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https://www.readbyqxmd.com/read/29050240/total-flavones-of-abelmoschus-manihot-enhances-angiogenic-ability-both-in-vitro-and-in-vivo
#1
Lingyi Tang, Wu Pan, Guisong Zhu, Zhihui Liu, Dongling Lv, Meng Jiang
Angiogenesis is a process of new blood vessel formation from pre-existing vessels. It is a normal and vital process in growth and development, as well as in wound healing and in the formation of granulation tissue. Total flavones of Abelmoschus manihot (TFA) are the major constituents of the traditional Chinese herb Abelmoschus manihot L. Medic. The aim of this study is to investigate the effect of TFA on angiogenic ability using human umbilical vein endothelial cells (HUVECs) in vitro and chick chorioallantoic membrane (CAM) in vivo...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29049924/equine-mesenchymal-stem-cells-derived-from-endometrial-or-adipose-tissue-share-significant-biological-properties-but-have-distinctive-pattern-of-surface-markers-and-migration
#2
J Cabezas, D Rojas, F Navarrete, R Ortiz, G Rivera, F Saravia, L Rodriguez-Alvarez, F O Castro
Adult stromal mesenchymal stem cells (MSCs) have been postulated as responsible for cell renewal in highly and continuously regenerative tissues such as the endometrium. MSCs have been identified in the endometrium of many species including humans, rodents, pets and some farm animals, but not in horses. The objective of this work was to isolate such cells from the endometrium of mares and to compare their main biological attributes with horse adipose-derived MSCs. Here we successfully isolated and characterized endometrial MSCs (eMSCs) from mares...
October 6, 2017: Theriogenology
https://www.readbyqxmd.com/read/29048645/establishment-and-characterization-of-human-osteosarcoma-cells-resistant-to-pyropheophorbide-%C3%AE-methyl-ester-mediated-photodynamic-therapy
#3
Yong Tao, Yunsheng Ou, Hang Yin, Yanyang Chen, Shenxi Zhong, Yongjian Gao, Zenghui Zhao, Bin He, Qiu Huang, Qianxing Deng
The present study was performed to establish and characterize new human osteosarcoma cell lines resistant to pyropheophorbide-α methyl ester‑mediated photodynamic therapy (MPPa-PDT). MPPa-PDT-resistant cells are isolated from the human osteosarcoma MG63 and HOS cell lines and two resistant populations were finally acquired, including MG63/PDT and HOS/PDT. Cell Counting Kit-8 (CCK-8) assay was used to determine the MPPa-PDT, cisplatin (CDDP) resistance and proliferation of MG63, MG63/PDT, HOS and HOS/PDT cells...
September 27, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/29044680/effects-of-three-blood-derived-products-on-equine-corneal-cells-an-in-vitro-study
#4
J O Rushton, E Kammergruber, A Tichy, M Egerbacher, B Nell, S Gabner
BACKGROUND: Despite advances in therapy of corneal ulcerative diseases in horses, a vast number of cases require surgical intervention, due to poor response to treatment. Topical application of serum has been used for many years, based on its anti-collagenolytic properties and the presence of growth factors promoting corneal wound healing. However, although other blood derived products i.e. platelet rich plasma (PRP), plasma rich in growth factors (PRGF) have been widely used in equine orthopaedics and in human ophthalmology, no reports of the effects of these blood derived products exist in equine ophthalmology...
October 16, 2017: Equine Veterinary Journal
https://www.readbyqxmd.com/read/29043672/preparation-of-highly-enriched-er-membranes-using-free-flow-electrophoresis
#5
Harriet T Parsons
Free-flow electrophoresis (FFE) is a technique for separation of proteins, peptides, organelles, and cells. With zone electrophoresis (ZE-FFE), organelles are separated according to surface charge. The ER is the only remaining major cellular compartment in Arabidopsis not to have been isolated using density centrifugation, immune-isolation, or any other method previously applied to purification of plant membranes. By using continuous-flow electrophoresis ER vesicles of similar surface charge, which may have been fragmented during cell lysis, can be focused...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29043060/origins-of-the-greenland-shark-somniosus-microcephalus-impacts-of-ice-olation-and-introgression
#6
Ryan P Walter, Denis Roy, Nigel E Hussey, Björn Stelbrink, Kit M Kovacs, Christian Lydersen, Bailey C McMeans, Jörundur Svavarsson, Steven T Kessel, Sebastián Biton Porsmoguer, Sharon Wildes, Cindy A Tribuzio, Steven E Campana, Stephen D Petersen, R Dean Grubbs, Daniel D Heath, Kevin J Hedges, Aaron T Fisk
Herein, we use genetic data from 277 sleeper sharks to perform coalescent-based modeling to test the hypothesis of early Quaternary emergence of the Greenland shark (Somniosus microcephalus) from ancestral sleeper sharks in the Canadian Arctic-Subarctic region. Our results show that morphologically cryptic somniosids S. microcephalus and Somniosus pacificus can be genetically distinguished using combined mitochondrial and nuclear DNA markers. Our data confirm the presence of genetically admixed individuals in the Canadian Arctic and sub-Arctic, and temperate Eastern Atlantic regions, suggesting introgressive hybridization upon secondary contact following the initial species divergence...
