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Isolation of endothelial progenitor cells

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https://www.readbyqxmd.com/read/29117787/reprogramming-of-adult-peripheral-blood-cells-into-human-induced-pluripotent-stem-cells-as-a-safe-and-accessible-source-of-endothelial-cells
#1
Pavel Simara, Lenka Tesarova, Daniela Rehakova, Simon Farkas, Barbara Salingova, Katerina Kutalkova, Eva Vavreckova, Pavel Matula, Petr Matula, Lenka Veverkova, Irena Koutna
New approaches in regenerative medicine and vasculogenesis have generated a demand for sufficient numbers of human endothelial cells (ECs). ECs and their progenitors (EPCs) reside on the interior surface of blood and lymphatic vessels or circulate in peripheral blood; however, their numbers are limited and they are difficult to expand after isolation. Recent advances in human induced pluripotent stem cell (hiPSC) research have opened possible avenues to generate unlimited numbers of ECs from easily accessible cell sources, such as the peripheral blood...
November 8, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/29113460/impact-of-age-on-human-adipose-stem-cells-for-bone-tissue-engineering
#2
Denis Dufrane
Bone nonunion is a pathological condition in which all bone healing processes have stopped, resulting in abnormal mobility between 2 bone segments. The incidence of bone-related injuries will increase in an aging population, leading to such injuries reaching epidemic proportions. Tissue engineering and cell therapy using mesenchymal stem cells (MSCs) have raised the possibility of implanting living tissue for bone reconstruction. Bone marrow was first proposed as the source of stem cells for bone regeneration...
September 2017: Cell Transplantation
https://www.readbyqxmd.com/read/29113197/acidic-stress-induces-apoptosis-and-inhibits-angiogenesis-in-human-bone-marrow-derived-endothelial-progenitor-cells
#3
Shuai Huang, Peiheng He, Dongliang Xu, Jinglei Li, Xinsheng Peng, Yubo Tang
Bone marrow-derived endothelial progenitor cells (BM-EPCs) are exposed to acidotic environments in a variety of physiological and pathological conditions, including in tumors. However, the effect of acidosis on the function of BM-EPCs is still not fully understood. In the present study, BM-EPCs were isolated and cultured at an extracellular pH (pHe) of 6.5 or pHe 7.4 in vitro prior to various experiments being performed. Cellular proliferation, migration and tube formation ability were detected by DNA content quantification, Transwell assay and Matrigel-based angiogenesis assay...
November 2017: Oncology Letters
https://www.readbyqxmd.com/read/29101610/endothelial-microparticles-are-associated-to-pathogenesis-of-idiopathic-pulmonary-fibrosis
#4
Nour C Bacha, Adeline Blandinieres, Elisa Rossi, Nicolas Gendron, Nathalie Nevo, Séverine Lecourt, Coralie L Guerin, Jean Marie Renard, Pascale Gaussem, Eduardo Angles-Cano, Chantal M Boulanger, Dominique Israel-Biet, David M Smadja
Idiopathic pulmonary fibrosis (IPF) is a devastating disease characterized by obliteration of alveolar architecture, resulting in declining lung function and ultimately death. Pathogenic mechanisms remain unclear but involve a concomitant accumulation of scar tissue together with myofibroblasts activation. Microparticles (MPs) have been investigated in several human lung diseases as possible pathogenic elements, prognosis markers and therapeutic targets. We postulated that levels and cellular origins of circulating MPs might serve as biomarkers in IPF patients and/or as active players of fibrogenesis...
November 3, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/29096622/tissue-engineered-smooth-muscle-cell-and-endothelial-progenitor-cell-bi-level-cell-sheets-prevent-progression-of-cardiac-dysfunction-microvascular-dysfunction-and-interstitial-fibrosis-in-a-rodent-model-of-type-1-diabetes-induced-cardiomyopathy
#5
Masashi Kawamura, Michael J Paulsen, Andrew B Goldstone, Yasuhiro Shudo, Hanjay Wang, Amanda N Steele, Lyndsay M Stapleton, Bryan B Edwards, Anahita Eskandari, Vi N Truong, Kevin J Jaatinen, Arnar B Ingason, Shigeru Miyagawa, Yoshiki Sawa, Y Joseph Woo
BACKGROUND: Diabetes mellitus is a risk factor for coronary artery disease and diabetic cardiomyopathy, and adversely impacts outcomes following coronary artery bypass grafting. Current treatments focus on macro-revascularization and neglect the microvascular disease typical of diabetes mellitus-induced cardiomyopathy (DMCM). We hypothesized that engineered smooth muscle cell (SMC)-endothelial progenitor cell (EPC) bi-level cell sheets could improve ventricular dysfunction in DMCM. METHODS: Primary mesenchymal stem cells (MSCs) and EPCs were isolated from the bone marrow of Wistar rats, and MSCs were differentiated into SMCs by culturing on a fibronectin-coated dish...
