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Vitrification

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https://www.readbyqxmd.com/read/28546898/treatment-of-fly-ash-from-power-plants-using-thermal-plasma
#1
Sulaiman Al-Mayman, Ibrahim AlShunaifi, Abdullah Albeladi, Imed Ghiloufi, Saud Binjuwair
Fly ash from power plants is very toxic because it contains heavy metals. In this study fly ash was treated with a thermal plasma. Before their treatment, the fly ash was analyzed by many technics such as X-ray fluorescence, CHN elemental analysis, inductively coupled plasma atomic emission spectroscopy and scanning electron microscopy. With these technics, the composition, the chemical and physical proprieties of fly ash are determined. The results obtained by these analysis show that fly ash is mainly composed of carbon, and it contains also sulfur and metals such as V, Ca, Mg, Na, Fe, Ni, and Rh...
2017: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/28545999/a-comparative-analysis-of-the-efficacy-of-three-cryopreservation-protocols-on-the-survival-of-in-vitro-derived-cattle-embryos-at-pronuclear-and-blastocyst-stages
#2
Van Huong Do, Simon Walton, Sally Catt, Andrew W Taylor-Robinson
The effectiveness of three cryopreservation protocols (slow freezing, short equilibration vitrification and long equilibration vitrification) on in vitro-derived cattle embryos at expanded blastocyst and pronuclear stages was compared. 199 expanded blastocysts of good quality were assigned randomly into four treatment groups [control, non-cryopreserved (fresh, unfrozen); and the three cryopreservation methods]. The re-expansion of the cryopreserved blastocysts after 24 h in vitro culture was similar to that of the fresh control group...
May 22, 2017: Cryobiology
https://www.readbyqxmd.com/read/28543267/trehalose-sustains-a-higher-post-thaw-sperm-motility-than-sucrose-in-vitrified-human-sperm
#3
M Schulz, J Risopatrón, G Matus, E Pineda, C Rojas, V Isachenko, E Isachenko, R Sánchez
One of the cryopreservation methods that best preserves sperm function is vitrification. However, comparative studies have not been performed to evaluate the effect of nonpermeable cryoprotectors on sperm function for prolonged periods of time post-devitrification. These times are necessary, especially in in vitro fertilisation and intrauterine insemination, for gamete interaction and then fertilisation to occur, while maintaining motility to arrive at the fertilisation site. In this study, sucrose (.25 m) and trehalose (...
May 25, 2017: Andrologia
https://www.readbyqxmd.com/read/28534059/cryo-mesh-a-simple-alternative-cryopreservation-protocol
#4
B Funnekotter, E Bunn, R L Mancera
BACKGROUND: The continued development of new cryopreservation protocols has improved post-cryogenic success rates for a wide variety of plant species. Methods like the cryo-plate have proven beneficial in simplifying the cryopreservation procedure. OBJECTIVE: This study assessed the practicality of a stainless steel mesh strip (cryo-mesh) for cryopreserving shoot tips from Anigozanthos viridis. MATERIALS AND METHODS: Shoot tips of A. viridis (Kangaroo Paw) were precultured on 0...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28534056/carbon-nanomaterials-enhance-survival-of-agapanthus-praecox-callus-after-cryopreservation-by-vitrification
#5
S M Chen, L Ren, D Zhang, Y F Zhang, X H Shen
BACKGROUND: Adding exogenous compounds is an effective way to improve cell survival after cryopreservation. Carbon nanomaterials (CNMs) are novel exogenous substances with small particle size and good biocompatibility. OBJECTIVE: In this work, four types of CNMs were used for the cryopreservation of Agapanthus praecox callus and their possible effects and mechanism of action were analyzed. MATERIALS AND METHODS: The thermal properties of the vitrification solutions tested were detected by differential scanning calorimetry...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28534054/the-presence-of-zinc-in-the-mouse-ovary-vitrification-medium-histological-evaluation-and-follicle-growth
#6
S Geravandi, M Azadbakht
BACKGROUND: Cryopreservation of ovary is a relevant option for preserving fertility of cancer patients undergoing chemotherapy. OBJECTIVE: To evaluate the effect of zinc in the vitrification medium on histology and follicle growth. MATERIALS AND METHODS: Mouse ovaries were vitrified in vitrification medium supplemented with 0, 100, 150 or 200 μg/dl zinc, identified as V0, V1, V2 and V3 groups, respectively. Histological evaluation of ovaries was carried out...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28534050/cryopreservation-of-lilium-martagon-l-meristems-by-droplet-vitrification-and-evaluation-of-their-physiological-stability
