keyword
MENU ▼
Read by QxMD icon Read
search

Proteinase activated receptor

keyword
https://www.readbyqxmd.com/read/27931798/differential-expression-of-low-density-lipoprotein-receptor-related-protein-1-lrp-1-and-matrix-metalloproteinase-9-mmp-9-in-prostate-gland-from-normal-to-malignant-lesions
#1
Mónica B Gilardoni, María M Remedi, Mabel Oviedo, Tristán Dellavedova, Juan P Sarría, Laura Racca, Mariana Dominguez, Claudia G Pellizas, Ana C Donadio
BACKGROUND: Metalloproteinases (MMPs) are relevant modulators of inflammation, tumor microenvironment, cancer invasion and metastasis. They can be regulated by the Low density lipoprotein Receptor-related Protein 1 (LRP-1), a receptor reported to mediate the clearance of lipoproteins, extracellular matrix (ECM) macromolecules and proteinases. The aim of this study was to evaluate the expression of LRP-1, MMP-2 and MMP-9 across various grades of prostatic diseases as benign prostatic hyperplasia (BPH), BPH plus prostatitis (BPH+P), high grade prostatic intraepithelial neoplasia (HGPIN) and prostate cancer (PCa)...
November 24, 2016: Pathology, Research and Practice
https://www.readbyqxmd.com/read/27917624/relationships-between-high-mobility-group-protein-b1-and-triggering-receptor-expressed-on-myeloid-cells-concentrations-in-gingival-crevicular-fluid-and-chronic-periodontitis
#2
Mojgan Paknejad, Mandana Sattari, Zohreh Roozbahani, Morteza Ershadi, Ali Mehrfard
One of the inflammatory mediators which is secreted by inflammatory cells is high-mobility group protein B1 (HMGB1). Interaction of HMGB1 and toll-like receptors (TLRs) leads to increased production of inflammatory cytokines. On the other hand, it was shown that triggering receptor expressed on myeloid cells (TREM-1) also can be activated by TLRs, and its soluble form (sTREM-1) can be formed by cleaving of membrane-bound form of TREM-1 proteinases. Since there is not enough knowledge about the precise role of HMGB1 and sTREM-1 in periodontal diseases, the aim of this study was to evaluate the concentration of HMGB1 and sTREM-1 in gingival crevicular fluid (GCF) samples of patients with chronic periodontitis...
October 2016: Iranian Journal of Allergy, Asthma, and Immunology
https://www.readbyqxmd.com/read/27916875/proteinase-activated-receptor-2-is-a-novel-regulator-of-tgf-%C3%AE-signaling-in-pancreatic-cancer
#3
REVIEW
David Witte, Franziska Zeeh, Thomas Gädeken, Frank Gieseler, Bernhard H Rauch, Utz Settmacher, Roland Kaufmann, Hendrik Lehnert, Hendrik Ungefroren
TGF-β has a dual role in tumorigenesis, acting as a tumor suppressor in normal cells and in the early stages of tumor development while promoting carcinogenesis and metastasis in advanced tumor stages. The final outcome of the TGF-β response is determined by cell-autonomous mechanisms and genetic alterations such as genomic instability and somatic mutations, but also by a plethora of external signals derived from the tumor microenvironment, such as cell-to-cell interactions, growth factors and extracellular matrix proteins and proteolytic enzymes...
November 30, 2016: Journal of Clinical Medicine
https://www.readbyqxmd.com/read/27906053/attenuated-ca-2-release-in-a-mouse-model-of-limb-girdle-muscular-dystrophy-2a
#4
Marino DiFranco, Irina Kramerova, Julio L Vergara, Melissa Jan Spencer
BACKGROUND: Mutations in CAPN3 cause limb girdle muscular dystrophy type 2A (LGMD2A), a progressive muscle wasting disease. CAPN3 is a non-lysosomal, Ca-dependent, muscle-specific proteinase. Ablation of CAPN3 (calpain-3 knockout (C3KO) mice) leads to reduced ryanodine receptor (RyR1) expression and abnormal Ca2+/calmodulin-dependent protein kinase II (Ca-CaMKII)-mediated signaling. We previously reported that Ca(2+) release measured by fura2-FF imaging in response to single action potential stimulation was reduced in old C3KO mice; however, the use of field stimulation prevented investigation of the mechanisms underlying this impairment...
