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Isolation of endothelial progenitor cells

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https://www.readbyqxmd.com/read/29771429/the-relationship-between-number-and-function-of-epcs-and-concentration-of-vegf165-and-sdf-1-in-coronary-artery-spasm
#1
Z-Q Sheng, Y-F Li, K-L Zheng, H-H Lu, J Xie, H Wu, B Xu
OBJECTIVE: We aimed at investigating the functions of endothelial progenitor cells (EPCs) in the pathophysiology of coronary artery spasm (CAS) and coronary artery disease (CAD), as well as to evaluate the correlation of these diseases with the number and function of EPCs, the plasma concentration of vascular endothelial growth factor 165 (VEGF165) and stromal cell-derived factor 1 (SDF-1). PATIENTS AND METHODS: Participants were recruited into three groups, CAS, CAD and the control...
May 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29760742/correlation-between-therapeutic-efficacy-of-cd34-cell-treatment-and-directed-in-vivo-angiogenesis-in-patients-with-end-stage-diffuse-coronary-artery-disease
#2
Tien-Hung Huang, Cheuk-Kwan Sun, Yi-Ling Chen, Pei-Hsun Sung, Chi-Hsiang Chu, Mel S Lee, Yuan-Ping Lin, Hon-Kan Yip, Fan-Yen Lee
Background: This study was aimed at testing the association between the therapeutic efficacy of CD34+ cell treatment in patients with end-stage diffuse coronary artery disease as reflected in angiographic grading and results of directed in vivo angiogenesis assay (DIVAA) on their isolated peripheral blood mononuclear cell- (PBMC-) derived endothelial progenitor cells (EPCs). Methods: Angiographic grades (0: <5%; 1: 5-35%; 2: 35-75%; 3: >75%) which presented the improvement of vessel density pre- and post-CD34+ treatment were given to 30 patients with end-stage diffuse coronary artery disease having received CD34+ cell treatment...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29755107/kai1-cd82-genetically-engineered-endothelial-progenitor-cells-inhibit-metastasis-of-human-nasopharyngeal-carcinoma-in-a-mouse-model
#3
Gengming Wang, Lei Zhang, Yan Zhou, Qian Sun, Hongbo Xu, Feng Cai, Ping Xiang, Zhendong Chen, Hao Jiang
BACKGROUND Endothelial progenitor cells (EPCs) are regarded as promising targeted vectors for delivering therapeutic genes or agents in cancer therapy. The purpose of this study was to investigate the role of intravenously administered KAI1/CD82 genetically transduced EPCs in the tumorigenesis and metastasis of nasopharyngeal carcinoma (NPC). MATERIAL AND METHODS EPCs were isolated from human umbilical cord blood, expanded in culture, and stably transduced with lentiviral vectors expressing KAI1/CD82. The KAI1/CD82 EPCs were injected intravenously into nude mice bearing human NPC xenografts...
May 14, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29737200/human-peripheral-blood-mononuclear-cells-incubated-in-vasculogenic-conditioning-medium-dramatically-improve-ischemia-reperfusion-acute-kidney-injury-in-mice
#4
Takayasu Ohtake, Shuzo Kobayashi, Shimon Slavin, Yasuhiro Mochida, Kunihiro Ishioka, Hidekazu Moriya, Sumi Hidaka, Ryo Matsuura, Maki Sumida, Daisuke Katagiri, Eisei Noiri, Kayoko Okada, Hiroshi Mizuno, Rica Tanaka
Acute kidney injury (AKI) is a major clinical problem that still has no established treatment. We investigated the efficacy of cultured human peripheral blood mononuclear cells (PBMNCs) for AKI. Ischemia/reperfusion injury (IRI) was used to induce AKI in male nonobese diabetic (NOD/severe combined immunodeficiency) mice aged 7 to 8 wk. PBMNCs were isolated from healthy volunteers and were subjected to quality and quantity controlled (QQc) culture for 7 d in medium containing stem cell factor, thrombopoietin, Flt-3 ligand, vascular endothelial growth factor, and interleukin 6...
January 1, 2018: Cell Transplantation
https://www.readbyqxmd.com/read/29717657/bone-marrow-cd133-stem-cells-ameliorate-visual-dysfunction-in-streptozotocin-induced-diabetic-mice-with-early-diabetic-retinopathy
#5
Liyuan Rong, Xianliang Gu, Jing Xie, Yuxiao Zeng, Qiyou Li, Siyu Chen, Ting Zou, Langyue Xue, Haiwei Xu, Zheng Qin Yin
Diabetic retinopathy (DR), one of the leading causes of vision loss worldwide, is characterized by neurovascular disorders. Emerging evidence has demonstrated retinal neurodegeneration in the early pathogenesis of DR, and no treatment has been developed to prevent the early neurodegenerative changes that precede detectable microvascular disorders. Bone marrow CD133+ stem cells with revascularization properties exhibit neuroregenerative potential. However, whether CD133+ cells can ameliorate the neurodegeneration at the early stage of DR remains unclear...
