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Isolation of endothelial progenitor cells

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https://www.readbyqxmd.com/read/28415797/rab7-gtpase-controls-lipid-metabolic-signaling-in-myeloid-derived-suppressor-cells
#1
Xinchun Ding, Wenjing Zhang, Ting Zhao, Cong Yan, Hong Du
Lysosomal acid lipase (LAL) is a critical neutral lipid metabolic enzyme that regulates metabolic reprogramming in myeloid-derived suppressor cells (MDSCs) through over-activation of mammalian target of rapamycin (mTOR). Affymetrix GeneChip microarray analysis of MDSCs from LAL deficient mouse (lal-/-) revealed upregulation of Rab7 GTPase protein, which belongs to a superfamily of small-molecular-weight GTPase known to regulate intracellular membrane trafficking from early to late endosomes and lysosomes. Here, the physical protein-protein interaction between Rab7 GTPase and mTOR has been detected by co-immunoprecipitation in the cell extract of wild type HD1A and lal-/- MDSC-like HD1B myeloid cell lines...
March 16, 2017: Oncotarget
https://www.readbyqxmd.com/read/28402508/preserved-endothelial-progenitor-cell-angiogenic-activity-in-african-american-essential-hypertensive-patients
#2
Seo Rin Kim, Alfonso Eirin, Sandra M S Herrmann, Ahmed Saad, Luis A Juncos, Amir Lerman, Stephen C Textor, Lilach O Lerman
Background.: African American (AA) subjects with essential hypertension (EH) have greater inflammation and cardiovascular complications than Caucasian EH. An impaired endogenous cellular repair system may exacerbate vascular injury in hypertension, yet whether these differ between AA EH and Caucasian EH remains unknown. Vascular repair by circulating endothelial progenitor cells (EPCs) is controlled by regulators of EPC mobilization, homing, adhesion and new vessel formation, but can be hindered by various cytokines...
April 10, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/28386230/role-of-nadph-oxidase-4-in-human-endothelial-progenitor-cells
#3
Nora Y Hakami, Amaresh K Ranjan, Anandwardhan A Hardikar, Greg J Dusting, Hitesh M Peshavariya
Introduction: Endothelial progenitor cells (EPCs) display a unique ability to promote angiogenesis and restore endothelial function in injured blood vessels. NADPH oxidase 4 (NOX4)-derived hydrogen peroxide (H2O2) serves as a signaling molecule and promotes endothelial cell proliferation and migration as well as protecting against cell death. However, the role of NOX4 in EPC function is not completely understood. Methods: EPCs were isolated from human saphenous vein and mammary artery discarded during bypass surgery...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28380641/-development-of-an-innovative-cell-isolation-method-for-the-investigation-of-breast-cancer-pathogenesis-and-angiogenesis-for-experimental-in-vitro-and-in-vivo-assays
#4
Annika Weigand, Kereshmeh Tasbihi, Pamela L Strissel, Reiner Strick, Raymund E Horch, Anja M Boos
Breast cancer is the world's most common cancer among women. Autologous lipotransfer is increasingly used for breast reconstruction following surgical removal of the tumour. In cell-assisted lipotransfer, the transplant is enriched with stem cells from adipose tissue (ADSC). Despite positive clinical results, there are some concerns regarding oncological safety due to transplanted stem cells. To date there are only a few breast cancer studies using primary cells from the same patient to enable further investigation into the complexity of cell-cell interactions in breast cancer in an experimental setting...
April 5, 2017: Handchirurgie, Mikrochirurgie, Plastische Chirurgie
https://www.readbyqxmd.com/read/28380401/microlens-topography-combined-with-vascular-endothelial-growth-factor-induces-endothelial-differentiation-of-human-mesenchymal-stem-cells-into-vasculogenic-progenitors
#5
Marek Kukumberg, Jia Yi Yao, Dawn J H Neo, Evelyn K F Yim
Cell therapy for vascular damage has been showing promises as alternative therapy for endothelial dysfunctions since the discovery of the endothelial progenitor cells (EPCs). However, isolated EPCs from peripheral blood yield low cell amounts and alternative cell source must be explored. The aim of this study was to investigate the influence of topography on the endothelial differentiation of an alternative cell source - human mesenchymal stem cells (hMSCs) from bone marrow. Utilizing the MultiARChitecture (MARC) chip, a systematic screening of variety of patterned surfaces and different medium compositions was performed...
