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K Sagomonyants, I Kalajzic, P Maye, M Mina
Members of the fibroblast growth factor (FGF) family play essential and important roles in primary and reparative dentinogenesis, with conflicting results regarding their effects on odontoblast differentiation. Our recent studies showed that the effects of FGF2 on cells in odontoblast lineage were stage-specific and depended on the stage of cell maturity. Continuous exposure of pulp cells to FGF2 inhibited odontoblast differentiation, whereas early and limited exposure of pulp cells to FGF2 resulted in marked increases in odontoblast differentiation...
February 1, 2017: Journal of Dental Research
I Vidovic, A Banerjee, R Fatahi, B G Matthews, N A Dyment, I Kalajzic, M Mina
The goal of this study was to examine the contribution of perivascular cells to odontoblasts during the development, growth, and repair of dentin using mouse molars as a model. We used an inducible, Cre-loxP in vivo fate-mapping approach to examine the contributions of the descendants of cells expressing the αSMA-CreERT2 transgene to the odontoblast lineage. In vivo lineage-tracing experiments in molars showed the contribution of αSMA-tdTomato(+) cells to a small number of newly formed odontoblasts during primary dentinogenesis...
November 10, 2016: Journal of Dental Research
Eliane H Dutra, Mara H O' Brien, Alexandro Lima, Zana Kalajzic, Aditya Tadinada, Ravindra Nanda, Sumit Yadav
OBJECTIVES: To evaluate the cellular and matrix effects of botulinum toxin type A (Botox) on mandibular condylar cartilage (MCC) and subchondral bone. MATERIALS AND METHODS: Botox (0.3 unit) was injected into the right masseter of 5-week-old transgenic mice (Col10a1-RFPcherry) at day 1. Left side masseter was used as intra-animal control. The following bone labels were intraperitoneally injected: calcein at day 7, alizarin red at day 14 and calcein at day 21. In addition, EdU was injected 48 and 24 hours before sacrifice...
2016: PloS One
Mildred C Embree, Mo Chen, Serhiy Pylawka, Danielle Kong, George M Iwaoka, Ivo Kalajzic, Hai Yao, Chancheng Shi, Dongming Sun, Tzong-Jen Sheu, David A Koslovsky, Alia Koch, Jeremy J Mao
Tissue regeneration using stem cell-based transplantation faces many hurdles. Alternatively, therapeutically exploiting endogenous stem cells to regenerate injured or diseased tissue may circumvent these challenges. Here we show resident fibrocartilage stem cells (FCSCs) can be used to regenerate and repair cartilage. We identify FCSCs residing within the superficial zone niche in the temporomandibular joint (TMJ) condyle. A single FCSC spontaneously generates a cartilage anlage, remodels into bone and organizes a haematopoietic microenvironment...
October 10, 2016: Nature Communications
Kei Matsumoto, Sandhya Xavier, Jun Chen, Yujiro Kida, Mark Lipphardt, Reina Ikeda, Annie Gevertz, Mario Caviris, Antonis K Hatzopoulos, Ivo Kalajzic, James Dutton, Brian B Ratliff, Hong Zhao, Zbygniew Darzynkiewicz, Stefan Rose-John, Michael S Goligorsky
: : Accumulation of myofibroblasts is a hallmark of renal fibrosis. A significant proportion of myofibroblasts has been reported to originate via endothelial-mesenchymal transition. We initially hypothesized that exposing myofibroblasts to the extract of endothelial progenitor cells (EPCs) could reverse this transition. Indeed, in vitro treatment of transforming growth factor-β1 (TGF-β1)-activated fibroblasts with EPC extract prevented expression of α-smooth muscle actin (α-SMA); however, it did not enhance expression of endothelial markers...
October 5, 2016: Stem Cells Translational Medicine
Emilie Roeder, Brya G Matthews, Ivo Kalajzic
Advancing our understanding of osteoblast biology and differentiation is critical to elucidate the pathological mechanisms responsible for skeletal diseases such as osteoporosis. Histology and histomorphometry, the classical methods to study osteoblast biology, identify osteoblasts based on their location and morphology and ability to mineralize matrix, but do not clearly define their stage of differentiation. Introduction of visual transgenes into the cells of osteoblast lineage has revolutionized the field and resulted in a paradigm shift that allowed for specific identification and isolation of subpopulations within the osteoblast lineage...
November 2016: Bone
M Ikić Matijašević, D Flegar, N Kovačić, V Katavić, T Kelava, A Šućur, S Ivčević, H Cvija, E Lazić Mosler, I Kalajzić, A Marušić, D Grčević
Our study aimed to determine the functional activity of different osteoclast progenitor (OCP) subpopulations and signals important for their migration to bone lesions, causing local and systemic bone resorption during the course of collagen-induced arthritis in C57BL/6 mice. Arthritis was induced with chicken type II collagen (CII), and assessed by clinical scoring and detection of anti-CII antibodies. We observed decreased trabecular bone volume of axial and appendicular skeleton by histomorphometry and micro-computed tomography as well as decreased bone formation and increased bone resorption rate in arthritic mice in vivo...
