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https://www.readbyqxmd.com/read/28822250/polyaspartate-extraction-of-cadmium-ions-from-contaminated-soil-evaluation-and-optimization-using-central-composite-design
#1
Nuhu Dalhat Mu'azu, Shamsudeen A Haladu, Nabeel Jarrah, Mukarram Zubair, Mohammad H Essa, Shaikh A Ali
The occurrences of heavy metal contaminated sites and soils and the need for devising environmentally friendly solutions have become global issues of serious concern. In this study, polyaspartate (a highly biodegradable agent) was synthesized using L-Aspartic acid via a new modified thermal procedure and employed for extraction of cadmium ions (Cd) from contaminated soil. Response surface methodology approach using 3(5) full faced centered central composite design was employed for modeling, evaluating and optimizing the influence of polyaspartate concentration (36-145mM), polyaspartate/soil ratio (5-25), initial heavy metal concentration (100-500mg/kg), initial pH (3-6) and extraction time (6-24h) on Cd ions extracted into the polyaspartate solution and its residual concentration in the treated soil...
August 8, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28822196/-metabolic-fingerprint-analysis-of-raw264-7-inflammatory-cell-model-by-using-uplc-q-tof-ms
#2
Shan-Shan Gao, Hui-Qing Guo, Ze-Kun Zhang, Guang-Can Bai, Xiao-Yan Gao, Chang-Hua Ma
In order to reveal the properties of polar metabolome in inflammatory cells, we selected LPS-induced RAW264.7 inflammatory cell models as the carrier for the research of metabolic fingerprint analysis. In this study, an ultra performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS)-based metabolomics protocol was optimized for the extraction of polar metabolites from RAW264.7 cell line. Then orthogonal partial least squares discriminant analysis (OPLS-DA) was used to process the metabolic data, and finally, a total of 17 metabolites were selected and identified...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822189/-determination-and-principal-component-analysis-of-mineral-elements-based-on-icp-oes-in-nitraria-roborowskii-fruits-from-different-regions
#3
Yuan-Yuan Yuan, Yu-Bi Zhou, Jing Sun, Juan Deng, Ying Bai, Jie Wang, Xue-Feng Lu
The content of elements in fifteen different regions of Nitraria roborowskii samples were determined by inductively coupled plasma-atomic emission spectrometry(ICP-OES), and its elemental characteristics were analyzed by principal component analysis. The results indicated that 18 mineral elements were detected in N. roborowskii of which V cannot be detected. In addition, contents of Na, K and Ca showed high concentration. Ti showed maximum content variance, while K is minimum. Four principal components were gained from the original data...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822134/genome-scale-modeling-of-photorespiratory-pathway-manipulation
#4
Anika Küken, Zoran Nikoloski
Quantifying the redistribution of metabolic reaction fluxes under experimental scenarios that affect the photorespiratory pathway can provide insights about the coupling of this pathway with other parts of metabolism. However, differential flux profiling on a genome-scale level remains the biggest challenge in modern systems biology. Here we present a protocol for applying a constraint-based approach, termed iReMet-Flux, that integrates data about relative metabolite levels in a stoichiometric metabolic model to predict differential fluxes at a genome-scale level under mild modeling assumptions...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28822122/estimation-of-photorespiratory-fluxes-by-gas-exchange
#5
Florian A Busch, Ross M Deans, Meisha-Marika Holloway-Phillips
Photorespiratory fluxes can be easily estimated by photosynthetic gas exchange using an infrared gas analyzer and applying the Farquhar, von Caemmerer, and Berry (Farquhar et al. Planta 149:78-90, 1980) photosynthesis model. For a more direct measurement of photorespiratory CO2 release from glycine decarboxylation, infrared gas analysis can be coupled to membrane-inlet mass spectrometry, capable of separating the total CO2 concentration into its (12)CO2 and (13)CO2 components in a continuous online fashion...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28822082/a-linear-ion-trap-with-an-expanded-inscribed-diameter-to-improve-optical-access-for-fluorescence-spectroscopy
#6
Vaishnavi Rajagopal, Chris Stokes, Alessandra Ferzoco
We report a custom-geometry linear ion trap designed for fluorescence spectroscopy of gas-phase ions at ambient to cryogenic temperatures. Laser-induced fluorescence from trapped ions is collected from between the trapping rods, orthogonal to the excitation laser that runs along the axis of the linear ion trap. To increase optical access to the ion cloud, the diameter of the round trapping rods is 80% of the inscribed diameter, rather than the roughly 110% used to approximate purely quadrupolar electric fields...
