keyword
MENU ▼
Read by QxMD icon Read
search

islet 3d

keyword
https://www.readbyqxmd.com/read/29322773/sers-based-label-free-insulin-detection-at-physiological-concentrations-for-analysis-of-islet-performance
#1
Hyunjun Cho, Shailabh Kumar, Daejong Yang, Sagar R Vaidyanathan, Kelly Woo, Ian Garcia, Hao Jan Shue, Youngzoon Yoon, Kevin Ferreri, Hyuck Choo
Label-free optical detection of insulin would allow in vitro assessment of pancreatic cell functions in their natural state and expedite diabetes-related clinical research and treatment, however no existing method has met these criteria at physiological concentrations. Using spatially-uniform 3D gold-nanoparticle sensors, we have demonstrated surface-enhanced Raman sensing of insulin in the secretions from human pancreatic islets under low and high glucose environments without the use of labels such as antibodies or aptamers...
January 11, 2018: ACS Sensors
https://www.readbyqxmd.com/read/29310936/the-rhogap-stard13-controls-insulin-secretion-through-f-actin-remodeling
#2
Heike Naumann, Thomas Rathjen, Matthew N Poy, Francesca M Spagnoli
OBJECTIVE: Actin cytoskeleton remodeling is necessary for glucose-stimulated insulin secretion in pancreatic β-cells. A mechanistic understanding of actin dynamics in the islet is paramount to a better comprehension of β-cell dysfunction in diabetes. Here, we investigate the Rho GTPase regulator Stard13 and its role in F-actin cytoskeleton organization and islet function in adult mice. METHODS: We used Lifeact-EGFP transgenic animals to visualize actin cytoskeleton organization and dynamics in vivo in the mouse islets...
December 28, 2017: Molecular Metabolism
https://www.readbyqxmd.com/read/29298810/highly-angiogenic-non-thrombogenic-bone-marrow-mononuclear-cells-derived-spheroids-in-intraportal-islet-transplantation
#3
Bae Jun Oh, Sang-Man Jin, Yoonha Hwang, Jin Myung Choi, Han-Sin Lee, Gyuri Kim, Geunsoo Kim, Hyo Jun Park, Pilhan Kim, Sung Joo Kim, Jae Hyeon Kim
Highly angiogenic bone marrow mononuclear cells-derived spheroids (BM-spheroids), formed by selective proliferation of the CD31+CD14+CD34+ monocyte subset via 3D culture, had robust angiogenetic capacity in rodent syngeneic renal subcapsular islet transplantation. We wondered whether the efficacy of BM-spheroids could be demonstrated in clinically-relevant intraportal islet transplantation models without increasing the risk of portal thrombosis. The thrombogenic potential of intraportally infused BM-spheroids was compared with that of mesenchymal stromal cells (MSCs) and MSC-spheroids...
January 3, 2018: Diabetes
https://www.readbyqxmd.com/read/29283208/microphysiological-analysis-platform-of-pancreatic-islet-%C3%AE-cell-spheroids
#4
Sang Hun Lee, SoonGweon Hong, Jihwan Song, Byungrae Cho, Esther J Han, Sravani Kondapavulur, Dongchoul Kim, Luke P Lee
The hallmarks of diabetics are insufficient secretion of insulin and dysregulation of glucagon. It is critical to understand release mechanisms of insulin, glucagon, and other hormones from the islets of Langerhans. In spite of remarkable advancements in diabetes research and practice, robust and reproducible models that can measure pancreatic β-cell function are lacking. Here, a microphysiological analysis platform (MAP) that allows the uniform 3D spheroid formation of pancreatic β-cell islets, large-scale morphological phenotyping, and gene expression mapping of chronic glycemia and lipidemia development is reported...
December 28, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29241367/differentiation-of-conjunctiva-mesenchymal-stem-cells-into-secreting-islet-beta-cells-on-plasma-treated-electrospun-nanofibrous-scaffold
#5
Samad Nadri, Ghasem Barati, Hossein Mostafavi, Abdolreza Esmaeilzadeh, Seyed Ehsan Enderami
Transplantation of stem cells using biocompatible nanofibrous scaffolds is a promising therapeutic method for treating Diabetic Mellitus. The aim of this study was to derive insulin-producing cells (IPCs) from conjunctiva-derived mesenchymal stem cell (CJMSCs) and to compare the functionality of differentiated IPCs in a three-dimensional (3D) culture with 2D. Furthermore, the effects of hydrophobicity of scaffold on IPCs differentiation were examined. Scanning electron microscopy (SEM), quantitative real times PCR (qPCR), Immunostaining and flow cytometry were used to analyze fabricated scaffold and the presence of IPCs...
