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https://www.readbyqxmd.com/read/29471788/modelling-physiological-and-pathological-conditions-to-study-pericyte-biology-in-brain-function-and-dysfunction
#1
Justin Rustenhoven, Leon C Smyth, Deidre Jansson, Patrick Schweder, Miranda Aalderink, Emma L Scotter, Edward W Mee, Richard L M Faull, Thomas I-H Park, Mike Dragunow
BACKGROUND: Brain pericytes ensheathe the endothelium and contribute to formation and maintenance of the blood-brain-barrier. Additionally, pericytes are involved in several aspects of the CNS immune response including scarring, adhesion molecule expression, chemokine secretion, and phagocytosis. In vitro cultures are routinely used to investigate these functions of brain pericytes, however, these are highly plastic cells and can display differing phenotypes and functional responses depending on their culture conditions...
February 22, 2018: BMC Neuroscience
https://www.readbyqxmd.com/read/29469037/hepatitis-e-virus-genotype-1-cases-imported-to-portugal-from-india-2016
#2
João R Mesquita, Madalena Almeida-Santos, Maria Fernandes, Fernando Maltez, Sara Lino, Liliana Alves, Joana Abreu-Silva, Ricardo M S Oliveira, Martin D Curran, Maria S J Nascimento
Hepatitis E in industrialized countries is mainly associated with genotype 3 hepatitis E virus (HEV) and normally causes a sporadic self-limiting disease in immunocompetent individuals. Unlike genotype 3, genotypes 1 and 2 circulate in developing countries, produce severe disease and occur in the epidemic form. Hepatitis E occurring in travellers returning from endemic areas in developing countries is not a novel epidemiological occurrence, however the vast majority of cases remain to be genetically studied...
March 1, 2018: Annals of Hepatology
https://www.readbyqxmd.com/read/29467896/17%C3%AE-estradiol-regulates-the-malignancy-of-cancer-stem-like-cells-derived-from-the-mcf7-cell-line-partially-through-sox2
#3
Lian Guo, Feng Li, Mengyuan Wang, Yue Xu, Bangqiong Wang, Dongchuan Ran, Xianxiang Zhang
As a major common malignant tumor in women, the malignant behavior of breast cancer, which includes tumorigenesis and metastasis, is associated with estrogen, particularly 17β-estradiol (E2). With accumulating evidence demonstrating that cancer stem-like cells (CSCs) serve a function in the malignant behavior of breast cancer, including metastasis, recurrence and chemoresistance, the effects of E2 on the physiological processes of CSCs have been attracting more attention. In the present study, in order to investigate the effects of E2 on CSCs, CSCs from the MCF7 breast cancer cell line were isolated and treated with 1, 10 and 50 nM E2...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29467020/insulin-like-growth-factor-1-mediated-regulation-of-mir-193a-expression-promotes-the-migration-and-proliferation-of-c-kit-positive-mouse-cardiac-stem-cells
#4
Yuning Sun, Rongfeng Xu, Jia Huang, Yuyu Yao, Xiaodong Pan, Zhongpu Chen, Genshan Ma
BACKGROUND: C-kit-positive cardiac stem cells (CSCs) have been shown to be a promising candidate treatment for myocardial infarction and heart failure. Insulin-like growth factor (IGF)-1 is an anabolic growth hormone that regulates cellular proliferation, differentiation, senescence, and death in various tissues. Although IGF-1 promotes the migration and proliferation of c-kit-positive mouse CSCs, the underlying mechanism remains unclear. METHODS: Cells were isolated from adult mouse hearts, and c-kit-positive CSCs were separated using magnetic beads...
February 21, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29465808/investigating-the-potential-barrier-function-of-nanostructured-materials-formed-in-engineered-barrier-systems-ebs-designed-for-nuclear-waste-isolation
#5
REVIEW
Jaime Cuevas, Ana Isabel Ruiz, Raúl Fernández
Clay and cement are known nano-colloids originating from natural processes or traditional materials technology. Currently, they are used together as part of the engineered barrier system (EBS) to isolate high-level nuclear waste (HLW) metallic containers in deep geological repositories (DGR). The EBS should prevent radionuclide (RN) migration into the biosphere until the canisters fail, which is not expected for approximately 103 years. The interactions of cementitious materials with bentonite swelling clay have been the scope of our research team at the Autonomous University of Madrid (UAM) with participation in several European Union (EU) projects from 1998 up to now...
