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salivary amylase

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https://www.readbyqxmd.com/read/28811748/salivary-diagnostic-markers-in-males-and-females-during-rest-and-exercise
#1
Kay Rutherfurd-Markwick, Carlene Starck, Deborah K Dulson, Ajmol Ali
BACKGROUND: Saliva is a useful diagnostic tool for analysis in sports, exercise and nutrition research, as collection is easy and non-invasive and it contains a large number of analytes affected by a range of physiological and pathological stressors and conditions. This study examined key salivary electrolytes and stress and immune markers in males and females at rest and during exercise. METHODS: Unstimulated whole saliva from 20 healthy, recreationally active participants (8 males and 12 females) was analysed for flow rate, osmolality, sodium (Na(+)), potassium (K(+)), chloride (Cl(-)), secretory immunoglobulin A (SIgA), α-amylase activity and cortisol during both rest and moderate intensity (70% peak power) cycling exercise in a randomised crossover design...
2017: Journal of the International Society of Sports Nutrition
https://www.readbyqxmd.com/read/28802982/reducing-of-salivary-%C3%AE-amylase-inhibition-by-using-bovine-serum-albumin-and-calcium-chloride-for-forensic-saliva-screening
#2
Jun Ohta, Masako Ohmura
Inhibiting salivary α-amylase is a critical issue of forensic saliva identification using the catalytic method. This study aims to identify human α-amylase inhibitors in forensic saliva screening by using a blue starch amylase test and to measure the extent of enzyme inhibition. Thus, in order to demonstrate the presence of inhibitors, we prepared positively charged metal ion sources or chelators that were mixed into the saliva stains. The results of this study show that ferric chloride (FeCl3), magnesium chloride, ethylenediaminetetraacetic acid, and citric acid significantly decrease the α-amylase activity of saliva stains...
July 25, 2017: Legal Medicine
https://www.readbyqxmd.com/read/28800355/work-related-stress-and-role-of-personality-in-a-sample-of-italian-bus-drivers
#3
Margherita Bergomi, Alberto Modenese, Enrica Ferretti, Angela Ferrari, Giuseppe Licitra, Roberto Vivoli, Fabriziomaria Gobba, Gabriella Aggazzotti
BACKGROUND: Several studies have shown that professional drivers are at risk of developing work-related stress. Stress may be responsible for a variety of adverse effects and may also be associated with an increased number of accidents. OBJECTIVE: Perform an integrated, objective and subjective evaluation of work-related stress in bus drivers, that also considered the role of personality traits. METHODS: Salivary α-amylase and cortisol were measured in 42 bus drivers...
2017: Work: a Journal of Prevention, Assessment, and Rehabilitation
https://www.readbyqxmd.com/read/28783453/development-of-human-salivary-gland-like-tissue-in-vitro
#4
Marc Burghartz, Simon Lennartz, Matthias Schweinlin, Rudolf Hagen, Norbert Kleinsasser, Stephan Hackenberg, Gudrun Steußloff, Agmal Scherzad, Kathrin Radeloff, Christian Ginzkey, Heike Walles, Marco Metzger
The loss of salivary gland function caused by radiation therapy of the head and neck is a serious condition and it affects a patient's quality of life. The current lack of effective therapies demands new options to be explored. This study tested whether human salivary gland epithelial cells (SGECs) could be successfully cultured on a decellularized porcine gut matrix (SIS-muc) in both mono- and coculture with microvascular endothelial cells (mvECs). By performing immunofluorescence imaging, transmission as well as scanning electron microscopy (SEM), quantitative polymerase chain reaction (qPCR), and an amylase enzyme assay, it was investigated as to what extent the three-dimensional (3D)-cultured cells could maintain their molecular differentiation and the production of working α-amylase (α-AMY) compared with two-dimensional (2D) culture...