November 2, 2017: Cardiovascular Diabetology
https://www.readbyqxmd.com/read/29080864/endothelial-progenitor-cell-biology-and-vascular-recovery-following-transradial-cardiac-catheterization
#6
Andrew Mitchell, Takeshi Fujisawa, Nicholas L Mills, Mairi Brittan, David E Newby, Nicholas L M Cruden
BACKGROUND: Transradial catheterization is associated with radial artery injury and vasomotor dysfunction and represents an accessible model of acute vascular injury in humans. We characterized vascular injury and functional recovery to understand the role of circulating endothelial progenitor cells in vascular repair. METHODS AND RESULTS: In 50 patients (aged 64±10 years, 70% male) undergoing transradial cardiac catheterization, radial artery injury was assessed by optical coherence tomography and examination of explanted vascular sheaths...
October 28, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/29067104/isolation-and-characterization-of-human-umbilical-cord-derived-endothelial-colony-forming-cells
#7
Hao Zhang, Yanling Tao, Saisai Ren, Haihui Liu, Hui Zhou, Jiangwei Hu, Yongyong Tang, Bin Zhang, Hu Chen
Endothelial colony-forming cells (ECFCs) are a population of endothelial progenitor cells (EPCs) that display robust proliferative potential and vessel-forming capability. Previous studies have demonstrated that a limited number of ECFCs may be obtained from adult bone marrow, peripheral blood and umbilical cord (UC) blood. The present study describes an effective method for isolating ECFCs from human UC. The ECFCs derived from human UC displayed the full properties of EPCs. Analysis of the growth kinetics, cell cycle and colony-forming ability of the isolated human UC-ECFCs indicated that the cells demonstrated properties of stem cells, including relative stability and rapid proliferation in vitro...
November 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29048335/bone-marrow-derived-stem-cell-populations-are-differentially-regulated-by-thyroid-or-and-ovarian-hormone-loss
#8
Bassam F Mogharbel, Eltyeb Abdelwahid, Ana C Irioda, Julio C Francisco, Rossana B Simeoni, Daiany de Souza, Carolina M C O de Souza, Míriam P Beltrame, Reginaldo J Ferreira, Luiz C Guarita-Souza, Katherine A T de Carvalho
Bone marrow-derived stem cells (BMDSCs) play an essential role in organ repair and regeneration. The molecular mechanisms by which hormones control BMDSCs proliferation and differentiation are unclear. Our aim in this study was to investigate how a lack of ovarian or/and thyroid hormones affects stem cell number in bone marrow lineage. To examine the effect of thyroid or/and ovarian hormones on the proliferative activity of BMDSCs, we removed the thyroid or/and the ovaries of adult female rats. An absence of ovarian and thyroid hormones was confirmed by Pap staining and Thyroid Stimulating Hormone (TSH) measurement, respectively...
October 19, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29047405/cortisol-inhibits-mtor-signaling-in-avascular-necrosis-of-the-femoral-head
#9
Yun Liao, Rui Su, Ping Zhang, Bo Yuan, Ling Li
BACKGROUND: ANFH is a major health problem, to which long lasting and definitive treatments are lacking. The aim of this study is to study RNA alterations attributed to cortisol-induced ANFH. METHODS: Rat models were stratified into three groups: in vitro group (n = 20) for molecular biological assays, control group (n = 3), and ANFH group induced using lipopolysaccharide and dexamethasone (n = 3). Bone marrow-derived endothelial progenitor cells (BM-EPCs) were extracted from the rats...
October 18, 2017: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/29038672/safety-analysis-of-autologous-stem-cell-therapy-in-a-variety-of-degenerative-diseases-and-injuries-using-the-stromal-vascular-fraction
#10
Kristin Comella, Michelle Parlo, Rosemary Daly, Vincent Depasquale, Eric Edgerton, Patrick Mallory, Roy Schmidt, Walter P Drake
BACKGROUND: Stem cells from adipose tissue offer a novel therapy for patients with damaged tissue. Stromal vascular fraction (SVF) injected into patients may reduce inflammation, promote healing, and repair damaged/scarred tissue. SVF can be isolated from fat (adipose) tissue in an outpatient procedure. The SVF population includes mesenchymal stem cells (MSCs), pericytes, endothelial/progenitor cells, fibroblasts and growth factors where the adipocyte (fat cell) population has been removed...
