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Vitrification

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https://www.readbyqxmd.com/read/28647785/fertility-preservation-for-trans-men-frozen-thawed-in-vitro-matured-oocytes-collected-at-the-time-of-ovarian-tissue-processing-exhibit-normal-meiotic%C3%A2-spindles
#1
S Lierman, K Tilleman, K Braeckmans, K Peynshaert, S Weyers, G T'Sjoen, P De Sutter
PURPOSES: At the moment of sex reassignment surgery (SRS), the ovarian tissue is sometimes cryopreserved as fertility preservation option for female-to-male trans men, also called trans men. During this preparation, cumulus-oocyte-complexes (COCs) can be found and in vitro matured. It is not known if these oocytes are developmentally competent. In order to use these oocytes for fertility preservation and subsequent fertilization, a normal spindle structure before and after vitrification is necessary...
June 24, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28645837/residual-ethylene-glycol-and-dimethyl-sulphoxide-concentration-in-human-ovarian-tissue-during-warming-thawing-steps-following-cryopreservation
#2
Yusuke Nakamura, Ryuichiro Obata, Noriyuki Okuyama, Nobuya Aono, Tomoko Hashimoto, Koichi Kyono
There have been 60 births after transplantation of cryopreserved ovarian tissue: 58 using the slow freezing method, and two using the vitrification method. DMSO and EG are widely used as cryoprotectants. However DMSO is a known epimutagen, and EG has been reported to be toxic in high concentrations. In this study, we measured residual DMSO and EG in ovarian tissue after vitrification and slow freezing. Cryoprotectants remained at a high concentration in the vitrified/warmed ovarian tissue just before transplantation (DMSO: 9...
June 6, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28631074/no-difference-in-mitochondrial-distribution-is-observed-in-human-oocytes-after-cryopreservation
#3
Martin Stimpfel, Eda Vrtacnik-Bokal, Irma Virant-Klun
PURPOSE: The primary aim of this study was to determine if any difference in mitochondrial distribution can be observed between fresh and cryopreserved (slow-frozen/thawed and vitrified/warmed) oocytes when oocytes are stained with Mitotracker Red CMXRos and observed under a conventional fluorescent microscope. Additionally, the influence of cryopreservation procedure on the viable rates of oocytes at different maturation stages was evaluated. METHODS: The germinal vesicle (GV) and MII oocytes were cryopreserved with slow-freezing and vitrification...
June 19, 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/28625759/predictive-value-of-serum-hcg-concentrations-in-pregnancies-achieved-after-single-fresh-or-vitrified-warmed-blastocyst-transfer
#4
Galia Oron, Tal Shavit, Efrat Esh-Broder, Son Weon-Young, Togas Tulandi, Hananel Holzer
Possible differences between serum HCG levels in pregnancies achieved after transfer of a single fresh or a vitrified-warmed blastocyst were evaluated. Out of 1130 single blastocyst transfers resulting in positive HCG results, 789 were single fresh blastocyst transfers and 341 single vitrified-warmed blastocyst transfers. The initial serum HCG levels of 869 clinical intrauterine pregnancies were evaluated, 638 after the transfer of a single fresh blastocysts and 231 after the transfer of a single vitrified-warmed blastocysts...
May 30, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28617148/outcomes-of-ivf-cycles-coupled-with-pgs-by-acgh-of-embryos-from-donor-and-autologous-oocytes-transferred-after-vitrification-to-women-of-advanced-maternal-age
#5
Elena M Fedorova, Svetlana A Shlykova, Ksenia V Shunkina, Olga G Zaitceva, Elena N Lapina, Taras V Yanchuk, Alla S Kalugina
It is well documented that aneuploidy rate in preimplantation embryos increases with the mother's age, and at the same time the number of oocytes diminishes. Consequently, for patients of advanced maternal age two options are available to overcome these limitations: use of oocytes from young donors, or use of own oocytes coupled with preimplantation genetic screening (PGS) for 24 chromosomes. However, it is not clear which strategy might be more effective. The aim of this retrospective study was to evaluate outcomes of IVF cycles coupled with transfer of vitrified embryos from donor or autologous oocytes, both with or without PGS...
