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oxldl elisa

Kannadasan AnandBabu, S R Bharathidevi, Sarangapani Sripriya, Parveen Sen, Vadivelu Jaya Prakash, Appukuttan Bindu, Natarajan Viswanathan, Narayanasamy Angayarkanni
Age-related Macular Degeneration (AMD) is a multifactorial disease causing visual impairment in old age. Oxidative stress is one of the main contributors for the disease progression. Paraoxonase (PON), a HDL-resident antioxidant enzyme which removes oxidized low density lipoprotein (oxLDL), which is not studied much in AMD. This study assesses the PON activities in relation to the lipid status and genetic variants in AMD patients. In this prospective case-control study, a total of 48 AMD patients and 30 unrelated healthy controls were recruited...
September 28, 2016: Experimental Eye Research
Tiina Vilmi-Kerälä, Outi Palomäki, Päivi Kankkunen, Leena Juurinen, Jukka Uotila, Ari Palomäki
INTRODUCTION: Gestational diabetes mellitus (GDM) is an indicator of future cardiovascular disease. We investigated if sensitive biomarkers of increased cardiovascular risk differ between women with and without a history of GDM few years after pregnancy, and whether obesity affects the results. MATERIAL AND METHODS: We studied two cohorts - 120 women with a history of GDM and 120 controls, on average 3.7 years after delivery. Circulating concentrations of oxidized low-density lipoprotein (oxLDL) were determined by ELISA...
September 28, 2016: Acta Obstetricia et Gynecologica Scandinavica
Sandra Wagner, Mugurel Apetrii, Ziad A Massy, Kleber E Marcus, Graciela E Delgado, Scharnagel Hubert, März Winfried, Marie Metzger, Patrick Rossignol, Alan Jardine, Hallvard Holdaas, Bengt Fellström, Roland Schmieder, Bénédicte Stengel, Faiez Zannad
Statin treatment reduces the risk of cardiovascular mortality in the general population, but it has little or no benefit in hemodialyzed patients (HD). This may reflect different underlying pathophysiology of CVD in patients treated with HD, maybe involving the oxidative stress. Our aim was to assess the association of oxidized LDL (oxLDL), determinated by Mercodia oxLDL ELISA Kit, with major adverse cardiac events (MACE) and all-cause mortality in HD patients based on the AURORA trial (rosuvastatin vs placebo), and patients not on HD from the LURIC study...
September 25, 2016: Free Radical Research
Paweł Burchardt, Agnieszka Zawada, Jolanta Kaczmarek, Justyna Marcinkaniec, Henryk Wysocki, Bogna Wierusz-Wysocka, Marian Grzymisławski, Janusz Rzeźniczak, Dorota Zozulińska-Ziółkiewicz, Dariusz Naskręt
INTRODUCTION    Lipoprotein-associated phospholipase A2 (Lp-PLA2) and cholesteryl ester lipase (CEL) may oxidize low-density lipoproteins (oxLDL). OBJECTIVES    The aim of the study was to determine the influence of metformin on the metabolism of atherogenic lipid fractions in relation to Lp-PLA2 and CEL levels, as well as assess consequent improvement in the intima-media thickness (IMT) of the common carotid artery in young type 1 diabetes patients with excess body fat. PATIENTS AND METHODS    It was an open-label randomized clinical trial that lasted 6 months...
August 25, 2016: Polskie Archiwum Medycyny Wewnętrznej
Junxia Guo, Wanqian Liang, Jianhua Li, Jingning Long
Activation of inflammation by oxidized low-density lipoprotein (oxLDL) has been implicated in the development of atherosclerosis. Follistatin-like protein 1 (FSTL1) play a central role in the inflammation process and modulate cardiovascular disorders. However, little is known about the effects of FSTL1 on the inflammation induced by oxLDL. The aim of this study was to evaluate the anti-inflammatory effect of FSTL1 and investigate potential mechanisms in cultured endothelial cells. A model of oxLDL-induced injury in human coronary artery endothelial cells (HCAECs) was established to evaluate the protective role of FSTL1...
