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https://www.readbyqxmd.com/read/29148897/oxidative-stress-response-in-sh-sy5y-cells-exposed-to-short-term-1800%C3%A2-mhz-radiofrequency-radiation
#1
Ana Marija Marjanovic Cermak, Ivan Pavicic, Ivancica Trosic
The exact mechanism that could explain the effects of radiofrequency (RF) radiation exposure at non-thermal level is still unknown. Increasing evidence suggests a possible involvement of reactive oxygen species (ROS) and development of oxidative stress. To test the proposed hypothesis, human neuroblastoma cells (SH-SY5Y) were exposed to 1800 MHz short-term RF exposure for 10, 30 and 60 minutes. Electric field strength within Gigahertz Transverse Electromagnetic cell (GTEM) was 30 V m(-1) and specific absorption rate (SAR) was calculated to be 1...
November 17, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/29142509/using-an-nmr-metabolomics-approach-to-investigate-the-pathogenicity-of-amyloid-beta-and-alpha-synuclein
#2
M M Phelan, E Caamaño-Gutiérrez, M S Gant, R X Grosman, J Madine
Introduction: The pathogenicity at differing points along the aggregation pathway of many fibril-forming proteins associated with neurodegenerative diseases is unclear. Understanding the effect of different aggregation states of these proteins on cellular processes is essential to enhance understanding of diseases and provide future options for diagnosis and therapeutic intervention. Objectives: To establish a robust method to probe the metabolic changes of neuronal cells and use it to monitor cellular response to challenge with three amyloidogenic proteins associated with neurodegenerative diseases in different aggregation states...
2017: Metabolomics: Official Journal of the Metabolomic Society
https://www.readbyqxmd.com/read/29141366/-in-vitro-early-detection-of-amyloid-plaques-in-alzheimer-s-disease-by-pittsburgh-compound-b-modified-magnetic-nanoparticles
#3
J Q Zeng, J Q Wu, M H Li, P J Wang
Objective: To construct magnetic nanoparticles targeting β-amyloid (Aβ) plaques, the pathological biomarker of Alzheimer's disease (AD) and to study their binding capability in vitro. Methods: Superparamagnetic nanoparticles Mn(0.6)Zn(0.4)Fe(2)O(4) (MZF) were coated with amphiphilic star-block copolymeric micelles and modified with Aβ-specific probe Pittsburgh compound B (PiB) to construct a novel magnetic nanoparticle MZF-PiB, which specifically targeted amyloid plaques. Transmission electron microscope was used to study the morphological features of MZF-PiB...
November 7, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29137252/a-novel-mutation-r190h-in-the-at-hook-1-domain-of-mecp2-identified-in-an-atypical-rett-syndrome
#4
Xiao Zhou, Yuangao Liao, Miaojing Xu, Zhong Ji, Yunqi Xu, Liang Zhou, Xiaoming Wei, Peiqian Hu, Peng Han, Fanghan Yang, Suyue Pan, Yafang Hu
Background: Mutations in Methyl-CpG binding protein 2 (MECP2) have been identified as the disease-causing mutations in Rett Syndrome (RTT). However, no mutation in the AT-hook 1 domain of MECP2 has been reported in RTT yet. The function of AT-hook 1 domain of MECP2 has not been described either. Methods: The clinical and radiological features of a girl with progressive hyperactivity and loss of acquired linguistic and motor functions were presented. Next generation sequencing was used to screen the causative gene...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29134401/duloxetine-protects-human-neuroblastoma-cells-from-oxidative-stress-induced-cell-death-through-akt-nrf-2-ho-1-pathway
#5
Daiane Fátima Engel, Jade de Oliveira, Vicente Lieberknecht, Ana Lucia Severo Rodrigues, Andreza Fabro de Bem, Nelson Horácio Gabilan
The contribution of oxidative stress to the pathophysiology of depression has been described in numerous studies. Particularly, an increased production of reactive oxygen species (ROS) caused by mitochondrial dysfunction can lead to neuronal cell death. Human neuroblastoma SH-SY5Y cells were used to investigate the neuroprotective effect of the antidepressant duloxetine against rotenone-induced oxidative stress. SH-SY5Y cells were pretreated with duloxetine (1-5 µM) for 24 h followed by a 24-h rotenone exposure (10 µM)...
