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https://www.readbyqxmd.com/read/28648868/immune-modulating-effects-in-mouse-dendritic-cells-of-lactobacilli-and-bifidobacteria-isolated-from-individuals-following-omnivorous-vegetarian-and-vegan-diets
#1
Diomira Luongo, Lucia Treppiccione, Alida Sorrentino, Ilario Ferrocino, Silvia Turroni, Monica Gatti, Raffaella Di Cagno, Yolanda Sanz, Mauro Rossi
Lactobacilli and bifidobacteria play a primary role in modulation of gut immunity. By considering that microbiota composition depends on various factors, including diet, we asked whether functional differences could characterize faecal populations of lactobacilli and bifidobacteria isolated from individuals with different dietary habits. 155 healthy volunteers who followed omnivorous, ovo-lacto-vegetarian or vegan diets were recruited at four Italian centres (Turin, Parma, Bologna and Bari). Faecal samples were collected; lactobacilli and bifidobacteria were isolated on selective media and their immunomodulatory activity was tested in mouse dendritic cells (DCs)...
June 22, 2017: Cytokine
https://www.readbyqxmd.com/read/28641489/screening-of-several-biological-activities-induced-by-different-sesquiterpene-lactones-isolated-from-centaurea-behen-l-and-rhaponticum-repens-l-hidalgo
#2
Abolfazl Shakeri, Elaheh Amini, Javad Asili, Milena Masullo, Sonia Piacente, Mehrdad Iranshahi
This study aims to evaluate the in vitro cytotoxic, in vitro and in ovo anti-angiogenic effects and antimicrobial activity of sesquiterpene lactones (SLs) from two plants Centaurea behen and Rhaponticum repens (L.). Five SLs, including cynaropicrin (1), 4β,15-dehydro-3-dehydrosolstitialin A (2), aguerin B (3), janerin (4), cebellin E (5), and a flavone hispidulin (6) were isolated from C. behen (compounds 1-3) and R. repens (compounds 4-6). Cynaropicrin (1) and aguerin B (3) were characterised by strong cytotoxic activities against A2780 cells with IC50 values of 1...
June 23, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28641395/effect-of-in-ovo-injection-of-corticotropin-releasing-hormone-on-the-timing-of-hatching-in-broiler-chickens
#3
Yugo Watanabe, Sylvia V H Grommen, Bert De Groef
In chicken embryos, intravenous injection of corticotropin-releasing hormone (CRH) causes the release of both corticosteroids and thyroid hormones. These hormones initiate and enhance the hatching process, raising the possibility that CRH treatment of the late chicken embryo could accelerate hatching and/or decrease the spread of hatching. We performed a series of exploratory tests to investigate whether in ovo delivery methods of CRH other than intravenous injection that are more practical in a commercial setting, affect hatching time in broilers...
June 21, 2017: Poultry Science
https://www.readbyqxmd.com/read/28636279/effects-of-in-ovo-injection-of-chrysin-quercetin-and-ascorbic-acid-on-hatchability-somatic-attributes-hepatic-oxidative-status-and-early-post-hatch-performance-of-broiler-chicks
#4
F Khaligh, A Hassanabadi, H Nassiri-Moghaddam, A Golian, G-A Kalidari
An experiment was conducted to evaluate the effects of in ovo injection of chrysin, quercetin and ascorbic acid on hatchability, somatic attributes, hepatic antioxidant status and early post-hatch growth performance of broiler chicks. Four hundred and eighty embryonated broiler breeder eggs containing live 18-day-old embryos were divided into six groups of 80 eggs each. One group remained intact and served as a control group (i), whereas the other five groups were injected with the prepared injection solutions as follows: (ii) 0...
June 21, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28628658/effects-of-obesogenic-diet-and-estradiol-on-dorsal-raphe-gene-expression-in-old-female-macaques
#5
Cynthia L Bethea, Kevin Mueller, Arubala P Reddy, Steven G Kohama, Henryk F Urbanski
The beneficial effects of bioidentical ovarian steroid hormone therapy (HT) during the perimenopause are gaining recognition. However, the positive effects of estrogen (E) plus or minus progesterone (P) administration to ovariectomized (Ovx) lab animals were recognized in multiple systems for years before clinical trials could adequately duplicate the results. Moreover, very large numbers of women are often needed to find statistically significant results in clinical trials of HT; and there are still opposing results being published, especially in neural and cardiovascular systems...