September 30, 2016: Biochemical and Biophysical Research Communications
Wencheng Nie, Xiaoqun Zhang, Hui Yan, Shan Li, Weiguo Zhu, Fangyan Fan, Jianhua Zhu
BACKGROUND: Atherosclerosis is a chronic immune-inflammatory disorder and one of the leading causes responsible for cardiovascular morbidity and mortality. Traditional Chinese medicine treatment with multi-targets has shown prospects for the therapeutic effect on atherosclerosis. Thus, this study aims to investigate whether xiaoxianggou has benefit for reducing the atherosclerotic plaque area in endogenous high Ang II ApoE(-/-) mice and investigated the underlying mechanisms. METHODS: Endogenous high Ang II ApoE(-/-) mice model was generated by using two kidney one clip (2K1C)...
August 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Nandan Kumar Mondal, Sanghita Roychoudhury, Sayali Mukherjee, Shabana Siddique, Madhuchanda Banerjee, Mark S Slaughter, Twisha Lahiri, Manas Ranjan Ray
Millions of poor people in the developing world still thrive on ragpicking. In the present study, we have examined whether ragpicking is associated with increased risk of cardiovascular disease. For this, we have enrolled 112 premenopausal female ragpickers (median age 30 years) and 98 age-matched housemaids as control from Kolkata, Eastern India. Venous blood was drawn for routine hematology; flow cytometry was used to measure generation of reactive oxygen species (ROS) by leukocytes, surface expression of CD62P (P-selectin) in platelets and CD11b in leukocytes...
August 2016: Molecular and Cellular Biochemistry
Valery Bochkov, Andreas W Schoenenberger, Olga Oskolkova, Ursula Toth, Johannes Stöckl, Otto Majdic, Armond Daci, Thérèse J Resink, Paul Erne, Maria Philippova
AIM: Oxidized phospholipids (OxPL) are the major pathogenic component of oxidized low-density lipoproteins (OxLDL). Endogenous anti-OxPL activity, defined as the ability to neutralize adverse effects of oxidized lipids, may have biomarker potential. METHODS & RESULTS: Using two anti-OxPL monoclonal antibodies (commercial mAB-E06 and custom mAB-509) we developed a novel ELISA that measures the global capacity of plasma to inactivate OxPL. Preincubation of OxLDL with plasma inhibits its binding of anti-OxPL mABs...
August 2016: Biomarkers in Medicine
Kevin P Bliden, Rahul Chaudhary, Luis R Lopez, Rongras Damrongwatanasuk, Kirk Guyer, Martin G Gesheff, Christopher J Franzese, Himabindu Kaza, Udaya S Tantry, Paul A Gurbel
Oxidized low-density lipoprotein (oxLDL) and β2-glycoprotein I (β2GPI) have been identified in human atherosclerotic lesions and when complexed have been implicated as a pro-atherothrombotic antigen. We examined the association of free oxLDL and oxLDL-β2GPI complex in patients with coronary artery disease who underwent elective cardiac catheterization. Serum was collected from patients with suspected coronary artery disease immediately before elective cardiac catheterization who were either treated (n = 385) or not treated (n = 150) with statins and from healthy volunteers (n = 134)...
September 1, 2016: American Journal of Cardiology
Rahul Chaudhary, Kevin P Bliden, Jalaj Garg, Nafees Mohammed, Udaya Tantry, Denny Mathew, Peter P Toth, Christopher Franzese, Martin Gesheff, Shachi Pandya, Paul Gurbel
BACKGROUND: Statin and aspirin form the therapeutic cornerstone in patients with coronary artery disease (CAD) and diabetes. Little is known about relationship of statins with blood thrombogenicity and inflammation in these patients. METHODS: Two hundred nine consecutive patients with diabetes and suspected CAD undergoing elective cardiac catheterization were divided in groups based on statin treatment in the Multi-Analyte, Thrombogenic, and Genetic Markers Atherosclerosis study...