December 14, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29181780/3d-models-of-insulin-producing-%C3%AE-cells-from-primary-islet-cells-to-stem-cell-derived-islets
#6
REVIEW
Diana Ribeiro, Alexander J Kvist, Pernilla Wittung-Stafshede, Ryan Hicks, Anna Forslöw
There is a need for physiologically relevant assay platforms to provide functionally relevant models of diabetes, to accelerate the discovery of new treatment options and boost developments in drug discovery. In this review, we compare several 3D-strategies that have been used to increase the functional relevance of ex vivo human primary pancreatic islets and developments into the generation of stem cell derived pancreatic beta-cells (β-cells). Special attention will be given to recent approaches combining the use of extracellular matrix (ECM) scaffolds with pancreatic molecular memory, which can be used to improve yield and functionality of in vitro stem cell-derived pancreatic models...
November 27, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/29150935/generation-of-insulin-producing-cells-from-human-adipose-derived-mesenchymal-stem-cells-on-pva-scaffold-by-optimized-differentiation-protocol
#7
Seyed Ehsan Enderami, Masoud Soleimani, Yousef Mortazavi, Samad Nadri, Ali Salimi
The studies have been done on patient-specific human adipose-derived from mesenchymal stem cells (hADSCs) like a series of autologous growth factors and nanofibrous scaffolds (3D culture) will probably have many benefits for regenerative medicine in type 1 diabetes mellitus (TIDM) patients in the future. For this purpose, we established a polyvinyl alcohol (PVA) scaffold and a differentiation protocol by adding platelet-rich plasma (PRP) that induces the hADSCs into insulin-producing cells (IPCs). The Characteristics of the derived IPCs in 3D culture were compared with conventional culture (2D) groups evaluated at the mRNA and protein levels...
November 18, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29142323/nkx6-1-decline-accompanies-mitochondrial-dna-reduction-but-subtle-nucleoid-size-decrease-in-pancreatic-islet-%C3%AE-cells-of-diabetic-goto-kakizaki-rats
#8
Tomáš Špaček, Vojtěch Pavluch, Lukáš Alán, Nikola Capková, Hana Engstová, Andrea Dlasková, Zuzana Berková, František Saudek, Petr Ježek
Hypertrophic pancreatic islets (PI) of Goto Kakizaki (GK) diabetic rats contain a lower number of β-cells vs. non-diabetic Wistar rat PI. Remaining β-cells contain reduced mitochondrial (mt) DNA per nucleus (copy number), probably due to declining mtDNA replication machinery, decreased mt biogenesis or enhanced mitophagy. We confirmed mtDNA copy number decrease down to <30% in PI of one-year-old GK rats. Studying relations to mt nucleoids sizes, we employed 3D superresolution fluorescent photoactivable localization microscopy (FPALM) with lentivirally transduced Eos conjugate of mt single-stranded-DNA-binding protein (mtSSB) or transcription factor TFAM; or by 3D immunocytochemistry...
November 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29117231/human-pancreatic-islet-derived-extracellular-vesicles-modulate-insulin-expression-in-3d-differentiating-ipsc-clusters
#9
Diana Ribeiro, Eva-Marie Andersson, Nikki Heath, Anette Persson-Kry, Richard Collins, Ryan Hicks, Niek Dekker, Anna Forslöw
It has been suggested that extracellular vesicles (EVs) can mediate crosstalk between hormones and metabolites within pancreatic tissue. However, the possible effect of pancreatic EVs on stem cell differentiation into pancreatic lineages remains unknown. Herein, human islet-derived EVs (h-Islet-EVs) were isolated, characterized and subsequently added to human induced pluripotent stem cell (iPSC) clusters during pancreatic differentiation. The h-islet-EVs had a mean size of 117±7 nm and showed positive expression of CD63 and CD81 EV markers as measured by ELISA...
2017: PloS One
https://www.readbyqxmd.com/read/29101017/enhanced-oxygen-permeability-in-membrane-bottomed-concave-microwells-for-the-formation-of-pancreatic-islet-spheroids
#10
GeonHui Lee, Yesl Jun, HeeYeong Jang, Junghyo Yoon, JaeSeo Lee, MinHyung Hong, Seok Chung, Dong-Hwee Kim, SangHoon Lee
Oxygen availability is a critical factor in regulating cell viability that ultimately contributes to the normal morphogenesis and functionality of human tissues. Among various cell culture platforms, construction of 3D multicellular spheroids based on microwell arrays has been extensively applied to reconstitute in vitro human tissue models due to its precise control of tissue culture conditions as well as simple fabrication processes. However, an adequate supply of oxygen into the spheroidal cellular aggregation still remains one of the main challenges to producing healthy in vitro spheroidal tissue models...