2017: PloS One
https://www.readbyqxmd.com/read/28619884/effect-of-an-obesogenic-diet-on-circadian-activity-and-serum-hormones-in-old-monkeys
#6
Henryk F Urbanski, Kevin Mueller, Cynthia Bethea
Like women, old female rhesus macaques undergo menopause and show many of the same age-associated changes, including perturbed activity/rest cycles and altered circulating levels of many hormones. Previous studies showed that administration of an estrogen agonist increased activity in female monkeys, that hormone therapy (HT) increased activity in postmenopausal women, and that obesity decreased activity in women. The present study sought to determine if postmenopausal activity and circulating hormone levels also respond to HT when monkeys are fed a high-fat, high-sugar Western-style diet (WSD)...
June 15, 2017: Endocrine Connections
https://www.readbyqxmd.com/read/28613150/toll-like-receptor-tlr-21-signalling-mediated-antiviral-response-against-avian-influenza-virus-infection-correlates-with-macrophage-recruitment-and-nitric-oxide-production
#7
Mohamed Sarjoon Abdul-Cader, Hanaa Ahmed-Hassan, Aruna Amarasinghe, Eva Nagy, Shayan Sharif, Mohamed Faizal Abdul-Careem
Cytosine-guanosinedeoxynucleotide (CpG) DNA can be used for the stimulation of the toll-like receptor (TLR)21 signalling pathway in avian species which ultimately leads to up-regulation of gene transcription for pro-inflammatory molecules including nitric oxide and recruitment of innate immune cells. The objective of this study was to determine the antiviral effect of NO, produced in response to in ovo delivery of CpG DNA, against avian influenza virus (AIV) infection. We found that when CpG DNA is delivered at embryo day (ED)18 in ovo and subsequently challenged with H4N6 AIV at ED19 pre-hatch and day 1 post-hatching, CpG DNA reduces H4N6 AIV replication associated with enhanced NO production and macrophage recruitment in lungs...
June 14, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28608638/effect-of-different-levels-of-copper-nanoparticles-and-copper-sulphate-on-performance-metabolism-and-blood-biochemical-profiles-in-broiler-chicken
#8
A Scott, K P Vadalasetty, M Łukasiewicz, S Jaworski, M Wierzbicki, A Chwalibog, E Sawosz
A study was conducted to investigate the influence of copper administration in ovo to chicken embryos and/or supplied in drinking water to growing chickens in the form copper nanoparticles (Cu-NP) or copper sulphate (CuSO4 ). The fertilised eggs were assigned to three groups (n = 50 per group): control (not injected), injected with 50 mg/kg Cu-NP or with 50 mg/kg CuSO4 at day 1 of incubation. Thereafter, 126 one-day-old broiler chickens were randomly assigned to seven post-hatched groups: control not injected and not provided with Cu in the drinking water, injected with 50 mg/kg Cu-NP + 20 mg/kg in water, not injected + 20 mg/kg Cu-NP in water, injected with 50 mg/kg CuSO4  + 20 mg/kg in water, not injected + 20 mg/kg CuSO4 in water, injected with 50 mg/kg Cu-NP and injected with 50 mg/kg CuSO4 ...
June 13, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28606144/a-reverse-genetics-system-for-avian-coronavirus-infectious-bronchitis-virus-based-on-targeted-rna-recombination
#9
Steven J van Beurden, Alinda J Berends, Annika Krämer-Kühl, Dieuwertje Spekreijse, Gilles Chénard, Hans-Christian Philipp, Egbert Mundt, Peter J M Rottier, M Hélène Verheije
BACKGROUND: Avian coronavirus infectious bronchitis virus (IBV) is a respiratory pathogen of chickens that causes severe economic losses in the poultry industry worldwide. Major advances in the study of the molecular biology of IBV have resulted from the development of reverse genetics systems for the highly attenuated, cell culture-adapted, IBV strain Beaudette. However, most IBV strains, amongst them virulent field isolates, can only be propagated in embryonated chicken eggs, and not in continuous cell lines...
June 12, 2017: Virology Journal
https://www.readbyqxmd.com/read/28589335/a-non-destructive-bfcod-assay-for-in-vivo-measurement-of-cytochrome-p450-3a-cyp3a-enzyme-activity-in-fish-embryos-and-larvae
#10
Elias M Oziolor, Alexis N Carey, Cole W Matson
There is increasing interest in quantifying the exposure and effects of anthropogenic contaminants in fish. Determination of exposures in wild fish is routinely performed, but methods to investigate potential effects are less established. One of the most relevant approaches would be the use of in vivo assays, but existing assays are often limited to in vitro determination of enzyme activity. Many pharmaceuticals and some persistent pollutants activate, and are metabolized by cytochrome P4503A (CYP3A), which make it a relevant and desirable target for biomarker research...
