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J Aizpurua, L Medrano, M Enciso, J Sarasa, A Romero, M A Fernández, M J Gómez-Torres
STUDY QUESTION: Is permeable cryoprotectant-free vitrification of native sperm samples a good alternative to conventional slow freezing? SUMMARY ANSWER: The permeable cryoprotectant-free sperm vitrification protocol tested in this study renders considerably better recovery rates of good quality sperm compared to slow freezing. WHAT IS KNOWN ALREADY: Slow freezing is currently the most commonly used technique for sperm cryopreservation, though this method has been repeatedly shown to have negative effects on both structural and functional sperm features...
October 1, 2017: Human Reproduction
Rafael Cuevas-Uribe, E Hu, Harry Daniels, Adriane O Gill, Terrence R Tiersch
The Southern Flounder Paralichthys lethostigma is a high-value species and a promising aquaculture candidate. Because sperm volume can be limited in this species (<500 μL), new sperm cryopreservation methods need to be evaluated. Vitrification is an alternative to conventional slow-rate freezing, whereby small volumes are cryopreserved at high cooling rates (>1,000°C/min). The goal of this work was to develop a standardized approach for vitrification of Southern Flounder sperm. The specific objectives were to (1) evaluate thawing methods and vitrification solutions, (2) evaluate the postthaw membrane integrity of sperm vitrified in different cryoprotectant solutions, (3) examine the relationship between membrane integrity and motility, and (4) evaluate the ability of vitrified sperm to fertilize eggs...
2017: North American Journal of Aquaculture
Núria Arcarons, Roser Morató, Meritxell Vendrell, Marc Yeste, Manel López-Bejar, Kosala Rajapaksha, Muhammad Anzar, Teresa Mogas
This study examines whether incorporating cholesterol-loaded methyl-β-cyclodextrin (CLC) in the bovine oocyte plasma membrane improves oocyte tolerance to vitrification. In vitro matured oocytes were incubated with 2 mg/ml BODIPY-labeled CLC for different time intervals in FCS or PVA supplemented medium or exposed to different CLC concentrations to examine the subcellular localization of cholesterol by confocal microscopy live-cell imaging. Subsequently, the effects of optimized CLC concentrations and incubation times prior to vitrification on early embryo development were assessed...
2017: PloS One
Daniel C Joaquim, Eduardo D Borges, Iara G R Viana, Paula A Navarro, Alessandra A Vireque
The introduction and widespread application of vitrification are one of the most important achievements in human assisted reproduction techniques (ART) of the past decade despite controversy and unclarified issues, mostly related to concerns about disease transmission. Guidance documents published by US Food and Drug Administration, which focused on the safety of tissue/organ donations during Zika virus spread in 2016, as well as some reports of virus, bacteria, and fungi survival to cryogenic temperatures, highlighted the need for a review of the way how potentially infectious material is handled and stored in ART-related procedures...
2017: BioMed Research International
Na Wang, Hai-Sheng Hao, Chong-Yang Li, Ya-Han Zhao, Hao-Yu Wang, Chang-Liang Yan, Wei-Hua Du, Dong Wang, Yan Liu, Yun-Wei Pang, Hua-Bin Zhu, Xue-Ming Zhao
Vitrification reduces the fertilisation capacity and developmental ability of mammalian oocytes; this effect is closely associated with an abnormal increase of cytoplasmic free calcium ions ([Ca(2+)]i). However, little information about the mechanism by which vitrification increases [Ca(2+)]i levels or a procedure to regulate [Ca(2+)]i levels in these oocytes is available. Vitrified bovine oocytes were used to analyse the effect of vitrification on [Ca(2+)]i, endoplasmic reticulum Ca(2+) (ER Ca(2+)), and mitochondrial Ca(2+) (mCa(2+)) levels...
September 6, 2017: Scientific Reports
Takahiro Yamanaka, Teppei Goto, Masumi Hirabayashi, Shinichi Hochi
The practical requirements of islet transplantation necessitate that a large quantity of pancreatic islets be cryopreserved for a long period of time in a simple and convenient manner. We cryopreserved rat islets (size range 101-150 μm in mean diameter) by vitrification with either a Cryotop(®) device or a ø = 57-μm nylon mesh device in units of 10 islets, or by conventional freezing with a Bicell(®) vessel in units of 50 islets. Postwarm/thaw survival rates of the islets were 68.1% ± 5.9%, 64...
