keyword
MENU ▼
Read by QxMD icon Read
search

isolation with migration

keyword
https://www.readbyqxmd.com/read/28078190/loss-of-quiescence-and-self-renewal-capacity-of-hematopoietic-stem-cell-in-an-in-vitro-leukemic-niche
#1
Natalia-Del Pilar Vanegas, Jean-Paul Vernot
BACKGROUND: Leukemic and mesenchymal stem cells interact in the leukemic microenvironment and affect each other differently. This interplay has also important implications for the hematopoietic stem cell (HSC) biology and function. This study evaluated human HSC self-renewal potential and quiescence in an in vitro leukemic niche without leukemic cells. METHODS: A leukemic niche was established by co-culturing mesenchymal stem cells with a fresh conditioned medium obtained from a leukemic (REH) cell line...
2017: Experimental Hematology & Oncology
https://www.readbyqxmd.com/read/28074844/no-signs-of-inbreeding-despite-long-term-isolation-and-habitat-fragmentation-in-the-critically-endangered-montseny-brook-newt-calotriton-arnoldi
#2
E Valbuena-Ureña, A Soler-Membrives, S Steinfartz, P Orozco-terWengel, S Carranza
Endemic species with restricted geographic ranges potentially suffer the highest risk of extinction. If these species are further fragmented into genetically isolated subpopulations, the risk of extinction is elevated. Habitat fragmentation is generally considered to have negative effects on species survival, despite some evidence for neutral or even positive effects. Typically, non-negative effects are ignored by conservation biology. The Montseny brook newt (Calotriton arnoldi) has one of the smallest distribution ranges of any European amphibian (8 km(2)) and is considered critically endangered by the International Union for Conservation of Nature...
January 11, 2017: Heredity
https://www.readbyqxmd.com/read/28071738/purification-and-differentiation-of-human-adipose-derived-stem-cells-by-membrane-filtration-and-membrane-migration-methods
#3
Hong Reng Lin, Chao-Wen Heish, Cheng-Hui Liu, Saradaprasan Muduli, Hsing-Fen Li, Akon Higuchi, S Suresh Kumar, Abdullah A Alarfaj, Murugan A Munusamy, Shih-Tien Hsu, Da-Chung Chen, Giovanni Benelli, Kadarkarai Murugan, Nai-Chen Cheng, Han-Chow Wang, Gwo-Jang Wu
Human adipose-derived stem cells (hADSCs) are easily isolated from fat tissue without ethical concerns, but differ in purity, pluripotency, differentiation ability, and stem cell marker expression, depending on the isolation method. We isolated hADSCs from a primary fat tissue solution using: (1) conventional culture, (2) a membrane filtration method, (3) a membrane migration method where the primary cell solution was permeated through membranes, adhered hADSCs were cultured, and hADSCs migrated out from the membranes...
January 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28071612/expression-of-raf-kinase-inhibitor-protein-in-human-hepatoma-tissues-by-two-dimensional-gel-electrophoresis-and-matrix-assisted-laser-desorption-ionization-time-of-flight-methods
#4
D A Tsao, Y F Shiau, C S Tseng, H R Chang
PURPOSE: Hepatocellular carcinoma (HCC) is the most common malignant liver tumor. To reduce the mortality and improve the effectiveness of therapy, it is important to search for changes in tumor-specific biomarkers whose function may involve in disease progression and which may be useful as potential therapeutic targets. Materials and Mehtods: In this study, we use two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to observe proteome alterations of 12 tissue pairs isolated from HCC patients: Normal and tumorous tissue...
April 2016: Indian Journal of Cancer
https://www.readbyqxmd.com/read/28070968/bacterial-short-chain-fatty-acid-metabolites-modulate-the-inflammatory-response-against-infectious-bacteria
#5
R O Corrêa, A Vieira, E M Sernaglia, M Lancellotti, A T Vieira, M J Avila-Campos, H G Rodrigues, M A R Vinolo
Short chain fatty acids (SCFAs), predominately acetic, propionic and butyric acids, are bacterial metabolites with an important role in the maintenance of homeostasis due to their metabolic and immunomodulatory actions. Some evidence suggests that they may also be relevant during infections. Therefore, we aimed to investigate the effects of SCFAs in the effector functions of neutrophils to an opportunistic pathogenic bacteria, Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans). Using a subcutaneous model to generate a mono, isolated infection of A...
