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https://www.readbyqxmd.com/read/29775928/tributyltin-impaired-reproductive-success-in-female-zebrafish-through-disrupting-oogenesis-reproductive-behaviors-and-serotonin-synthesis
#1
Wei-Yang Xiao, Ying-Wen Li, Qi-Liang Chen, Zhi-Hao Liu
Tributyltin (TBT), an organotin acting as aromatase (Cyp19a1) inhibitor, has been found to disrupt gametogenesis and reproductive behaviors in several fish species. However, few studies addressing the mechanisms underlying the impaired gametogenesis and reproduction have been reported. In this study, female adults of zebrafish (Danio rerio) were continuously exposed to two nominal concentrations of TBT (100 and 500 ng/L, actual concentrations: 90.8 ± 1.3 ng/L and 470.3 ± 2.7 ng/L, respectively) for 28 days...
May 12, 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/29775925/exposure-to-tris-1-3-dichloro-2-propyl-phosphate-for-two-generations-decreases-fecundity-of-zebrafish-at-environmentally-relevant-concentrations
#2
Yongkang Zhang, Meng Li, Shuying Li, Qiangwei Wang, Guonian Zhu, Guanyong Su, Robert J Letcher, Chunsheng Liu
Previous studies reported that exposure to environmentally relevant concentrations of TDCIPP significantly decreased the number of cumulative eggs in zebrafish, but effects on the quantity of eggs and sperms remained unknown. Therefore, in this study, effects of TDCIPP on yolk diameter, surface morphology of eggs, sperm density and total motility were evaluated. First generation (F0) zebrafish larvae (Danio rerio) were exposed to 0, 50, 500 or 5000 ng/L tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) from 14 days post fertilization (dpf) to 120 dpf...
May 14, 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/29775544/recovery-of-glucose-from-dried-distiller-s-grain-with-solubles-using-combinations-of-solid-state-fermentation-and-insect-culture
#3
Timothy Howdeshell, Takuji Tanaka
A bioethanol byproduct (dried distiller's grains with solubles; DDGS) contains a high cellulose and starch. We hypothesized combinations of solid-state fermentation (SSF) and digestion by black soldier fly larvae (BSFL) (Hermetia illucens) could increase the recovery of glucose from this byproduct through concentrating and loosening cellulose matrix by their activities. DDGS was individually fermented with Aspergillus niger, Aspergillus fumigatus, Trichoderma koningii, Phanerochaete chrysosporium, or Lactobacillus plantarum...
May 18, 2018: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/29774767/the-role-of-hepatic-antioxidant-capacity-and-hepatobiliary-transporter-in-liver-injury-induced-by-isopsoralen-in-zebrafish-larvae
#4
Y Zhang, Y Zhang, J Li, Y Chen, L Han, Q He, J Chu, K Liu
Isopsoralen is the main component of the Chinese medicine psoralen, which has antitumour activity and can be used for the treatment of osteoporosis. However, the mechanism behind its hepatotoxicity has not yet been elucidated. In this study, the hepatotoxicity of isopsoralen was investigated using zebrafish. Isopsoralen treatment groups of 25, 50 and 100 μM were established. The mortality, liver morphology changes, levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), liver histopathology and mRNA levels of liver injury-related genes in zebrafish larvae were measured...
January 1, 2018: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/29774301/-mansonella-perstans-onchocerca-volvulus-and-strongyloides-stercoralis-infections-in-rural-populations-in-central-and-southern-togo
#5
Francois Korbmacher, Kossi Komlan, Richard G Gantin, Wiyao P Poutouli, Koffi Padjoudoum, Potchoziou Karabou, Peter T Soboslay, Carsten Köhler
Background: Mansonella perstans , Onchocerca volvulus and Strongyloides stercoralis are widespread helminth parasites in the tropics. Their distribution remains difficult to determine as it may change during national disease control programs and with regional mass drug administration (MDA). Epidemiological surveys are of importance to evaluate the geographical distribution of these helminth parasites and the diseases they may cause, however, up to date epidemiological evaluations on M...