August 4, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28775392/optimisation-of-octinyl-succinic-anhydride-starch-stablised-w1-o-w2-emulsions-for-oral-destablisation-of-encapsulated-salt-and-enhanced-saltiness
#5
Natalie Chiu, Amparo Tarrega, Christopher Parmenter, Louise Hewson, Bettina Wolf, Ian D Fisk
Sodium (salt) was encapsulated within the inner water phase of w1/o/w2 food emulsions externally stabilised by starch particles with the ultimate aim of enhancing saltiness perception. The physical properties of the starch particles were modified by octenyl succinic anhydride (OSA) treatment (0-3%) to vary the degree of hydrophobicity of the emulsifying starch. During oral processing native salivary amylase hydrolysed the starch and destabilised the o/w emulsion releasing the inner w/o phase and subsequently sodium into the oral cavity, resulting in a salty taste...
August 2017: Food Hydrocolloids
https://www.readbyqxmd.com/read/28766747/impact-of-salivary-enzyme-activity-on-the-oral-perception-of-starch-containing-foods
#6
John Bridges, John Smythe, Raven Reddrick
It has long been held that product developers should not rely solely on instrumental measures of texture. This study examined the widely accepted effects of salivary α amylase on mouth thinning during oral processing. To understand this phenomenon, 13 descriptive panelists were trained to manipulate starch thickened semisolid foods and note when changes in the perceived thickness occurred. The panelists were subsequently grouped based on their reports of how quickly these foods broke down in their oral cavity...
August 2017: Journal of Texture Studies
https://www.readbyqxmd.com/read/28766112/oleanolic-acid-and-ursolic-acid-as-potential-inhibitors-of-human-salivary-%C3%AE-amylase-insights-from-in-vitro-assays-and-in-silico-simulations
#7
Jiachen Sun, Shengjie Dong, Yueting Wu, Hui Zhao, Xia Li, Wenyuan Gao
It is known that inhibiting α-amylase, an important enzyme in digestion of starch and glycogen, is a useful strategy for treating disorders in carbohydrate uptake. Two natural components distributed in many fruits and plants, oleanolic acid and ursolic acid, are endowed with important pharmacological activities and wide therapeutic possibilities. Until now, only a tiny fraction of their applications have been identified and exploited. Our in vitro inhibition studies demonstrated that oleanolic acid and ursolic acid non-competitively inhibit the activity and function of human salivary α-amylase...
August 2017: Journal of Molecular Modeling
https://www.readbyqxmd.com/read/28753943/reduced-stress-and-improved-sleep-quality-caused-by-green-tea-are-associated-with-a-reduced-caffeine-content
#8
Keiko Unno, Shigenori Noda, Yohei Kawasaki, Hiroshi Yamada, Akio Morita, Kazuaki Iguchi, Yoriyuki Nakamura
Caffeine, one of the main components in green tea, can interfere with sleep and block the effect of theanine. Since theanine, the main amino acid in tea leaves, has significant anti-stress effects in animals and humans, we examined the effects of green tea with lowered caffeine content, i.e., low-caffeine green tea (LCGT), on stress and quality of sleep of middle-aged individuals (n = 20, mean age 51.3 ± 6.7 years) in a double-blind crossover design. Standard green tea (SGT) was used as the control. These teas (≥300 mL/day), which were eluted with room temperature water, were consumed over a period of seven days after a single washout term...
July 19, 2017: Nutrients
https://www.readbyqxmd.com/read/28750755/evening-salivary-cortisol-and-alpha-amylase-at-14months-and-neurodevelopment-at-4years-sex-differences
#9
Ainara Andiarena, Nekane Balluerka, Mario Murcia, Jesús Ibarluzea, Vivette Glover, Oscar Vegas
Stress system activity in early life can have long-term effects on neurodevelopment. The main aim of this study was to assess the association of child evening salivary cortisol and alpha-amylase basal levels at 14months of age with longer-term neuropsychological development at 4years in a low-risk population-based birth cohort derived from the INMA (Environment and Childhood) project in Spain. We included 186 parent-children pairs with information on both stress system activity and neurodevelopment. Both stress markers at 14months of age showed an association with neuropsychological development at 4years...
