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https://www.readbyqxmd.com/read/29045433/integrated-mrna-and-microrna-transcriptome-analyses-reveal-regulation-of-thermal-acclimation-in-gymnocypris-przewalskii-a-case-study-in-tibetan-schizothoracine-fish
#1
Cunfang Zhang, Chao Tong, Fei Tian, Kai Zhao
Environmental acclimation is important episode in wildlife occupation of the high-altitude Tibetan Plateau (TP). Transcriptome-wide studies on thermal acclimation mechanism in fish species are rarely revealed in Tibetan Plateau fish at high altitude. Thus, we used mRNA and miRNA transcriptome sequencing to investigate regulation of thermal acclimation in larval Tibetan naked carp, Gymnocypris przewalskii. We first remodeled the regulation network of mRNA and miRNA in thermal acclimation, and then identified differential expression of miRNAs and target mRNAs enriched in metabolic and digestive pathways...
2017: PloS One
https://www.readbyqxmd.com/read/29044045/emergence-of-carp-edema-virus-cev-and-its-significance-to-european-common-carp-and-koi-cyprinus-carpio
#2
REVIEW
K Way, O Haenen, D Stone, M Adamek, S M Bergmann, L Bigarré, N Diserens, M El-Matbouli, M C Gjessing, V Jung-Schroers, E Leguay, M Matras, N J Olesen, V Panzarin, V Piačková, A Toffan, N Vendramin, T Vesel, T Waltzek
Carp edema virus disease (CEVD), also known as koi sleepy disease, is caused by a poxvirus associated with outbreaks of clinical disease in koi and common carp Cyprinus carpio. Originally characterised in Japan in the 1970s, international trade in koi has led to the spread of CEV, although the first recognised outbreak of the disease outside of Japan was not reported until 1996 in the USA. In Europe, the disease was first recognised in 2009 and, as detection and diagnosis have improved, more EU member states have reported CEV associated with disease outbreaks...
October 18, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/29038001/g0s2a1-g0-g1-switch-gene-2a1-is-downregulated-by-tnf-%C3%AE-in-grass-carp-ctenopharyngodon-idellus-hepatocytes-through-ppar%C3%AE-inhibition
#3
Jian Sun, Zhou Yang, Xiao-Chen Shi, Hong Ji, Zhen-Yu Du, Li-Qiao Chen
G0/G1 switch gene 2 plays an important role in the regulation of lipolysis in mammals, but little is known about its gene (G0S2) structure and function in fish. In the present study, two genes, G0S2a and G0S2b were isolated and characterized from grass carp Ctenopharyngodon idella, which encode peptides of 111 and 84 amino acids, respectively. Moreover, alternative multiple exon usage resulted in a significant variation in the 5'-region of G0S2a transcripts yielding two isoforms (G0S2a1 and G0S2a2). Phylogenetic and synteny analyses indicated that G0S2a and G0S2b could have originated from the teleost-specific genome duplication event...
October 13, 2017: Gene
https://www.readbyqxmd.com/read/29037731/sequential-extraction-of-gel-forming-proteins-collagen-and-collagen-hydrolysate-from-gutted-silver-carp-hypophthalmichthys-molitrix-a-biorefinery-approach
#4
Mehdi Abdollahi, Masoud Rezaei, Ali Jafarpour, Ingrid Undeland
Collagen and collagen hydrolysate (CH) was recovered from the bone and skin containing sediment residue emerging during pH-shift-based protein isolation from silver carp. Hydrolysis resulted in higher yield (15.1-15.4%) compared to collagen isolation by acid or pepsin (3.1-5.9%) (p<0.05). Isolated collagens were characterized as type I and maintained their triple-helical structure, confirmed by SDS-PAGE and FTIR. Pepsin-hydrolysis and sequential hydrolysis by pepsin and trypsin hydrolyzed all heavy molecular weight chains of collagen but sequential hydrolysis yielded higher degree of hydrolysis...
March 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29037708/the-effects-of-edible-chitosan-based-coatings-on-flavor-quality-of-raw-grass-carp-ctenopharyngodon-idellus-fillets-during-refrigerated-storage
#5
Dawei Yu, Yanshun Xu, Joe M Regenstein, Wenshui Xia, Fang Yang, Qixing Jiang, Bin Wang
This study investigated the effects of chitosan-based coatings on flavor retention of refrigerated grass carp fillets by using various indicators: free amino acids (FAA), nucleotides, trimethylamine (TMA), volatile profile, sensory quality, and electronic nose analysis. The results indicated that chitosan-based coatings contributed to the significant reduction of off-flavor compounds, such as TMA, hypoxanthine (Hx) and histidine, and accumulation of inosine monophosphate (IMP) and umami-associated FAA. GC-MS analysis showed 23 volatile organic compounds, including many C5-C9 aldehydes and alcohols in the fresh fillets...