February 24, 2016: Skeletal Muscle
https://www.readbyqxmd.com/read/27881952/tissue-type-plasminogen-activator-tpa-modulates-the-postsynaptic-response-of-cerebral-cortical-neurons-to-the-presynaptic-release-of-glutamate
#5
Valerie Jeanneret, Fang Wu, Paola Merino, Enrique Torre, Ariel Diaz, Lihong Cheng, Manuel Yepes
Tissue-type plasminogen activator (tPA) is a serine proteinase released by the presynaptic terminal of cerebral cortical neurons following membrane depolarization (Echeverry et al., 2010). Recent studies indicate that the release of tPA triggers the synaptic vesicle cycle and promotes the exocytosis (Wu et al., 2015) and endocytic retrieval (Yepes et al., 2016) of glutamate-containing synaptic vesicles. Here we used electron microscopy, proteomics, quantitative phosphoproteomics, biochemical analyses with extracts of the postsynaptic density (PSD), and an animal model of cerebral ischemia with mice overexpressing neuronal tPA to study whether the presynaptic release of tPA also has an effect on the postsynaptic terminal...
2016: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/27866332/decreased-proteinase-a-excretion-by-strengthening-its-vacuolar-sorting-and-weakening-its-constitutive-secretion-in-saccharomyces-cerevisiae
#6
Yefu Chen, Lulu Song, Yueran Han, Mingming Liu, Rui Gong, Weiwei Luo, Xuewu Guo, Dongguang Xiao
Proteinase A (PrA), encoded by PEP4 gene, is detrimental to beer foam stability. There are two transport pathways for the new synthesized PrA in yeast, sorting to the vacuole normally, or excreting out of the cells under stress conditions. They were designated as the Golgi-to-vacuole pathway and the constitutive secretory pathway, respectively. To reduce PrA excretion in some new way instead of its coding gene deletion, which had a negative effect on cell metabolism and beer fermentation, we modified the PrA transport based on these above two pathways...
November 19, 2016: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27854307/mast-cells-density-positive-to-tryptase-correlate-with-microvascular-density-in-both-primary-gastric-cancer-tissue-and-loco-regional-lymph-node-metastases-from-patients-that-have-undergone-radical-surgery
#7
Michele Ammendola, Rosario Sacco, Valeria Zuccalà, Maria Luposella, Rosa Patruno, Pietro Gadaleta, Nicola Zizzo, Cosmo Damiano Gadaleta, Giovambattista De Sarro, Giuseppe Sammarco, Mihai Oltean, Girolamo Ranieri
Mast Cells (MCs) play a role in immune responses and more recently MCs have been involved in tumoral angiogenesis. In particular MCs can release tryptase, a potent in vivo and in vitro pro-angiogenic factor via proteinase-activated receptor-2 (PAR-2) activation and mitogen-activated protein kinase (MAPK) phosphorylation. MCs can release tryptase following c-Kit receptor activation. Nevertheless, no data are available concerning the relationship among MCs Density Positive to Tryptase (MCDPT) and Microvascular Density (MVD) in both primary gastric cancer tissue and loco-regional lymph node metastases...
November 15, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27832188/melatonin-receptor-agonists-as-the-perioceutics-agents-for-periodontal-disease-through-modulation-of-porphyromonas-gingivalis-virulence-and-inflammatory-response
#8
Wei Zhou, Xuan Zhang, Cai-Lian Zhu, Zhi-Yan He, Jing-Ping Liang, Zhong-Chen Song
AIM: "Perioceutics" including antimicrobial therapy and host modulatory therapy has emerged as a vital adjunctive treatment of periodontal disease. Melatonin level was significantly reduced in patients with periodontal diseases suggesting melatonin could be applied as a potential "perioceutics" treatment of periodontal diseases. This study aims to investigate the effects of melatonin receptor agonists (melatonin and ramelteon) on Porphyromonas gingivalis virulence and Porphyromonas gingivalis-derived lipopolysaccharide (Pg-LPS)-induced inflammation...
