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Isolation of endothelial progenitor cells

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https://www.readbyqxmd.com/read/28545072/effect-of-vitamin-d-on-endothelial-progenitor-cells-function
#1
Yoav Hammer, Alissa Soudry, Amos Levi, Yeela Talmor-Barkan, Dorit Leshem-Lev, Joel Singer, Ran Kornowski, Eli I Lev
BACKGROUND: Endothelial progenitor cells (EPCs) are a population of bone marrow-derived cells, which have an important role in the process of endothelialization and vascular repair following injury. Impairment of EPCs, which occurs in patients with diabetes, was shown to be related to endothelial dysfunction, coronary artery disease (CAD) and adverse clinical outcomes. Recent evidence has shown that calcitriol, the active hormone of vitamin D, has a favorable impact on the endothelium and cardiovascular system...
2017: PloS One
https://www.readbyqxmd.com/read/28542561/impaired-functional-capacity-of-fetal-endothelial-cells-in-preeclampsia
#2
Lars Brodowski, Jennifer Burlakov, Sarah Hass, Constantin von Kaisenberg, Frauke von Versen-Höynck
OBJECTIVES: Preeclampsia is one of the main contributers to maternal and fetal morbidity and mortality during pregnancy. A history of preeclampsia puts mother and offspring at an increased cardiovascular risk in later life. We hypothesized that at the time of birth functional impairments of fetal endothelial cells can be detected in pregnancies complicated by preeclampsia and that a therapeutic intervention using 1,25 (OH)2 vitamin D3 can reverse the adverse effects of preeclampsia on cell function...
2017: PloS One
https://www.readbyqxmd.com/read/28540031/outgrowing-endothelial-and-smooth-muscle-cells-for-tissue-engineering-approaches
#3
Moritz Kolster, Mathias Wilhelmi, Claudia Schrimpf, Andres Hilfiker, Axel Haverich, Thomas Aper
In recent years, circulating progenitors of endothelial cells and smooth muscle cells were identified in the peripheral blood. In our study, we evaluated the utilization of both cell types isolated and differentiated from peripheral porcine blood in terms for their use for tissue engineering purposes. By means of density gradient centrifugation, the monocyte fraction from porcine blood was separated, split, and cultivated with specific culture media with either endothelial cell growth medium-2 or smooth muscle cell growth medium-2 for the differentiation of endothelial cells or smooth muscle cells...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28535533/malat1-modulates-tgf-%C3%AE-1-induced-endothelial-to-mesenchymal-transition-through-downregulation-of-mir-145
#4
Yin Xiang, Yachen Zhang, Yong Tang, Qianhui Li
BACKGROUND/AIMS: Endothelial-to-mesenchymal transition (EndMT) plays significant roles under various pathological conditions including cardiovascular diseases, fibrosis, and cancer. EndMT of endothelial progenitor cells (EPCs) contributes to neointimal hyperplasia following cell therapy Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a long non-coding RNA (lncRNA) that promotes metastasis and cancer. MicroRNA-145 (miR-145) is a tumor suppressor that has been reported to inhibit SMAD3-mediated epithelial-to-mesenchymal transition (EMT) of cancer cells...
May 25, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28531804/protective-effect-and-mechanism-of-lycopene-on-endothelial-progenitor-cells-epcs-from-type-2-diabetes-mellitus-rats
#5
Yao-Chi Zeng, Li-Sheng Peng, Liyuan Zou, Shu-Fen Huang, Yi Xie, Gui-Ping Mu, Xue-Hui Zeng, Xi-Lin Zhou, Ya-Chi Zeng
Endothelial progenitor cells (EPCs), widely existing in bone marrow and peripheral blood, are involved in the repair of injured vascular endothelium and angiogenesis which are important to diabetic mellitus (DM) patients with vascular complications. The number and the function of EPCs are related to the advanced glycation end products (AGEs) generated in DM patients. Lycopene (Lyc) is an identified natural antioxidant that protects EPCs under the microenvironment of AGEs from damage. However, the underlying mechanism remains unclear...
