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https://www.readbyqxmd.com/read/28738303/bio-accumulation-of-lanthanum-from-lanthanum-modified-bentonite-treatments-in-lake-restoration
#1
G Waajen, F van Oosterhout, M Lürling
Lanthanum (La) modified bentonite (LMB) is one of the available mitigating agents used for the reduction of the phosphorus (P) recycling in eutrophic lakes. The potential toxicity of the La from LMB to aquatic organisms is a matter of concern. In this study the accumulation of La was investigated in the macrophyte Elodea nuttallii, in chironomid larvae and in several fish species during periods up to five years following in situ LMB applications. The application of LMB increased the La concentration of exposed plants and animals...
July 21, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28736927/pituitary-thyroid-axis-development-during-the-larval-juvenile-transition-in-the-pejerrey-odontesthes-bonariensis
#2
T Chalde, L A Miranda
The morphological development of the thyroid gland of pejerrey Odontesthes bonariensis during larval-juvenile transition was studied and related to whole-body concentrations of thyroxine (T4 ) and tri-iodothyronine (T3 ). A complementary (c)DNA fragment of the thyroid-stimulating hormone β-subunit (tshb) was sequenced and transcript levels quantified during this period. Follicles with eosinophilic and T4 -immunoreactive colloids were detected at hatching together with tshb transcript levels and whole-body concentrations of T4 and T3 hormones...
July 23, 2017: Journal of Fish Biology
https://www.readbyqxmd.com/read/28736890/insect-frass-in-stored-cereal-products-as-a-potential-source-of-lactobacillus-sanfranciscensis-for-sourdough-ecosystem
#3
Federica Boiocchi, Davide Porcellato, Lidia Limonta, Claudia Picozzi, Ileana Vigentini, Daria Patrizia Locatelli, Roberto Foschino
AIM: Insect frass samples were collected from Drosophila melanogaster, Plodia interpunctella, Rhyzopertha dominica, Sitophilus granarius, S. oryzae, S. zeamais, Tribolium confusum and T. castaneum to elucidate if they can be the origin of Type I sourdough microorganisms (Lactobacillus sanfranciscensis and Candida milleri). METHODS AND RESULTS: Selective enrichments were carried out to isolate Lactic Acid Bacteria and yeast. A metagenetic analysis, targeted on bacterial 16S rRNA gene and fungal ITS region, was performed by using Illumina MiSeq protocol...
July 23, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28736550/probiotic-enterococcus-mundtii-isolate-protects-the-model-insect-tribolium-castaneum-against-bacillus-thuringiensis
#4
Thorben Grau, Andreas Vilcinskas, Gerrit Joop
Enterococcus mundtii strains isolated from the larval feces of the Mediterranean flour moth Ephestia kuehniella show antimicrobial activity against a broad spectrum of Gram-positive and Gram-negative bacteria. The in vitro probiotic characterization of one isolate revealed a high auto-aggregation score, a hydrophilic cell surface, tolerance for low pH, no hemolytic activity, and susceptibility to all tested antibiotics. We used the red flour beetle Tribolium castaneum, an established model organism, for the in vivo characterization of one probiotic E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28736530/identification-and-expression-profiling-of-chemosensory-genes-in-dendrolimus-punctatus-walker
#5
Su-Fang Zhang, Hui-Hui Liu, Xiang-Bo Kong, Hong-Bin Wang, Fu Liu, Zhen Zhang
Dendrolimus punctatus Walker is a serious pest affecting conifers in southern China. As extensive pesticide spraying is currently required to control D. punctatus, new control strategies are urgently needed. Chemosensory genes represent potential molecular targets for development of alternative pest control strategies, and the expression characteristics of these genes provide an indication of their function. To date, little information is available regarding chemosensory genes in D. punctatus or their expression profiles at different development stages and in various tissues...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28736301/foxa-transcriptional-factor-modulates-insect-susceptibility-to-bacillus-thuringiensis-cry1ac-toxin-by-regulating-the-expression-of-toxin-receptor-abcc2-and-abcc3-genes
#6
Jianghuai Li, Yuemin Ma, Wanli Yuan, Yutao Xiao, Chenxi Liu, Jia Wang, Jianxin Peng, Rong Peng, Mario Soberón, Alejandra Bravo, Yongbo Yang, Kaiyu Liu
Cry toxins produced by Bacillus thuringiensis (Bt) are insecticidal proteins widely used in insect control. Recently, it was shown that ATP-binding cassette transporter proteins (ABC) such as ABCC2, ABCC3, ABCG1 and ABCA2 are implicated in the insecticidal action of Cry toxins as putative receptors. However, the transcriptional regulators involved in the expression of ABC transporter genes remain unknown. Sequence analysis of promoter regions of ABCC2 gene from Helicoverpa armigera and ABCC3 gene from Spodoptera litura Sl-HP cultured cells, revealed the potential participation of Forkhead box protein A (FOXA), a transcription factor that regulates the expression of genes through remodeling chromatin...
