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https://www.readbyqxmd.com/read/28219475/effects-of-in-ovo-feeding-of-creatine-pyruvate-on-the-hatchability-growth-performance-and-energy-status-in-embryos-and-broiler-chickens
#1
M M Zhao, T Gao, L Zhang, J L Li, P A Lv, L L Yu, F Gao, G H Zhou
The effects of in ovo feeding (IOF) of creatine pyruvate (CrPyr) on the hatchability, growth performance and energy status of embryos and broilers (Arbor Acres) were investigated. Five treatments were arranged as non-injected treatment (Control), 0.6 ml physiological saline (0.75%) injected treatment (Saline), and IOF treatments injected with 0.6 ml physiological saline (0.75%) containing 3, 6 or 12 mg CrPyr (CrPyr3, CrPyr6 or CrPyr12) into the amnion per fertile egg on day 17.5 of incubation. After hatching, 80 male chicks from each treatment with similar weight close to the average BW of their pooled group were selected and randomly assigned into eight replicates of 10 chicks each...
February 21, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28215692/developing-a-puncture-free-in-ovo-chicken-transfection-strategy-based-on-bypassing-albumen-nucleases
#2
Hamid-Reza Amini, Abbas Pakdel, Hossein Moradi Shahr-Babak, Shahin Eghbalsaied
Chicken is a dual-purpose animal important from both agricultural and medical aspects. Even though significant improvements have been made in chicken transgenesis technologies, chicken genome manipulation has not been widely used in developmental biology. This study was aimed to evaluate chicken egg white nuclease properties and thereof plausibility of devising an in vivo transfection technology without causing physical damage to the embryo. First, the nuclease activity of egg albumen was assessed. The egg white nucleases were strongly active in degrading DNA and RNA...
March 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28183698/constitutively-active-notch1-converts-cranial-neural-crest-derived-frontonasal-mesenchyme-to-perivascular-cells-in-vivo
#3
Sophie R Miller, Surangi N Perera, Clare V H Baker
Perivascular/mural cells originate from either the mesoderm or the cranial neural crest. Regardless of their origin, Notch signalling is necessary for their formation. Furthermore, in both chicken and mouse, constitutive Notch1 activation (via expression of the Notch1 intracellular domain) is sufficient in vivo to convert trunk mesoderm-derived somite cells to perivascular cells, at the expense of skeletal muscle. In experiments originally designed to investigate the effect of premature Notch1 activation on the development of neural crest-derived olfactory ensheathing glial cells (OECs), we used in ovo electroporation to insert a tetracycline-inducible NotchΔE construct (encoding a constitutively active mutant of mouse Notch1) into the genome of chicken cranial neural crest cell precursors, and activated NotchΔE expression by doxycycline injection at embryonic day 4...
February 9, 2017: Biology Open
https://www.readbyqxmd.com/read/28183170/physico-chemical-properties-of-late-incubation-egg-amniotic-fluid-and-a-potential-in-ovo-feed-supplement
#4
Apeh A Omede, Momenuzzaman M Bhuiyan, Fakrul A Islam, Paul A Iji
This study explored the physico-chemical properties of late-incubation egg amniotic fluid and a potential in ovo feed (IOF) supplement. Amniotic fluid was collected from broiler breeders (Ross 308, 51 weeks and Cobb 500, 35 weeks) on day 17 after incubation. A mixture of high-quality soy protein supplement - Hamlet Protein AviStart (HPA) was serially diluted in MilliQ water to obtain solutions ranging from 150 to 9.375 mg/ml. The mixtures were heat-treated (0, 30, 60 minutes) in a waterbath (80 oC) and then centrifuged to obtain supernatants...
January 26, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28166978/growth-performance-parameters-bone-calcification-and-immune-response-of-in-ovo-injection-of-25-hydroxycholecalciferol-and-vitamin-k3-in-male-ross-308-broilers
#5
Tahereh Abbasi, Malak Shakeri, Mojtaba Zaghari, Hamid Kohram
This experiment aimed to evaluate the effects of in ovo injection of 25-hydroxycholecalciferol (25-OH-D3) and Vitamin K3 on growth performance, bone calcification and immune system responses in male Ross 308 broilers. Twelve treatment groups with a total number of 768 experimental hatching eggs, four replications and 16 eggs in each replication were selected to form a completely randomized design of factorial arrangement. Treatments included: (1) distilled water, (2) 0.4 μg D3, (3) 0.4 μg D3 + 2 μg K3, (4) 0...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28160004/in-ovo-evaluation-of-floramax%C3%A2-b11-on-marek%C3%A2-s-disease-hvt-vaccine-protective-efficacy-hatchability-microbiota-composition-morphometric-analysis-and-salmonella-enteritidis-infection-in-broiler-chickens
#6
K D Teague, L E Graham, J R Dunn, H H Cheng, N Anthony, J D Latorre, A Menconi, R E Wolfenden, A D Wolfenden, B D Mahaffey, M Baxter, X Hernandez-Velasco, R Merino-Guzman, L R Bielke, B M Hargis, G Tellez
No abstract text is available yet for this article.