September 2016: Journal of Diabetes and its Complications
Xiao Qun Wang, Zhu Hui Liu, Lu Xue, Lin Lu, Jie Gao, Ying Shen, Ke Yang, Qiu Jing Chen, Rui Yan Zhang, Wei Feng Shen
BACKGROUND AND AIMS: Macrophage is a major contributor to the development of atherosclerosis by taking up deposited lipoprotein and eliciting local inflammation. Previously, we and others have shown C1q/TNF-related proteins (CTRPs) play diverse roles in vascular functions. In this study, we sought to investigate the changes in CTRP expression levels during vital biological processes in macrophages and their relation to inflammatory responses. METHODS: Western blot and real-time PCR were performed to analyze CTRPs expression levels in human peripheral blood mononuclear cells, primary macrophages and lipid-laden foam cells...
July 2016: Atherosclerosis
Miyuki Kumano-Kuramochi, Takashi Fujimura, Shiro Komba, Mari Maeda-Yamamoto, Sachiko Machida
Increased levels of oxidized low-density lipoprotein (OxLDL) in the blood circulation are correlated with atherosclerosis. Monoclonal antibody-based detection systems have been reported for OxLDL. We identified novel single-chain variable fragments (scFvs) having affinity for human OxLDL and related ligands. We constructed an scFv library from nonimmunized human spleen mRNA. Two types (γ+κ and μ+λ) of scFv phage libraries were enriched by biopanning, and five scFv clones with affinity for OxLDL were identified...
September 2016: Journal of Bioscience and Bioengineering
Henry Nording, Annika Giesser, Johannes Patzelt, Reinhard Sauter, Frederic Emschermann, Konstantinos Stellos, Meinrad Gawaz, Harald F Langer
Both oxidized lipids as well as the complement system contribute to atherothrombosis. The expression of complement receptors correlates with the expression of platelet activation markers, and platelet bound oxidized low-density lipoprotein (oxLDL) modulates platelet function. In the present study, we investigated the relationship of markers of complement activation, the anaphylatoxins C5a and C3a, and oxidized low-density lipoprotein. Two hundred and seven patients with coronary artery disease (CAD) were analyzed in this study...
March 30, 2016: Platelets
Wen Qin, Wei Xie, Ning Xia, Qinglin He, Tianwei Sun
BACKGROUND: Transient receptor potential channel 4 (TRPC4) plays central roles in endothelial cell function. The aim of this study was to investigate the silencing effects of TRPC4 on oxidized low-density lipoprotein (oxLDL)-induced angiogenesis in human coronary artery endothelial cells (HCAECs), as well as the underlying molecular mechanism involved in this process. MATERIAL/METHODS: HCAECs were transfected with small interfering RNA (siRNA) targeting TRPC4 (TRPC4-siRNA) or with a negative control (NC)-siRNA...
2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Javier Ampuero, Isidora Ranchal, Rocío Gallego-Durán, María Jesús Pareja, Jose Antonio Del Campo, Helena Pastor-Ramírez, María Carmen Rico, Rocío Picón, Luis Pastor, Carmelo García-Monzón, Raúl Andrade, Manuel Romero-Gómez
BACKGROUND AND AIM: A small but significant proportion of patients with normal body mass index show non-alcoholic fatty liver disease (NAFLD). Oxidized low-density lipoprotein (LDL) is a powerful immunogenic molecule, which causes oxidative stress and produces antibodies (oxLDL-ab). We aimed to analyze the role of oxLDL-ab on histological features in lean-NAFLD patients. METHODS: Seventy-two biopsy-proven NAFLD patients were included. Lean patients showed body index mass of <30 kg/m(2) ...