July 20, 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28735975/molecular-characterization-and-expression-profiles-of-myosin-essential-light-chain-gene-in-the-pacific-oyster-crassostrea-gigas
#7
Hong Yu, Huijuan Li, Qi Li
In molluscs, muscle contraction is triggered by a direct binding of Ca(2+) to myosin. The myosin essential light chain (MELC) provides the Ca(2+) binding site and is of importance for motor function and regulation. In this study, the complete cDNA sequence of MELC gene of the Pacific oyster, Crassostrea gigas, was obtained, and the expression profiles were performed in different tissues and different embryo-larval development stages. The results showed that the full length of C. gigas MELC (CgMELC) cDNA was 659 bp, containing a 5'-untranslated region of 73 bp, a 3'-untranslated region of 112 bp...
July 20, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28735974/characteristics-of-six-small-heat-shock-protein-genes-from-bactrocera-dorsalis-diverse-expression-under-conditions-of-thermal-stress-and-normal-growth
#8
Wei Dou, Yi Tian, Hong Liu, Yan Shi, Guy Smagghe, Jin-Jun Wang
To explore the functions of small heat shock proteins (sHsps) in relation to thermal stress and development in Bactrocera dorsalis (Hendel), one of the most economically important pest species attacking a wide range of fruits and vegetables, six full-length cDNAs of sHsp genes (BdHsp17.7, 18.4, 20.4, 20.6, 21.6 and 23.8) were cloned, and the expression patterns in different developmental stages and tissues, as well as in response to both thermal and 20-hydroxyecdysone (20E) exposures, were examined using real time quantitative PCR...
July 20, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28735587/black-soldier-fly-as-dietary-protein-source-for-broiler-quails-meat-proximate-composition-fatty-acid-and-amino-acid-profile-oxidative-status-and-sensory-traits
#9
M Cullere, G Tasoniero, V Giaccone, G Acuti, A Marangon, A Dalle Zotte
In the perspective of improving the sustainability of meat production, insects have been rapidly emerging as innovative feed ingredient for some livestock species, including poultry. However, at present, there is still limited knowledge regarding the quality and sensory traits of the derived meat. Therefore, the present study tested the effect of a partial substitution of soya bean meal and oil with defatted black soldier fly (Hermetia illucens) larvae meal (H) in the diet for growing broiler quails (Coturnix coturnix japonica) on meat proximate composition, cholesterol, amino acid and mineral contents, fatty acid profile, oxidative status and sensory characteristics...
July 24, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28735573/chemoattractant-activity-of-tris-hydroxymethyl-aminomethane-for-brugia-pahangi-infective-third-stage-larvae
#10
Y Mitsui, K E Ureña-Tatis, Y Aoki
Urocanic acid (UCA) is known as a major chemoattractant for Strongyloides stercoralis infective third-stage larvae (L3). Since Brugia pahangi is a skin-penetrating parasitic nematode similar to S. stercoralis, UCA was expected to be a chemoattractant for B. pahangi L3. Thus, the chemoattractant activity of UCA for B. pahangi L3 was assessed. The chemotactic responses of B. pahangi L3 to UCA or acetic acid (CH3COOH) dissolved in amine solutions were assessed using an agar-plate assay. A test solution of 200 mm UCA dissolved in aqueous 270 mm tris(hydroxymethyl)aminomethane (Tris) significantly attracted B...