February 4, 2017: Poultry Science
https://www.readbyqxmd.com/read/28159858/transcriptomic-alterations-in-the-brain-of-painted-turtles-chrysemys-picta-developmentally-exposed-to-bisphenol-a-or-ethinyl-estradiol
#7
Lindsey K Manshack, Caroline M Conard, Sara J Bryan, Sharon L Deem, Dawn K Holliday, Nathan J Bivens, Scott A Givan, Cheryl Susan Rosenfeld
Developmental exposure of turtles and other reptiles to endocrine disrupting chemicals (EDCs), including bisphenol A (BPA) and ethinyl estradiol (EE), can stimulate partial to full gonadal sex-reversal in males. We have also recently shown that in ovo exposure to either EDC can induce similar sex-dependent behavioral changes typified by improved spatial learning and memory or possibly feminized brain responses. Observed behavioral changes are presumed to be due to BPA- and EE-induced brain transcriptomic alterations during development...
February 3, 2017: Physiological Genomics
https://www.readbyqxmd.com/read/28158826/in-ovo-supplementation-of-probiotics-and-its-effects-on-performance-and-immune-related-gene-expression-in-broiler-chicks
#8
Chasity M Pender, Sungwon Kim, Tiffany D Potter, Miranda M Ritzi, Mark Young, Rami A Dalloul
No abstract text is available yet for this article.
October 27, 2016: Poultry Science
https://www.readbyqxmd.com/read/28128767/chronic-neurodegenerative-illnesses-and-epilepsy-in-danish-adventists-and-baptists-a-nationwide-cohort-study
#9
Lau Caspar Thygesen, Louise NØrreslet Gimsing, Andrea Bautz, Niels Christian Hvidt, Christoffer Johansen
BACKGROUND: Limited knowledge of the influence of lifestyle risk factors and religious living on chronic neurological diseases exist. Seventh-day Adventists (SDA) do not consume tobacco, alcohol, or pork, and many adhere to lacto-ovo-vegetarian diet, and Baptists discourage excessive use of alcohol and tobacco. OBJECTIVE: We investigated whether the incidence of four common chronic neurological illnesses: dementia, Alzheimer's disease, Parkinson's disease, and epilepsy in a large cohort of Danish Adventists and Baptists was different compared to the general Danish population...
January 23, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28108667/effects-on-circulating-steroid-hormones-and-gene-expression-along-the-hypothalamus-pituitary-gonadal-axis-in-adult-japanese-quail-exposed-to-17%C3%AE-trenbolone-across-multiple-generations
#10
Natalie K Karouna-Renier, Yu Chen, Paula F P Henry, Catherine M Maddox, Daniel T Sprague
We investigated the effects of the androgenic growth promoter 17ß-trenbolone (17βTB) on adult Japanese quail (Coturnix japonica) exposed across three generations. The F0 generation was exposed after sexual maturity to 0, 1, 5, 10, 20, and 40 ppm through feed. The F1 generation was exposed in ovo by maternal transfer and through feed at the same doses as their parents. The F2 generation was exposed in ovo only. Levels of plasma sex steroids, gonadal Cytochrome P450 aromatase (CYP19A1) mRNA and select brain neuroendocrine peptide mRNAs were measured...
January 20, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28089549/modified-live-infectious-bursal-disease-virus-ibdv-vaccine-delays-infection-of-neonatal-broiler-chickens-with-variant-ibdv-compared-to-turkey-herpesvirus-hvt-ibdv-vectored-vaccine
#11
Shanika Kurukulasuriya, Khawaja Ashfaque Ahmed, Davor Ojkic, Thushari Gunawardana, Kalhari Goonewardene, Ashish Gupta, Betty Chow-Lockerbie, Shelly Popowich, Philip Willson, Suresh K Tikoo, Susantha Gomis
Chickens are commonly processed around 35-45days of age in broiler chicken industry hence; diseases that occur at a young age are of paramount economic importance. Early age infection with infectious bursal disease virus (IBDV) results in long-lasting immunosuppression and profound economic losses. To our knowledge, this is the first study comparing the protection efficacy of modified live (MdLV) IBDV and herpesvirus turkey (HVT)-IBDV vaccines against early age variant IBDV (varIBDV) infection in chicks. Experiments were carried out in IBDV maternal antibody (MtAb) positive chicks (n=330), divided into 6 groups (n=50-60/group), namely Group 1 (saline), Group 2 (saline+varIBDV), Group 3 (HVT-IBDV), Group 4 (HVT-IBDV+varIBDV), Group 5 (MdLV) and Group 6 (MdLV+varIBDV)...