July 2017: Biomaterials
https://www.readbyqxmd.com/read/28374977/hypoxic-preconditioning-induced-autophagy-enhances-survival-of-engrafted-endothelial-progenitor-cells-in-ischaemic-limb
#6
Pei Zhou, Yu-Zhen Tan, Hai-Jie Wang, Guo-Dong Wang
Recent clinical studies have suggested that endothelial progenitor cells (EPCs) transplantation provides a modest benefit for treatment of the ischaemic diseases such as limb ischaemia. However, cell-based therapies have been limited by poor survival of the engrafted cells. This investigation was designed to establish optimal hypoxia preconditioning and evaluate effects of hypoxic preconditioning-induced autophagy on survival of the engrafted EPCs. Autophagy of CD34(+) VEGFR-2(+) EPCs isolated from rat bone marrow increased after treatment with 1% O2 ...
April 4, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28372279/circulating-endothelial-progenitors-in-vascular-repair
#7
S Ferratge, J Boyer, N Arouch, F Chevalier, G Uzan
Endothelial Colony Forming Cells (ECFCs) are obtained in culture from Circulating Endothelial Progenitor Cells. They display all characteristics of endothelial cells and they display stem cells features. Cord blood-derived ECFCs (CB-ECFCs) have a high clonogenic and proliferative potentials, and exhibit vascular repair capabilities useful for the treatment of ischemic diseases. However, the link between immaturity and functional properties of CB-ECFCs is still poorly defined. We showed that these cells have a high clonogenic potential and are capable to be efficiently reprogrammed into induced pluripotent stem cells...
2017: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/28367090/high-quality-in-vitro-expansion-of-human-endothelial-progenitor-cells-of-human-umbilical-vein-origin
#8
Yan Mou, Zhen Yue, Haiying Zhang, Xu Shi, Mingrui Zhang, Xiaona Chang, Hang Gao, Ronggui Li, Zonggui Wang
The limited availability of qualified endothelial progenitor cells (EPCs) is a major challenge for regenerative medicine. In the present study, we isolated human EPCs from human umbilical vein endothelial cells (HUVECs) by using magnetic micro-beads coated with an antibody against human CD34. Flow cytometric assay showed that majority of these cells expressed VEGFR2 (KDR), CD34 and CD133, three molecular markers for early EPCs. It was also found that a bioreactor micro-carrier cell culture system (bio-MCCS) was superior to dish culture for in vitro expansion of EPCs...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/28361046/endothelial-progenitor-cells-of-the-human-placenta-and-fetoplacental-circulation-a-potential-link-to-fetal-neonatal-and-long-term-health
#9
REVIEW
Diane L Gumina, Emily J Su
The fetoplacental circulation plays a key role in both short- and long-term outcomes, and aberrant flow indices as manifested by abnormal fetal Doppler velocimetry within this compartment have been associated with significant adverse consequences. These include fetal growth restriction, which often coexists with preeclampsia, and long-lasting medical issues as a result of both the underlying pathology and prematurity such as bronchopulmonary dysplasia, chronic lung disease, and neurodevelopmental delay. Furthermore, it is also clear that exposure to an abnormal in utero environment increases risk for long-term, adulthood issues such as cardiovascular disease...
2017: Frontiers in Pediatrics
https://www.readbyqxmd.com/read/28360519/intratracheal-transplantation-of-endothelial-progenitor-cells-attenuates-smoking-induced-copd-in-mice
#10
Zhihui Shi, Yan Chen, Jun Cao, Huihui Zeng, Yue Yang, Ping Chen, Hong Luo, Hong Peng, Shan Cai, Chaxiang Guan
BACKGROUND: Endothelial progenitor cells (EPCs) might play a protective role in COPD. The aim of this study was to investigate whether intratracheal allogeneic transplantation of bone-marrow-derived EPCs would attenuate the development of smoking-induced COPD in mice. METHODS: Isolated mononuclear cells from the bone marrow of C57BL/6J mice were cultured in endothelial cell growth medium-2 for 10 days, yielding EPCs. A murine model of COPD was established by passive 90-day exposure of cigarette smoke...