July 26, 2017: Hormones and Behavior
https://www.readbyqxmd.com/read/28738617/flexible-immunosensor-for-the-detection-of-salivary-%C3%AE-amylase-in-body-fluids
#10
Bartolomeo Della Ventura, Nikola Sakač, Riccardo Funari, Raffaele Velotta
The development of a portable testing device for detecting Human Salivary α-Amylase (HSA) is very timely since such an enzyme is a valuable biomarker for diagnosing many diseases and monitoring the human stress. We show that an easy-to-use and robust device like the Quartz-Crystal Microbalance (QCM) can be a suitable platform for HSA sensing with a limit of detection of 1µg/mL (77 U/L). The functionalization of the gold surface is realized by the Photochemical Immobilization Technique (PIT), a powerful and simple method based on an appropriate UV-activation of antibodies...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28735172/exposure-to-intimate-partner-violence-in-utero-and-infant-internalizing-behaviors-moderation-by-salivary-cortisol-alpha-amylase-asymmetry
#11
Cecilia Martinez-Torteya, G Anne Bogat, Joseph S Lonstein, Douglas A Granger, Alytia A Levendosky
Guided by the main tenets of contemporary models of the developmental origins of health and disease, this study evaluated whether individual differences in reactivity of the hypothalamic-pituitary-adrenal (HPA) axis and Sympathetic Nervous System (SNS) moderate the effect of prenatal exposure to trauma on internalizing and externalizing behaviors during infancy. Participants were a community sample of 182 mothers (M age=25years, 43% Caucasian, 33% Black/African American, 24% Biracial/Other) and their infants (59% girls; M age=11...
July 20, 2017: Early Human Development
https://www.readbyqxmd.com/read/28716529/the-pro-inflammatory-cytokines-salivary-cortisol-and-alpha-amylase-are-associated-with-generalized-anxiety-disorder-gad-in-patients-with-asthma
#12
Chang-Jiang Yang, Di Liu, Zhen-Sheng Xu, Shen-Xun Shi, Yi-Jie Du
Generalized anxiety disorder (GAD) is common in patients with asthma. High levels of GAD may lead both to exacerbation of the condition and poor management. However, the physiological mechanisms of GAD in asthma patient is unclear. This study investigated the associations between the diurnal rhythm of sputum cytokines, salivary cortisol, α-amylase and GAD in asthma patients. Patients with co-morbid GAD and asthma showed higher sputum IL-1 AUC, sputum IL-6 AUC and sAA AUC. And there were positive correlations between Hamilton anxiety scale (HAMA) scores and sputum IL-1 AUC concentrations (r=0...
July 14, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28711894/-markers-of-dental-children-s-health-in-the-application-of-therapeutic-orthodontic-equipment
#13
Аліса В Пачевська, Аліна В Білошицька
INTRODUCTION: Treatment of teeth anomalies using removable and non-removable orthodontic devices in children leads to complications such as caries, gingivitis, periodontitis, oral mucosa hyperplasia. Etiopathogenetical of these diseases can be associated with biochemical changes in the composition of saliva. AIM: To determine the activity of lysozyme and amylase in oral fluid in children when using a fixed and removable orthodontic devices. MATERIALS AND METHODS: Amylase and lysozyme were studied in oral fluid...
2017: Wiadomości Lekarskie: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/28700470/salivary-alpha-amylase-salivary-cortisol-and-anxiety-during-a-youth-taekwondo-championship-an-observational-study
#14
Laura Capranica, Giancarlo Condello, Francesco Tornello, Teresa Iona, Salvatore Chiodo, Anna Valenzano, Mario De Rosas, Giovanni Messina, Antonio Tessitore, Giuseppe Cibelli
The aim of this study was to assess the stress-related responses and the coach's capability to match perceived efforts of youth athletes during a taekwondo championship.Using a cross-sectional study design, salivary cortisol (sC) and alpha-amylase (sAA) were measured in 6 males and 3 females young (11.0 ± 0.9 years) athletes at awakening, 5 minutes before, and 1 minute and 30 minutes after official combats. State anxiety was recorded 60 minutes before the first competition, whereas coach's and athletes' ratings of perceived exertion (RPE) were obtained at the end of the combats...