August 18, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28822068/polyethylene-damage-increases-with-varus-implant-alignment-in-posterior-stabilized-and-constrained-condylar-knee-arthroplasty
#7
Zhichang Li, Christina I Esposito, Chelsea N Koch, Yuo-Yu Lee, Douglas E Padgett, Timothy M Wright
BACKGROUND: Implant malalignment in primary TKA has been reported to increase stresses placed on the bearing surfaces of implant components. We used a longitudinally maintained registry coupled with an implant retrieval program to consider whether preoperative, postoperative, or prerevision malalignment was associated with increased risk of revision surgery after TKA. QUESTIONS/PURPOSES: (1) What is the relative polyethylene damage on medial and lateral compartments of the tibial plateaus from revised TKAs? (2) Does coronal TKA alignment affect implant performance, such that TKAs aligned in varus are predisposed to experience increased polyethylene damage? (3) Does TKA alignment differ between postoperative and prerevision radiographs, and if so, what does this difference suggest about the mechanical contact load placed on a knee with a TKA? METHODS: Between 2007 and 2012, we performed 18,065 primary TKAs at our institution...
August 18, 2017: Clinical Orthopaedics and related Research
https://www.readbyqxmd.com/read/28822038/translocator-protein-pet-imaging-in-a-preclinical-prostate-cancer-model
#8
Mohammed N Tantawy, H Charles Manning, Todd E Peterson, Daniel C Colvin, John C Gore, Wenfu Lu, Zhenbang Chen, C Chad Quarles
PURPOSE: The identification and targeting of biomarkers specific to prostate cancer (PCa) could improve its detection. Given the high expression of translocator protein (TSPO) in PCa, we investigated the use of [(18)F]VUIIS1008 (a novel TSPO-targeting radioligand) coupled with positron emission tomography (PET) to identify PCa in mice and to characterize their TSPO uptake. PROCEDURES: Pten(pc-/-), Trp53(pc-/-) prostate cancer-bearing mice (n = 9, 4-6 months old) were imaged in a 7T MRI scanner for lesion localization...
August 18, 2017: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/28821972/non-deformed-singular-and-non-singular-exponential-type-potentials
#9
J J Pe Na, G Ovando, J Morales, J García-Ravelo
The canonical transformation method applied to the Schrödinger equation to transform it into a second-order differential equation of hypergeometric-type is presented. Starting from there, those exactly solvable multiparameter exponential-type (ME-T) potentials with hypergeometric wavefunctions that belong to the families of radial (singular) and one-dimensional (non-singular) potentials, are obtained. Furthermore, we show how the choice of the involved parameters leads, as particular cases, to different deformed or non-deformed potential models already used in the study of electronic properties of diatomic molecules...