January 1, 2018: Cell Transplantation
https://www.readbyqxmd.com/read/29708992/genetic-profiling-of-young-and-aged-endothelial-progenitor-cells-in-hypoxia
#6
Tzu-Wei Wu, Chun-Chieh Liu, Chung-Lieh Hung, Chih-Hsien Yen, Yih-Jer Wu, Li-Yu Wang, Hung-I Yeh
Age is a major risk factor for diseases caused by ischemic hypoxia, such as stroke and coronary artery disease. Endothelial progenitor cells (EPCs) are the major cells respond to ischemic hypoxia through angiogenesis and vascular remodeling. However, the effect of aging on EPCs and their responses to hypoxia are not well understood. CD34+ EPCs were isolated from healthy volunteers and aged by replicative senescence, which was to passage cells until their doubling time was twice as long as the original cells...
2018: PloS One
https://www.readbyqxmd.com/read/29702753/curcumin-reverses-diabetes-induced-endothelial-progenitor-cell-dysfunction-by-enhancing-mnsod-expression-and-activity-in-vitro-and-in-vivo
#7
Sheetal Kadam, Meghana Kanitkar, Kadambari Dixit, Rucha Deshpande, Vasudevan Seshadri, Vaijayanti Kale
Diabetes mellitus (DM) causes dysfunction of endothelial progenitor cells (EPCs), resulting in impaired wound healing. EPC therapy is a potential substitute to the current treatments of chronic wounds. Since EPCs isolated from diabetic patients are dysfunctional, and therefore pose an obstacle in their efficacious employment in autologous cell therapy, a strategy to rescue them prior to transplantation would be expected to improve the efficacy of autologous cell therapy multi-fold. With compromised ROS scavenging ability being the main cause of EPC Dysfunction (EPCD), ROS scavengers are likely to reverse or rescue EPC dysfunction...
April 27, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29681541/differentiation-of-human-pluripotent-stem-cells-into-functional-endothelial-cells-in-scalable-suspension-culture
#8
Ruth Olmer, Lena Engels, Abdulai Usman, Sandra Menke, Muhammad Nasir Hayat Malik, Frank Pessler, Gudrun Göhring, Dorothee Bornhorst, Svenja Bolten, Salim Abdelilah-Seyfried, Thomas Scheper, Henning Kempf, Robert Zweigerdt, Ulrich Martin
Endothelial cells (ECs) are involved in a variety of cellular responses. As multifunctional components of vascular structures, endothelial (progenitor) cells have been utilized in cellular therapies and are required as an important cellular component of engineered tissue constructs and in vitro disease models. Although primary ECs from different sources are readily isolated and expanded, cell quantity and quality in terms of functionality and karyotype stability is limited. ECs derived from human induced pluripotent stem cells (hiPSCs) represent an alternative and potentially superior cell source, but traditional culture approaches and 2D differentiation protocols hardly allow for production of large cell numbers...
April 17, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29680777/stromal-cells-telocytes-and-endothelial-progenitors-in-the-perivascular-niches-of-the-trigeminal-ganglion
#9
M C Rusu, V S Mănoiu, D Creţoiu, S M Creţoiu, A D Vrapciu
Stromal cells/telocytes (SCs/TCs) were recently described in the human adult trigeminal ganglion (TG). As some markers are equally expressed in SCs/TCs and endothelial cells, we hypothesized that a subset of the TG SCs/TCs is in fact represented by endothelial progenitor cells of a myelomonocytic origin. This study aimed to evaluate whether the interstitial cells of the human adult TG correlate with the myelomonocytic lineage. We used primary antibodies for c-erbB2/HER-2, CD31, nestin, CD10, CD117/c-kit, von Willebrand factor (vWF), CD34, Stro-1, CD146, α-smooth muscle actin (α-SMA), CD68, VEGFR-2 and cytokeratin 7 (CK7)...
April 19, 2018: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/29675374/comparison-of-three-fluorescence-labeling-and-tracking-methods-of-endothelial-progenitor-cells-in-laser-injured-retina
#10
Hui Shi, Xin-Rui Wang, Ming-Chao Bi, Wei Yang, Dan Wang, Hai-Le Liu, Ling-Ling Liang, Xiao-Hong Li, Qian Hao, Zhi-Hua Cui, E Song
AIM: To compare three kinds of fluorescent probes for in vitro labeling and in vivo tracking of endothelial progenitor cells (EPCs) in a mouse model of laser-induced retinal injury. METHODS: EPCs were isolated from human umbilical cord blood mononuclear cells and labeled with three different fluorescent probes: 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE), 1,1'-dilinoleyl-3,3,3',3'-tetramethylindo-carbocyanine perchlorate linked acetylated low-density lipoprotein (DiI-AcLDL), and green fluorescent protein (GFP)...