May 2018: Parasite Epidemiology and Control
https://www.readbyqxmd.com/read/29774292/evaluation-of-phytosynthesised-silver-nanoparticles-from-leaf-extracts-of-leucas-aspera-and-hyptis-suaveolens-and-their-larvicidal-activity-against-malaria-dengue-and-filariasis-vectors
#6
Devan Elumalai, Maduraiveeran Hemavathi, Chandrasekar Vijayalakshmi Deepaa, Patheri Kunyil Kaleena
The present study deals with the green synthesis of silver nanoparticle from the aqueous leaf extracts of Leucas aspera and Hyptis suaveolens as reducing agent and to investigate the larvicidal activity of synthesized silver nanoparticles. The synthesized silver nanoparticles were characterized by Ultraviolet and visible absorption spectroscopy (UV), Fourier transform-infrared spectroscopy (FT-IR), X-ray spectroscopy (XRD), Field emission scanning electron microscope (FESEM) and High-resonance transmission electron microscopy (HRTEM) analysis...
November 2017: Parasite Epidemiology and Control
https://www.readbyqxmd.com/read/29774286/biological-control-of-anopheles-darlingi-aedes-aegypti-and-culex-quinquefasciatus-larvae-using-shrimps
#7
Willian Marinho Dourado Coelho, Juliana de Carvalho Apolinário Coêlho, Katia Denise Saraiva Bresciani, Wilma Aparecida Starke Buzetti
Mosquitoes can act as vectors of important diseases such as malaria, dengue, Zika virus, yellow fever, Chikungunya and Mayaro fever, in addition to filariasis. The use of insecticides, larvicides, bed nets and repellents, besides the use of drugs as chemoprevention and the treatment of the sick are currently the pillars of the control of these vectors. We studied the biological control against of Anopheles darlingi , Aedes aegypti and Culex quinquefasciatus larvae using shrimps of the species M. pantanalense , M...
August 2017: Parasite Epidemiology and Control
https://www.readbyqxmd.com/read/29773840/rna-seq-and-uhplc-q-tof-ms-based-lipidomics-study-in-lysiphlebia-japonica
#8
Xueke Gao, Junyu Luo, Limin Lü, LiJuan Zhang, Shuai Zhang, Jinjie Cui
Lipids play an important role in energy storage, membrane structure stabilization and signaling. Parasitoids are excellent models to study lipidomics because a majority of them do not accumulate during their free-living life-stage. Studies on parasitoids have mostly focused on the changes in the lipids and gene transcripts in hosts and little attention has been devoted to lipidomics and transcriptomics changes in parasitoids. In this study, a relative quantitative analysis of lipids and their gene transcripts in 3-days-old Lysiphlebia japonica larva (3 days after spawning) and pupae were performed using liquid chromatography, mass spectrometry and RNA-seq...
May 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29773336/seasonal-dynamics-geographical-range-size-hosts-genetic-diversity-and-phylogeography-of-amblyomma-sculptum-in-argentina
#9
Evelina L Tarragona, Patrick S Sebastian, María N Saracho Bottero, Emilia I Martinez, Valeria N Debárbora, Atilio J Mangold, Alberto A Guglielmone, Santiago Nava
The aim of this work was to generate knowledge on ecological aspects of Amblyomma sculptum in Argentina, such as seasonal dynamics, geographical range size, hosts, genetic diversity and phylogeography. Adult and immature A. sculptum ticks were collected in different localities of Argentina to know the geographical range size and hosts. The genetic diversity of this tick was studied through analyses of 16S rDNA sequences. To describe the seasonal dynamics, free-living ticks were monthly collected from October 2013 to October 2015...
April 27, 2018: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29773142/a-climate-driven-model-for-the-dynamics-of-the-free-living-stages-of-cooperia-oncophora
#10
Christian W Sauermann, Dave M Leathwick
Experimental results and published literature data regarding the development, survival and herbage translocation of Cooperia oncophora larvae were used to develop a climate-driven model to simulate the dynamics of the free-living stages. From daily maximum and minimum temperature the model estimated hourly development and survival rates of the pre-infective stages and daily survival of infective third stage larvae (L3) inside the faecal pat and in the herbage. In addition, daily rainfall data were used to calculate the translocation rate of the L3 from the faecal pat into the herbage...