February 21, 2018: Chemical Record: An Official Publication of the Chemical Society of Japan ... [et Al.]
https://www.readbyqxmd.com/read/29463289/strain-dependent-effects-of-clinical-echovirus-30-outbreak-isolates-at-the-blood-csf-barrier
#6
Tobias Dahm, Ortwin Adams, Sindy Boettcher, Sabine Diedrich, Vasily Morozov, Grant Hansman, Petra Fallier-Becker, Sebastian Schädler, Claus J Burkhardt, Christel Weiss, Carolin Stump-Guthier, Hiroshi Ishikawa, Horst Schroten, Christian Schwerk, Tobias Tenenbaum, Henriette Rudolph
BACKGROUND: Echovirus (E) 30 (E-30) meningitis is characterized by neuroinflammation involving immune cell pleocytosis at the protective barriers of the central nervous system (CNS). In this context, infection of the blood-cerebrospinal fluid barrier (BCSFB), which has been demonstrated to be involved in enteroviral CNS pathogenesis, may affect the tight junction (TJ) and adherens junction (AJ) function and morphology. METHODS: We used an in vitro human choroid plexus epithelial (HIBCPP) cell model to investigate the effect of three clinical outbreak strains (13-311, 13-759, and 14-397) isolated in Germany in 2013, and compared them to E-30 Bastianni...
February 20, 2018: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29459236/toward-rapid-analysis-forecast-and-discovery-of-bioactive-compounds-from-herbs-by-jointly-using-thin-layer-chromatography-and-ratiometric-surface-enhanced-raman-spectroscopy-technique
#7
Xiaoling Gu, Yang Jin, Fang Dong, Yueqing Cai, Zhengyi You, Junhui You, Liying Zhang, Shuhu Du
Conventional isolation and identification of active compounds from herbs have been extensively reported by using various chromatographic and spectroscopic techniques. However, how to quickly discover new bioactive ingredients from natural sources still remains a challenging task due to the interference of their similar structures or matrices. Here, we present a grand approach for rapid analysis, forecast and discovery of bioactive compounds from herbs based on a hyphenated strategy of thin layer chromatography and ratiometric surface-enhanced Raman spectroscopy...
February 11, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29458855/streptomyces-puniceus-strain-as13-production-characterization-and-evaluation-of-bioactive-metabolites-a-new-face-of-dinactin-as-an-antitumor-antibiotic
#8
Aehtesham Hussain, Muzafar Ahmad Rather, Mohd Saleem Dar, N A Dangroo, Mushtaq A Aga, Arem Qayum, Aabid Manzoor Shah, Zahoor Ahmad, Mohd Jamal Dar, Qazi Parvaiz Hassan
A highly active actinobacterial strain isolated from untapped areas of Northwestern Himalayas and characterised as Streptomyces puniceus strain AS13 by 16S rRNA gene sequencing was selected for production of bioactive metabolites. The bioassay-guided fractionation of microbial cultured ethyl acetate extract of the strain, led to isolation of macrotetrolide compound 1 (Dinactin) and compound 2 (1-(2,4-dihydroxy-6-methylphenyl)-ethanone). Structures of the isolated compounds were elucidated interpretation of NMR and other spectroscopic data including HR-ESI-MS, FT-IR...
March 2018: Microbiological Research
https://www.readbyqxmd.com/read/29455737/foreclosure-migration-and-neighborhood-outcomes-moving-toward-segregation-and-disadvantage
#9
Matthew Hall, Kyle Crowder, Amy Spring, Ryan Gabriel
The US housing crisis during the late 2000s was arguably the most devastating residential disaster of the last century, sending millions of families into foreclosure and destroying billions in household wealth. An understudied aspect of the crisis was the spike in local migration that followed the foreclosure surge. In this paper, we assess the residential consequences of these moves, by exploring how foreclosure-induced migration affected the racial and socioeconomic composition of affected families' neighborhoods...
February 2018: Social Science Research
https://www.readbyqxmd.com/read/29453733/egfp-transgene-a-useful-tool-to-track-transplanted-bone-marrow-mononuclear-cell-contribution-to-peripheral-remyelination
#10
Gonzalo Piñero, Vanina Usach, Paula A Soto, Paula V Monje, Patricia Setton-Avruj
Bone marrow mononuclear cells (BMMC) constitute a heterogeneous population with potential to promote tissue regeneration. For this reason, this cell fraction has recently become a therapeutic alternative to mesenchymal stem cells, as culture is not required and phenotypic transformations can be hence avoided. In this work, and in order to attain long-lasting cell labeling and study longer survival times, we used BMMC isolated from adult transgenic rats expressing GFP to reproduce our wild type model and evaluate their remyelination ability in a reversible model of Wallerian degeneration...