July 24, 2017: Journal of Helminthology
https://www.readbyqxmd.com/read/28735502/regenerative-medicine-in-kidney-disease-where-we-stand-and-where-to-go
#11
REVIEW
Fernanda T Borges, Nestor Schor
The kidney is a complex organ with more than 20 types of specialized cells that play an important role in maintaining the body's homeostasis. The epithelial tubular cell is formed during embryonic development and has little proliferative capacity under physiological conditions, but after acute injury the kidney does have regenerative capacity. However, after repetitive or severe lesions, it may undergo a maladaptation process that predisposes it to chronic kidney injury. Regenerative medicine includes various repair and regeneration techniques, and these have gained increasing attention in the scientific literature...
July 22, 2017: Pediatric Nephrology: Journal of the International Pediatric Nephrology Association
https://www.readbyqxmd.com/read/28735425/influence-of-host-plant-on-thaumetopoea-pityocampa-gut-bacterial-community
#12
Cinzia P Strano, Antonino Malacrinò, Orlando Campolo, Vincenzo Palmeri
Microbial communities associated to the gut of insects are attracting an increasing interest, mainly because of their role in influencing several host life-traits. The characterization of the gut microbial community is pivotal for understanding insect ecology and, thus, to develop novel pest management strategies. The pine processionary moth, Thaumetopoea pytiocampa (Denis & Schiff.) (Lepidoptera: Thaumetopoeidae), is a severe defoliator of pine forests, able to feed on several pine species. In this work, we performed a metabarcoding analysis to investigate, for the first time, the diversity of the gut bacterial community of pine processionary larvae associated with three different host pine species (Pinus halepensis, Pinus nigra subsp...
July 22, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28735242/transcriptomic-analysis-of-the-larva-taenia-multiceps
#13
W H Li, N Z Zhang, L Yue, Y Yang, L Li, H B Yan, T T Li, Z G Qu, W Z Jia, B Q Fu
Taenia multiceps is an adult worm affiliated to Taeniidae family, Platyhelminthes phylum. The larvae of the parasite (Coenurus cerebralis) parasitic in the brain and spinal cord in domestic and wild ruminants or humans can led to a fatal central nervous system (CNS) disease. The aims of the present study were to define the transcriptome profiles of the larvae of T. multiceps by RNA-Seq approach, and to generate large functional gene datasets that could be used to predict the key molecular pathways linked to this cestode...
July 12, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28733328/thermal-imprinting-modifies-bone-homeostasis-in-cold-challenged-sea-bream-sparus-aurata-l
#14
Ana Patrícia Mateus, Rita Costa, Enric Gisbert, Patricia I S Pinto, Karl B Andree, Alicia Estévez, Deborah M Power
Fish are ectotherms and temperature plays a determinant role in their physiology, biology and ecology and is a driver of seasonal responses. The present study assessed how thermal imprinting during embryonic and larval stages modified the response of adult fish to low water temperature. We targeted the gilthead sea bream that develops a condition known as winter syndrome when it is exposed to low water temperatures. Eggs and larvae of sea bream were exposed to four different thermal regimes and then the response of the resulting adults to a low temperature challenge was assessed...
July 21, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28733289/an-enterotoxin-like-binary-protein-from-pseudomonas-protegens-with-potent-nematicidal-activity
#15
Jun-Zhi Wei, Daniel L Siehl, Zhenglin Hou, Barbara Rosen, Jarred Oral, Christopher G Taylor, Gusui Wu
Soil microbes are a major food source for free-living soil nematodes. It is known that certain soil bacteria have evolved systems to combat predation. We identified the nematode-antagonistic Pseudomonas protegens strain 15G2 from screening of microbes. Through protein purification we identified a binary protein, designated as Pp-ANP, that is responsible for the nematicidal activity. This binary protein inhibits Caenorhabditis elegans growth and development by arresting larvae at L1 stage and killing older staged worms...