#7
M Urbaniec-Kiepura, A Bach
BACKGROUND: The aim of the present study was to compare different strategies for cryopreservation of martagon lily meristems and to evaluate the physiological status of the regenerants. MATERIALS AND METHODS: The bulblets were stored at 5 degree C or 20 degree C and pretreated with 3% or 6% sucrose prior to droplet-vitrification. The meristems were then assessed for their survival and regeneration. Their photochemical activity was investigated using a Photosynthesis Yield Analyzer MINI PAM 2000 Portable Chlorophyll Fluorometer and their photosynthesis oxygen production was evaluated with a Plant Vital 5030 device...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28531193/raman-microscopy-investigation-of-vitrification-induced-structural-damages-in-mature-bovine-oocytes
#8
Giulia Rusciano, Carolina De Canditiis, Gianluigi Zito, Marcello Rubessa, Maria Serena Roca, Rosa Carotenuto, Antonio Sasso, Bianca Gasparrini
Although oocyte cryopreservation has great potentials in the field of reproductive technologies, it still is an open challenge in the majority of domestic animals and little is known on the biochemical transformation induced by this process in the different cellular compartments. Raman micro-spectroscopy allows the non-invasive evaluation of the molecular composition of cells, based on the inelastic scattering of laser photons by vibrating molecules. The aim of this work was to assess the biochemical modifications of both the zona pellucida and cytoplasm of vitrified/warmed in vitro matured bovine oocytes at different post-warming times...
2017: PloS One
https://www.readbyqxmd.com/read/28527625/cosmetic-micromanipulation-of-vitrified-warmed-cleavage-stage-embryos-does-not-improve-art-outcomes-an-ultrastructural-study-of-fragments
#9
Somayyeh Safari, Mohammad Ali Khalili, Zeinab Barekati, Iman Halvaei, Morteza Anvari, Stefania A Nottola
The aim was to study the ultrastructure of cytoplasmic fragments along with the effect of cosmetic micromanipulation (CM) on the morphology and development of vitrified-warmed embryos as well as assisted reproductive technology (ART) outcomes. A total of 96 frozen embryo transfer (FET) cycles were included in this prospective randomized study. They were divided into three groups of CM (n=32), sham (n=32) and control (n=32). In the CM group, the vitrified- warmed embryos were subjected to fragments and coarse granules removal (cosmetic micromanipulation) after laser assisted zona hatching (LAH); sham group subjected only to LAH and no intervention was taken for the control group...
May 17, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28522355/closed-versus-open-vitrification-for-human-blastocyst-cryopreservation-a-meta-analysis
#10
Hyun Sik Youm, Jong-Ryeol Choi, Daesik Oh, Yong Ho Rho
Closed vitrification can minimize the risk of microbiological transmission through liquid nitrogen during the cooling, storage, and warming procedures. As cooling rates may reduce when closed vitrification is applied, clinical outcomes should be compared between closed and open vitrification in order to justify the use of closed vitrification. This study was conducted to investigate the differences in survival, implantation, clinical pregnancy, and live birth rates between closed and open vitrification for human blastocyst cryopreservation...
May 15, 2017: Cryobiology
https://www.readbyqxmd.com/read/28512855/insulin-like-growth-factor-i-enhances-the-developmental-competence-of-yak-embryos-by-modulating-aquaporin-3
#11
P Chen, Y Pan, Y Cui, Z Wen, P Liu, H He, Q Li, X Peng, T Zhao, S Yu
The objective of our present study was to determine the effects of insulin-like growth factor I (IGF-I) on the development of yak (Bos grunniens) embryos after cumulus-oocyte complex (COC) vitrification and warming followed by in vitro fertilization (IVF). In Experiment 1, the yak COCs underwent vitrification and then IVF. Embryos were incubated in synthetic oviductal fluid (SOF) supplemented with four concentrations (0, 50, 100 and 200 ng/ml) of IGF-I, while the yak COCs without vitrification or IGF-I supplementation acted as the control group; the BAX, BCL-2, AQP3mRNA and aquaporin 3 (AQP3) protein expression levels in the five groups of blastocysts were evaluated using quantitative real-time PCR and immunofluorescence analyses...