March 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29037642/rebound-boots-change-lower-limb-muscle-activation-and-kinematics-during-different-fitness-exercises
#6
Mateus Rossato, Rodolfo André Dellagrana, Juliane Cristine Lopes Dos Santos, Felipe P Carpes, Rodrigo Ghedini Gheller, De Angelys de Ceselles Seixas da Silva, Ewertton de Souza Bezerra, João Otacílio Libardoni Dos Santos
The purpose of this study was to evaluate electromyography and kinematic parameters of the lower limbs using rebound boots (RB) and barefoot during a gym workout. This information can be helpful to practitioners to schedule rehabilitation and training programs. Ten women (25 ± 9 years) volunteered for the study; inclusion criteria were as follows: subjects must have experienced the use of RB and the analyzed exercises for at least 6 months, and have no previous injuries in the lower limbs. Seven exercises were performed for 30 s with the RB and subsequently barefoot...
October 2017: Journal of Bodywork and Movement Therapies
https://www.readbyqxmd.com/read/29035631/bioconcentration-and-half-life-of-quinalphos-pesticide-in-rice-fish-integration-system-in-the-mekong-delta-vietnam
#7
Nguyen Quoc Thinh, Tran Minh Phu, Caroline Douny, Nguyen Thanh Phuong, Do Thi Thanh Huong, Patrick Kestemont, Marie-Louise Scippo
In order to determine the distribution and enable the elimination of quinalphos, a popular active pesticide compound used in the Mekong Delta, an experiment was set up in a rice-fish integration system in Can Tho City, Vietnam. Fish was stocked into the field when the rice was two-months old. Quinalphos was applied twice in doses of 42.5 g per 1000 m(2). Water, fish and sediment samples were collected at time intervals and analyzed by a Gas Chromatography Electron Capture Detector system. The results show that quinalphos residues in fish muscles were much higher than those of the water and the bioconcentration factor (logBCF) was above 2 for the fish...
October 16, 2017: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/29032307/biomechanical-properties-of-different-techniques-used-in-vitro-for-suturing-mid-substance-achilles-tendon-ruptures
#8
Carlos De la Fuente, Carlos Cruz-Montecinos, Helen L Schimidt, Hugo Henríquez, Sebastián Ruidiaz, Felipe P Carpes
BACKGROUND: The Dresden technique preserves the paratenon during Achilles tendon repair and may improve the plantarflexor mechanism when combined with mobilization during early rehabilitation. However, the surgical repair design for Achilles tendon ruptures can affect rates of re-rupture or lengthening. Therefore, the aim of this study was to determine the biomechanical properties of the Krackow, Double-Kessler, Double-Dresden, and Triple-Dresden techniques used for repairing mid-substance Achilles tendon ruptures during cyclical and maximum traction...
October 9, 2017: Clinical Biomechanics
https://www.readbyqxmd.com/read/29032082/effects-of-triclosan-on-hormones-and-reproductive-axis-in-female-yellow-river-carp-cyprinus-carpio-potential-mechanisms-underlying-estrogen-effect
#9
Fan Wang, Xiangmeng Guo, Wanguang Chen, Yaowen Sun, Chaojie Fan
Triclosan (TCS), a member of the class of compounds called pharmaceutical and personal care products (PPCPs), is a broad antibacterial and antifungal agent found in a lot of consumer products. However, TCS hormone effect mechanism in teleost female fish is not clear. Female Yellow River carp (Cyprinus carpio) were exposed to 1/20, 1/10 and 1/5 LC50 TCS (96h LC50 of TCS to carp) under semi-static conditions for 42days. Vitellogenin (Vtg), 17β-estradiol (E2), testosterone(T), estrogen receptor (Er), gonadotropin (GtH), and gonadotropin-releasing hormone (GnRH) levels were measured by enzyme-linked immunosorbent assay (ELISA)...