November 13, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/29134085/gene-delivery-ability-of-polyethylenimine-and-polyethylene-glycol-dual-functionalized-nanographene-oxide-in-11-different-cell-lines
#6
Liping Wu, Jinshan Xie, Tan Li, Zihao Mai, Lu Wang, Xiaoping Wang, Tongsheng Chen
We recently developed a polyethylenimine (PEI) and polyethylene glycol (PEG) dual-functionalized reduced graphene oxide (GO) (PEG-nrGO-PEI, RGPP) for high-efficient gene delivery in HepG2 and Hela cell lines. To evaluate the feasibility and applicability of RGPP as a gene delivery carrier, we here assessed the transfection efficiency of RGPP on gene plasmids and siRNA in 11 different cell lines. Commercial polyalkyleneimine cation transfection reagent (TR) was used as comparison. In HepG2 cells, RGPP exhibited much stronger delivery ability for siRNA and large size plasmids than TR...
October 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/29128627/inhibition-of-jak1-by-microrna-708-promotes-sh-sy5y-neuronal-cell-survival-after-oxygen-and-glucose-deprivation-and-reoxygenation
#7
Chao Huang, Haitao Zhou, Xiangyang Ren, Junfang Teng
MicroRNAs mediates gene expression in various diseases. Studies have shown that aberrant expression of miRNAs affected cerebral protection. In this study, we have investigated the effects of microRNA-708 (miR-708) on cell survival of oxygen and glucose-deprived reoxygenation (OGD/R) human neuroblastoma cells (SH-SY5Y) and explored whether miR-708 inhibited neuronal death by targeting JAK1. In vitro model of ischemia was used to investigate the neuroprotective functions of miR-708. MiR-708 mimics/siJAK1 transfected SH-SY5Y cells were treated with OGD...
November 9, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/29128626/neuroprotective-and-neurotrophic-effects-of-lanthionine-ketimine-ester
#8
Natalia Marangoni, Kathy Kowal, Zane Deliu, Kenneth Hensley, Douglas L Feinstein
Lanthionine ketimine ethyl ester (LKE) is a synthetic derivative of the naturally occurring amino acid lanthionine ketimine. We previously showed that LKE reduced clinical signs in a mouse model of multiple sclerosis (MS) associated with reductions in axonal damage; however, whether LKE has direct beneficial actions on mammalian neuronal cells was not examined. In the current study, we tested the effects of LKE in SH-SY5Y human neuronal cells and in primary mouse cerebellar granule neurons. In both cell types, LKE dose-dependently reduced the cell death that occurred spontaneously followed a change in media...
November 8, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/29126968/jnk-signaling-pathway-regulates-sorbitol-induced-tau-proteolysis-and-apoptosis-in-sh-sy5y-cells-by-targeting-caspase-3
#9
Marta Olivera Santa-Catalina, Montaña Caballero Bermejo, Ricardo Argent, Juan C Alonso, Francisco Centeno, María J Lorenzo
Growing evidence suggests that Diabetes Mellitus increases the risk of developing Alzheimer's disease. It is well known that hyperglycemia, a key feature of Diabetes Mellitus, may induce plasma osmolarity disturbances. Both hyperglycemia and hyperosmolarity promote the altered post-translational regulation of microtubule-associated protein Tau. Interestingly, abnormal hyperphosphorylation and cleavage of Tau have been proven to lead to the genesis of filamentous structures referred to as neurofibrillary tangles, the main pathological hallmark of Alzheimer's disease...
November 7, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29125390/responsiveness-of-voltage-gated-calcium-channels-in-sh-sy5y-human-neuroblastoma-cells-on-micropillar-substrates
#10
Wenxu Wang, Donghuo Zhong, Yu Lin, Rong Fan, Zhengjun Hou, Xiumei Cao, Yubin Ren
In this study, poly-L-lactic acid (PLLA) micropillar substrates were fabricated to evaluate the influence of topographic substrates on cell morphological and functional characteristics, such as spreading area, voltage-gated calcium channels (VGCCs) and membrane potential. The proliferation, spreading area, perimeter and circularity of SH-SY5Y cells interfaced with different substrates were first investigated. In addition, the cytoskeleton and focal adhesion of a cell as important manifestations of cell morphology were analyzed by immunofluorescence...
November 10, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/29115830/gastrodin-and-isorhynchophylline-synergistically-inhibit-mpp-induced-oxidative-stress-in-sh-sy5y-cells-by-targeting-erk1-2-and-gsk-3%C3%AE-pathways-involvement-of-nrf2-nuclear-translocation
#11
Qiang Li, Chengu Niu, Xiaojie Zhang, Miaoxian Dong
The pathogenesis of Parkinson's disease (PD) is multifactorial event. Combination therapies might be more effective in controlling the disease. Thus, the studies reported were designed to test the hypothesis that gastrodin (GAS)-induced de novo synthesis of nuclear factor E2-related factor 2 (Nrf2) and isorhynchophylline (IRN) inhibition of Nrf2 nuclear export contribute to their additive or synergistic neuroprotective effect. Here, we have demonstrated that the combination of GAS and IRN (GAS/IRN) protects SH-SY5Y cells against 1-methyl-4-phenylpyridinium (MPP(+)) toxicity in a synergistic manner...
