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https://www.readbyqxmd.com/read/29666907/three-dimensional-analysis-of-nuclear-heterochromatin-distribution-during-early-development-in-the-rabbit
#1
Amélie Bonnet-Garnier, Kiên Kiêu, Tiphaine Aguirre-Lavin, Krisztina Tar, Pierre Flores, Zichuan Liu, Nathalie Peynot, Martine Chebrout, András Dinnyés, Véronique Duranthon, Nathalie Beaujean
Changes to the spatial organization of specific chromatin domains such as constitutive heterochromatin have been studied extensively in somatic cells. During early embryonic development, drastic epigenetic reprogramming of both the maternal and paternal genomes, followed by chromatin remodeling at the time of embryonic genome activation (EGA), have been observed in the mouse. Very few studies have been performed in other mammalian species (human, bovine, or rabbit) and the data are far from complete. During this work, we studied the three-dimensional organization of pericentromeric regions during the preimplantation period in the rabbit using specific techniques (3D-FISH) and tools (semi-automated image analysis)...
April 18, 2018: Chromosoma
https://www.readbyqxmd.com/read/29666031/measurement-of-left-atrial-volume-by-2d-and-3d-non-contrast-computed-tomography-compared-with-cardiac-magnetic-resonance-imaging
#2
Maise Høigaard Fredgart, Rasmus Carter-Storch, Jacob Eifer Møller, Kristian Altern Øvrehus, Redi Pecini, Jordi Sanchez Dahl, Oke Gerke, Roudyna Ahmad Alturkmany, Axel Brandes, Jes Sanddal Lindholt, Axel Cosmus Pyndt Diederichsen
BACKGROUND: Cardiac magnetic resonance imaging (MRI) is considered the gold standard for assessment of left atrial (LA) volume. We assessed the feasibility of evaluating LA volume using 3D non-contrast computed tomography (NCCT). Furthermore, since manual tracing of LA volume is time consuming, we evaluated the accuracy of the LA area using 2D NCCT imaging for LA volume assessment. METHODS: MRI and NCCT imaging were performed in 69 patients before and one year after aortic valve replacement...
April 10, 2018: Journal of Cardiovascular Computed Tomography
https://www.readbyqxmd.com/read/29665420/distortion-matched-t-1-maps-and-unbiased-t-1-weighted-images-as-anatomical-reference-for-high-resolution-fmri
#3
Wietske van der Zwaag, Pieter F Buur, Alessio Fracasso, Tessa van Doesum, Kamil Uludag, Maarten Versluis, José P Marques
The increasing availability of ultra-high field scanners has led to a growing number of submillimetre fMRI studies in humans, typically targeting the gray matter at different cortical depths. In most analyses, the definition of surfaces at different cortical depths is based on an anatomical image with different contrast and distortions than the functional images. Here, we introduce a novel sequence providing bias-field corrected T1 -weighted images and T1 -maps with distortions that match those of the fMRI data, with an image acquisition time significantly shorter than standard T1 -weighted anatomical imaging...
April 14, 2018: NeuroImage
https://www.readbyqxmd.com/read/29665042/neural-tube-defects-in-embryonic-life-lessons-learned-from-340-early-pregnancy-failures
#4
David R Hartge, Michael Gembicki, Achim Rody, Jan Weichert
OBJECTIVES: The aim of this study was to evaluate the feasibility of sonographic assessment of the embryonic/fetal neural tube in nonviable pregnancies and to determine the defect incidence. METHODS: Prospective analysis of transvaginally acquired 3-dimensional (3D) multiplanar and 3D surface-rendered volume sets of 340 cases of missed abortion between March 2010 and September 2015 was performed. Data regarding karyotype and postmortem examination as well as demographic features and the outcomes of subsequent pregnancies were evaluated...
April 17, 2018: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
https://www.readbyqxmd.com/read/29663370/deformable-torso-phantoms-of-chinese-adults-for-personalized-anatomy-modelling
#5
Hongkai Wang, Xiaobang Sun, Tongning Wu, Congsheng Li, Zhonghua Chen, Meiying Liao, Mengci Li, Wen Yan, Hui Huang, Jia Yang, Ziyu Tan, Libo Hui, Yue Liu, Hang Pan, Yue Qu, Zhaofeng Chen, Liwen Tan, Lijuan Yu, Hongcheng Shi, Li Huo, Yanjun Zhang, Xin Tang, Shaoxiang Zhang, Changjian Liu
In recent years, there has been increasing demand for personalized anatomy modelling for medical and industrial applications, such as ergonomics device development, clinical radiological exposure simulation, biomechanics analysis, and 3D animation character design. In this study, we constructed deformable torso phantoms that can be deformed to match the personal anatomy of Chinese male and female adults. The phantoms were created based on a training set of 79 trunk computed tomography (CT) images (41 males and 38 females) from normal Chinese subjects...