November 2017: Journal of Clinical Medicine Research
https://www.readbyqxmd.com/read/29029631/neurotrophic-support-by-traumatized-muscle-derived-multipotent-progenitor-cells-role-of-endothelial-cells-and-vascular-endothelial-growth-factor-a
#11
Heidi R H Zupanc, Peter G Alexander, Rocky S Tuan
BACKGROUND: Adult mesenchymal stem cells (MSCs) have been shown to increase nerve regeneration in animal models of nerve injury. Traumatized muscle-derived multipotent progenitor cells (MPCs) share important characteristics with MSCs and are isolated from severely damaged muscle tissue following surgical debridement. Previous investigations have shown that MPCs may be induced to increase production of several neurotrophic factors, suggesting the possible utility of autologous MPCs in peripheral nerve regeneration following injury...
October 13, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29022504/endothelial-and-circulating-progenitor-cells-in-hematological-diseases-and-allogeneic-hematopoietic-stem-cell-transplantation
#12
Annalisa Ruggeri, Annalisa Paviglianiti, Fernanda Volt, Chantal Kenzey, Hanadi Rafii, Vanderson Rocha, Eliane Gluckman
Circulating endothelial cells (CECs), originated form endothelial progenitors (EPCs) are mature cells which are not associated with vessel walls, and that are detached from the endothelium. Normally, they are present in insignificant amounts in the peripheral blood of healthy individuals. On the other hand, elevated CECs and EPCs levels have been reported in the peripheral blood of patients with different types of cancers and some other diseases. Consequently, CECs and EPCs represent a potential biomarker in several clinical conditions involving endothelial turnover and remodeling, such as hematological diseases...
October 12, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/29018244/bioengineering-of-functional-human-induced-pluripotent-stem-cell-derived-intestinal-grafts
#13
Kentaro Kitano, Dana M Schwartz, Haiyang Zhou, Sarah E Gilpin, Gregory R Wojtkiewicz, Xi Ren, Cesar A Sommer, Amalia V Capilla, Douglas J Mathisen, Allan M Goldstein, Gustavo Mostoslavsky, Harald C Ott
Patients with short bowel syndrome lack sufficient functional intestine to sustain themselves with enteral intake alone. Transplantable vascularized bioengineered intestine could restore nutrient absorption. Here we report the engineering of humanized intestinal grafts by repopulating decellularized rat intestinal matrix with human induced pluripotent stem cell-derived intestinal epithelium and human endothelium. After 28 days of in vitro culture, hiPSC-derived progenitor cells differentiate into a monolayer of polarized intestinal epithelium...
October 10, 2017: Nature Communications
https://www.readbyqxmd.com/read/29016733/endothelial-to-haematopoietic-transition-contributes-to-pulmonary-arterial-hypertension
#14
Olin D Liang, Eui-Young So, Pamela C Egan, Laura R Goldberg, Jason M Aliotta, Keith Q Wu, Patrycja M Dubielecka, Corey E Ventetuolo, Anthony M Reginato, Peter J Quesenberry, James R Klinger
Aims: The pathogenic mechanisms of pulmonary arterial hypertension (PAH) remain unclear, but involve dysfunctional endothelial cells (ECs), dysregulated immunity and inflammation in the lung. We hypothesize that a developmental process called endothelial to haematopoietic transition (EHT) contributes to the pathogenesis of pulmonary hypertension (PH). We sought to determine the role of EHT in mouse models of PH, to characterize specific cell types involved in this process, and to identify potential therapeutic targets to prevent disease progression...
November 1, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28994769/isolation-of-endothelial-progenitor-cells-from-human-umbilical-cord-blood
#15
Prashanth Ravishankar, M Alejandra Zeballos, Kartik Balachandran
The existence of endothelial progenitor cells (EPCs) in peripheral blood and its involvement in vasculogenesis was first reported by Ashara and colleagues(1). Later, others documented the existence of similar types of EPCs originating from bone marrow(2)(,)(3). More recently, Yoder and Ingram showed that EPCs derived from umbilical cord blood had a higher proliferative potential compared to ones isolated from adult peripheral blood(4)(,)(5)(,)(6). Apart from being involved in postnatal vasculogenesis, EPCs have also shown promise as a cell source for creating tissue-engineered vascular and heart valve constructs(7)(,)(8)...
September 14, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28971999/identification-of-mir-124-as-a-major-regulator-of-enhanced-endothelial-cell-glycolysis-in-pulmonary-arterial-hypertension-via-ptbp1-and-pkm2
#16
Paola Caruso, Benjamin J Dunmore, Kenny Schlosser, Sandra Schoors, Claudia C Dos Santos, Carol Perez-Iratxeta, Jessie R Lavoie, Hui Zhang, Lu Long, Amanda R Flockton, Maria G Frid, Paul D Upton, Angelo D'Alessandro, Charaka Hadinnapola, Fedir N Kiskin, Mohamad Taha, Liam A Hurst, Mark L Ormiston, Akiko Hata, Kurt R Stenmark, Peter Carmeliet, Duncan J Stewart, Nicholas W Morrell
Background -Pulmonary arterial hypertension (PAH) is characterized by abnormal growth and enhanced glycolysis of pulmonary artery endothelial cells (PAECs). However, the mechanisms underlying alterations in energy production have not been identified. Methods -Here, we examined the miRNA and proteomic profiles of blood outgrowth endothelial cells (BOECs) from patients with heritable PAH (HPAH) due to mutations in the bone morphogenetic protein receptor type 2 (BMPR2) gene and patients with idiopathic PAH (IPAH) to determine mechanisms underlying abnormal endothelial glycolysis...
