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https://www.readbyqxmd.com/read/28330330/a-combined-chloroplast-atpb-rbcl-and-trnl-f-phylogeny-unveils-the-ancestry-of-balsams-impatiens-spp-in-the-western-ghats-of-india
#1
P P Shajitha, N R Dhanesh, P J Ebin, Joseph Laly, Devassy Aneesha, John Reshma, Jomy Augustine, Mathew Linu
Only a few Impatiens spp. from South India (one of the five centers of diversity for Impatiens species) were included in the published datum of molecular phylogeny of the family Balsaminaceae. The present investigation is a novel attempt to reveal the phylogenetic association of Impatiens species of South India, by placing them in the global phylogeny of Impatiens based on a combined analysis of two chloroplast genes. Thirty species of genus Impatiens were collected from different locations of South India. Total genomic DNA was extracted from fresh plant leaf, and polymerase chain reaction was carried out using atpB-rbcL and trnL-F intergenic spacer-specific forward and reverse primers...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330302/production-of-bioherbicide-by-phoma-sp-in-a-stirred-tank-bioreactor
#2
Thiarles Brun, Jéssica E Rabuske, Izelmar Todero, Thiago C Almeida, Jair J D Junior, Gustavo Ariotti, Tássia Confortin, Jonas A Arnemann, Raquel C Kuhn, Jerson V C Guedes, Marcio A Mazutti
The objective of this work was to produce an herbicide by submerged fermentation in a stirred-tank bioreactor and to assess the potential herbicidal in pre-emergence, post-emergence, and in a detached leaves of Cucumis sativus var species. wisconsin (cucumber) and Sorghum bicolor (sorghum) species. Fermentations were carried out in a stirred-tank bioreactor with useful volume of 3L. Stirring rate (40, 50, and 60 rpm) and aeration (1, 2 and 3 vvm) were the variables studied for bioherbicide production. Fermented broth was fractioned with different solvents to identify the molecules produced by the fungus in a multi-dimensional gas chromatograph system...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330298/biodegradation-of-used-engine-oil-by-novel-strains-of-ochrobactrum-anthropi-hm-1-and-citrobacter-freundii-hm-2-isolated-from-oil-contaminated-soil
#3
Haytham M M Ibrahim
Used engine oil (UEO) constitutes a serious environmental problem due to the difficulty of disposal off or reuse. Ten bacterial strains with biodegradation potential were isolated from UEO-contaminated soil sample using enrichment technique. Two strains which exhibited the highest degradation %, 51 ± 1.2 and 48 ± 1.5, respectively, were selected. Based on the morphological, biochemical characteristics and 16S rRNA sequence analysis, they were identified as Ochrobactrum anthropi HM-1 (accession no: KR360745) and Citrobacter freundii HM-2 (accession no: KR360746)...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330296/application-of-pectinases-for-recovery-of-grape-seeds-phenolics
#4
Petra Štambuk, Dora Tomašković, Ivana Tomaz, Luna Maslov, Domagoj Stupić, Jasminka Karoglan Kontić
Grape marc seeds contain large amounts of different polyphenolic compounds so they can be used for the recovery of these classes of compounds. A new green extraction method for the recovery of phenols from the grape seeds was developed. To provide a high quality extract sourced from natural product by using enzymes as alternative solvents and spending less energy, it is impeccable to call this method "green". Furthermore, this method was optimized by finding out which conditions provide the best possible results and achieving the maximum recovery of polyphenols from grape seeds...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330294/immuno-hybrid-algorithm-a-novel-hybrid-approach-for-grn-reconstruction
#5
A S Jereesh, V K Govindan
Bio-inspired algorithms are widely used to optimize the model parameters of GRN. In this paper, focus is given to develop improvised versions of bio-inspired algorithm for the specific problem of reconstruction of gene regulatory network. The approach is applied to the data set that was developed by the DNA microarray technology through biological experiments in the lab. This paper introduced a novel hybrid method, which combines the clonal selection algorithm and BFGS Quasi-Newton algorithm. The proposed approach implemented for real world E...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330291/optimization-of-culture-conditions-by-response-surface-methodology-and-unstructured-kinetic-modeling-for-bioactive-metabolite-production-by-nocardiopsis-litoralis-vsm-8
#6
Ushakiranmayi Managamuri, Muvva Vijayalakshmi, Sudhakar Poda, V S Rama Krishna Ganduri, R Satish Babu
Response surface methodology-based central composite design on five variables incubation time, pH, temperature, sucrose concentration, and soya peptone concentration was employed for optimization of the production of bioactive compounds by Nocardiopsis litoralis strain VSM 8. The main quadratic effects and interactions of the five variables on the production of bioactive metabolites were investigated. A second-order polynomial model produced a satisfied fit for experimental data with regard to the production of the bioactive metabolites...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330288/biofabrication-and-spectral-characterization-of-silver-nanoparticles-and-their-cytotoxic-studies-on-human-cd34-ve-stem-cells
#7
Venkata S Kotakadi, Susmila Aparna Gaddam, Sucharitha K Venkata, P V G K Sarma, D V R Sai Gopal
