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Zhuoran Han, Jingfeng Sun, Aijun Lv, Anli Wang
This study examined the technical bias associated with different DNA extraction methods used in microbiome research. Three methods were used to extract genomic DNA from the same intestinal microbiota sample that was taken from the koi carp Cyprinus carpio var. koi, after which their microbial diversity and community structure were investigated on the basis of a 16S rDNA high-throughput sequencing analysis. Biased results were observed in relation to the number of reads, alpha diversity indexes and taxonomic composition among the three DNA extraction protocols...
April 17, 2018: MicrobiologyOpen
Tingting Liang, Yongfang Jia, Ruihua Zhang, Qiyan Du, Zhongjie Chang
Sox7, Sox17 and Sox18 are the members of the Sry-related high-mobility group box family (SoxF) of transcription factors. SoxF factors regulate endothelial cell fate as well as development and differentiation of blood cells and lymphatic vessels. There is very less information about the functions of these genes in fish. We obtained the full-length cDNA sequence of SoxF genes including Sox7, Sox17 and Sox18 in Cyprinus carpio, where Sox7 and Sox18 had two copies. The construction of a phylogenetic tree showed that these genes were homologous to the genes in other species...
March 2018: Journal of Genetics
Megumi Matsumoto, Kyosuke Araki, Sayaka Nishimura, Hideki Kuriyama, Teruyuki Nakanishi, Kazuhiro Shiozaki, Yutaka Takeuchi, Atsushi Yamamoto
Mycobacteriosis and nocardiosis in cultured fish caused by infections with acid-fast bacteria, are responsible for large economic losses globally. In this study, we suggest a novel adjuvant using glycolipids that activates host immune systems. The immune response to glycolipids stimulation was investigated using ginbuna crucian carp. Ginbuna vaccinated with FKC (formalin-killed cells) + glycolipids isolated from Mycobacterium sp., upregulated inflammatory- and Th1-related cytokines, and a DTH (delayed-type hypersensitivity) response was confirmed only in ginbuna vaccinated with FKC + glycolipids...
April 14, 2018: Developmental and Comparative Immunology
Sadegh Saffari, Saeed Keyvanshokooh, Mohammad Zakeri, Seyed Ali Johari, Hossein Pasha-Zanoosi, Mansour Torfi Mozanzadeh
An 8-week feeding trial was conducted to compare the effects of supplementing (0.7 mg kg-1 ) different dietary selenium (Se) sources including organic [selenomethionine (SeMet)], inorganic [sodium selenite (Na2 SeO3 )], and nanoparticulate Se (nano-Se) on physiological responses of common carp, Cyprinus carpio juveniles (9.7 ± 0.1 g). Basal diet without Se supplementation used as control. Fish fed nano-Se supplemented diet had the highest weight gain (97.2 ± 10.8%) and feed efficiency ratio (42...
April 16, 2018: Fish Physiology and Biochemistry
Avinash Pradhan, Madhusmita Nayak, Mrinal Samanta, Rudra Prasanna Panda, Suresh Chandra Rath, Shiba Shankar Giri, Ashis Saha
Follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh), secreted from pituitary, stimulate gonadal function by binding to their cognate receptors FSH receptor (FSHR), and LH/ choriogonadotropin receptor (LHCGR). Rohu (Labeo rohita) is a commercially important seasonal breeder freshwater fish species, but till date, the regulation of expression of gonadotropins and their receptors gene during different phases of annual reproductive cycle has not been investigated. We envisaged the critical role of these molecules during seasonal gonadal development in this carp species...
April 13, 2018: General and Comparative Endocrinology
Yueqi An, Qing Liu, Yaru Xie, Shanbai Xiong, Tao Yin, Ru Liu
BACKGROUND: Ultrasound and Ca2+ have been used separately to increase myosin properties in fish processing. However, little literature was found about myosin changes affected by Ca2+ and ultrasound in combination. The goal of this study was to investigate the effects of ultrasound-calcium combination system on aggregation and conformational changes of silver carp myosin. RESULTS: Ultrasonic facilitated the Ca2+ -induced increase of turbidity. As Ca2+ concentration increased from 0 to 100 mmol L-1 , there was an obvious increase in the turbidity, solubility and mean hydrated particle size of myosin after ultrasonic treatment compared to those without ultrasonic...