September 5, 2017: Biopreservation and Biobanking
Sara Taherzadeh, Mohammad Ali Khalili, Azam Agha-Rahimi, Fateme Anbari, Shahin Ghazali, Guido Macchiarelli
BACKGROUND: Sperm vitrification is a technique of ice and cryoprotectant free cryopreservation by direct plunging of sperm suspension into liquid nitrogen (LN2). The aim of this study was to investigate the influence of cryoprotectant free-vitrification on human sperm fine structure by MSOME technology and the fertility potential by zona binding assay (ZBA). METHODS: 20 normo-ejaculates were prepared by swim up technique, and supernatants were divided into two parts of fresh and vitrified groups...
April 2017: Journal of Reproduction & Infertility
Ana Cobo, Aila Coello, Jose Remohí, Jose Serrano, Jose Maria de Los Santos, Marcos Meseguer
OBJECTIVE: To analyze whether oocyte vitrification may affect subsequent embryo development from a morphokinetic standpoint by means of time-lapse imaging. DESIGN: Observational cohort study. SETTING: University-affiliated private IVF center. PATIENT(S): Ovum donation cycles conducted with the use of vitrified (n = 631 cycles; n = 3,794 embryos) or fresh oocytes (n = 1,359 cycles; n = 9,935 embryos) over 2 years. INTERVENTIONS(S): None...
September 2017: Fertility and Sterility
Mónica H Vazquez-Levin
No abstract text is available yet for this article.
September 2017: Fertility and Sterility
Aila Coello, Marcos Meseguer, Arancha Galán, Lucia Alegre, José Remohí, Ana Cobo
OBJECTIVE: To describe the morphological dynamics of vitrified/warmed blastocysts and to identify quantitative morphological variables related to implantation. Subsequently, by using the most predictive parameters, to develop a hierarchical model by subdividing vitrified/warmed blastocysts into categories with different implantation potentials. DESIGN: Observational, retrospective, cohort study. SETTING: University-affiliated private IVF center...
August 29, 2017: Fertility and Sterility
Hai-Hua Gao, Jun-Tao Li, Jing-Jing Liu, Qing-Ai Yang, Jian-Min Zhang
OBJECTIVE: The aim of this study is to determine the role of autophagy in the immature oocytes during vitrification-warming and in vitro maturation (IVM); the correlations among autophagy, apoptosis, and the activities of caspase in the immature oocytes during vitrification-warming and IVM were also explored. STUDY DESIGN: Immature oocytes from mice were vitrified-warmed and IVM. An autophagy inhibitor (3-methyladenine) was supplemented in cryopreservation solutions and warming solutions...
August 24, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
Rachel Weinerman, Teri Ord, Marisa S Bartolomei, Christos Coutifaris, Monica Mainigi
Epidemiological studies suggest that babies born following in vitro fertilization (IVF) and fresh embryo transfer are of lower birthweight than babies born following frozen embryo transfer, although the mechanism responsible for this phenotype is not known. We developed a novel mouse model that isolates the independent effects of embryo freezing and the superovulated environment, which cannot be performed in humans. We transferred blastocysts that had been vitrified and warmed, mixed with with fresh blastocysts, into individual pseudopregnant recipients produced by either natural mating or mating following injection with equine chorionic gonadotropin and human chorionic gonadotropin and hCG (superovulation)...
July 1, 2017: Biology of Reproduction
Janaína de Souza Guedes, Jhenifer Kliemchen Rodrigues, Ana Luisa Menezes Campos, Camila Cruz de Moraes, João Pedro Junqueira Caetano, Ricardo Mello Marinho
Purpose The present study aimed to evaluate the impact of vitrification on the viability of follicles using a three-dimensional (3D) in vitro culture. Methods Bovine ovarian tissue samples (n = 5) obtained from slaughterhouses were utilized. The cortex was cut into small fragments of 2 × 3 × 0.5 mm using a tissue slicer. From these fragments, secondary follicles were first isolated by mechanical and enzymatic methods, then encapsulated in alginate gel and individually cultured for 20 days...
August 31, 2017: Revista Brasileira de Ginecologia e Obstetrícia
Theesit Juanpanich, Tayita Suttirojpattana, Mari Takayama, Yuanyuan Liang, Osamu Dochi, Rangsun Parnpai, Kei Imai
Generating techniques to enhance the success of blastomere separation is important for bovine economy, because it increases the number of transferable embryos. This study aimed to identify the optimum cryoprotectants for the vitrification of bovine embryos and the separation of blastomeres at different stages. In experiment 1, expanded blastocysts were vitrified in two different vitrification solutions, either (1) ethylene glycol (EG) + propylene glycol (PG) or (2) EG. The survival rate of blastocysts in the EG + PG was higher than that of the EG...
