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Vitrification

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https://www.readbyqxmd.com/read/28333312/establishment-maintenance-and-functional-integrity-of-the-blood-testis-barrier-in-organotypic-cultures-of-fresh-and-frozen-thawed-prepubertal-mouse-testes
#1
C Rondanino, A Maouche, L Dumont, A Oblette, N Rives
STUDY QUESTION: Can the spatio-temporal formation of an intact blood-testis barrier (BTB), which is essential for the progression of spermatogenesis, be reproduced in cultures of fresh or frozen/thawed prepubertal mouse testes? SUMMARY ANSWER: Organotypic cultures allow the establishment and maintenance of major BTB components and the formation of a functional BTB in mouse testicular tissues. WHAT IS KNOWN ALREADY: In vitro maturation of prepubertal testicular tissues is a promising approach to restore fertility in adult survivors of childhood cancer...
March 17, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28315146/attempts-to-improve-human-ovarian-transplantation-outcomes-of-needle-immersed-vitrification-and-slow-freezing-by-host-and-graft-treatments
#2
Ronit Abir, Benjamin Fisch, Noa Fisher, Nivin Samara, Galit Lerer-Serfaty, Roei Magen, Michal Herman-Edelstein, Avi Ben-Haroush, Anat Stein, Raoul Orvieto
PURPOSE: To investigate if needle-immersed vitrification or slow-freezing yields better implantation results for human ovarian tissue and which method benefits more when combined with the "improvement protocol" of host melatonin treatment and graft incubation with biological glue + vitamin E + vascular endothelial growth factor-A. METHODS: Human ovarian tissue was preserved by needle-immersed vitrification or slow-freezing and transplanted into immunodeficient mice, either untreated (groups A and C, respectively) or treated with the improvement protocol (groups B and D, respectively)...
March 18, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28302936/cryotolerance-of-porcine-blastocysts-is-improved-by-treating-in-vitro-matured-oocytes-with-l-carnitine-prior-to-fertilization
#3
Jenna L Lowe, Louise K Bartolac, Roslyn Bathgate, Christopher G Grupen
Sufficient generation of adenosine triphosphate (ATP) by oocytes is critical for fertilization and embryo development. The objective of this study was to determine the effects of supplementing media with L-carnitine, a co-factor required for the metabolism of fatty acids, during the peri-fertilization period on embryo development and energy generation. Firstly, in vitro fertilization (IVM) porcine oocytes were co-incubated with sperm in IVF medium supplemented with 0‒24 mM L-carnitine. The blastocyst formation rate of the control group was greater than those of the L-carnitine groups (P < 0...
March 16, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28295704/comparison-of-the-effects-of-ham-sf10-and-%C3%AE-mem-in-combination-with-fbs-or-bsa-in-vitrification-warming-solution-on-quality-and-viability-of-sheep-ovarian-follicles
#4
F Mohammadzadeh, L Safdarian, F Amidi, A Mohammadzadeh, K Mortezaee, S Mehdinejhadiani, A Sobhani, S Ghasemi, F Sargolzaei Aval
The aim of this study was to evaluate the effects of the two types of media, namely minimum essential medium (αMEM) and Ham'sF10, supplemented with foetal bovine serum (FBS) or bovine serum albumin (BSA) in vitrification/warming solution on the quality and viability of sheep ovarian follicles. Vitrification method was applied for cryopreservation of sheep ovarian cortex using Ham'sF10 and αMEM supplemented with either BSA or FBS. There were five groups: Fresh, Ham'sF10+ BSA, Ham'sF10+ FBS, αMEM + BSA and αMEM + FBS...
March 10, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28295644/effect-of-vitrification-on-the-mrna-transcriptome-of-bovine-oocytes
#5
N Wang, C-Y Li, H-B Zhu, H-S Hao, H-Y Wang, C-L Yan, S-J Zhao, W-H Du, D Wang, Y Liu, Y-W Pang, X-M Zhao
Vitrification has been shown to decrease the developmental capacity of mammalian oocytes, and this is closely associated with the abnormal mRNA expressions of vitrified oocytes. However, the effect of vitrification on transcriptional machinery of oocytes examined by RNA sequencing (RNA-seq) has yet to be defined. In the present study, the mRNA transcriptomes of fresh and vitrified bovine oocytes were analysed by Smart-seq2 with the differently expressed genes determined by DEseq2 (an adjusted p-value of .05 and a minimum fold change of 2)...