December 2016: Clinical and Experimental Immunology
Igor Matic, Brya G Matthews, Xi Wang, Nathaniel A Dyment, Daniel L Worthley, David W Rowe, Danka Grcevic, Ivo Kalajzic
The in vivo origin of bone-producing osteoblasts is not fully defined. Skeletal stem cells, a population of mesenchymal stem cells resident in the bone marrow compartment, are thought to act as osteoprogenitors during growth and adulthood. Quiescent bone lining cells (BLCs) have been suggested as a population capable of activation into mature osteoblasts. These cells were defined by location and their morphology and studies addressing their significance have been hampered by their inaccessibility, and lack of markers that would allow for their identification and tracing...
August 10, 2016: Stem Cells
Ryu Yoshida, Farhang Alaee, Felix Dyrna, Mark S Kronenberg, Peter Maye, Ivo Kalajzic, David W Rowe, Augustus D Mazzocca, Nathaniel A Dyment
: Purpose of this study: To elucidate the origin of cell populations that contribute to rotator cuff healing, we developed a mouse surgical model where a full-thickness, central detachment is created in the supraspinatus. MATERIALS AND METHODS: Three different inducible Cre transgenic mice with Ai9-tdTomato reporter expression (PRG4-9, αSMA-9, and AGC-9) were used to label different cell populations in the shoulder. The defect was created surgically in the supraspinatus...
November 2016: Connective Tissue Research
Brya G Matthews, Elena Torreggiani, Emilie Roeder, Igor Matic, Danka Grcevic, Ivo Kalajzic
Heterotopic ossification (HO) is a pathological process where bone forms in connective tissues such as skeletal muscle. Previous studies have suggested that muscle-resident non-myogenic mesenchymal progenitors are the likely source of osteoblasts and chondrocytes in HO. However, the previously identified markers of muscle-resident osteoprogenitors label up to half the osteoblasts within heterotopic lesions, suggesting other cell populations are involved. We have identified alpha smooth muscle actin (αSMA) as a marker of osteoprogenitor cells in bone and periodontium, and of osteo-chondro progenitors in the periosteum during fracture healing...
March 2016: Bone
Sumit Yadav, Thomas Dobie, Amir Assefnia, Himank Gupta, Zana Kalajzic, Ravindra Nanda
INTRODUCTION: Our objective was to investigate the effect of low-frequency mechanical vibration (LFMV) on the rate of tooth movement, bone volume fraction, tissue density, and the integrity of the periodontal ligament. Our null hypothesis was that there would be no difference in the amount of tooth movement between different values of LFMV. METHODS: Sixty-four male CD1 mice, 12 weeks old, were used for orthodontic tooth movement. The mice were randomly divided into 2 groups: control groups (baseline; no spring + 5 Hz; no spring + 10 Hz; and no spring + 20 Hz) and experimental groups (spring + no vibration; spring + 5 Hz; spring + 10 Hz; and spring + 20 Hz)...
September 2015: American Journal of Orthodontics and Dentofacial Orthopedics
K Sagomonyants, I Kalajzic, P Maye, M Mina
Members of the fibroblast growth factor (FGF) family play essential and important roles in primary and reparative dentinogenesis. Although there appears to be a general agreement on the effects of FGF signaling on the proliferation of pulp cells, there are conflicting results regarding its effects on odontoblast differentiation. We recently examined the effects of continuous exposure of dental pulp cells to FGF2 and showed that the effects of FGF2 on differentiation of progenitor cells into odontoblasts were stage specific and dependent on the stage of cell maturity...
November 2015: Journal of Dental Research
B G Matthews, H Roguljic, T Franceschetti, E Roeder, I Matic, I Vidovic, P Joshi, K-Y Kum, I Kalajzic
BACKGROUND AND OBJECTIVE: Cementum and bone are similar mineralized tissues, but cementum accumulates much more slowly than bone, does not have vasculature or innervation and does not undergo remodeling. Despite these differences, there are no well-established markers to distinguish cementoblasts from other mature mineralizing cells such as osteoblasts and odontoblasts. The purpose of this study was to assess differences in gene expression between cementoblasts and osteoblasts using gene profiling of cell populations isolated directly from osteocalcin-green fluorescent protein (OC-GFP) transgenic mice...
June 2016: Journal of Periodontal Research
Mille Kolind, Justin D Bobyn, Brya G Matthews, Kathy Mikulec, Alastair Aiken, David G Little, Ivo Kalajzic, Aaron Schindeler
To better understand the relative contributions of mesenchymal and endothelial progenitor cells to rhBMP-2 induced bone formation, we examined the distribution of lineage-labeled cells in Tie2-Cre:Ai9 and αSMA-creERT2:Col2.3-GFP:Ai9 reporter mice. Established orthopedic models of ectopic bone formation in the hind limb and spine fusion were employed. Tie2-lineage cells were found extensively in the ectopic bone and spine fusion masses, but co-staining was only seen with tartrate-resistant acid phosphatase (TRAP) activity (osteoclasts) and CD31 immunohistochemistry (vascular endothelial cells), and not alkaline phosphatase (AP) activity (osteoblasts)...