July 21, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28733240/metabolomics-reveal-physiological-changes-in-mayfly-larvae-neocloeon-triangulifer-at-ecological-upper-thermal-limits
#16
Hsuan Chou, Wimal Pathmasiri, Jocelin Deese-Spruill, Susan Sumner, David B Buchwalter
Aquatic insects play critical roles in freshwater ecosystems and temperature is a fundamental driver of species performance and distributions. However, the physiological mechanisms that determine the thermal performance of species remain unclear. Here we used a metabolomics approach to gain insights into physiological changes associated with a short-term, sublethal thermal challenge in the mayfly Neocloeon triangulifer (Ephemeroptera: Baetidae). Larvae were subjected to a thermal ramp (from 22 to 30 °C at a rate of 1°C/h) and metabolomics analysis (both Nuclear Magnetic Resonance (NMR) Spectroscopy and Gas Chromatography coupled Time-of-Flight Mass Spectrometry (GC-TOF-MS)) indicated that processes related to energetics (sugar metabolism) and membrane stabilization primarily differentiated heat treated larvae from controls...
July 18, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28733238/circadian-regulation-of-caterpillar-feeding-and-growth
#17
Agnieszka Suszczynska, Magdalena Maria Kaniewska, Piotr Bebas, Jadwiga Maria Giebultowicz, Joanna Kotwica-Rolinska
Circadian clocks orchestrate many physiological processes in adult organisms. For example, rhythmic feeding behavior is regulated by the central clock in the nervous system in coordination with metabolic rhythms, which in turn depend mostly on peripheral clocks localized in many tissues. Disruption of the circadian clock leads to metabolic dysregulation both in mammals and in the model insect Drosophila melanogaster. Circadian coordination of feeding and metabolism has been studied mainly in adult insects and not in larval stages, which are dramatically different from adults in species with complete full metamorphosis...
July 18, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28732770/pharmacologic-modeling-of-primary-mitochondrial-respiratory-chain-dysfunction-in-zebrafish
#18
James Byrnes, Rebecca Ganetzky, Richard Lightfoot, Michael Tzeng, Eiko Nakamaru-Ogiso, Christoph Seiler, Marni J Falk
Mitochondrial respiratory chain (RC) disease is a heterogeneous and highly morbid group of energy deficiency disorders for which no proven effective therapies exist. Robust vertebrate animal models of primary RC dysfunction are needed to explore the effects of variation in RC disease subtypes, tissue-specific manifestations, and major pathogenic factors contributing to each disorder, as well as their pre-clinical response to therapeutic candidates. We have developed a series of zebrafish (Danio rerio) models that inhibit, to variable degrees, distinct aspects of RC function, and enable quantification of animal development, survival, behaviors, and organ-level treatment effects on function as well as mitochondrial biochemistry and physiology...
July 18, 2017: Neurochemistry International
https://www.readbyqxmd.com/read/28732753/gene-expression-changes-in-honey-bees-induced-by-sublethal-imidacloprid-exposure-during-the-larval-stage
#19
Ming-Cheng Wu, Yu-Wen Chang, Kuang-Hui Lu, En-Cheng Yang
Honey bee larvae exposed to sublethal doses of imidacloprid show behavioural abnormalities as adult insects. Previous studies have demonstrated that this phenomenon originates from abnormal neural development in response to imidacloprid exposure. Here, we further investigated the global gene expression changes in the heads of newly emerged adults and observed that 578 genes showed more than 2-fold changes in gene expression after imidacloprid exposure. This information might aid in understanding the effects of pesticides on the health of pollinators...
July 18, 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28732272/screening-of-the-open-scaffolds-collection-from-compounds-australia-identifies-a-new-chemical-entity-with-anthelmintic-activities-against-different-developmental-stages-of-the-barber-s-pole-worm-and-other-parasitic-nematodes
#20
Sarah Preston, Yaqing Jiao, Jonathan B Baell, Jennifer Keiser, Simon Crawford, Anson V Koehler, Tao Wang, Moana M Simpson, Ray M Kaplan, Karla J Cowley, Kaylene J Simpson, Andreas Hofmann, Abdul Jabbar, Robin B Gasser
The discovery and development of novel anthelmintic classes is essential to sustain the control of socioeconomically important parasitic worms of humans and animals. With the aim of offering novel, lead-like scaffolds for drug discovery, Compounds Australia released the 'Open Scaffolds' collection containing 33,999 compounds, with extensive information available on the physicochemical properties of these chemicals. In the present study, we screened 14,464 prioritised compounds from the 'Open Scaffolds' collection against the exsheathed third-stage larvae (xL3s) of Haemonchus contortus using recently developed whole-organism screening assays...
May 28, 2017: International Journal for Parasitology, Drugs and Drug Resistance
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