May 16, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28506797/-systematic-proposal-of-fertility-preservation-by-mature-oocyte-cryopreservation-for-recurrent-benign-ovarian-tumors
#12
Y Dadoun, H Azaïs, L Keller, E d'Orazio, P Collinet, C Decanter
OBJECTIVES: To investigate prospectively the pattern of the follicular growth and to characterize the COH outcome in terms of oocyte number and maturity in patients with voluminous recurrent benign ovarian tumors with a high surgical risk of significant reduction of the ovarian follicular content. METHODS: The inclusion criteria were: age between 18 and 36, presence of at least one benign ovarian tumor (≥ 5cm) with high risk of recurrence. The fertility preservation cycle was performed at least 3 months after the cyst surgery...
May 12, 2017: Gynecologie, Obstetrique, Fertilite & Senologie
https://www.readbyqxmd.com/read/28502527/effects-of-different-sucrose-concentrations-on-vitrified-porcine-preantral-follicles-qualitative-and-quantitative-analysis
#13
Paula Rocío Gabriel, Pablo Torres, María Celeste Fratto, Humberto Cisale, Juan Alberto Claver, Daniel Marcelo Lombardo, María Laura Fischman
The aim of the present study was to perform a qualitative and quantitative analysis of the effect of different sucrose concentrations combined with ethylene glycol in the preservation of vitrified porcine preantral follicles. Fragments of ovarian cortex were vitrified in cryotubes containing 200 μl of the vitrification solution (30% Ethylene Glycol; 20% Fetal Bovine Serum; 0 M-0.25 M - 0.75 M or 1 M sucrose) and stored in liquid nitrogen for a week. Histological analysis showed that after vitrification the number of normal follicles decreased compared to the fresh tissue (control)...
May 11, 2017: Cryobiology
https://www.readbyqxmd.com/read/28502309/effects-of-vitrification-of-cumulus-enclosed-porcine-oocytes-at-the-germinal-vesicle-stage-on-cumulus-expansion-nuclear-progression-and-cytoplasmic-maturation
#14
Ruth Appeltant, Tamás Somfai, Elisa C S Santos, Thanh Quang Dang-Nguyen, Takashi Nagai, Kazuhiro Kikuchi
Although offspring have been produced from porcine oocytes vitrified at the germinal vesicle (GV) stage, the rate of embryo development remains low. In the present study, nuclear morphology and progression, cumulus expansion, transzonal projections (TZPs), ATP and glutathione (GSH) levels were compared between vitrified cumulus-oocyte complexes (COCs) and control COCs (no cryoprotectant treatment and no cooling), as well as a toxicity control (no cooling). Vitrification was performed with 17.5% (v/v) ethylene glycol and 17...
May 15, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28500451/pregnancy-rates-for-single-embryo-transfer-set-of-day-5-and-day-6-blastocysts-after-cryopreservation-by-vitrification-and-slow-freeze
#15
Leah Kaye, Erica Anspach Will, Alison Bartolucci, John Nulsen, Claudio Benadiva, Lawrence Engmann
PURPOSE: The purpose of this study was to compare clinical and ongoing pregnancy rates in cycles with single embryo transfer (SET) of blastocysts cryopreserved on day 5 or day 6. Our aim was to determine whether day 6 blastocysts perform adequately to recommend SET. METHODS: Retrospective cohort study including 468 transfer cycles for 392 women younger than age 38 undergoing SET at a university-affiliated IVF clinic in the USA. A total of 261 day 5 blastocysts and 207 day 6 blastocysts for frozen-thawed SET between 2010 and 2016 were analyzed...
