keyword
MENU ▼
Read by QxMD icon Read
search

isolation with migration

keyword
https://www.readbyqxmd.com/read/29219948/asperosaponin-vi-promotes-angiogenesis-and-accelerates-wound-healing-in-rats-via-up-regulating-hif-1%C3%AE-vegf-signaling
#1
Cheng-Gui Wang, Yi-Ting Lou, Min-Ji Tong, Li-Lian Zhang, Zeng-Jie Zhang, Yong-Zeng Feng, Shi Li, Hua-Zi Xu, Cong Mao
Wound therapy remains a clinical challenge due to the complexity of healing pathology and high demand of achieving functional and aesthetically satisfactory scars. Newly formed blood vessels are essential for tissue repair since they can support cells at the wound site with nutrition and oxygen. In this study, we investigated the effects of Asperosaponin VI (ASA VI) isolated from a traditional Chinese medicine, the root of Dipsacus asper Wall, in promoting angiogenesis, as well as its function in wound therapeutics...
December 7, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/29218863/population-structure-and-migration-of-the-tobacco-blue-mold-pathogen-peronospora-tabacina-into-north-america-and-europe
#2
Monica Blanco-Meneses, Ignazio Carbone, Jean B Ristaino
Tobacco blue mold, caused by Peronospora tabacina, is an oomycete plant pathogen that causes yearly epidemics in tobacco (Nicotiana tabacum) in the U.S. and Europe. The genetic structure of P. tabacina was examined in order to understand genetic diversity, population structure and patterns of migration. Two nuclear loci, Igs2 and Ypt1 and one mitochondrial locus, cox2, were amplified, cloned and sequenced from fifty four isolates of P. tabacina from the U.S., Central America-Caribbean-Mexico (CCAM), Europe and the Middle East (EULE)...
December 8, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/29215830/-study-of-migration-and-distribution-of-bone-marrow-cells-transplanted-animals-with-b16-melanoma
#3
A F Poveshchenko, A O Solovieva, K E Zubareva, D N Strunkin, O B Gricyk, O V Poveshchenko, A V Shurlygina, V I Konenkov
Purpose. Reveal features migration and distribution of syngeneic bone marrow cells (BMC) and subpopulations (MSC) after transplantation into the recipient carrier B16 melanoma bodies. Methods. We used mouse male and female C57BL/6 mice. Induction of Tumor Growth: B16 melanoma cells implanted subcutaneously into right hind paw of female C57BL/6 mice at a dose of 2.5 x 105 cells / mouse. migration study in vivo distribution and BMC and MSC was performed using genetic markers - Y-chromosome specific sequence line male C57Bl/6 syngeneic intravenous transplantation in females using the polymerase chain reaction (PCR) in real time on Authorized Termal Cycler - Light Cycler 480 II / 96 (Roche)...
April 2017: Patologicheskaia Fiziologiia i èksperimental'naia Terapiia
https://www.readbyqxmd.com/read/29213108/the-potential-therapeutic-effect-of-melatonin-on-human-ovarian-cancer-by-inhibition-of-invasion-and-migration-of-cancer-stem-cells
#4
Maryam Akbarzadeh, Ali Akbar Movassaghpour, Hossein Ghanbari, Maryam Kheirandish, Nazila Fathi Maroufi, Reza Rahbarghazi, Mohammad Nouri, Nasser Samadi
There is an urgent need to identify targeting molecules to control invasion and metastasis in cancer patients. We first isolated cancer stem cells (CSCs) from SKOV3 ovarian cancer cells and then investigated the role of melatonin in invasiveness and migration of CSCs compared to SKOV3 cells. The proportion of CSCs in SKOV3 cells was as low as 1.28% with overexpression of both CD133 and CD44. The ability of spheroid formation along with SOX2 overexpression revealed a high self-renewal potential in isolated cells...
December 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29211342/functional-bkca-channel-in-human-resident-cardiac-stem-cells-expressing-w8b2
#5
Oualid Ayad, Christophe Magaud, Stéphane Sebille, Jocelyn Bescond, Chloé Mimbimi, Christian Cognard, Jean-Francois Faivre, Patrick Bois, Aurelien Chatelier
Recently, a new population of resident cardiac stem cells positive for W8B2 marker has been identified. These cardiac stem cells (CSCs) are considered as an ideal cellular source to repair myocardial damage after infarction. However, the electrophysiological profile of these cells has not been characterized yet. We first establish the conditions of isolation and expansion of W8B2+ CSCs from human heart biopsies using magnetic sorting system followed by flow cytometry cell sorting. These cells display a spindle-shaped morphology, are highly proliferative and possess self-renewal capacity demonstrated by their ability to form colonies...