2018: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/29655982/polyprenylated-polycyclic-acylphloroglucinol-angiogenesis-inhibitor-from-garcinia-multiflora
#11
Lin-Yang Cheng, Chun-Lin Chen, Yueh-Hsiung Kuo, Tsung-Hsien Chang, I-Wei Lin, Shih-Wei Wang, Mei-Ing Chung, Jih-Jung Chen
A new polyprenylated polycyclic acylphloroglucinol, garcimultiflorone K (1), has been isolated from the stems of Garcinia multiflora, together with two known compounds, garcimultiflorone A (2) and garcimultiflorone B (3). The structure of new compound 1 was determined through spectroscopic methods including 1D and 2D NMR and MS analyses. The anti-angiogenic and anti-cancer effects of compounds 1-3 were evaluated in human endothelial progenitor cells (EPCs) and cancer cells. Of these, garcimultiflorone K (1) displayed the most potent anti-angiogenic property by suppressing cell growth and tube formation of EPCs...
April 6, 2018: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29615783/isolation-and-characterisation-of-cd9-positive-pituitary-adult-stem-progenitor-cells-in-rats
#12
Kotaro Horiguchi, Ken Fujiwara, Saishu Yoshida, Takashi Nakakura, Ken Arae, Takehiro Tsukada, Rumi Hasegawa, Shu Takigami, Shunji Ohsako, Takashi Yashiro, Takako Kato, Yukio Kato
S100β protein and SOX2-double positive (S100β/SOX2-positive) cells have been suggested to be adult pituitary stem/progenitor cells exhibiting plasticity and multipotency. The aim of the present study was to isolate S100β/SOX2-positive cells from the adult anterior lobes of rats using a specific antibody against a novel membrane marker and to study their characteristics in vitro. We found that cluster of differentiation (CD) 9 is expressed in the majority of adult rat S100β/SOX2-positive cells, and we succeeded in isolating CD9-positive cells using an anti-CD9 antibody with a pluriBead-cascade cell isolation system...
April 3, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29601599/vcam-1-expression-is-upregulated-by-cd34-cd133-stem-cells-derived-from-septic-patients
#13
Christian Patry, Christoph Remmé, Christian Betzen, Burkhard Tönshoff, Benito A Yard, Grietje Beck, Neysan Rafat
CD34+/CD133+- cells are a bone marrow derived stem cell population, which presumably contain vascular progenitor cells and are associated with improved vascular repair. In this study, we investigated whether the adhesion molecules ICAM-1 (intercellular adhesion molecule-1), VCAM-1 (vascular adhesion molecule-1), E-selectin und L-selectin, which are involved in homing of vascular stem cells, are upregulated by CD34+/CD133+-stem cells from septic patients and would be associated with improved clinical outcome...
2018: PloS One
https://www.readbyqxmd.com/read/29583085/functional-heterogeneity-of-endothelial-cells-derived-from-human-pluripotent-stem-cells
#14
Saritha S D'Souza, Akhilesh Kumar, Igor I Slukvin
Specification of endothelial cells (ECs) into arterial, venous, and lymphatic cells is a crucial process of vascular development, and expanding our knowledge about EC specification from human pluripotent stem cells (hPSCs) will aid the design of optimal strategies for producing desired types of ECs for therapies. In our prior studies, we revealed that hPSC-derived VE-cadherin(V)+ CD31+ CD34+ ECs are heterogeneous and include at least three major subsets with distinct hemogenic properties: V+ CD43/235a- CD73- hemogenic endothelial progenitors (HEPs), V+ CD43lo CD235a+ 73- angiogenic hematopoietic progenitors (AHPs), and V+ CD43/235a- 73+ non-HEPs...
March 27, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29568483/transplantation-of-endothelial-progenitor-cells-attenuated-paraquat-induced-acute-lung-injury-via-mir-141-3p-notch-nrf2-axis
#15
Yan Jin, Wei Liu, Xiaowei Liu, Tao Ma, Chen Yang, Quan Cai, Zhi Liu
Background: Paraquat (PQ) presents with high toxicity for humans and animals, and the lungs become the main target organ by the poisoning of PQ leading to acute lung injury. Endothelial progenitor cells (EPCs) were proved to have the repair function on acute lung injury (ALI). We aimed to invatigate the underlying mechanism of EPCs in PQ-induced ALI involving miR-141-3p. Methods: Endothelial progenitor cells were isolated from peripheral blood of C57BL/6J mice and identified by flow cytometry...
