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Uridine

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https://www.readbyqxmd.com/read/28103302/crude-extracts-flavokawain-b-and-alpinetin-compounds-from-the-rhizome-of-alpinia-mutica-induce-cell-death-via-uck2-enzyme-inhibition-and-in-turn-reduce-18s-rrna-biosynthesis-in-ht-29-cells
#1
Ibrahim Malami, Ahmad Bustamam Abdul, Rasedee Abdullah, Nur Kartinee Bt Kassim, Rozita Rosli, Swee Keong Yeap, Peter Waziri, Imaobong Christopher Etti, Muhammad Bashir Bello
Uridine-cytidine kinase 2 is an enzyme that is overexpressed in abnormal cell growth and its implication is considered a hallmark of cancer. Due to the selective expression of UCK2 in cancer cells, a selective inhibition of this key enzyme necessitates the discovery of its potential inhibitors for cancer chemotherapy. The present study was carried out to demonstrate the potentials of natural phytochemicals from the rhizome of Alpinia mutica to inhibit UCK2 useful for colorectal cancer. Here, we employed the used of in vitro to investigate the effectiveness of natural UCK2 inhibitors to cause HT-29 cell death...
2017: PloS One
https://www.readbyqxmd.com/read/28097636/andrographolide-antibacterial-activity-against-common-bacteria-of-human-health-concern-and-possible-mechanism-of-action
#2
Malabika Banerjee, Debaprasad Parai, Subrata Chattopadhyay, Samir Kumar Mukherjee
Increasing bacterial resistance to common drugs is a major public health concern for the treatment of infectious diseases. Certain naturally occurring compounds of plant sources have long been reported to possess potential antimicrobial activity. This study was aimed to investigate the antibacterial activity and possible mechanism of action of andrographolide (Andro), a diterpenoid lactone from a traditional medicinal herb Andrographis paniculata. Extent of antibacterial action was assessed by minimal bactericidal concentration method...
January 17, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28088758/c5-substituents-of-uridines-and-2-thiouridines-present-at-the-wobble-position-of-trna-determine-the-formation-of-their-keto-enol-or-zwitterionic-forms-a-factor-important-for-accuracy-of-reading-of-guanosine-at-the-3-end-of-the-mrna-codons
#3
Elzbieta Sochacka, Elzbieta Lodyga-Chruscinska, Justyna Pawlak, Marek Cypryk, Paulina Bartos, Katarzyna Ebenryter-Olbinska, Grazyna Leszczynska, Barbara Nawrot
Modified nucleosides present in the wobble position of the tRNA anticodons regulate protein translation through tuning the reading of mRNA codons. Among 40 of such nucleosides, there are modified uridines containing either a sulfur atom at the C2 position and/or a substituent at the C5 position of the nucleobase ring. It is already evidenced that tRNAs with 2-thiouridines at the wobble position preferentially read NNA codons, while the reading mode of the NNG codons by R5U/R5S2U-containing anticodons is still elusive...
January 13, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28069791/prolonged-indirect-hyperbilirubinemia-in-a-moderately-preterm-boy-with-mediterranean-glucose-6-phosphate-dehydrogenase-and-glutathione-s-transferase-mu-1-null-mutations
#4
Sameer Yaseen Al-Abdi
A 33-week gestation boy with Mediterranean glucose-6-phosphate dehydrogenase (G6PD) and a glutathione S-transferase Mu 1 null mutations (GSTM1*0/*0) developed prolonged indirect hyperbilirubinemia (PIH). He had no laboratory evidence of haemolysis or infection, and no exposure to oxidising agents. He has two full-term older brothers who have no history of neonatal hyperbilirubinemia. One brother, who was exclusively breast fed, has only Mediterranean G6PD and the other has only GSTM1*0/*0. The three boys have no mutation in the uridine diphosphate glucuronosyltransferase 1A1 gene...
January 9, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28068583/no-association-between-non-bullous-skin-reactions-from-lamotrigine-and-heterozygosity-of-ugt1a4-genetic-variants-2-p24t-or-3-l48v-in-norwegian-patients
#5
Maryam Shirzadi, Arne Reimers, Grethe Helde, Wenche Sjursen, Eylert Brodtkorb
PURPOSE: High initial serum concentrations increase the risk of cutaneous adverse reactions. Genetic variants of the main metabolizing isoenzyme, uridine diphosphate glucuronosyltransferase (UGT) 1A4 influence the elimination of lamotrigine (LTG). Our aim was to investigate the potential association between the two best studied variants, *2 (P24T) and *3 (L48V), and the occurrence non-bullous skin reactions from LTG. METHOD: The study included 29 patients of Caucasian ethnicity with a history of non-bullous skin reactions from LTG...
