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https://www.readbyqxmd.com/read/29153087/a-fluorescence-aptasensor-based-on-two-dimensional-sheet-metal-organic-frameworks-for-monitoring-adenosine-triphosphate
#1
Xiao-Man Hai, Nan Li, Ke Wang, Zhi-Qi Zhang, Jing Zhang, Fu-Quan Dang
In the present study, a facile fluorescence aptasensor based on two-dimensional sheet metal-organic frameworks of N,N-bis(2-hydroxyethyl)dithiooxamidato copper(II) (H2dtoaCu) was developed for the sensitive detection of adenosine triphosphate (ATP). The sensing mechanism was based on the noncovalent interaction between FAM-labeled (fluorescein amidite) ATP aptamers and H2dtoaCu. In the absence of ATP, the FAM-labeled aptamer readily adsorbs onto H2dtoaCu, mainly via π-π stacking and hydrogen bond interactions between the nucleotide bases and the H2dtoaCu surface, leading to the reduction of fluorescence intensity of the FAM by photoinduced electron transfer (PET)...
January 15, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29132010/inhibition-of-sonic-hedgehog-signaling-blocks-cell-migration-and-growth-but-induces-apoptosis-via-suppression-of-foxq1-in-natural-killer-t-cell-lymphoma
#2
Pingyi Liu, Lingling Chen
The present study explored the effects of Forkhead box Q1 (FOXQ1) on cell proliferation, cell cycle and apoptosis via the Sonic hedgehog (Shh) pathway in Natural killer/T-cell lymphoma (NKTCL). Quantitative real time-polymerase chain reaction (qRT-PCR) was performed to detect FOXQ1 expression in 117 NKTCL patients and 120 healthy controls. Additionally, FOXQ1 expression in NKTCL cell lines (HANK-1, NK-92, SNK-6, SNT-8 and YT) was determined by western blotting and qRT-PCR. SNK-6 cells were transfected with FOXQ1-shRNA or Shh pathway inhibitor Cyclopamine/recombinant protein Shh...
November 10, 2017: Leukemia Research
https://www.readbyqxmd.com/read/29131354/effect-of-status-epilepticus-on-expression-of-brain-udp-glucuronosyltransferase-1a-in-rats
#3
Yuki Asai, Hatsuna Tanaka, Masayuki Nadai, Miki Katoh
Status epilepticus (SE) involves severe epileptic seizures that cause oxidative stress in the brain. Oxidative stress is known to influence uridine 5'-diposphate-glucuronosyltransferase (UGT) 1A expression. The present study aimed at elucidating the effect of SE on Ugt1a1, Ugt1a6, and Ugt1a7 expression in the rat brain. Kainic acid was used to create an animal model of SE. Sprague-Dawley rats were treated intraperitoneally with 10 mg/kg kainic acid. Ugt1a1 and Ugt1a7 mRNA levels were increased by SE in the cortex and hippocampus (Ugt1a1: 4...
November 13, 2017: Biopharmaceutics & Drug Disposition
https://www.readbyqxmd.com/read/29125289/a-practical-and-high-affinity-fluorescent-probe-for-uridine-diphosphate-glucuronosyltransferase-1a1-a-good-surrogate-for-bilirubin
#4
Xia Lv, Lei Feng, Chun-Zhi Ai, Jie Hou, Ping Wang, Li-Wei Zou, Jie Cheng, Guang-Bo Ge, Jing-Nan Cui, Ling Yang
This study aimed to develop a practical and high-affinity fluorescent probe for uridine diphosphate glucuronosyltransferase 1A1 (UGT1A1), a key conjugative enzyme responsible for the elimination and detoxification of many potentially harmful compounds. Several substrates derived from N-butyl-4-phenyl-1,8-naphthalimide were designed and synthesized on the basis of the substrate preference of UGT1A1 and the principle of photoinduced electron transfer (PET). Following the preliminary screening, substrate 2 was found with a high specificity and high affinity toward UGT1A1, while such biotransformation brought remarkable changes in fluorescence emission...