June 15, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28601150/progress-and-challenges-of-fish-sperm-vitrification-a-mini-review
#6
REVIEW
Miaomiao Xin, Mohammad Abdul Momin Siddique, Borys Dzyuba, Rafael Cuevas-Uribe, Anna Shaliutina-Kolešová, Otomar Linhart
To survive low temperature is required for a long-term storage (cryopreservation), cells should be vitrified to a state in which intracellular water is solidified without ice crystal formation. Two different approaches are described for fish sperm cryopreservation: 1) sperm conventional cryopreservation, in which extracellular water is partially crystallized and 2) sperm vitrification, in which both intra- and extra-cellular liquids are vitrified. Sperm vitrification has been applied to some fish species with limited success...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28596081/vitamin-k2-improves-developmental-competency-and-cryo-tolerance-of-in-vitro-derived-ovine-blastocyst
#7
Fatemeh Sefid, S Ostadhosseini, S M Hosseini, F Ghazvini Zadegan, M Pezhman, Mohammad Hossein Nasr Esfahani
Vitamin K2 (VK2), acts as an electron carrier in mitochondria and thereby effects reactive oxygen species (ROS) and ATP production. This study evaluates role of VK2 on in vitro developmental competency and cryo-survival of pre-implantation ovine embryos. Initially the optimal and beneficial concentration of VK2 on compaction and blastocyst formation rates was defined (0.1 μM). Subsequently, it was shown that 0.1 μM VK2, at blastocyst stage, reduces H2O2 production, increase the expression of mitochondrial related gene and improved embryos quality...
June 5, 2017: Cryobiology
https://www.readbyqxmd.com/read/28583597/equine-ovarian-tissue-viability-after-cryopreservation-and-in%C3%A2-vitro-culture
#8
G D A Gastal, F L N Aguiar, B G Alves, K A Alves, S G S de Tarso, G M Ishak, C A Cavinder, J M Feugang, E L Gastal
Ovarian tissue cryopreservation allows the preservation of the female fertility potential for an undetermined period. The objectives of this study were to compare the efficiency of cryoprotective agents (CPAs; dimethyl sulfoxide, DMSO; ethylene glycol, EG; and propylene glycol, PROH) using slow-freezing and vitrification methods, and evaluate the viability of cryopreserved equine ovarian tissue after 7 days of culture. Fresh and cryopreserved ovarian fragments were evaluated for preantral follicle morphology, stromal cell density, EGFR, Ki-67, Bax, and Bcl-2 protein expression, and DNA fragmentation...
July 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28571337/irradiation-vs-vitrification-induced-disordering-the-case-of
#9
N M Anoop Krishnan, Bu Wang, Yann Le Pape, Gaurav Sant, Mathieu Bauchy
Irradiation and vitrification can both result in the disordering of minerals. However, it remains unclear whether these effects are comparable or if the glassy state represents an upper limit for irradiation-induced disordering. By reactive molecular dynamics simulations, we compare the structure of irradiated quartz to that of glassy silica. We show that although they share some degree of similarity, the structure of irradiated quartz and glassy silica differs from each other, both at the short- (<3 Å) and the medium-range (>3 Å and <10 Å)...
May 28, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28571335/how-thermal-stress-alters-the-confinement-of-polymers-vitrificated-in-nanopores
#10
Chao Teng, Linling Li, Yong Wang, Rong Wang, Wei Chen, Xiaoliang Wang, Gi Xue
Understanding and controlling the glass transition temperature (Tg) and dynamics of polymers in confined geometries are of significance in both academia and industry. Here, we investigate how the thermal stress induced by a mismatch in the coefficient of thermal expansion affects the Tg behavior of polystyrene (PS) nanorods located inside cylindrical alumina nanopores. The size effects and molecular weight dependence of the Tg are also studied. A multi-step relaxation process was employed to study the relationship between thermal stress and cooling rate...