January 9, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28068953/pregnant-human-peripheral-leukocyte-migration-during-several-late-pregnancy-clinical-conditions-a-cross-sectional-observational-study
#6
Jun Takeda, Xin Fang, David M Olson
BACKGROUND: Parturition at term and preterm is characterized by sterile inflammatory processes occurring in the absence of infection whereby peripheral leukocytes infiltrate gestational tissues in response to chemotactic signals. In response to a homing signal, recruited leukocytes undergo diapedesis and extravasate through capillaries, migrating into stromal tissue. There they interact with resident immune and stromal cells to produce a mixture of matrix metalloproteinases, prostaglandins and cytokines including interleukin-1β (IL-1β) and IL-6 that in turn transform the uterus from pregnancy to parturition...
January 10, 2017: BMC Pregnancy and Childbirth
https://www.readbyqxmd.com/read/28060841/genomes-of-fasciola-hepatica-from-the-americas-reveal-colonization-with-neorickettsia-endobacteria-related-to-the-agents-of-potomac-horse-and-human-sennetsu-fevers
#7
Samantha N McNulty, Jose F Tort, Gabriel Rinaldi, Kerstin Fischer, Bruce A Rosa, Pablo Smircich, Santiago Fontenla, Young-Jun Choi, Rahul Tyagi, Kymberlie Hallsworth-Pepin, Victoria H Mann, Lakshmi Kammili, Patricia S Latham, Nicolas Dell'Oca, Fernanda Dominguez, Carlos Carmona, Peter U Fischer, Paul J Brindley, Makedonka Mitreva
Food borne trematodes (FBTs) are an assemblage of platyhelminth parasites transmitted through the food chain, four of which are recognized as neglected tropical diseases (NTDs). Fascioliasis stands out among the other NTDs due to its broad and significant impact on both human and animal health, as Fasciola sp., are also considered major pathogens of domestic ruminants. Here we present a reference genome sequence of the common liver fluke, Fasciola hepatica isolated from sheep, complementing previously reported isolate from cattle...
January 6, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28060837/beneficial-effects-of-reconstituted-high-density-lipoprotein-rhdl-on-circulating-cd34-cells-in-patients-after-an-acute-coronary-syndrome
#8
Catherine Gebhard, Eric Rhéaume, Colin Berry, Geneviève Brand, Anne-Elen Kernaleguen, Gabriel Théberge-Julien, Mohammad Afaque Alam, Candace Y W Lee, Laurianne Boileau, Malorie Chabot-Blanchet, Marie-Claude Guertin, Marc-André Lavoie, Jean Grégoire, Réda Ibrahim, Philippe L'Allier, Jean-Claude Tardif
BACKGROUND: High-density lipoproteins (HDL) favorably affect endothelial progenitor cells (EPC). Circulating progenitor cell level and function are impaired in patients with acute coronary syndrome (ACS). This study investigates the short-term effects of reconstituted HDL (rHDL) on circulating progenitor cells in patients with ACS. METHODS AND FINDINGS: The study population consisted of 33 patients with recent ACS: 20 patients from the ERASE trial (randomized to receive 4 weekly intravenous infusions of CSL-111 40 mg/kg or placebo) and 13 additional patients recruited as controls using the same enrolment criteria...
2017: PloS One
https://www.readbyqxmd.com/read/28060811/stromal-fibroblasts-from-the-interface-zone-of-triple-negative-breast-carcinomas-induced-epithelial-mesenchymal-transition-and-its-inhibition-by-emodin
#9
Hsiang-Chi Hsu, Liang-Chih Liu, Hao-Yu Wang, Chao-Ming Hung, Ying-Chao Lin, Chi-Tang Ho, Tzong-Der Way
"Triple negative breast cancer" (TNBC) is associated with a higher rate and earlier time of recurrence and worse prognosis after recurrence. In this study, we aimed to examine the crosstalk between fibroblasts and TNBC cells. The fibroblasts were isolated from TNBC patients' tissue in tumor burden zones, distal normal zones and interface zones. The fibroblasts were indicated as cancer-associated fibroblasts (CAFs), normal zone fibroblasts (NFs) and interface zone fibroblasts (INFs). Our study found that INFs grew significantly faster than NFs and CAFs in vitro...
2017: PloS One
https://www.readbyqxmd.com/read/28060794/postnatal-human-enteric-neuronal-progenitors-can-migrate-differentiate-and-proliferate-in-embryonic-and-postnatal-aganglionic-gut-environments
#10
Lily S Cheng, Ryo Hotta, Hannah K Graham, Jaime Belkind-Gerson, Nandor Nagy, Allan M Goldstein
BACKGROUND: Enteric neural stem/progenitor cells (ENSCs) offer an innovative approach to treating Hirschsprung disease (HSCR) and other enteric neuropathies. However, postnatal-derived human ENSCs have not been thoroughly characterized and their behavior in the embryonic and postnatal intestinal environment is unknown. METHODS: ENSCs were isolated from the intestines of 25 patients undergoing bowel resection, including 7 children with HSCR. Neuronal differentiation and proliferation of ENSCs from submucosal and myenteric plexuses from patients with and without HSCR were characterized...