December 6, 2017: FEBS Journal
https://www.readbyqxmd.com/read/29210369/signatures-of-a-quantum-diffusion-limited-hydrogen-atom-tunneling-reaction
#6
Morgan E Balabanoff, Mahmut Ruzi, David T Anderson
We are studying the details of hydrogen atom (H atom) quantum diffusion in highly enriched parahydrogen (pH2) quantum solids doped with chemical species in an effort to better understand H atom transport and reactivity under these conditions. In this work we present kinetic studies of the 193 nm photo-induced chemistry of methanol (CH3OH) isolated in solid pH2. Short-term irradiation of CH3OH at 1.8 K readily produces CH2O and CO which we detect using FTIR spectroscopy. The in situ photochemistry also produces CH3O and H atoms which we can infer from the post-photolysis reaction kinetics that display significant CH2OH growth...
December 6, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29209821/hemolytic-escherichia-coli-inhibits-swarming-and-differentiation-of-proteus-mirabilis
#7
Zhen Luo, Xulin Xie, Yong Qi, Yong Wu
Swarming is a hallmark of Proteus mirabilis, whether common gram-negative bacilli affect the swarming of P. mirabilis is still unclear. In this study, we found that P. mirabilis swarming was inhibited by Escherichia coli ATCC25922, but was not affected by Klebsiella pneumoniae, Acinetobacter baumannii, or Pseudomonas aeruginosa strains. The migration distance of P. mirabilis when mixed with E. coli ATCC25922 was strongly reduced, and the inhibition of the swarming of P. mirabilis by E. coli ATCC25922 was dependent on cell density...
December 5, 2017: Current Microbiology
https://www.readbyqxmd.com/read/29207978/potential-therapeutic-effects-of-n-butylidenephthalide-from-radix-angelica-sinensis-danggui-in-human-bladder-cancer-cells
#8
Sheng-Chun Chiu, Tsung-Lang Chiu, Sung-Ying Huang, Shu-Fang Chang, Shee-Ping Chen, Cheng-Yoong Pang, Teng-Fu Hsieh
BACKGROUND: N-butylidenephthalide (BP) isolated from Radix Angelica Sinensis (Danggui) exhibits anti-tumorigenic effect in various cancer cells both in vivo and in vitro. The effect of BP in bladder cancer treatment is still unclear and worth for further investigate. METHODS: Changes of patients with bladder cancer after Angelica Sinensis exposure were evaluated by analysis of Taiwan's National Health Insurance Research Database (NHIRD) database. The anti-proliferative effect of BP on human bladder cancer cells was investigated and their cell cycle profiles after BP treatment were determined by flow cytometry...
December 6, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29207077/investigation-of-the-pro-apoptotic-effects-of-arbutin-and-its-acetylated-derivative-on-murine-melanoma-cells
#9
Liyan Jiang, Di Wang, Yongfeng Zhang, Junyang Li, Zhiping Wu, Zhi Wang, Di Wang
Arbutin, a natural polyphenol isolated from the bearberry plant Arctostaphylos uvaursi, possesses whitening and anticancer properties. The effects of arbutin on melanogenesis and its pro-apoptotic effect on B16 murine melanoma cells have not yet been reported. In the present study, acetylated arbutin was prepared in order to improve the biological effects of arbutin, and it was found to significantly inhibit the biosynthesis of melanin and tyrosinase activity compared with parent arbutin in B16 murine melanoma cells...
November 16, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29207050/in-vitro-cell-behaviors-of-bone-mesenchymal-stem-cells-derived-from-normal-and-postmenopausal-osteoporotic-rats
#10
Qian Liu, Xiaoxia Zhang, Yang Jiao, Xin Liu, Yirong Wang, Song-Lun Li, Wei Zhang, Fa-Ming Chen, Yin Ding, Chuan Jiang, Zuolin Jin
Postmenopausal osteoporosis (PMO) increases bone fragility and the risk of fractures, and impairs the healing procedure of bone defects in aged women. The stromal cell‑derived factor-1α (SDF-1α)/CXC chemokine receptor type 4 (CXCR4) axis helps to maintain the biological and physiological functions of bone marrow mesenchymal stem cells (BMSCs) and increase the homing efficiency of BMSCs. The present study aimed to provide insights into the possible association between migration and osteogenic ability and the SDF-1α/CXCR4 axis in BMSCs derived from a rat model of PMO...