2017: International Journal of Chronic Obstructive Pulmonary Disease
https://www.readbyqxmd.com/read/28352314/vegf-treatment-promotes-bone-marrow-derived-cxcr4-mesenchymal-stromal-stem-cell-differentiation-into-vessel-endothelial-cells
#11
Qiming Li, Shudong Xia, Hanyun Fang, Jiansheng Pan, Yinfeng Jia, Gang Deng
Stem/progenitor cells serve an important role in the process of blood vessel repair. However, the mechanism of vascular repair mediated by C-X-C chemokine receptor type 4-positive (CXCR4(+)) bone marrow-derived mesenchymal stem cells (BMSCs) following myocardial infarction remains unclear. The aim of the present study was to investigate the effects of vascular endothelial growth factor (VEGF) on vessel endothelial differentiation from BMSCs. CXCR4(+) BMSCs were isolated from the femoral bone marrow of 2-month-old mice and the cells were treated with VEGF...
February 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28346707/periurethral-muscle-derived-mononuclear-cell-injection-improves-urethral-sphincter-restoration-in-rats
#12
Marcelo Pitelli Turco, Alex Balduino de Souza, Isida de Campos Sousa, Paula Fratini, Mariana Matera Veras, Marcio Nogueira Rodrigues, José de Bessa, Marina Pandolphi Brolio, Katia Ramos Moreira Leite, Homero Bruschini, Miguel Srougi, Maria Angélica Miglino, Cristiano Mendes Gomes
AIMS: Investigate the effect of a novel cell-based therapy with skeletal muscle-derived mononuclear cells (SMDMCs) in a rat model of stress urinary incontinence. METHODS: Male Wistar-Kyoto rats' hind limb muscles were enzymatically dissociated, and SMDMCs were isolated without needing expansion. The cell population was characterized. Twenty female rats underwent urethrolysis. One week later, 10 rats received periurethral injection of 10(6) cells (SMDMC group), and 10 rats received saline injections (Saline group)...
March 27, 2017: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/28317180/anti-acsa-2-defines-a-novel-monoclonal-antibody-for-prospective-isolation-of-living-neonatal-and-adult-astrocytes
#13
Christina G Kantzer, Camille Boutin, Ina D Herzig, Carolina Wittwer, Sandy Reiß, Marie Catherine Tiveron, Jan Drewes, Thomas D Rockel, Stefanie Ohlig, Jovica Ninkovic, Harold Cremer, Sandra Pennartz, Melanie Jungblut, Andreas Bosio
Astrocytes are the most abundant cell type of the central nervous system and cover a broad range of functionalities. We report here the generation of a novel monoclonal antibody, anti-astrocyte cell surface antigen-2 (Anti-ACSA-2). Flow cytometry, immunohistochemistry and immunocytochemistry revealed that Anti-ACSA-2 reacted specifically with a not yet identified glycosylated surface molecule of murine astrocytes at all developmental stages. It did not show any labeling of non-astroglial cells such as neurons, oligodendrocytes, NG2(+) cells, microglia, endothelial cells, leukocytes, or erythrocytes...
March 20, 2017: Glia
https://www.readbyqxmd.com/read/28303468/human-very-small-embryonic-like-cells-support-vascular-maturation-and-therapeutic-revascularization-induced-by-endothelial-progenitor-cells
#14
Coralie L Guerin, Elisa Rossi, Bruno Saubamea, Audrey Cras, Virginie Mignon, Jean-Sébastien Silvestre, David M Smadja
Very small embryonic-like stem cells (VSELs) are major pluripotent stem cells defined as cells of small size being Lineage- negative, CD133-positive, and CD45-negative. We previously described that human bone marrow VSELs were able to differentiate into endothelial cells and promoted post-ischemic revascularization in mice with surgically induced critical limb ischemia. In the present work, we isolated bone marrow VSELs from patients with critical limb ischemia and studied their ability to support endothelial progenitor cells therapeutic capacity and revascularization potential...