October 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/29039573/antitumor-effects-of-baicalin-on-ovarian-cancer-cells-through-induction-of-cell-apoptosis-and-inhibition-of-cell-migration-in%C3%A2-vitro
#7
Chen Gao, Yinglu Zhou, Huatao Li, Xia Cong, Zhongling Jiang, Xin Wang, Rongfeng Cao, Wenru Tian
Baicalin, an active flavone isolated from Scutellaria baicalensis Georgi, has been demonstrated to induce various beneficial biochemical effects such as anti‑inflammatory, anti‑viral, and antitumor effects. However, the antitumor mechanism of baicalin is not well understood. In the present study, baicalin was demonstrated to inhibit the viability and migration of a widely used ovarian cancer cell line, A2780, in a dose‑dependent manner. MTT assays revealed that cell viability significantly decreased in ovarian cancer cells treated with baicalin compared with untreated cells, without effect on normal ovarian cells...
October 10, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29039569/baicalin-inhibits-the-metastasis-of-highly-aggressive-breast-cancer-cells-by-reversing-epithelial-to-mesenchymal-transition-by-targeting-%C3%AE-catenin-signaling
#8
Tao Zhou, Aijie Zhang, Ge Kuang, Xia Gong, Rong Jiang, Dan Lin, Jie Li, Hongzhong Li, Xiang Zhang, Jingyuan Wan, Hongyuan Li
Metastasis is the main cause of death in breast cancer patients, which is due partly to the lack of effective treatment. Baicalin, a flavonoid compound isolated from the roots of Scutellaria lateriflora Georgi (Huang Qin), has recently been confirmed as an effective agent for the treatment of a variety of cancers. Yet, the effects and underlying molecular mechanisms of baicalin in regards to the metastasis of breast cancer remain unclear. In the present study, we found that baicalin had the potential to suppress the migration and invasion of highly aggressive breast cancer cells in a dose-dependent manner but had no impact on the viability of these cancer cells...
September 28, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29038754/histamine-2-receptor-antagonist-famotidine-modulates-cardiac-stem-cell-characteristics-in-hypertensive-heart-disease
#9
Sherin Saheera, Ajay G Potnuri, Renuka Nair
BACKGROUND: Cardiac stem cells (CSCs) play a vital role in cardiac homeostasis. A decrease in the efficiency of cardiac stem cells is speculated in various cardiac abnormalities. The maintenance of a healthy stem cell population is essential for the prevention of adverse cardiac remodeling leading to cardiac failure. Famotidine, a histamine-2 receptor antagonist, is currently used to treat ulcers of the stomach and intestines. In repurposing the use of the drug, reduction of cardiac hypertrophy and improvement in cardiac function of spontaneously hypertensive rats (SHR) was reported by our group...
2017: PeerJ
https://www.readbyqxmd.com/read/29037816/in-vitro-segregation-and-isolation-of-human-pluripotent-stem-cell-derived-neural-crest-cells
#10
Sabine Münst, Philipp Koch, Jaideep Kesavan, Michael Alexander-Mays, Bernhard Münst, Sandra Blaess, Oliver Brüstle
The neural crest (NC) is a transient embryonic cell population with remarkable characteristics. After delaminating from the neural tube, NC cells (NCCs) migrate extensively, populate nearly every tissue of the body and differentiate into highly diverse cell types such as peripheral neurons and glia, but also mesenchymal cells including chondrocytes, osteocytes, and adipocytes. While the NC has been extensively studied in several animal models, little is known about human NC development. A number of methods have been established to derive NCCs in vitro from human pluripotent stem cells (hPSC)...