January 6, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28060112/spontaneous-fracture-and-migration-of-a-totally-implanted-port-device-to-pulmonary-artery-in-acute-leukemia-child
#11
Olfa Kassar, Rania Hammemi, Mahdi Ben Dhaou, Samir Kammoun, Moez Elloumi
Totally implanted port devices are important for the administration of fluid and chemotherapeutic agents. However, their use may be associated with serious complications, such as catheter fracture and embolism. Most data on port catheter embolization consist of isolated case reports. We report a 6-year-old boy with relapse of an acute lymphoblastic leukemia. He presented with dysfunction of his totally implanted port device. Radiologic examination revealed a catheter fracture. Echocardiography showed the catheter embolized into the right ventricle...
January 5, 2017: Journal of Pediatric Hematology/oncology
https://www.readbyqxmd.com/read/28056072/clarifying-the-cryptic-host-specificity-of-blastocystis-spp-isolates-from-alouatta-palliata-and-a-pigra-howler-monkeys
#12
Claudia Villanueva-Garcia, Elias Jose Gordillo-Chavez, Eduardo Lopez-Escamilla, Emilio Rendon-Franco, Claudia Irais Muñoz-Garcia, Lilia Gama, Williams Arony Martinez-Flores, Nayeli Gonzalez-Rodriguez, Mirza Romero-Valdovinos, Hilda Diaz-Lopez, Jose Galian, Guiehdani Villalobos, Pablo Maravilla, Fernando Martinez-Hernandez
Although the presence of cryptic host specificity has been documented in Blastocystis, differences in infection rates and high genetic polymorphism within and between populations of some subtypes (ST) have impeded the clarification of the generalist or specialist specificity of this parasite. We assessed the genetic variability and host specificity of Blastocystis spp. in wild howler monkeys from two rainforest areas in the southeastern region of Mexico. Fecal samples of 225 Alouatta palliata (59) and A. pigra (166) monkeys, belonging to 16 sylvatic sites, were analyzed for infection with Blastocystis ST using a region of the small subunit rDNA (SSUrDNA) gene as a marker...
2017: PloS One
https://www.readbyqxmd.com/read/28053879/lysozyme-association-with-circulating-rna-extracellular-vesicles-and-chronic-stress
#13
Sarah K Abey, Yuana Yuana, Paule V Joseph, Natnael D Kenea, Nicolaas H Fourie, LeeAnne B Sherwin, Gregory E Gonye, Paul A Smyser, Erin S Stempinski, Christina M Boulineaux, Kristen R Weaver, Christopher K E Bleck, Wendy A Henderson
BACKGROUND: Stress has demonstrated effects on inflammation though underlying cell-cell communication mechanisms remain unclear. We hypothesize that circulating RNAs and extracellular vesicles (EVs) in patients with chronic stress contain signals with functional roles in cell repair. METHODS: Blood transcriptome from patients with Irritable Bowel Syndrome versus controls were compared to identify signaling pathways and effectors. Plasma EVs were isolated (size-exclusion chromatography) and characterized for effectors' presence (immunogold labelling-electron microscopy)...
June 2017: BBA Clinical
https://www.readbyqxmd.com/read/28049708/modeling-human-population-separation-history-using-physically-phased-genomes
#14
Shiya Song, Elzbieta Sliwerska, Sarah Emery, Jeffrey M Kidd
Phased haplotype sequences are a key component in many population genetic analyses since variation in haplotypes reflects the action of recombination, selection, and changes in population size. In humans, haplotypes are typically estimated from unphased sequence or genotyping data using statistical models applied to large reference panels. To assess the importance of correct haplotype phase on population history inference, we performed fosmid pool sequencing and resolved phased haplotypes of five individuals from diverse African populations (including Yoruba, Esan, Gambia, Maasai, and Mende)...
January 2017: Genetics
https://www.readbyqxmd.com/read/28043811/honokiol-induces-proteasomal-degradation-of-aml1-eto-oncoprotein-via-increasing-ubiquitin-conjugase-ubch8-expression-in-leukemia
#15
Bin Zhou, Haiying Li, Chongyun Xing, Haige Ye, Jianhua Feng, Jianbo Wu, Zhongqiu Lu, Jing Fang, Shenmeng Gao
AML1-ETO is the most common oncoprotein leading to acute myeloid leukemia (AML), in which 5-year survival rate is only about 30%. However, currently there are no specific therapies for AML patients with AML1-ETO. Here, we report that AML1-ETO protein is rapidly degraded by Honokiol (HNK), a natural phenolic compound isolated from the plant Magnolia officinalis. HNK induced the degradation of AML1-ETO in a concentration- and time-dependent manner in leukemic cell lines and primary AML blasts with t(8;21) translocation...
