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Vitrification

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https://www.readbyqxmd.com/read/28433371/in%C3%A2-vitro-fertilization-with-preimplantation-genetic-diagnosis-for-aneuploidies-in-advanced-maternal-age-a-randomized-controlled-study
#1
Carmen Rubio, José Bellver, Lorena Rodrigo, Gema Castillón, Alfredo Guillén, Carmina Vidal, Juan Giles, Marcos Ferrando, Sergio Cabanillas, José Remohí, Antonio Pellicer, Carlos Simón
OBJECTIVE: To determine the clinical value of preimplantation genetic diagnosis for aneuploidy screening (PGD-A) in women of advanced maternal age (AMA; between 38 and 41 years). DESIGN: This was a multicenter, randomized trial with two arms: a PGD-A group with blastocyst transfer, and a control group with blastocyst transfer without PGD-A. SETTING: Private reproductive centers. PATIENT(S): A total of 326 recruited patients fit the inclusion criteria, and 205 completed the study (100 in the PGD-A group and 105 in the control group)...
April 19, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28430440/insights-on-the-physical-state-reached-by-an-active-pharmaceutical-ingredient-upon-high-energy-milling
#2
Benjamin Schammé, Xavier Monnier, Nicolas Couvrat, Laurent Delbreilh, Valérie Dupray, Eric Dargent, Gérard Coquerel
In this article, we study the physicochemical transformations of crystalline Quinidine upon high-energy milling. The investigations have been achieved by classical, high performance and fast scanning calorimetry combined with broadband dielectric spectroscopy and X-ray powder diffraction. As evolution of crystalline Quinidine with time of milling revealed a prominent sub-Tg cold-crystallization phenomenon, independent and complementary analytical techniques were implemented. Fast scanning calorimetry was performed for the first time on a milled pharmaceutical compound to postpone the crystallization event to higher temperatures...
April 21, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28428938/effects-of-supplementation-with-antifreeze-proteins-on-the-follicular-integrity-of-vitrified-warmed-mouse-ovaries-comparison-of-two-types-of-antifreeze-proteins-alone-and-in-combination
#3
Min Kyung Kim, Hyun Sun Kong, Hye Won Youm, Byung Chul Jee
OBJECTIVE: The aim of this study was to analyze the effect of supplementing vitrification and warming solutions with two types of antifreeze proteins (AFPs) and the combination thereof on the follicular integrity of vitrified-warmed mouse ovaries. METHODS: Ovaries (n=154) were obtained from 5-week-old BDF1 female mice (n=77) and vitrified using ethylene glycol and dimethyl sulfoxide with the supplementation of 10 mg/mL of Flavobacterium frigoris ice-binding protein (FfIBP), 10 mg/mL of type III AFP, or the combination thereof...
March 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28428046/cryoprotectants-a-review-of-the-actions-and-applications-of-cryoprotective-solutes-that-modulate-cell-recovery-from-ultra-low-temperatures
#4
REVIEW
Gloria D Elliott, Shangping Wang, Barry J Fuller
Cryopreservation has become a central technology in many areas of clinical medicine, biotechnology, and species conservation within both plant and animal biology. Cryoprotective agents (CPAs) invariably play key roles in allowing cells to be processed for storage at deep cryogenic Temperatures and to be recovered with high levels of appropriate functionality. As such, these CPA solutes possess a wide range of metabolic and biophysical effects that are both necessary for their modes of action, and potentially complicating for cell biological function...
April 18, 2017: Cryobiology
https://www.readbyqxmd.com/read/28422434/rat-hindlimb-cryopreservation-and-transplantation-a-step-towards-organ-banking
#5
Amir Arav, Or Friedman, Yehudit Natan, Eyal Gur, Nir Shani
In 2016 over 5 million reconstructive procedures were performed in the USA. The recent successes of clinical vascularized composite allotransplantations (VCA), hand and face transplantations included, established the tremendous potential of these life-enhancing reconstructions. Nevertheless, Due to limited availability and lifelong immunosuppression, application is limited. Long term banking of composite transplants may increase the availability of esthetically compatible parts with partial or complete HLA matching, reducing the risk of rejection and the immunosuppressive burden...