2018: Cell & Bioscience
https://www.readbyqxmd.com/read/29566124/sustained-release-of-endothelial-progenitor-cell-derived-extracellular-vesicles-from-shear-thinning-hydrogels-improves-angiogenesis-and-promotes-function-after-myocardial-infarction
#16
Carol W Chen, Leo L Wang, Samir Zaman, Jon Gordon, Maria F Arisi, Chantel M Venkataraman, Jennifer J Chung, George Hung, Ann C Gaffey, Lynn A Spruce, Hossein Fazelinia, Robert C Gorman, Steven H Seeholzer, Jason A Burdick, Pavan Atluri
Aims: Previous studies have demonstrated improved cardiac function following myocardial infarction (MI) after administration of endothelial progenitor cells (EPCs) into ischemic myocardium. A growing body of literature supports paracrine effectors, including extracellular vesicles (EVs), as the main mediators of the therapeutic benefits of EPCs. The use of paracrine factors is an attractive strategy that harnesses the effects of cell therapy without concerns of cell engraftment or viability...
March 16, 2018: Cardiovascular Research
https://www.readbyqxmd.com/read/29564766/isolation-and-culture-of-mouse-placental-endothelial-cells
#17
Lijun Chi, Paul Delgado-Olguin
Isolation and culture of endothelial cells (ECs) is a useful tool to study the cellular processes involved in vascular development and vascular maturation. In this chapter, we describe a method to isolate and culture endothelial cells from placentae. This method takes advantage of two transgenes: ROSA26mT/mG , which drives the expression of GFP upon Cre-mediated recombination, and Tie2-Cre, which expresses Cre driven by the Tie2 promoter in endothelial progenitors and their descendants. GFP-expressing endothelial cells are isolated through fluorescence-activated cell sorting (FACS)...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29556525/mobilization-of-progenitor-cells-and-assessment-of-vessel-healing-after-second-generation-drug-eluting-stenting-by-optical-coherence-tomography
#18
Masashi Sakuma, Takahisa Nasuno, Shichiro Abe, Syotaro Obi, Shigeru Toyoda, Isao Taguchi, Ryoichi Sohma, Ken-Ichi Inoue, Setsu Nishino, Koichi Node, Guiherme Attizzani, Hiram Bezerra, Marco Costa, Daniel Simon, Teruo Inoue
Background: Bone marrow-derived progenitor cells likely contribute to both endothelial- and smooth muscle cell-dependent healing responses in stent-injured vessel sites. This study aimed to assess mobilization of progenitor cells and vessel healing after zotarolimus-eluting (ZES) and everolimus-eluting (EES) stents. Methods and results: In 63 patients undergoing coronary stent implantation, we measured circulating CD34 + CD133 + CD45low cells and serum levels of biomarkers relevant to stem cell mobilization...
March 2018: IJC Heart & Vasculature
https://www.readbyqxmd.com/read/29555374/exosomes-as-emerging-players-in-cancer-biology
#19
Nuno Couto, Sergio Caja, Joana Maia, Maria Carolina Strano Moraes, Bruno Costa-Silva
Oncologic diseases do not behave as isolated entities. Instead, they are based on complex systemic networks involving cell-cell communication between cancerous and healthy cells of the host, which may either facilitate or prevent cancer progression. In addition to cell-cell contacts, cells communicate through secreted factors in a process modulated by ligand concentration, receptor availability and synergy amongst several signaling circuits. Of these secreted factors, exosomes, 30-150 nm membrane vesicles of endocytic origin released by virtually all cells, have emerged as important cell-cell communication players both in physiological and pathological scenarios by being carriers of all the main biomolecules, including lipids, proteins, DNAs, messenger RNAs and microRNA, and performing intercellular transfer of components, locally and systemically...
March 16, 2018: Biochimie
https://www.readbyqxmd.com/read/29550796/in-vitro-model-of-vasculo-angiogenesis-demonstration-that-bone-marrow-derived-endothelial-progenitor-cells-form-new-hybrid-capillary-blood-vessels-jointly-with-gastric-endothelial-cells
#20
A Ahluwalia, M K Jones, I Brzozowska, A S Tarnawski
Regeneration of blood vessels (neovascularization) is critical for tissue injury healing. The contribution of bone marrow-derived endothelial progenitor cells (BMD-EPCs) to neovascularization during tissue injury healing is not fully elucidated and it is not clear whether BMD-EPCs can form new capillary blood vessels independently or jointly with fully differentiated endothelial cells (ECs). The aim of this study was to establish an in vitro model of vasculogenesis/angiogenesis by co-culture of BMD-EPCs and gastric endothelial cells (GECs) on Matrigel, examine direct interactions of these cells; and, identify the mechanisms involved...
December 2017: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
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