January 12, 2017: Vaccine
https://www.readbyqxmd.com/read/28087147/immune-responses-to-in-ovo-vaccine-formulations-containing-inactivated-fowl-adenovirus-8b-with-poly-di-sodium-carboxylatoethylphenoxy-phosphazene-pcep-and-avian-beta-defensin-as-adjuvants-in-chickens
#12
Mishal Sarfraz, Muhammed Suleman, Suresh K Tikoo, Colette Wheler, Andrew A Potter, Volker Gerdts, Arshud Dar
Inclusion body hepatitis (IBH) is one of the major viral infections causing substantial economic loss to the global poultry industry. The disease is characterized by a sudden onset of mortality (2-30%) and high morbidity (60-70%). IBH is caused by a number of serotypes of fowl adenovirus with substantially low levels of serotype cross protection. Thus far, there is no effective and safe vaccine commercially available in the North America for the control of IBH in chickens. Poly[di(sodium carboxylatoethylphenoxy)]phosphazene (PCEP) is a high molecular weight, biodegradable water soluble polymer that has been well characterized as a safe and effective adjuvant for a number of experimental veterinary vaccines...
February 7, 2017: Vaccine
https://www.readbyqxmd.com/read/28077286/assessment-of-neuroanatomical-and-behavioural-effects-of-in-ovo-methylmercury-exposure-in-zebra-finches-taeniopygia-guttata
#13
Maria S Yu, Margaret L Eng, Tony D Williams, Mélanie F Guigueno, John E Elliott
Methylmercury (MeHg) readily crosses the blood brain barrier and is a known neuro-toxicant. MeHg accumulation in the brain causes histopathological alterations, neurobehavioral changes, and impairments to cognitive motor functions in mammalian models. However, in birds the neurotoxic effects of MeHg on the developing pre-hatching brain and consequent behavioral alterations in adult birds have not received much attention. Moreover, passerine birds are poorly represented in MeHg neurotoxicology studies in comparison to other avian orders...
January 7, 2017: Neurotoxicology
https://www.readbyqxmd.com/read/28060338/a-submerged-filter-paper-sandwich-for-long-term-ex-ovo-time-lapse-imaging-of-early-chick-embryos
#14
Manuel Schmitz, Ben K A Nelemans, Theodoor H Smit
Due to its availability, low cost, flat geometry, and transparency, the ex ovo chick embryo has become a major vertebrate animal model for the study of morphogenetic events, such as gastrulation(2), neurulation(3)(-)(5), somitogenesis(6), heart bending(7,8), and brain formation(9)(-)(13), during early embryogenesis. Key to understanding morphogenetic processes is to follow them dynamically by time-lapse imaging. The acquisition of time-lapse movies of chick embryogenesis ex ovo has been limited either to short time windows or to the need for an incubator to control temperature and humidity around the embryo(14)...
December 28, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28059170/in-ovo-injection-of-betaine-alleviates-corticosterone-induced-fatty-liver-in-chickens-through-epigenetic-modifications
#15
Yun Hu, Qinwei Sun, Jie Liu, Yimin Jia, Demin Cai, Abdulrahman A Idriss, Nagmeldin A Omer, Ruqian Zhao
Betaine alleviates high-fat diet-induced fatty liver and prenatal betaine programs offspring hepatic lipid metabolism. Excessive corticosterone (CORT) exposure causes fatty liver in chickens, yet it remains unknown whether and how prenatal betaine modulates the susceptibility of CORT-induced fatty liver later in life. In this study, fertilized eggs were injected with saline or betaine before incubation, and the hatchlings were raised at 8 weeks of age followed by 7 days of subcutaneous CORT injection. CORT-induced fatty liver was less severe in betaine-treated chickens, with significantly reduced oil-red staining and hepatic triglyceride content (P < 0...
