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https://www.readbyqxmd.com/read/29740340/tissue-distribution-of-kir7-1-inwardly-rectifying-k-channel-probed-in-a-knock-in-mouse-expressing-a-haemagglutinin-tagged-protein
#1
Isabel Cornejo, Sandra Villanueva, Johanna Burgos, Karen I López-Cayuqueo, Régine Chambrey, Francisca Julio-Kalajzić, Neudo Buelvas, María I Niemeyer, Dulce Figueiras-Fierro, Peter D Brown, Francisco V Sepúlveda, L P Cid
Kir7.1 encoded by the Kcnj13 gene in the mouse is an inwardly rectifying K+ channel present in epithelia where it shares membrane localization with the Na+ /K+ -pump. Further investigations of the localisation and function of Kir7.1 would benefit from the availability of a knockout mouse, but perinatal mortality attributed to cleft palate in the neonate has thwarted this research. To facilitate localisation studies we now use CRISPR/Cas9 technology to generate a knock-in mouse, the Kir7.1-HA that expresses the channel tagged with a haemagglutinin (HA) epitope...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29649366/fgf2-enhances-odontoblast-differentiation-by-%C3%AE-sma-progenitors-in-vivo
#2
I Vidovic-Zdrilic, K H Vining, A Vijaykumar, I Kalajzic, D J Mooney, M Mina
The goal of this study was to examine the effects of early and limited exposure of perivascular cells expressing α (αSMA) to fibroblast growth factor 2 (FGF2) in vivo. We performed in vivo fate mapping by inducible Cre-loxP and experimental pulp injury in molars to induce reparative dentinogenesis. Our results demonstrate that early delivery of exogenous FGF2 to exposed pulp led to proliferative expansion of αSMA-tdTomato+ cells and their accelerated differentiation into odontoblasts. In vivo lineage-tracing experiments showed that the calcified bridge/reparative dentin in FGF2-treated pulps were lined with an increased number of Dspp+ odontoblasts and devoid of BSP+ osteoblasts...
April 1, 2018: Journal of Dental Research
https://www.readbyqxmd.com/read/29556234/the-long-pentraxin-3-plays-a-role-in-bone-turnover-and-repair
#3
Danka Grčević, Marina Sironi, Sonia Valentino, Livija Deban, Hrvoje Cvija, Antonio Inforzato, Nataša Kovačić, Vedran Katavić, Tomislav Kelava, Ivo Kalajzić, Alberto Mantovani, Barbara Bottazzi
Pentraxin 3 (PTX3) is an inflammatory mediator acting as a fluid-phase pattern recognition molecule and playing an essential role in innate immunity and matrix remodeling. Inflammatory mediators also contribute to skeletal homeostasis, operating at multiple levels in physiological and pathological conditions. This study was designed to investigate the role of PTX3 in physiological skeletal remodeling and bone healing. Micro-computed tomography (μCT) and bone histomorphometry of distal femur showed that PTX3 gene-targeted female and male mice ( ptx3-/- ) had lower trabecular bone volume than their wild-type ( ptx3+/+ ) littermates (BV/TV by μCT: 3...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29178402/micro-nanostructures-of-cellulose-collagen-for-critical-sized-bone-defect-healing
#4
Aja Aravamudhan, Daisy M Ramos, Jonathan Nip, Ivo Kalajzic, Sangamesh G Kumbar
Bone tissue engineering strategies utilize biodegradable polymeric matrices alone or in combination with cells and factors to provide mechanical support to bone, while promoting cell proliferation, differentiation, and tissue ingrowth. The performance of mechanically competent, micro-nanostructured polymeric matrices, in combination with bone marrow stromal cells (BMSCs), is evaluated in a critical sized bone defect. Cellulose acetate (CA) is used to fabricate a porous microstructured matrix. Type I collagen is then allowed to self-assemble on these microstructures to create a natural polymer-based, micro-nanostructured matrix (CAc)...
February 2018: Macromolecular Bioscience
https://www.readbyqxmd.com/read/29143340/k-2p-task-2-and-kcnq1-kcne3-k-channels-are-major-players-contributing-to-intestinal-anion-and-fluid-secretion
#5
Francisca Julio-Kalajzić, Sandra Villanueva, Johanna Burgos, Margarita Ojeda, L Pablo Cid, Thomas J Jentsch, Francisco V Sepúlveda
KEY POINTS: K+ channels are important in intestinal epithelium as they ensure the ionic homeostasis and electrical potential of epithelial cells during anion and fluid secretion. Intestinal epithelium cAMP-activated anion secretion depends on the activity of the (also cAMP dependent) KCNQ1-KCNE3 K+ channel, but the secretory process survives after genetic inactivation of the K+ channel in the mouse. Here we use double mutant mice to investigate which alternative K+ channels come into action to compensate for the absence of KCNQ1-KCNE3 K+ channels...