October 12, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29031548/green-tea-protects-against-memory-deficits-related-to-maternal-deprivation
#10
Jefferson Menezes, Ben-Hur Neves, Mauren Souza, Pâmela Billig Mello-Carpes
Maternal deprivation (MD) in early life affects the development of the brain, causing cognitive losses in adulthood. Oxidative imbalance may be one of the factors that trigger these deficits. Therapies with antioxidant components, like green tea from Camellia sinensis (GT) has been used to treat or prevent memory deficits in a variety of conditions related to oxidative stress. Here we demonstrate that memory deficits caused by MD can be prevented by GT antioxidant activity in hippocampus. Pregnant female rats were used...
October 12, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/29031051/17%C3%AE-estradiol-induces-cyto-genotoxicity-on-blood-cells-of-common-carp-cyprinus-carpio
#11
Luis Orozco-Hernández, Adriana Andrea Gutiérrez-Gómez, Nely SanJuan-Reyes, Hariz Islas-Flores, Sandra García-Medina, Marcela Galar-Martínez, Octavio Dublán-García, Reyna Natividad, Leobardo Manuel Gómez-Oliván
17β-Estradiol, a natural hormone present at high concentrations in aquatic ecosystems, affects and modifies endocrine function in animals. In recent years research workers have expressed concern over its potential effects on aquatic organisms; however, little is known about its capacity to induce genetic damage or the pro-apoptotic effects of such damage on fish. Therefore, this study aimed to evaluate 17β-estradiol-induced cyto-genotoxicity in blood cells of the common carp Cyprinus carpio exposed to different concentrations (1 ng, 1 μg and 1 mg L(-1))...
October 6, 2017: Chemosphere
https://www.readbyqxmd.com/read/29029341/an-increased-concentration-of-receptor-activator-of-nuclear-factor-kappa-b-ligand-pre-dates-the-onset-of-rheumatoid-arthritis
#12
Linda Johansson, Lisbeth Ärlestig, Heidi Kokkonen, Mikael Brink, Solbritt Rantapää-Dahlqvist
Objectives: RANK ligand (RANKL) is involved in destruction and osteoporosis in RA. In this study, the relationships between RANKL and ACPA, anti-carbamylated protein antibodies (anti-CarP), cytokines and chemokines were analysed in individuals before the onset of RA symptoms, and their associations with radiological findings at disease onset were assessed. Methods: This was a case-control study performed within the Medical Biobank of Northern Sweden that included 470 pre-symptomatic individuals [334 women and 136 men; mean (s...
September 25, 2017: Rheumatology
https://www.readbyqxmd.com/read/29026684/combined-multi-distance-frequency-domain-and-diffuse-correlation-spectroscopy-system-with-simultaneous-data-acquisition-and-real-time-analysis
#13
Stefan A Carp, Parisa Farzam, Norin Redes, Dennis M Hueber, Maria Angela Franceschini
Frequency domain near infrared spectroscopy (FD-NIRS) and diffuse correlation spectroscopy (DCS) have emerged as synergistic techniques for the non-invasive assessment of tissue health. Combining FD-NIRS oximetry with DCS measures of blood flow, the tissue oxygen metabolic rate can be quantified, a parameter more closely linked to underlying physiology and pathology than either NIRS or DCS estimates alone. Here we describe the first commercially available integrated instrument, called the "MetaOx", designed to enable simultaneous FD-NIRS and DCS measurements at rates of 10 + Hz, and offering real-time data evaluation...
September 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/29024769/identification-of-a-single-p19-gene-and-three-p40-paralogues-in-grass-carp-ctenopharyngodon-idellus-their-potential-for-the-formation-of-interleukin-23-and-inducible-expression-in-vitro-and-in-vivo
#14
Xinyan Wang, Lei Qin, Linyong Du, Di Chen, Anying Zhang, Kun Yang, Hong Zhou
Interleukin (IL-) 23, a member of IL-12 family, is a composite cytokine with the subunits of p19 and p40. Although IL-12 and IL-23 share the p40 subunit, they play vastly different roles in immune regulation. In teleost, much emphasis has been placed on the identification of IL-12, but evidence for the existence of IL-23 is still lacking. In the present study, a p19 gene and three p40 paralogues were isolated and identified from grass carp, suggesting multiple assembly of IL-23 molecules in fish species. To address this issue, the existence of different p19/p40 heterodimers were examined by Co-Immunoprecipitation (Co-IP) assay, showing that only co-expression of p19 and each p40 subunit could produce the soluble proteins corresponding to three IL-23 isoforms...
