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Jaime Onofre, Katrien Faes, Prashant Kadam, Elena Vicini, Ans M M van Pelt, Ellen Goossens
RESEARCH QUESTION: From a clinical perspective, which parameters grant optimal cryopreservation of mouse testicular cell suspensions? DESIGN: We studied the effect of different cryopreservation rates, the addition of sugars, different vessels and the addition of an apoptotic inhibitor on the efficiency of testicular cell suspension cryopreservation. After thawing and warming, testicular cell suspensions were transplanted to recipient mice for further functional assay...
April 27, 2018: Reproductive Biomedicine Online
Hiroshi Tajimi, Tsugumi Yamazaki, Syoko Oike, Tatsuyuki Yoshida, Konosuke Okada, Masashige Kuwayama, Hitoshi Ushijima
This study was conducted to examine the utility of vitrification for bovine embryos with low-quality grade, and simple cryoprotectants dilution method for practitioners. In Experiment 1, survival of frozen embryos was compared with that of vitrified embryos using minimum volume cooling (MVC). Then, vitrified embryos were used to confirm the optimum sucrose concentration in Experiment 2. The survival rates of embryos that had been vitrified following diluted cryoprotectants with the one-step in-straw method were compared with those of fresh control embryos in Experiment 3...
May 17, 2018: Animal Science Journal, Nihon Chikusan Gakkaihō
Nolan J Tiersch, William M Childress, Terrence R Tiersch
Vitrification is a method of cryopreservation that freezes samples rapidly, while forming an amorphous solid ("glass"), typically in small (μL) volumes. The goal of this project was to create, by three-dimensional (3D) printing, open vitrification devices based on an elliptical loop that could be efficiently used and stored. Vitrification efforts can benefit from the application of 3D printing, and to begin integration of this technology, we addressed four main variables: thermoplastic filament type, loop length, loop height, and method of loading...
May 16, 2018: Zebrafish
Amir Arav, Pasquale Patrizio
No abstract text is available yet for this article.
May 10, 2018: Journal of Assisted Reproduction and Genetics
Akram Hosseini, Mohammad Ali Khalili, Ali Reza Talebi, Azam Agha-Rahimi, Saeed Ghasemi-Esmailabad, Bryan Woodward, Nahid Yari
Cryopreservation and subsequent survival of semen samples with low numbers of human spermatozoa in assisted reproductive technology (ART) facilities can be challenging. The aim of this study was to compare the quality of warmed human spermatozoa following vitrification using direct submerging (DS) in liquid nitrogen (LN2 ) or with LN2 vapour (V). Normozoospermic ejaculates were prepared by the swim-up technique and the motile sperm fraction was divided into three groups: (i) fresh (control); (ii) DS methods in LN2 (DS Group); or (iii) cryopreserved in V (Group V)...
May 11, 2018: Human Fertility: Journal of the British Fertility Society
Michael Elbaum
Electron tomography provides a detailed view into the 3D structure of biological cells and tissues. Physical fixation by vitrification of the aqueous medium provides the most faithful preservation of biological specimens in the native, fully hydrated state. Cryo-microscopy is challenging, however, because of the sensitivity to electron irradiation and due to the weak electron scattering of organic material. Tomography is even more challenging because of the dependence on multiple exposures of the same area...
May 11, 2018: Advanced Materials
Muhamad Abidalla, Pio F Roversi
Cryopreservation of viable cells and cell materials is being developed for biological and biopharmaceutical applications. The inhibition of ice formation during the cooling and warming phase of vitrified living biological samples is important for their survival. The tendency to form glasses (glass transition temperature, Tg ) upon cooling in the vitrification solution and the stability of the amorphous state upon warming to determine the critical cooling rate (Vccr ) and critical warming rates (Vcwr ) are evaluated...