June 6, 2017: Ecotoxicology
https://www.readbyqxmd.com/read/28581358/effect-of-copper-nanoparticles-administered-in-ovo-on-the-activity-of-proliferating-cells-and-on-the-resistance-of-femoral-bones-in-broiler-chickens
#11
Natalia Mroczek-Sosnowska, Monika Łukasiewicz, Dobrochna Adamek, Maciej Kamaszewski, Jan Niemiec, Agnieszka Wnuk-Gnich, Abdullah Scott, André Chwalibog, Ewa Sawosz
The objective of this study was to evaluate bone resistance after in ovo administration of copper nanoparticles (NanoCu) and to determine the number of cells positive for proliferating cell nuclear antigen (PCNA) in the femoral bones of broiler chickens (n = 12 per group). The study demonstrated that femoral bones from the NanoCu group were characterised by a higher weight and volume and by significantly greater resistance to fractures compared to the Control group. NanoCu promoted the proliferation of PCNA-positive cells in the long bones of chickens...
August 2017: Archives of Animal Nutrition
https://www.readbyqxmd.com/read/28580904/fabrication-of-core-shell-nanofibers-for-controlled-delivery-of-bromelain-and-salvianolic-acid-b-for-skin-regeneration-in-wound-therapeutics
#12
Ekambaram Shoba, Rachita Lakra, Manikantan Syamala Kiran, Purna Sai Korrapati
The physiological and pathological complexity of the wound healing process makes it more challenging to design an ideal tissue regeneration scaffold. Precise scaffolding with high drug loading efficiency, efficient intracellular efficacy for therapeutic delivery, minimal nonspecific cellular and blood protein binding, and maximum biocompatibility forms the basis for an ideal delivery system. This paper describes a combinational multiphasic delivery system, where biomolecules are delivered through the fabrication of coaxial electrospinning of different biocompatible polymers...
June 5, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28578423/chicken-ifn-kappa-a-novel-cytokine-with-antiviral-activities
#13
Diwakar Santhakumar, Munir Iqbal, Venugopal Nair, Muhammad Munir
Interferons (IFNs) are essential components of the host innate immune system and define first-line of defence against pathogens. In mammals, several type I IFNs are identified, however, only limited data is available on the repertoire of IFNs in avian species. Here we report the characterization of chicken IFN-κ (chIFN-κ) near the type I IFN locus on the sex-determining Z chromosome. Genetic, evolutionary and syntenic analyses indicate that chIFN-κ is a type I IFN with conserved genetic features and promoter binding sites...
June 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28572591/morphologic-and-biometric-evaluation-of-chick-embryo-eyes-in-ovo-using-7-tesla-mri
#14
Tobias Lindner, Ronja Klose, Felix Streckenbach, Thomas Stahnke, Stefan Hadlich, Jens-Peter Kühn, Rudolf F Guthoff, Andreas Wree, Anne-Marie Neumann, Marcus Frank, Änne Glass, Sönke Langner, Oliver Stachs
The purposes of this study were (1) to characterize embryonic eye development during incubation in ovo and (2) to analyze the putative influence of repetitive ultrahigh-field MRI (UHF-MRI) measurements on this development. A population of 38 fertilized chicken eggs was divided into two sub-groups: two eggs (Group A) were examined repeatedly during the developmental period from embryonic day 1 (E1) to embryonic day 20 (E20) to evaluate the influence of daily MRI scanning. A second larger group of 36 eggs was examined pairwise on one day only, from E3 to E20, and the embryos were sacrificed immediately after MR imaging (Group B)...
June 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28562352/rapalogs-can-promote-cancer-cell-stemness-in-vitro-in-a-galectin-1-and-h-ras-dependent-manner
#15
Itziar M D Posada, Benoit Lectez, Mukund Sharma, Christina Oetken-Lindholm, Laxman Yetukuri, Yong Zhou, Tero Aittokallio, Daniel Abankwa
Currently several combination treatments of mTor- and Ras-pathway inhibitors are being tested in cancer therapy. While multiple feedback loops render these central signaling pathways robust, they complicate drug targeting.Here, we describe a novel H-ras specific feedback, which leads to an inadvertent rapalog induced activation of tumorigenicity in Ras transformed cells. We find that rapalogs specifically increase nanoscale clustering (nanoclustering) of oncogenic H-ras but not K-ras on the plasma membrane...