August 30, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
Takayuki Haino, Wataru Tarumi, Kazuhiro Kawamura, Tohru Harada, Kouhei Sugimoto, Aikou Okamoto, Masahiro Ikegami, Nao Suzuki
PURPOSE: To determine the optimal follicle localization for ovarian vitrification in adolescent and young adult (AYA)-aged (between 15 and 39 years of age) patients with cancer or primary ovarian insufficiency (POI). METHODS: In total, ovaries from 24 women were included in our study. These include women who received ovariectomy for fertility preservation before gonadotoxic treatments for cancer (n = 4), or for the treatment of POI by the in vitro activation method (n = 8), and other women and infants (0-3 years of age) whose ovaries were autopsied (n = 12)...
August 28, 2017: Journal of Adolescent and Young Adult Oncology
Moacir R M Radaelli, Carlos G Almodin, Vânia C Minguetti-Câmara, Paula Motta Almodin Cerialli, Aissar E Nassif, Antonio J Gonçalves
OBJECTIVE: This study aimed to compare a new vitrification protocol with reduced cryoprotectant exposure to the slow freezing method in the cryopreservation of prepubertal rat testicular tissue. METHODS: Five sexually immature male Wistar rats were submitted to bilateral orchiectomy. Tissue samples from each testicle were fragmented into small pieces and randomly assigned to three groups: Group A, fresh tissue (control); Group B, slow programmable freezing (SPF); and Group C (vitrification)...
September 1, 2017: JBRA Assisted Reproduction
Mahboobeh Amoushahi, Mojdeh Salehnia, Seyed Javad Mowla, Nassim Ghorbanmehr
OBJECTIVE: This study aimed to evaluate the expression of the genes related to folliculogenesis after vitrification of mouse ovarian tissues using a two-step in vitro culture. MATERIALS AND METHODS: In this experimental study, vitrified and non-vitrified ovaries from 7- day old (neonate) female mice were cultured using alpha-Minimum Essential Medium (α-MEM) supplemented with 5% fetal bovine serum (FBS) for 7 days. Morphology, surface area of ovaries and percentage of normal follicles were evaluated and compared in both groups...
October 2017: Cell Journal
Shogo Higaki, Natsue Kuwata, Kotaro Tanaka, Ikuo Tooyama, Yasuhiro Fujioka, Noriyoshi Sakai, Tatsuyuki Takada
Sperm cryopreservation is a valuable conservation method for endangered fish species. Here we report an easy and efficient cryopreservation method for juvenile whole testis by vitrification and successful sperm production from the vitrified whole testis via in vitro spermatogenesis in the critically endangered cyprinid honmoroko (Gnathopogon caerulescens). Juvenile testis (approximately 10 mm in length and 1 mm in width), consisting predominantly of spermatogonia, were aseptically dissected out and adherent fatty and non-testicular tissues were subsequently removed...
August 24, 2017: Zygote: the Biology of Gametes and Early Embryos
J M Morrell, I Mayer
Many biotechnologies are currently used in livestock breeding with the aim of improving reproductive efficiency and increasing the rate of genetic progress in production animals. Semen cryopreservation is the most widely used cryobiotechnology, although vitrification techniques now allow embryos and oocytes to be banked in ever-increasing numbers. Cryopreservation of other types of germplasm (reproductive tissue in general) is also possible, although the techniques are still in the early stages of development for use in livestock species...
August 24, 2017: Zygote: the Biology of Gametes and Early Embryos
Anne A Y Guilbert, Mohamed Zbiri, Alan D F Dunbar, Jenny Nelson
The optoelectronic properties of blends of conjugated polymers and small molecules are likely to be affected by the molecular dynamics of the active layer components. We study the dynamics of regio-regular poly(3-hexylthiophene) (P3HT): phenyl-C61-butyric acid methyl ester (PCBM) blends using molecular dynamics (MD) simulation on time scales up to 50 ns and in a temperature range of 250-360K. First, we compare the MD results with quasi-elastic neutron scattering (QENS) measurements. Experiment and simulation give evidence of the vitrification of P3HT upon blending, and the plasticization of PCBM by P3HT...
August 23, 2017: Journal of Physical Chemistry. B
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