October 9, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29024067/evaluation-of-multiplex-real-time-pcr-for-identifying-dermatophytes-in-clinical-samples-a-multicentre-study
#15
Shany Sherman, Maya Goshen, Orit Treigerman, Keren Ben-Zion, Marie-Jeanne Carp, Noam Maisler, Inbal Binsky Ehrenreich, Aviva Kimchi, Sara Lifshitz, Gill Smollan, Batya Davidovici, Michael David, Emmilia Hodak, Rina Segal
BACKGROUND: The gold-standard method for dermatophyte identification involves direct microscopy and culture, which have inherent shortcomings. Only few molecular methods have been standardised for routine clinical work. OBJECTIVES: To develop and test a platform for identifying the most common dermatophytes in Israel using multiplex real-time polymerase chain reaction (RT-PCR). METHODS: Specific primers were designed for the multiplex system (LightCycler 480) according to known cultures and validated by reference isolates...
October 12, 2017: Mycoses
https://www.readbyqxmd.com/read/29022426/ignorance-is-bliss-women-s-knowledge-regarding-age-related-pregnancy-risks
#16
Michal Sheinis, Nicole Carpe, Shira Gold, Amanda Selk
Pregnancy risks rise with age and the average age of first time mothers is rising. This study aimed to assess women's actual knowledge and their perceived knowledge of pregnancy complications relating to advanced maternal age. A cross-sectional survey was administered to primiparous women measuring demographics, knowledge of age-related pregnancy risks, previous counselling and health literacy. Of the 218 women surveyed, the mean knowledge score was not significantly different for women <35 years of age compared to women ≥35 years of age (p = ...
October 12, 2017: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/29022155/some-immunological-responses-of-common-carp-cyprinus-carpio-fingerling-to-acute-extremely-low-frequency-electromagnetic-fields-50%C3%A2-hz
#17
Mahsa Mohammadi-Zadeh Khoshroo, Mehdi Shamsaie Mehrjan, Farzaneh Samiee, Mehdi Soltani, Seyed Pezhman Hosseini Shekarabi
Increasing of anthropogenic electromagnetic fields in aquatic environments has been recently become the core of attention. In this study, the effect of extremely low-frequency electromagnetic fields (50 Hz) on immune status and metabolic markers of common carp fingerling was assessed. The fish were exposed to extremely low-frequency electromagnetic fields at four intensities of 0.1, 0.5, 1, and 2 mT only once for 2 h; then, they were reared for 60 days. Results showed that the levels of aspartate aminotransferase (AST) and alanine transaminase (ALT) and alkaline phosphatase (ALP) were increased with an increase in the electromagnetic field intensity on 15 and 60 days post exposure...
October 11, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29021595/carpe-diet
#18
Elisa Viciani
No abstract text is available yet for this article.
November 2017: Nature Reviews. Microbiology
https://www.readbyqxmd.com/read/29017940/single-walled-carbon-nanotubes-as-delivery-vehicles-enhance-the-immunoprotective-effect-of-a-dna-vaccine-against-spring-viremia-of-carp-virus-in-common-carp
#19
Chen Zhang, Zhao Zhao, Ji-Wei Zha, Gao-Xue Wang, Bin Zhu
Spring viremia of carp virus (SVCV) is highly contagious and pathogenic to cyprinid fish, causing enormous economic losses in aquaculture. Efficient and economic prophylactic measure against is the most pressing desired for the common carp farming industry. In this research, single-walled carbon nanotubes (SWCNTs) as a candidate DNA vaccine carrier was administrated via bath (1, 5, 10, 20, 40 mg L(-1)) or injection (1, 4, 8, 12, 20 μg) in common carp juvenile, and the different immune treatments to induce immunoprotective effect was analyzed...
October 7, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28993970/factors-affecting-palatability-of-four-submerged-macrophytes-for-grass-carp-ctenopharyngodon-idella
#20
Jian Sun, Long Wang, Lin Ma, Fenli Min, Tao Huang, Yi Zhang, Zhenbin Wu, Feng He
Grass carp can weaken the growth and reproductive capacity of submerged macrophytes by consuming valuable tissues, but factors affecting palatability of submerged macrophytes for grass carp rarely are considered. In this study, relative consumption rate of grass carp with regard to submerged macrophytes was in the following order: Hydrilla verticillata > Vallisneria natans > Ceratophyllum demersum > Myriophyllum spicatum. Firmness of macrophytes was in the following order: M. spicatum > C. demersum > H...
October 9, 2017: Environmental Science and Pollution Research International
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