May 28, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28571320/combining-precipitation-and-vitrification-to-control-the-number-of-surface-patches-on-polymer-nanocolloids
#11
Chris Sosa, Victoria E Lee, Lorena S Grundy, Mary J Burroughs, Rui Liu, Robert K Prud'homme, Rodney D Priestley
In an effort to incorporate increasingly higher levels of functionality into soft nanoparticles, heterogeneously structured particles stand out as a simple means to enhance functionality by tailoring only particle architecture. Various means exist for the fabrication of particles with specific structural configurations; however, the tunability of particle morphology is still a challenging and often laborious task, especially in self-assembled systems where a single equilibrium configuration dominates. Improved strategies for multipatch particle assembly are therefore needed to allow for the tailoring of particle structure via a single, continuous assembly route...
June 1, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28546898/treatment-of-fly-ash-from-power-plants-using-thermal-plasma
#12
Sulaiman Al-Mayman, Ibrahim AlShunaifi, Abdullah Albeladi, Imed Ghiloufi, Saud Binjuwair
Fly ash from power plants is very toxic because it contains heavy metals. In this study fly ash was treated with a thermal plasma. Before their treatment, the fly ash was analyzed by many technics such as X-ray fluorescence, CHN elemental analysis, inductively coupled plasma atomic emission spectroscopy and scanning electron microscopy. With these technics, the composition, the chemical and physical proprieties of fly ash are determined. The results obtained by these analysis show that fly ash is mainly composed of carbon, and it contains also sulfur and metals such as V, Ca, Mg, Na, Fe, Ni, and Rh...
2017: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/28545999/a-comparative-analysis-of-the-efficacy-of-three-cryopreservation-protocols-on-the-survival-of-in-vitro-derived-cattle-embryos-at-pronuclear-and-blastocyst-stages
#13
Van Huong Do, Simon Walton, Sally Catt, Andrew W Taylor-Robinson
The effectiveness of three cryopreservation protocols (slow freezing, short equilibration vitrification and long equilibration vitrification) on in vitro-derived cattle embryos at expanded blastocyst and pronuclear stages was compared. 199 expanded blastocysts of good quality were assigned randomly into four treatment groups [control, non-cryopreserved (fresh, unfrozen); and the three cryopreservation methods]. The re-expansion of the cryopreserved blastocysts after 24 h in vitro culture was similar to that of the fresh control group...
May 22, 2017: Cryobiology
https://www.readbyqxmd.com/read/28543267/trehalose-sustains-a-higher-post-thaw-sperm-motility-than-sucrose-in-vitrified-human-sperm
#14
M Schulz, J Risopatrón, G Matus, E Pineda, C Rojas, V Isachenko, E Isachenko, R Sánchez
One of the cryopreservation methods that best preserves sperm function is vitrification. However, comparative studies have not been performed to evaluate the effect of nonpermeable cryoprotectors on sperm function for prolonged periods of time post-devitrification. These times are necessary, especially in in vitro fertilisation and intrauterine insemination, for gamete interaction and then fertilisation to occur, while maintaining motility to arrive at the fertilisation site. In this study, sucrose (.25 m) and trehalose (...