May 15, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29773128/time-and-dose-dependent-development-of-humoral-immune-responses-to-ascaridia-galli-in-experimentally-and-naturally-infected-chickens
#11
Gürbüz Daş, Mark Hennies, Armin Tuchscherer, Matthias Gauly
Factors affecting the development of Ascaridia galli-specific humoral responses and their protective roles are largely unknown. We investigated the effects of time and infection dose on A. galli-specific IgY antibody levels following experimental infection. The acquisition and development of new infections and reinfections were also monitored by using tracer birds. Relationships between the retrospective IgY and the final worm burden of the birds were investigated to determine whether humoral immune responses generated during infection provide protection to the host animal...
May 15, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29773077/aedes-vittatus-in-spain-current-distribution-barcoding-characterization-and-potential-role-as-a-vector-of-human-diseases
#12
Alazne Díez-Fernández, Josué Martínez-de la Puente, Santiago Ruiz, Rafael Gutiérrez-López, Ramón Soriguer, Jordi Figuerola
BACKGROUND: Aedes vittatus is currently found in Africa, Asia and Europe, where it acts as a vector of pathogens causing animal and human diseases (e.g. chikungunya, Zika and dengue). Like other Aedes species, Ae. vittatus is able to breed in artificial containers. The ECDC has recently highlighted the need for molecular tools (i.e. barcoding characterization) that enable Aedes species to be identified in entomological surveys. RESULTS: We sampled mosquito larvae and adults in southern Spain and used a molecular approach to amplify and sequence a fragment of the cytochrome c oxidase subunit 1 gene (barcoding region) of the mosquitoes...
May 18, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29772736/exploring-the-effect-of-phage-therapy-in-preventing-vibrio-anguillarum-infections-in-cod-and-turbot-larvae
#13
Nanna Rørbo, Anita Rønneseth, Panos G Kalatzis, Bastian Barker Rasmussen, Kirsten Engell-Sørensen, Hans Petter Kleppen, Heidrun Inger Wergeland, Lone Gram, Mathias Middelboe
The aquaculture industry is suffering from losses associated with bacterial infections by opportunistic pathogens. Vibrio anguillarum is one of the most important pathogens, causing vibriosis in fish and shellfish cultures leading to high mortalities and economic losses. Bacterial resistance to antibiotics and inefficient vaccination at the larval stage of fish emphasizes the need for novel approaches, and phage therapy for controlling Vibrio pathogens has gained interest in the past few years. In this study, we examined the potential of the broad-host-range phage KVP40 to control four different V...
May 16, 2018: Antibiotics
https://www.readbyqxmd.com/read/29772215/a-new-species-and-a-new-record-of-the-simulium-gomphostilbia-gombakense-species-group-diptera-simuliidae-from-thailand
#14
Hiroyuki Takaoka, Wichai Srisuka, Van Lun Low, Atiporn Saeung
Larvae and adults reared from pupae of Simulium (Gomphostilbia) gombakense Takaoka & Davies from eight streams in five provinces of Thailand were genetically analyzed by using cytochrome c oxidase subunit 1 sequences. The material was composed of four lineages, of which lineages 2, 3 and 4 are genetically closely related to one another, but more distantly related to lineage 1, which is equivalent to typical S. (G.) gombakense from Peninsular Malaysia. Lineages 2, 3 and 4, which are morphologically indistinguishable, are designated as genoforms A, B and C within a species complex of S...
May 14, 2018: Acta Tropica
https://www.readbyqxmd.com/read/29771372/tracking-aedes-aegypti-diptera-culicidae-larval-behavior-across-development-effects-of-temperature-and-nutrients-on-individuals-foraging-behavior
#15
Michael H Reiskind, M Shawn Janairo
Immature mosquitoes alter their foraging behavior in response to variation in nutrients, predators, and temperature, with consequences on the adult stage where pathogens are transmitted. These patterns of behavior have not been described with respect to both developmental stage and environmental variation, nor has behavior been examined within an individual across instars. We hypothesized that individual larvae have distinct behavioral syndromes, and predict that the rank of foraging activity in the third instar will be correlated with foraging activity in the fourth instar for an individual across all conditions...