November 17, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/29115502/different-inhibitory-effects-on-the-proliferation-and-apoptosis-of-human-and-laboratory-borna-disease-virus%C3%A2-infected-human-neuroblastoma-sh%C3%A2-sy5y-cells-in%C3%A2-vitro
#12
Hua Huang, Hong Zhang, Dan Li, Shigang Chen, Chanjuan Zhou, Qi Li, Peng He, Liang Fang, Yong Zhang, Xiaomei Li, Jingjing Zhou, Lin Sun, Siwen Liu, Yujie Guo, Ying Huang, Peng Xie
Borna disease virus (BDV) is a neurotropic and non‑cytolytic virus, which causes behavioral disorders in a wide range of warm‑blooded species. It is well established that BDV induces neurodegeneration by impairing neurogenesis and interfering with neuronal functioning in the limbic system. In the present study, the potential role of BDV infection in SH‑SY5Y cells was identified, and comparisons of two original BDV strains (the human Hu‑H1 and the laboratory Strain V) were performed to further elucidate the phenotypes of BDV pathogenesis with strain differences...
November 8, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29115479/overexpression-of-mir%C3%A2-185-inhibits-autophagy-and-apoptosis-of-dopaminergic-neurons-by-regulating-the-ampk-mtor-signaling-pathway-in-parkinson-s-disease
#13
Zhi Wen, Jie Zhang, Peng Tang, Ning Tu, Ke Wang, Guangyao Wu
Parkinson's disease (PD) is an age‑associated neurodegenerative disorder characterized by the death of dopaminergic neurons in the substantia nigra pars compacta. Activation of 5'‑adenosine monophosphate‑activated protein kinase (AMPK) has been suggested to be associated with PD pathogenesis. The aim of the present study was to investigate the effects of the aberrant expression of microRNA‑185 (miR‑185) in PD. A 1‑methyl‑4‑phenyl‑1,2,3,6‑tetrahydropyridine (MPTP)‑induced in vitro model of PD was generated using the human SH‑SY5Y dopaminergic neuroblastoma cell line, in order to examine the potential molecular mechanisms underlying the roles of miR‑185 in PD...
October 26, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29115424/cornin-protects-sh%C3%A2-sy5y-cells-against-oxygen-and-glucose-deprivation%C3%A2-induced-autophagy-through-the-pi3k-akt-mtor-pathway
#14
Changling Ding, Jie Zhang, Baoyuan Li, Zhaoxing Ding, Wenna Cheng, Fei Gao, Ye Zhang, Yangyang Xu, Shuping Zhang
It has been reported that cornin may reduce neuronal death during cerebral ischemia; however, little is known about the molecular mechanism of the role of corninin autophagy in SH‑SY5Y neuronal cells. In the present study, oxygen‑glucose deprivation (OGD)‑treated cells were used as a cerebral ischemia model in vitro. The results demonstrated that cornin was able to reduce neuronal cell loss, increase the apoptosis regulator Bcl‑2/apoptosis regulator BAX ratio, and decrease the protein levels of caspase‑3...
October 25, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29114206/controversial-effects-of-d-amino-acid-oxidase-activator-daoa-g72-on-d-amino-acid-oxidase-dao-activity-in-human-neuronal-astrocyte-and-kidney-cell-lines-the-n-methyl-d-aspartate-nmda-receptor-hypofunction-point-of-view
#15
Vinita Jagannath, Zacharias Faidon Brotzakis, Michele Parrinello, Susanne Walitza, Edna Grünblatt
Dysfunction of D-amino acid oxidase (DAO) and DAO activator (DAOA)/G72 genes have been linked to neuropsychiatric disorders. The glutamate hypothesis of schizophrenia has proposed that increased DAO activity leads to decreased D-serine, which subsequently may lead to N-methyl-D-aspartate (NMDA) receptor hypofunction. It has been shown that DAOA binds to DAO and increases its activity. However, there are also studies showing DAOA decreases DAO activity. Thus, the effect of DAOA on DAO is controversial. We aimed to understand the effect of DAOA on DAO activity in neuron-like (SH-SY5Y), astrocyte-like (1321N1) and kidney-like (HEK293) human cell lines...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/29113281/direct-effects-of-phenformin-on-metabolism-bioenergetics-and-viability-of-sh-sy5y-neuroblastoma-cells
#16
Fintan Geoghegan, Naomi Chadderton, G Jane Farrar, Daniela M Zisterer, Richard K Porter
Phenformin, a member of the biguanides class of drugs, has been reported to be efficacious in cancer treatment. The focus of the current study was to establish whether there were direct effects of phenformin on the metabolism and bioenergetics of neuroblastoma SH-SY5Y cancer cells. Cell viability was assessed using the alamar blue assay, flow cytometry analysis using propidium iodide and annexin V stain and poly (ADP-ribose) polymerase analysis. Cellular and mitochondrial oxygen consumption was determined using a Seahorse Bioscience Flux analyser and an Oroboros Oxygraph respirometer...