May 12, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28492328/inhibition-of-radiolytic-molecular-hydrogen-formation-by-quenching-of-excited-state-water
#16
Gregory P Horne, Simon M Pimblott, Jay A LaVerne
Comparison of experimental measurements of the yield of molecular hydrogen produced in the gamma radiolysis of water and aqueous nitrate solutions with predictions of a Monte Carlo track chemistry model shows that the nitrate anion scavenging of the hydrated electron, its precursor, and hydrogen atom cannot account for the observed decrease in the yield at high nitrate anion concentrations. Inclusion of the quenching of excited states of water (formed by either direct excitation or reaction of the water radical cation with the precursor to the hydrated electron) by the nitrate anion into the reaction scheme provides excellent agreement between the stochastic calculations and experiment demonstrating the existence of this short-lived species and its importance in water radiolysis...
May 23, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28487134/effect-of-vitrification-on-the-zona-pellucida-hardening-and-follistatin-and-cathepsin-b-genes-expression-and-developmental-competence-of-in-vitro-matured-bovine-oocytes
#17
Teresa Wiesak, Marta Wasielak, Aleksandra Złotkowska, Robert Milewski
The purpose of our study was to assess the effect of vitrification with or without the presence of calcium in the vitrification solution on the: 1) diameter of oocytes and thickness of the zona pellucida, 2) zona pellucida hardening, 3) expression of mRNA follistatin (FST) and cathepsin B (CTSB) in oocytes and 4) developmental competence of embryos derived from in vitro matured and vitrified oocytes. The results of our study demonstrate, that vitrification did not alter thickness of the zona pellucida and diameter of the oocytes, however it triggered hardening of the zona pellucida...
May 6, 2017: Cryobiology
https://www.readbyqxmd.com/read/28481448/bio-inspired-solute-enables-preservation-of-human-oocytes-using-minimum-volume-vitrification
#18
Jung Kyu Choi, Rami El Assal, Nicholas Ng, Elizabeth Ginsburg, Raymond M Anchan, Utkan Demirci
The ability to cryopreserve human oocytes has significant potential for fertility preservation. Current cryopreservation methods still suffer from the use of conventional cryoprotectants, such as dimethyl sulfoxide (DMSO), causing loss of viability and function. Such injuries result from the toxicity and high concentration of cryoprotectants as well as mechanical damage of cells due to ice crystal formation during the cooling and rewarming processes. Here, we report preservation of human oocytes following vitrification using an innovative bio-inspired cryoprotectant integrated with a minimum volume vitrification approach...
May 8, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28480936/high-density-equilibrium-phases-of-colloidal-ellipsoids-by-application-of-optically-enhanced-direct-current-electric-fields
#19
Mahesh Ganesan, Michael J Solomon
We use direct current (DC) electric fields in conjunction with ultraviolet light to self-assemble highly dense structures of colloidal ellipsoids with three-dimensional order and volume fraction as large as 67%. Ellipsoidal phases of colloids are of fundamental interest because novel packing structures are predicted to occur at high volume fractions; the symmetries of these crystal unit cells can also contribute to a variety of applications, including structural color materials. Previously, the very high volume fraction range of ellipsoidal phases has been inaccessible because of limitations such as vitrification and kinetic trapping...
May 24, 2017: Soft Matter
https://www.readbyqxmd.com/read/28479126/low-serum-concentration-in-bovine-embryo-culture-enhances-early-blastocyst-rates-on-day-6-with-quality-traits-in-the-expanded-blastocyst-stage-similar-to-bsa-cultured-embryos
#20
A Murillo, M Muñoz, D Martín-González, S Carrocera, A Martínez-Nistal, E Gómez
In bovine, single in vitro embryo culture in protein-free medium from Day-6 to Day-7 leads to expanded blastocyst (XB) with improved pregnancy and birth rates after cryopreservation. Under these conditions, early blastocysts (EB) progress to the XB stage at higher rates than morulae (M). However, embryo production with BSA in culture prior to Day-6 leads to low EB rates. We investigated whether a very low FCS concentration (0.1%) in culture from Day-1 to Day-6 would improve EB rates and, subsequently, increase XB rates on Day-7 after single culture in protein-free medium...
May 4, 2017: Reproductive Biology
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