May 15, 2018: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29770670/-final-evaluation-of-control-program-of-key-parasitic-diseases-in-jinhu-county-2006-2015-jiangsu-province
#16
Sun Dao-Kuan, Zhang Cui-Ping
OBJECTIVE: To evaluate the implementation of the Control Program of Key Parasitic Diseases in Jinhu County from 2006 to 2015, so as to provide the evidence for the future work of prevention and control. METHODS: The historical data about key parasitoses were collected, and the organization management, financial support, capacity building, control effects, and so on were evaluated with the descriptive method. RESULTS: From 2006 to 2015, totally 19 technique educations were conducted, 844...
January 9, 2018: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29770656/-study-on-expression-characteristics-of-cytochrome-p450-genes-in-anopheles-sinensis
#17
Wei Mo, Tang Jian-Xia, Li Ju-Lin, Zhang Mei-Hua, Chen Jing, Xu Sui, Cao Yuan-Yuan, Yang Meng-Meng, Zhu Guo-Ding, Zhou Hua-Yun, Cao Jun
OBJECTIVE: To explore the expression characteristics of cytochrome P450 (CYP) candidate genes (CYP6M3, CYP6Y1, CYP6P5, CYP4H14, CYP4G17, CYP12F16) in Anopheles sinensis . METHODS: The samples were collected from different developmental stages (eggs, larvae, pupae and adult mosquitoes (females and males) ), and different tissues (salivary glands, malpighian tubes, midguts, ovaries, and fat bodies) of An. sinensis and the female adult mosquitoes exposed by different insecticide doses (0, 1...
April 20, 2018: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29769706/red-algal-extracts-from-plocamium-lyngbyanum-and-ceramium-secundatum-stimulate-osteogenic-activities-in-vitro-and-bone-growth-in-zebrafish-larvae
#18
Matthew A Carson, John Nelson, M Leonor Cancela, Vincent Laizé, Paulo J Gavaia, Margaret Rae, Svenja Heesch, Eugene Verzin, Christine Maggs, Brendan F Gilmore, Susan A Clarke
Through the current trend for bioprospecting, marine organisms - particularly algae - are becoming increasingly known for their osteogenic potential. Such organisms may provide novel treatment options for osteoporosis and other musculoskeletal conditions, helping to address their large healthcare burden and the limitations of current therapies. In this study, extracts from two red algae - Plocamium lyngbyanum and Ceramium secundatum - were tested in vitro and in vivo for their osteogenic potential. In vitro, the growth of human bone marrow stromal cells (hBMSCs) was significantly greater in the presence of the extracts, particularly with P...
May 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29769574/honey-bees-consider-larval-nutritional-status-rather-than-genetic-relatedness-when-selecting-larvae-for-emergency-queen-rearing
#19
Ramesh R Sagili, Bradley N Metz, Hannah M Lucas, Priyadarshini Chakrabarti, Carolyn R Breece
In honey bees and many other social insects, production of queens is a vital task, as colony fitness is dependent on queens. The factors considered by honey bee workers in selecting larvae to rear new queens during emergency queen rearing are poorly understood. Identifying these parameters is critical, both in an evolutionary and apicultural context. As female caste development in honey bees is dependent on larval diet (i.e. nutrition), we hypothesized that larval nutritional state is meticulously assessed and used by workers in selection of larvae for queen rearing...
May 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29769397/larval-thymectomy-of-xenopus-laevis
#20
Sara Mashoof, Breanna Breaux, Michael F Criscitiello
In jawed vertebrates from sharks to mammals, the thymus is the primary (or central) lymphoid tissue where T cells develop and mature. The particular stromal cell types, cytokine environment, and tissue organization in the thymus are essential for V(D)J recombination, positive selection for major histocompatibility complex recognition, and negative selection against self-peptide recognition of most αβ T cells. The thymectomy operation on Xenopus tadpole larva described here creates a T-cell-deficient model suitable for many immunology studies...
May 16, 2018: Cold Spring Harbor Protocols
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