August 18, 2017: Journal of Molecular Modeling
https://www.readbyqxmd.com/read/28821871/fermi-surface-and-effective-masses-in-photoemission-response-of-the-ba1-x-k-x-fe2as2-superconductor
#10
Gerald Derondeau, Federico Bisti, Masaki Kobayashi, Jürgen Braun, Hubert Ebert, Victor A Rogalev, Ming Shi, Thorsten Schmitt, Junzhang Ma, Hong Ding, Vladimir N Strocov, Ján Minár
The angle-resolved photoemission spectra of the superconductor (Ba1-x K x )Fe2As2 have been investigated accounting coherently for spin-orbit coupling, disorder and electron correlation effects in the valence bands combined with final state, matrix element and surface effects. Our results explain the previously obscured origins of all salient features of the ARPES response of this paradigm pnictide compound and reveal the origin of the Lifshitz transition. Comparison of calculated ARPES spectra with the underlying DMFT band structure shows an important impact of final state effects, which result for three-dimensional states in a deviation of the ARPES spectra from the true spectral function...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821869/trpm6-and-trpm7-differentially-contribute-to-the-relief-of-heteromeric-trpm6-7-channels-from-inhibition-by-cytosolic-mg-2-and-mg%C3%A2-atp
#11
Silvia Ferioli, Susanna Zierler, Joanna Zaißerer, Johann Schredelseker, Thomas Gudermann, Vladimir Chubanov
TRPM6 and its homologue TRPM7 are α-kinase-coupled divalent cation-selective channels activated upon reduction of cytosolic levels of Mg(2+) and Mg·ATP. TRPM6 is vital for organismal Mg(2+) balance. However, mechanistically the cellular role and functional nonredundancy of TRPM6 remain incompletely understood. Comparative analysis of native currents in primary cells from TRPM6- versus TRPM7-deficient mice supported the concept that native TRPM6 primarily functions as a constituent of heteromeric TRPM6/7 channels...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821859/reduction-of-the-off-pathway-iron-sulphur-cluster-n1a-of-escherichia-coli-respiratory-complex-i-restrains-nad-dissociation
#12
Emmanuel Gnandt, Johannes Schimpf, Caroline Harter, Jo Hoeser, Thorsten Friedrich
Respiratory complex I couples the electron transfer from NADH to ubiquinone with the translocation of protons across the membrane. The reaction starts with NADH oxidation by a flavin cofactor followed by transferring the electrons through a chain of seven iron-sulphur clusters to quinone. An eighth cluster called N1a is located proximally to flavin, but on the opposite side of the chain of clusters. N1a is strictly conserved although not involved in the direct electron transfer to quinone. Here, we show that the NADH:ferricyanide oxidoreductase activity of E...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821847/overcoming-the-plasticity-of-plant-specialized-metabolism-for-selective-diterpene-production-in-yeast
#13
Codruta Ignea, Anastasia Athanasakoglou, Aggeliki Andreadelli, Maria Apostolaki, Minas Iakovides, Euripides G Stephanou, Antonios M Makris, Sotirios C Kampranis
Plants synthesize numerous specialized metabolites (also termed natural products) to mediate dynamic interactions with their surroundings. The complexity of plant specialized metabolism is the result of an inherent biosynthetic plasticity rooted in the substrate and product promiscuity of the enzymes involved. The pathway of carnosic acid-related diterpenes in rosemary and sage involves promiscuous cytochrome P450s whose combined activity results in a multitude of structurally related compounds. Some of these minor products, such as pisiferic acid and salviol, have established bioactivity, but their limited availability prevents further evaluation...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821830/disease-syndrome-combination-modeling-metabolomic-strategy-for-the-pathogenesis-of-chronic-kidney-disease
#14
Shasha Li, Peng Xu, Ling Han, Wei Mao, Yiming Wang, Guoan Luo, Nizhi Yang
Conventional disease animal models have limitations on the conformity to the actual clinical situation. Disease-syndrome combination (DS) modeling may provide a more efficient strategy for biomedicine research. Disease model and DS model of renal fibrosis in chronic kidney disease were established by ligating the left ureter and by ligating unilateral ureteral combined with exhaustive swimming, respectively. Serum metabolomics was conducted to evaluate disease model and DS model by using ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821802/dispersive-regimes-of-the-dicke-model
#15
Diego Barberena, Lucas Lamata, Enrique Solano