January 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28059165/conserved-role-of-ovo-in-germline-development-in-mouse-and-drosophila
#16
Makoto Hayashi, Yuko Shinozuka, Shuji Shigenobu, Masanao Sato, Michihiko Sugimoto, Seiji Ito, Kuniya Abe, Satoru Kobayashi
Ovo, which encodes a transcription factor with Zn-finger domains, is evolutionarily conserved among animals. In Drosophila, in addition to its zygotic function for egg production, maternal ovo activity is required in primordial germ cells (PGCs) for expression of germline genes such as vasa and nanos. In this study, we found that maternal Ovo accumulates in PGC nuclei during embryogenesis. In these cells, ovo serves a dual function: activation of genes expressed predominantly in PGCs, and conversely suppression of somatic genes...
January 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28045927/synbiotics-for-broiler-chickens-in-vitro-design-and-evaluation-of-the-influence-on-host-and-selected-microbiota-populations-following-in-ovo-delivery
#17
Aleksandra Dunislawska, Anna Slawinska, Katarzyna Stadnicka, Marek Bednarczyk, Piotr Gulewicz, Damian Jozefiak, Maria Siwek
Synbiotics are synergistic combinations of prebiotics and probiotics. In chickens, synbiotics can be delivered in ovo to expedite colonization of the gut by beneficial bacteria. We therefore aimed to design synbiotics in vitro and validate them in broiler chickens upon in ovo delivery. The probiotic components of the synbiotics were Lactobacillus salivarius and Lactobacillus plantarum. Their growth was assessed in MRS medium supplemented with different prebiotics. Based on in vitro results (hatchability and growth curve), two synbiotics were designed: S1 -Lactobacillus salivarius with galactooligosaccarides (GOS) and S2 -Lactobacillus plantarum with raffinose family oligosaccharides (RFO)...
2017: PloS One
https://www.readbyqxmd.com/read/28039067/prenatal-betaine-exposure-alleviates-corticosterone-induced-inhibition-of-cyp27a1-expression-in-the-liver-of-juvenile-chickens-associated-with-its-promoter-dna-methylation
#18
Yun Hu, Qinwei Sun, Yibo Zong, Jie Liu, Abdulrahman A Idriss, Nagmeldin A Omer, Ruqian Zhao
Sterol 27-hydroxylase (CYP27A1) plays an important role in cholesterol homeostasis by degrading cholesterol to bile acids. Betaine can alleviate high-fat diet-induced hepatic cholesterol accumulation and maternal betaine treatment programs the hepatic expression of CYP27A1 in offspring. Excessive corticosterone (CORT) exposure causes hepatic cholesterol deposition in chickens, yet it remains unknown whether prenatal betaine modulates CORT-induced cholesterol accumulation in chicken liver later in life and whether it involves epigenetic gene regulation of CYP27A1...
December 27, 2016: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28038868/marek-s-disease-vaccines-current-status-and-strategies-for-improvement-and-development-of-vector-vaccines
#19
Sanjay M Reddy, Yoshihiro Izumiya, Blanca Lupiani
Marek's disease (MD) is a lymphoproliferative viral disease of chickens, which has been controlled through vaccination since 1969. MD vaccines protect against tumors but do not provide sterilizing immunity, and thus it is generally believed that their use has contributed to increase virulence of field strains with the ability to cause MD in vaccinated chickens. Traditional methods of developing vaccines, like cell culture attenuation, have proved unsuccessful for the development of improved vaccines to protect against highly virulent MD virus (MDV) field strains...
November 24, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/28034921/matrix-stiffening-promotes-a-tumor-vasculature-phenotype
#20
Francois Bordeleau, Brooke N Mason, Emmanuel Macklin Lollis, Michael Mazzola, Matthew R Zanotelli, Sahana Somasegar, Joseph P Califano, Christine Montague, Danielle J LaValley, John Huynh, Nuria Mencia-Trinchant, Yashira L Negrón Abril, Duane C Hassane, Lawrence J Bonassar, Jonathan T Butcher, Robert S Weiss, Cynthia A Reinhart-King
Tumor microvasculature tends to be malformed, more permeable, and more tortuous than vessels in healthy tissue, effects that have been largely attributed to up-regulated VEGF expression. However, tumor tissue tends to stiffen during solid tumor progression, and tissue stiffness is known to alter cell behaviors including proliferation, migration, and cell-cell adhesion, which are all requisite for angiogenesis. Using in vitro, in vivo, and ex ovo models, we investigated the effects of matrix stiffness on vessel growth and integrity during angiogenesis...
January 17, 2017: Proceedings of the National Academy of Sciences of the United States of America
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