October 31, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29095600/cofibrillization-of-pathogenic-and-functional-amyloid-proteins-with-gold-nanoparticles-against-amyloidogenesis
#11
Ibrahim Javed, Yunxiang Sun, Jozef Adamcik, Bo Wang, Aleksandr Kakinen, Emily H Pilkington, Feng Ding, Raffaele Mezzenga, Thomas P Davis, Pu Chun Ke
Biomimetic nanocomposites and scaffolds hold the key to a wide range of biomedical applications. Here we show, for the first time, a facile scheme of cofibrillizing pathogenic and functional amyloid fibrils via gold nanoparticles (AuNPs) and their applications against amyloidogenesis. This scheme was realized by β-sheet stacking between human islet amyloid polypeptide (IAPP) and the β-lactoglobulin "corona" of the AuNPs, as revealed by transmission electron microscopy, 3D atomic force microscopy, circular dichroism spectroscopy, and molecular dynamics simulations...
November 14, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28917147/combined-in-silico-approaches-for-the-identification-of-novel-inhibitors-of-human-islet-amyloid-polypeptide-hiapp-fibrillation
#12
Palak Patel, Krupali Parmar, Vivek K Vyas, Dhaval Patel, Mili Das
Human islet amyloid polypeptide (hIAPP) is a natively unfolded polypeptide hormone of glucose metabolism, which is co-secreted with insulin by the β-cells of the pancreas. In patients with type 2 diabetes, IAPP forms amyloid fibrils because of diabetes-associated β-cells dysfunction and increasing fibrillation, in turn, lead to failure of secretory function of β-cells. This provides a target for the discovery of small organic molecules against protein aggregation diseases. However, the binding mechanism of these molecules with monomers, oligomers and fibrils to inhibit fibrillation is still an open question...
September 6, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28864913/pancreatic-neuro-insular-network-in-young-mice-revealed-by-3d-panoramic-histology
#13
Shiue-Cheng Tang, Chia-Ning Shen, Pei-Yu Lin, Shih-Jung Peng, Hung-Jen Chien, Ya-Hsien Chou, Chester E Chamberlain, Pankaj J Pasricha
AIMS/HYPOTHESIS: It has been proposed that the neuro-insular network enables rapid, synchronised insulin secretion. However, to date, acquiring the pancreatic tissue map to study the neural network remains a challenging task as there is a lack of feasible approaches for large-scale tissue analysis at the organ level. Here, we have developed 3-dimensional (3D) panoramic histology to characterise the pancreatic neuro-insular network in young mice. METHODS: Pancreases harvested from young wild-type B6 mice (3 and 8 weeks old) and db/db mice (3 weeks old; db/db vs db/+) were used to develop 3D panoramic histology...
September 1, 2017: Diabetologia
https://www.readbyqxmd.com/read/28852792/human-pancreatic-neuro-insular-network-in-health-and-fatty-infiltration
#14
Shiue-Cheng Tang, Luc Baeyens, Chia-Ning Shen, Shih-Jung Peng, Hung-Jen Chien, David W Scheel, Chester E Chamberlain, Michael S German
AIMS/HYPOTHESIS: Identification of a pancreatic neuro-insular network in mice suggests that a similar integration of islets and nerves may be present in the human pancreas. To characterise the neuro-insular network and the intra-pancreatic ganglia in a clinically related setting, we examined human pancreases in health and with fatty infiltration via 3-dimensional (3D) histology and compared the human pancreatic microenvironment with its counterpart in mice. METHODS: Human pancreatic specimens from individuals with normal BMI, high BMI (≥ 25) and type 2 diabetes were used to investigate the neuro-insular network...
August 29, 2017: Diabetologia
https://www.readbyqxmd.com/read/28734022/3d-printed-vascularized-device-for-subcutaneous-transplantation-of-human-islets
#15
Marco Farina, Andrea Ballerini, Daniel W Fraga, Eugenia Nicolov, Matthew Hogan, Danilo Demarchi, Francesco Scaglione, Omaima M Sabek, Philip Horner, Usha Thekkedath, Osama A Gaber, Alessandro Grattoni
Transplantation of pancreatic islets or stem cell derived insulin secreting cells is an attractive treatment strategy for diabetes. However, islet transplantation is associated with several challenges including function-loss associated with dispersion and limited vascularization as well as the need for continuous immunosuppression. To overcome these limitations, here we present a novel 3D printed and functionalized encapsulation system for subcutaneous engraftment of islets or islet like cells. The devices were 3D printed with polylactic acid and the surfaces treated and patterned to increase the hydrophilicity, cell attachment, and proliferation...