February 1, 2018: Journal of Physiology
https://www.readbyqxmd.com/read/28691752/mineral-trioxide-aggregate-improves-healing-response-of-periodontal-tissue-to-injury-in-mice
#6
I Vidovic Zdrilic, I O de Azevedo Queiroz, B G Matthews, J E Gomes-Filho, M Mina, I Kalajzic
BACKGROUND AND OBJECTIVE: Mineral trioxide aggregate (MTA) is a biomaterial used in endodontic procedures as it exerts beneficial effects on regenerative processes. In this study, we evaluate the effect of MTA on healing of periodontal ligament (PDL) and surrounding tissue, following injury, in a transgenic mouse model and on the differentiation of murine mesenchymal progenitor cells in vitro. MATERIAL AND METHODS: We used an inducible Cre-loxP in vivo fate mapping approach to examine the effects of MTA on the contributions of descendants of cells expressing the αSMA-CreERT2 transgene (SMA9+ ) to the PDL and alveolar bone after experimental injury to the root furcation on the maxillary first molars...
December 2017: Journal of Periodontal Research
https://www.readbyqxmd.com/read/28600151/splenomegaly-myeloid-lineage-expansion-and-increased-osteoclastogenesis-in-osteogenesis-imperfecta-murine
#7
Brya G Matthews, Emilie Roeder, Xi Wang, Hector Leonardo Aguila, Sun-Kyeong Lee, Danka Grcevic, Ivo Kalajzic
Osteogenesis imperfecta (OI) is a disease caused by defects in type I collagen production that results in brittle bones. While the pathology is mainly caused by defects in the osteoblast lineage, there is also elevated bone resorption by osteoclasts resulting in high bone turnover in severe forms of the disease. Osteoclasts originate from hematopoietic myeloid cells, however changes in hematopoiesis have not been previously documented in OI. In this study, we evaluated hematopoietic lineage distribution and osteoclast progenitor cell frequency in bone marrow, spleen and peripheral blood of osteogenesis imperfecta murine (OIM) mice, a model of severe OI...
October 2017: Bone
https://www.readbyqxmd.com/read/28594231/comparison-of-the-effects-of-three-surgical-techniques-on-the-rate-of-orthodontic-tooth-movement-in-a-rat-model
#8
Zachary Librizzi, Zana Kalajzic, Daniel Camacho, Sumit Yadav, Ravindra Nanda, Flavio Uribe
OBJECTIVE: To evaluate the effect of corticotomy and corticision, with and without a full mucoperiosteal flap, on the rate of tooth movement and alveolar response in a rat model. MATERIALS AND METHODS: Sixty male, 6-week-old Wistar rats were divided into five groups based on surgical procedure, as follows: control (no tooth movement), orthodontic tooth movement (OTM) only, corticotomy, corticision, and corticision with full mucoperiosteal flap (corticision + flap)...
September 2017: Angle Orthodontist
https://www.readbyqxmd.com/read/28297566/instructive-role-of-the-microenvironment-in-preventing-renal-fibrosis
#9
Kei Matsumoto, Sandhya Xavier, Jun Chen, Yujiro Kida, Mark Lipphardt, Reina Ikeda, Annie Gevertz, Mario Caviris, Antonis K Hatzopoulos, Ivo Kalajzic, James Dutton, Brian B Ratliff, Hong Zhao, Zbygniew Darzynkiewicz, Stefan Rose-John, Michael S Goligorsky
Accumulation of myofibroblasts is a hallmark of renal fibrosis. A significant proportion of myofibroblasts has been reported to originate via endothelial-mesenchymal transition. We initially hypothesized that exposing myofibroblasts to the extract of endothelial progenitor cells (EPCs) could reverse this transition. Indeed, in vitro treatment of transforming growth factor-β1 (TGF-β1)-activated fibroblasts with EPC extract prevented expression of α-smooth muscle actin (α-SMA); however, it did not enhance expression of endothelial markers...
March 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28170285/fgf-signaling-prevents-the-terminal-differentiation-of-odontoblasts
#10
K Sagomonyants, I Kalajzic, P Maye, M Mina
Members of the fibroblast growth factor (FGF) family play essential and important roles in primary and reparative dentinogenesis, with conflicting results regarding their effects on odontoblast differentiation. Our recent studies showed that the effects of FGF2 on cells in odontoblast lineage were stage-specific and depended on the stage of cell maturity. Continuous exposure of pulp cells to FGF2 inhibited odontoblast differentiation, whereas early and limited exposure of pulp cells to FGF2 resulted in marked increases in odontoblast differentiation...