April 15, 2018: Journal of the Science of Food and Agriculture
Gyojin Choo, Hyeon-Seo Cho, Kyunghwa Park, Jae-Woo Lee, Pilje Kim, Jeong-Eun Oh
The concentrations, distributions, and bioaccumulation of nine organophosphate flame retardants (OPFRs) were investigated in both abiotic and biotic media, comprising river water, sediment, and crucian carp. The highest concentrations were observed in liver (6.22-18.1 ng/g ww), and the levels in muscle (4.23-7.75 ng/g ww) and gonad (3.08-7.70 ng/g ww) were similar. In whole blood, tris(2-butoxyethyl) phosphate (TBOEP; 31.1-256 ng/mL) accounted for 90% of the total OPFR concentration. Distributions of OPFRs differed between biotic and abiotic media, as tris(2-chloroethyl) phosphate (TCEP), tris(1-chloro-2-propyl) phosphate (TCIPP), and TBOEP were dominant in abiotic media, whereas triethyl phosphate (TEP), tri-n-butyl phosphate (TNBP), TCEP, and TBOEP dominated in crucian carp...
April 10, 2018: Environmental Pollution
Lili Suo, Weihua Huang, Qunying Zhu, Lifang Ma, Meihua Hu
BACKGROUND: A new method based on accelerated solvent extraction was developed for the extraction and determination of 11 organophosphorus flame retardants by using a high-performance liquid chromatography tandem mass spectrometry technique. RESULTS: After optimization of the extraction temperature (80 °C), the extraction solvent (n-hexane), the flush volume (40 %) and the static extraction time (4 min), all of 11 organophosphorus flame retardants illustrated good linearities (R>0...
April 13, 2018: Journal of the Science of Food and Agriculture
Parisa Farzam, Erin M Buckley, Pei-Yi Lin, Katherine Hagan, P Ellen Grant, Terrie Eleanor Inder, Stefan A Carp, Maria Angela Franceschini
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.
April 12, 2018: Scientific Reports
J J Su, X Y Shang, Q Y Wan, J G Su
Eleven single nucleotide polymorphisms (SNP) in Ctenopharyngodon idella toll-like receptor 7 (citlr7) gene, containing two in the 5'-flanking region, three within the single intron and six distributed in the coding sequence (CDS), were identified. A case-control study of 73 susceptible individuals and 67 resistant individuals was conducted to test the SNPs-based susceptibility-resistance association and mRNA expression of citlr7 to grass carp reovirus (GCRV), showing that both 820 A/G and 1726 A/G were significantly correlative sites in genotype (P < 0·05)...
April 11, 2018: Journal of Fish Biology
Yanfeng Lv, Xiaoxiang Zhao, Lian Yang, Xian Zhang, Yujie Bai
The degradation of polycyclic aromatic hydrocarbons (PAHs) can generate AhR-binding compounds, exhibiting genotoxicity and carcinogenicity. In this investigation, aryl hydrocarbon receptor (AhR) from carp and anthracene (Ant) were coupled as antigen to establish an indirect competition ELISA (ic-ELISA) with an AhR-Ant antibody. A standard curve was determined for the ic-ELISA concerning detection range and limit. Also, the specificity, stability and the recovery of the ic-ELISA were checked. Results indicate that the ratio of antibody to antigen titer is 1:64000...
2018: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
Lv Xiong, Libo He, Lifei Luo, Yongming Li, Lanjie Liao, Rong Huang, Zuoyan Zhu, Yaping Wang
Grass carp reovirus (GCRV) causes huge economic loss to the grass carp cultivation industry but the mechanism remains largely unknown. In this study, we investigated the global and complement gene-specific DNA methylation in grass carp after GCRV infection aimed to uncover the mechanism underlying GCRV infection. The global DNA methylation level was increased after GCRV infection. Expression levels of enzymes involved in DNA methylation including DNA methyltransferase (DNMT), ten-eleven translocation proteins (TETs), and glycine N -methyltransferase (GNMT) were significantly altered after GCRV infection...
April 7, 2018: International Journal of Molecular Sciences
Katarzyna Lugowska
To determine the effects of Roundup, a commercial formulation of glyphosate, gametes, and embryos of common carp (Cyprinus carpio L) was exposed to wide range of herbicide concentrations (0.0, 0.1, 0.5, 2.0, 5.0, 10.0, 20.0, and 50.0 mg/l). The obtained results showed different effects of Roundup on common carp gametes. Herbicide reduced swelling of eggs (but the effect was not concentration-related), while sperm showed low sensitivity to Roundup (time of spermatozoa motility was reduced in a significant way only at 20 mg/l, and at remaining concentrations, only a slight tendency was observed)...
April 7, 2018: Fish Physiology and Biochemistry
Jialu Sheng, Fei Yu, Dubo Chen, Hao Wang, Liqun Lu
Fibulin-4 is not only involved in connective tissue development and elastic fiber formation, but also plays critical neoplastic roles in tumor growth by activating Wnt/β-Catenin signaling in human. Recently, Fibulin-4 was shown to associate with grass carp reovirus (GCRV) outer capsid proteins and might relate to viral hemorrhagic disease in grass carp Ctenopharyngodon idella. Here, we monitored the expression pattern of Fibulin-4 during the infection course of GCRV at both translational and transcriptional levels, and found that Fibulin-4 was significantly suppressed upon the viral challenge in grass cap GCO cells...