May 25, 2017: Andrologia
https://www.readbyqxmd.com/read/28534059/cryo-mesh-a-simple-alternative-cryopreservation-protocol
#15
B Funnekotter, E Bunn, R L Mancera
BACKGROUND: The continued development of new cryopreservation protocols has improved post-cryogenic success rates for a wide variety of plant species. Methods like the cryo-plate have proven beneficial in simplifying the cryopreservation procedure. OBJECTIVE: This study assessed the practicality of a stainless steel mesh strip (cryo-mesh) for cryopreserving shoot tips from Anigozanthos viridis. MATERIALS AND METHODS: Shoot tips of A. viridis (Kangaroo Paw) were precultured on 0...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28534056/carbon-nanomaterials-enhance-survival-of-agapanthus-praecox-callus-after-cryopreservation-by-vitrification
#16
S M Chen, L Ren, D Zhang, Y F Zhang, X H Shen
BACKGROUND: Adding exogenous compounds is an effective way to improve cell survival after cryopreservation. Carbon nanomaterials (CNMs) are novel exogenous substances with small particle size and good biocompatibility. OBJECTIVE: In this work, four types of CNMs were used for the cryopreservation of Agapanthus praecox callus and their possible effects and mechanism of action were analyzed. MATERIALS AND METHODS: The thermal properties of the vitrification solutions tested were detected by differential scanning calorimetry...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28534054/the-presence-of-zinc-in-the-mouse-ovary-vitrification-medium-histological-evaluation-and-follicle-growth
#17
S Geravandi, M Azadbakht
BACKGROUND: Cryopreservation of ovary is a relevant option for preserving fertility of cancer patients undergoing chemotherapy. OBJECTIVE: To evaluate the effect of zinc in the vitrification medium on histology and follicle growth. MATERIALS AND METHODS: Mouse ovaries were vitrified in vitrification medium supplemented with 0, 100, 150 or 200 μg/dl zinc, identified as V0, V1, V2 and V3 groups, respectively. Histological evaluation of ovaries was carried out...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28534050/cryopreservation-of-lilium-martagon-l-meristems-by-droplet-vitrification-and-evaluation-of-their-physiological-stability
#18
M Urbaniec-Kiepura, A Bach
BACKGROUND: The aim of the present study was to compare different strategies for cryopreservation of martagon lily meristems and to evaluate the physiological status of the regenerants. MATERIALS AND METHODS: The bulblets were stored at 5 degree C or 20 degree C and pretreated with 3% or 6% sucrose prior to droplet-vitrification. The meristems were then assessed for their survival and regeneration. Their photochemical activity was investigated using a Photosynthesis Yield Analyzer MINI PAM 2000 Portable Chlorophyll Fluorometer and their photosynthesis oxygen production was evaluated with a Plant Vital 5030 device...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28531193/raman-microscopy-investigation-of-vitrification-induced-structural-damages-in-mature-bovine-oocytes
#19
Giulia Rusciano, Carolina De Canditiis, Gianluigi Zito, Marcello Rubessa, Maria Serena Roca, Rosa Carotenuto, Antonio Sasso, Bianca Gasparrini
Although oocyte cryopreservation has great potentials in the field of reproductive technologies, it still is an open challenge in the majority of domestic animals and little is known on the biochemical transformation induced by this process in the different cellular compartments. Raman micro-spectroscopy allows the non-invasive evaluation of the molecular composition of cells, based on the inelastic scattering of laser photons by vibrating molecules. The aim of this work was to assess the biochemical modifications of both the zona pellucida and cytoplasm of vitrified/warmed in vitro matured bovine oocytes at different post-warming times...
2017: PloS One
https://www.readbyqxmd.com/read/28527625/cosmetic-micromanipulation-of-vitrified-warmed-cleavage-stage-embryos-does-not-improve-art-outcomes-an-ultrastructural-study-of-fragments
#20
Somayyeh Safari, Mohammad Ali Khalili, Zeinab Barekati, Iman Halvaei, Morteza Anvari, Stefania A Nottola
The aim was to study the ultrastructure of cytoplasmic fragments along with the effect of cosmetic micromanipulation (CM) on the morphology and development of vitrified-warmed embryos as well as assisted reproductive technology (ART) outcomes. A total of 96 frozen embryo transfer (FET) cycles were included in this prospective randomized study. They were divided into three groups of CM (n=32), sham (n=32) and control (n=32). In the CM group, the vitrified- warmed embryos were subjected to fragments and coarse granules removal (cosmetic micromanipulation) after laser assisted zona hatching (LAH); sham group subjected only to LAH and no intervention was taken for the control group...
May 17, 2017: Reproductive Biology
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