December 22, 2016: Seizure: the Journal of the British Epilepsy Association
https://www.readbyqxmd.com/read/28061332/in%C3%A2-vivo-cleavage-map-illuminates-the-central-role-of-rnase-e-in-coding-and-non-coding-rna-pathways
#6
Yanjie Chao, Lei Li, Dylan Girodat, Konrad U Förstner, Nelly Said, Colin Corcoran, Michał Śmiga, Kai Papenfort, Richard Reinhardt, Hans-Joachim Wieden, Ben F Luisi, Jörg Vogel
Understanding RNA processing and turnover requires knowledge of cleavages by major endoribonucleases within a living cell. We have employed TIER-seq (transiently inactivating an endoribonuclease followed by RNA-seq) to profile cleavage products of the essential endoribonuclease RNase E in Salmonella enterica. A dominating cleavage signature is the location of a uridine two nucleotides downstream in a single-stranded segment, which we rationalize structurally as a key recognition determinant that may favor RNase E catalysis...
January 5, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28058783/characterization-of-phase-i-and-phase-ii-hepatic-metabolism-and-reactive-intermediates-of-larrea-nitida-cav-and-its-lignan-compounds
#7
Hyesoo Jeong, Soolin Kim, Jimin Lee, Jin Young Park, Wenmei Zhou, Xiyuan Liu, So Dam Kim, Yun Seon Song, Chang-Young Jang, Sei-Ryang Oh, Sangho Choi, Minsun Chang
Larrea nitida Cav. (LNC), which belongs to the family Zygophyllaceae, is widely indigenous and used in South America to treat various pathological conditions. It contains the antioxidant and antiinflammatory but toxic nordihydroguaiaretic acid (NDGA) as well as O-methylated metabolite of NDGA (MNDGA) as bioactive compounds. The hepatic metabolism-based toxicological potential of extracts of LNC (LNE), NDGA, and MNDGA has not previously been reported. The present study aimed to characterize the phase I and phase II hepatic metabolism and reactive intermediates of LNE, NDGA, and MNDGA and their effects on the major drug-metabolizing enzymes in vitro and ex vivo...
January 2017: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/28045593/synthesis-and-antibacterial-activity-of-5-tetrachlorophthalimido-and-5-azido-5-deoxyribonucleosides
#8
Robert Van Ostrand, Casey Jacobsen, Alicia Delahunty, Carley Stringer, Ryan Noorbehesht, Haidi Ahmed, Ahmed M Awad
Reported is an efficient synthesis of adenyl and uridyl 5'-tetrachlorophthalimido-5'-deoxyribonucleosides, and guanylyl 5'-azido-5'-deoxyribonucleosides, which are useful in solid-phase synthesis of phosphoramidate and ribonucleic guanidine oligonucleotides. Replacement of 5'-hydroxyl with tetrachlorophthalimido group was performed via Mitsunobu reaction for adenosine and uridine. An alternative method was applied for guanosine which replaced the 5'-hydroxyl with an azido group. The resulting compounds were converted to 5'-amino-5'-deoxyribonucleosides for oligonucleotide synthesis...
January 3, 2017: Nucleosides, Nucleotides & Nucleic Acids
https://www.readbyqxmd.com/read/28045181/mercury-ii-mediated-base-pairs-in-dna-unexpected-behavior-in-metal-ion-binding-and-duplex-stability-induced-by-2-deoxyuridine-5-substituents
#9
Xiurong Guo, Sachin A Ingale, Haozhe Yang, Yang He, Frank Seela
The stability of the mercury ion mediated dU-Hg(II)-dU pair depends on substituents introduced at the 5-position of the pyrimidine moiety. To this end, a series of oligonucleotides were synthesized with dU modification in central position. Common and new phosphoramidites were utilized. Hybridization experiments provided 12-mer duplexes with non-canonical "dU-dU" pairs. In most cases Hg(2+) stabilizes duplexes by metal ion mediated base pair formation identified by higher duplex melting. Among the three types of dU derivatives incorporated in duplex DNA those with small aliphatic side chains have only a minor impact on the stability of the mercury-mediated base pair, while those with a triple bond in the side chain show hysteresis during duplex heating and cooling cycle implying triple bond interaction with mercury ions...
January 3, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28042823/identification-and-analysis-of-rna-editing-sites-in-the-chloroplast-transcripts-of-aegilops-tauschii-l
#10
Mengxing Wang, Hui Liu, Lingqiao Ge, Guangwei Xing, Meng Wang, Song Weining, Xiaojun Nie
RNA editing is an important way to convert cytidine (C) to uridine (U) at specific sites within RNA molecules at a post-transcriptional level in the chloroplasts of higher plants. Although it has been systematically studied in many plants, little is known about RNA editing in the wheat D genome donor Aegilops tauschii L. Here, we investigated the chloroplast RNA editing of Ae. tauschii and compared it with other wheat relatives to trace the evolution of wheat. Through bioinformatics prediction, a total of 34 C-to-U editing sites were identified, 17 of which were validated using RT-PCR product sequencing...