May 10, 2018: Biopreservation and Biobanking
Emily Schofield, Edward P Jones, Viswambharan Sarasan
BACKGROUND: Orchids are under threat from human activities and climate change, with populations limited to small geographic hotspots. This makes them ideal candidates for ex situ conservation. Orchid seeds are desiccation tolerant, but often have poor longevity in seed banks and cryopreservation of orchid protocorms is complex and expensive. Therefore, simple methods for large-scale storage programs are essential to store orchid seeds of different life forms. Seeds of five species representing epiphytic, lithophytic and terrestrial orchids from the Central Highlands of Madagascar were studied to find a simple and effective system of cryopreservation...
May 9, 2018: Botanical Studies (Taipei, Taiwan)
H S Youm, J R Choi, D Oh, Y H Rho
BACKGROUND: T he most commonly used methods for the cryopreservation of oocytes and embryos are vitrification and slow freezing. OBJECTIVE: The aim of this study was to to investigate whether there are differences in survival, in vitro maturation (IVM), and fertilization rates between cryopreserved immature oocytes, especially germinal vesicle (GV)-stage human oocytes, following vitrification and slow freezing. MATERIALS AND METHODS: A literature search was performed using the MEDLINE, Cochrane Central Register of Controlled Trials, and Embase databases...
November 2017: Cryo Letters
K S Ping, R R Poobathy, R Zakaria, S Subramaniam
  BACKGROUND: Conservation of commercially important ornamental plants is important to maintain its unique beauty to cater the market demands. OBJECTIVE: The main objective is to develop an efficient cryopreservation technique for Aranda Broga Blue orchid PLBs using droplet-vitrification method. MATERIALS AND METHODS: Several critical factors in cryopreservation were accessed such as preculture concentrations and durations, choice of vitrification solutions, two-step or three-step vitrification, growth recovery medium and PVS2 exposure duration...
July 2017: Cryo Letters
E J Hoekman, K E Tucker, C Am Jansen, C G Hilders
BACKGROUND: Oocyte vitrification is important for fertility preservation. There are debates, however, surrounding the components of the procedure itself. OBJECTIVE: before starting a vitrification program, we decided to (1) determine if blastocysts derived from previously frozen mouse embryos would be suitable to practice vitrification and (2) to analyze the factors that contributed to improving our "learning curve". MATERIALS AND METHODS: 58 expanded blastocysts cultured from commercially available frozen 1-cell mouse embryos (B6D2F1 x B6C3F1) were used...
March 2018: Cryo Letters
S S Sahraei, M Shahhoseini, B Movaghar
BACKGROUND: OBJECTIVE: This paper reports results from studies on modifications of histone marks in transcriptional regulatory regions of H19, Igf2 and Mest genes in vitrified-warmed and cultured mouse embryos. MATERIALS AND METHODS: Non vitrified and vitrified-warmed 8-cell embryos were cultured in Ham's F10+10% BSA into blastocyst stage in two experimental groups. Gene expression level was assessed by quantitative PCR and histone modifications were evaluated by ChIP assay method...
March 2018: Cryo Letters
Y Wang, X M Zhou, C J Jiang, Y T Yu
BACKGROUND: The microchannel heat exchange system has several advantages and can be used to enhance heat transfer for vitrification. OBJECTIVE: To evaluate the microchannel cooling method and to analyze the effects of key parameters such as channel structure, flow rate and sample size. MATERIALS AND METHODS: A computational flow dynamics model is applied to study the two-phase flow in microchannels and its related heat transfer process. The fluid-solid coupling problem is solved with a whole field solution method (i...
January 2018: Cryo Letters
V H Do, S Watson, S Catt, A W Taylor-Robinson
  BACKGROUND: In order to thaw slow-cooled bovine embryos it is standard practice to draw out permeating cryoprotectants by passing embryos through successively decreasing osmotic solutions. However, recently it has been suggested that sucrose may not be needed in the warming media. OBJECTIVE: The aim of this experiment was to compare the effect of warming media prepared with or without the inclusion of sucrose on the survival and hatching capacity of vitrified in vitro-derived bovine embryos...