April 16, 2018: Journal of Anatomy
https://www.readbyqxmd.com/read/29660487/monitoring-the-progression-of-erosive-tooth-wear-etw-using-bewe-index-in-casts-and-their-3d-images-a-retrospective-longitudinal-study
#6
Francisca Marro, Liesa De Lat, Luc Martens, Wolfgang Jacquet, Peter Bottenberg
OBJECTIVE: To determine if the Basic erosive tooth wear index (BEWE index) is able to assess and monitor ETW changes in two consecutive cast models, and detect methodological differences when using the corresponding 3D image replicas. METHODS: A total of 480 pre-treatment and 2-year post-treatment orthodontic models (n = 240 cast models and n = 240 3D image replicas) from 120 adolescents treated between 2002-2013 at the Gent Dental Clinic, Belgium, were scored using the BEWE index...
April 13, 2018: Journal of Dentistry
https://www.readbyqxmd.com/read/29655527/the-smiling-scan-technique-facially-driven-guided-surgery-and-prosthetics
#7
Alessandro Pozzi, Lorenzo Arcuri, Peter K Moy
PURPOSE: To introduce a proof of concept technique and new integrated workflow to optimize the functional and esthetic outcome of the implant-supported restorations by means of a 3-dimensional (3D) facially-driven, digital assisted treatment plan. METHODS: The Smiling Scan technique permits the creation of a virtual dental patient (VDP) showing a broad smile under static conditions. The patient is exposed to a cone beam computed tomography scan (CBCT), displaying a broad smile for the duration of the examination...
April 11, 2018: Journal of Prosthodontic Research
https://www.readbyqxmd.com/read/29653353/a-statistical-shape-model-of-the-left-ventricle-from-real-time-3d-echocardiography-and-its-application-to-myocardial-segmentation-of-cardiac-magnetic-resonance-images
#8
M C Carminati, C Piazzese, M Pepi, G Tamborini, P Gripari, G Pontone, R Krause, A Auricchio, R M Lang, E G Caiani
OBJECT: We present in this paper the application of a statistical shape model of the left ventricle (LV) built from transthoracic real time 3D echocardiography (3DE) to segment the LV endocardium and epicardium in cardiac magnetic resonance (CMR) images. MATERIAL AND METHODS: The LV model was built from a training database constituted by over 9000 surfaces obtained from retrospectively selected 3DE examination of 435 patients with various pathologies. Three-dimensional segmentation of the endocardium and the epicardium was obtained by processing CMR images acquired in 30 patients with a dedicated active shape modelling (ASM) algorithm using the proposed LV model...
March 30, 2018: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/29652788/a-novel-lidar-based-instrument-for-high-throughput-3d-measurement-of-morphological-traits-in-maize-and-sorghum
#9
Suresh Thapa, Feiyu Zhu, Harkamal Walia, Hongfeng Yu, Yufeng Ge
Recently, imaged-based approaches have developed rapidly for high-throughput plant phenotyping (HTPP). Imaging reduces a 3D plant into 2D images, which makes the retrieval of plant morphological traits challenging. We developed a novel LiDAR-based phenotyping instrument to generate 3D point clouds of single plants. The instrument combined a LiDAR scanner with a precision rotation stage on which an individual plant was placed. A LabVIEW program was developed to control the scanning and rotation motion, synchronize the measurements from both devices, and capture a 360° view point cloud...
April 13, 2018: Sensors
https://www.readbyqxmd.com/read/29651011/super-resolution-for-asymmetric-resolution-of-fib-sem-3d-imaging-using-ai-with-deep-learning
#10
Katsumi Hagita, Takeshi Higuchi, Hiroshi Jinnai
Scanning electron microscopy equipped with a focused ion beam (FIB-SEM) is a promising three-dimensional (3D) imaging technique for nano- and meso-scale morphologies. In FIB-SEM, the specimen surface is stripped by an ion beam and imaged by an SEM installed orthogonally to the FIB. The lateral resolution is governed by the SEM, while the depth resolution, i.e., the FIB milling direction, is determined by the thickness of the stripped thin layer. In most cases, the lateral resolution is superior to the depth resolution; hence, asymmetric resolution is generated in the 3D image...
