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Aila Coello, Antonio Pellicer, Ana Cobo
In recent years, growing evidence for the safety and efficiency of oocyte vitrification has made this technique be increasingly proposed for fertility preservation (FP). The populations who could benefit from FP include oncological patients who need the option to preserve their gametes before undergoing potential sterilizing treatment, patients with non oncologic conditions requiring gonadotoxic chemotherapy and women who wish to delay their motherhood for a variety of reasons. By vitrifying oocytes, women have the chance to conceive in the future, have their own genetic offspring and maintain their reproductive autonomy...
March 8, 2018: Minerva Ginecologica
Jaime Guerrero, Miguel Gallardo, Adoración Rodríguez-Arnedo, Jorgen Ten, Rafael Bernabeu
The survival of human blastocysts to vitrification with two different carriers is compared. Both vitrification carriers used in this study are in the category of closed carriers, as they completely isolate the samples from direct contact with liquid nitrogen or its vapours during cooling and storage, until warming. This characteristic is appealing because it reduces or eliminates the theoretical risk of cross-contamination during that period of time. The two closed vitrification systems used present very different design and features: in the High Security Vitrification device, the carrier straw containing the embryos is encapsulated inside an external straw before plunging in liquid nitrogen, resulting in thermal insulation during cooling...
March 8, 2018: Cryobiology
C C Marques, C Santos-Silva, C Rodrigues, J E Matos, T Moura, M C Baptista, A E M Horta, R J B Bessa, S P Alves, G Soveral, R M L N Pereira
The cryopreservation process must be improved to enhance oocyte cryosurvival and functionality. Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated. In both protocols, calcium (Ca2+ ) free or -containing base media were tested. Oocytes were subjected to vitrification or only exposed to CPAs without immersion in liquid nitrogen. Oocyte's viability, cortical granules location and competence for development after fertilization were assessed...
March 7, 2018: Cryobiology
Willem Stock, Eveline Pinseel, Sam De Decker, Josefin Sefbom, Lander Blommaert, Olga Chepurnova, Koen Sabbe, Wim Vyverman
Diatoms constitute the most diverse group of microalgae and have long been recognised for their large biotechnological potential. In the wake of growing research interest in new model species and development of commercial applications, there is a pressing need for long-term preservation of diatom strains. While cryopreservation using dimethylsulfoxide (DMSO) as a cryoprotective agent is the preferred method for long-term strain preservation, many diatom species cannot be successfully cryopreserved using DMSO...
March 9, 2018: Scientific Reports
Ariadna Corral, Macarena Clavero, Miguel Gallardo, Marcin Balcerzyk, Christiani A Amorim, Ángel Parrado-Gallego, Marie-Madeleine Dolmans, Fernanda Paulini, John Morris, Ramón Risco
Ovarian tissue cryopreservation is, in most cases, the only fertility preservation option available for female patients soon to undergo gonadotoxic treatment. To date, cryopreservation of ovarian tissue has been carried out by both traditional slow freezing method and vitrification, but even with the best techniques, there is still a considerable loss of follicle viability. In this report, we investigated a stepped cryopreservation procedure which combines features of slow cooling and vitrification (hereafter called stepped vitrification)...
March 5, 2018: Cryobiology
Geng An, Zihao Zou, Ryan Flannigan, Jianqiao Liu, Hongzi Du, Xin Fu, Feixiang Guo, Wen Zhang
BACKGROUND As a safety and efficacy protocol, oocyte vitrification has been widely used in IVF treatment. The aim of this study was to evaluate the outcome of ICSI-ET utilizing vitrified oocytes with sperm obtained from non-obstructive azoospermia (NOA) patients via micro-TESE. MATERIAL AND METHODS A total of 150 NOA patients underwent micro-TESE. Ten patients were unable to ejaculate and refused to accept TESA at the time of oocyte retrieval; later, these patients underwent TESA. A total of 174 obstructive azoospermia (OA) patients underwent TESA...
March 7, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Christine Decanter, Geoffroy Robin, Audrey Mailliez, Julien Sigala, Franck Morschhauser, Nassima Ramdane, Patrick Devos, Didier Dewailly, Brigitte Leroy-Martin, Laura Keller
A lower number of metaphase II oocytes eligible for vitrification after controlled stimulation in cancer patients has recently been reported, suggesting that cancer may impair the dynamics and quality of follicular growth. In this prospective, non-interventional study, the pattern of follicular growth and oocyte cohort after ovarian stimulation in cancer patients was analysed. Ninety cancer patients, recruited before starting chemotherapy, were compared with 180 time- and age-matched healthy controls undergoing intracytoplasmic sperm injection...