Biosynthesis of plant-mediated silver nanoparticles is gaining significant importance due to environmentally safe 'green method' and it is an efficient alternative method. In the present study, silver nanoparticles were synthesized by using root extract of Glycyrrhiza glabra an important medicinal plant. The AgNPs are characterized by spectral analysis; the surface plasmon resonance (SPR) peak of AgNPs showed maximum absorption at 445 nm. Fourier-transform infrared spectroscopy (FT-IR) data show that the O-H hydroxyl groups, carboxylic acids, ester and ether groups and C-O stretching of alcohols have been utilized in the formation of AgNPs...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330285/screening-production-optimization-and-characterization-of-%C3%AE-glucosidase-using-microbes-from-shellfish-waste
#8
Samhita Mahapatra, A S Vickram, T B Sridharan, R Parameswari, M Ramesh Pathy
An extracellular β-glucosidase was isolated from Proteus mirabilis VIT117 found to be growing on prawn shells. The enzyme production was found to be enhanced (14.58 U/ml) when the culture was maintained at pH 9 and provided with sorbitol as carbon source, yeast extract as nitrogen source and incubated at 37 °C for approximately 72 h. Statistical methods like Plackett-Burman and RSM were also applied here to study the effects of different combinations of growth parameters for the bacteria, where the most significant parameters were found to be inoculum size, pH, yeast extract, incubation time and sorbitol...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330278/effects-of-the-culture-media-optimization-on-pectinase-production-by-enterobacter-sp-pstb-1
#9
M Purna Chandra Reddy, K V Saritha
In the present study, media composition for high production of pectinase by Enterobacter sp. PSTB-1 in submerged fermentation was optimized using response surface methodology (RSM). Mango fruit processing industrial waste (MIW) was used as substrate (carbon source) as it contains high amount of pectin. Enterobacter sp. PSTB-1 used in present study has given pectin clear zone (PCZ) of 34 mm is higher than other isolates. The experimental results made by statistical design for high pectinase production revealed that the suitable media components: NaNO3 2...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330269/identification-of-the-traditional-and-non-traditional-sulfate-reducing-bacteria-associated-with-corroded-ship-hull
#10
Kiana Alasvand Zarasvand, V Ravishankar Rai
Pitting corrosion due to microbial activity is the most severe type of corrosion that occurs in ship hull. Since biogenic sulfide produced by sulfate-reducing bacteria (SRB) is involved in the acceleration of pitting corrosion of marine vessels, so it is important to collect information about SRB community involved in maritime vessel failure. We investigated the SRB community on corroded hull portion of the ship. With the use of common cultural method and 16S rDNA sequencing, ten bacteria with sulfate reduction ability were isolated and identified...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330261/purification-and-characterization-of-laccase-from-marasmius-species-bbkav79-and-effective-decolorization-of-selected-textile-dyes
#11
A B Vantamuri, B B Kaliwal
A novel laccase-producing white-rot fungus, Marasmius sp. BBKAV79 (Genbank Accession Number-KP455496, KP455497), was isolated and subjected to purification, characterization and dye decolorization study. The purified enzyme was obtained with a specific activity of 0.226 U mg(-1) protein and a final yield of 13.5 %. The enzyme was found to be a monomeric protein with a molecular mass of ~75 kDa as estimated by non-denaturing polyacrylamide gel electrophoresis (PAGE) and further confirmed with zymogram analysis...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330260/impact-of-forest-vegetation-on-soil-characteristics-a-correlation-between-soil-biological-and-physico-chemical-properties
#12
L R Chandra, S Gupta, V Pande, N Singh
Temperate and dry deciduous forest covers major portion of terrestrial ecosystem in India. The two forest types with different dominant tree species differ in litter quality and root exudates, thereby exerting species-specific impact on soil properties and microbial activity. This study aims to examine the influence of forest type or dominant tree species on soil physico-chemical properties and its relationship with microbial characters in temperate and dry deciduous forest types. We assessed soil physico-chemical properties among five different sites located within the selected forest stand covered by different dominant species...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330256/immobilized-lipase-catalyzing-glucose-stearate-synthesis-and-their-surfactant-properties-analysis
#13
A Maria Sebatini, Manisha Jain, P Radha, S Kiruthika, Krishnamurthi Tamilarasan
Sugar fatty acid esters are practical importance and have a variety of applications that include surfactants and as an emulsifying agent. In this study, we report glucose stearate synthesis using lipase-Fe3O4 nanoparticles catalyst. The influence of various reaction factors, such as silica gel concentration, molar ratio of sugar/acid, reaction temperature and speed of agitation on esterification by immobilized enzyme was analyzed. The glucose stearate esterification degree of 87.2 % was obtained under the optimized condition: 1:2 molar ratio of glucose/stearic acid, 2 % (w/v) of silica gel at 120 rpm and 40 °C...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330252/organic-solvent-tolerant-metallo-protease-of-novel-isolate-serratia-marcescens-ppb-26-production-and-characterization