2016: PloS One
https://www.readbyqxmd.com/read/27778531/basic-mechanisms-of-platelet-receptor-shedding
#9
Robert K Andrews, Elizabeth E Gardiner
Proteolytic shedding of the extracellular ectodomain of platelet receptors provides a key mechanism for irreversible loss of ligand-binding capacity, and for regulating platelet function in health and disease. Platelets derived from megakaryocytes are small anucleate cells in peripheral blood, with the ability to rapidly adhere, become activated, and secrete an array of procoagulant and proinflammatory factors at sites of vascular injury or disease, and to form a platelet aggregate (thrombus) which is not only critical in normal hemostasis and wound healing, but in atherothrombotic diseases including myocardial infarction and ischemic stroke...
October 25, 2016: Platelets
https://www.readbyqxmd.com/read/27775020/%C3%AE-glutamyltranspeptidase-is-an-endogenous-activator-of-toll-like-receptor-4-mediated-osteoclastogenesis
#10
Sawako Moriwaki, Takeshi Into, Keiko Suzuki, Mutsumi Miyauchi, Takashi Takata, Keigo Shibayama, Shumpei Niida
Chronic inflammation-associated bone destruction, which is observed in rheumatoid arthritis (RA) and periodontitis, is mediated by excessive osteoclastogenesis. We showed previously that γ-glutamyltranspeptidase (GGT), an enzyme involved in glutathione metabolism, acts as an endogenous activator of such pathological osteoclastogenesis, independent of its enzymatic activity. GGT accumulation is clinically observed in the joints of RA patients, and, in animals, the administration of recombinant GGT to the gingival sulcus as an in vivo periodontitis model induces an increase in the number of osteoclasts...
October 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27720932/hydrogen-sulfide-donors-alleviate-itch-secondary-to-the-activation-of-type-2-protease-activated-receptors-par-2-in-mice
#11
S A Coavoy-Sánchez, L Rodrigues, S A Teixeira, A G Soares, R Torregrossa, M E Wood, M Whiteman, S K P Costa, M N Muscará
Hydrogen sulfide (H2S) has been highlighted as an endogenous signaling molecule and we have previously found that it can inhibit histamine-mediated itching. Pruritus is the most common symptom of cutaneous diseases and anti-histamines are the usual treatment; however, anti-histamine-resistant pruritus is common in some clinical settings. In this way, the involvement of mediators other than histamine in the context of pruritus requires new therapeutic targets. Considering that the activation of proteinase-activated receptor 2 (PAR-2) is involved in pruritus both in rodents and humans, in this study we investigated the effect of H2S donors on the acute scratching behavior mediated by PAR-2 activation in mice, as well as some of the possible pharmacological mechanisms involved...
October 6, 2016: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/27690729/endothelin-1-receptor-drives-invadopodia-exploiting-how-%C3%AE-arrestin-1-guides-the-way
#12
Anna Bagnato, Laura Rosanò
Metastatization is a complex multistep process requiring fine-tuned regulated cytoskeleton re-modeling, mediated by the cross-talk of actin with interacting partners, such as the Rho GTPases. Our expanding knowledge of invadopodia, small invasive membrane protrusions composed of a core of F-actin, actin regulators and actin-binding proteins, and hotspots for secretion of extracellular matrix (ECM) proteinases, contributes to clarify critical steps of the metastatic program. Growth factor receptors and their intermediate signaling molecules, along with matrix adhesion and rigidity, pH and hypoxia, act as drivers of cytoskeleton changes and invadopodia formation...
