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https://www.readbyqxmd.com/read/28735008/trehalose-induced-structural-modulation-of-bovine-serum-albumin-at-ambient-temperature
#1
Ahana Das, Pijush Basak, Rudradip Pattanayak, Turban Kar, Rajib Majumder, Debadrita Pal, Anindita Bhattacharya, Maitree Bhattacharyya, Samudra Prosad Banik
Trehalose is a well-known protein stabilizing osmolyte. The present study has been designed to understand the interaction of trehalose with BSA at ambient temperature. Steady state fluorescence and life-time analysis along with CD, DLS and ITC have been employed to show that trehalose causes surface-associated structural perturbation of BSA to promote its compaction. Trehalose at 0.1M concentration resulted in increased solvent exposure of one of the two tryptophans of BSA with a 5nm redshift in emission and enhanced susceptibility to acrylamide quenching with an increase in KSV from 2...
July 19, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28726246/cryo-electron-tomography-the-cell-biology-that-came-in-from-the-cold
#2
REVIEW
Jonathan Wagner, Miroslava Schaffer, Rubén Fernández-Busnadiego
Cryo-electron tomography (cryo-ET) provides high resolution 3D views into cells pristinely preserved by vitrification. Recent technical advances such as direct electron detectors, the Volta phase plate and cryo-focused ion beam milling have dramatically pushed image quality and expanded the range of cryo-ET applications. Cryo-ET not only allows mapping the positions and interactions of macromolecules within their intact cellular context, but can also reveal their in situ structure at increasing resolution. Here, we review how recent work using cutting-edge cryo-ET technologies is starting to provide fresh views into different aspects of cellular biology at an unprecedented level of detail...
July 20, 2017: FEBS Letters
https://www.readbyqxmd.com/read/28716599/maintaining-viability-and-characteristics-of-cholangiocarcinoma-tissue-by-vitrification-based-cryopreservation
#3
Min Zeng, Qiu-Rui Yang, Gong-Bo Fu, Yuan Zhang, Xu Zhou, Wei-Jian Huang, Hong-Dan Zhang, Wei-Jian Li, Zhen-Yu Wang, He-Xin Yan, Bo Zhai
Tumor tissue has great clinical and scientific value which relies highly on the proper preservation of primary materials. Conventional tumor tissue cryopreservation using slow-freezing method has yielded limited success, leading to significant cell loss and morphological damage. Here we report a standardized vitrification-based cryopreservation method, by which we have successfully vitrified and warmed 35 intrahepatic cholangiocarcinoma (ICC) tissues with up to 80% viability of the fresh tumor tissues. Cryopreserved ICC tissue could generate patient-derived xenografts (PDXs) with take rates of 68...
July 14, 2017: Cryobiology
https://www.readbyqxmd.com/read/28696244/effect-of-vitrification-on-in-vitro-development-and-imprinted-gene-grb10-in-mouse-embryos
#4
Jianfeng Yao, Lixia Geng, Rongfu Huang, Weilin Peng, Xuan Chen, Xiaohong Jiang, Miao Yu, Ming Li, Yanfang Huang, Xiaoyu Yang
Vitrification of embryos is a routine procedure in IVF (in vitro fertilization) laboratories. In the present study, we aimed to investigate the effect of vitrification on mouse preimplantation embryo development in vitro, and effect on the epigenetic status of imprinted gene Grb10 in mouse embryos. The blastocyst formation rate for vitrified 8-cell embryos was similar to the non-vitrified 8-cell embryos, whereas the blastocyst hatching rate was lower than that of the non-vitrified group. The expression level of Grb10 major-type transcript decreased significantly in vitrified blastocysts compared with non-vitrified and in vivo blastocysts...
September 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28693953/cryopreservation-of-luciola-praeusta-kiesenwetter-coleoptera-lampyridae-embryos-by-vitrification
#5
Kang Li, Xuanli Chen, Xiaoling Song, Xi Wu, Yi Xian
There is an urgent need to preserve the ever-decreasing number of different species of fireflies all over the world. We sought to develop a vitrification procedure to cryopreserve the firefly embryos. The late stages of Luciola praeusta Kiesenwetter embryos were collected. Several impermeable and permeable protectants with various concentrations in different mediums (TNM-FH insect medium, Grace's medium, Dulbecco's Modification of Eagle's Medium (DMEM) and Dulbecco's Phosphate-Buffered Saline (DPBS)) was used...