May 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28528862/crocetin-restores-diabetic-endothelial-progenitor-cell-dysfunction-by-enhancing-no-bioavailability-via-regulation-of-pi3k-akt-enos-and-ros-pathways
#6
Wei Cao, Jinjin Cui, Shufeng Li, Donghui Zhang, Yibo Guo, Qiannan Li, Ying Luan, Xian Liu
AIMS: Endothelial progenitor cell (EPC) dysfunction underlies a critical risk factor in diabetic vascular complications due to function defect in restoring endothelium injury. Crocetin has attracted increasing attention in several vascular-related diseases. In present study, we aimed to explore the role of crocetin in diabetic EPC dysfunction. MAIN METHODS: EPCs were isolated from bone marrow in diabetic mice and identified using the fluorescence staining and flow cytometry...
May 18, 2017: Life Sciences
https://www.readbyqxmd.com/read/28513999/microparticles-of-healthy-origins-improve-endothelial-progenitor-cell-dysfunction-via-microrna-transfer-in-an-atherosclerotic-hamster-model
#7
Nicoleta Alexandru, Eugen Andrei, Loredan Niculescu, Emanuel Dragan, Violeta Ristoiu, Adriana Georgescu
AIM: In this study we aimed: (1) to obtain and functionally characterize the cultures of late endothelial progenitor cells (EPCs) from the animal blood; (2) to investigate the potential beneficial effects of circulating microparticles (MPs) of healthy origins on EPC dysfunctionality in atherosclerosis as well as involved mechanisms. METHODS: Late EPCs were obtained and expanded in culture from peripheral blood isolated from two animal groups: hypertensive-hyperlipidemic (HH) and control (C) hamsters...
May 17, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/28501476/endothelial-cells-are-not-required-for-specification-of-respiratory-progenitors
#8
Jamie A Havrilak, Kristin R Melton, John M Shannon
Crosstalk between mesenchymal and epithelial cells influences organogenesis in multiple tissues, such as lung, pancreas, liver, and the nervous system. Lung mesenchyme comprises multiple cell types, however, and precise identification of the mesenchymal cell type(s) that drives early events in lung development remains unknown. Endothelial cells have been shown to be required for some aspects of lung epithelial patterning, lung stem cell differentiation, and regeneration after injury. Furthermore, endothelial cells are involved in early liver and pancreas development...
May 10, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28499264/initial-clonogenic-potential-of-human-endothelial-progenitor-cells-is-predictive-of-their-further-properties-and-establishes-a-functional-hierarchy-related-to-immaturity
#9
Ségolène Ferratge, Guillaume Ha, Gilles Carpentier, Nassim Arouche, Rümeyza Bascetin, Laurent Muller, Stéphane Germain, Georges Uzan
Endothelial progenitor cells (EPCs) generate in vitro Endothelial Colony Forming Cells (ECFCs) combining features of endothelial and stem/progenitor cells. Their angiogenic properties confer them a therapeutic potential for treating ischemic lesions. They may be isolated from umbilical cord blood (CB-ECFCs) or peripheral adult blood (AB-ECFCs). It is generally accepted that CB-ECFCs are more clonogenic, proliferative and angiogenic than AB-ECFCs. Nevertheless, only a few studies have focused on the functional heterogeneity of CB-ECFCs from different individuals...
May 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28495397/safety-profile-and-long-term-engraftment-of-human-cd31-blood-progenitors-in-bone-tissue-engineering
#10
Hadar Zigdon-Giladi, Rina Elimelech, Gal Michaeli-Geller, Utai Rudich, Eli E Machtei
BACKGROUND: Endothelial progenitor cells (EPCs) participate in angiogenesis and induce favorable micro-environments for tissue regeneration. The efficacy of EPCs in regenerative medicine is extensively studied; however, their safety profile remains unknown. Therefore, our aims were to evaluate the safety profile of human peripheral blood-derived EPCs (hEPCs) and to assess the long-term efficacy of hEPCs in bone tissue engineering. METHODS: hEPCs were isolated from peripheral blood, cultured and characterized...
May 8, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28471299/interleukin-10-deficiency-impairs-reparative-properties-of-bone-marrow-derived-endothelial-progenitor-cell-exosome-function
#11
Yujia Yue, Venkata Ns Garikipati, Suresh K Verma, David A Goukassian, Raj Kishore
Endothelial progenitor cell (EPC)-based therapy has immense potential to promote cardiac neovascularization and to attenuate ischemic injury. Functional benefits of EPCs and other adult stem cell therapies largely involve paracrine mechanisms and exosomes secreted by stem cells are emerging as pivotal paracrine entity of stem/progenitor cells. However, modest outcomes after EPC/stem cells based clinical trials suggest that stem cell/exosome function might be modulated by stimuli they encounter in ischemic tissues including systemic inflammation...