December 30, 2016: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28042326/exosomes-derived-from-mir-140-5p-overexpressing-human-synovial-mesenchymal-stem-cells-enhance-cartilage-tissue-regeneration-and-prevent-osteoarthritis-of-the-knee-in-a-rat-model
#16
Shi-Cong Tao, Ting Yuan, Yue-Lei Zhang, Wen-Jing Yin, Shang-Chun Guo, Chang-Qing Zhang
OBJECTIVES: Osteoarthritis (OA) is the most common joint disease throughout the world. Exosomes derived from miR-140-5p-overexpressing synovial mesenchymal stem cells (SMSC-140s) may be effective in treating OA. We hypothesized that exosomes derived from SMSC-140 (SMSC-140-Exos) would enhance the proliferation and migration abilities of articular chondrocytes (ACs) without harming extracellular matrix (ECM) secretion. METHODS: SMSCs were transfected with or without miR-140-5p...
2017: Theranostics
https://www.readbyqxmd.com/read/28039603/place-matters-in-non-traditional-migration-areas-exploring-barriers-to-healthcare-for-latino-immigrants-by-region-neighborhood-and-community-health-center
#17
Michael Topmiller, Jenny Zhen-Duan, Farrah J Jacquez, Lisa M Vaughn
This paper identifies differences in adult Latino immigrant barriers to healthcare in the Cincinnati area in Hamilton County, OH, on three levels: by region, by neighborhood, and by community health center. Secondary data analysis was performed on 439 surveys. Respondents were aggregated by the geographic regions and neighborhoods where they live and by two community health centers where they receive care. Outcome measures included pragmatic and skill barrier indices adapted from the Barriers to Care Questionnaire (BCQ); the pragmatics index consists logistical barriers, including transportation and cost; the skills index is made up of items related to navigating the healthcare system, including communicating with physicians and completing paperwork...
December 30, 2016: Journal of Racial and Ethnic Health Disparities
https://www.readbyqxmd.com/read/28039430/inflammation-and-vascular-smooth-muscle-cell-dedifferentiation-following-carotid-artery-ligation
#18
Brian Paul Herring, April M Hoggatt, Sarah L Griffith, Jeanette N McClintick, Patricia Jane Gallagher
OBJECTIVE: Following vascular injury medial smooth muscle cells dedifferentiate and migrate through the internal elastic lamina where they form a neointima. The goal of the current study was to identify changes in gene expression that occur prior to the development of neointima and are associated with the early response to injury. Approach and Results: Vascular injury was induced in C57Bl/6 mice and in Myh11-creER(T2) mTmG reporter mice by complete ligation of the left carotid artery...
December 30, 2016: Physiological Genomics
https://www.readbyqxmd.com/read/28035788/channeling-exciton-migration-into-electron-transfer-in-formamidinium-lead-bromide-perovskite-nanocrystal-fullerene-composites
#19
Vijayakumar C Nair, Chinnadurai Muthu, Andrey L Rogach, Reiko Kohara, Vasudevanpillai Biju
Hydrophobically capped nanocrystals of formamidinium lead bromide (FAPbBr3 ) perovskite (PNC) show bright and stable fluorescence in solution and thin-film states. When compared with isolated PNCs in a solution, close-packed PNCs in a thin film show extended fluorescence lifetime (ca. 4.2 μs), which is due to hopping or migration of photogenerated excitons among PNCs. Both fluorescence quantum efficiency and lifetime decrease in a PNC thin film doped with fullerene (C60 ), which is attributed to channeling of exciton migration into electron transfer to C60 ...
December 30, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/28035737/high-levels-of-diversity-and-population-structure-in-the-potato-late-blight-pathogen-at-the-mexico-center-of-origin
#20
Jianan Wang, Sylvia P Fernândez-Pavía, Meredith M Larsen, Edith Garay-Serrano, Rosario Gregorio-Cipriano, Gerardo Rodríguez-Alvarado, Niklaus J Grünwald, Erica M Goss
Globally destructive crop pathogens often emerge by migrating out of their native ranges. These pathogens are often diverse at their center of origin, and may exhibit adaptive variation in the invaded range via multiple introductions from different source populations. However, source populations are generally unidentified or poorly studied compared to invasive populations. Phytophthora infestans, the causal agent of late blight, is one of the most costly pathogens of potato and tomato worldwide. Mexico is the center of origin and diversity of P...
December 30, 2016: Molecular Ecology
keyword
keyword
84172
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"