July 7, 2017: Cryobiology
https://www.readbyqxmd.com/read/28686720/free-cholesterol-and-cholesterol-esters-in-bovine-oocytes-implications-in-survival-and-membrane-raft-organization-after-cryopreservation
#6
Jorgelina Buschiazzo, Glenda L Ríos, Jesica R Canizo, Silvia S Antollini, Ricardo H Alberio
Part of the damage caused by cryopreservation of mammalian oocytes occurs at the plasma membrane. The addition of cholesterol to cell membranes as a strategy to make it more tolerant to cryopreservation has been little addressed in oocytes. In order to increase the survival of bovine oocytes after cryopreservation, we proposed not only to increase cholesterol level of oocyte membranes before vitrification but also to remove the added cholesterol after warming, thus recovering its original level. Results from our study showed that modulation of membrane cholesterol by methyl-β-cyclodextrin (MβCD) did not affect the apoptotic status of oocytes and improved viability after vitrification yielding levels of apoptosis closer to those of fresh oocytes...
2017: PloS One
https://www.readbyqxmd.com/read/28683583/successful-controlled-ovarian-stimulation-and-vitrification-of-oocytes-in-an-adolescent-diagnosed-with-myelodysplastic-pre-malignant-clone-with-monosomy-7
#7
Valerie Laura Peddie, Abha Maheshwari
An improvement in long-term outcomes for malignancies and non-malignant conditions, together with a review of the NICE Guideline, has led to a need to provide clinical services to deal with the sequelae of disease, its treatment, and subsequent survival of young people diagnosed with cancer. In this article, we describe fertility preservation in an adolescent female diagnosed with Myelodysplastic/pre-malignant Clone with Monosomy 7 with pathophysiology like that of chronic myeloid leukaemia (CML) with known genetic markers in the tumour cells...
July 7, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28682646/embryo-pooling-a-promising-strategy-for-managing-insufficient-number-of-embryos-in-preimplantation-genetic-diagnosis
#8
Xiaokun Hu, Chenhui Ding, Duoduo Zhang, Wen Zhou, Jing Wang, Yanhong Zeng, Jie Lv, Yan-Wen Xu, Can-Quan Zhou
This retrospective study evaluated the embryo pooling strategy for managing insufficient number of embryos in preimplantation genetic diagnosis (PGD) through serial vitrification of cleavage-stage embryos from consecutive cycles, and simultaneous blastocysts biopsy in combination with blastocysts obtained in ultimate fresh cycle. A retrospective analysis of the cumulative pregnancy rate of 68 patients underwent cleavage-stage embryos accumulation (Embryo Pooling Group) and 94 patients underwent one stimulation cycle (Control Group) over a 2-year period were conducted...
July 6, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28676034/cathepsin-b-inhibitor-improves-developmental-competency-and-cryo-tolerance-of-in-vitro-ovine-embryos
#9
M Pezhman, S M Hosseini, S Ostadhosseini, Sh Rouhollahi Varnosfaderani, F Sefid, M H Nasr-Esfahani
BACKGROUND: Cathepsin B is a lysosomal cysteine protease involved in apoptosis and oocytes which have lower developmental competence show higher expression of Cathepsin B. Furthermore, expression of Cathepsin B show a decreasing trend from oocyte toward blastocyst stage. RESULTS: Present study assessed the effect of cathepsin B inhibitor, E-64, on developmental competency and cryo-survival of pre-implantation ovine IVF derived embryos. Cathepsin B inhibitor was added during day 3 to 8 of development...