October 13, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/29037814/piperlongumine-suppresses-bladder-cancer-invasion-via-inhibiting-epithelial-mesenchymal-transition-and-f-actin-reorganization
#11
Di Liu, Xin Yao Qiu, Xi Wu, Dian Xing Hu, Chun Yang Li, Shang Bin Yu, Feng Pan, Xiao Qian Chen
Piperlongumine (PL), a natural alkaloid isolated from longer pepper plants, is recently found to be a potent selective anti-cancer compound. We first tested its anti-cancer effects on bladder cancer, the fifth most common and aggressive cancer worldwide, to further explore the therapeutic spectrum and molecular mechanisms of PL. PL significantly suppressed bladder cancer cell proliferation, the transition of G2/M phase to next phase, migration/invasion in vitro and bladder cancer growth/development in vivo...
October 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29037205/vildagliptin-ameliorates-pulmonary-fibrosis-in-lipopolysaccharide-induced-lung-injury-by-inhibiting-endothelial-to-mesenchymal-transition
#12
Toshio Suzuki, Yuji Tada, Santhi Gladson, Rintaro Nishimura, Iwao Shimomura, Satoshi Karasawa, Koichiro Tatsumi, James West
BACKGROUND: Pulmonary fibrosis is a late manifestation of acute respiratory distress syndrome (ARDS). Sepsis is a major cause of ARDS, and its pathogenesis includes endotoxin-induced vascular injury. Recently, endothelial-to-mesenchymal transition (EndMT) was shown to play an important role in pulmonary fibrosis. On the other hand, dipeptidyl peptidase (DPP)-4 was reported to improve vascular dysfunction in an experimental sepsis model, although whether DPP-4 affects EndMT and fibrosis initiation during lipopolysaccharide (LPS)-induced lung injury is unclear...
October 16, 2017: Respiratory Research
https://www.readbyqxmd.com/read/29036438/a-selective-sphingosine-1-phosphate-receptor-1-agonist-sew-2871-aggravates-gastric-cancer-by-recruiting-myeloid-derived-suppressor-cells
#13
Yujing Zhou, Feng Guo
The immune status of tumor microenvironment in gastric cancer is poorly understood, which limits the development of novel strategies in this field. Sphingosine-1-phosphate (S1P) acts as an immune modulator, but the role of S1P in gastric cancer is elusive. Here, we aim to investigate S1P receptor 1 (S1P1)-mediated effect of S1P in gastric cancer. We generated a xenograft mouse model and used SEW-2871, a S1P1 specific agonist to activate S1P1 signaling. Tumor-infiltrating lymphocytes (TILs) were isolated and analyzed using flow cytometry...
October 3, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/29036328/phylogeography-and-population-genetic-analyses-in-the-iberian-toothcarp-aphanius-iberus-valenciennes-1846-at-different-time-scales
#14
Elena G Gonzalez, Carina Cunha, Hamid R Ghanavi, Francisco J Oliva-Paterna, Mar Torralva, Ignacio Doadrio
Secondary freshwater fish species inhabiting fluctuating and extreme environments are susceptible to changes in dispersion, effective population size, and genetic structure. The Iberian toothcarp Aphanius iberus is an endemic cyprinodontid of the Iberian Peninsula restricted to brackish water of salt marshes and coastal lagoons on the eastern Spanish Mediterranean coast. In this study we analysed mitochondrial cytochrome b DNA and microsatellite variation to evaluate ways in which the processes of extinction, dispersal, and colonization of A...
September 22, 2017: Journal of Heredity
https://www.readbyqxmd.com/read/29035721/cdc42-is-essential-for-the-polarized-movement-and-adhesion-of-human-dental-pulp-stem-cells
#15
Mingwei Li, Liang Ma, Bing Song, Dingyi Yu, Min Xiao, Xiaohan Mei, Huihui Guo, Qing Yu
OBJECTIVE: Stem cell-based tissue repair and regeneration require the regulation of cell migration and adhesion. As a regulator of cell polarization, Cdc42 (cell division control protein 42) plays a basic role at the initial stage of cell migration and adhesion. This study explores the effect of Cdc42 on the polarized migration and adhesion of hDPSCs (human dental pulp stem cells). DESIGN: HDPSCs were isolated from extracted third molars and transfected with siRNA targeted against Cdc42...