April 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29649299/digitization-of-natural-objects-with-micro-ct-and-photographs
#11
Takashi Ijiri, Hideki Todo, Akira Hirabayashi, Kenji Kohiyama, Yoshinori Dobashi
In this paper, we present a three-dimensional (3D) digitization technique for natural objects, such as insects and plants. The key idea is to combine X-ray computed tomography (CT) and photographs to obtain both complicated 3D shapes and surface textures of target specimens. We measure a specimen by using an X-ray CT device and a digital camera to obtain a CT volumetric image (volume) and multiple photographs. We then reconstruct a 3D model by segmenting the CT volume and generate a texture by projecting the photographs onto the model...
2018: PloS One
https://www.readbyqxmd.com/read/29626838/integrated-computer-aided-forensic-case-analysis-presentation-and-documentation-based-on-multimodal-3d-data
#12
Alexander Bornik, Martin Urschler, Dieter Schmalstieg, Horst Bischof, Astrid Krauskopf, Thorsten Schwark, Eva Scheurer, Kathrin Yen
Three-dimensional (3D) crime scene documentation using 3D scanners and medical imaging modalities like computed tomography (CT) and magnetic resonance imaging (MRI) are increasingly applied in forensic casework. Together with digital photography, these modalities enable comprehensive and non-invasive recording of forensically relevant information regarding injuries/pathologies inside the body and on its surface. Furthermore, it is possible to capture traces and items at crime scenes. Such digitally secured evidence has the potential to similarly increase case understanding by forensic experts and non-experts in court...
March 23, 2018: Forensic Science International
https://www.readbyqxmd.com/read/29625202/yeast-inner-subunit-pa-nz-1-labeling-strategy-for-accurate-subunit-identification-in-a-macromolecular-complex-through-cryo-em-analysis
#13
Huping Wang, Wenyu Han, Junichi Takagi, Yao Cong
Cryo-electron microscopy (cryo-EM) has been established as one of the central tools in the structural study of macromolecular complexes. Although intermediate- or low-resolution structural information through negative staining (NS) or cryo-EM analysis remains highly valuable, we lack general and efficient ways to achieve unambiguous subunit identification in these applications. Here, we took advantage of the extremely high affinity between a dodecapeptide "PA" tag and the NZ-1 antibody Fab fragment to develop an efficient "yeast inner-subunit PA-NZ-1 labeling" (YISPANL) strategy that when combined with cryo-EM could precisely identify subunits in macromolecular complexes...
April 3, 2018: Journal of Molecular Biology
https://www.readbyqxmd.com/read/29623539/registration-of-3d-freehand-ultrasound-to-a-bone-model-for-orthopedic-procedures-of-the-forearm
#14
Matija Ciganovic, Firat Ozdemir, Fabien Pean, Philipp Fuernstahl, Christine Tanner, Orcun Goksel
PURPOSE: For guidance of orthopedic surgery, the registration of preoperative images and corresponding surgical plans with the surgical setting can be of great value. Ultrasound (US) is an ideal modality for surgical guidance, as it is non-ionizing, real time, easy to use, and requires minimal (magnetic/radiation) safety limitations. By extracting bone surfaces from 3D freehand US and registering these to preoperative bone models, complementary information from these modalities can be fused and presented in the surgical realm...
April 5, 2018: International Journal of Computer Assisted Radiology and Surgery
https://www.readbyqxmd.com/read/29623419/axial-plane-dissimilarities-of-two-identical-lenke-type-6c-scoliosis-cases-visualized-and-analyzed-by-vertebral-vectors
#15
Tamás S Illés, Máté Burkus, Szabolcs Somoskeőy, Fabien Lauer, Francois Lavaste, Jean F Dubousset
PURPOSE: The global appearance of scoliosis in the horizontal plane is not really known. Therefore, the aims of this study were to analyze scoliosis in the horizontal plane using vertebral vectors in two patients classified with the same Lenke group, and to highlight the importance of the information obtained from these vertebral vector-based top-view images in clinical practice. METHODS: Two identical cases of scoliosis were selected, based on preoperative full-body standing anteroposterior and lateral radiographs obtained by the EOS™ 2D/3D system...