May 4, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28464921/human-adult-mesangiogenic-progenitor-cells-reveal-an-early-angiogenic-potential-which-is-lost-after-mesengenic-differentiation
#12
Marina Montali, Francesca M Panvini, Serena Barachini, Francesca Ronca, Vittoria Carnicelli, Stefano Mazzoni, Iacopo Petrini, Simone Pacini
BACKGROUND: Mesangiogenic progenitor cells (MPCs) have shown the ability to differentiate in-vitro toward mesenchymal stromal cells (MSCs) as well as angiogenic potential. MPCs have so far been described in detail as progenitors of the mesodermal lineage and appear to be of great significance in tissue regeneration and in hemopoietic niche regulation. On the contrary, information regarding the MPC angiogenic process is still incomplete and requires further clarification. In particular, genuine MPC angiogenic potential should be confirmed in-vivo...
May 2, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28457994/increase-in-acid-sphingomyelinase-level-in-human-retinal-endothelial-cells-and-cd34-circulating-angiogenic-cells-isolated-from-diabetic-individuals-is-associated-with-dysfunctional-retinal-vasculature-and-vascular-repair-process-in-diabetes
#13
Nermin Kady, Yuanqing Yan, Tatiana Salazar, Qi Wang, Harshini Chakravarthy, Chao Huang, Eleni Beli, Svetlana Navitskaya, Maria Grant, Julia Busik
BACKGROUND: Diabetic retinopathy is a microvascular disease that results from retinal vascular degeneration and defective repair due to diabetes-induced endothelial progenitor dysfunction. OBJECTIVE: Understanding key molecular factors involved in vascular degeneration and repair is paramount for developing effective diabetic retinopathy treatment strategies. We propose that diabetes-induced activation of acid sphingomyelinase (ASM) plays essential role in retinal endothelial and CD34(+) circulating angiogenic cell (CAC) dysfunction in diabetes...
May 2017: Journal of Clinical Lipidology
https://www.readbyqxmd.com/read/28452591/donor-dependent-variances-of-human-adipose-derived-stem-cells-in-respect-to-the-in-vitro-endothelial-cell-differentiation-capability
#14
Jascha Ell, Sybille Regn, Anna-Maria Buchberger, Achim von Bomhard, Thomas Stark, Jan-Thorsten Schantz, Katharina Storck
Human adipose-derived stem cells (ASC) have been shown to differentiate into mature adipocytes and to play an important role in creating the vasculature, necessary for white adipose tissue to function. To study the stimulatory capacity of ASC on endothelial progenitor cells we used a commercially available co-culture system (V2a - assay). ASC, isolated from lipoaspirates of 18 healthy patients, were co-cultured for 13 d on endothelial progenitor cells. Using anti CD31 immunostaining, cells that had undergone endothelial differentiation were quantified after the defined co-cultivation period...
January 2, 2017: Adipocyte
https://www.readbyqxmd.com/read/28450224/pinocembrin-ex-vivo-preconditioning-improves-the-therapeutic-efficacy-of-endothelial-progenitor-cells-in-monocrotaline-induced-pulmonary-hypertension-in-rats
#15
Lamiaa A Ahmed, Sherine M Rizk, Shohda A El-Maraghy
Pulmonary hypertension is still not curable and the available current therapies can only alleviate symptoms without hindering the progression of disease. The present study was directed to investigate the possible modulatory effect of pinocembrin on endothelial progenitor cells transplanted in monocrotaline-induced pulmonary hypertension in rats. Pulmonary hypertension was induced by a single subcutaneous injection of monocrotaline (60 mg/kg). Endothelial progenitor cells was in vitro preconditioned with pinocembrin (25 mg/L) for 30 min before being i...
April 24, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28450163/murine-hemogenic-endothelial-precursors-display-heterogeneous-hematopoietic-potential-ex%C3%A2-vivo
#16
Miguel Ganuza, Brandon Hadland, Ashley Chabot, Chen Li, Guolian Kang, Irwin Bernstein, Shannon McKinney-Freeman
Hematopoietic stem and progenitor cells (HSPCs) sustain life-long hematopoiesis and are first detected in the embryo by transplantation at embryonic day 10.5 (E10.5). HSPCs are mesodermal in origin and ultimately emerge from a subset of arterial endothelium (i.e., hemogenic endothelium [HE]), which is highly concentrated in the aorta-gonad-mesonephros region of the midgestation embryo. Here, we used clonal ex vivo assays, in which endothelial cells isolated from the midgestation aorta and vitelline and umbilical arteries are co-cultured on supportive stroma, to show that only about 0...