February 16, 2018: Transgenic Research
https://www.readbyqxmd.com/read/29453401/endothelial-smoothened-dependent-hedgehog-signaling-is-not-required-for-sonic-hedgehog-induced-angiogenesis-or-ischemic-tissue-repair
#11
Rajesh Gupta, Alexander R Mackie, Sol Misener, Lijun Liu, Douglas W Losordo, Raj Kishore
Sonic Hedgehog (Shh) signaling induces neovascularization and angiogenesis. It is not known whether the hedgehog signaling pathway in endothelial cells is essential to angiogenesis. Smoothened (Smo) transduces hedgehog signaling across the cell membrane. This study assessed whether endothelial Smoothened-dependent Shh signaling is required for Shh-mediated angiogenesis and ischemic tissue repair. Endothelial-specific smoothened knockout mice, eSmo Null were created using Cre-lox recombination system. eSmo Null mice had no observable phenotype at baseline and showed normal cardiac function...
February 16, 2018: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29452896/do-subscapularis-tears-really-result-in-superior-humeral-migration
#12
Mehmet Cetinkaya, Muhammet Baybars Ataoglu, Mustafa Ozer, Tacettin Ayanoglu, Ali Yusuf Oner, Ulunay Kanatli
OBJECTIVES: The aim of this study was to analyse the effect of subscapularis tear on superior humeral excursion (SHE) and acromiohumeral distance (AHD). The hypothesis was that subscapularis tears do not result in superior humeral excursion. METHODS: Patients who underwent shoulder arthroscopy between August of 2011 and 2015 were reevaluated. Those with isolated Bankart lesion were used as control group and included in the Group 1, isolated full-thickness supraspinatus tear in the Group 2, isolated subscapularis tear in the Group 3, and combined subscapularis and supraspinatus tear in the Group 4...
February 13, 2018: Acta Orthopaedica et Traumatologica Turcica
https://www.readbyqxmd.com/read/29452840/borrelidins-f-i-cytotoxic-and-cell-migration-inhibiting-agents-from-mangrove-derived-streptomyces-rochei-scsio-zj89
#13
Jianbin Sun, Junli Shao, Changli Sun, Yongxiang Song, Qinglian Li, Laichun Lu, Yunfeng Hu, Chun Gui, Hua Zhang, Jianhua Ju
Borrelidin A (1) is produced by several species of Streptomyces and within its bioactive scaffold, the vinylic nitrile moiety is essential for activity. We report herein newly discovered members of the borrelidin family, borrelidin F (2), borrelidin G (3), borrelidin H (4) and borrelidin I (5); all were isolated from Streptomyces rochei SCSIO ZJ89 originating from a mangrove-derived sediment sample. These structurally diverse metabolites enabled a number of new structure-activity relationships (SARs) to be identified, especially with respect to the different configurations at the C11-OH and C12-C15 double bonds for which the absolute configurations were determined using spectroscopic methods...
February 7, 2018: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29450586/the-host-control-of-a-clinical-isolate-strain-of-p-aeruginosa-infection-is-independent-of-nod-1-but-depends-on-myd88
#14
Fabiane Sônego, Fernanda V S Castanheira, Catarina V Horta, Alexandre Kanashiro, Paula G Czaikoski, Dario S Zamboni, José Carlos Alves-Filho, Fernando Q Cunha
OBJECTIVE AND DESIGN: The objective of this study was to investigate the role of Nod1 in the recruitment of neutrophils into the infection site and in the establishment of the inflammatory response elicited by a clinical isolate strain of P. aeruginosa in vivo, while comparing it to the well-established role of MyD88 in this process. SUBJECTS: Wild-type, Nod1 -/- and MyD88 -/- mice, all with a C57Bl/6 background. METHODS: Mice were intranasally infected with Pseudomonas aeruginosa DZ605...
February 15, 2018: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
https://www.readbyqxmd.com/read/29449707/modulation-of-cardiac-stem-cell-characteristics-by-metoprolol-in-hypertensive-heart-disease
#15
Sherin Saheera, Ajay Godwin Potnuri, Renuka R Nair
Cardiac stem cells (CSCs) play a vital role in cardiac remodeling. Uncontrolled hypertension leads to cardiac hypertrophy, followed by cardiac failure. Pathological remodeling is associated with enhanced oxidative stress. Decreased cardiac stem cell efficiency is speculated in heart diseases. Maintaining a healthy stem cell population is essential for preventing progressive cardiac remodeling. Some anti-hypertensive drugs are cardioprotective. However, the effect of these drugs on CSCs has not been investigated...