July 4, 2017: BMC Developmental Biology
https://www.readbyqxmd.com/read/28670673/in-vitro-application-of-pentoxifylline-preserved-ultrastructure-of-spermatozoa-after-vitrification-in-asthenozoospermic-patients
#10
Ali Nabi, Mohammad Ali Khalili, Ali Reza Talebi, Esmat Mangoli, Nahid Yari, Stefania Annarita Nottola, Selenia Miglietta, Fatemeh Taheri
PURPOSE: To evaluate the effect of in vitro application of pentoxifylline (PX) on sperm parameters and ultrastructure after vitrification in asthenozoospermic patients. MATERIALS AND METHODS: A total of 30 asthenozoospermic semen samples (aged 25-45 years) were divided into four groups before vitrification, after vitrification, control (without PX) and experimental (with PX). In experimental group, each sample was exposed for 30 min to 3.6mmol/l PX and the control group without any treatment apposing in 370C for 30 min...
July 2, 2017: Urology Journal
https://www.readbyqxmd.com/read/28670510/fertility-preservation-in-cancer-patients-in-vivo-and-in-vitro-options
#11
REVIEW
Rouhollah Fathi, Mojtaba Rezazadeh Valojerdi, Bita Ebrahimi, Farideh Eivazkhani, Mahzad Akbarpour, Leila Sadat Tahaei, Naeimeh Sadat Abtahi
Oocyte, embryo and ovarian tissue cryopreservation are being increasingly proposed for fertility preservation among cancer patients undergoing therapy to enable them to have babies after the cancer is cured. Embryo cryopreservation is not appropriate for single girls without any sperm partner and also because oocyte retrieval is an extended procedure, it is impossible in cases requiring immediate cancer cure. Thus ovarian tissue cryopreservation has been suggested for fertility preservation especial in cancer patients...
July 2017: Cell Journal
https://www.readbyqxmd.com/read/28670425/vitrification-of-human-germinal-vesicle-oocytes-before-or-after-in-vitro-maturation
#12
Evangelia Kasapi, Byron Asimakopoulos, Katerina Chatzimeletiou, Stamatios Petousis, Yannis Panagiotidis, Nikos Prapas, Nikos Nikolettos
BACKGROUND: The use of immature oocytes derived from stimulated cycles could be of great importance, particularly for urgent fertility preservation cases. The current study aimed to determine whether in vitro maturation (IVM) was more successful before or after vitrification of these oocytes. MATERIALS AND METHODS: This prospective study was performed in a private in vitro fertilization (IVF) center. We collected 318 germinal vesicle (GV) oocytes from 104 stimulated oocyte donation cycles...
July 2017: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/28667625/in-situ-imaging-of-bacterial-secretion-systems-by-electron-cryotomography
#13
Gregor L Weiss, João M Medeiros, Martin Pilhofer
The unique property of electron cryotomography (ECT) is its capability to resolve the structure of macromolecular machines in their cellular context. The integration of ECT data with high-resolution structures of purified subcomplexes and live-cell fluorescence light microscopy can generate pseudo-atomic models that lead to a mechanistic understanding across size and time scales. Recent advances in electron detection, sample thinning, data acquisition, and data processing have significantly enhanced the applicability and performance of ECT...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28658568/fragility-and-strength-in-nanoparticle-glasses
#14
Pieter van der Scheer, Ties van de Laar, Jasper van der Gucht, Dimitris Vlassopoulos, Joris Sprakel
Glasses formed from nano- and microparticles form a fascinating testing ground to explore and understand the origins of vitrification. For atomic and molecular glasses, a wide range of fragilities have been observed; in colloidal systems, these effects can be emulated by adjusting the particle softness. The colloidal glass transition can range from a superexponential, fragile increase in viscosity with increasing density for hard spheres to a strong, Arrhenius-like transition for compressible particles. However, the microscopic origin of fragility and strength remains elusive, both in the colloidal and in the atomic domains...
July 3, 2017: ACS Nano
https://www.readbyqxmd.com/read/28647785/fertility-preservation-for-trans-men-frozen-thawed-in-vitro-matured-oocytes-collected-at-the-time-of-ovarian-tissue-processing-exhibit-normal-meiotic%C3%A2-spindles
#15
S Lierman, K Tilleman, K Braeckmans, K Peynshaert, S Weyers, G T'Sjoen, P De Sutter
PURPOSES: At the moment of sex reassignment surgery (SRS), the ovarian tissue is sometimes cryopreserved as fertility preservation option for female-to-male trans men, also called trans men. During this preparation, cumulus-oocyte-complexes (COCs) can be found and in vitro matured. It is not known if these oocytes are developmentally competent. In order to use these oocytes for fertility preservation and subsequent fertilization, a normal spindle structure before and after vitrification is necessary...