June 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/27834664/%C3%AE-sma-expressing-perivascular-cells-represent-dental-pulp-progenitors-in-vivo
#11
I Vidovic, A Banerjee, R Fatahi, B G Matthews, N A Dyment, I Kalajzic, M Mina
The goal of this study was to examine the contribution of perivascular cells to odontoblasts during the development, growth, and repair of dentin using mouse molars as a model. We used an inducible, Cre-loxP in vivo fate-mapping approach to examine the contributions of the descendants of cells expressing the αSMA-CreERT2 transgene to the odontoblast lineage. In vivo lineage-tracing experiments in molars showed the contribution of αSMA-tdTomato+ cells to a small number of newly formed odontoblasts during primary dentinogenesis...
March 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/27723812/cellular-and-matrix-response-of-the-mandibular-condylar-cartilage-to-botulinum-toxin
#12
Eliane H Dutra, Mara H O' Brien, Alexandro Lima, Zana Kalajzic, Aditya Tadinada, Ravindra Nanda, Sumit Yadav
OBJECTIVES: To evaluate the cellular and matrix effects of botulinum toxin type A (Botox) on mandibular condylar cartilage (MCC) and subchondral bone. MATERIALS AND METHODS: Botox (0.3 unit) was injected into the right masseter of 5-week-old transgenic mice (Col10a1-RFPcherry) at day 1. Left side masseter was used as intra-animal control. The following bone labels were intraperitoneally injected: calcein at day 7, alizarin red at day 14 and calcein at day 21. In addition, EdU was injected 48 and 24 hours before sacrifice...
2016: PloS One
https://www.readbyqxmd.com/read/27721375/exploiting-endogenous-fibrocartilage-stem-cells-to-regenerate-cartilage-and-repair-joint-injury
#13
Mildred C Embree, Mo Chen, Serhiy Pylawka, Danielle Kong, George M Iwaoka, Ivo Kalajzic, Hai Yao, Chancheng Shi, Dongming Sun, Tzong-Jen Sheu, David A Koslovsky, Alia Koch, Jeremy J Mao
Tissue regeneration using stem cell-based transplantation faces many hurdles. Alternatively, therapeutically exploiting endogenous stem cells to regenerate injured or diseased tissue may circumvent these challenges. Here we show resident fibrocartilage stem cells (FCSCs) can be used to regenerate and repair cartilage. We identify FCSCs residing within the superficial zone niche in the temporomandibular joint (TMJ) condyle. A single FCSC spontaneously generates a cartilage anlage, remodels into bone and organizes a haematopoietic microenvironment...
October 10, 2016: Nature Communications
https://www.readbyqxmd.com/read/27708132/instructive-role-of-the-microenvironment-in-preventing-renal-fibrosis
#14
Kei Matsumoto, Sandhya Xavier, Jun Chen, Yujiro Kida, Mark Lipphardt, Reina Ikeda, Annie Gevertz, Mario Caviris, Antonis K Hatzopoulos, Ivo Kalajzic, James Dutton, Brian B Ratliff, Hong Zhao, Zbygniew Darzynkiewicz, Stefan Rose-John, Michael S Goligorsky
: Accumulation of myofibroblasts is a hallmark of renal fibrosis. A significant proportion of myofibroblasts has been reported to originate via endothelial-mesenchymal transition. We initially hypothesized that exposing myofibroblasts to the extract of endothelial progenitor cells (EPCs) could reverse this transition. Indeed, in vitro treatment of transforming growth factor-β1 (TGF-β1)-activated fibroblasts with EPC extract prevented expression of α-smooth muscle actin (α-SMA); however, it did not enhance expression of endothelial markers...
October 5, 2016: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/27616604/visual-reporters-for-study-of-the-osteoblast-lineage
#15
REVIEW
Emilie Roeder, Brya G Matthews, Ivo Kalajzic
Advancing our understanding of osteoblast biology and differentiation is critical to elucidate the pathological mechanisms responsible for skeletal diseases such as osteoporosis. Histology and histomorphometry, the classical methods to study osteoblast biology, identify osteoblasts based on their location and morphology and ability to mineralize matrix, but do not clearly define their stage of differentiation. Introduction of visual transgenes into the cells of osteoblast lineage has revolutionized the field and resulted in a paradigm shift that allowed for specific identification and isolation of subpopulations within the osteoblast lineage...