October 3, 2016: Small GTPases
https://www.readbyqxmd.com/read/27683007/peroxynitrite-formed-during-a-transient-episode-of-brain-ischaemia-increases-endothelium-derived-hyperpolarization-type-dilations-in-thromboxane-prostaglandin-receptor-stimulated-rat-cerebral-arteries
#13
Y Onetti, A P Dantas, B Pérez, A J McNeish, E Vila, F Jiménez-Altayó
AIM: Increased thromboxane A2 and peroxynitrite are hallmarks of cerebral ischaemia/reperfusion (I/R). Stimulation of thromboxane/prostaglandin receptors (TP) attenuates endothelium-derived hyperpolarization (EDH). We investigated whether EDH-type middle cerebral artery (MCA) relaxations following TP stimulation are altered after I/R and the influence of peroxynitrite. METHODS: Vascular function was determined by wire myography after TP stimulation with the thromboxane A2 mimetic 9,11-dideoxy-9α, 11α -methano-epoxy prostaglandin F2α (U46619) in MCA of Sprague Dawley rats subjected to MCA occlusion (90 min)/reperfusion (24 h) or sham operation, and in non-operated (control) rats...
September 28, 2016: Acta Physiologica
https://www.readbyqxmd.com/read/27677721/proteinases-their-extracellular-targets-and-inflammatory-signaling
#14
Rithwik Ramachandran, Christophe Altier, Katerina Oikonomopoulou, Morley D Hollenberg
Given that over 2% of the human genome codes for proteolytic enzymes and their inhibitors, it is not surprising that proteinases serve many physiologic-pathophysiological roles. In this context, we provide an overview of proteolytic mechanisms regulating inflammation, with a focus on cell signaling stimulated by the generation of inflammatory peptides; activation of the proteinase-activated receptor (PAR) family of G protein-coupled receptors (GPCR), with a mechanism in common with adhesion-triggered GPCRs (ADGRs); and by proteolytic ion channel regulation...
October 2016: Pharmacological Reviews
https://www.readbyqxmd.com/read/27677554/binding-of-synthetic-lkekk-peptide-to-human-t-lymphocytes
#15
E V Navolotskaya, D V Zinchenko, Y A Zolotarev, A A Kolobov, V M Lipkin
The synthetic peptide LKEKK corresponding to sequence 16-20 of human thymosin-α1 and 131-135 of human interferon-α2 was labeled with tritium to specific activity 28 Ci/mol. The [3H]LKEKK bound with high affinity (Kd = 3.7 ± 0.3 nM) to donor blood T-lymphocytes. Treatment of cells with trypsin or proteinase K did not abolish [3H]LKEKK binding, suggesting the non-protein nature of the peptide receptor. The binding was inhibited by thymosin-α1, interferon-α2, and cholera toxin B subunit (Ki = 2.0 ± 0.3, 2...
August 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27634880/evidence-that-vitronectin-is-a-potent-migration-enhancing-factor-for-cancer-cells-chaperoned-by-fibrinogen-a-novel-view-of-the-metastasis-of-cancer-cells-to-low-fibrinogen-lymphatics-and-body-cavities
#16
Gabriela Schneider, Ewa Bryndza, Agata Poniewierska-Baran, Karol Serwin, Malwina Suszynska, Zachariah P Sellers, Michael L Merchant, Alagammai Kaliappan, Janina Ratajczak, Magda Kucia, Nichola C Garbett, Mariusz Z Ratajczak
Diluted (1%) plasma induces migration of malignant cell lines much more strongly than potent pro-metastatic factors. To characterize the factor(s) present in diluted plasma responsible for this phenomenon we performed i) heat inactivation, ii) dialysis, iii) proteinase K treatment, and iv) molecular size filtration studies. We found that this remarkable pro-migratory activity of diluted normal plasma is associated with a ~50-100-kD protein that interacts with GαI protein-coupled receptors and activates p42/44 MAPK and AKT signaling in target cells...