June 24, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28645837/residual-ethylene-glycol-and-dimethyl-sulphoxide-concentration-in-human-ovarian-tissue-during-warming-thawing-steps-following-cryopreservation
#16
Yusuke Nakamura, Ryuichiro Obata, Noriyuki Okuyama, Nobuya Aono, Tomoko Hashimoto, Koichi Kyono
There have been 60 births after transplantation of cryopreserved ovarian tissue: 58 using the slow freezing method, and two using the vitrification method. DMSO and EG are widely used as cryoprotectants. However DMSO is a known epimutagen, and EG has been reported to be toxic in high concentrations. In this study, we measured residual DMSO and EG in ovarian tissue after vitrification and slow freezing. Cryoprotectants remained at a high concentration in the vitrified/warmed ovarian tissue just before transplantation (DMSO: 9...
June 6, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28631074/no-difference-in-mitochondrial-distribution-is-observed-in-human-oocytes-after-cryopreservation
#17
Martin Stimpfel, Eda Vrtacnik-Bokal, Irma Virant-Klun
PURPOSE: The primary aim of this study was to determine if any difference in mitochondrial distribution can be observed between fresh and cryopreserved (slow-frozen/thawed and vitrified/warmed) oocytes when oocytes are stained with Mitotracker Red CMXRos and observed under a conventional fluorescent microscope. Additionally, the influence of cryopreservation procedure on the viable rates of oocytes at different maturation stages was evaluated. METHODS: The germinal vesicle (GV) and MII oocytes were cryopreserved with slow-freezing and vitrification...
August 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/28625759/predictive-value-of-serum-hcg-concentrations-in-pregnancies-achieved-after-single-fresh-or-vitrified-warmed-blastocyst-transfer
#18
Galia Oron, Tal Shavit, Efrat Esh-Broder, Son Weon-Young, Togas Tulandi, Hananel Holzer
Possible differences between serum HCG levels in pregnancies achieved after transfer of a single fresh or a vitrified-warmed blastocyst were evaluated. Out of 1130 single blastocyst transfers resulting in positive HCG results, 789 were single fresh blastocyst transfers and 341 single vitrified-warmed blastocyst transfers. The initial serum HCG levels of 869 clinical intrauterine pregnancies were evaluated, 638 after the transfer of a single fresh blastocysts and 231 after the transfer of a single vitrified-warmed blastocysts...
May 30, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28617148/outcomes-of-ivf-cycles-coupled-with-pgs-by-acgh-of-embryos-from-donor-and-autologous-oocytes-transferred-after-vitrification-to-women-of-advanced-maternal-age
#19
Elena M Fedorova, Svetlana A Shlykova, Ksenia V Shunkina, Olga G Zaitceva, Elena N Lapina, Taras V Yanchuk, Alla S Kalugina
It is well documented that aneuploidy rate in preimplantation embryos increases with the mother's age, and at the same time the number of oocytes diminishes. Consequently, for patients of advanced maternal age two options are available to overcome these limitations: use of oocytes from young donors, or use of own oocytes coupled with preimplantation genetic screening (PGS) for 24 chromosomes. However, it is not clear which strategy might be more effective. The aim of this retrospective study was to evaluate outcomes of IVF cycles coupled with transfer of vitrified embryos from donor or autologous oocytes, both with or without PGS...
June 15, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28601150/progress-and-challenges-of-fish-sperm-vitrification-a-mini-review
#20
REVIEW
Miaomiao Xin, Mohammad Abdul Momin Siddique, Borys Dzyuba, Rafael Cuevas-Uribe, Anna Shaliutina-Kolešová, Otomar Linhart
To survive low temperature is required for a long-term storage (cryopreservation), cells should be vitrified to a state in which intracellular water is solidified without ice crystal formation. Two different approaches are described for fish sperm cryopreservation: 1) sperm conventional cryopreservation, in which extracellular water is partially crystallized and 2) sperm vitrification, in which both intra- and extra-cellular liquids are vitrified. Sperm vitrification has been applied to some fish species with limited success...
August 2017: Theriogenology
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