February 15, 2018: Hypertension Research: Official Journal of the Japanese Society of Hypertension
https://www.readbyqxmd.com/read/29448134/enhancing-proliferation-and-optimizing-the-culture-condition-for-human-bone-marrow-stromal-cells-using-hypoxia-and-fibroblast-growth-factor-2
#16
Jung-Seok Lee, Seul Ki Kim, Byung-Joo Jung, Seong-Bok Choi, Eun-Young Choi, Chang-Sung Kim
This study aimed to determine the cellular characteristics and behaviors of human bone marrow stromal cells (hBMSCs) expanded in media in a hypoxic or normoxic condition and with or without fibroblast growth factor-2 (FGF-2) treatment. hBMSCs isolated from the vertebral body and expanded in these four groups were evaluated for cellular proliferation/migration, colony-forming units, cell-surface characterization, in vitro differentiation, in vivo transplantation, and gene expression. Culturing hBMSCs using a particular environmental factor (hypoxia) and with the addition of FGF-2 increased the cellular proliferation rate while enhancing the regenerative potential, modulated the multipotency-related processes (enhanced chondrogenesis-related processes/osteogenesis, but reduced adipogenesis), and increased cellular migration and collagen formation...
January 9, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29444110/leukemia-inhibitory-factor-produced-by-fibroblasts-within-tumor-stroma-participates-in-invasion-of-oral-squamous-cell-carcinoma
#17
Yae Ohata, Maiko Tsuchiya, Hideaki Hirai, Satoshi Yamaguchi, Takumi Akashi, Kei Sakamoto, Akira Yamaguchi, Tohru Ikeda, Kou Kayamori
The interaction between cancer cells and the cancer stroma plays a crucial role in tumor progression and metastasis in diverse malignancies, including oral cancer. However, the mechanism underlying this interaction remains incompletely elucidated. Here, to investigate the interaction between oral cancer cells and fibroblasts, which are major cellular components of the tumor stroma, we conducted an in vitro study by using human oral squamous cell carcinoma (OSCC) cell lines and normal human dermal fibroblasts (NHDFs)...
2018: PloS One
https://www.readbyqxmd.com/read/29440653/genetic-and-morphological-support-for-possible-sympatric-origin-of-fish-from-subterranean-habitats
#18
Iraj Hashemzadeh Segherloo, Eric Normandeau, Laura Benestan, Clément Rougeux, Guillaume Coté, Jean-Sébastien Moore, NabiAllah Ghaedrahmati, Asghar Abdoli, Louis Bernatchez
Two blind Iran cave barbs, Garra typhlops and Garra lorestanensis, exist in sympatry in a single subterranean habitat, raising the hypothesis that they may represent a case of sympatric speciation following a colonization event. Their different mental disc forms have prompted some authors to propose the alternative hypothesis of two separate colonization events. In this study, we analysed a genome-wide panel of 11,257 SNPs genotyped by means of genotyping-by-sequencing combined with mitochondrial cytochrome c oxidase sub-unit I sequence data, field observations and morphological traits to test these two hypotheses...
February 13, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29437500/use-of-the-sphenoid-flap-in-repair-of-the-wide-cleft-palate
#19
Khurram Khan, Joseph Hardwicke, Rimante Seselgyte, Paul Morris, Guy Thorburn, Loshan Kangesu, Marc C Swan, Bruce Richard
OBJECTIVE: In wide palatal defects, closure of the nasal layer can prove a considerable challenge. Mobilizing nasal flaps posteriorly usually facilitates soft palate closure. However, the defect is often too wide within the hard palate; hence, bilateral vomerine flaps are frequently required. Despite this, there is often a small defect in the nasal layer at the posterior septum (typically equating to the hard-soft palate junction), which has to be left to heal by secondary intention with the resulting increased risk of fistula formation and the potential deleterious long-term effect on speech due to cicatricial migration of the reconstructed levator sling anteriorly...
March 2018: Cleft Palate-craniofacial Journal
https://www.readbyqxmd.com/read/29436831/on-the-formation-of-c-2v-c-72-11188-cl-4-a-particularly-stable-non-ipr-fullerene
#20
Antonio Moreno-Vicente, Laura Abella, Khalid Azmani, Antonio Rodríguez-Fortea, Josep M Poblet
Halogenation has been one of the most used strategies to explore the reactivity of empty carbon cages. In particular, the higher reactivity of non-IPR fullerenes, i.e. those fullerenes that do not satisfy the Isolated Pentagon Rule (IPR), has been used to functionalize and capture these less stable fullerenes. Here, we have explored the stability and reactivity of the non-IPR isomer C72(11188) with C2v symmetry, which is topologically linked to the only IPR isomer of C70. DFT calculations and Car-Parrinello molecular dynamics simulations suggest that chlorination takes places initially in non-specific sites, once carbon cages are formed...
February 13, 2018: Journal of Physical Chemistry. A
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