December 30, 2016: Genes
https://www.readbyqxmd.com/read/28041959/substrate-selectivity-of-dengue-and-zika-virus-ns5-polymerase-towards-2-modified-nucleotide-analogues
#11
Supanee Potisopon, François Ferron, Véronique Fattorini, Barbara Selisko, Bruno Canard
In targeting the essential viral RNA-dependent RNA-polymerase (RdRp), nucleotide analogues play a major role in antiviral therapies. In the Flaviviridae family, the hepatitis C virus (HCV) can be eradicated from chronically infected patients using a combination of drugs which generally include the 2'-modified uridine analogue Sofosbuvir, delivered as nucleotide prodrug. Dengue and Zika viruses are emerging flaviviruses whose RdRp is closely related to that of HCV, yet no nucleoside drug has been clinically approved for these acute infections...
December 29, 2016: Antiviral Research
https://www.readbyqxmd.com/read/28039359/comparing-galactan-biosynthesis-in-mycobacterium-tuberculosis-and-corynebacterium-diphtheriae
#12
Darryl A Wesener, Matthew R Levengood, Laura L Kiessling
The suborder Corynebacterineae encompasses species like Corynebacterium glutamicum, which has been harnessed for industrial production of amino acids, as well as Corynebacterium diphtheriae and Mycobacterium tuberculosis, which cause devastating human diseases. A distinctive component of the Corynebacterineae cell envelope is the mycolyl-arabinogalactan (mAG) complex. The mAG is composed of lipid mycolic acids, and arabinofuranose (Araf) and galactofuranose (Galf) carbohydrate residues. Elucidating microbe-specific differences in mAG composition could advance biotechnological applications and lead to new antimicrobial targets...
December 30, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28034895/impaired-aortic-contractility-to-uridine-adenosine-tetraphosphate-in-angiotensin-ii-induced-hypertensive-mice-receptor-desensitization
#13
Zhichao Zhou, Vishal R Yadav, Changyan Sun, Bunyen Teng, Jamal S Mustafa
OBJECTIVE: We previously showed that uridine adenosine tetraphosphate (Up4A)-mediated aortic contraction is partly mediated through purinergic P2X1 receptors (P2X1R). It has been reported that the plasma level of Up4A is elevated in hypertensive patients, implying a potential role for Up4A-P2X1R signaling in hypertension. This study investigated the vasoactive effect of Up4A in aortas isolated from angiotensin (Ang) II-infused (21 days) hypertensive mice. METHODS: Blood pressure was measured by tail cuff plethysmography...
December 29, 2016: American Journal of Hypertension
https://www.readbyqxmd.com/read/28032636/cytosolic-invertase-contributes-to-the-supply-of-substrate-for-cellulose-biosynthesis-in-developing-wood
#14
Umut Rende, Wei Wang, Madhavi Latha Gandla, Leif J Jönsson, Totte Niittylä
Carbon for cellulose biosynthesis is derived from sucrose. Cellulose is synthesized from uridine 5'-diphosphoglucose (UDP-glucose), but the enzyme(s) responsible for the initial sucrose cleavage and the source of UDP-glucose for cellulose biosynthesis in developing wood have not been defined. We investigated the role of CYTOSOLIC INVERTASEs (CINs) during wood formation in hybrid aspen (Populus tremula × tremuloides) and characterized transgenic lines with reduced CIN activity during secondary cell wall biosynthesis...
December 29, 2016: New Phytologist
https://www.readbyqxmd.com/read/28025543/rna-editing-and-its-molecular-mechanism-in-plant-organelles
#15
REVIEW
Mizuho Ichinose, Mamoru Sugita
RNA editing by cytidine (C) to uridine (U) conversions is widespread in plant mitochondria and chloroplasts. In some plant taxa, "reverse" U-to-C editing also occurs. However, to date, no instance of RNA editing has yet been reported in green algae and the complex thalloid liverworts. RNA editing may have evolved in early land plants 450 million years ago. However, in some plant species, including the liverwort, Marchantia polymorpha, editing may have been lost during evolution. Most RNA editing events can restore the evolutionarily conserved amino acid residues in mRNAs or create translation start and stop codons...