#14
Shikha Thakur, Nirmal Kant Sharma, Neerja Thakur, Savitri, Tek Chand Bhalla
Proteases are a class of enzymes that catalyze hydrolysis of peptide bonds of proteins. In this study, 221 proteolytic bacterial isolates were obtained by enrichment culture method from soils of various regions of Himachal Pradesh, India. From these a hyper producer of protease was screened and identified by morphological and physiological testing and by 16S rDNA sequence as Serratia marcescens PPB-26. Statistical optimization of physiochemical parameters enhanced the protease production by 75 %. Protease of S...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330249/emulsifying-activity-of-a-biosurfactant-produced-by-a-marine-bacterium
#15
K Abraham Peele, V Ravi Teja Ch, Vidya P Kodali
Biosurfactants produced by biofilm-forming bacteria have great applications in biotechnology, pharmaceutical, food engineering, bioremediation, and biohydrometallurgy industries. This study aimed to find out the bacteria that produce novel exopolymers (EPSs) which can find potential role in oil biodegradation. A screening procedure was performed to detect EPS-producing bacteria. The EPS producing isolate was identified as Acinetobacter species by 16S rDNA analysis. The polymer produced by the isolate has shown significant emulsification and surfactant activities, and the activities were compared to some of the commercial emulsifiers...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330241/purification-characterization-gene-cloning-and-expression-of-gh-10-xylanase-penicillium-citrinum-isolate-hzn13
#16
Zabin K Bagewadi, Sikandar I Mulla, Harichandra Z Ninnekar
An extracellular thermostable xylanase (Xyl-IIb) produced by Penicillium citrinum isolate HZN13 was purified to homogeneity using DEAE-Sepharose, Sephadex G-100 and Bio-Gel P-60 chromatography with specific activity of 6272.7 U/mg and 19.6-fold purification. The purification revealed the occurrence of multiple forms of xylanases (Xyl-I, Xyl-IIa, Xyl-IIb and Xyl-III). The molecular mass of highly purified Xyl-IIb was ~31 kDa with SDS-PAGE. The enzyme was cellulase-free, thermostable (55-75 °C) and acidophilic (3...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330240/a-novel-cyclodextrin-glucanotransferase-from-an-alkaliphile-microbacterium-terrae-knr-9-purification-and-properties
#17
Kiransinh N Rajput, Kamlesh C Patel, Ujjval B Trivedi
Cyclodextrin glucanotransferase (CGTase, EC. 2.1.1.19) produced using new alkaliphile Microbacterium terrae KNR 9 has been purified to homogeneity in a single step by the starch adsorption method. The specific activity of the purified CGTase was 45 U/mg compared to crude 0.9 U/mg. This resulted in a 50-fold purification of the enzyme with 33 % yield. The molecular weight of the purified enzyme was found to be 27.72 kDa as determined by SDS-PAGE. Non-denaturing gel electrophoresis and activity staining confirmed the presence of CGTase in crude and the ammonium sulfate precipitate fraction...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330236/xylanase-production-from-penicillium-citrinum-isolate-hzn13-using-response-surface-methodology-and-characterization-of-immobilized-xylanase-on-glutaraldehyde-activated-calcium-alginate-beads
#18
Zabin K Bagewadi, Sikandar I Mulla, Yogesh Shouche, Harichandra Z Ninnekar
The present study reports the production of high-level cellulase-free xylanase from Penicillium citrinum isolate HZN13. The variability in xylanase titers was assessed under both solid-state (SSF) and submerged (SmF) fermentation. SSF was initially optimized with different agro-waste residues, among them sweet sorghum bagasse was found to be the best substrate that favored maximum xylanase production (9643 U/g). Plackett-Burman and response surface methodology employing central composite design were used to optimize the process parameters for the production of xylanase under SSF...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330234/optimization-of-cellulase-production-by-penicillium-sp
#19
H N Prasanna, G Ramanjaneyulu, B Rajasekhar Reddy
The production of cellulolytic enzymes (β-exoglucanase, β-endoglucanase and β-glucosidase) by Penicillium sp. on three different media in liquid shake culture conditions was compared. The organism exhibited relatively highest activity of endoglucanase among three enzymes measured at 7-day interval during the course of its growth on Czapek-Dox medium supplemented with 0.5 % (w/v) cellulose. Cellulose at 0.5 %, lactose at 0.5 %, sawdust at 0.5 %, yeast extract at 0.2 % as a nitrogen source, pH 5.0 and 30 °C temperature were found to be optimal for growth and cellulase production by Penicillium sp...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330233/improvement-of-shelf-life-of-soymilk-using-immobilized-protease-of-oerskovia-xanthineolytica-ncim-2839
#20
A K Sahoo, V S Gaikwad, R C Ranveer, P B Dandge, S R Waghmare
Protease enzyme has lot of commercial applications, so the cost-effective production of protease using sunflower oil seed waste was carried out from Oerskovia xanthineolyitca NCIM 2839. The maximum protease production was after 24 h of incubation with 2.5 % oil seed waste concentration. O. xanthineolytica was found to produce two proteases-P1 and P2. The proteases were purified using 60 % cold acetone precipitation and DEAE-cellulose ion exchange chromatography. SDS-PAGE revealed molecular weight of P1 and P2 was 36 and 24 kDa, respectively...
December 2016: 3 Biotech
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