November 20, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29124970/a-review-on-the-nucleic-acid-constituents-in-mushrooms-nucleobases-nucleosides-and-nucleotides
#5
Chia-Wei Phan, Joon-Keong Wang, Shiau-Chuen Cheah, Murali Naidu, Pamela David, Vikineswary Sabaratnam
Mushrooms have become increasingly important as a reliable food source. They have also been recognized as an important source of bioactive compounds of high nutritional and medicinal values. The nucleobases, nucleosides and nucleotides found in mushrooms play important roles in the regulation of various physiological processes in the human body via the purinergic and/or pyrimidine receptors. Cordycepin, a 3'-deoxyadenosine found in Cordyceps sinensis has received much attention as it possesses many medicinal values including anticancer properties...
November 10, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/29120718/wound-healing-in-rabbit-corneas-after-flapless-refractive-lenticule-extraction-with-a-345%C3%A2-nm-ultraviolet-femtosecond-laser
#6
Christian M Hammer, Corinna Petsch, Jörg Klenke, Katrin Skerl, Christian Wüllner, Christof Donitzky, Friedrich Paulsen, Michael Scholz, Theo Seiler, Friedrich E Kruse, Johannes Menzel-Severing
PURPOSE: To characterize corneal wound healing in a rabbit model after flapless refractive lenticule extraction with a 345 nm ultraviolet femtosecond laser. SETTING: Departments of Ophthalmology and Anatomy II, University of Erlangen-Nürnberg and Wavelight GmbH, Erlangen, Germany. DESIGN: Experimental study. METHODS: Flapless refractive lenticule extraction was performed in 1 eye each of 20 New Zealand white rabbits (-5...
October 2017: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/29120387/alteration-of-vascular-responsiveness-to-uridine-adenosine-tetraphosphate-in-aortas-isolated-from-male-diabetic-otsuka-long-evans-tokushima-fatty-rats-the-involvement-of-prostanoids
#7
Takayuki Matsumoto, Shota Kobayashi, Makoto Ando, Maika Iguchi, Keisuke Takayanagi, Mihoka Kojima, Kumiko Taguchi, Tsuneo Kobayashi
We investigated whether responsiveness to dinucleotide uridine adenosine tetraphosphate (Up₄A) was altered in aortas from type 2 diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) rats compared with those from age-matched control Long-Evans Tokushima Otsuka (LETO) rats at the chronic stage of disease. In OLETF aortas, we observed the following: (1) Up₄A-induced contractions were lower than those in the LETO aortas under basal conditions, (2) slight relaxation occurred due to Up₄A, but this was not observed in phenylephrine-precontracted LETO aortas, (3) acetylcholine-induced relaxation was reduced (vs...
November 9, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29119664/effects-of-body-condition-score-bcs-on-steroid-and-eicosanoid-metabolizing-enzyme-activity-in-various-mare-tissues-during-winter-anoestrus
#8
Mpt Owen, E N Ferjak, C A Cavinder, K J McCarty, K C Yankey, C G Hart, D D Burnett, Ttn Dinh, C O Lemley
The objective of this study was to determine the activity of steroid- and eicosanoid-metabolizing enzymes in horses with varying BCSs. The BCSs of twenty non-pregnant, anoestrous mares were determined prior to euthanasia, and tissue samples were collected from the liver, kidney, adrenal gland, ovary and endometrium. Cytochrome P450 1A (CYP1A), 2C (CYP2C), 3A (CYP3A) and uridine 5'-diphospho-glucuronosyltransferase (UGT) activities were determined using luminogenic substrates. The MIXED procedure of SAS was used to test the effect of BCS on enzyme activity and differences between tissues...