October 3, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29034248/misoprostol-inhibits-equine-neutrophil-adhesion-migration-and-respiratory-burst-in-an-in-vitro-model-of-inflammation
#16
Emily Medlin Martin, Rebecca Louise Till, Mary Katherine Sheats, Samuel L Jones
In many equine inflammatory disease states, neutrophil activities, such as adhesion, migration, and reactive oxygen species (ROS) production become dysregulated. Dysregulated neutrophil activation causes tissue damage in horses with asthma, colitis, laminitis, and gastric glandular disease. Non-steroidal anti-inflammatory drugs do not adequately inhibit neutrophil inflammatory functions and can lead to dangerous adverse effects. Therefore, novel therapies that target mechanisms of neutrophil-mediated tissue damage are needed...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/29030089/knockdown-of-hif-1%C3%AE-inhibits-the-proliferation-and-migration-of-outer-root-sheath-cells-exposed-to-hypoxia-in-vitro-an-involvement-of-shh-pathway
#17
Haihua Zhang, Weixiao Nan, Xingchao Song, Shiyong Wang, Huazhe Si, Guangyu Li
AIMS: Outer root sheath (ORS) is a highly proliferative component of a hair follicle. This study is performed to investigate whether hypoxia-induced elevation of hypoxia-inducible factor (HIF)-1α, a transcriptional activator, contributes to the outgrowth of ORS cells in vitro. MAIN METHODS: Hair follicles with intact ORS collected from 4-month old male American minks were cultured in normoxic or hypoxic condition (3% oxygen) for 7days. Primary ORS cells isolated from the mink hair follicles were exposed to hypoxia for 12, 24 or 48h, and their proliferation was analyzed with immunofluorescence assay using anti-proliferating cell nuclear antigen (PCNA) antibody...
October 10, 2017: Life Sciences
https://www.readbyqxmd.com/read/29029093/electronegative-low-density-lipoprotein-l5-induces-adipose-tissue-inflammation-associated-with-metabolic-syndrome
#18
Liang-Yin Ke, Hua-Chen Chan, Hsiu-Chuan Chan, Franklin Chikodi Udo Kalu, Hsiang-Chun Lee, I-Ling Lin, Shih-Jie Jhuo, Wen-Ter Lai, Chen-Rong Tsao, Tatsuya Sawamura, Richard A Dixon, Chu-Huang Chen, Chih-Sheng Chu, Shyi-Jang Shin
Context: Electronegative low-density lipoprotein (LDL) L5 is a naturally occurring, atherogenic entity found at elevated levels in the plasma of patients with metabolic syndrome (MetS) in the absence of elevated plasma LDL levels. Objective: To investigate the role of L5 in the mechanism of adipose tissue inflammation associated with MetS. Patients/Setting: Plasma LDL isolated from MetS patients (n=29) and controls (n=29) with similar plasma LDL levels was separated into 5 subfractions, L1-L5, with increasing electronegativity...
September 28, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29026443/neutrophil-killing-of-staphylococcus-aureus-in-diabetes-obesity-and-metabolic-syndrome-a-prospective-cellular-surveillance-study
#19
Ingrid Lea Scully, Lisa Kristin McNeil, Sudam Pathirana, Christine Lee Singer, Yongdong Liu, Stanley Mullen, Douglas Girgenti, Alejandra Gurtman, Michael W Pride, Kathrin Ute Jansen, Paul L Huang, Annaliesa S Anderson
BACKGROUND: Obesity, metabolic syndrome (MetS), and diabetes are frequent in surgical populations and can enhance susceptibility to postoperative surgical site infections. Reduced neutrophil function has been linked with diabetes and risk of Staphylococcus aureus infection. Therefore, neutrophil function in diabetic and obese subjects (± MetS) was assessed in this prospective serological and cellular surveillance study to determine whether vaccines administered to protect against infections after surgery could be effective in these populations...
2017: Diabetology & Metabolic Syndrome
https://www.readbyqxmd.com/read/29026370/preliminary-molecular-characterization-of-a-proinflammatory-and-nociceptive-molecule-from-the-echinometra-lucunter-spines-extracts
#20
Juliana Mozer Sciani, Bianca Zychar, Luis Roberto Gonçalves, Renata Giorgi, Thiago Nogueira, Daniel Carvalho Pimenta
BACKGROUND: Sea urchins are animals commonly found on the Brazilian shoreline, being Echinometra lucunter the most abundant species. Accidents caused by E. lucunter have been reported as one of the most frequent in Brazil, and are characterized by intense pain and inflammation, consequence of spine puncture in the skin. In order to characterize such toxic effects, we isolated one molecule that caused inflammatory and nociceptive effects. METHODS: E. lucunter specimens were collected without gender distinction...
2017: Journal of Venomous Animals and Toxins Including Tropical Diseases
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