February 19, 2018: Reproductive Biomedicine Online
V V Yuzhakov, I V Malinova, M V Kiseleva, N K Fomina, L N Bandurko, E V Komarova, L E Sevan'kaeva, I E Ingel', N D Yakovleva, A D Kaprin
The results of a complex morphofunctional study with markers of proliferation (PCNA and Ki-67), angiogenesis (CD31 and CD34), and structural integrity of mesenchymal cells (vimentin) suggest that the pool of primordial follicles was well preserved in vitrified ovarian tissue and that viability of its cellular components can be rapidly restored during incubation at 37°C within 4 h after thawing.
March 5, 2018: Bulletin of Experimental Biology and Medicine
I I Katkov, V F Bolyukh, G T Sukhikh
Kinetic (dynamic) vitrification is a promising trend in cryopreservation of biological materials because it allows avoiding the formation of lethal intracellular ice and minimizes harmful effects of highly toxic penetrating cryoprotectants. A uniform cooling protocol and the same instruments can be used for practically all types of cells. In modern technologies, the rate of cooling is essentially limited by the Leidenfrost effect. We describe a novel platform for kinetic vitrification of biological materials KrioBlastTM that realizes hyper-fast cooling and allows overcoming the Leidenfrost effect...
March 4, 2018: Bulletin of Experimental Biology and Medicine
Giuseppe Scherillo, Valerio Loianno, Davide Pierleoni, Rosario Esposito, Antonio Brasiello, Matteo Minelli, Ferruccio Doghieri, Giuseppe Mensitieri
Atactic polystyrene, as reported in a recent contribution by our group, displays a marked change in glass transition when exposed to toluene vapor due to plasticization associated to vapor sorption within the polymer. The dependence of glass transition temperature of the polymer-penetrant mixture on the pressure of toluene vapor is characterized by the so called 'retrograde vitrification' phenomenon, in that, at a constant pressure, a rubber to glass transition occurs by increasing the temperature. In this contribution, we have used a theoretical approach, based on non-random lattice fluid thermodynamic model for the polymer-toluene mixture, to predict the state of this system, i...
March 2, 2018: Journal of Physical Chemistry. B
Jan Rozsypal, Martin Moos, Petr Šimek, Vladimír Koštál
Some insects rely on the strategy of freeze tolerance for winter survival. During freezing, extracellular body water transitions from the liquid to solid phase and cells undergo freeze-induced dehydration. Here we present results of a thermal analysis (from differential scanning calorimetry) of ice fraction dynamics during gradual cooling after inoculative freezing in variously acclimated larvae of two drosophilid flies, Drosophila melanogaster and Chymomyza costata. Although the species and variants ranged broadly between 0 and close to 100% survival of freezing, there were relatively small differences in ice fraction dynamics...
March 1, 2018: Journal of Experimental Biology
J L P R Jivago, F Paulini, R C Silva, M S Araujo, A P S Marinho, C M Lucci
The aim of this study was to define the population, morphological and ultrastructural characteristics of bitch preantral follicles (PAFs) and to compare the effects on the morphology of PAF of two cryopreservation techniques - slow freezing (SF) and vitrification (V) - of bitches' ovarian tissue. The average population (number per ovary) of PAFs was 48,541 ± 18,366, where 94.25% were primordial (45,145 ± 16,076). The average diameter of the primordial follicles was 27.5 ± 4.2 μm. The overall percentage of morphologically normal PAFs was 93...
February 23, 2018: Cryobiology
Benjamin P Jones, Srdjan Saso, Anastasia Mania, J Richard Smith, Paul Serhal, Jara Ben Nagi
Given the age-related decline in ovarian reserve and oocyte quality, it is unsurprising the global trend of deferring motherhood has resulted in increased levels of involuntary childlessness. The development of oocyte vitrification, with pregnancy and livebirth rates now comparable to using fresh oocytes, has provided an opportunity to electively cryopreserve oocytes for future use, empowering women with the capacity to delay their childbearing years. Whilst it enhances reproductive autonomy, age-related obstetric complications, economic implications and the risk of unsuccessful future treatment make it a controversial therapeutic option...
February 26, 2018: Acta Obstetricia et Gynecologica Scandinavica
A Nohalez, C A Martinez, I Parrilla, J Roca, M A Gil, H Rodriguez-Martinez, E A Martinez, C Cuello
In this study, the effects of addition of the antioxidant ascorbic acid (AsA) were evaluated during porcine in vitro embryo production (IVP) and vitrification. In experiment 1, the effects of AsA supplementation during IVM, IVF and IVC were evaluated, using a total of 2744 oocytes in six replicates. The IVM, IVF and embryo IVC media were supplemented or not (control) with 50 μg/mL AsA in all possible combinations. No significant effects of AsA were detected in any of the maturation, fertilization or embryo development parameters assessed...