November 22, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29204978/role-of-glypican-3-in-the-growth-migration-and-invasion-of-primary-hepatocytes-isolated-from-patients-with-hepatocellular-carcinoma
#11
Mauro Montalbano, Cristiana Rastellini, Joshua T McGuire, Janika Prajapati, Ali Shirafkan, Renza Vento, Luca Cicalese
BACKGROUND: Recently, Glypican-3 (GPC3) has been identified as a potential hepatocellular carcinoma (HCC) diagnostic and/or therapeutic target. GPC3 has been found to be up-regulated in HCC and to be absent in normal and cirrhotic liver. As yet, however, the molecular characteristics of GPC3 and its role in HCC cell physiology and development are still undefined. METHODS: Human hepatocyte cultures were established from 10 HCC patients. Additional liver samples were obtained from 5 patients without cirrhosis and/or HCC...
December 4, 2017: Cellular Oncology (Dordrecht)
https://www.readbyqxmd.com/read/29203586/cooh-terminal-saa1-peptides-fail-to-induce-chemokines-but-synergize-with-cxcl8-and-ccl3-to-recruit-leukocytes-via-fpr2
#12
Mieke De Buck, Mieke Gouwy, Nele Berghmans, Ghislain Opdenakker, Paul Proost, Sofie Struyf, Jo Van Damme
A natural leukocyte chemoattractant was isolated from bovine serum by an established four-step purification procedure. Based on its relative molecular mass of 7287 and NH2-terminal sequence, the protein was identified as a carboxy-terminal peptide of the acute phase protein serum amyloid A (SAA) 1. This SAA1(46-112) fragment and its human equivalent SAA1(47-104) were chemically synthesized. Unlike intact SAA1α, these SAA fragments failed to directly chemoattract neutrophils and monocytes, to induce chemokines and to stimulate downstream ERK signaling in monocytes...
December 4, 2017: Blood
https://www.readbyqxmd.com/read/29202449/clonal-relationships-of-csf-b-cells-in-treatment-naive-multiple-sclerosis-patients
#13
Erica L Eggers, Brady A Michel, Hao Wu, Sheng-Zhi Wang, Carolyn J Bevan, Aya Abounasr, Natalie S Pierson, Antje Bischof, Max Kazer, Elizabeth Leitner, Ariele L Greenfield, Stanislas Demuth, Michael R Wilson, Roland G Henry, Bruce Ac Cree, Stephen L Hauser, H-Christian von Büdingen
A role of B cells in multiple sclerosis (MS) is well established, but there is limited understanding of their involvement during active disease. Here, we examined cerebrospinal fluid (CSF) and peripheral blood (PB) B cells in treatment-naive patients with MS or high-risk clinically isolated syndrome. Using flow cytometry, we found increased CSF lymphocytes with a disproportionate increase of B cells compared with T cells in patients with gadolinium-enhancing (Gd+) lesions on brain MRI. Ig gene heavy chain variable region (Ig-VH) repertoire sequencing of CSF and PB B cells revealed clonal relationships between intrathecal and peripheral B cell populations, which could be consistent with migration of B cells to and activation in the CNS in active MS...
November 16, 2017: JCI Insight
https://www.readbyqxmd.com/read/29198899/sentinel-surveillance-data-from-eritrean-migrants-in-italy-the-theory-of-healthy-migrants
#14
Massimo Ciccozzi, Eleonora Cella, Giancarlo Ceccarelli, Serena Vita, Giordano Dicuonzo, Maurizio Lopalco, Francesca Antonelli, Alessia Conti, Rossella Ottaviani, Marina De Cesaris, Silvia Spoto, Silvia Angeletti
BACKGROUND: Eritrean migrants accounted for the majority of people who drowned in the Mediterranean this year. Recently, data deficit about international migration has been reported. METHODS: One hundred and thirty-three Eritrean migrants, upon their arrival in Italy, were accommodated at the asylum seekers center of Castelnuovo di Porto, together with 25 sanitary workers, and received microbiological surveillance at the Clinical Pathology and Microbiology Unit of the University Campus Bio-Medico of Rome...
December 2, 2017: Travel Medicine and Infectious Disease
https://www.readbyqxmd.com/read/29198711/effects-of-connective-tissue-growth-factor-ctgf-ccn2-on-condylar-chondrocyte-proliferation-migration-maturation-differentiation-and-signalling-pathway
#15
Xin Xing, Zhi Li, Zili Yu, Gu Cheng, Dianqi Li, Zubing Li
CCN2, also known as connective tissue growth factor (CTGF), is a 38 kDa cysteine-rich extracellular matrix protein that regulates a sequence of cellular functions and participates in multiple complex biological processes, such as chondrogenesis and osteogenesis. In the present study, we provided the first evidence describing the physiological role of CCN2 in condylar chondrocyte proliferation, migration, maturation and differentiation. CCN2 was widely expressed throughout the whole layers of condylar cartilage and predominantly distributed in the proliferative zone...