May 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28558289/toxicity-of-triphenyltin-on-the-development-of-retinal-axons-in-zebrafish-at-low-dose
#16
Yue Xiao, Jieqiong Jiang, Wenxin Hu, Yanbin Zhao, Jianying Hu
The impacts of triphenyltin (TPT) on ecological health are of particular concern due to the unexpectedly high levels found in wild fish around the world. Here, zebrafish embryos were exposed to TPT via in ovo nano-injection to study its toxicity on the development of retinal axons in fish. Lipophilic dye labeling revealed obvious defects in retinal axon development in larvae with normally shaped eyes, with incidences of 0, 1.08%, 2.66%, 4.26%, and 6.85% observed in the control, 0.8, 4.0, 20.0, and 100ng TPT-Cl/g wet weight (ww) exposure groups, respectively, showing a dose-dependent increase...
May 22, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28535001/standardization-of-a375-human-melanoma-models-on-chicken-embryo-chorioallantoic-membrane-and-balb-c-nude-mice
#17
Stefana Avram, Dorina-Elena Coricovac, Ioana Zinuca Pavel, Iulia Pinzaru, Roxana Ghiulai, Flavia Baderca, Codruta Soica, Danina Muntean, Daciana E Branisteanu, Demetrios A Spandidos, Aristides M Tsatsakis, Cristina Adriana Dehelean
Cutaneous melanoma is a metastatic disease characterized by high resistance to treatment, the incidence of which has alarmingly increased worldwide over the past years. A thorough characterization of tumor onset, progression and metastasis is compulsory to overcome the gaps existent in melanoma biology. The present study suggests a well‑established protocol and a detailed histological description of human melanoma models in ovo and in vivo obtained by the inoculation of A375 cells to chick embryo chorioallantoic membrane (CAM) and Balb/c nude mice...
May 22, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28533902/the-influence-of-in-ovo-injection-with-the-prebiotic-dinovo%C3%A2-on-the-development-of-histomorphological-parameters-of-the-duodenum-body-mass-and-productivity-in-large-scale-poultry-production-conditions
#18
Adrianna Sobolewska, Gabriela Elminowska-Wenda, Joanna Bogucka, Agata Dankowiakowska, Anna Kułakowska, Agata Szczerba, Katarzyna Stadnicka, Michał Szpinda, Marek Bednarczyk
BACKGROUND: Among various feed additives currently used in poultry nutrition, an important role is played by bioactive substances, including prebiotics. The beneficial effect of these bioactive substances on the gastrointestinal tract and immune system give rise to improvements in broiler health and performance nutrition, thus increasing the productivity of these birds. An innovative method for introducing bioactive substances into chickens is the in ovo injection into eggs intended for hatching...
2017: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/28526756/restricted-intra-embryonic-origin-of-bona-fide-hematopoietic-stem-cells-in-the-chicken
#19
Laurent Yvernogeau, Catherine Robin
Hematopoietic stem cells (HSCs), responsible for blood cell production, are generated during embryonic development. Human and chicken embryos share features that pose the chicken as a reliable and accessible alternative model to study developmental hematopoiesis. However, the existence of HSCs has never been formally proven in chicken embryos. Here, we established a complete cartography and quantification of hematopoietic cells in the aorta during development. We demonstrated the existence of bona fide HSCs, originating from the chicken embryo aorta (and not the yolk sac, allantois or head), through an in vivo transplantation assay...
May 19, 2017: Development
https://www.readbyqxmd.com/read/28526349/in-vivo-evaluation-of-toxicity-and-antiviral-activity-of-polyrhodanine-nanoparticles-by-using-the-chicken-embryo-model
#20
Ahmad Nazaktabar, Mohammad Soleimani Lashkenari, Atefeh Araghi, Mohsen Ghorbani, Hannaneh Golshahi
Evaluation of the potential cytotoxicity of polyrhodanine nanoparticles is an important factor for its biological applications. In current study, for the first time histopathological and biochemical analysis of polyrhodanine besides of its antiviral activity against Newcastle disease virus (NDV) were examined on chicken embryo model. Polyrhodanine was synthesized by the chemical oxidative polymerization method. The obtained nanoparticles were characterized by scanning electron microscopy (SEM), and Fourier transform infrared (FTIR)...
May 17, 2017: International Journal of Biological Macromolecules
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