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https://www.readbyqxmd.com/read/28430911/a-devices-game-of-thrones-cardiac-resynchronization-therapy-vs-pacemaker
#1
Sara Moura-Ferreira, Helena Gonçalves, Marco Oliveira, João Primo, Paulo Fonseca, José Ribeiro, Elisabeth Santos, Nuno Pelicano, Dinis Martins, Vasco Gama
Aims: Oversensing can interfere with biventricular pacing. Cardiac Resynchronization Therapy (CRT) output inhibition due to automatic brady mode change from a sensing to a pacing mode of a previously implanted pacemaker as it reached battery capacity depleted indicator has not been previously published in the medical literature. Methods and Results: We report the first case of CRT output inhibition in a pacemaker dependent patient due to electrical stimuli from a previously right-sided implanted pacemaker, after unaware reversion of OVO mode (O = no chambers paced; V = ventricular sensing; O = no response to sensing) to backup VVI (V = ventricular pacing; V = ventricular sensing; I = inhibitory response to sensing) when it reached the elective replacement interval...
April 18, 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/28430358/regulation-of-axon-arborization-pattern-in-the-developing-chick-ciliary-ganglion-possible-involvement-of-caspase-3
#2
Hidetaka Katow, Teppei Kanaya, Tomohisa Ogawa, Ryo Egawa, Hiromu Yawo
During a certain critical period in the development of the central and peripheral nervous systems, axonal branches and synapses are massively reorganized to form mature connections. In this process, neurons search their appropriate targets, expanding and/or retracting their axons. Recent work suggested that the caspase superfamily regulates the axon morphology. Here, we tested the hypothesis that caspase 3, which is one of the major executioners in apoptotic cell death, is involved in regulating the axon arborization...
April 21, 2017: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/28419078/targeting-of-ccbe1-by-mir-330-3p-in-human-breast-cancer-promotes-metastasis
#3
Aruz Mesci, Xiaoyong Huang, Samira Taeb, Sahar Jahangiri, Yohan Kim, Emmanouil Fokas, Jeff Bruce, Hon S Leong, Stanley K Liu
BACKGROUND: MicroRNAs (miRs) are involved in the regulation of many processes that contribute to malignancy, including cell proliferation, radiation resistance, invasion and metastasis. The role of miR-330-3p, an miR upregulated in breast cancer, remains unclear. METHODS: We examine the association of miR-330-3p with distant relapse-free survival in the Oxford cohort of breast cancer patients. We also study miR-330-3p function using in vitro invasion and ex ovo metastasis assays...
April 18, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28399949/in-ovo-exposure-to-omega-3-fatty-acids-does-not-enhance-omega-3-long-chain-polyunsaturated-fatty-acid-metabolism-in-broiler-chickens
#4
K Kanakri, J Carragher, B Muhlhausler, R Hughes, R Gibson
The content of omega-3 long-chain polyunsaturated fatty acids (n-3 LCPUFA) in chicken meat can be boosted by feeding broilers a diet containing α-linolenic acid (ALA, from flaxseed oil), some of which is converted by hepatic enzymes to n-3 LCPUFA. However, most of the accumulated n-3 polyunsaturated fatty acid (PUFA) in meat tissues is still in the form of ALA. Despite this, the levels of chicken diets are being enhanced by the inclusion of vegetable and marine sources of omega-3 fats. This study investigated whether the capacity of chicken for n-3 LCPUFA accumulation could be enhanced or inhibited by exposure to an increased supply of ALA or n-3 LCPUFA in ovo...
April 12, 2017: Journal of Developmental Origins of Health and Disease
https://www.readbyqxmd.com/read/28388474/a-comprehensive-quality-evaluation-method-by-ft-nir-spectroscopy-and-chemometric-fine-classification-and-untargeted-authentication-against-multiple-frauds-for-chinese-ganoderma-lucidum
#5
Haiyan Fu, Qiaobo Yin, Lu Xu, Weizheng Wang, Feng Chen, Tianming Yang
The origins and authenticity against frauds are two essential aspects of food quality. In this work, a comprehensive quality evaluation method by FT-NIR spectroscopy and chemometrics were suggested to address the geographical origins and authentication of Chinese Ganoderma lucidum (GL). Classification for 25 groups of GL samples (7 common species from 15 producing areas) was performed using near-infrared spectroscopy and interval-combination One-Versus-One least squares support vector machine (IC-OVO-LS-SVM)...