July 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28693593/psychological-stress-is-related-to-a-decrease-of-serum-anti-m%C3%A3-llerian-hormone-level-in-infertile-women
#15
Yue-Zhi Dong, Fei-Jing Zhou, Ying-Pu Sun
BACKGROUND: Stress exposure has been proved to be linked to reproductive failure. The reproductive potential of women depends on the ovarian reserve. Anti-müllerian hormone (AMH) has been proved a reliable clinical marker of ovarian reserve. However, the correlation between psychological stress and AMH level is not clear. METHODS: A cross-sectional study including 576 women was conducted. AMH concentration was tested to reflect the ovarian reserve. Salivary alpha-amylase (SAA) level was measured to assess the stress of patients objectively...
July 11, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28692340/salivary-cortisol-and-alpha-amylase-is-there-consistency-between-psychosocial-stress-test-and-burdensome-work-shifts
#16
Kati Karhula, Mikko Härmä, Mikael Sallinen, Harri Lindholm, Ari Hirvonen, Marko Elovainio, Mika Kivimäki, Jussi Vahtera, Sampsa Puttonen
This study examined the consistency of salivary cortisol and alpha-amylase (sAA) total daily secretion between laboratory and field circumstances. The 95 participants were shift working female health care professionals with high (n = 53) or low (n = 42) psychosocial stress (job strain) measured by the Job Content Questionnaire (JCQ). The Trier Social Stress Test including a 5-minute free speech and a mental arithmetic task was conducted with four, and field measurements with three daily saliva samples of cortisol and sAA during circadian rhythm and inter-shift recovery controlled morning shift, night shift, and a day off...
July 10, 2017: Journal of Occupational and Environmental Hygiene
https://www.readbyqxmd.com/read/28682138/denatonium-and-naringenin-promote-sca-9-tumor-growth-and-angiogenesis-participation-of-arginase
#17
Ganna Dmytrenko, María E Castro, María E Sales
Submandibular gland (SMG) is one of the major salivary glands, and is formed by acinar cells that are conveyed to the oral cavity by a duct system. We had previously reported that T2R receptors that were originally identified in gustatory tissues were also present in murine SMG. The addition of bitter compounds to the gland reduced nitric oxide production and downregulated amylase secretion. In this work, we investigated the effect of two different bitter compounds namely denatonium and naringenin on tumor progression as well as the presence of T2R in SCA-9 cells derived from a murine tumor induced in SMG...
July 2017: Nutrition and Cancer
https://www.readbyqxmd.com/read/28678755/radiation-therapy-and-serum-salivary-amylase-in-head-and-neck-cancer
#18
REVIEW
Francesca De Felice, Mario Tombolini, Angela Musella, Francesco Marampon, Vincenzo Tombolini, Daniela Musio
Radiation therapy (RT) is a valid treatment option for head and neck cancer (HNC). The risk of RT-induced toxicities is significant, especially due to extended treatment fields. The raise in amylase activity is strictly dependent on the volume of salivary glands included in the irradiated target volume and it is firmly related to the dose. The aim of this review is to report the effects on salivary amylase activity after radiation exposure of salivary glands, in patients with HNC.
June 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28677946/effects-of-probiotics-supplementation-and-circuit-training-on-immune-responses-among-sedentary-young-males
#19
Nur S Ibrahim, Foong K Ooi, Chee K Chen, Ayu S Muhamad
BACKGROUND: Growing evidence suggests that probiotics may have positive benefits on immune responses following endurance exercise. However, little attention has been given to its possible beneficial effects on immune responses following resistance exercise. METHODS: Forty-one healthy sedentary males were recruited and randomised into four groups: sedentary control with placebo (C), probiotics (P), circuit training with placebo (Ex), and circuit training with probiotics (PEx) groups...
July 5, 2017: Journal of Sports Medicine and Physical Fitness
https://www.readbyqxmd.com/read/28674598/cortisol-and-alpha-amylase-changes-during-an-ultra-running-event
#20
Whitney P Deneen, Alexis B Jones
Elevated stress hormone concentrations can positively affect an athlete's overall performance during a competition, and in many cases, are necessary to be able to perform exercise. During extreme exercise, the body's ability to utilize energy efficiently can affect an athlete's performance. Elevated hormonal concentrations can have many benefits in regards to an athlete's overall performance during a competition. The purpose of this study was to examine the effects of long distance running, such as seen during an ultra-running event (distances beyond 26...
2017: International Journal of Exercise Science
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