April 6, 2018: European Spine Journal
https://www.readbyqxmd.com/read/29611216/a-semiautomatic-segmentation-method-for-prostate-in-ct-images-using-local-texture-classification-and-statistical-shape-modeling
#16
Maysam Shahedi, Martin Halicek, Rongrong Guo, Guoyi Zhang, David S Schuster, Baowei Fei
PURPOSE: Prostate segmentation in computed tomography (CT) images is useful for treatment planning and procedure guidance such as external beam radiotherapy and brachytherapy. However, because of the low, soft tissue contrast of CT images, manual segmentation of the prostate is a time-consuming task with high interobserver variation. In this study, we proposed a semiautomated, three-dimensional (3D) segmentation for prostate CT images using shape and texture analysis and we evaluated the method against manual reference segmentations...
April 2, 2018: Medical Physics
https://www.readbyqxmd.com/read/29604454/bayesian-convolutional-neural-network-based-mri-brain-extraction-on-nonhuman-primates
#17
Gengyan Zhao, Fang Liu, Jonathan A Oler, Mary E Meyerand, Ned H Kalin, Rasmus M Birn
Brain extraction or skull stripping of magnetic resonance images (MRI) is an essential step in neuroimaging studies, the accuracy of which can severely affect subsequent image processing procedures. Current automatic brain extraction methods demonstrate good results on human brains, but are often far from satisfactory on nonhuman primates, which are a necessary part of neuroscience research. To overcome the challenges of brain extraction in nonhuman primates, we propose a fully-automated brain extraction pipeline combining deep Bayesian convolutional neural network (CNN) and fully connected three-dimensional (3D) conditional random field (CRF)...
March 28, 2018: NeuroImage
https://www.readbyqxmd.com/read/29603966/correlation-between-perception-of-color-shadows-and-surface-textures-and-the-realism-of-a-scene-in-virtual-reality
#18
Pedro J Pardo, María Isabel Suero, Ángel Luis Pérez
Head-mounted displays allow us to go through immersive experiences in virtual reality and are expected to be present in more and more applications in both recreational and professional fields. In this context, recent years have witnessed significant advances in rendering techniques following physical models of lighting and shading. The aim of this paper is to check the fidelity of the visual appearance of real objects captured through a 3D scanner, rendered in a personal computer and displayed in a virtual reality device...
April 1, 2018: Journal of the Optical Society of America. A, Optics, Image Science, and Vision
https://www.readbyqxmd.com/read/29603755/impact-of-left-atrial-size-on-isolation-area-in-the-acute-phase-of-pulmonary-vein-isolation-using-28-mm-cryoballoon
#19
Akio Chikata, Takeshi Kato, Kazuo Usuda, Shuhei Fujita, Michiro Maruyama, Yoshiki Nagata, Satoru Sakagami, Naomi Kanamori, Takanori Yaegashi, Takahiro Saeki, Takashi Kusayama, Soichiro Usui, Hiroshi Furusho, Shuichi Kaneko, Masayuki Takamura
BACKGROUND: The impact of left atrial (LA) size on isolation area (ISA) using a 28-mm second-generation cryoballoon (CB) in the acute phase after pulmonary vein isolation (PVI) and the differences of CB from contact force-guided radiofrequency (RF) ablation have not been fully investigated. METHODS: We examined 85 consecutive patients (CB group, 35; RF group, 50) with drug-refractory paroxysmal atrial fibrillation who underwent their first PVI procedure at two institutions...
March 31, 2018: Pacing and Clinical Electrophysiology: PACE
https://www.readbyqxmd.com/read/29601750/resolving-point-defects-in-the-hydration-structure-of-calcite-10-4-with-three-dimensional-atomic-force-microscopy
#20
Hagen Söngen, Bernhard Reischl, Kazuki Miyata, Ralf Bechstein, Paolo Raiteri, Andrew L Rohl, Julian D Gale, Takeshi Fukuma, Angelika Kühnle
It seems natural to assume that defects at mineral surfaces critically influence interfacial processes such as the dissolution and growth of minerals in water. The experimental verification of this claim, however, is challenging and requires real-space methods with utmost spatial resolution, such as atomic force microscopy (AFM). While defects at mineral-water interfaces have been resolved in 2D AFM images before, the perturbation of the surrounding hydration structure has not yet been analyzed experimentally...
March 16, 2018: Physical Review Letters
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