March 12, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28292612/perinatal-outcomes-in-children-born-after-fresh-or-frozen-embryo-transfer-a-catalan-cohort-study-based-on-14-262-newborns
#6
Mar Vidal, Kilian Vellvé, Mireia González-Comadran, Ana Robles, Maria Prat, Mar Torné, Ramón Carreras, Miguel A Checa
OBJECTIVE: To ascertain whether perinatal outcomes are affected by vitrification and/or controlled ovarian hyperstimulation (COH). DESIGN: Register-based cohort study. SETTING: Not applicable. PATIENT(S): Women undergoing in vitro fertilization (IVF) between 2008 and 2012 using autologous or donated eggs who had a singleton pregnancy delivered from the 24th week onward. INTERVENTION(S): Fresh embryo transfer (ET) or frozen-thawed ET in women undergoing IVF...
March 11, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28287560/modified-microsecure-vitrification-a-safe-simple-and-highly-effective-cryopreservation-procedure-for-human-blastocysts
#7
Mitchel C Schiewe, Shane Zozula, Nancy Nugent, Kelley Waggoner, Jessica Borba, Lisa Gamboa, John B Whitney
Clinical embryo vitrification evolved with the development of unique vitrification devices in the 21(st) century and with the misconception that ultra-rapid cooling in an "open" system (i.e., direct LN2 contact) was a necessity to optimize vitrification success. The dogma surrounding the importance of cooling rates led to unsafe practices subject to technical variation and to the creation of vitrification devices that disregarded important quality-control factors (e.g., ease of use, repeatability, reliability, labeling security, and storage safety)...
March 2, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28283337/vitrification-of-porcine-immature-oocytes-association-of-equilibration-manners-with-warming-procedures-and-permeating-cryoprotectants-effects-under-two-temperatures
#8
Guoquan Wu, Baoyu Jia, Guobo Quan, Decai Xiang, Bin Zhang, Qingyong Shao, Qionghua Hong
The aim of this study was to evaluate the association of equilibration manners with warming procedures, and the different permeating cryoprotectants (pCPAs) effects under two temperatures, in terms of survival, maturation and subsequent parthenogenetic development of porcine immature oocytes after Cryotop vitrification. In Experiment 1, oocytes were equilibrated by exposure to 5% (v/v) ethylene glycol (EG) for 10 min (EM1) or stepwise to 7.5% (v/v) and 15% (v/v) EG for 2.5 min respectively (EM2). Warming procedures were performed in 1...
March 7, 2017: Cryobiology
https://www.readbyqxmd.com/read/28277744/a-modified-vitrification-method-reduces-spindle-and-chromosome-abnormalities
#9
Minghui Liu, Wenhui Zhou, Dapeng Chu, Lei Fu, Wei Sha, Shan Liu, Yuan Li
Development of an effective system for oocyte-cryopreservation is of clinical relevance in reproductive medicine. However, oocyte-preservation is not as effective as embryo preservation. In this study, we used a 37°C pre-equilibrium temperature as part of a modified vitrification method for human oocyte cryopreservation. The effect of the new method on spindle configuration, chromosomal arrangement, and mitochondrial distribution was investigated in in vitro-matured human oocytes. A total of 101 in vitro-matured oocytes were randomly assigned for vitrification at pre-equilibrium temperature of 37°C (37°C Group, n=50) or at room temperature (RT Group, 22-24°C, n=51)...
March 9, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28266941/subzero-organ-preservation-the-dawn-of-a-new-ice-age
#10
Bote G Bruinsma, Korkut Uygun
PURPOSE OF REVIEW: Herein, we review the field of subzero organ preservation with a focus on recent developments in hepatic supercooling. RECENT FINDINGS: Organ preservation is making a rapid shift from the decade old standard of storage on ice toward techniques that improve organ availability as well as preservation time. Long-term organ preservation would have tremendous benefits to the organ transplantation field, including better organ allocation, donor-recipient matching, as well as reduced preservation injury, and subsequent improvement of donor organ use...