July 22, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28691373/stromal-vascular-fraction-loaded-silk-fibroin-mats-effectively-support-the-survival-of-diabetic-mice-after-pancreatic-islet-transplantation
#16
Sara Perteghella, Barbara Vigani, Luca Mastracci, Federica Grillo, Barbara Antonioli, Marta Galuzzi, Marta Cecilia Tosca, Barbara Crivelli, Stefania Preda, Giuseppe Tripodo, Mario Marazzi, Theodora Chlapanidas, Maria Luisa Torre
The aim of this study is to assess whether stromal vascular fraction (SVF)-soaked silk fibroin nonwoven mats (silk-SVF) can preserve the functionality of encapsulated pancreatic endocrine cells (alginate-PECs) after transplantation in the subcutaneous tissue of diabetic mice. Silk scaffolds are selected to create an effective 3D microenvironment for SVF delivery in the subcutaneous tissue before diabetes induction: silk-SVF is subcutaneously implanted in the dorsal area of five healthy animals; after 15 d, mice are treated with streptozotocin to induce diabetes and then alginate-PECs are implanted on the silk-SVF...
September 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/28571932/detection-of-the-relatively-slow-growing-propionibacterium-acnes-in-seven-matrices-of-blood-components-and-advanced-therapeutical-medicinal-products
#17
Nicole Arlt, Remo Rothe, Thomas Juretzek, Heidrun Peltroche, Torsten Tonn, Rainer Moog
BACKGROUND: Relatively slow-growing bacteria like Propionibacterium acnes represent a challenge for quality control investigations in sterility release testing of blood components and advanced therapeutic medicinal products (ATMPs). METHODS: A convenient validation with 7 matrices was performed using buffy coat, stem cells, islet cells, natural killer cells, red blood cells, platelets and plasma in the microbial detection system Bact/Alert(®)3D incubator. All matrix samples were spiked twofold with Propionibacterium acnes with approximately 50 colony forming units (CFUs) per bottle in iAST and iNST culture bottles for 14days using a multishot bioball...
May 19, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28540469/a-3d-microfluidic-perfusion-system-made-from-glass-for-multiparametric-analysis-of-stimulus-secretioncoupling-in-pancreatic-islets
#18
Torben Schulze, Kai Mattern, Eike Früh, Lars Hecht, Ingo Rustenbeck, Andreas Dietzel
Microfluidic perfusion systems (MPS) are well suited to perform multiparametric measurements with small amounts of tissue to function as an Organ on Chip device (OOC). Such microphysiolgical characterization is particularly valuable in research on the stimulus-secretion-coupling of pancreatic islets. Pancreatic islets are fully functional competent mini-organs, which serve as fuel sensors and transduce metabolic activity into rates of hormone secretion. To enable the simultaneous measurement of fluorescence and oxygen consumption we designed a microfluidic perfusion system from borosilicate glass by 3D femtosecond laser ablation...
September 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28360105/demethylation-of-induced-pluripotent-stem-cells-from-type-1-diabetic-patients-enhances-differentiation-into-functional-pancreatic-%C3%AE-cells
#19
COMPARATIVE STUDY
Gohar S Manzar, Eun-Mi Kim, Nicholas Zavazava
Type 1 diabetes (T1D) can be managed by transplanting either the whole pancreas or isolated pancreatic islets. However, cadaveric pancreas is scarcely available for clinical use, limiting this approach. As such, there is a great need to identify alternative sources of clinically usable pancreatic tissues. Here, we used induced pluripotent stem (iPS) cells derived from patients with T1D to generate glucose-responsive, insulin-producing cells (IPCs) via 3D culture. Initially, T1D iPS cells were resistant to differentiation, but transient demethylation treatment significantly enhanced IPC yield...
August 25, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28347646/allogeneic-islet-cells-implant-on-poly-l-lactide-matrix-to-reduce-hyperglycaemia-in-streptozotocin-induced-diabetic-rat
#20
Siufui Hendrawan, Irawan Yusuf, Mochammad Hatta, Makbul Aman, Ilhamjaya Patellongi, Andreas L Serra, Gatot Lawrence, Ursula Weber, Barlian Sutedja, Hans U Baer
OBJECTIVE: To demonstrate the effects of allogeneic islet cell matrix implants for glycaemic control in rats with induced diabetes. METHOD: Sprague-Dawley rats were used as allogeneic donors of islet cells. Cells were seeded on three-dimensional proprietary poly-(l-lactide) matrices. Animals were rendered diabetic and a week later a matrix seeded with islet cells (IMI group) or a control matrix (placebo group) was implanted in the small bowel mesentery. Blood glucose levels were measured weekly for 12 weeks...
March 1, 2017: Pancreatology: Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]
keyword
keyword
84266
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"