September 13, 2016: Oncotarget
https://www.readbyqxmd.com/read/27633089/tgf-%C3%AE-1-autocrine-signalling-and-enamel-matrix-components
#17
Saeko Kobayashi-Kinoshita, Yasuo Yamakoshi, Kazuo Onuma, Ryuji Yamamoto, Yoshinobu Asada
Transforming growth factor-β1 (TGF-β1) is present in porcine enamel extracts and is critical for proper mineralization of tooth enamel. Here, we show that the mRNA of latent TGF-β1 is expressed throughout amelogenesis. Latent TGF-β1 is activated by matrix metalloproteinase 20 (MMP20), coinciding with amelogenin processing by the same proteinase. Activated TGF-β1 binds to the major amelogenin cleavage products, particularly the neutral-soluble P103 amelogenin, to maintain its activity. The P103 amelogenin-TGF-β1 complex binds to TGFBR1 to induce TGF-β1 signalling...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27625162/proteinase-activated-receptor-2-counterbalances-the-vascular-effects-of-endothelin-1-in-fibrotic-tight-skin-mice
#18
Fiorentina Roviezzo, Vincenzo Brancaleone, Valentina Mattera Iacono, Antonio Bertolino, Giovanna De Cunto, Valentina Vellecco, Giuseppe Lungarella, Monica Lucattelli, Giuseppe Cirino
BACKGROUND AND PURPOSE: The majority of the severe vascular complications in fibrosis are a consequence of a deregulated activity of mediators controlling vasomotor tone. One of the most important of these mediators is endothelin-1 (ET-1). Here, we have investigated the role of proteinase-activated receptor 2 (PAR2) in the vascular dysfunction in a model of fibrosis, using tight-skin (Tsk) mice. EXPERIMENTAL APPROACH: Aortas were collected from Tsk, transgenic over-expressing PAR2 (TgPAR2), PAR2 deficient (PAR2(-/-) ) or the corresponding WT mice...
September 14, 2016: British Journal of Pharmacology
https://www.readbyqxmd.com/read/27587403/neutrophils-can-disarm-nk-cell-response-through-cleavage-of-nkp46
#19
Alexandre Valayer, Deborah Brea, Laurie Lajoie, Leslie Avezard, Lucie Combes-Soia, Valerie Labas, Brice Korkmaz, Gilles Thibault, Thomas Baranek, Mustapha Si-Tahar
Polymorphonuclear neutrophils (PMNs) can contribute to the regulation of the host immune response by crosstalk with innate and adaptive leukocytes, including NK cells. Mechanisms by which this immunoregulation process occurs remain incompletely understood. Here, we focused on the effect of human neutrophil-derived serine proteases on NKp46, a crucial activating receptor expressed on NK cells. We used flow cytometry, Western blotting, and mass spectrometry (MS) analysis to reveal that cathepsin G [CG; and not elastase or proteinase 3 (PR3)] induces a time- and concentration-dependent, down-regulatory effect on NKp46 expression through a restricted proteolytic mechanism...
September 1, 2016: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/27566553/tgf%C3%AE-upregulates-par-1-expression-and-signalling-responses-in-a549-lung-adenocarcinoma-cells
#20
Natalia Smoktunowicz, Manuela Platé, Alejandro Ortiz Stern, Vanessa D'Antongiovanni, Eifion Robinson, Vijay Chudasama, Stephen Caddick, Chris J Scotton, Gabor Jarai, Rachel C Chambers
The major high-affinity thrombin receptor, proteinase activated receptor-1 (PAR-1) is expressed at low levels by the normal epithelium but is upregulated in many types of cancer, including lung cancer. The thrombin-PAR-1 signalling axis contributes to the activation of latent TGFβ in response to tissue injury via an αvβ6 integrin-mediated mechanism. TGFβ is a pleiotropic cytokine that acts as a tumour suppressor in normal and dysplastic cells but switches into a tumour promoter in advanced tumours. In this study we demonstrate that TGFβ is a positive regulator of PAR-1 expression in A549 lung adenocarcinoma cells, which in turn increases the sensitivity of these cells to thrombin signalling...
August 22, 2016: Oncotarget
keyword
keyword
84432
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"