September 2017: Cryo Letters
J Stock, A Semula, M Nagel, H Mock, E R Joachim Keller
  BACKGROUND: The use of the model plant Arabidopsis could be a valuable tool to elucidate the basic mechanisms involved in plant cryopreservation. OBJECTIVE: A simple and powerful protocol, independent of Arabidopsis genotypes, was established using a PVS2 protocol. MATERIALS AND METHODS: Two PVS2 (a and b), one PVS3 droplet-vitrification and one DMSO droplet-freezing protocol were tested with alternating temperatures during the growing phase of donor plants...
September 2017: Cryo Letters
Avci Berrin, Kasapoglu Isıl, Ata Baris, Kuspinar Goktan, Saribal Seda, Uncu Gurkan
The goal of this retrospective cohort study was to compare survival, implantation, clinical and ongoing pregnancy rates between blastocysts that were vitrified on post-fertilization days 5, 6 and 7. Before vitrification, blastocysts were evaluated in terms of morphology and blastocyst expansion, inner cell mass and trophectoderm quality. They were thawed and transfered in a subsequent artificial cycle. Embryo implantation rates were 39%, 25% and 25% for blastocysts that were vitrified on days 5, 6, and 7, respectively (p = 0...
May 3, 2018: Human Fertility: Journal of the British Fertility Society
Farkhondeh Pooyanfar, Tahereh Foroutan, Mojtaba Dashtizad
Although the rate of blastocysts implantation of embryos is higher than previous stages but their survival rate is lower than them, which could be attributed to the completely filled blastocoel cavity with liquid and increased possibility of the formation of ice crystals. This liquid could prevent the penetration of cryoprotecting materials into the embryos. In this study, we reduced the volume of blastocoel before vitrification and compared survival rate and quality of in vitro embryos through klf4 gene expression with control group...
2018: Veterinary Research Forum
Timothy J Tyree, Ritwik Dan, Robert E Thorne
The glass-phase densities at T = 77 K of aqueous solutions of the common cryoprotective agents (CPAs) methanol, ethanol, 2-propanol, glycerol, 2-methyl-2,4-pentanediol (MPD), ethylene glycol, polyethylene glycol 200 and polypropylene glycol 425 were measured as a function of CPA concentration. Individual drops with volumes as small as ∼65 pl were rapidly cooled to achieve the glass phase, and their densities at T = 77 K were determined by cryoflotation. These densities were used to determine the glass-phase electron density of each solution and its volume thermal contraction between room temperature and 77 K...
May 1, 2018: Acta Crystallographica. Section D, Structural Biology
Keiji Mochida
In conventional vitrification methods, the embryos are vitrified under considerable supercooling (i.e., under nonequilibrium conditions). This protocol is a refinement of newer equilibrium vitrification methods. The equilibrium vitrification solution contains a high concentration of cryoprotectants that increase its osmolality and allows vitrified embryos to survive not only during storage in liquid nitrogen (LN2 ) at -196°C but also during temporary holding at -80°C (for at least 5 mo). This high-osmolality vitrification (HOV) method is as simple as conventional vitrification and provides essentially the same high postwarming survival rate...
May 1, 2018: Cold Spring Harbor Protocols
Lucky Sekhon, Joseph A Lee, Eric Flisser, Alan B Copperman, Daniel Stein
RESEARCH QUESTION: Does vitrification and warming affect live birth rate, infant birth weight and timing of delivery? DESIGN: Retrospective, cohort study comparing outcomes of donor oocyte recipient fresh (n = 25) versus vitrified (n = 86) euploid blastocyst transfers; donor oocyte recipient singleton live births from fresh (n = 100) versus vitrified (n = 102) single embryo transfers (SET); and autologous vitrified euploid SET (n = 1760) (cryostored 21-1671 days)...
April 21, 2018: Reproductive Biomedicine Online
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