November 8, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29119617/computer-aided-design-of-human-sialyltransferase-inhibitors-of-hst8sia-iii
#9
Christopher Dobie, Andrew P Montgomery, Rémi Szabo, Danielle Skropeta, Haibo Yu
Sialyltransferase (ST) upregulation and the resultant hypersialylation of tumour cell surfaces is an established hallmark of many cancers including lung, breast, ovarian, pancreatic and prostate cancer. The role of ST enzymes in tumour cell growth and metastasis, as well as links to multi-drug resistance, has seen ST inhibition emerge as a target for potential antimetastatic cancer treatments. The most potent of these reported inhibitors are transition-state analogues. Although there are several examples of these in the literature, many have suspected poor pharmacokinetic properties and are not readily synthetically accessible...
November 9, 2017: Journal of Molecular Recognition: JMR
https://www.readbyqxmd.com/read/29116782/inner-sphere-coordination-of-divalent-metal-ion-with-nucleobase-in-catalytic-rna
#10
Xin Liu, Yu Chen, Carol A Fierke
Identification of the function of metal ions and the RNA moieties, particularly nucleobases, that bind metal ions are important questions in RNA catalysis. Here we combine single-atom and abasic substitutions to probe functions of conserved nucleobases in ribonuclease P (RNase P). Structural and biophysical studies of bacterial RNase P propose direct coordination of metal ions by the nucleobases of conserved uridine and guanosine in helix P4 of the RNA subunit (P RNA). To biochemically probe the function of metal ion interactions, we substituted the universally conserved bulged uridine (U51) in the P4 helix of circularly permuted Bacillus subtilis P RNA with 4-thiouridine, 4-deoxyuridine and abasic modifications and G378/379 with 2-aminopurine, N7-deazaguanosine, and 6-thioguanosine...
November 8, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29115431/genetic-diagnosis-and-pathogenic-analysis-of-an-atypical-hereditary-spherocytosis-combined-with-ugt1a1-partial-deficiency-a-case-report
#11
Yan Yi, Xiqiang Dang, Yonggui Li, Chenyu Zhao, Haiyan Tang, Xiaoliu Shi
Patients with combined hereditary spherocytosis (HS) and uridine diphosphate glucuronosyltransferase 1A1 (UGT1A1) deficiency have been reported sporadically. A discrepancy between the level of elevated serum bilirubin concentration and the degree of anemia may suggest the possibility of a coexistence of these conditions. In the present case report, a 20‑year‑old female presented with congenital jaundice and anemia, but did not present with the discrepancy between hyperbilirubinemia and anemia in the patient's childhood, and was not previously diagnosed with either HS or UGT1A1 deficiency...
October 25, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29114966/a-cross-sectional-clinic-based-study-exploring-whether-variants-within-the-glutathione-s-transferase-haptoglobin-and-uridine-5-diphosphoglucuronosyl-transferase-1a1-genes-are-associated-with-inter-individual-phenotypic-variation-in-sickle-cell-anaemia-in-jamaica
#12
Sharon Howell, Kwesi Marshall, Marvin Reid, Norma McFarlane-Anderson, Colin McKenzie
OBJECTIVES: To explore putative associations between specific variants in either the glutathione S-transferase (GST), haptoglobin (HP), or uridine 5'-diphospho-glucuronosyltransferase 1A1 (UGT1A1) genes and clinically important phenotypes in sickle cell anaemia (HbSS). METHODS: Three hundred and seventy-one HbSS participants were recruited from the Sickle Cell Clinic of the Sickle Cell Unit at the University of the West Indies, Kingston, Jamaica. Markers within four GST superfamily genes, the HP gene, and the UGT1A1 gene were analyzed using PCR-based assays...