April 2, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28378120/comprehensive-analysis-of-the-in-vitro-and-ex-ovo-hemocompatibility-of-surface-engineered-iron-oxide-nanoparticles-for-biomedical-applications
#6
Florian Schlenk, Sebastian Werner, Martin Rabel, Franziska Jacobs, Christian Bergemann, Joachim H Clement, Dagmar Fischer
A set of biomedically relevant iron oxide nanoparticles with systematically modified polymer surfaces was investigated regarding their interaction with the first contact partners after systemic administration such as blood cells, blood proteins, and the endothelial blood vessels, to establish structure-activity relationships. All nanoparticles were intensively characterized regarding their physicochemical parameters. Cyto- and hemocompatibility tests showed that (1) the properties of the core material itself were not relevant in short-term incubation studies, and (2) toxicities increased with higher polymer mass, neutral = anionic < cationic surface charge and charge density, as well as agglomeration...
April 4, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28364237/manipulating-gene-expression-in-the-chick-embryo
#7
Octavian Voiculescu, Claudio D Stern
The chick embryo is a well-established representative of amniote embryos, which has been used to make many discoveries, including many major concepts, which have moved our knowledge of developmental biology in hugely important ways. The chick has a relatively compact genome and is easily amenable to embryological manipulations and in vivo imaging. Morpholino gene manipulations have been used in a variety of contexts, and constitute a quick and versatile molecular tool. Here we describe methods to deliver morpholinos to chick embryos, allowing targeting of specific cell populations at defined developmental stages, using two stages as examples: the epiblast of the embryo in the first day of incubation, when the primary germ layers of the embryo are specified, and in ovo electroporation of the neural tube as an example of a later stage...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28347230/inhibition-of-microrna-21-via-locked-nucleic-acid-anti-mir-suppressed-metastatic-features-of-colorectal-cancer-cells-through-modulation-of-programmed-cell-death-4
#8
Reza Nedaeinia, Mohammadreza Sharifi, Amir Avan, Mohammad Kazemi, Abdolreza Nabinejad, Gordon A Ferns, Majid Ghayour-Mobarhan, Rasoul Salehi
Colorectal cancer is among the most lethal of malignancies, due to its propensity to metastatic spread and multifactorial-chemoresistance. The latter property supports the need to identify novel therapeutic approaches for the treatment of colorectal cancer. MicroRNAs are endogenous non-coding small RNA molecules that function as post-transcriptional regulators of gene expression. Recently, programmed cell death 4 has been identified as a protein that increases during apoptosis. This gene is among the potential targets of miR-21 (OncomiR)...
March 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28339834/effects-of-coccidiosis-vaccination-administered-by-in-ovo-injection-on-ross-708-broiler-performance-through-14-days-of-post-hatch-age
#9
A O Sokale, W Zhai, L M Pote, C J Williams, E D Peebles
Effects of the in ovo injection of a commercial coccidiosis vaccine on various hatching chick quality variables and 14 d post-hatch (dph) oocyst shedding have been previously examined. The current study was designed to examine the performance of Ross 708 broilers during the 14 dph period of oocyst shedding following the application of the coccidiosis vaccine. On each of 7 replicate tray levels of a single-stage incubator, a total of 4 treatment groups was randomly represented, with each treatment group containing 63 eggs...
February 22, 2017: Poultry Science
https://www.readbyqxmd.com/read/28339753/in-ovo-monochromatic-green-light-photostimulation-enhances-embryonic-somatotropic-axis-activity
#10
L Dishon, N Avital-Cohen, D Malamud, R Heiblum, S Druyan, T E Porter, M Gumulka, I Rozenboim
Previous studies demonstrated that in ovo photostimulation with monochromatic green light increases body weight and accelerates muscle development in broilers. The mechanism in which in ovo photostimulation accelerates growth and muscle development is not clearly understood. The objective of the current study was to define development of the somatotropic axis in the broiler embryo associated with in ovo green light photostimulation. Two-hundred-forty fertile broiler eggs were divided into 2 groups. The first group was incubated under intermittent monochromatic green light using light-emitting diode (LED) lamps with an intensity of 0...