March 6, 2017: Current Opinion in Organ Transplantation
https://www.readbyqxmd.com/read/28260212/conservation-of-somatic-tissue-derived-from-collared-peccaries-pecari-tajacu-linnaeus-1758-using-direct-or-solid-surface-vitrification-techniques
#11
Alana Azevedo Borges, Gabriela Liberalino Lima, Luiza Bento de Queiroz Neta, Maria Valéria de Oliveira Santos, Moacir Franco de Oliveira, Alexandre Rodrigues Silva, Alexsandra Fernandes Pereira
Cryopreservation of somatic tissue can be applied in biodiversity conservation, especially for wild species as collared peccary. We aimed to evaluate the effect of vitrification techniques of ear tissue of collared peccary [direct vitrification in cryovials (DVC) or solid-surface vitrification (SSV)] on the layers of epidermis and dermis by conventional histology and cell ability during the in vitro culture. Thus, both the vitrification methods were able to maintain normal patterns of the epidermis as the cornea and granular layers, furthermore the intercellular space and dermal-epidermal junction of the spinous layer when compared to fresh control...
March 4, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28256523/production-of-the-medaka-derived-from-vitrified-whole-testes-by-germ-cell-transplantation
#12
Shinsuke Seki, Kazunari Kusano, Seungki Lee, Yoshiko Iwasaki, Masaru Yagisawa, Mariko Ishida, Tadashi Hiratsuka, Takao Sasado, Kiyoshi Naruse, Goro Yoshizaki
The medaka (Oryzias latipes) is a teleost model distinguished from other model organisms by the presence of inbred strains, wild stocks, and related species. Cryopreservation guarantees preservation of these unique biological resources. However, because of their large size, cryopreservation techniques for their eggs and embryos have not been established. In the present study, we established a methodology to produce functional gametes from cryopreserved testicular cells (TCs). Whole testes taken from medaka were cryopreserved by vitrification...
March 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28254381/vitrification-after-multiple-rounds-of-sample-application-and-blotting-improves-particle-density-on-cryo-electron-microscopy-grids
#13
Joost Snijder, Andrew J Borst, Annie Dosey, Alexandra C Walls, Anika Burrell, Vijay S Reddy, Justin M Kollman, David Veesler
Single particle cryo-electron microscopy (cryoEM) is becoming widely adopted as a tool for structural characterization of biomolecules at near-atomic resolution. Vitrification of the sample to obtain a dense distribution of particles within a single field of view remains a major bottleneck for the success of such experiments. Here, we describe a simple and cost-effective method to increase the density of frozen-hydrated particles on grids with holey carbon support films. It relies on performing multiple rounds of sample application and blotting prior to plunge freezing in liquid ethane...
February 22, 2017: Journal of Structural Biology
https://www.readbyqxmd.com/read/28251904/improved-tissue-cryopreservation-using-inductive-heating-of-magnetic-nanoparticles
#14
Navid Manuchehrabadi, Zhe Gao, Jinjin Zhang, Hattie L Ring, Qi Shao, Feng Liu, Michael McDermott, Alex Fok, Yoed Rabin, Kelvin G M Brockbank, Michael Garwood, Christy L Haynes, John C Bischof
Vitrification, a kinetic process of liquid solidification into glass, poses many potential benefits for tissue cryopreservation including indefinite storage, banking, and facilitation of tissue matching for transplantation. To date, however, successful rewarming of tissues vitrified in VS55, a cryoprotectant solution, can only be achieved by convective warming of small volumes on the order of 1 ml. Successful rewarming requires both uniform and fast rates to reduce thermal mechanical stress and cracks, and to prevent rewarming phase crystallization...
March 1, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28241901/effects-of-antifreeze-glycoprotein-8-afgp8-supplementation-during-vitrification-on-the-in-vitro-developmental-capacity-of-expanded-bovine-blastocysts
#15
Shuang Liang, Bao Yuan, Yong-Xun Jin, Jia-Bao Zhang, Jeong Kyu Bang, Nam-Hyung Kim
Cryopreservation is an effective method for the long-term storage of valuable germplasm in the field of reproductive research. The present study examined the developmental capacity of post-thaw bovine blastocysts during vitrification after supplementation with antifreeze glycoprotein 8 (AFGP8). Survival and re-expansion rates in culture during the 12h after thawing were significantly higher in the AFGP8-treated than untreated group. In addition, blastocysts from the AFGP8-treated group exhibited lower rates of apoptosis...