November 30, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29197611/overexpression-of-angiopoietin-1-potentiates-endothelial-progenitor-cells-treatment-of-aneurysm
#16
Ziming Lu, Qiujing Wang, Chengwei Chen, Yuyuan Gao, Jian He, Wenchao Liu, Zhenjun Li, Zhenghui Zhao
OBJECTIVES: To investigate whether Angiopoietin-1(Ang-1) could regulate the endothelial progenitor cells(EPCs) survival and the effect of accelerating intra-aneurysmal organization and occlusion of the aneurysm neck. METHODS: EPCs were isolated from Wistar rats. EPCs were cultured and transfected with lentivirus-Ang-1(Ang-1-EPCs) and lentivirus-NC(NC-EPCs). The effects of Ang-1 on viability and functioning of EPCs were explored via tube formation, migration and MTT assays...
November 29, 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/29196500/reduced-prostaglandin-i2-signaling-in-arid5b-primary-skeletal-muscle-cells-attenuates-myogenesis
#17
Jennifer Murray, Robert H Whitson, Keiichi Itakura
The AT-rich interaction domain (ARID) family of proteins regulates gene expression, development, and differentiation. Although Arid5b has important functions in adipogenesis and chondrogenesis, the role of Arid5b in skeletal muscle myogenesis has not been investigated. Therefore, we isolated primary skeletal muscle cells from Arid5b+/+ and Arid5b-/- mice and characterized differentiation in these cells. We found that Arid5b-/- primary skeletal muscle cells showed differentiation defects and impaired sarcomeric assembly...
December 1, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29196262/collagen-vi-suppresses-fibronectin-induced-enteric-neural-crest-cell-migration-by-downregulation-of-focal-adhesion-proteins
#18
Shoichi Nishida, Hisayoshi Yoshizaki, Yoshitomo Yasui, Tsuyoshi Kuwahara, Etsuko Kiyokawa, Miyuki Kohno
The enteric nervous system (ENS) is a network of neurons and glia that are derived from enteric neural crest cells (ENCCs) and essential for regulating peristaltic activity of the colon. ENCCs migrate along the gastrointestinal tract to form the ENS, and disruption of ENCC motility leads to ENS disorders, such as Hirschsprung's disease. Previous ENCC-transplant experiments show that ENCCs can invade into isolated mouse intestines by age E13.5, but not after E15.5. We hypothesized that altered age-specific micro-environments in the intestine are responsible for ENCC invasion/migration...
November 28, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29196166/1-25-oh-2d3-attenuates-pulmonary-arterial-hypertension-via-microrna-204-mediated-tgfbr2-smad-signaling
#19
Hua Yu, Min Xu, Yizhi Dong, Jingjing Liu, Yaozhe Li, Wei Mao, Jindan Wang, Liangxing Wang
Pulmonary arterial hypertension (PAH) is a devastating disease characterized by high pulmonary artery pressure. It is reported that microRNA-204 (miR-204) plays an important role in the development of PAH. Calcitriol [1,25-dihydroxyvitamin D3, 1,25(OH)2D3] mediates multiple pathophysiological processes. The aim of the current study was to explore the role of 1,25(OH)2D3 in PAH. PAH was induced in rats and rat pulmonary arterial endothelial cells (PAECs) were isolated as in vitro PAH model. The mean pulmonary artery pressure, morphologic changes, and expressions of transforming growth factor-beta1 (Tgfbr2), Smad2/7, alpha smooth muscle actin (α-SMA), and p21 were then measured...
November 28, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29195923/calcific-tendonitis-of-the-rotator-cuff-from-formation-to-resorption
#20
Christelle Darrieutort-Laffite, Frédéric Blanchard, Benoit Le Goff
Calcific tendonitis of the rotator cuff is due to apatite deposits in the shoulder tendons. Patients affected by calcific tendonitis have chronic shoulder pain and disability. Although the disease is frequent, about 10 to 42% of painful shoulders, mechanisms leading to this pathological mineralization are still largely unknown. Research reported in the 90s suggested that the formation of calcific deposits is linked to cells looking like chondrocytes identified around calcium deposits within a fibrocartilage area...
November 28, 2017: Joint, Bone, Spine: Revue du Rhumatisme
keyword
keyword
84172
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"