November 2017: Oncology Letters
https://www.readbyqxmd.com/read/29111270/4-substituted-sampangine-derivatives-novel-acetylcholinesterase-and-%C3%AE-myloid-aggregation-inhibitors
#17
Ke-Lin Chen, Ling Gan, Zhen-Hua Wu, Jin-Fang Qin, Wen-Xia Liao, Huang Tang
A series of 4- substituted sampangine derivatives (4-aminoalkylaminosampangine Ar-NH(CH2)nNR1R2) has been designed, synthesized, and tested for their ability to inhibit acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and β-myloid (Aβ) aggregation. The synthetic compounds exhibited high AChE inhibitory activity and a significant in vitro inhibitory potency toward the self-induced Aβ aggregation. While, treatment of SH-SY5Y cells overexpressing the Swedish mutant form of human β-amyloid precursor protein (APPsw) with derivatives was associated with significant reduction of Aβ42 secretion levels...
October 27, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29110463/8-nitro-cgmp-attenuates-the-interaction-between-snare-complex-and-complexin-through-s-guanylation-of-snap-25
#18
Yusuke Kishimoto, Kohei Kunieda, Atsushi Kitamura, Yuki Kakihana, Takaaki Akaike, Hideshi Ihara
8-Nitroguanosine 3',5'-cyclic monophosphate (8-nitro-cGMP) is the second messenger in nitric oxide/reactive oxygen species redox signaling. This molecule covalently binds to protein thiol groups, called S-guanylation, and exerts various biological functions. Recently, we have identified synaptosomal-associated protein 25 (SNAP-25) as a target of S-guanylation, and demonstrated that S-guanylation of SNAP25 enhanced SNARE complex formation. In this study, we have examined the effects of S-guanylation of SNAP-25 on the interaction between the soluble SNARE complex and complexin (cplx), which binds to the SNARE complex with a high affinity...
November 7, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/29110208/expression-of-gas1-in-mouse-brain-release-and-role-in-neuronal-differentiation
#19
Elizabeth Bautista, Natanael Zarco, Nicolás Aguirre-Pineda, Manuel Lara-Lozano, Paula Vergara, Juan Antonio González-Barrios, Raúl Aguilar-Roblero, José Segovia
Growth arrest-specific 1 (Gas1) is a pleiotropic protein that induces apoptosis of tumor cells and has important roles during development. Recently, the presence of two forms of Gas1 was reported: one attached to the cell membrane by a GPI anchor; and a soluble extracellular form shed by cells. Previously, we showed that Gas1 is expressed in different areas of the adult mouse CNS. Here, we report the levels of Gas1 mRNA protein in different regions and analyzed its expressions in glutamatergic, GABAergic, and dopaminergic neurons...
November 6, 2017: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/29110181/c-myb-plays-an-essential-role-in-the-protective-function-of-igf-1-on-cytotoxicity-induced-by-a%C3%AE-25-35-via-the-pi3k-akt-pathway
#20
Jingyu Zhang, Yongwei Shu, Yang Qu, Lina Zhang, Tingting Chu, Yonghui Zheng, Hong Zhao
There have been numerous reports about neurodegenerative diseases, including Alzheimer's disease. Nevertheless, the molecules responsible for the neurodegeneration in Alzheimer's disease are basically unknown. Recent findings indicate that the cellular myeloblastosis (c-myb) regulates neural progenitor cell proliferation. In the current study, the function of insulin-like growth factor-1 (IGF-1) against cell toxicity in SH-SY5Y cells induced by β-amyloid 25-35 (Aβ25-35) and its molecular mechanism were investigated...
November 6, 2017: Journal of Molecular Neuroscience: MN
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