April 19, 2017: American Journal of Transplantation
https://www.readbyqxmd.com/read/28421511/appendix-g-vitrification-of-blastocysts-using-vitriblast-tm-and-thermoblast-tm-nidacon
#6
Anna Niläng Laessker, Thorir Hardarsson, Ann-Sofie Forsberg, Tetsunori Mukaida, Paul V Holmes
This appendix describes the vitrification of blastocysts using VitriBlast™ (VBK) and ThermoBlast™ (TBK) from Nidacon, Mölndal, Sweden. The technique used and the reason for not including DMSO in the medium at the production stage, but including it separately in the kit, and the importance of collapsing the blastocyst prior to vitrification will be explained and described.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28421510/appendix-f-quinn-s-advantage-embryo-freeze-kit
#7
Kiri Beilby, Patrick Quinn
Despite a large focus on the use of vitrification to cryopreserve embryos in recent years, there are still arguments for the use of slow freezing for the cleavage-stage embryo. Having said this, there are lessons to be learned from the process of vitrification that could be applied to slow freezing to improve post-thaw survival and ultimately clinical pregnancy rates. Specifically, increasing the concentration of sucrose in the freezing solution from 0.1 to 0.2 M and subsequently increasing the sucrose concentrations in thawing solutions could prove beneficial...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28421509/appendix-e-rapid-i-tm-closed-vitrification-device-by-vitrolife
#8
Mark G Larman
Cryopreservation of gametes and embryos is a growing technique in numerous reproductive fields including human-assisted reproduction. With improved understanding of embryo physiology and optimized culture conditions, there are now more embryos than ever to vitrify for potential use in subsequent cycles. Many gametes and embryos have been cryopreserved in open systems, but there are concerns with regard to contamination from the liquid nitrogen and also cross-contamination between patients' germplasm. The development of the Rapid-i™, a closed vitrification device that does not use direct contact with liquid nitrogen during vitrification or subsequent storage, will be discussed as well as clinical protocols for human oocytes and embryos...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28421508/appendix-d-irvine-scientific-%C3%A2-vitrification-system
#9
Matthew VerMilyea, Amber Brewer
This chapter will describe the use of the Irvine Scientific(®) vitrification and warming solutions (Vit Kits(®)) along with a detailed protocol for the correct use of the CryoTip(®) vitrification device for human embryos and oocytes. Successful pregnancies have been reported after carrying out rapid vitrification methods of oocytes, cleavage stage embryos, and blastocysts using the CryoTip(®) (Popwell et al. Fertil Steril, 101:e20, 2014; Kuwayama et al. Reprod Biomed 11:608-614, 2005; Kuwayama et al. Fertil Steril 84:S187, 2005; Kuwayama et al...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28421507/appendix-c-automated-vitrification-of-mammalian-embryos-on-a-digital-microfluidic-device
#10
Jun Liu, Derek G Pyne, Mohamed Abdelgawad, Yu Sun
This chapter introduces a digital microfluidic device that automates sample preparation for mammalian embryo vitrification. Individual microdroplets manipulated on the microfluidic device were used as microvessels to transport a single mouse embryo through a complete vitrification procedure. Advantages of this approach, compared to manual operation and channel-based microfluidic vitrification, include automated operation, cryoprotectant concentration gradient generation, and feasibility of loading and retrieval of embryos...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28421506/appendix-b-solid-surface-vitrification
#11
Mohan S Kamath, K Muthukumar
Solid surface vitrification method involves direct contact of carrier loaded with droplet containing gametes or embryos with precooled metal surface. Over the years, following certain modifications, solid surface vitrification has emerged as an efficient method for vitrifying human gametes and embryos. Here, we describe the principle and methodology of solid surface vitrification.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28421505/appendix-a-cryotech-%C3%A2-vitrification-thawing
#12
Goral Gandhi, Masashige Kuwayama, Sakina Kagalwala, Priyanka Pangerkar
In the last 15-20 years, many centers are using vitrification as a method of choice for cryopreservation of human oocytes and embryos. As vitrification technologies have improved their success profiles, new applications seem to have emerged, making IVF treatments more successful and more flexible.This appendix describes the Cryotech(®) method, which is the latest "minimal volume approach" method, suitable for cryopreservation of oocytes and embryos of any developmental stage, including blastocysts. Dr. Masashige Kuwayama, who has introduced major advances in oocyte and embryo cryopreservation, has developed this method...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28421504/chapter-20-gavi-automated-vitrification-instrument
#13
Tammie K Roy, Susanna Brandi, Teija T Peura
Gavi is intended for use in a laboratory or clinic environment for the preparation and vitrification of oocytes, cleavage stage embryos and blastocysts. Gavi is designed to automate the equilibration steps in the vitrification process to minimize the variability that occurs during cryopreservation. This automated process reduces the potential for errors and ensures a standardized, repeatable procedure for vitrification in a controlled, closed-system environment.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28421502/chapter-18-vitrification-a-reliable-method-for-cryopreservation-of-animal-embryos