November 2016: Bone
https://www.readbyqxmd.com/read/27612450/increased-chemotaxis-and-activity-of-circulatory-myeloid-progenitor-cells-may-contribute-to-enhanced-osteoclastogenesis-and-bone-loss-in-the-c57bl-6-mouse-model-of-collagen-induced-arthritis
#16
M Ikić Matijašević, D Flegar, N Kovačić, V Katavić, T Kelava, A Šućur, S Ivčević, H Cvija, E Lazić Mosler, I Kalajzić, A Marušić, D Grčević
Our study aimed to determine the functional activity of different osteoclast progenitor (OCP) subpopulations and signals important for their migration to bone lesions, causing local and systemic bone resorption during the course of collagen-induced arthritis in C57BL/6 mice. Arthritis was induced with chicken type II collagen (CII), and assessed by clinical scoring and detection of anti-CII antibodies. We observed decreased trabecular bone volume of axial and appendicular skeleton by histomorphometry and micro-computed tomography as well as decreased bone formation and increased bone resorption rate in arthritic mice in vivo...
December 2016: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/27507737/quiescent-bone-lining-cells-are-a-major-source-of-osteoblasts-during-adulthood
#17
Igor Matic, Brya G Matthews, Xi Wang, Nathaniel A Dyment, Daniel L Worthley, David W Rowe, Danka Grcevic, Ivo Kalajzic
The in vivo origin of bone-producing osteoblasts is not fully defined. Skeletal stem cells, a population of mesenchymal stem cells resident in the bone marrow compartment, are thought to act as osteoprogenitors during growth and adulthood. Quiescent bone lining cells (BLCs) have been suggested as a population capable of activation into mature osteoblasts. These cells were defined by location and their morphology and studies addressing their significance have been hampered by their inaccessibility, and lack of markers that would allow for their identification and tracing...
December 2016: Stem Cells
https://www.readbyqxmd.com/read/27184388/murine-supraspinatus-tendon-injury-model-to-identify-the-cellular-origins-of-rotator-cuff-healing
#18
Ryu Yoshida, Farhang Alaee, Felix Dyrna, Mark S Kronenberg, Peter Maye, Ivo Kalajzic, David W Rowe, Augustus D Mazzocca, Nathaniel A Dyment
Purpose of this study: To elucidate the origin of cell populations that contribute to rotator cuff healing, we developed a mouse surgical model where a full-thickness, central detachment is created in the supraspinatus. MATERIALS AND METHODS: Three different inducible Cre transgenic mice with Ai9-tdTomato reporter expression (PRG4-9, αSMA-9, and AGC-9) were used to label different cell populations in the shoulder. The defect was created surgically in the supraspinatus. The mice were injected with tamoxifen at surgery to label the cells and sacrificed at 1, 2, and 5 weeks postoperatively...
November 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/26721734/osteogenic-potential-of-alpha-smooth-muscle-actin-expressing-muscle-resident-progenitor-cells
#19
Brya G Matthews, Elena Torreggiani, Emilie Roeder, Igor Matic, Danka Grcevic, Ivo Kalajzic
Heterotopic ossification (HO) is a pathological process where bone forms in connective tissues such as skeletal muscle. Previous studies have suggested that muscle-resident non-myogenic mesenchymal progenitors are the likely source of osteoblasts and chondrocytes in HO. However, the previously identified markers of muscle-resident osteoprogenitors label up to half the osteoblasts within heterotopic lesions, suggesting other cell populations are involved. We have identified alpha smooth muscle actin (αSMA) as a marker of osteoprogenitor cells in bone and periodontium, and of osteo-chondro progenitors in the periosteum during fracture healing...
March 2016: Bone
https://www.readbyqxmd.com/read/26321342/effect-of-low-frequency-mechanical-vibration-on-orthodontic-tooth-movement
#20
COMPARATIVE STUDY
Sumit Yadav, Thomas Dobie, Amir Assefnia, Himank Gupta, Zana Kalajzic, Ravindra Nanda
INTRODUCTION: Our objective was to investigate the effect of low-frequency mechanical vibration (LFMV) on the rate of tooth movement, bone volume fraction, tissue density, and the integrity of the periodontal ligament. Our null hypothesis was that there would be no difference in the amount of tooth movement between different values of LFMV. METHODS: Sixty-four male CD1 mice, 12 weeks old, were used for orthodontic tooth movement. The mice were randomly divided into 2 groups: control groups (baseline; no spring + 5 Hz; no spring + 10 Hz; and no spring + 20 Hz) and experimental groups (spring + no vibration; spring + 5 Hz; spring + 10 Hz; and spring + 20 Hz)...
September 2015: American Journal of Orthodontics and Dentofacial Orthopedics
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