We study two dispersive regimes of the Dicke model in the dynamics of N two-level atoms interacting with a bosonic mode for long interaction times. Firstly, we analyze the model for the regime in which the qubit frequencies are equal and smaller than the mode frequency, and for values of the coupling strength similar or larger than the mode frequency, namely, the deep strong coupling regime. Secondly, we address an interaction that is dependent on the photon number, where the coupling strength is comparable to the geometric mean of the qubit and mode frequencies...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821764/helical-edge-states-and-edge-state-transport-in-strained-armchair-graphene-nanoribbons
#16
Zheng-Fang Liu, Qing-Ping Wu, Ai-Xi Chen, Xian-Bo Xiao, Nian-Hua Liu, Guo-Xing Miao
A helical type edge state, which is generally supported only on graphene with zigzag boundaries, is found to also appear in armchair graphene nanoribbons in the presence of intrinsic spin-orbit coupling and a suitable strain. At a critical strain, there appears a quantum phase transition from a quantum spin Hall state to a trivial insulator state. Further investigation shows that the armchair graphene nanoribbons with intrinsic spin-orbit coupling, Rashba spin-orbit coupling, effective exchange fields and strains also support helical-like edge states with a unique spin texture...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821763/activation-of-an-anti-bacterial-toxin-by-the-biosynthetic-enzyme-cysk-mechanism-of-binding-interaction-specificity-and-competition-with-cysteine-synthase
#17
Roberto Benoni, Christina M Beck, Fernando Garza-Sánchez, Stefano Bettati, Andrea Mozzarelli, Christopher S Hayes, Barbara Campanini
Contact-dependent growth inhibition (CDI) is a wide-spread mechanism of inter-bacterial competition. CDI(+) bacteria deliver CdiA-CT toxins into neighboring bacteria and produce specific immunity proteins that protect against self-intoxication. The CdiA-CT toxin from uropathogenic Escherichia coli 536 is a latent tRNase that is only active when bound to the cysteine biosynthetic enzyme CysK. Remarkably, the CysK:CdiA-CT binding interaction mimics the 'cysteine synthase' complex of CysK:CysE. The C-terminal tails of CysE and CdiA-CT each insert into the CysK active-site cleft to anchor the respective complexes...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821753/dynamic-reconfiguration-of-cortical-functional-connectivity-across-brain-states
#18
Iain Stitt, Karl J Hollensteiner, Edgar Galindo-Leon, Florian Pieper, Eva Fiedler, Thomas Stieglitz, Gerhard Engler, Guido Nolte, Andreas K Engel
Throughout each day, the brain displays transient changes in state, as evidenced by shifts in behavior and vigilance. While the electrophysiological correlates of brain states have been studied for some time, it remains unclear how large-scale cortico-cortical functional connectivity systematically reconfigures across states. Here, we investigate state-dependent shifts in cortical functional connectivity by recording local field potentials (LFPs) during spontaneous behavioral transitions in the ferret using chronically implanted micro-electrocorticographic (µECoG) arrays positioned over occipital, parietal, and temporal cortical regions...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821737/combining-%C3%AE%C2%B5-near-zero-behavior-and-stopped-light-energy-bands-for-ultra-low-reflection-and-reduced-dispersion-of-slow-light
#19
Frank Bello, A Freddie Page, Andreas Pusch, Joachim M Hamm, John F Donegan, Ortwin Hess
We investigate media which exhibits epsilon-near-zero (ENZ) behavior while simultaneously sustaining stopped light energy bands which contain multiple points of zero group velocity (ZGV). This allows the merging of state-of-the-art phenomena that was hitherto attainable in media that demonstrated these traits separately. Specifically, we demonstrate the existence of Ferrell-Berreman (FB) modes within frequency bands bounded by points of ZGV with the goal to improve the coupling efficiency and localization of light in the media...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821725/interplay-between-maternal-slc6a4-mutation-and-prenatal-stress-a-possible-mechanism-for-autistic-behavior-development
#20
Calvin P Sjaarda, Patrick Hecht, Amy J M McNaughton, Audrina Zhou, Melissa L Hudson, Matt J Will, Garth Smith, Muhammad Ayub, Ping Liang, Nansheng Chen, David Beversdorf, Xudong Liu
The low activity allele of the maternal polymorphism, 5HTTLPR, in the serotonin transporter, SLC6A4, coupled with prenatal stress is reported to increase the risk for children to develop autism spectrum disorder (ASD). Similarly, maternal Slc6a4 knock-out and prenatal stress in rodents results in offspring demonstrating ASD-like characteristics. The present study uses an integrative genomics approach to explore mechanistic changes in early brain development in mouse embryos exposed to this maternal gene-environment phenomenon...
August 18, 2017: Scientific Reports
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