September 26, 2017: Circulation
https://www.readbyqxmd.com/read/28960647/long-term-fate-of-human-fetal-liver-progenitor-cells-transplanted-in-injured-mouse-livers
#17
Antony Irudayaswamy, Mark Muthiah, Lei Zhou, Hau Hung, Nur Halisah Bte Jumat, Jamil Haque, Narcissus Teoh, Geoffrey Farrell, Kimberly J Riehle, Jaymie Siqi Lin, Lin Lin Su, Jerry Ky Chan, Mahesh Choolani, Peng Cheang Wong, Aileen Wee, Seng Gee Lim, Jean Campbell, Nelson Fausto, Yock Young Dan
Liver progenitor cells have the potential to repair and regenerate a diseased liver. The success of any translational efforts, however, hinges on thorough understanding of the fate of these cells after transplant, especially in terms of long-term safety and efficacy. Here, we report transplantation of a liver progenitor population isolated from human fetal livers into immune-permissive mice with follow-up up to 36 weeks after transplant. We found that human progenitor cells engraft and differentiate into functional human hepatocytes in the mouse, producing albumin, alpha-1-antitrypsin, and glycogen...
September 28, 2017: Stem Cells
https://www.readbyqxmd.com/read/28951723/microvesicles-derived-from-indoxyl-sulfate-treated-endothelial-cells-induce-endothelial-progenitor-cells-dysfunction
#18
Andres Carmona, Fatima Guerrero, Paula Buendia, Teresa Obrero, Pedro Aljama, Julia Carracedo
Cardiovascular disease is a major cause of mortality in chronic kidney disease patients. Indoxyl sulfate (IS) is a typical protein-bound uremic toxin that cannot be effectively cleared by conventional dialysis. Increased IS is associated with the progression of chronic kidney disease and development of cardiovascular disease. After endothelial activation by IS, cells release endothelial microvesicles (EMV) that can induce endothelial dysfunction. We developed an in vitro model of endothelial damage mediated by IS to evaluate the functional effect of EMV on the endothelial repair process developed by endothelial progenitor cells (EPCs)...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28950284/overexpression-of-insulin-like-growth-factor-2-in-expanded-endothelial-progenitor-cells-improves-left-ventricular-function-in-experimental-myocardial-infarction
#19
Gabi Demetz, Robert A J Oostendorp, A Melanie Boxberg, Wibke Sitz, Eliane Farrell, Birgit Steppich, Anna-Lena Steinsiek, Martina Rudelius, Ilka Ott
Insulin-like growth factors (IGFs) are mediators of growth hormone-induced metabolic and anabolic actions, but also regulate cell growth, differentiation, and apoptosis and show beneficial effects in acute myocardial ischemia. Since endothelial progenitor cells (EPCs) improve myocardial function after acute myocardial infarction, we sought to investigate whether overexpression of IGF-2 in expanded EPCs (eEPCs) further contributes to improvement in left ventricular function after myocardial infarction. EPCs were isolated from human cord blood and transduced by a retroviral vector expression of IGF-2 (IGF-2 eEPCs) or vector only...
September 27, 2017: Journal of Vascular Research
https://www.readbyqxmd.com/read/28935639/isolation-and-characterization-of-endothelial-to-mesenchymal-transition-cells-in-pulmonary-arterial-hypertension
#20
Toshio Suzuki, Erica J Carrier, Megha H Talati, Anandharajan Rathinasabapathy, Xinping Chen, Rintaro Nishimura, Yuji Tada, Koichiro Tatsumi, James D West
Endothelial-to-mesenchymal transition (EndMT) is a process in which endothelial cells lose polarity and cell-to-cell contacts, and undergo a dramatic remodeling of the cytoskeleton. It has been implicated in initiation and progression of pulmonary arterial hypertension (PAH). However, the characteristics of cells which have undergone EndMT-cells in vivo have not been reported and so remain unclear. To study this, sugen5416 and hypoxia (SuHx)-induced PAH was established in Cdh5-Cre / Gt(ROSA)26Sor(tm4(ACTB-tdTomato,-EGFP)Luo)/J double-transgenic mice, in which GFP was stably expressed in pan-endothelial cells...
September 21, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
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