February 16, 2018: Theriogenology
Yongshun Ma, Bo Pan, Haoxuan Yang, Izhar Hyder Qazi, Zhenzheng Wu, Changjun Zeng, Ming Zhang, Qingyong Meng, Guangbin Zhou
The present study aimed to investigate the effect of vitrification on the expression of fertilization related genes (CD9 and CD81) and DNA methyl transferases (DNMT1 and DNMT3b) in bovine germinal vesicle (GV) oocytes and their resulting metaphase Ⅱ (MⅡ) stages after in vitro maturation culture. GV oocytes were vitrified using the open-pulled straw method; after warming, they were cultured in vitro. The vitrified-warmed GV oocytes and more developed MII oocytes were used to calculate the maturation rates (first polar body extrusion under a stereomicroscopy), and to detect mRNA expression (qRT-PCR)...
February 21, 2018: Cryobiology
Gang Liu, Shubin Li, Haihan Yuan, Mengrui Hao, Wurihan, Zhizhong Yun, Jie Zhao, Yuzhen Ma, Yanfeng Dai
The survival rate of vitrified-thawed ovarian tissues after autotransplantation still needs to be improved. Therefore finding an ideal cryoprectant to reduce the damage to ovaries that caused by vitrification will pave the way for application of ovary cryopreservation on clinics. Experiments were conducted to investigate the effect of sodium alginate in cryoprotectant solution on mouse ovaries during the vitrification process. The ovaries obtained from 6-weeks old CD1 were assigned into six groups from A to F...
February 9, 2018: Theriogenology
Damià Castelló, Ana Cobo, Enric Mestres, Maria Garcia, Ivette Vanrell, José Alejandro Remohí, Gloria Calderón, Nuno Costa-Borges
Vitrification is currently a well-established technique for the cryopreservation of oocytes and embryos. It can be achieved either by direct (open systems) or indirect (closed systems) contact with liquid nitrogen. While there is not a direct evidence of disease transmission by transferred cryopreserved embryos, it was experimentally demonstrated that cross-contamination between liquid nitrogen and embryos may occur, and thus, the use of closed devices has been recommended to avoid the risk of contamination...
February 20, 2018: Cryobiology
Rebecca D Sandlin, Keith H K Wong, Shannon N Tessier, Anisa Swei, Lauren D Bookstaver, Bennett E Ahearn, Shyamala Maheswaran, Daniel A Haber, Shannon L Stott, Mehmet Toner
Emerging technologies have enabled the isolation and characterization of rare circulating tumor cells (CTCs) from the blood of metastatic cancer patients. CTCs represent a non-invasive opportunity to gain information regarding the primary tumor and recent reports suggest CTCs have value as an indicator of disease status. CTCs are fragile and difficult to expand in vitro, so typically molecular characterization must be performed immediately following isolation. To ease experimental timelines and enable biobanking, cryopreservation methods are needed...
2018: PloS One
Jorge Luis Araújo Martins, Maria Denise Lopes, Fabiana Ferreira de Souza, Fabio Sossai Possebon, Gudrun Wibbelt, Katarina Jewgenow
The aim of this study was to investigate the impact of prolonged storage at 4 °C on survival of cat preantral follicles (PAFs) pre- and post-vitrification. Ovaries were obtained from 12 queens and transported at 4 ºC within 2-6 h. Parts of the ovaries were stored for an additional 24 h or 72 h. The ovarian cortex was dissected, analyzed for viability (neutral red - NR) and morphology (histology - HE and ultrastructural analysis by TEM) and vitrified. We used 2 mm biopsy punches to obtain equal size pieces as the experimental units...
February 19, 2018: Cryobiology
Colin R Gregson, Gregory P Horne, Robin M Orr, Simon M Pimblott, Howard E Sims, Robin J Taylor, Kevin J Webb
The yield of molecular hydrogen, as a function of nitric acid concentration, from the α-radiolysis of aerated nitric acid and its mixtures with sulfuric acid containing plutonium or americium has been investigated. Comparison of experimental measurements with predictions of a Monte Carlo radiation track chemistry model shows that, in addition to scavenging of the hydrated electron, its precursor, and the hydrogen atom, the quenching of excited state water is important in controlling the yield of molecular hydrogen...
February 22, 2018: Journal of Physical Chemistry. B
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