Our aim was to assess the association of oxidized low-density lipoprotein (oxLDL), determined by Mercodia oxLDL enzyme-linked immunosorbent assay (ELISA) kit, with major adverse cardiac events (MACE) and all-cause mortality in HD patients based on the AURORA trial (rosuvastatin vs placebo), and patients not on HD from the Ludwigshafen Risk and Cardiovascular Health (LURIC) study...
January 2017: Free Radical Research
https://www.readbyqxmd.com/read/27578220/association-between-adjunctive-metformin-therapy-in-young-type-1-diabetes-patients-with-excess-body-fat-and-reduction-of-carotid-intima-media-thickness
#16
Paweł Burchardt, Agnieszka Zawada, Jolanta Kaczmarek, Justyna Marcinkaniec, Henryk Wysocki, Bogna Wierusz-Wysocka, Marian Grzymisławski, Janusz Rzeźniczak, Dorota Zozulińska-Ziółkiewicz, Dariusz Naskręt
INTRODUCTION    Lipoprotein-associated phospholipase A2 (Lp-PLA2) and cholesteryl ester lipase (CEL) may oxidize low-density lipoproteins (oxLDL). OBJECTIVES    The aim of the study was to determine the influence of metformin on the metabolism of atherogenic lipid fractions in relation to Lp-PLA2 and CEL levels, as well as assess consequent improvement in the intima-media thickness (IMT) of the common carotid artery in young type 1 diabetes patients with excess body fat. PATIENTS AND METHODS    It was an open-label randomized clinical trial that lasted 6 months...
August 25, 2016: Polskie Archiwum Medycyny Wewnętrznej
https://www.readbyqxmd.com/read/27569284/knockdown-of-fstl1-inhibits-oxldl-induced-inflammation-responses-through-the-tlr4-myd88-nf-%C3%AE%C2%BAb-and-mapk-pathway
#17
Junxia Guo, Wanqian Liang, Jianhua Li, Jingning Long
Activation of inflammation by oxidized low-density lipoprotein (oxLDL) has been implicated in the development of atherosclerosis. Follistatin-like protein 1 (FSTL1) play a central role in the inflammation process and modulate cardiovascular disorders. However, little is known about the effects of FSTL1 on the inflammation induced by oxLDL. The aim of this study was to evaluate the anti-inflammatory effect of FSTL1 and investigate potential mechanisms in cultured endothelial cells. A model of oxLDL-induced injury in human coronary artery endothelial cells (HCAECs) was established to evaluate the protective role of FSTL1...
September 30, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27470353/xiaoxianggou-attenuates-atherosclerotic-plaque-formation-in-endogenous-high-ang-ii-apoe-mice-via-the-inhibition-of-mir-203-on-the-expression-of-ets-2-in-endothelial-cells
#18
Wencheng Nie, Xiaoqun Zhang, Hui Yan, Shan Li, Weiguo Zhu, Fangyan Fan, Jianhua Zhu
BACKGROUND: Atherosclerosis is a chronic immune-inflammatory disorder and one of the leading causes responsible for cardiovascular morbidity and mortality. Traditional Chinese medicine treatment with multi-targets has shown prospects for the therapeutic effect on atherosclerosis. Thus, this study aims to investigate whether xiaoxianggou has benefit for reducing the atherosclerotic plaque area in endogenous high Ang II ApoE(-/-) mice and investigated the underlying mechanisms. METHODS: Endogenous high Ang II ApoE(-/-) mice model was generated by using two kidney one clip (2K1C)...
August 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27421852/increased-risk-of-cardiovascular-disease-in-premenopausal-female-ragpickers-of-eastern-india-involvement-of-inflammation-oxidative-stress-and-platelet-hyperactivity
#19
Nandan Kumar Mondal, Sanghita Roychoudhury, Sayali Mukherjee, Shabana Siddique, Madhuchanda Banerjee, Mark S Slaughter, Twisha Lahiri, Manas Ranjan Ray
Millions of poor people in the developing world still thrive on ragpicking. In the present study, we have examined whether ragpicking is associated with increased risk of cardiovascular disease. For this, we have enrolled 112 premenopausal female ragpickers (median age 30 years) and 98 age-matched housemaids as control from Kolkata, Eastern India. Venous blood was drawn for routine hematology; flow cytometry was used to measure generation of reactive oxygen species (ROS) by leukocytes, surface expression of CD62P (P-selectin) in platelets and CD11b in leukocytes...
August 2016: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/27416002/novel-immune-assay-for-quantification-of-plasma-protective-capacity-against-oxidized-phospholipids
#20
Valery Bochkov, Andreas W Schoenenberger, Olga Oskolkova, Ursula Toth, Johannes Stöckl, Otto Majdic, Armond Daci, Thérèse J Resink, Paul Erne, Maria Philippova
AIM: Oxidized phospholipids (OxPL) are the major pathogenic component of oxidized low-density lipoproteins (OxLDL). Endogenous anti-OxPL activity, defined as the ability to neutralize adverse effects of oxidized lipids, may have biomarker potential. METHODS & RESULTS: Using two anti-OxPL monoclonal antibodies (commercial mAB-E06 and custom mAB-509) we developed a novel ELISA that measures the global capacity of plasma to inactivate OxPL. Preincubation of OxLDL with plasma inhibits its binding of anti-OxPL mABs...
August 2016: Biomarkers in Medicine
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