February 28, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28240225/the-microscopic-origin-of-the-extreme-glass-forming-ability-of-albite-and-b2o3
#16
Edgar D Zanotto, Daniel R Cassar
Understanding the conditions that favour crystallisation and vitrification has been a longstanding scientific endeavour. Here we demonstrate that the extremely high glass-forming ability of unseeded supercooled Na2O·Al2O3·6SiO2 (Albite) and B2O3-known for decades as "crystallisation anomaly"-is caused by insufficient crystal nucleation. The predicted temperatures of the maximum homogeneous nucleation rates are located well below their glass transition temperatures (Tg), in a region of very high viscosity, which leads to extremely long nucleation time-lags and low nucleation rates...
February 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28237626/effects-of-cryopreservation-at-various-temperatures-on-the-survival-of-kelp-grouper-epinephelus-moara-embryos-from-fertilization-with-cryopreserved-sperm
#17
Yongsheng Tian, Zhangfan Chen, Jiang Tang, Huimin Duan, Jieming Zhai, Bo Li, Wenhui Ma, Jiangchun Liu, Yunxia Hou, Zhengxiang Sun
Fish embryo cryopreservation is highly important for the long-term preservation of genomic and genetic information; however, few successful cases of fish embryo cryopreservation have been reported over the past 60 years. This is the first study to use Epinephelus moara embryos from fertilization with cryopreserved sperm as experimental material. Embryos that developed to the 16-22 somite stage and tail-bud stage were treated with the vitrification solution PMG3T according to a five-step equilibration method and cryopreserved at various temperatures and storage duration...
February 22, 2017: Cryobiology
https://www.readbyqxmd.com/read/28228962/conforming-with-current-regulation-in-turkey-regarding-the-freezing-of-oocytes-a-case-report-of-the-first-pregnancy-in-turkey-achieved-through-oocyte-vitrification
#18
Semra Kahraman, Kahraman Ulker, Caroline Pirkevi Çetinkaya, Yesim Kumtepe Colakoglu, Hakan K Yelke, Cihangir Yilanlioglu
OBJECTIVES: To present the first pregnancy achieved in Turkey with frozen-warmed oocytes in a case with previous nine unsuccessful assisted reproductive technology (ART) attempts. METHODS: The clinical follow-up of a 33-year-old female applying to our ART centre after a long and complicated history of infertility is described. RESULTS: In April 2013, the woman attempted our centre for her 10th ART trial. She informed us on oocyte pick-up (OPU) day that her husband had been hospitalized following a car crush in Albania and was unable to travel to our clinic to give a sperm sample...
2017: SAGE open medical case reports
https://www.readbyqxmd.com/read/28215674/effect-of-vitrification-on-meiotic-maturation-mitochondrial-distribution-and-glutathione-synthesis-in-immature-silver-fox-cumulus-oocyte-complexes
#19
Xinyan Cao, Jingchun Li, Hailong Xue, Shiyong Wang, Weigang Zhao, Zhanyu Du, Yifeng Yang, Zhigang Yue
The present study was designed to investigate the effects of vitrifying oocytes obtained from silver foxes on nuclear maturation, mitochondrial distribution and glutathione (GSH) synthesis after in vitro culture for 72 h. Immature oocytes were randomly divided into three groups: (1) fresh GV (germinal vesicle) oocytes (Control group), (2) exposure to the equilibration and vitrification solution but without being plunged into liquid nitrogen (exposed group), and (3) vitrification by the cryoloop method (vitrified-warmed group)...
March 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28210478/the-twin-epidemic-in-infertility-care-why-do-we-persist-in-transferring-too-many-embryos
#20
EDITORIAL
W Ombelet
The epidemic of iatrogenic multiple births as a result of infertility treatment are responsible for an unacceptable high incidence of maternal, perinatal and childhood morbidity and mortality. Healthcare costs due to infertility therapy are too high and this may lead to social and political concern. The introduction of single embryo transfer (SET) was a real breakthrough, but was only accepted in most European countries and Japan. The United States, Latin America and most developing countries still have high multiple pregnancy rates...
December 2016: Facts, Views & Vision in ObGyn
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