February 23, 2017: Poultry Science
https://www.readbyqxmd.com/read/28335485/intra-amniotic-administration-of-raffinose-and-stachyose-affects-the-intestinal-brush-border-functionality-and-alters-gut-microflora-populations
#11
Sarina Pacifici, Jaehong Song, Cathy Zhang, Qiaoye Wang, Raymond P Glahn, Nikolai Kolba, Elad Tako
This study investigates the effectiveness of two types of prebiotics-stachyose and raffinose-which are present in staple food crops that are widely consumed in regions where dietary Fe deficiency is a health concern. The hypothesis is that these prebiotics will improve Fe status, intestinal functionality, and increase health-promoting bacterial populations in vivo (Gallus gallus). By using the intra-amniotic administration procedure, prebiotic treatment solutions were injected in ovo (day 17 of embryonic incubation) with varying concentrations of a 1...
March 19, 2017: Nutrients
https://www.readbyqxmd.com/read/28335090/in-ovo-vaccination-using-eimeria-profilin-and-clostridium-perfringens-netb-proteins-in-montanide-ims-adjuvant-increases-protective-immunity-against-experimentally-induced-necrotic-enteritis
#12
Hyun Soon Lillehoj, Seung Ik Jang, Alfredo Panebra, Erik Peter Lillehoj, Laurent Dupuis, Juliette B Arous, Seung Kyoo Lee, Sung Taek Oh
Objective: The effects of vaccinating 18-day-old chicken embryos with the combination of recombinant Eimeria profilin plus Clostridium perfringens NetB proteins mixed in the Montanide IMS adjuvant on the chicken immune response to necrotic enteritis (NE) were investigated using an E. maxima/C. perfringens co-infection NE disease model that we previously developed. Methods: Eighteen-day-old broiler embryos were injected with 100 μl of PBS, profilin, profilin plus NetB, profilin plus NetB/Montanide adjuvant (IMS 106), and profilin plus Net-B/Montanide adjuvant (IMS 101)...
March 21, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28293225/bacterial-composition-genotoxicity-and-cytotoxicity-of-fecal-samples-from-individuals-consuming-omnivorous-or-vegetarian-diets
#13
Ermanno Federici, Roberta Prete, Camilla Lazzi, Nicoletta Pellegrini, Massimo Moretti, Aldo Corsetti, Giovanni Cenci
This study analyzes the composition of viable fecal bacteria and gut toxicology biomarkers of 29 healthy volunteers, who followed omnivorous, lacto-ovo-vegetarian, or vegan diets. In particular, the research was focused on the prevalence of some representative viable bacteria from the four dominant phyla (Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria) commonly present in human feces, in order to evaluate the relationship between microorganisms selected by the habitual dietary patterns and the potential risk due to fecal water (FW) genotoxicity and cytotoxicity, considered as biomarkers for cancer risk and protective food activity...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28289145/irf6-promotes-keratinocyte-differentiation-in-response-to-porphyromonas-gingivalis
#14
Jennifer Huynh, Glen M Scholz, Jiamin Aw, Eric C Reynolds
We recently demonstrated that the expression of the Interferon regulatory factor 6 (IRF6) transcription factor in oral keratinocytes was stimulated by the periodontal pathogen Porphyromonas gingivalis Here, we have established that IRF6 promotes the differentiation of oral keratinocytes in response to P. gingivalis This was evidenced by the IRF6-dependent upregulation of specific markers of keratinocyte terminal differentiation (e.g. Involucrin, IVL; Keratin 13, KRT13), together with additional transcriptional regulators of keratinocyte differentiation, including Grainyhead-like 3 (GRHL3) and Ovo-like zinc finger 1 (OVOL1)...
March 13, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28280715/enterotype-may-drive-the-dietary-associated-cardiometabolic-risk-factors
#15
Ana C F de Moraes, Gabriel R Fernandes, Isis T da Silva, Bianca Almeida-Pititto, Everton P Gomes, Alexandre da Costa Pereira, Sandra R G Ferreira
Analyses of typical bacterial clusters in humans named enterotypes may facilitate understanding the host differences in the cardiometabolic profile. It stills unknown whether the three previously described enterotypes were present in populations living below the equator. We examined how the identification of enterotypes could be useful to explain the dietary associations with cardiometabolic risk factors in Brazilian subjects. In this cross-sectional study, a convenience sample of 268 adults (54.2% women) reported their dietary habits and had clinical and biological samples collected...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28245533/neuregulin1-fine-tunes-pre-post-and-perisynaptic-neuromuscular-junction-development
#16
Jiajing Wang, Fei Song, Jeffrey A Loeb
BACKGROUND: Neuromuscular junction (NMJ) development is a multistep process mediated by coordinated interactions between the nerve terminal, target muscle, and perisynaptic Schwann cell that require constant back-and-forth communication. Retrograde and anterograde growth and differentiation factors have been postulated to participate in this communication. While neuregulin1 (NRG1) has been shown to be potent anterograde signal that activates acetylcholine receptor (AChR) transcription and clustering in vitro, its roles in NMJ development in vivo remain elusive...