#14
B Singh, G Mal, S K Singla
The impact of cryopreservation in assisted reproduction is increasingly appreciated. Cells, oocytes, and embryos preserved under ultralow temperature can endure storage for indefinite time with almost no alteration in their metabolic and genetic components. Advances in cryopreservation and its applications to preserve cells, gametes, and embryos offer opportunities in conservation of biodiversity and dissemination of elite livestock germplasm. This chapter describes the vitrification for cryopreservation of livestock embryos...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28421501/chapter-17-sterile-plate-based-vitrification-of-adherent-human-pluripotent-stem-cells-and-their-derivatives-using-the-twist-method
#15
Julia C Neubauer, Frank Stracke, Heiko Zimmermann
Due to their high biological complexity, e.g., their close cell-to-cell contacts, cryopreservation of human pluripotent stem cells with standard slow-rate protocols often is inefficient and can hardly be standardized. Vitrification that means ultrafast freezing already showed very good viability and recovery rates for this sensitive cell system, but is only applicable for low cell numbers, bears a high risk of contamination, and can hardly be implemented under GxP regulations. In this chapter, a sterile plate-based vitrification method for adherent pluripotent stem cells and their derivatives is presented based on a procedure and device for human embryonic stem cells developed by Beier et al...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28421500/chapter-16-directional-freezing-of-ovarian-tissue-and-freeze-drying-of-stem-cells-for-fertility-preservation
#16
Amir Arav, Yehudit Natan
Fertility preservation is practiced today for women in their fertile years who wish or need to postpone or prolong their reproductive era. Currently women have two main options to preserve their fertility either by oocyte vitrification or by freezing of ovarian cortical slices. In this chapter we will describe the use of directional freezing for cortical slices and freeze-drying of stem cells. These procedures open the way for the preservation of reproductive cells and tissue for future use in fertility preservation treatments...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28421498/chapter-14-establishing-an-oocyte-cryobank-network
#17
James Graham, Josh Lim, Michael Tucker
Improvements in oocyte cryopreservation has prompted wider acceptance of this technology leading to its use for several reasons. Notably, in addition to elective and medically driven reasons for oocyte cryostorage for fertility preservation, donor oocyte cryobanking is beginning to gain traction, potentially replacing fresh oocyte donation in assisted reproduction. Donor "egg banking," while not totally analogous to donor sperm banking, does provide strong benefits in terms of scheduling flexibility and improved clinical efficiencies, while providing a wider immediate inventory choice...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28421497/chapter-13-human-ovarian-tissue-vitrification
#18
Sherman Silber
Ovarian freezing and transplantation has garnered increasing interest as a potential way of preserving fertility in cancer patients as well as for women who just wish to delay childbearing. This chapter spells out our techniques of ovarian cortex vitrification and results for frozen compared to fresh ovarian cortex transplantation (in one single series from one center for the sake of consistency), as well as potentially provides insight into the mechanism behind ovarian follicle recruitment. This represents an effort to simplify and popularize an approach that has yielded favorable results (all cases recovered ovulation and 75% had successful spontaneous pregnancy) in one single, disciplined study...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28421495/chapter-11-human-embryo-vitrification
#19
Juergen Liebermann
Cryopreservation is one of the keystones in clinical infertility treatment. In particular vitrification has become a well-established and widely used routine procedure that has allowed important expansion of therapeutic strategies when IVF is used to treat infertility. Vitrification of human blastocysts allows us to maximize the potential for conception from any single in vitro fertilization cycle and prevents wastage of embryos. The technology may even be used to eliminate fresh embryo transfers for reasons of convenience, uterine receptivity, fertility preservation, preimplantation genetic diagnosis, or emergency management...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28421494/chapter-10-human-oocyte-vitrification
#20
Laura Rienzi, Ana Cobo, Filippo Maria Ubaldi
Discovery and widespread application of successful cryopreservation methods for MII-phase oocytes was one of the greatest successes in human reproduction during the past decade. Although considerable improvements in traditional slow-rate freezing were also achieved, the real breakthrough was the result of introduction of vitrification. Here we describe the method that is most commonly applied for this purpose, provides consistent survival and in vitro developmental rates, results in pregnancy and birth rates comparable to those achievable with fresh oocytes, and does not result in higher incidence of gynecological or postnatal complications...
2017: Methods in Molecular Biology
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