September 2016: Journal of Gastroenterology and Hepatology
Molly E Harmon, Matthew J Campen, Curtis Miller, Chris Shuey, Miranda Cajero, Selita Lucas, Bernadette Pacheco, Esther Erdei, Sandy Ramone, Teddy Nez, Johnnye Lewis
The prevalences of cardiovascular disease (CVD) and type 2 diabetes (T2D) have increased among the Navajo Native American community in recent decades. Oxidized low-density lipoprotein (oxLDL) is a novel CVD biomarker that has never been assessed in the Navajo population. We examined the relationship of oxLDL to conventional CVD and T2D risk factors and biomarkers in a cross-sectional population of Navajo participants. This cross-sectional study included 252 participants from 20 Navajo communities from the Diné Network for Environmental Health Project...
2016: PloS One
Jonathan Watt, Simon Kennedy, Nadeem Ahmed, James Hayhurst, John D McClure, Colin Berry, Roger M Wadsworth, Keith G Oldroyd
OBJECTIVE: The balance between coronary endothelial dysfunction and repair is influenced by many protective and deleterious factors circulating in the blood. We studied the relationship between oxidised low-density lipoprotein (oxLDL), circulating endothelial progenitor cells (EPCs) and coronary endothelial function in patients with stable coronary heart disease (CHD). METHODS: 33 patients with stable CHD were studied. Plasma oxLDL was measured using ELISA, coronary endothelial function was assessed using intracoronary acetylcholine infusion and EPCs were quantified using flow cytometry for CD34(+)/KDR(+) cells...
2016: Open Heart
Jonatas Zeni Klafke, Roberta Lelis Dias Pereira, Gabriela Elisa Hirsch, Mariana Migliorini Parisi, Fernando Garcez Porto, Amanda Spring de Almeida, Fabiane Horbach Rubin, Aline Schmidt, Henrique Beutler, Sabrina Nascimento, Gabriela Trevisan, Indiara Brusco, Sara Marchesan de Oliveira, Marta Maria Medeiros Frescura Duarte, Thiago Duarte, Paulo Ricardo Nazário Viecili
BACKGROUND: Atherosclerosis is an inflammatory disease that affects the arterial wall leading to myocardial, cerebral, and peripheral ischemic syndromes. The use of low doses of aspirin inhibits platelet aggregation and inflammation and prevents cardiovascular mortality. However, ASA may produce hemorrhagic events. Thus, several studies have sought new natural compounds to suppress platelet aggregation without causing serious adverse effects. PURPOSE: In this sense, this study aims to compare the effects of Campomanesia xanthocarpa plant extract with those of acetylsalicylic acid (ASA) on inflammatory parameters observed in homozygous mice knockout for the low-density lipoprotein receptor (LDLr-KO) treated with a hypercholesterolemic diet...
October 15, 2016: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Jun-Jie Wang, Xiao-Lan Chen, Cang-Bao Xu, Gao-Feng Jiang, Jie Lin, En-Qi Liu, Xu-Ping Qin, Jie Li
Minimally modified low density lipoprotein (mmLDL) is a risk factor for cardiovascular diseases. However, no studies examining the effect of mmLDL on vascular smooth muscle receptors have been released. The current study investigated the effect of mmLDL on the mesenteric artery α1 adrenoceptor and the molecular mechanisms. Mice were divided into the normal saline (NS), mmLDL, and mmLDL+U0126 groups. In the mmLDL+U0126 group, the animals were subjected to an intravenous tail injection of mmLDL and an intraperitoneal injection of U0126...
February 2016: Vascular Pharmacology
K Paapstel, M Zilmer, J Eha, K Tootsi, A Piir, J Kals
BACKGROUND: Fibulin-1 (FBLN-1), a newly identified biomarker for vascular stiffness in type 2 diabetes, may participate in the pathophysiological processes leading to progression of arterial stiffness in atherosclerosis. In the present study, the relationship between FBLN-1 and arterial stiffness was examined in patients with atherosclerosis and in healthy subjects. METHODS: Thirty-eight patients with peripheral arterial disease (PAD) (age 62.4 ± 9.0 years), 38 patients with coronary artery disease (CAD) (age 64...
January 2016: European Journal of Vascular and Endovascular Surgery
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