February 28, 2017: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/28238175/classification-of-multi-class-motor-imagery-with-a-novel-hierarchical-svm-algorithm-for-brain-computer-interfaces
#17
Enzeng Dong, Changhai Li, Liting Li, Shengzhi Du, Abdelkader Nasreddine Belkacem, Chao Chen
Pattern classification algorithm is the crucial step in developing brain-computer interface (BCI) applications. In this paper, a hierarchical support vector machine (HSVM) algorithm is proposed to address an EEG-based four-class motor imagery classification task. Wavelet packet transform is employed to decompose raw EEG signals. Thereafter, EEG signals with effective frequency sub-bands are grouped and reconstructed. EEG feature vectors are extracted from the reconstructed EEG signals with one versus the rest common spatial patterns (OVR-CSP) and one versus one common spatial patterns (OVO-CSP)...
February 25, 2017: Medical & Biological Engineering & Computing
https://www.readbyqxmd.com/read/28219475/effects-of-in-ovo-feeding-of-creatine-pyruvate-on-the-hatchability-growth-performance-and-energy-status-in-embryos-and-broiler-chickens
#18
M M Zhao, T Gao, L Zhang, J L Li, P A Lv, L L Yu, F Gao, G H Zhou
The effects of in ovo feeding (IOF) of creatine pyruvate (CrPyr) on the hatchability, growth performance and energy status of embryos and broilers (Arbor Acres) were investigated. Five treatments were arranged as non-injected treatment (Control), 0.6 ml physiological saline (0.75%) injected treatment (Saline), and IOF treatments injected with 0.6 ml physiological saline (0.75%) containing 3, 6 or 12 mg CrPyr (CrPyr3, CrPyr6 or CrPyr12) into the amnion per fertile egg on day 17.5 of incubation. After hatching, 80 male chicks from each treatment with similar weight close to the average BW of their pooled group were selected and randomly assigned into eight replicates of 10 chicks each...
February 21, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28215692/developing-a-puncture-free-in-ovo-chicken-transfection-strategy-based-on-bypassing-albumen-nucleases
#19
Hamid-Reza Amini, Abbas Pakdel, Hossein Moradi Shahr-Babak, Shahin Eghbalsaied
Chicken is a dual-purpose animal important from both agricultural and medical aspects. Even though significant improvements have been made in chicken transgenesis technologies, chicken genome manipulation has not been widely used in developmental biology. This study was aimed to evaluate chicken egg white nuclease properties and thereof plausibility of devising an in vivo transfection technology without causing physical damage to the embryo. First, the nuclease activity of egg albumen was assessed. The egg white nucleases were strongly active in degrading DNA and RNA...
March 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28183698/constitutively-active-notch1-converts-cranial-neural-crest-derived-frontonasal-mesenchyme-to-perivascular-cells-in-vivo
#20
Sophie R Miller, Surangi N Perera, Clare V H Baker
Perivascular/mural cells originate from either the mesoderm or the cranial neural crest. Regardless of their origin, Notch signalling is necessary for their formation. Furthermore, in both chicken and mouse, constitutive Notch1 activation (via expression of the Notch1 intracellular domain) is sufficient in vivo to convert trunk mesoderm-derived somite cells to perivascular cells, at the expense of skeletal muscle. In experiments originally designed to investigate the effect of premature Notch1 activation on the development of neural crest-derived olfactory ensheathing glial cells (OECs), we used in ovo electroporation to insert a tetracycline-inducible NotchΔE construct (encoding a constitutively active mutant of mouse Notch1) into the genome of chicken cranial neural crest cell precursors, and activated NotchΔE expression by doxycycline injection at embryonic day 4...
March 15, 2017: Biology Open
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