December 23, 2016: Genes
https://www.readbyqxmd.com/read/28025164/grilling-enhances-antidiarrheal-activity-of-terminalia-bellerica-roxb-fruits
#16
Garima Pandey, Shyam Sundar Gupta, Anil Bhatia, O P Sidhu, A K S Rawat, Ch V Rao
ETHNOPHARMACOLOGICAL RELEVANCE: Terminalia bellerica Roxb. fruits are rich in a variety of biologically active ingredients. Tharu and Buksa tribes of Udham Singh Nagar, Uttarakhand, India use grilled fruits of Terminalia bellerica as an effective cure for diarrhea. AIM OF THE STUDY: We validated the ethnobotanical claim by comparing the antidiarrheal effect of grilled fruits (GF) with dried fruits (DF). MATERIALS AND METHODS: The 50% ethanolic extracts of GF and DF were successively fractionated; the antioxidant and bacterial inhibition activity were studied using DPPH free radical scavenging, anti-lipid peroxidation and broth dilution method respectively...
December 23, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28018909/a-review-of-selected-genes-with-known-effects-on-performance-and-health-of-cattle
#17
REVIEW
Eduardo Casas, Marcus E Kehrli
There are genetic conditions that influence production in dairy and beef cattle. The objective of this review was to describe relevant genetic conditions that have been associated with productivity and health in cattle. Genes or genomic regions that have been identified as a candidate for the condition will be included, and the genetic basis of the condition will be defined. Genes and genetic conditions included in this review are bovine leukocyte adhesion deficiency, deficiency of the uridine monophosphate synthase, bovine chronic interstitial nephritis, horn development, myostatin, complex vertebral malformation, leptin, osteopetrosis, apoptosis peptide activating factor 1, chondrodysplastic dwarfism, caseins, calpastatin, umbilical hernia, lactoglobulin, citrullinemia, cholesterol deficiency, prions, thyroglobulin, diacylglycerol acyltransferase, syndactyly, maple syrup urine disease, slick hair, Factor XI deficiency, and μ-Calpain...
2016: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/28018587/case-controlled-study-on-indirect-hyperbilirubinemia-in-exclusively-breast-fed-neonates-and-mutations-of-the-bilirubin-uridine-diphosphate-glucuronyl-transferase-gene-1a1
#18
Amal E Mohammed, Eman G Behiry, Akram E El-Sadek, Waleed E Abdulghany, Dalia M Mahmoud, Abdelfattah A Elkholy
OBJECTIVE: This study aims to investigate the association of neonatal indirect hyperbilirubinemia in exclusively breast-fed infants with UGT1A1 (Uridine Diphosphate-Glucuronyl transferase 1A1) polymorphism. METHODS: 50 neonates were classified into 2 groups: 1) 30 full term neonates with indirect hyperbilirubinemia (gestational age (GA) 39.5 ± 1.2 weeks); 2) 20 apparently healthy full-term neonates. Group 1 was further subdivided based on percentage of body weight lost: (A) less than 10%; (B) 10% or more...
January 2017: Annals of Medicine and Surgery
https://www.readbyqxmd.com/read/28011993/putative-orotate-transporter-of-cryptococcus-neoformans-oat1-is-a-member-of-the-ncs1-prt-transporter-super-family-and-its-loss-causes-attenuation-of-virulence
#19
Akio Toh-E, Misako Ohkusu, Kiminori Shimizu, Azusa Takahashi-Nakaguchi, Susumu Kawamoto, Naruhiko Ishiwada, Akira Watanabe, Katsuhiko Kamei
It is well known that 5-fluoroorotic acid (5-FOA)-resistant mutants isolated from wild-type Cryptococcus neoformans are exclusively either ura3 or ura5 mutants. Unexpectedly, many of the 5-FOA-resistant mutants isolated in our selective regime were Ura(+). We identified CNM00460 as the gene responsible for these mutations. Cnm00460 belongs to the nucleobase cation symporter 1/purine-related transporter (NCS1/PRT) super family of fungal transporters, representative members of which are uracil transporter, uridine transporter and allantoin transporter of Saccharomyces cerevisiae...
December 23, 2016: Current Genetics
https://www.readbyqxmd.com/read/28009546/uridine-as-a-new-scavenger-for-synchrotron-based-structural-biology-techniques
#20
Eva Crosas, Albert Castellvi, Isidro Crespo, Daniel Fulla, Fernando Gil-Ortiz, Gustavo Fuertes, Christina S Kamma-Lorger, Marc Malfois, Miguel A G Aranda, Jordi Juanhuix
Macromolecular crystallography (MX) and small-angle X-ray scattering (SAXS) studies on proteins at synchrotron light sources are commonly limited by the structural damage produced by the intense X-ray beam. Several effects, such as aggregation in protein solutions and global and site-specific damage in crystals, reduce the data quality or even introduce artefacts that can result in a biologically misguiding structure. One strategy to reduce these negative effects is the inclusion of an additive in the buffer solution to act as a free radical scavenger...
January 1, 2017: Journal of Synchrotron Radiation
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