March 16, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28302184/cytokine-free-directed-differentiation-of-human-pluripotent-stem-cells-efficiently-produces-hemogenic-endothelium-with-lymphoid-potential
#15
Yekaterina Galat, Svetlana Dambaeva, Irina Elcheva, Aaruni Khanolkar, Kenneth Beaman, Philip M Iannaccone, Vasiliy Galat
BACKGROUND: The robust generation of human hematopoietic progenitor cells from induced or embryonic pluripotent stem cells would be beneficial for multiple areas of research, including mechanistic studies of hematopoiesis, the development of cellular therapies for autoimmune diseases, induced transplant tolerance, anticancer immunotherapies, disease modeling, and drug/toxicity screening. Over the past years, significant progress has been made in identifying effective protocols for hematopoietic differentiation from pluripotent stem cells and understanding stages of mesodermal, endothelial, and hematopoietic specification...
March 17, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28287533/isolation-of-endothelial-progenitor-cells-from-healthy-volunteers-and-their-migratory-potential-influenced-by-serum-samples-after-cardiac-surgery
#16
Christoph Emontzpohl, David Simons, Sandra Kraemer, Andreas Goetzenich, Gernot Marx, Jürgen Bernhagen, Christian Stoppe
Endothelial progenitor cells (EPCs) are recruited from the bone marrow under pathological conditions like hypoxia and are crucially involved in the neovascularization of ischemic tissues. The origin, classification and characterization of EPCs are complex; notwithstanding, two prominent sub-types of EPCs have been established: so-called "early" EPCs (subsequently referred to as early-EPCs) and late-outgrowth EPCs (late-EPCs). They can be classified by biological properties as well as by their appearance during in vitro culture...
February 14, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28247345/isolation-culture-and-immunostaining-of-skeletal-muscle-myofibers-from-wildtype-and-nestin-gfp-mice-as-a-means-to-analyze-satellite-cell
#17
Pascal Stuelsatz, Paul Keire, Zipora Yablonka-Reuveni
Multinucleated myofibers, the functional contractile units of adult skeletal muscle, harbor mononuclear Pax7(+) myogenic progenitors on their surface between the myofiber basal lamina and plasmalemma. These progenitors, known as satellite cells, are the primary myogenic stem cells in adult muscle. This chapter describes our laboratory protocols for isolating, culturing, and immunostaining intact myofibers from mouse skeletal muscle as a means for studying satellite cell dynamics. The first protocol discusses myofiber isolation from the flexor digitorum brevis (FDB) muscle...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28244038/flow-cytometric-isolation-and-differentiation-of-adipogenic-progenitor-cells-into-brown-and-brite-beige-adipocytes
#18
Jochen Steinbring, Antonia Graja, Anne-Marie Jank, Tim J Schulz
Aside from mature adipocytes, adipose tissue harbors several distinct cell populations including immune cells, endothelial cells, and adipogenic progenitor cells (AdPCs). AdPCs represent the reservoir of regenerative cells that replenishes adipocytes during normal cellular turnover and during times of increased demand for triglyceride-storage capacity. The worldwide increase in pathologies associated with the metabolic syndrome, such as obesity and type-2 diabetes, has heightened public and scientific interest in adipose tissues and the cell biological processes of adipose tissue formation and function...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28229409/endothelial-progenitor-cells-procedure-for-cell-isolation-and-applications
#19
Garikipati V N Srikanth, Raj Kishore
Bone marrow endothelial progenitor cells (EPCs) have shown a great promise to promote ischemic tissue neovascularization and to attenuate ischemic injury in a variety of animal models, which led to EPC-based clinical trials that yielded modest but promising results. Some of the variables in the use of EPCs relate to their differential isolation and characterization protocols since the EPC literature does not identify a unique marker for these vascular progenitors. In this chapter, we present step-by-step protocols for the isolation of EPCs, their characterization and culture conditions, and their potential use in basic and clinical research...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28228349/identification-of-prdm-genes-in-human-corneal-endothelium
#20
Kostadin Rolev, G O'Donovan Dominic, Christiana Georgiou, Rajan Madhavan, Alexandra Chittka
Corneal endothelial cells (CECs) are essential for maintaining corneal stromal hydration and ensuring its transparency, which is necessary for normal vision. Dysfunction of CECs leads to stromal decompensation, loss of transparency and corneal blindness. Corneal endothelium has low proliferative potential compared to surface epithelial cells leading to poor regeneration of CEC following injury or age related decline in cellular density. The mechanisms which control proliferation and differentiation of neural crest derived CEC progenitors are yet to be clearly elucidated...
February 19, 2017: Experimental Eye Research
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