April 5, 2018: Fish & Shellfish Immunology
Wenjue Zhong, Yanfeng Zhang, Zihao Wu, Rongyan Yang, Xinyue Chen, Jing Yang, Lingyan Zhu
The distribution and potential health risks of eight heavy metals (Copper (Cu), Chromium (Cr), Zinc (Zn), Lead (Pb), Arsenic (As), Cadmium (Cd), Manganese (Mn), Nickel (Ni)) in 16 freshwater systems from central and eastern North China, were investigated. The fish were divided as wild fish, which grew naturally without artificially feeding, and farmed fish. The total concentrations of the eight heavy metals ranged from 82.9 to 226 μg/L in the surface water samples and 3.32-27.6 mg/kg dw in the fish samples...
April 5, 2018: Ecotoxicology and Environmental Safety
Junya Wang, Nianzhi Zhang, Zhenbao Wang, Wu Yanan, Lijie Zhang, Chun Xia
The CD8αα homodimer structures of endotherms demonstrate that despite distinct diversity at the amino acid sequence level, a few conserved key amino acids ensure common structural features. The structure of CD8αα in ancient ectotherms, such as lower bony fish, remains unclear. In this study, the high-resolution structure of the grass carp (Ctenopharyngodon idella) CD8αα (Ctid-CD8αα) homodimer was determined using the single-wavelength anomalous diffraction (SAD) method. The structure of Ctid-CD8αα shows distinct differences from the known CD8αα structures of endotherms, including a distinct topological structure with shorter back β sheets...
April 4, 2018: Molecular Immunology
Lifei Luo, Rong Huang, Aidi Zhang, Cheng Yang, Liangming Chen, Denghui Zhu, Yongming Li, Libo He, Lanjie Liao, Zuoyan Zhu, Yaping Wang
Transgenic Yellow River carp is characterized by rapid growth rate and high feed-conversion efficiency and exhibits a great application prospect. However, there is still a significant separation of growth traits in the transgenic Yellow River carp family; as such, growth-related genotypes must be screened for molecular marker-assisted selection. In this study, 23 growth-related candidate genes containing 48 SNP markers were screened through bulked segregant analysis (BSA) among transgenic Yellow River carp family members showing significant separation of growth traits...
April 5, 2018: Functional & Integrative Genomics
Yueqi An, Juan You, Shanbai Xiong, Tao Yin
To enhance the application of transglutaminase (TGase) in processing and preserving of frozen surimi, the mechanism that enhances surimi gelation induced by TGase after short-term freezing was investigated. Gel properties and cross-linking extent of surimi gels increased when surimi was frozen at -18 °C for 5-7 days. However when storage time exceeded 10 days, textural properties and water-holding capacity decreased significantly. Moreover, the difference of breaking force and hardness between surimi gels with and without TGase reached the maximum after 3-5 days of frozen storage...
August 15, 2018: Food Chemistry
Anna Rymuszka, Anna Sieroslawska
Nodularin, one of the toxins produced by cyanobacteria commonly occurring in brackish waters, is a cyclic pentapeptide, that may exert adverse effects on animal and human health. The aim of the present study was to evaluate the cytotoxicity of nodularin on different fish cellular models. The leukocytes isolated from carp head kidney and common carp (Cyprinus carpio L.) leukocyte cell line (CLC) were incubated with the cyanotoxin at the concentrations of 0.001, 0.01, 0.05 or 0.1 μg/mL. Treatment of cells with the higher concentrations of the toxin resulted in a dramatic decrease in cell viability...
April 2, 2018: Toxicon: Official Journal of the International Society on Toxinology
Yali Shi, Robin Vestergren, Therese Haugdahl Nost, Zhen Zhou, Yaqi Cai
Understanding the bioaccumulation mechanisms of per- and polyfluoroalkyl substances (PFASs) across different chain-lengths, isomers and functional groups represents a monumental scientific challenge with implications for chemical regulation. Here, we investigate how the differential tissue distribution and bioaccumulation behavior of 25 PFASs in crucian carp from two field sites impacted by point sources can provide information about the processes governing uptake, distribution and elimination of PFASs. Median tissue/blood ratios (TBRs) were consistently <1 for all PFASs and tissues except bile which displayed a distinct distribution pattern and enrichment of several perfluoroalkyl sulfonic acids...
April 3, 2018: Environmental Science & Technology
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