November 7, 2017: European Journal of Haematology
https://www.readbyqxmd.com/read/29113203/distribution-of-uridine-diphosphate-glucuronosyltransferase-1a-polymorphisms-and-their-role-in-irinotecan-induced-toxicity-in-patients-with-cancer
#13
Yang Wang, Cuihua Yi, Yawei Wang, Hui Li, Bei Li, Dan Wang, Jintong Du, Lian Liu, Xiuwen Wang
Uridine diphosphate glucuronosyltransferase 1A (UGT1A1), which affects irinotecan metabolism, has been associated with severe adverse reactions in patients with cancer treated with irinotecan. However, neither large-scale analysis of the distribution of UGT1A1 polymorphisms, nor standardized assessment of how UGT1A1 polymorphisms affect irinotecan treatment has been performed in China. The aim of the present study was to investigate the distribution of UGT1A1 polymorphisms (*28 and *6) in 2,093 Chinese patients with cancer who were treated with irinotecan from more than 15 hospitals in Shandong, to examine how the coexistence of UGT1A1*6 and UGT1A1*28 alleles may be able to predict toxicities induced by irinotecan in 105 of the patients, and to search for other relevant risk factors...
November 2017: Oncology Letters
https://www.readbyqxmd.com/read/29104783/expression-levels-of-the-uridine-cytidine-kinase-like-1-protein-as-a-novel-prognostic-factor-for-hepatitis-c-virus-associated-hepatocellular-carcinomas
#14
A Buivydiene, V Liakina, J Valantinas, J Norkuniene, E Mockiene, S Jokubauskiene, R Smaliukiene, L Jancoriene, L Kovalevska, E Kashuba
The expression levels of the two novel oncoproteins uridine-cytidine kinase like-1 (UCKL-1) and mitochondrial ribosomal protein S18-2 (MRPS18-2) were assessed in samples of hepatitis C virus (HCV)-associated hepatocellular carcinoma (HCC) using immunohistochemistry. Tissue microarray (TMA) paraffin blocks were prepared from 42 HCC tumor samples with the corresponding peri-tumor tissues and from 11 tissues of a liver with HCV-induced cirrhosis. We found that the UCKL-1 signal in the liver tissues of the peri-tumor zone in the HCC samples was stronger than that in cirrhosis (50 ± 49...
July 2017: Acta Naturae
https://www.readbyqxmd.com/read/29101027/identification-and-characterization-of-udp-mannose-in-human-cell-lines-and-mouse-organs-differential-distribution-across-brain-regions-and-organs
#15
Kazuki Nakajima, Yasuhiko Kizuka, Yoshiki Yamaguchi, Yoshio Hirabayashi, Kazuo Takahashi, Yukio Yuzawa, Naoyuki Taniguchi
Mannosylation in the endoplasmic reticulum is a key process for synthesizing various glycans. Guanosine diphosphate mannose (GDP-Man) and dolichol phosphate-mannose serve as donor substrates for mannosylation in mammals and are used in N-glycosylation, O-mannosylation, C-mannosylation, and the synthesis of glycosylphosphatidylinositol-anchor (GPI-anchor). Here, we report for the first time that low-abundant uridine diphosphate-mannose (UDP-Man), which can serve as potential donor substrate, exists in mammals...
October 31, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29099122/the-intrinsic-basicity-of-the-phosphate-backbone-exceeds-that-of-uracil-and-thymine-residues-protonation-of-the-phosphate-moiety-is-preferred-over-the-nucleobase-for-pdthd-and-purd
#16
R R Wu, L A Hamlow, C C He, Y-W Nei, G Berden, J Oomens, M T Rodgers
The gas-phase conformations of the protonated forms of thymidine-5'-monophosphate and uridine-5'-monophosphate, [pdThd+H](+) and [pUrd+H](+), are investigated by infrared multiple photon dissociation (IRMPD) action spectroscopy and electronic structure calculations. The IRMPD action spectra of [pdThd+H](+) and [pUrd+H](+) are measured over the IR fingerprint and hydrogen-stretching regions using the FELIX free electron laser and an OPO/OPA laser system. Low-energy conformations of [pdThd+H](+) and [pUrd+H](+) and their relative stabilities are computed at the MP2(full)/6-311+G(2d,2p)//B3LYP/6-311+G(d,p) and B3LYP/6-311+G(2d,2p)//B3LYP/6-311+G(d,p) levels of theory...