April 24, 2017: Experimental Hematology
https://www.readbyqxmd.com/read/28446444/concurrent-isolation-of-three-distinct-cardiac-stem-cell-populations-from-a-single-human-heart-biopsy
#17
Megan M Monsanto, Kevin S White, Taeyong Kim, Bingyan J Wang, Kristina Fisher, Kelli Ilves, Farid G Khalafalla, Alexandria Casillas, Kathleen Broughton, Sadia Mohsin, Walter P Dembitsky, Mark A Sussman
Rationale: The relative actions and synergism between distinct myocardial-derived stem cell populations remains obscure. Ongoing debates regarding optimal cell population(s) for treatment of heart failure prompted implementation of a protocol for isolation of multiple stem cell populations from a single myocardial tissue sample to develop new insights for achieving myocardial regeneration. Objective: Establish a robust cardiac stem cell isolation and culture protocol to consistently generate three distinct stem cell populations from a single human heart biopsy...
April 26, 2017: Circulation Research
https://www.readbyqxmd.com/read/28444128/low-dose-ionizing-radiation-induces-therapeutic-neovascularization-in-a-pre-clinical-model-of-hindlimb-ischemia
#18
Augusto Ministro, Paula de Oliveira, Raquel J Nunes, André Dos Santos Rocha, Adriana Correia, Tânia Carvalho, José Rino, Pedro Faísca, Jorg D Becker, João Goyri-O'Neill, Filomena Pina, Esmeralda Poli, Bruno Silva-Santos, Fausto Pinto, Marc Mareel, Karine Serre, Susana Constantino Rosa Santos
Aims: We have previously shown that low-dose ionizing radiation (LDIR) induces angiogenesis but there is no evidence that it induces neovascularization in the setting of peripheral arterial disease. Here, we investigated the use of LDIR as an innovative and non-invasive strategy to stimulate therapeutic neovascularization using a model of experimentally induced hindlimb ischemia (HLI). Methods and results: After surgical induction of unilateral HLI, both hindlimbs of female C57BL/6 mice were sham-irradiated or irradiated with four daily fractions of 0...
June 1, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28440414/thymosin-%C3%AE-4-promotes-the-survival-and-angiogenesis-of-transplanted-endothelial-progenitor-cells-in-the-infarcted-myocardium
#19
Zhe Quan, Qiang-Li Wang, Pei Zhou, Guo-Dong Wang, Yu-Zhen Tan, Hai-Jie Wang
The survival of transplanted stem cells in ischemic tissue is poor. In the present study, the effects of thymosin β4 (Tβ4) on the survival and angiogenesis of endothelial progenitor cells (EPCs) and improvement in cardiac functions after transplantation of Tβ4-treated EPCs in the infarcted myocardium were investigated. EPCs were isolated from bone marrow of adult male rats and incubated in Endothelial Basal Medium-2. Then the cells were treated with Tβ4 at different concentrations (0.05, 0.1 and 0.2 µM), and cells incubated with DMEM were set as controls...
June 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28415797/rab7-gtpase-controls-lipid-metabolic-signaling-in-myeloid-derived-suppressor-cells
#20
Xinchun Ding, Wenjing Zhang, Ting Zhao, Cong Yan, Hong Du
Lysosomal acid lipase (LAL) is a critical neutral lipid metabolic enzyme that regulates metabolic reprogramming in myeloid-derived suppressor cells (MDSCs) through over-activation of mammalian target of rapamycin (mTOR). Affymetrix GeneChip microarray analysis of MDSCs from LAL deficient mouse (lal-/-) revealed upregulation of Rab7 GTPase protein, which belongs to a superfamily of small-molecular-weight GTPase known to regulate intracellular membrane trafficking from early to late endosomes and lysosomes. Here, the physical protein-protein interaction between Rab7 GTPase and mTOR has been detected by co-immunoprecipitation in the cell extract of wild type HD1A and lal-/- MDSC-like HD1B myeloid cell lines...
May 2, 2017: Oncotarget
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