December 2015: Bone
C Henríquez, T T Riquelme, D Vera, F Julio-Kalajzić, P Ehrenfeld, J E Melvin, C D Figueroa, J Sarmiento, C A Flores
AIM: Neutrophils are the first cells to arrive at sites of injury. Nevertheless, many inflammatory diseases are characterized by an uncontrolled infiltration and action of these cells. Cell migration depends on volume changes that are governed by ion channel activity, but potassium channels in neutrophil have not been clearly identified. We aim to test whether KCa3.1 participates in neutrophil migration and other relevant functions of the cell. METHODS: Cytometer and confocal measurements to determine changes in cell volume were used...
January 2016: Acta Physiologica
Christopher Murphy, Zana Kalajzic, Taranpreet Chandhoke, Achint Utreja, Ravindra Nanda, Flavio Uribe
OBJECTIVE: To investigate the association between corticision and different force magnitudes with the amount of root resorption. METHODS: Forty-four male Wistar rats (7 week old) were evaluated after an orthodontic spring delivering either 10 or 100 g was placed on the left maxillary first molars to move molars mesially. Experimental rats were divided into four groups, with 11 animals in each group: (1) LF, no corticision and 10 g of orthodontic force; (2) LFC, corticision and 10 g of force; (3) HF, no corticision and 100 g of force; and (4) HFC, corticision and 100 g of force...
January 2016: Angle Orthodontist
Sumit Yadav, Amir Assefnia, Himank Gupta, Meenakshi Vishwanath, Zana Kalajzic, Veerasathpurush Allareddy, Ravindra Nanda
OBJECTIVE: To investigate the effect of low-frequency mechanical vibration (LFMV) on the prevention of relapse after active orthodontic tooth movement, bone volume fraction (BVF), tissue density, and the integrity of periodontal ligament. MATERIALS AND METHODS: Thirty male CD1, 12-week-old mice were used for the study. Mice were randomly divided into three groups: 1. control group, 2. relapse group, and 3. relapse + 30 Hz vibration group. In the control group, first molar was moved mesially for 7 days using nickel-titanium coil spring delivering 10g of force, whereas in relapse and relapse + 30 Hz groups, first molar was moved mesially for 7 days and then orthodontic force was removed and molar was allowed to relapse for 7 days...
February 2016: European Journal of Orthodontics
Michael D Prater, Valérie Petit, I Alasdair Russell, Rajshekhar R Giraddi, Mona Shehata, Suraj Menon, Reiner Schulte, Ivo Kalajzic, Nicola Rath, Michael F Olson, Daniel Metzger, Marisa M Faraldo, Marie-Ange Deugnier, Marina A Glukhova, John Stingl
Contractile myoepithelial cells dominate the basal layer of the mammary epithelium and are considered to be differentiated cells. However, we observe that up to 54% of single basal cells can form colonies when seeded into adherent culture in the presence of agents that disrupt actin-myosin interactions, and on average, 65% of the single-cell-derived basal colonies can repopulate a mammary gland when transplanted in vivo. This indicates that a high proportion of basal myoepithelial cells can give rise to a mammary repopulating unit (MRU)...
October 2014: Nature Cell Biology
Maria Krevvata, Barbara C Silva, John S Manavalan, Marta Galan-Diez, Aruna Kode, Brya Grace Matthews, David Park, Chiyuan A Zhang, Naomi Galili, Thomas L Nickolas, David W Dempster, William Dougall, Julie Teruya-Feldstein, Aris N Economides, Ivo Kalajzic, Azra Raza, Ellin Berman, Siddhartha Mukherjee, Govind Bhagat, Stavroula Kousteni
The bone marrow niche is thought to act as a permissive microenvironment required for emergence or progression of hematologic cancers. We hypothesized that osteoblasts, components of the niche involved in hematopoietic stem cell (HSC) function, influence the fate of leukemic blasts. We show that osteoblast numbers decrease by 55% in myelodysplasia and acute myeloid leukemia patients. Further, genetic depletion of osteoblasts in mouse models of acute leukemia increased circulating blasts and tumor engraftment in the marrow and spleen leading to higher tumor burden and shorter survival...
October 30, 2014: Blood
J Chen, Y Kamiya, I Polur, M Xu, T Choi, Z Kalajzic, H Drissi, S Wadhwa
OBJECTIVE: Temporomandibular joint (TMJ) diseases predominantly afflict women, suggesting a role for female hormones in the disease process. However, little is known about the role of estrogen receptor (ER) signaling in regulating mandibular condylar cartilage growth. Therefore, the goal of this study was to examine the effects of altered estrogen levels on the mandibular condylar cartilage in wild type (WT) and ER beta Knockout (KO) mice. MATERIALS AND METHODS: 21-day-old female WT (n = 37) and ER beta KO mice (n = 36) were either sham operated or ovariectomized, and treated with either placebo or estradiol...
November 2014: Osteoarthritis and Cartilage
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