November 3, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29098099/life-threatening-irinotecan-induced-toxicity-in-an-adult-patient-with-alveolar-rhabdomyosarcoma-the-role-of-a-ugt1a1-polymorphism
#17
Arnaud Jannin, Benjamin Hennart, Antoine Adenis, Bruno Chauffert, Nicolas Penel
Alveolar rhabdomyosarcoma (AR) in adult patients is an exceptional malignancy. Management of AR is based on (neo)adjuvant chemotherapy combining ifosfamide, vincristine, and actinomycin D and local curative-intent surgery/radiotherapy. In cases of relapsing AR, the combination of temozolomide/irinotecan is regarded as a possible option. Here we describe life-threatening long-lasting toxicity related to the 1st cycle of irinotecan-based chemotherapy in a 56-year-old woman suffering from locally advanced and metastatic head and neck AR...
2017: Case Reports in Oncological Medicine
https://www.readbyqxmd.com/read/29096568/preclinical-evaluation-of-safety-of-fucoidan-extracts-from-undaria-pinnatifida-and-fucus-vesiculosus-for-use-in-cancer-treatment
#18
Lata Mathew, Maryam Burney, Anjali Gaikwad, Pranavand Nyshadham, Elizabeth K Nugent, Anneliese Gonzalez, Judith A Smith
OBJECTIVES: To evaluate potential hepatic metabolism-mediated drug interactions with fucoidan from Undaria pinnatifida (UPF) or Fucus vesiculosus (FVF) and potential growth inhibition activity with either fucoidan alone or with chemotherapy. In vivo studies were done to confirm safety and investigate fucoidan-mediated immune modulation. METHODS: Cytochrome P450 (CYP450) 3A4, 2C8, 2C9, and 2D6 inhibition experiments were conducted in vitro followed by an ex vivo human hepatocytes model to evaluate the CYP450 induction potential of each fucoidan at highest theoretical concentrations...
December 2017: Integrative Cancer Therapies
https://www.readbyqxmd.com/read/29090729/tricyclanos-conformationally-constrained-nucleoside-analogues-with-a-new-heterotricycle-obtained-from-a-d-ribofuranose-unit
#19
Máté Kicsák, Attila Mándi, Szabolcs Varga, Mihály Herczeg, Gyula Batta, Attila Bényei, Anikó Borbás, Pál Herczegh
A novel type of nucleoside analogue in which the sugar part is replaced by a new tricycle, 3,7,10-trioxa-11-azatricyclo[5.3.1.0(5,11)]undecane has been prepared by substrate-controlled asymmetric synthesis. 1,5-Dialdehydes obtained from properly protected or unprotected uridine, ribothymidine, cytidine, inosine, adenosine and guanosine by metaperiodate oxidation reacted readily with tris(hydroxymethyl)aminomethane to provide the corresponding tricyclic derivatives with three new stereogenic centers. Through a double cyclisation cascade process the tricyclic compounds were obtained in good to high yields, with very high diastereoselectivity...
November 1, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/29090559/-liver-metabonomics-of-acute-gouty-arthritis-treated-by-diosocorea-nipponica
#20
Shu-Min Liu, Ning Zhang, Dong-Hua Yu, Yu Wang, Qi Zhou, Fang Lu
To explore the prevention and protection effect of Diosocorea nipponica (DNM)) on acute gouty arthritis (AGA) rats based on liver metabonomics, and find potential biomarkers and related pathways. AGA model rats were induced by monosodium urate crystal suspension. UPLC-TOF-MS coupled with pattern recognition technique was employed to find out the potential biomarkers and related metabolic pathways. Eleven common potential biomarkers were identified. Among the potential intervention targets in normal rats given by DNM, 4 biomarkers were up-regulated, and the other 4 targets were down regulated...
May 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
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